CN105612856A - Simple method for improving germination rate of aconitum vilmorinianum kom seeds - Google Patents

Simple method for improving germination rate of aconitum vilmorinianum kom seeds Download PDF

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Publication number
CN105612856A
CN105612856A CN201610031740.0A CN201610031740A CN105612856A CN 105612856 A CN105612856 A CN 105612856A CN 201610031740 A CN201610031740 A CN 201610031740A CN 105612856 A CN105612856 A CN 105612856A
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China
Prior art keywords
seeds
crow
dendrobium denneanum
denneanum kerr
seed
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CN201610031740.0A
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Inventor
字淑慧
沙本才
杨生超
沈勇
希丛芳
肖阳明
艾洪莲
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Yunnan Agricultural University
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Yunnan Agricultural University
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Priority to CN201610031740.0A priority Critical patent/CN105612856A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for

Abstract

The invention discloses a simple method for improving the germination rate of aconitum vilmorinianum kom seeds. The simple method comprises the following steps: (1) collecting and airing mature fruits of aconitum vilmorinianum kom to obtain and preserve the aconitum vilmorinianum kom seeds; (2) selecting a seedling bed and finishing the seedling bed; (3) drying the aconitum vilmorinianum kom seeds preserved in the step (1) in sun, performing low-temperature treatment and soaking treatment in sequence, filtering the seeds, and slightly drying the seeds in shade for later use; (4) ditching the seedling bed and sowing the seeds obtained in the step (3) in lines, and performing fine management. According to the simple method, the dormant period of the aconitum vilmorinianum kom seeds is relieved to the maximal extent, the germination time of the seeds is shortened remarkably, the germination rate of the seeds is improved, and the germination rate of the seeds is improved to be more than or equal to 90 percent.

Description

A kind of improve Dendrobium denneanum Kerr. crow and grow directly from seeds the straightforward procedure of kind of germination rate
Technical field
The present invention relates to Dendrobium denneanum Kerr. crow planting technology field, be specially and a kind of improve Dendrobium denneanum Kerr. crow and grow directly from seeds the straightforward procedure of kind of germination rate.
Background technology
The dried root that Dendrobium denneanum Kerr. crow (another name: Radix Aconiti Kusnezoffii, stifled loudspeaker, big Radix Aconiti Kusnezoffii, Radix Aconiti Vilmoriniani, Kunming Aconitum carmichjaelii Debx.) is cohosh Dendrobium denneanum Kerr. crow (AconitumvilmorinianumKomarov), for herbaceos perennial. Dendrobium denneanum Kerr. crow is herbaceos perennial, the oval spherical or Radix Dauci Sativae shape of tuber, long 2.5-7 centimetre, slightly about 1 centimetre. Stem is wound around, and up to 4 meters, dredges by the pubescence or several without hair, branch of contrary flexure. Blade heavily fortified point papery, pentagon, long 5-10 centimetre, wide 8-15.5 centimetre, base portion is felt relieved shape, and three totally cleave reaches or nearly base portion, and central authorities totally cleave sheet width rhombus, anxious sharp or short tapering, the side sheet that totally cleaves is tiltedly fan-shaped, does not wait two to split little over middle part, and the pubescence being close to is dredged on surface, and the back side is only dredged by pubescence along the pulse; Petiole is closely isometric with blade. Inflorescence has 3-6 flower; Axle and bennet are close by faint yellow contrary flexure pubescence; Bract is linear; Bennet is long 2-4 centimetre; In the middle part of the raw bennet of squamella or bottom, narrow linear, long 3-5 millimeter, wide 0.3-0.5 millimeter, close by pubescence; Sepal hyacinthine, outside is close by pubescence, upper sepal height helmet shape, high 1.7-2 centimetre, the thick 7-11 millimeter in middle part, lower edge is long 1.5-1.6 centimetre, and with the beak that outer rim forms exhibition downwards, side sepal is long 1.3-1.4 centimetre, and petal is without hair, lip is about 6 millimeters, nick, distance about 3 millimeters, bends backward; Stamen without hair, the full edge of filigree or have 2 pieces of little teeth; Carpel 5, dredges raw undercoat without hair or ovary top. Directly, without hair, long 1.6-1.8 centimetre; Seed is about 3 millimeters, prismatic, only at the one side horizontal film wing of close life. The 8-10 month blooms. Dendrobium denneanum Kerr. crow is mainly distributed on Central Yunnan and western part, the high and cold area that cools of the county such as Chuxiong, Yuxi, Binchuan, auspicious cloud, Songming, Xun Dian, Malong and the height above sea level 2100-2500m such as Sichuan (can manage) and WESTERN GUIZHOU, the shady semi-humid area of the back of the body. Dendrobium denneanum Kerr. crow bitter in the mouth, pungent, fiber crops, warm in nature, there is severe toxicity, there is expelling wind and cold, effect of removing damp and stopping pain, its medicinal part is mainly tuber, among the people for treating the diseases such as anemofrigid-damp arthralgia, paralysis due to windstroke, cardiac and abdominal pain, traumatic injury. It is mainly used in controlling traumatic injury, Fengshi Guanjie pain, deadly cold hand and foot etc. clinically. Dendrobium denneanum Kerr. crow is one of raw medicinal materials of Chinese patent medicine such as SANWUJIAO, YUNNANHONGYAOJIAONANG, ZHONGTONG CHAJI, ZHONGTONG QIWUJI, has recorded in version " Yunnan Province's Chinese crude drug standard " (medical material standard No. is cloud YNZYC-0338-2010) in 2005.
