CN105613048B - A kind of method for improving rhizoma Gastrodiae protocorm and connecing bacterium rate - Google Patents

A kind of method for improving rhizoma Gastrodiae protocorm and connecing bacterium rate Download PDF

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CN105613048B
CN105613048B CN201610117675.3A CN201610117675A CN105613048B CN 105613048 B CN105613048 B CN 105613048B CN 201610117675 A CN201610117675 A CN 201610117675A CN 105613048 B CN105613048 B CN 105613048B
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protocorm
rhizoma gastrodiae
river sand
halimasch
bacterium rate
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CN105613048A (en
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何朝伦
田德连
严飞
李建华
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Guizhou Xiancaotang Pharmaceutical Technology Co Ltd
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Guizhou Xiancaotang Pharmaceutical Technology Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms

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  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Cultivation Of Plants (AREA)
  • Mushroom Cultivation (AREA)

Abstract

The present invention relates to gastrodia elata sexual propagation improving environment field, especially a kind of method for improving rhizoma Gastrodiae protocorm and connecing bacterium rate, the step of being used in the method for bacterium rate (1) is connect by improving rhizoma Gastrodiae protocorm, step (2), step (3), the processing of step (4), the opportunity that halimasch in halimasch material element growth animated period is germinateed with seeds of Gastrodia elata is met well, and combine the processing procedure of above-mentioned steps, and the restriction of parameter, the protocorm of seeds of Gastrodia elata is enabled effectively quickly to set up nutrition supply relation with halimasch material, protocorm is quickly grown, reduce the death rate of protocorm, improve its survival rate, reduce rhizoma Gastrodiae planting cost;Most prominent is enable to protocorm and halimasch material more than 2 times connecing bacterium rate and reaching prior art, and then improves more than 2 times of progress gastrodia elata sexual propagation yield in unit area so that the economic benefit of planting rhizoma gastrodiae is significantly improved.

