CN105284913A - Pesticide composition containing sophora alopecuroide and vetchleaf sophora root extract as well as preparation method and application thereof - Google Patents
Pesticide composition containing sophora alopecuroide and vetchleaf sophora root extract as well as preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to a pesticide composition containing sophora alopecuroide and vetchleaf sophora root extract as well as a preparation method and application thereof and belongs to the field of pesticides. Aiming at solving the technical problems, the invention provides the pesticide composition containing the sophora alopecuroide and vetchleaf sophora root extract. According to the pesticide composition containing the sophora alopecuroide and vetchleaf sophora root extract, active ingredients are obtained by extracting the following raw medicinal materials in parts by weight: 10-30 parts of sophora alopecuroide and 1 part of vetchleaf sophora root. The common sophora alopecuroide and vetchleaf sophora root extract has a synergistic interaction effect and obvious prevention and treatment effects on crop powdery mildew; the sophora alopecuroide and the vetchleaf sophora root can be commonly extracted so that the content of sophoridine, sophocarpidine and other sterilization active components of which main groups are the same as those of sophoridine and sophocarpidine is improved; the powdery mildew is prevented and treated, the immune system capability of crops can be improved, and the activity can be remarkably improved; Chinese herbal medicines are used as the raw materials so that a pesticide residual problem does not exist; compared with a manner of singly using a chemical source pesticide, the pesticide composition is environmentally friendly; and the effective concentration is low when the pesticide composition is used and the cost is low.
Description
Technical field
The present invention relates to the composition pesticide and its preparation method and application containing Sophora alopecuroide and Sophora viciifolia extract, belong to pesticide field.
Background technology
Sophora alopecuroide is cassia leguminous plant, another name matrine, foxtail-like sophora root, herba sophorae alopecuroide.Sophora alopecuroide is perennial herb, and complete stool is gathered canescence volt kiginu shape pubescence.Owing to being rich in alkaloid in its root, stem, leaf and fruit, mainly contain matrine, oxymatrine, sophocarpine, sophoramine and eulexine, thus Sophora alopecuroide has very high medical value.
Sophora viciifolia, Chinese medicine name; For the root of cassia leguminous plant Sophora viciifolia, leaf, flower, fruit and seed.
Powdery mildew, all can fall ill to heading from seedling; Mainly to cause harm wheat leaf blade, also cause harm stem and tassel; Blade starts produce yellow point, then expand and develop into circle or oval scab, the mould layer of the raw adularescent powdery in surface.Ordinary circumstance bottom blade is more than upper blade, and vacuum side of blade is more than front.Mildew is disperseed separately in early days, after be unified into a large mildew, even can cover full leaf, have a strong impact on photosynthesis, eubolism is interfered, causes early ageing, output incurs loss.
There is not yet in prior art about Sophora alopecuroide and Sophora viciifolia extract for preventing and treating the relevant report in crop powdery mildew.
Summary of the invention
The technical problem that the present invention solves is to provide the composition pesticide containing Sophora alopecuroide and Sophora viciifolia extract.
The present invention contains the composition pesticide of Sophora alopecuroide and Sophora viciifolia extract, and its active component is extracted by the crude drug of following weight portion and forms: Sophora alopecuroide 10 ~ 30 parts, Sophora viciifolia 1 part.
Wherein, its active component is preferably extracted by the crude drug of following weight portion and forms: Sophora alopecuroide 7 ~ 15 parts, Sophora viciifolia 1 part; Preferably extracted by the crude drug of following weight portion and form: Sophora alopecuroide 10 parts, Sophora viciifolia 1 part.
Wherein, its active component jointly to be extracted through organic solution or water by Sophora alopecuroide and Sophora viciifolia and forms; Or the Sophora viciifolia extract that the sophora alopecuroides extract to be obtained to through organic solution or water extraction by Sophora alopecuroide, Sophora viciifolia obtain through organic solution or water extraction mixes; Described organic solvent is acetone, ethyl acetate, ethanol, methyl alcohol or chloroform.
Further, the present invention is microemulsion, soluble concentrate or aqua containing the composition pesticide of Sophora alopecuroide and Sophora viciifolia extract.
Further, the present invention contains in the composition pesticide of Sophora alopecuroide and Sophora viciifolia extract and also comprises auxiliary agent, and described auxiliary agent is at least one in emulsifier, antifreeze, solvent, defoamer, stabilizing agent.