At present, destroying and artificial pernicious excavating due to growing environment, make Dendrobium denneanum Kerr. crow wild resource suffer serious destruction, medicinal raw material mostly is artificial culture kind.
Dendrobium denneanum Kerr. crow is generally adopted following several propagation method, and (1) tuber is whole broadcasts breeding method: sow with wild or tame Radix Aconiti Kusnezoffii tuber, does the single weight of tuber planted with 2-3g. According to result of the test for many years, sowing tuber is advisable with 400-450/kg, and every mu of application rate, with about 50-70kg, plants that block is excessive or too small yield is all undesirable. Although during kind of block excessive results, yield per unit area is high, but deducts application rate, its yield declines on the contrary; Although the application rate of kind of the too small unit are of block reduces, but owing to parent nutrient is under-supply, seedling is thin and delicate, and growing way is poor, yields poorly, and economic benefit is also bad. Tuber is excessive, and sowing quantity increases, cost up; Too small, although sowing quantity reduces, and cost is low, but growth of emerging is poor, and economic benefit is bad, and the magnitude relationship of Radix Aconiti Kusnezoffii tuber the height of yield. (2) tuber cuts bud breeding method: after receipts are dug, the cutter after sterilizing with ethanol or plant ash, is cut by bigger Radix Aconiti Kusnezoffii tuber top terminal bud about 2-3cm and does kind of a use, otch is spilt plant ash or uses antibacterial surface sterilization, drying surface use to be sowed. Commodity processing is done in the tuber bottom of excision. It has been experienced that, yield is had no significant effect. (3) axillalry bud fruit breeding method: generally adopt and broadcast sowing, be advisable with 400/m2, broadcast rear earthing about 3cm, can transplant for after planting 90-120 days, height of seedling 15-20cm during transplanting.
But, on producing, adopt above asexual propagation, by the restriction of cultivation condition and geographical position, cost remains high. It is additionally, since soil continuous cropping and cultivar is single, cause Dendrobium denneanum Kerr. crow downy mildew, root rot etc. day by day serious, limit the development of Dendrobium denneanum Kerr. crow. Not high as amphigenetic seminal propagation germination rate, it is difficult to select high-quality, disease-resistant variety, also it is the key constraints of Dendrobium denneanum Kerr. crow large-scale promotion. And adopt Dendrobium denneanum Kerr. crow to grow directly from seeds and kind breed, germination rate, survival rate are relatively low.
Summary of the invention
Technical problem solved by the invention is in that to provide a kind of and improves Dendrobium denneanum Kerr. crow and grow directly from seeds the straightforward procedure of kind of germination rate, thus the problem solved in above-mentioned background technology.
Technical problem solved by the invention realizes by the following technical solutions:
A kind of improve Dendrobium denneanum Kerr. crow and grow directly from seeds the straightforward procedure of kind of germination rate, comprise the steps:
(1) gather Dendrobium denneanum Kerr. crow mature fruit, carry out dry in the sun in the sun, obtain Dendrobium denneanum Kerr. crow and grow directly from seeds and kind collect;
(2) select seedbed, and carry out whole moisture in the soil;
(3) kind of being grown directly from seeds by the Dendrobium denneanum Kerr. crow that step (1) is collected carries out, according to shining, then sequentially carrying out K cryogenic treatment, immersion treatment, filtering out seed, dry slightly in the cool, standby;
(4) seed step (3) obtained is ditched drilling on the seedbed of step (2), and carries out precision management.
In the present invention, as the preferred technical scheme of one, in step (1), the acquisition time of described Dendrobium denneanum Kerr. crow mature fruit is chosen as the 10-11 month, and collection appearance color is yellowish-brown but not opening mature fruit.