Description

A kind of method for improving rhizoma Gastrodiae protocorm and connecing bacterium rate
Technical field
The present invention relates to gastrodia elata sexual propagation improving environment field, especially a kind of side improved rhizoma Gastrodiae protocorm and connect bacterium rate Method.
Background technology
It is well known that conventional gastrodia elata sexual propagation is after planting, the protocorm at least 93%-95% for formation of germinateing is not With halimasch material it can produce nutrition supply relation in time and dead so that effective to connect bacterium rate be only 5- by gastrodia elata sexual propagation total 7% so that every square metre of yield is only 3-5kg so that the economic benefit of rhizoma Gastrodiae plantation is relatively low, and survival rate is relatively low, cultivates into This is higher.
And the reason for causing above-mentioned technological deficiency, is:Gastrodia elata sexual propagation type of seeding under routine techniques is to sprout Strain is sowed simultaneously with cultured halimasch material, is caused after 50 days, the vigorous period of substantial amounts of halimasch material mistake, greatly The young tender halimasch material element criticized reaches maturity period and decline phase so that substantial amounts of rhizoma Gastrodiae protocorm does not have with halimasch material connects bacterium Ability and it is dead, reduce germination rate, the survival rate of gastrodia elata sexual propagation, increase rhizoma Gastrodiae planting cost.
Also this is based on, this researcher is sprouting strain to day on the basis of Patent No. 201010542943.9 Under support in numb generative propagation, the present invention passes through the time to the contact mixed processing between rhizoma Gastrodiae protocorm and halimasch material It is controlled, and combines technique for cultivation of Gastrodia elata, gastrodin cultivation method is adjusted and controlled so that rhizoma Gastrodiae is forming protocorm Afterwards, nutrition supply relation can be timely set up with halimasch material, the survival rate of rhizoma Gastrodiae protocorm is improved, reduces Gastrodia Cost is planted, a kind of new approaches are provided for rhizoma Gastrodiae planting technology field.
The content of the invention
In order to solve above-mentioned technical problem present in prior art, the present invention provides a kind of rhizoma Gastrodiae protocorm that improves and connects bacterium The method of rate.
It is achieved particular by following technical scheme:
A kind of method for improving rhizoma Gastrodiae protocorm and connecing bacterium rate, comprises the following steps:
(1) Germination Strain is prepared in the November of last year in gastrodin cultivation year;Halimasch has been cultivated in the July of grower's year Material;Refrigeration emergence is carried out in the November of grower's year and obtains arrow fiber crops;
(2) temperature is controlled between 20-28 DEG C, in the field planting rhizoma Gastrodiae arrow fiber crops on January 10 of grower's year Second Year, is opened within 1 day 2 months Beginning emergence is handled, and rhizoma Gastrodiae is pollinated at 25 days 2 months, and in harvesting ripe fruit on March 18;
(3) fruit is admixed among sprouting strain, obtains Germination Strain of dressing seed;It is used into river in breeding box or breeding bag The processing of sand-base cultivation kind, make germination in rhizoma Gastrodiae fruit into protocorm;
(4) choose sowing place, used it is brick into wide 100cm, long 200cm, high 25cm planting tank;In planting tank Bottom spread the thick river sand of one layer of 4-6cm, then spread last layer protocorm on the surface of river sand, protocorm is non-overlapping between each other; Protocorm is covered with river sand again, and cultured halimasch material is laid in above, between halimasch material is mutual Away from for 5cm, and every halimasch material covering river sand 2cm, then spread protocorm, protocorm in the mutual spacing of halimasch material Stem is non-overlapping between each other, and is spread using river sand to equal with halimasch material;Spread one layer of protocorm again, protocorm is between each other not It is overlapping, it is thick to cover protocorm 1cm with river sand, and cultured halimasch material is laid in above, halimasch material is mutual Spacing is 5cm, and every halimasch material is covered to half by river sand, then in the mutual spacing of halimasch material Protocorm is spread, protocorm is non-overlapping between each other, and is spread using river sand to equal with halimasch material;One layer of protocorm is spread again, it is former Bulb is non-overlapping between each other, then covers 10cm using river sand, and the humidity for keeping the river sand in planting tank is 55%-60%, i.e., Can.
Breeding processing is carried out using river sand in described step (3), its specific breeding processing method be in breeding bag or One layer, thickness 0.7-1.2cm of breeding box middle berth river sand, one layer of seed dressing Germination Strain is then spread, thickness is 1cm thick, is then covered again One layer of river sand, thickness 1cm, then one layer of seed dressing Germination Strain is spread, thickness 1cm, carry out successively, until by breeding bag or breeding box After filling, then the river sand in the top lid 2cm thickness, its breeding is handled 60 days, you can.
Described river sand, its humidity are 60%.
Described breeding bag is polybag;Described breeding box is bamboo and wood plastic crate.
River sand in described step (4) is substituted using one kind in char-forming material, soil.
River sand in described step (3) is substituted using one kind in char-forming material, soil.
Described char-forming material is to be processed into black wood charcoal in the burning in pond that is carbonized using husk or a diameter of 1cm wood particle Material afterwards.
Refrigeration in described step (1), be by it after storing at least 40 days during temperature is 3-4 DEG C of environment, then will Arrow fiber crops are made in its processing of emerging.
Described emergence, its environment temperature are 20-28 DEG C.
Described char-forming material, it is during burning is processed into black wood charcoal, to husk or a diameter of 1cm wood particle Middle addition has diesel oil.
Compared with prior art, there is technique effect to be embodied in for it:
The step of being used in the method for bacterium rate (1), step (2), step (3), step (4) are connect by improving rhizoma Gastrodiae protocorm Processing so that the opportunity that the halimasch element growth animated period in halimasch material can germinate with seeds of Gastrodia elata carries out good phase Meet, and combine the processing procedure of above-mentioned steps, and the restriction of parameter so that the protocorm of seeds of Gastrodia elata can be effectively quick Set up nutrition supply relation with halimasch material so that protocorm can quickly grow, and reduce the death rate of protocorm, Its survival rate is improved, reduces rhizoma Gastrodiae planting cost;Most prominent is that the bacterium rate that connects for enabling to protocorm and halimasch material reaches More than 2 times of prior art, and then improve more than 2 times of progress gastrodia elata sexual propagation yield in unit area so that plantation The economic benefit of rhizoma Gastrodiae is significantly improved.
