CN105403707A - 一种检测靶物质的信号放大方法及使用该方法的免疫层析试纸和装置 - Google Patents
一种检测靶物质的信号放大方法及使用该方法的免疫层析试纸和装置 Download PDFInfo
- Publication number
- CN105403707A CN105403707A CN201510632056.3A CN201510632056A CN105403707A CN 105403707 A CN105403707 A CN 105403707A CN 201510632056 A CN201510632056 A CN 201510632056A CN 105403707 A CN105403707 A CN 105403707A
- Authority
- CN
- China
- Prior art keywords
- biomolecule
- molecule
- target material
- pad
- specific binding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000012360 testing method Methods 0.000 title claims abstract description 65
- 238000000034 method Methods 0.000 title claims abstract description 53
- 239000013076 target substance Substances 0.000 title abstract description 18
- 230000003321 amplification Effects 0.000 title abstract description 8
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract description 8
- 238000001514 detection method Methods 0.000 claims abstract description 70
- 239000002245 particle Substances 0.000 claims abstract description 49
- 239000013077 target material Substances 0.000 claims description 70
- 230000009870 specific binding Effects 0.000 claims description 34
- 238000006243 chemical reaction Methods 0.000 claims description 24
- 238000004587 chromatography analysis Methods 0.000 claims description 21
- 230000008878 coupling Effects 0.000 claims description 18
- 238000010168 coupling process Methods 0.000 claims description 18
- 238000005859 coupling reaction Methods 0.000 claims description 18
- 230000000274 adsorptive effect Effects 0.000 claims description 17
- 238000011026 diafiltration Methods 0.000 claims description 17
- 239000007788 liquid Substances 0.000 claims description 13
- 239000012528 membrane Substances 0.000 claims description 11
- 238000003317 immunochromatography Methods 0.000 claims description 9
- 102000004169 proteins and genes Human genes 0.000 claims description 9
- 108090000623 proteins and genes Proteins 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 102000039446 nucleic acids Human genes 0.000 claims description 6
- 108020004707 nucleic acids Proteins 0.000 claims description 6
- 150000007523 nucleic acids Chemical class 0.000 claims description 6
- 230000035945 sensitivity Effects 0.000 abstract description 8
- 239000000126 substance Substances 0.000 abstract description 2
- 238000002360 preparation method Methods 0.000 description 28
- 238000005507 spraying Methods 0.000 description 27
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 18
- 239000000084 colloidal system Substances 0.000 description 16
- 239000010931 gold Substances 0.000 description 16
- 229910052737 gold Inorganic materials 0.000 description 16
- 239000000243 solution Substances 0.000 description 11
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 10
- 230000001745 anti-biotin effect Effects 0.000 description 9
- 229920000728 polyester Polymers 0.000 description 9
- 239000005018 casein Substances 0.000 description 8
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 8
- 235000021240 caseins Nutrition 0.000 description 8
- 239000002158 endotoxin Substances 0.000 description 8
- 229920006008 lipopolysaccharide Polymers 0.000 description 8
- 239000006228 supernatant Substances 0.000 description 8
- 241000589562 Brucella Species 0.000 description 7
- 241001333951 Escherichia coli O157 Species 0.000 description 7
- 206010064097 avian influenza Diseases 0.000 description 7
- 239000001913 cellulose Substances 0.000 description 7
- 229920002678 cellulose Polymers 0.000 description 7
- 235000018102 proteins Nutrition 0.000 description 7
- 239000000725 suspension Substances 0.000 description 7
- 241001494479 Pecora Species 0.000 description 6