In the present invention, as a kind of it is preferred that technical scheme, in step (1), the described Dendrobium denneanum Kerr. crow mature fruit collected is placed on sun-drenched local dry in the sun, shell is opened in solarization, allows seed shrug off shell after naturally shelling, removes impurity, obtain cleanliness to reach the Dendrobium denneanum Kerr. crow of more than 90% and grow directly from seeds kind, be then concealed in dry cool place place.
In the present invention, as the preferred technical scheme of one, in step (2), described seedbed select not plant in 2-3 aconitum plant, leeward on the sunny side, irrigation and drainage are convenient, the soil is porous, water-retaining property is strong, rich in the sandy loam of humus or Evening Tide soil.
In the present invention, as a kind of it is preferred that technical scheme, in step (2), described sandy loam or Evening Tide soil are deep ploughed 15-20 centimetre prior to seeding for 1 week, spread fertilizer over the fields well-rotted farmyard manure 2000-2200 in small, broken bits thousand grams/acre, described farm manure and soil are sufficiently mixed, after the thin leveling of rake, make wide 1.0��1.2 meters of flat furrow or high furrow, it is watered sprinkling moisture in the soil face with 40wt% formalin again and carries out soil sterilization, obtain the seedbed put in order.
Farm manure can adopt the feces after becoming thoroughly decomposed, plant green manure etc.
In the present invention, as the preferred technical scheme of one, in step (3), take out the seed of collection prior to seeding, shine 5-7 hour in the sun, and frequently stir, it is ensured that all seeds obtain according to shining.
In the present invention, as a kind of it is preferred that technical scheme, in step (3), by according to the seed after shining 5 DEG C of K cryogenic treatment 35 days, then seed is placed on the water yield be seed volume 3-5 times, temperature is immersion 20-24 hour in 20-25 DEG C of water, and period with the hands washed by rubbing with the hands every 5-6 hour, and change water 4-6 time, it is then filtered off seed, dries slightly in the cool, standby.
In the present invention, as the preferred technical scheme of one, in step (4), the sowing time of trench digging drilling is chosen as March at the beginning of 4 months.
In the present invention, as a kind of it is preferred that technical scheme, in step (4), trench digging drilling sowing quantity be 4.5-5kg/ mu.
In the present invention, as a kind of it is preferred that technical scheme, in step (4), during trench digging drilling, seedbed adopts line-spacing 15cm, deep 5cm mode ditch, by immersion treatment, dry after seed and the mixing of a small amount of fine sand, broadcast sowing in ditch by every square metre of 6-7g application rate, blinding 3cm-4cm, then cover lid layer green Folium Pini or grass, it is beneficial to moisturizing and waters.
A small amount of fine sand refers to that the weight ratio of seed and fine sand is 3:1.
In the present invention, as a kind of it is preferred that technical scheme, in step (4), in after planting 30-45 days, period keeps seedbed ambient temperature to be 18-22 DEG C, and the water content of nursery soil is 50-60wt%, it is ensured that required moisture of emerging.
In the present invention, as a kind of it is preferred that technical scheme, in step (4), the weeds on front seedbed that emerge can carry out weeding with 41wt% glyphosate, need after emerging adopt artificial weeding, it is ensured that neat and consistent of emerging. Wind in the spring a small amount of soil of big application pushes down bed surface covering, it is prevented that run by wind.
In the present invention, as a kind of it is preferred that technical scheme, in step (4), seedbed early stage utilizes sunshade net to shelter from heat or light, and when seedling true leaf reaches 2-3 sheet, more slowly removes sunshade net.
Owing to have employed above technical scheme, the method have the advantages that
Inventor is through a large amount of tests repeatedly, comprehensive screening has been carried out on affecting the grow directly from seeds various factors of kind of germination rate of Dendrobium denneanum Kerr. crow, finally draw technical scheme, and propose Dendrobium denneanum Kerr. crow targetedly and grow directly from seeds and kind at seeding time seedbed specifically selected, prepare, arrange, and kind that Dendrobium denneanum Kerr. crow is grown directly from seeds carries out specifically gathering, collecting, and Dendrobium denneanum Kerr. crow is grown directly from seeds kind carry out strict pretreatment prior to seeding, after planting it is being also carried out the management that becomes more meticulous.
The method utilizing the present invention, maximum limit relieve the rest period that Dendrobium denneanum Kerr. crow is grown directly from seeds kind, work shortens the sprout time of seed, improves the germination rate of seed, and the germination rate of seed is increased to more than 90%.
Detailed description of the invention
For the technological means making the present invention realize, creation characteristic, reach purpose and effect and be easy to understand, below in conjunction with specific embodiment, the present invention is expanded on further.