Further, when the described composition pesticide containing Sophora alopecuroide and Sophora viciifolia extract is soluble concentrate, described soluble concentrate is made up of the component of following weight portion: Sophora alopecuroide and Sophora viciifolia extract, 8 parts of dibenzyl xenyl APEOs, 42 parts of ethanol; Wherein, Sophora alopecuroide and Sophora viciifolia extract are that the crude drug of 50 weight portions jointly extracts and forms; Sophora alopecuroide is prepared from by the following method with Sophora viciifolia extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
The present invention contains the composition pesticide of Sophora alopecuroide and Sophora viciifolia extract, and its active component can also be made up of Sophoridine and matrine, and wherein, the mass ratio of Sophoridine and matrine is 1:0.1 ~ 1, is preferably 1:0.4.
Second technical problem that the present invention solves is to provide the preparation method of the composition pesticide containing Sophora alopecuroide and Sophora viciifolia extract.
The present invention contains the preparation method of the composition pesticide of Sophora alopecuroide and Sophora viciifolia extract, it is characterized in that being made up of following steps:
A, extraction: take Sophora alopecuroide particle and Sophora viciifolia particle, mixing, adds solvent by solid-liquid ratio 1:10 ~ 12g/ml, and in 55 ~ 100 DEG C of refluxing extraction, filter, obtain extract, wherein, described solvent is organic solvent or water;
B, preparation: extract is made microemulsion, soluble concentrate or aqua.
Further, in described a step, Sophora alopecuroide particle and Sophora viciifolia particle cross 60 ~ 100 mesh sieves, preferred mistake 80 mesh sieve; Solid-liquid ratio is 1:10g/ml; Organic solvent is methyl alcohol, ethanol, ethyl acetate, acetone, chloroform, is preferably 95%v/v ethanol; Reflux temperature is 75 ~ 85 DEG C, is preferably 82 DEG C; Reflow's cycle is twice, and merging filtrate, obtains extract.
The present invention also provides the application of composition pesticide in control crop powdery mildew containing Sophora alopecuroide and Sophora viciifolia extract; Preferably.
The composition pesticide containing Sophora alopecuroide and Sophora viciifolia extract as the present invention adopts Sophora alopecuroide and Sophora viciifolia extraction to be prepared from, during application, be diluted with water to the activity component concentration of crude drug weighing scale for 10 ~ 100ppm, be more preferably diluted with water to the activity component concentration of crude drug weighing scale as 20 ~ 50ppm.
When the composition pesticide that the present invention contains Sophora alopecuroide and Sophora viciifolia extract adopts Sophoridine and matrine compound as active component, during application, to be diluted with water to active component be concentration is 10 ~ 100ppm, and to be more preferably diluted with water to active component be concentration is 20 ~ 50ppm.
Beneficial effect of the present invention:
1, Sophora alopecuroide of the present invention and the common extract of Sophora viciifolia have synergistic function, obvious to the control efficiency of crop powdery mildew.
2, the present invention adopts Sophora alopecuroide and Sophora viciifolia jointly to extract, improve the content of Sophoridine, matrine and essential groups other fungicide active ingredients identical with it, crop immune system ability can be improved while preventing and treating powdery mildew, and its vigor can be significantly improved.
3, in the present invention, Sophoridine and matrine have synergistic function.
4, the present invention take Chinese herbal medicine as raw material, thus there is not Pesticide Residue; Be used alone compared with chemical source agricultural chemicals, environmentally friendly.
5, the valid density when composition pesticide containing Sophora alopecuroide and Sophora viciifolia extract uses is invented low, with low cost.
Embodiment
The present invention contains the composition pesticide of Sophora alopecuroide and Sophora viciifolia extract, and its active component is extracted by the crude drug of following weight portion and forms: Sophora alopecuroide 10 ~ 30 parts, Sophora viciifolia 1 part.Wherein, preferably formed by the crude drug extraction of following weight portion: Sophora alopecuroide 7 ~ 15 parts, Sophora viciifolia 1 part; Preferably extracted by the crude drug of following weight portion and form: Sophora alopecuroide 10 parts, Sophora viciifolia 1 part.