- 229920001213 Polysorbate 20 Polymers 0.000 description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 6
- 229930006000 Sucrose Natural products 0.000 description 6
- 239000000427 antigen Substances 0.000 description 6
- 102000036639 antigens Human genes 0.000 description 6
- 108091007433 antigens Proteins 0.000 description 6
- 229960002685 biotin Drugs 0.000 description 6
- 235000020958 biotin Nutrition 0.000 description 6
- 239000011616 biotin Substances 0.000 description 6
- 238000010790 dilution Methods 0.000 description 6
- 239000012895 dilution Substances 0.000 description 6
- 239000002504 physiological saline solution Substances 0.000 description 6
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 6
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 6
- 239000005720 sucrose Substances 0.000 description 6
- 241000712461 unidentified influenza virus Species 0.000 description 6
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 5
- 229940098773 bovine serum albumin Drugs 0.000 description 5
- 239000003365 glass fiber Substances 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 239000007921 spray Substances 0.000 description 5
- 241000283973 Oryctolagus cuniculus Species 0.000 description 4
- UQLDLKMNUJERMK-UHFFFAOYSA-L di(octadecanoyloxy)lead Chemical compound [Pb+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O UQLDLKMNUJERMK-UHFFFAOYSA-L 0.000 description 4
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 4
- 210000002966 serum Anatomy 0.000 description 4
- 239000007790 solid phase Substances 0.000 description 4
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 241000712431 Influenza A virus Species 0.000 description 3
- 241000700605 Viruses Species 0.000 description 3
- 238000000502 dialysis Methods 0.000 description 3
- 208000002672 hepatitis B Diseases 0.000 description 3
- 239000012535 impurity Substances 0.000 description 3
- 239000004816 latex Substances 0.000 description 3
- 229920000126 latex Polymers 0.000 description 3
- 235000013336 milk Nutrition 0.000 description 3
- 239000008267 milk Substances 0.000 description 3
- 210000004080 milk Anatomy 0.000 description 3
- 101150004747 omp31 gene Proteins 0.000 description 3
- 210000002381 plasma Anatomy 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 230000003612 virological effect Effects 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 241000555268 Dendroides Species 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 2
- 208000005176 Hepatitis C Diseases 0.000 description 2
- 108700039791 Hepatitis C virus nucleocapsid Proteins 0.000 description 2
- 241000713196 Influenza B virus Species 0.000 description 2
- 208000002979 Influenza in Birds Diseases 0.000 description 2
- 241000186779 Listeria monocytogenes Species 0.000 description 2
- 241000607142 Salmonella Species 0.000 description 2
- 241000191967 Staphylococcus aureus Species 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 208000037798 influenza B Diseases 0.000 description 2
- 238000005374 membrane filtration Methods 0.000 description 2
- 238000003908 quality control method Methods 0.000 description 2
- 239000000700 radioactive tracer Substances 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 238000011896 sensitive detection Methods 0.000 description 2
- JJGWLCLUQNFDIS-GTSONSFRSA-M sodium;1-[6-[5-[(3as,4s,6ar)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]hexanoyloxy]-2,5-dioxopyrrolidine-3-sulfonate Chemical compound [Na+].O=C1C(S(=O)(=O)[O-])CC(=O)N1OC(=O)CCCCCNC(=O)CCCC[C@H]1[C@H]2NC(=O)N[C@H]2CS1 JJGWLCLUQNFDIS-GTSONSFRSA-M 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- 108700037148 Brucella BP26 Proteins 0.000 description 1