Embodiment 1
A kind of improve Dendrobium denneanum Kerr. crow and grow directly from seeds the straightforward procedure of kind of germination rate, comprise the steps:
(1) Dendrobium denneanum Kerr. crow mature fruit is gathered, and carry out dry in the sun in the sun, the acquisition time of described Dendrobium denneanum Kerr. crow mature fruit is chosen as October, and collection appearance color is yellowish-brown but not opening mature fruit, the described Dendrobium denneanum Kerr. crow mature fruit collected is placed on sun-drenched local dry in the sun, shines and open shell, seed is allowed to shrug off shell after naturally shelling, remove impurity, obtain cleanliness and reach the Dendrobium denneanum Kerr. crow of 90% and grow directly from seeds kinds, the Dendrobium denneanum Kerr. crow obtained is grown directly from seeds and kind is concealed in dry shady and cool locate;
(2) seedbed is selected, and carry out whole moisture in the soil, in this step, described seedbed select not plant in 2 years aconitum plant, leeward on the sunny side, irrigation and drainage are convenient, the soil is porous, water-retaining property is strong, rich in the sandy loam of humus or Evening Tide soil, described sandy loam or Evening Tide soil are deep ploughed 15 centimetres prior to seeding for 1 week, spread fertilizer over the fields well-rotted farmyard manure in small, broken bits 2,000,000 grams/acre, described farm manure and soil are sufficiently mixed, wide 1.0 meters of flat furrow or high furrow are made after the thin leveling of rake, it is watered sprinkling moisture in the soil face with 40wt% formalin again and carries out soil sterilization, obtain the seedbed put in order;
(3) kind of being grown directly from seeds by the Dendrobium denneanum Kerr. crow that step (1) is collected carries out according to shining, namely the seed of collection is taken out prior to seeding, shine 5 hours in the sun, and frequently stir, ensure that all seeds obtain according to shining, then K cryogenic treatment will sequentially be carried out according to after shining, immersion treatment, wherein, K cryogenic treatment refers to low-temp storage 35 days at 5 DEG C, immersion treatment refers to that it is seed volume 3 times that seed is placed on the water yield, temperature is soak 20 hours in 20 DEG C of water, period with the hands washed (washing by rubbing with the hands last after 20 hours) by rubbing with the hands every 5 hours, and change water 4 times, it is then filtered off seed, and after utilizing gibberellins to soak seed 24h, filter out seed, dry slightly in the cool, standby,
(4) seed step (3) obtained is ditched drilling on the seedbed of step (2), and carry out precision management, the sowing time of trench digging drilling is chosen as March, the sowing quantity of trench digging drilling is 4.5kg/ mu, during trench digging drilling, seedbed adopts line-spacing 15cm, deep 5cm mode is ditched, by immersion treatment, seed after drying and the mixing of a small amount of fine sand, broadcast sowing in ditch by every square metre of 6g application rate, blinding 3cm, cover lid layer green Folium Pini or grass again, it is beneficial to moisturizing and waters, in after planting 30 days, period keeps temperature to be 18 DEG C, the water content of nursery soil is 50wt%, ensure to emerge required moisture, the weeds on front seedbed that emerge can carry out weeding with 41wt% glyphosate, need after emerging to adopt artificial weeding, ensure to emerge neat and consistent. wind in the spring a small amount of soil of big application pushes down bed surface covering, it is prevented that being run by wind, seedbed early stage utilizes sunshade net to shelter from heat or light, and when seedling true leaf reaches 2, more slowly removes sunshade net.
The Dendrobium denneanum Kerr. crow of the present embodiment sowing is grown directly from seeds kind, and germination rate is 90%.