Wherein, the Sophora alopecuroide that the present invention adopts and Sophora viciifolia are Chinese herbal medicine raw material, contain the activity of the composition pesticide of Sophora alopecuroide and Sophora viciifolia extract in order to improve the present invention, the Sophora alopecuroide that Sophora alopecuroide is preferably dry, the Sophora viciifolia that Sophora viciifolia is preferably dry.
Further, preferably, the present invention jointly to be extracted through organic solution or water by Sophora alopecuroide and Sophora viciifolia containing the active component of the agricultural chemicals of Sophora alopecuroide and Sophora viciifolia extract and forms; Or the Sophora viciifolia extract that the sophora alopecuroides extract to be obtained to through organic solvent or water extraction by Sophora alopecuroide, Sophora viciifolia obtain through organic solvent or water extraction mixes; Described organic solvent is acetone, ethyl acetate, ethanol, methyl alcohol or chloroform.
As further preferred version, described Extraction solvent preferred concentration is 80 ~ 100%v/v ethanol, is more preferably 95%v/v ethanol.
In order to improve the insecticidal properties of composition pesticide of the present invention, after its active component is preferably and is mixed by Sophora alopecuroide and Sophora viciifolia, common extraction forms.
Further, in order to improve the activity of the composition pesticide containing Sophora alopecuroide and Sophora viciifolia extract, be convenient to store and transport, the described composition pesticide containing Sophora alopecuroide and Sophora viciifolia extract can be prepared as the conventional formulations of pesticide, is preferably microemulsion, soluble concentrate or aqua simultaneously.
Further, in order to improve the stability of the composition pesticide containing Sophora alopecuroide and Sophora viciifolia extract, described containing in the composition pesticide of Sophora alopecuroide and Sophora viciifolia extract also comprises auxiliary agent, described auxiliary agent can adopt the usual auxiliaries in composition, as at least one in emulsifier, antifreeze, solvent, stabilizing agent.
Wherein, emulsifier can adopt at least one in non-ionic surface active agent and anion surfactant, described non-ionic surface active agent can be dibenzyl xenyl APEO, styryl phenyl APEO, phenethyl phenyl polyoxyethylene one polyethenoxy ether, OPEO, alkylphenol polyoxyethylene sulfosuccinate, aliphatic acid polyethenoxy ether, fatty alcohol-polyoxyethylene ether, aliphatic amine polyoxyethylene ether, castor oil ethylene oxide adduct and derivative thereof, at least one in polyol fatty acid ester and ethylene oxide adduct etc. thereof, described anion surfactant is at least one in calcium dodecyl benzene sulfonate, alkylphenol polyoxyethylene phosphate, phenethyl phenol polyethenoxy ether phosphate etc., as agriculture breast 500,601p, 602p etc., in addition, other commercially available emulsifier productions can also be used.
Wherein, solvent can adopt at least one in water, ethanol, isopropyl alcohol, n-butanol, ethyl acetate, butyl acetate, methyl-sulfoxide, turpentine oil, solvent naphtha, 1-METHYLPYRROLIDONE, dimethyl formamide etc.
Wherein, antifreeze can be glycerine, propane diols, ethylene glycol, sodium chloride, calcium chloride, sorbierite etc.
Wherein, defoamer is silicone defoaming agent etc.
Wherein, stabilizing agent can be 2-expoxy propane, butyl glycidyl ether, isopropyl alcohol, n-butanol, polyethylene glycol etc.
When the described composition pesticide containing Sophora alopecuroide and Sophora viciifolia extract is soluble concentrate, described soluble concentrate is made up of the component of following weight portion: Sophora alopecuroide and Sophora viciifolia extract, 8 parts of dibenzyl xenyl APEOs, 42 parts of ethanol; Wherein, Sophora alopecuroide and Sophora viciifolia extract are that the crude drug of 50 weight portions jointly extracts and forms; Sophora alopecuroide is prepared from by the following method with Sophora viciifolia extract: crude drug is through extracting and being concentrated into identical with crude drug weight; Wherein, the crude drug of 50 weight portions is Sophora alopecuroide and Sophora viciifolia totally 50 weight portions.