- 241001148106 Brucella melitensis Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 101710132601 Capsid protein Proteins 0.000 description 1
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 150000001615 biotins Chemical class 0.000 description 1
- 229940038698 brucella melitensis Drugs 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000013098 chemical test method Methods 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 229960005156 digoxin Drugs 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009509 drug development Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000010419 fine particle Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 235000013905 glycine and its sodium salt Nutrition 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 208000037797 influenza A Diseases 0.000 description 1
- 229940117432 influenza b virus antigen Drugs 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000009830 intercalation Methods 0.000 description 1
- 230000002687 intercalation Effects 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 239000013528 metallic particle Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- 238000005325 percolation Methods 0.000 description 1
- 229910052761 rare earth metal Inorganic materials 0.000 description 1
- 150000002910 rare earth metals Chemical class 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Biophysics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510632056.3A CN105403707B (zh) | 2015-09-29 | 2015-09-29 | 一种检测靶物质的信号放大方法及使用该方法的免疫层析试纸和装置 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510632056.3A CN105403707B (zh) | 2015-09-29 | 2015-09-29 | 一种检测靶物质的信号放大方法及使用该方法的免疫层析试纸和装置 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN105403707A true CN105403707A (zh) | 2016-03-16 |
CN105403707B CN105403707B (zh) | 2017-05-17 |
Family
ID=55469316
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201510632056.3A Active CN105403707B (zh) | 2015-09-29 | 2015-09-29 | 一种检测靶物质的信号放大方法及使用该方法的免疫层析试纸和装置 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN105403707B (zh) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107643399A (zh) * | 2017-03-06 | 2018-01-30 | 广州佰芮慷生物科技有限公司 | 一种提高免疫检测系统灵敏性的方法及使用该方法的装置 |
CN109765383A (zh) * | 2019-01-29 | 2019-05-17 | 北京勤邦生物技术有限公司 | 一种猫瘟热病毒抗体荧光检测试纸条及其制备方法和应用 |
CN112666347A (zh) * | 2021-03-17 | 2021-04-16 | 广州敏捷生物技术有限公司 | 同步检测植物油中黄曲霉毒素b1、玉米赤霉烯酮含量的免疫荧光层析试剂盒及方法 |
CN112881672A (zh) * | 2021-01-11 | 2021-06-01 | 南京钟鼎生物技术有限公司 | 通用型多核酸产物检测胶体金试纸条及其制备方法和应用 |
CN116024302A (zh) * | 2022-10-26 | 2023-04-28 | 江苏迅睿生物技术有限公司 | 双联核酸检测层析试纸、引物及探针组、试剂盒 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1500212A (zh) * | 2000-11-30 | 2004-05-26 | ���ס�����˹���� | 有多个被标记部分的信号增强系统 |
US20050095645A1 (en) * | 2002-06-17 | 2005-05-05 | Affymetrix, Inc. | Complexity management of genomic DNA by locus specific amplification |
EP1301627B1 (en) * | 2000-07-07 | 2009-01-21 | Diagnostics for the Real World, Ltd | Improved detection signal and capture in dipstick assays |
CN101865921A (zh) * | 2010-06-11 | 2010-10-20 | 中国检验检疫科学研究院 | 一种检测菜豆荚斑驳病毒的方法及专用试剂盒 |
CN102227631A (zh) * | 2008-11-28 | 2011-10-26 | 英佛皮亚有限公司 | 免疫层析测试中信号放大的方法以及使用该方法的免疫层析试剂盒 |
-
2015
- 2015-09-29 CN CN201510632056.3A patent/CN105403707B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1301627B1 (en) * | 2000-07-07 | 2009-01-21 | Diagnostics for the Real World, Ltd | Improved detection signal and capture in dipstick assays |
CN1500212A (zh) * | 2000-11-30 | 2004-05-26 | ���ס�����˹���� | 有多个被标记部分的信号增强系统 |
US20050095645A1 (en) * | 2002-06-17 | 2005-05-05 | Affymetrix, Inc. | Complexity management of genomic DNA by locus specific amplification |
CN102227631A (zh) * | 2008-11-28 | 2011-10-26 | 英佛皮亚有限公司 | 免疫层析测试中信号放大的方法以及使用该方法的免疫层析试剂盒 |