Embodiment 2
A kind of improve Dendrobium denneanum Kerr. crow and grow directly from seeds the straightforward procedure of kind of germination rate, comprise the steps:
(1) Dendrobium denneanum Kerr. crow mature fruit is gathered, carry out dry in the sun in the sun, the acquisition time of described Dendrobium denneanum Kerr. crow mature fruit is chosen as November, and collection appearance color is yellowish-brown but not opening mature fruit, the described Dendrobium denneanum Kerr. crow mature fruit collected is placed on sun-drenched local dry in the sun, shines and open shell, seed is allowed to shrug off shell after naturally shelling, remove impurity, obtain cleanliness and reach the Dendrobium denneanum Kerr. crow of 90% and grow directly from seeds kinds, the Dendrobium denneanum Kerr. crow obtained is grown directly from seeds and kind is concealed in dry shady and cool locate;
(2) seedbed is selected, and carry out whole moisture in the soil, in this step, described seedbed select not plant in 2 years aconitum plant, leeward on the sunny side, irrigation and drainage are convenient, the soil is porous, water-retaining property is strong, rich in the sandy loam of humus or Evening Tide soil, described sandy loam or Evening Tide soil are deep ploughed 20 centimetres prior to seeding for 1 week, spread fertilizer over the fields well-rotted farmyard manure in small, broken bits 2,200,000 grams/acre, described farm manure and soil are sufficiently mixed, wide 1.2 meters of flat furrow or high furrow are made after the thin leveling of rake, it is watered sprinkling moisture in the soil face with 40wt% formalin again and carries out soil sterilization, obtain the seedbed put in order;
(3) kind of being grown directly from seeds by the Dendrobium denneanum Kerr. crow that step (1) is collected carries out according to shining, namely the seed of collection is taken out prior to seeding, shine 7 hours in the sun, and frequently stir, ensure that all seeds obtain according to shining, then K cryogenic treatment will sequentially be carried out according to after shining, immersion treatment, wherein, K cryogenic treatment refers to low-temp storage 35 days at 5 DEG C, immersion treatment refers to that it is seed volume 5 times that seed is placed on the water yield, temperature is soak 24 hours in 25 DEG C of water, period with the hands washed (washing by rubbing with the hands last after 24 hours) by rubbing with the hands every 5 hours, and change water 6 times, it is then filtered off seed, dry slightly in the cool, standby,
(4) seed step (3) obtained is ditched drilling on the seedbed of step (2), and carry out precision management, the sowing time of trench digging drilling is chosen as at the beginning of 4 months, the sowing quantity of trench digging drilling is 4.5-5kg/ mu, during trench digging drilling, seedbed adopts line-spacing 15cm, deep 5cm mode is ditched, by immersion treatment, seed after drying and the mixing of a small amount of fine sand, broadcast sowing in ditch by every square metre of 7g application rate, blinding 4cm, cover lid layer green Folium Pini or grass again, it is beneficial to moisturizing and waters, in after planting 45 days, period keeps temperature to be 22 DEG C, the water content of nursery soil is 60wt%, ensure to emerge required moisture, the weeds on front seedbed that emerge can carry out weeding with 41wt% glyphosate, need after emerging to adopt artificial weeding, ensure to emerge neat and consistent. wind in the spring a small amount of soil of big application pushes down bed surface covering, it is prevented that being run by wind, seedbed early stage utilizes sunshade net to shelter from heat or light, and when seedling true leaf reaches 3, more slowly removes sunshade net.
The Dendrobium denneanum Kerr. crow of the present embodiment sowing is grown directly from seeds kind, and germination rate is 91%.
Embodiment 3
A kind of improve Dendrobium denneanum Kerr. crow and grow directly from seeds the straightforward procedure of kind of germination rate, comprise the steps:
(1) Dendrobium denneanum Kerr. crow mature fruit is gathered, and carry out dry in the sun in the sun, the acquisition time of described Dendrobium denneanum Kerr. crow mature fruit is chosen as mid-October, and collection appearance color is yellowish-brown but not opening mature fruit, the described Dendrobium denneanum Kerr. crow mature fruit collected is placed on sun-drenched local dry in the sun, shines and open shell, seed is allowed to shrug off shell after naturally shelling, remove impurity, obtain cleanliness and reach the Dendrobium denneanum Kerr. crow of 95% and grow directly from seeds kinds, the Dendrobium denneanum Kerr. crow obtained is grown directly from seeds and kind is concealed in dry shady and cool locate;
(2) seedbed is selected, and carry out whole moisture in the soil, in this step, described seedbed select not plant in 3 years aconitum plant, leeward on the sunny side, irrigation and drainage are convenient, the soil is porous, water-retaining property is strong, rich in the sandy loam of humus or Evening Tide soil, described sandy loam or Evening Tide soil are deep ploughed 18 centimetres prior to seeding for 1 week, spread fertilizer over the fields well-rotted farmyard manure in small, broken bits 2,100,000 grams/acre, described farm manure and soil are sufficiently mixed, wide 1.2 meters of flat furrow or high furrow are made after the thin leveling of rake, it is watered sprinkling moisture in the soil face with 40wt% formalin again and carries out soil sterilization, obtain the seedbed put in order;
(3) kind of being grown directly from seeds by the Dendrobium denneanum Kerr. crow that step (1) is collected carries out according to shining, namely the seed of collection is taken out prior to seeding, shine 6 hours in the sun, and frequently stir, ensure that all seeds obtain according to shining, then K cryogenic treatment will sequentially be carried out according to after shining, immersion treatment, wherein, K cryogenic treatment refers to low-temp storage 35 days at 5 DEG C, immersion treatment refers to that it is seed volume 4 times that seed is placed on the water yield, temperature is soak 24 hours in 23 DEG C of water, period with the hands washed (washing by rubbing with the hands last after 24 hours) by rubbing with the hands every 5 hours, and change water 5 times, it is then filtered off seed, and after utilizing gibberellins to soak seed 24h, filter out seed, dry slightly in the cool, standby,
(4) seed step (3) obtained is ditched drilling on the seedbed of step (2), and carry out precision management, the sowing time of trench digging drilling is chosen as mid-March, the sowing quantity of trench digging drilling is 4.8kg/ mu, during trench digging drilling, seedbed adopts line-spacing 15cm, deep 5cm mode is ditched, by immersion treatment, seed after drying and the mixing of a small amount of fine sand, broadcast sowing in ditch by every square metre of 6.5g application rate, blinding 3.5cm, cover lid layer green Folium Pini or grass again, it is beneficial to moisturizing and waters, in after planting 40 days, period keeps temperature to be 20 DEG C, the water content of nursery soil is 55wt%, ensure to emerge required moisture, the weeds on front seedbed that emerge can carry out weeding with 41wt% glyphosate, need after emerging to adopt artificial weeding, ensure to emerge neat and consistent. wind in the spring a small amount of soil of big application pushes down bed surface covering, it is prevented that being run by wind, seedbed early stage utilizes sunshade net to shelter from heat or light, and when seedling true leaf reaches 3, more slowly removes sunshade net.