For the ease of storing and transport, extract can be concentrated, obtain concentrate, and then be prepared into various formulation, cycles of concentration can according to needs of production from Row sum-equal matrix, preferably, Sophora alopecuroide is prepared from by the following method with Sophora viciifolia extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
Further, after measured, the present invention has synergistic function containing Sophora alopecuroide and the Sophoridine in the composition pesticide active component of Sophora viciifolia extract, other fungicide active ingredients that matrine is identical with it with essential groups, therefore, the independent extract that the composition pesticide effect prepared by common extract is better than the two mixes the composition pesticide prepared.Meanwhile, through inventor's lot of experiments, find the composition pesticide adopting Sophoridine and matrine binary built to obtain, although activity is a little less than extract, but still there is significant activity.Therefore, from cost-saving angle, the active component that the present invention contains the composition pesticide of Sophora alopecuroide and Sophora viciifolia extract also can be made up of Sophoridine and matrine, and wherein, the mass ratio of Sophoridine and matrine is 1:0.1 ~ 1:1, is more preferably 1:0.4.
Further, the invention also discloses the preparation method that the present invention contains the composition pesticide of Sophora alopecuroide and Sophora viciifolia extract, wherein, according to Sophora alopecuroide and Sophora viciifolia be raw material prepare the composition pesticide of the present invention containing Sophora alopecuroide and Sophora viciifolia extract time, can following methods be adopted:
Method (one):
A, extraction: take Sophora alopecuroide particle and Sophora viciifolia particle, mixing, adds solvent by solid-liquid ratio 1:10 ~ 12g/ml, and in 55 ~ 95 DEG C of refluxing extraction, filter, obtain extract, wherein, described solvent is organic solvent or water;
B, preparation: extract is made microemulsion, soluble concentrate or aqua.
Method (two):
A, extraction: take Sophora alopecuroide particle, add solvent by solid-liquid ratio 1:10 ~ 12g/ml, and in 55 ~ 100 DEG C of refluxing extraction, filter, obtain Sophora alopecuroide extract, wherein, described solvent is organic solvent or water;
Take Sophora viciifolia particle, add solvent by solid-liquid ratio 1:10 ~ 12g/ml, in 55 ~ 100 DEG C of refluxing extraction, filter, obtain Sophora viciifolia extract, wherein, described solvent is organic solvent or water;
B, preparation: by Sophora alopecuroide extract and the mixing of Sophora viciifolia extract, and make microemulsion, soluble concentrate or aqua.
Wherein, in said method () and (two), Sophora alopecuroide and Sophora viciifolia can adopt commercially available particle, conventional method also can be adopted to pulverize Sophora alopecuroide and Sophora viciifolia obtains.In order to improve the effect of extraction, simultaneously cost-saving, Sophora alopecuroide particle and Sophora viciifolia particle cross 60 ~ 100 mesh sieves, preferred mistake 80 mesh sieve.
Further, in order to cost-saving, simultaneously also in order to improve extraction efficiency, the solid-liquid ratio in a step is preferably 1:10g/ml.Namely gross weight is that the Sophora alopecuroide particle of lg and Sophora viciifolia particle add 10 ~ 12ml solvent extraction, preferably adds 10ml solvent extraction.
Conventional organic solvent all can be used for the present invention, and in order to cost-saving, improve dissolution rate, ensure extraction efficiency, organic solvent is preferably methyl alcohol, ethanol, ethyl acetate, acetone, chloroform simultaneously, is more preferably the ethanol that volume fraction is 95%.
In order to shorten preparation time, accelerate extraction rate, can stir when adding hot reflux, mixing speed is preferably 80 ~ 100 revs/min.
Further, reflux temperature is preferably 75 ~ 85 DEG C, is more preferably 82 DEG C; Reflow's cycle is twice, and each return time is 1.5 hours, and merging filtrate obtains corresponding extract.
Further, the invention also discloses the application of composition pesticide in control crop powdery mildew containing Sophora alopecuroide and Sophora viciifolia extract.