CN101865921A (zh) * | 2010-06-11 | 2010-10-20 | 中国检验检疫科学研究院 | 一种检测菜豆荚斑驳病毒的方法及专用试剂盒 |
Non-Patent Citations (1)
Title |
---|
曹成: "胶体金核酸层析检测5种检疫性植物病毒的研究", 《中国优秀硕士学位论文全文数据库(农业科技辑)》 * |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107643399A (zh) * | 2017-03-06 | 2018-01-30 | 广州佰芮慷生物科技有限公司 | 一种提高免疫检测系统灵敏性的方法及使用该方法的装置 |
CN109765383A (zh) * | 2019-01-29 | 2019-05-17 | 北京勤邦生物技术有限公司 | 一种猫瘟热病毒抗体荧光检测试纸条及其制备方法和应用 |
CN109765383B (zh) * | 2019-01-29 | 2022-11-18 | 北京勤邦生物技术有限公司 | 一种猫瘟热病毒抗体荧光检测试纸条及其制备方法和应用 |
CN112881672A (zh) * | 2021-01-11 | 2021-06-01 | 南京钟鼎生物技术有限公司 | 通用型多核酸产物检测胶体金试纸条及其制备方法和应用 |
CN112666347A (zh) * | 2021-03-17 | 2021-04-16 | 广州敏捷生物技术有限公司 | 同步检测植物油中黄曲霉毒素b1、玉米赤霉烯酮含量的免疫荧光层析试剂盒及方法 |
CN112666347B (zh) * | 2021-03-17 | 2021-07-06 | 广州敏捷生物技术有限公司 | 同步检测植物油中黄曲霉毒素b1、玉米赤霉烯酮含量的免疫荧光层析试剂盒及方法 |
CN116024302A (zh) * | 2022-10-26 | 2023-04-28 | 江苏迅睿生物技术有限公司 | 双联核酸检测层析试纸、引物及探针组、试剂盒 |
Also Published As
Publication number | Publication date |
---|---|
CN105403707B (zh) | 2017-05-17 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN105403707A (zh) | 一种检测靶物质的信号放大方法及使用该方法的免疫层析试纸和装置 | |
CN101441210B (zh) | 纳米磁性粒子层析试纸检测方法 | |
CN103314297B (zh) | 免疫层析测试条及其制造方法 | |
CN105486854A (zh) | 一种信号放大方法及相关装置 | |
CN101470114B (zh) | 胶体金免疫层析法的增敏检测方法及应用 | |
CN100523814C (zh) | 免疫色谱检测装置 | |
KR20150125002A (ko) | 바이러스 및 박테리아 감염의 복합 검출을 위한 방법 및 장치 | |
CN104076142A (zh) | 微量靶标物质多重联检的荧光微球侧向层析检测条及其制备方法和用途 | |
CN103558381B (zh) | 检测人类艾滋病病毒抗体的免疫层析试纸及其制备方法 | |
CN102236022B (zh) | 一种检测装置 | |
WO2000002049A1 (fr) | Test immunologique et kit de test immunologique | |
CN107643399A (zh) | 一种提高免疫检测系统灵敏性的方法及使用该方法的装置 | |
JP2005291783A (ja) | 免疫測定に供する検体浮遊液調製用媒体組成物及びそれを用いる免疫測定方法 | |
WO2017163341A1 (ja) | イムノクロマト法検査デバイスの試料添加部の形成方法及びイムノクロマト法検査デバイス | |
JP4503334B2 (ja) | 免疫測定に供する検体浮遊液調製用媒体組成物及びそれを用いる免疫測定方法 | |
CN106526166A (zh) | 猪肉中瘦肉精的快速检测 | |
CN103823061A (zh) | Au/Fe3O4免疫层析试纸条及其制备方法与应用 | |
JP7104798B2 (ja) | 免疫クロマトグラフィー用複合粒子及びその製造方法、並びに、免疫クロマトグラフィー | |
JP3841559B2 (ja) | 免疫学的検査方法および免疫学的検査キット | |
US20210389315A1 (en) | Point of care device for early and rapid disease diagnosis | |
CN102388312B (zh) | 分析方法、样品分析用具、防止样品液逆流的方法和防止背景上升的方法 | |
CN108072758A (zh) | 一种检测双酚a的量子点荧光淬灭免疫层析试纸条及其制备方法 | |
JP3930970B2 (ja) | 免疫学的検査方法および免疫学的検査用キット | |
CN109164265A (zh) | 疫苗抗体检测卡及其制备方法 | |
CN104515850A (zh) | 快速测试装置及方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20210519 Address after: 510700 Room 403, building A5, No.3, scientific research road, Science City, Huangpu District, Guangzhou City, Guangdong Province Patentee after: GUANGZHOU BAIRUIKANG BIOTECHNOLOGY Co.,Ltd. Address before: South Medical University, 1838 Guangzhou Avenue North, Guangzhou, Guangdong 510515 Patentee before: SOUTHERN MEDICAL University |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20220706 Address after: Room 301, building a, No. 18, rongshuzhao Industrial Zone, Tongxin community, Baolong street, Longgang District, Shenzhen City, Guangdong Province Patentee after: MIRACLEAN TECHNOLOGY Co.,Ltd. Address before: 510700 Room 403, building A5, No.3, scientific research road, Science City, Huangpu District, Guangzhou City, Guangdong Province Patentee before: GUANGZHOU BAIRUIKANG BIOTECHNOLOGY Co.,Ltd. |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20231031 Address after: Building 6, 201, Yinxing Life and Health Science Park, No. 19 Linhui Road, Jinsha Community, Kengzi Street, Pingshan District, Shenzhen City, Guangdong Province, 518000 Patentee after: Jianguo Biotechnology (Shenzhen) Co.,Ltd. Address before: Room 301, building a, No. 18, rongshuzhao Industrial Zone, Tongxin community, Baolong street, Longgang District, Shenzhen City, Guangdong Province Patentee before: MIRACLEAN TECHNOLOGY Co.,Ltd. |
|
TR01 | Transfer of patent right |