The Dendrobium denneanum Kerr. crow of the present embodiment sowing is grown directly from seeds kind, and germination rate is 96%.
Embodiment 4
A kind of improve Dendrobium denneanum Kerr. crow and grow directly from seeds the straightforward procedure of kind of germination rate, comprise the steps:
(1) Dendrobium denneanum Kerr. crow mature fruit is gathered, carry out dry in the sun in the sun, the acquisition time of described Dendrobium denneanum Kerr. crow mature fruit is chosen as November, and collection appearance color is yellowish-brown but not opening mature fruit, the described Dendrobium denneanum Kerr. crow mature fruit collected is placed on sun-drenched local dry in the sun, shines and open shell, seed is allowed to shrug off shell after naturally shelling, remove impurity, obtain cleanliness and reach the Dendrobium denneanum Kerr. crow of 90% and grow directly from seeds kinds, the Dendrobium denneanum Kerr. crow obtained is grown directly from seeds and kind is concealed in dry shady and cool locate;
(2) seedbed is selected, and carry out whole moisture in the soil, in this step, described seedbed select not plant in 2 years aconitum plant, leeward on the sunny side, irrigation and drainage are convenient, the soil is porous, water-retaining property is strong, rich in the sandy loam of humus or Evening Tide soil, described sandy loam or Evening Tide soil are deep ploughed 20 centimetres prior to seeding for 1 week, spread fertilizer over the fields well-rotted farmyard manure in small, broken bits 2,000,000 grams/acre, described farm manure and soil are sufficiently mixed, wide 1.1 meters of flat furrow or high furrow are made after the thin leveling of rake, it is watered sprinkling moisture in the soil face with 40wt% formalin again and carries out soil sterilization, obtain the seedbed put in order;
(3) kind of being grown directly from seeds by the Dendrobium denneanum Kerr. crow that step (1) is collected carries out according to shining, namely the seed of collection is taken out prior to seeding, shine 6 hours in the sun, and frequently stir, ensure that all seeds obtain according to shining, then K cryogenic treatment will sequentially be carried out according to after shining, immersion treatment, wherein, K cryogenic treatment refers to low-temp storage 35 days at 5 DEG C, immersion treatment refers to that it is seed volume 3 times that seed is placed on the water yield, temperature is soak 24 hours in 25 DEG C of water, period with the hands washed by rubbing with the hands every 6 hours, and change water 5 times, it is then filtered off seed, dry slightly in the cool, standby,
(4) seed step (3) obtained is ditched drilling on the seedbed of step (2), and carry out precision management, the sowing time of trench digging drilling is chosen as at the beginning of 4 months, the sowing quantity of trench digging drilling is 5kg/ mu, during trench digging drilling, seedbed adopts line-spacing 15cm, deep 5cm mode is ditched, by immersion treatment, seed after drying and the mixing of a small amount of fine sand, broadcast sowing in ditch by every square metre of 7g application rate, blinding 3.7cm, cover lid layer green Folium Pini or grass again, it is beneficial to moisturizing and waters, in after planting 35 days, period keeps temperature to be 19 DEG C, the water content of nursery soil is 57wt%, ensure to emerge required moisture, the weeds on front seedbed that emerge can carry out weeding with 41wt% glyphosate, need after emerging to adopt artificial weeding, ensure to emerge neat and consistent. wind in the spring a small amount of soil of big application pushes down bed surface covering, it is prevented that being run by wind, seedbed early stage utilizes sunshade net to shelter from heat or light, and when seedling true leaf reaches 2, more slowly removes sunshade net.