In the agricultural production of reality, in order to improve composition pesticide containing Sophora alopecuroide and Sophora viciifolia extract to the control efficiency of powdery mildew, the described composition pesticide containing Sophora alopecuroide and Sophora viciifolia extract is preferably applied to crop powdery mildew initial stage of origination; The present invention can use by common method containing the composition pesticide of Sophora alopecuroide and Sophora viciifolia extract, sprays on crop.Preferably, the composition pesticide containing Sophora alopecuroide and Sophora viciifolia extract as the present invention adopts Sophora alopecuroide and Sophora viciifolia extraction to be prepared from, be diluted with water to when then using with the activity component concentration of crude drug weighing scale as 10 ~ 100ppm, namely the composition pesticide that 1g crude drug is obtained after extracting, when using, be diluted with water to 10kg, then its activity component concentration equals 100ppm; Preferably being diluted to further with the activity component concentration of crude drug weighing scale is that 20 ~ 50ppm sprays in plant blade face.When the composition pesticide that the present invention contains Sophora alopecuroide and Sophora viciifolia extract adopts Sophoridine and matrine compound as active component, being then diluted with water to activity component concentration in composition pesticide when using is 10 ~ 100ppm; Preferably being diluted to activity component concentration is further that 20 ~ 50ppm sprays in plant blade face.When spraying, so that water membrane to appear in blade face, not lower of liquid is advisable; Secondary dispenser is carried out, the preferred continuous administration of a season crop 2 ~ 3 times after being preferable over 3 ~ 5 days.
Below in conjunction with embodiment, the specific embodiment of the present invention is further described, does not therefore limit the present invention among described scope of embodiments.
Embodiment 1 the present invention contains the preparation of the composition pesticide of Sophora alopecuroide and Sophora viciifolia extract
A, extraction: take Sophora alopecuroide, removing impurity, cleans, slightly moistens, drying, takes Sophora viciifolia, and removing impurity, cleans, run through, dry, by Sophora alopecuroide and Sophora viciifolia mixing, pulverize, cross 80 mesh sieves, add solvent, refluxing extraction twice, filters, merging filtrate, concentrated, make extract weight equal the weight of crude drug, obtain extract bq-01 ~ bq-06; Wherein, each parameter is in table 1;
B, preparation: in extract bq-01 ~ bq-06, add auxiliary agent in table 2, stir, must contain the composition pesticide BQ-01 ~ BQ-06 of Sophora alopecuroide and Sophora viciifolia extract.
Table 1
Wherein, the Sophora alopecuroide used in the present invention and Sophora viciifolia are all purchased from Anguo City Zhong Tian prepared slices of Chinese crude drugs Co., Ltd.
Table 2
Embodiment 2 the present invention contains the preparation of the composition pesticide of Sophora alopecuroide and Sophora viciifolia extract
A, extraction: take Sophora alopecuroide, removing impurity, cleans, slightly moistens, dry, pulverize, cross 80 mesh sieves, add solvent, refluxing extraction twice, filter, merging filtrate, concentrated, make extract weight equal the weight of crude drug, obtain Sophora alopecuroide extract d1-01 ~ d1-03, wherein, each parameter is in table 3;
Take Sophora viciifolia, removing impurity, cleans, runs through, dry, pulverize, cross 80 mesh sieves, add solvent, refluxing extraction twice, filter, merging filtrate, concentrated, make extract weight equal the weight of crude drug, obtain Sophora viciifolia extract d2-01 ~ d2-03, wherein, each parameter is in table 4;
B, preparation: take Sophora alopecuroide extract, Sophora viciifolia extract and auxiliary agent, stirring and evenly mixing, obtain the composition pesticide BQ-07 ~ BQ-12 containing Sophora alopecuroide and Sophora viciifolia extract, wherein, each parameter is in table 5.
Table 3
Table 4
Table 5
Embodiment 3 the present invention contains the preparation of the composition pesticide of Sophora alopecuroide and Sophora viciifolia extract
Take Sophoridine and matrine and auxiliary agent according to proportioning in table 6, mixing, stir, stirring and evenly mixing, obtains the composition pesticide BQ-13 ~ BQ-18 containing Sophora alopecuroide and Sophora viciifolia extract.
Table 6
Wherein, Sophoridine and matrine are all purchased from Ze Lang bio tech ltd, Nanjing.
Test example 1 the present invention contains the composition pesticide powdery mildew of strawberry efficiency test of Sophora alopecuroide and Sophora viciifolia extract
1 test material
1.1 for studying thing and object: powdery mildew of strawberry
1.2 medicament
Test medicine: BQ-01 ~ BQ-18;
Sophora alopecuroide single dose (D1); Sophoridine single dose (D2); Sophora viciifolia single dose (D3); Single matrine (D4);
Clear water contrast (CK).