Pass through the processing of above-mentioned technological means in the present invention so that rhizoma Gastrodiae protocorm connects bacterium rate by original 5-7% 15-21% is risen to, each square of yield has increased to 9-15kg by original 3-5kg, than original every square meter per unit area yield extremely Reduce and add 200%, effectively raise the yield for connecing bacterium rate, improving rhizoma Gastrodiae of rhizoma Gastrodiae protocorm, add economic benefit.
Embodiment
Limited with reference to specific embodiment technical scheme is further, but claimed Scope is not only limited to made description.
It is noted that corresponding undocumented technological means is phase on the basis of existing technology in present specification The vocabulary of terms and technological means for the not specified (NS) answered using Patent No. 201010542943.9 sprouting strain pair The support of gastrodia elata sexual propagation.Cultural method of the prior art during the culture of the halimasch of use, its technique of refrigeration used It is to be handled in 3-4 DEG C of storage.The Germination Strain of the present invention is that have in the sprouting strain of Patent No. 201010542943.9 to rhizoma Gastrodiae On the basis of the support of sexual reproduction, using sprouting the Germination Strain that is prepared of strain.
Embodiment 1
A kind of method for improving rhizoma Gastrodiae protocorm and connecing bacterium rate, comprises the following steps:
(1) Germination Strain is prepared in the November of last year in gastrodin cultivation year;Halimasch has been cultivated in the July of grower's year Material;Refrigeration emergence is carried out in the November of grower's year and obtains arrow fiber crops;
(2) temperature is controlled between 20-28 DEG C, in the field planting rhizoma Gastrodiae arrow fiber crops on January 10 of grower's year Second Year, is opened within 1 day 2 months Beginning emergence is handled, and rhizoma Gastrodiae is pollinated at 25 days 2 months, and in harvesting ripe fruit on March 18;
(3) fruit is admixed among sprouting strain, obtains Germination Strain of dressing seed;It is used into river in breeding box or breeding bag The processing of sand-base cultivation kind, make germination in rhizoma Gastrodiae fruit into protocorm;
(4) choose sowing place, used it is brick into wide 100cm, long 200cm, high 25cm planting tank;In planting tank Bottom spread the thick river sand of one layer of 4-6cm, then spread last layer protocorm on the surface of river sand, protocorm is non-overlapping between each other; Protocorm is covered with river sand again, and cultured halimasch material is laid in above, between halimasch material is mutual Away from for 5cm, and every halimasch material covering river sand 2cm, then spread protocorm, protocorm in the mutual spacing of halimasch material Stem is non-overlapping between each other, and is spread using river sand to equal with halimasch material;Spread one layer of protocorm again, protocorm is between each other not It is overlapping, it is thick to cover protocorm 1cm with river sand, and cultured halimasch material is laid in above, halimasch material is mutual Spacing is 5cm, and every halimasch material is covered to half by river sand, then in the mutual spacing of halimasch material Protocorm is spread, protocorm is non-overlapping between each other, and is spread using river sand to equal with halimasch material;One layer of protocorm is spread again, it is former Bulb is non-overlapping between each other, then covers 10cm using river sand, and the humidity for keeping the river sand in planting tank is 55%-60%, i.e., Can.
Embodiment 2
It is a kind of to improve the rhizoma Gastrodiae protocorm method that connects bacterium rate, on the basis of embodiment 1, other with embodiment 1,
Breeding processing is carried out using river sand in described step (3), its specific breeding processing method be in breeding bag or One layer, thickness 0.7-1.2cm of breeding box middle berth river sand, one layer of seed dressing Germination Strain is then spread, thickness is 1cm thick, is then covered again One layer of river sand, thickness 1cm, then one layer of seed dressing Germination Strain is spread, thickness 1cm, carry out successively, until by breeding bag or breeding box After filling, then the river sand in the top lid 2cm thickness, its breeding is handled 60 days, river sand humidity is 60%.
Embodiment 3
A kind of method for improving rhizoma Gastrodiae protocorm and connecing bacterium rate, on the basis of embodiment 1, other are described with embodiment 1 Breeding bag be polybag;Described breeding box is bamboo and wood plastic crate.
Refrigeration in described step (1), be by it after storing at least 40 days during temperature is 3-4 DEG C of environment, then will Arrow fiber crops are made in its processing of emerging.
Described emergence, its environment temperature are 20-28 DEG C.
Embodiment 4
A kind of method for improving rhizoma Gastrodiae protocorm and connecing bacterium rate, on the basis of embodiment 2, other are described with embodiment 2 The step of (4) in river sand substituted using char-forming material.River sand in described step (3) is substituted using char-forming material. Described char-forming material is in the thing after being processed into black wood charcoal that burns that is carbonized in pond using husk or a diameter of 1cm wood particle Material.Described char-forming material, it adds during burning is processed into black wood charcoal into husk or a diameter of 1cm wood particle Enter to have diesel oil.
Embodiment 5
A kind of method for improving rhizoma Gastrodiae protocorm and connecing bacterium rate, on the basis of embodiment 3, other are described with embodiment 3 The step of (4) in river sand substituted using soil.River sand in described step (3) is substituted using soil.
Embodiment 6
A kind of method for improving rhizoma Gastrodiae protocorm and connecing bacterium rate, on the basis of embodiment 1, other are described with embodiment 1 The step of (4) in river sand substituted using char-forming material.River sand in described step (3) is substituted using soil.It is described Char-forming material be using husk or a diameter of 1cm wood particle be carbonized in pond burn be processed into black wood charcoal after material.Institute The char-forming material stated, during burning is processed into black wood charcoal, being added into husk or a diameter of 1cm wood particle has bavin for it Oil.The addition of diesel oil is the 3-8% of the quality of husk or a diameter of 1cm wood particle.
Be necessary explanation be:It is row that concrete operations mode to the invention does exhaustive that above-described embodiment, which is not, Lift, but illustrative description and interpretation are made in the inventive concept to the invention, to help those skilled in the art to this The understanding of the technical scheme of inventive concept, unrequited endpoint value, has been enumerated in Summary in the above-described embodiments, Protection domain should be interpreted as making in this scope with Summary identical protection domain, those skilled in the art Non-protruding essential characteristics and significantly progressive improvement, belong to the protection category of the present invention.