The Dendrobium denneanum Kerr. crow of the present embodiment sowing is grown directly from seeds kind, and germination rate is 92%.
Embodiment 5
A kind of improve Dendrobium denneanum Kerr. crow and grow directly from seeds the straightforward procedure of kind of germination rate, comprise the steps:
(1) Dendrobium denneanum Kerr. crow mature fruit is gathered, and carry out dry in the sun in the sun, the acquisition time of described Dendrobium denneanum Kerr. crow mature fruit is chosen as at the beginning of 10 months, and collection appearance color is yellowish-brown but not opening mature fruit, the described Dendrobium denneanum Kerr. crow mature fruit collected is placed on sun-drenched local dry in the sun, shines and open shell, seed is allowed to shrug off shell after naturally shelling, remove impurity, obtain cleanliness and reach the Dendrobium denneanum Kerr. crow of 90% and grow directly from seeds kinds, the Dendrobium denneanum Kerr. crow obtained is grown directly from seeds and kind is concealed in dry shady and cool locate;
(2) seedbed is selected, and carry out whole moisture in the soil, in this step, described seedbed select not plant in 3 years aconitum plant, leeward on the sunny side, irrigation and drainage are convenient, the soil is porous, water-retaining property is strong, rich in the sandy loam of humus or Evening Tide soil, described sandy loam or Evening Tide soil are deep ploughed 18 centimetres prior to seeding for 1 week, spread fertilizer over the fields well-rotted farmyard manure in small, broken bits 2,150,000 grams/acre, described farm manure and soil are sufficiently mixed, wide 1.2 meters of flat furrow or high furrow are made after the thin leveling of rake, it is watered sprinkling moisture in the soil face with 40wt% formalin again and carries out soil sterilization, obtain the seedbed put in order;
(3) kind of being grown directly from seeds by the Dendrobium denneanum Kerr. crow that step (1) is collected carries out according to shining, namely the seed of collection is taken out prior to seeding, shine 6 hours in the sun, and frequently stir, ensure that all seeds obtain according to shining, then K cryogenic treatment will sequentially be carried out according to after shining, immersion treatment, wherein, K cryogenic treatment refers to low-temp storage 35 days at 5 DEG C, immersion treatment refers to that it is seed volume 5 times that seed is placed on the water yield, temperature is soak 24 hours in 24 DEG C of water, period with the hands washed (washing by rubbing with the hands last after 24 hours) by rubbing with the hands every 5 hours, after change water 5 times, it is then filtered off seed, dry slightly in the cool, standby,
(4) seed step (3) obtained is ditched drilling on the seedbed of step (2), and carry out precision management, the sowing time of trench digging drilling is chosen as at the beginning of 3 months, the sowing quantity of trench digging drilling is 5kg/ mu, during trench digging drilling, seedbed adopts line-spacing 15cm, deep 5cm mode is ditched, by immersion treatment, seed after drying and the mixing of a small amount of fine sand, broadcast sowing in ditch by every square metre of 6.8g application rate, blinding 3cm-4cm, cover lid layer green Folium Pini or grass again, it is beneficial to moisturizing and waters, in after planting 43 days, period keeps temperature to be 20 DEG C, the water content of nursery soil is 57wt%, ensure to emerge required moisture, the weeds on front seedbed that emerge can carry out weeding with 41wt% glyphosate, need after emerging to adopt artificial weeding, ensure to emerge neat and consistent. wind in the spring a small amount of soil of big application pushes down bed surface covering, it is prevented that being run by wind, seedbed early stage utilizes sunshade net to shelter from heat or light, and when seedling true leaf reaches 2, more slowly removes sunshade net.
The Dendrobium denneanum Kerr. crow of the present embodiment sowing is grown directly from seeds kind, and germination rate is 92%.
The ultimate principle of the present invention and principal character and advantages of the present invention have more than been shown and described. Skilled person will appreciate that of the industry; the present invention is not restricted to the described embodiments; described in above-described embodiment and description is that principles of the invention is described; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements both fall within the claimed scope of the invention. Claimed scope is defined by appending claims and equivalent thereof.

Claims (10)

1. one kind is improved Dendrobium denneanum Kerr. crow and grows directly from seeds the straightforward procedure of kind of germination rate, it is characterised in that: comprise the steps:
(1) gather Dendrobium denneanum Kerr. crow mature fruit, carry out dry in the sun in the sun, obtain Dendrobium denneanum Kerr. crow and grow directly from seeds and kind collect;
(2) select seedbed, and carry out whole moisture in the soil;
(3) kind of being grown directly from seeds by the Dendrobium denneanum Kerr. crow that step (1) is collected carries out, according to shining, then sequentially carrying out K cryogenic treatment, immersion treatment, filtering out seed, dry slightly in the cool, standby;
(4) seed step (3) obtained is ditched drilling on the seedbed of step (2), and carries out precision management.