Wherein, the preparation method of Sophora alopecuroide single dose is: the d1-03 getting 50g, and add 8g dibenzyl xenyl APEO, 42g ethanol, stirring and evenly mixing, to obtain final product.Sophora viciifolia single dose preparation method is: the d2-03 getting 50g, and add 8g dibenzyl xenyl APEO, 42g ethanol, stirring and evenly mixing, to obtain final product.The preparation method of Sophoridine single dose is: the Sophoridine getting 50g, and add 8g dibenzyl xenyl APEO, 42g ethanol, stirring and evenly mixing, to obtain final product.Single matrine preparation method is: the matrine getting 50g, and add 8g dibenzyl xenyl APEO, 42g ethanol, stirring and evenly mixing, to obtain final product.
2, experimental scheme and method
Small plot experiment is designed with reference to standard GB/T/T17980.119-2004 " field control effectiveness test criterion (two) bactericidal agent for preventing and treating powdery mildew of strawberry ".
This test 18 medicaments, 4 single dose contrasts, 1 blank, repeats for three times, Gong69Ge community.Minizone and surrounding all leave isolation strip, plot area 12m
2, community respectively processes randomized arrangement.
By medicament BQ-01 ~ BQ-12, D1, D3 thin up to the activity component concentration of crude drug weighing scale for 50ppm; Be 50ppm by BQ-13 ~ BQ-18, D2, D4 thin up to activity component concentration; And spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Test adopts following stage division:
0 grade: anosis;
1 grade: lesion area accounts for less than 5% of whole leaf area;
3 grades: lesion area accounts for the 6%-15% of whole leaf area;
5 grades: lesion area accounts for the 16%-25% of whole leaf area;
7 grades: lesion area accounts for the 26%-50% of whole leaf area;
9 grades: lesion area accounts for more than 50% of whole leaf area.
According to survey data, calculate disease index and the control efficiency of each process.
Disease index is pressed formula (1) and is calculated, and result of calculation retains 2 significant digits:
Control efficiency is pressed formula (2) and is calculated, and result of calculation retains 2 significant digits:
3, result of the test
According to total strain number of 14 days and diseased plant number before above-mentioned stage division and the dispenser of data processing formula statistics and after dispenser, calculate disease index and control efficiency, the results are shown in Table 7.
Table 7
| Reagent agent | Disease index before medicine | Disease index after medicine | Control efficiency (%) |
| BQ-01 | 19.2 | 15.8 | 77.70 |
| BQ-02 | 19.6 | 13.6 | 81.20 |
| BQ-03 | 17.8 | 14.2 | 78.38 |
| BQ-04 | 18.4 | 11.7 | 82.77 |
| BQ-05 | 19.2 | 14.8 | 79.11 |
| BQ-06 | 16.5 | 13.9 | 77.17 |
| BQ-07 | 15.9 | 22.2 | 62.17 |
| BQ-08 | 17.3 | 20.1 | 68.52 |
| BQ-09 | 22.1 | 23.6 | 71.07 |
| BQ-10 | 20.5 | 19.8 | 73.83 |
| BQ-11 | 19.9 | 18.9 | 74.27 |
| BQ-12 | 18.7 | 19.5 | 71.75 |
| BQ-13 | 19.1 | 23.5 | 66.66 |
| BQ-14 | 19.6 | 24.1 | 66.68 |
| BQ-15 | 18.5 | 23.2 | 66.02 |
| BQ-16 | 19.2 | 21.7 | 69.38 |
| BQ-17 | 19.9 | 22.9 | 68.82 |
| BQ-18 | 20.1 | 21.1 | 71.56 |
| D1 | 17.9 | 34.2 | 48.23 |
| D2 | 18.3 | 31.2 | 53.81 |
| D3 | 17.6 | 35.6 | 45.19 |
| D4 | 18.8 | 31.6 | 54.46 |
| CK | 19.4 | 71.6 | -- |
As shown in Table 7, the present invention has good preventive effect containing the composition pesticide of Sophora alopecuroide and Sophora viciifolia extract to powdery mildew of strawberry.
The preventive effect of the composition pesticide (BQ-01 ~ BQ-06) adopting common extracting method to make is apparently higher than the composition pesticide (BQ-07 ~ BQ-012) be mixed to get after extracting separately, visible common extraction contributes to improving dissolution rate, improves germicidal efficiency; And the control efficiency of Sophora viciifolia single dose D3 that the Sophora alopecuroide single dose D1 be prepared from than single sophora alopecuroides extract and Sophora viciifolia extract are prepared from is good, visible, has synergistic function between product compositions of the present invention.