Claims (10)

  1. A kind of 1. method for improving rhizoma Gastrodiae protocorm and connecing bacterium rate, it is characterised in that comprise the following steps:
    (1) Germination Strain is prepared in the November of last year in gastrodin cultivation year;Halimasch material has been cultivated in the July of grower's year; Refrigeration emergence is carried out in the November of grower's year and obtains arrow fiber crops;
    (2) temperature is controlled between 20-28 DEG C, and in the field planting rhizoma Gastrodiae arrow fiber crops on January 10 of grower's year Second Year, 1 day 2 months starts Seedling processing, pollinated at 25 days 2 months to rhizoma Gastrodiae, and in harvesting ripe fruit on March 18;
    (3) fruit is admixed among sprouting strain, obtains Germination Strain of dressing seed;It is educated in breeding box or breeding bag using river sand Kind of processing, makes germination in rhizoma Gastrodiae fruit into protocorm;
    (4) choose sowing place, used it is brick into wide 100cm, long 200cm, high 25cm planting tank;At the bottom of planting tank Layer spreads the thick river sands of one layer of 4-6cm, then spreads last layer protocorm on the surface of river sand, and protocorm is non-overlapping between each other;Use again River sand covers protocorm, and cultured halimasch material is laid in above, and the mutual spacing of halimasch material is 5cm, and every halimasch material covering river sand 2cm, then protocorm, protocorm phase are spread in the mutual spacing of halimasch material It is non-overlapping between mutually, and spread using river sand to equal with halimasch material;One layer of protocorm is spread again, and protocorm does not weigh between each other It is folded, protocorm 1cm thickness is covered with river sand, and cultured halimasch material is laid in above, between halimasch material is mutual Away from for 5cm, and every halimasch material is covered to half by river sand, then is spread in the mutual spacing of halimasch material Protocorm, protocorm is non-overlapping between each other, and is spread using river sand to equal with halimasch material;One layer of protocorm, protocorm are spread again Stem is non-overlapping between each other, then covers 10cm using river sand, and the humidity for keeping the river sand in planting tank is 55%-60%, you can.
  2. 2. the method that rhizoma Gastrodiae protocorm connects bacterium rate is improved as claimed in claim 1, it is characterised in that in described step (3) Breeding processing is carried out using river sand, its specific breeding processing method is in one layer of breeding bag or breeding box middle berth river sand, thickness For 0.7-1.2cm, one layer of seed dressing Germination Strain is then spread, thickness is 1cm thick, then one layer of river sand of lid, thickness 1cm again, then is spread One layer of seed dressing Germination Strain, thickness 1cm, is carried out successively, until after breeding bag or breeding box are filled, then in the top lid 2cm Thick river sand, its breeding is handled 60 days, you can.
  3. 3. the method that rhizoma Gastrodiae protocorm connects bacterium rate is improved as claimed in claim 2, it is characterised in that described river sand, its is wet Spend for 60%.
  4. 4. the method that rhizoma Gastrodiae protocorm connects bacterium rate is improved as claimed in claim 2, it is characterised in that described breeding bag is modeling Pocket;Described breeding box is bamboo and wood plastic crate.
  5. 5. the method that rhizoma Gastrodiae protocorm connects bacterium rate is improved as claimed in claim 1, it is characterised in that in described step (4) River sand substituted using one kind in char-forming material, soil.
  6. 6. the method that rhizoma Gastrodiae protocorm connects bacterium rate is improved as claimed in claim 1, it is characterised in that in described step (3) River sand substituted using one kind in char-forming material, soil.
  7. 7. the method that the raising rhizoma Gastrodiae protocorm as described in claim 5 or 6 connects bacterium rate, it is characterised in that described carbonization material Expect for the material using husk or a diameter of 1cm wood particle after the burning that is carbonized in pond is processed into black wood charcoal.
  8. 8. the method that rhizoma Gastrodiae protocorm connects bacterium rate is improved as claimed in claim 1, it is characterised in that in described step (1) Refrigeration, be by it after storing at least 40 days during temperature is 3-4 DEG C of environment, then that arrow is made is numb for processing of emerge.
  9. 9. the method that the raising rhizoma Gastrodiae protocorm as described in claim 1 or 8 connects bacterium rate, it is characterised in that described emergence, its Environment temperature is 20-28 DEG C.
  10. 10. the method that rhizoma Gastrodiae protocorm connects bacterium rate is improved as claimed in claim 7, it is characterised in that described char-forming material, During burning is processed into black wood charcoal, being added into husk or a diameter of 1cm wood particle has diesel oil for it.
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Publication number Priority date Publication date Assignee Title
CN105900693B (en) * 2016-06-06 2022-02-18 陵川县森源中药材开发有限公司 Device and method for cultivating grifola in field in basket
CN107372074B (en) * 2017-09-11 2020-03-31 云南省农业科学院药用植物研究所 Sexual propagation method for gastrodia elata seed