2. according to claim 1 a kind of improve Dendrobium denneanum Kerr. crow and grow directly from seeds the straightforward procedure of kind of germination rate, it is characterized in that: in step (1), the acquisition time of described Dendrobium denneanum Kerr. crow mature fruit is chosen as the 10-11 month, and collection appearance color is yellowish-brown but not opening mature fruit, the described Dendrobium denneanum Kerr. crow mature fruit collected is placed on sun-drenched local dry in the sun, shell is opened in solarization, seed is allowed to shrug off shell after naturally shelling, remove impurity, obtain cleanliness to reach the Dendrobium denneanum Kerr. crow of more than 90% and grow directly from seeds kind, be then concealed in dry cool place place.
3. according to claim 1 a kind of improve Dendrobium denneanum Kerr. crow and grow directly from seeds the straightforward procedure of kind of germination rate, it is characterized in that: in step (2), described seedbed selects not plant aconitum plant in 2-3, leeward on the sunny side, irrigation and drainage are convenient, the soil is porous, water-retaining property is strong, sandy loam or Evening Tide soil rich in humus, described sandy loam or Evening Tide soil are deep ploughed 15-20 centimetre prior to seeding for 1 week, spread fertilizer over the fields well-rotted farmyard manure 2000-2200 in small, broken bits thousand grams/acre, described farm manure and soil are sufficiently mixed, wide 1.0��1.2 meters of flat furrow or high furrow are made after the thin leveling of rake, it is watered sprinkling moisture in the soil face with 40wt% formalin again and carries out soil sterilization, obtain the seedbed put in order.
4. according to claim 1 a kind of improve Dendrobium denneanum Kerr. crow and grow directly from seeds the straightforward procedure of kind of germination rate, it is characterized in that: in step (3), take out the seed of collection prior to seeding, shine 5-7 hour in the sun, and frequently stir, it is ensured that all seeds obtain according to shining.
5. according to claim 4 a kind of improve Dendrobium denneanum Kerr. crow and grow directly from seeds the straightforward procedure of kind of germination rate, it is characterized in that: by according to the seed after shining 5 DEG C of K cryogenic treatment 35 days, it be seed volume 3-5 times, temperature is soak 20-24 hour in 20-25 DEG C of water that seed is placed on the water yield again, period with the hands washed by rubbing with the hands every 5-6 hour, and change water 4-6 time, it is then filtered off seed, dries slightly in the cool, standby.
6. according to claim 1 a kind of improve Dendrobium denneanum Kerr. crow and grow directly from seeds the straightforward procedure of kind of germination rate, it is characterised in that: in step (4), the sowing time of trench digging drilling is chosen as March at the beginning of 4 months.
7. according to claim 6 a kind of improve Dendrobium denneanum Kerr. crow and grow directly from seeds the straightforward procedure of kind of germination rate, it is characterised in that: the sowing quantity of trench digging drilling is 4.5-5kg/ mu.
8. according to claim 7 a kind of improve Dendrobium denneanum Kerr. crow and grow directly from seeds the straightforward procedure of kind of germination rate, it is characterized in that: during trench digging drilling, seedbed adopt line-spacing 15cm, deep 5cm mode ditch, by immersion treatment, dry after the mixing of seed and a small amount of fine sand, broadcast sowing in ditch by every square metre of 6-7g application rate, blinding 3cm-4cm, cover lid layer green Folium Pini or grass again, in after planting 30-45 days, period keeps seedbed ambient temperature to be 18-22 DEG C, and the water content of nursery soil is 50-60wt%.
9. according to claim 8 a kind of improve Dendrobium denneanum Kerr. crow and grow directly from seeds the straightforward procedure of kind of germination rate, it is characterised in that: before emerging, the 41wt% glyphosate of the weeds on seedbed carries out weeding, needs to adopt artificial weeding after emerging.
10. according to claim 9 a kind of improve Dendrobium denneanum Kerr. crow and grow directly from seeds the straightforward procedure of kind of germination rate, it is characterised in that: early stage seedbed utilizes sunshade net to shelter from heat or light, and when seedling true leaf reaches 2-3 sheet, then removes sunshade net.
CN201610031740.0A 2016-01-18 2016-01-18 Simple method for improving germination rate of aconitum vilmorinianum kom seeds Pending CN105612856A (en)

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CN106258884A (en) * 2016-08-15 2017-01-04 宣威市龙津生物科技有限责任公司 The method carrying out expanding propagation with Dendrobium denneanum Kerr. crow seed
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Application publication date: 20160601