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CN1038743A (en) * 1988-06-24 1990-01-17 中国医学科学院药用植物资源开发研究所 Mycen osmundicola and gastrodia seed germination culture technique
CN1473459A (en) * 2003-08-07 2004-02-11 广水市东晨菌业科技有限公司 Synchronously cultivating method for rhizoma gastrodiac sexual propagation
KR20110045226A (en) * 2009-10-26 2011-05-04 박우걸 Year Round Production Of the Gastrodia elata
KR20110119352A (en) * 2010-04-27 2011-11-02 농업회사법인 애니팜(주) Liquid germiculture used gastrodia elata of cultivation method
KR20130058715A (en) * 2013-05-15 2013-06-04 조홍기 The method of gastrodia cultivation using the composition for gastrodia cultivation
CN104521708A (en) * 2014-12-09 2015-04-22 永平县永怀中药材种植有限公司 High-yield energy-saving gastrodia elata cultivation method

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1038743A (en) * 1988-06-24 1990-01-17 中国医学科学院药用植物资源开发研究所 Mycen osmundicola and gastrodia seed germination culture technique
CN1473459A (en) * 2003-08-07 2004-02-11 广水市东晨菌业科技有限公司 Synchronously cultivating method for rhizoma gastrodiac sexual propagation
KR20110045226A (en) * 2009-10-26 2011-05-04 박우걸 Year Round Production Of the Gastrodia elata
KR20110119352A (en) * 2010-04-27 2011-11-02 농업회사법인 애니팜(주) Liquid germiculture used gastrodia elata of cultivation method
KR20130058715A (en) * 2013-05-15 2013-06-04 조홍기 The method of gastrodia cultivation using the composition for gastrodia cultivation
CN104521708A (en) * 2014-12-09 2015-04-22 永平县永怀中药材种植有限公司 High-yield energy-saving gastrodia elata cultivation method

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