Meanwhile, the preventive effect of the composition pesticide (BQ-13 ~ BQ-18) adopting Sophoridine and matrine compound to obtain is also apparently higher than Sophoridine single dose D2 and single matrine D4, and visible, Sophoridine and matrine compound have synergistic function.
In addition, have no during dispenser and have poisoning to show to plant.
Claims (10)
1. contain the composition pesticide of Sophora alopecuroide and Sophora viciifolia extract, it is characterized in that, its active component is extracted by the crude drug of following weight portion and forms: Sophora alopecuroide 10 ~ 30 parts, Sophora viciifolia 1 part.
2. the composition pesticide containing Sophora alopecuroide and Sophora viciifolia extract according to claim 1, its active component is extracted by the crude drug of following weight portion and forms: Sophora alopecuroide 7 ~ 15 parts, Sophora viciifolia 1 part; Preferably extracted by the crude drug of following weight portion and form: Sophora alopecuroide 10 parts, Sophora viciifolia 1 part.
3. the composition pesticide of Sophora alopecuroide according to claim 1 and 2 and Sophora viciifolia extract, is characterized in that: its active component jointly to be extracted through organic solution or water by Sophora alopecuroide and Sophora viciifolia and forms; Or the Sophora viciifolia extract that the sophora alopecuroides extract to be obtained to through organic solution or water extraction by Sophora alopecuroide, Sophora viciifolia obtain through organic solution or water extraction mixes; Described organic solvent is acetone, ethyl acetate, ethanol, methyl alcohol or chloroform.
4. the composition pesticide containing Sophora alopecuroide and Sophora viciifolia extract according to any one of claims 1 to 3, is characterized in that: the described composition pesticide containing Sophora alopecuroide and Sophora viciifolia extract is microemulsion, soluble concentrate or aqua.
5. the composition pesticide containing Sophora alopecuroide and Sophora viciifolia extract according to any one of Claims 1 to 4, it is characterized in that: described containing in the composition pesticide of Sophora alopecuroide and Sophora viciifolia extract also comprises auxiliary agent, and described auxiliary agent is at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
6. the composition pesticide containing Sophora alopecuroide and Sophora viciifolia extract according to claim 5, it is characterized in that, when the described composition pesticide containing Sophora alopecuroide and Sophora viciifolia extract is soluble concentrate, described soluble concentrate is made up of the component of following weight portion: Sophora alopecuroide and Sophora viciifolia extract, 8 parts of dibenzyl xenyl APEOs, 42 parts of ethanol; Wherein, Sophora alopecuroide and Sophora viciifolia extract are that the crude drug of 50 weight portions jointly extracts and forms; Sophora alopecuroide is prepared from by the following method with Sophora viciifolia extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
7. contain the composition pesticide of Sophora alopecuroide and Sophora viciifolia extract, it is characterized in that: its active component is made up of Sophoridine and matrine, wherein, the mass ratio of Sophoridine and matrine is 1:0.1 ~ 1, is preferably 1:0.4.
8. the preparation method containing the composition pesticide of Sophora alopecuroide and Sophora viciifolia extract described in any one of claim 1 ~ 6, is characterized in that being made up of following steps:
A, extraction: take Sophora alopecuroide particle and Sophora viciifolia particle, mixing, adds solvent by solid-liquid ratio 1:10 ~ 12g/ml, and in 55 ~ 100 DEG C of refluxing extraction, filter, obtain extract, wherein, described solvent is organic solvent or water;
B, preparation: extract is made microemulsion, soluble concentrate or aqua.
9. the preparation method containing the composition pesticide of Sophora alopecuroide and Sophora viciifolia extract according to claim 8, it is characterized in that: in described a step, Sophora alopecuroide particle and Sophora viciifolia particle cross 60 ~ 100 mesh sieves, preferred mistake 80 mesh sieve; Solid-liquid ratio is 1:10g/ml; Organic solvent is methyl alcohol, ethanol, ethyl acetate, acetone, chloroform, is preferably 95%v/v ethanol; Reflux temperature is 75 ~ 85 DEG C, is preferably 82 DEG C; Reflow's cycle is twice, and merging filtrate, obtains extract.
10. the composition pesticide containing Sophora alopecuroide and Sophora viciifolia extract described in any one of claim 1 ~ 6 is preventing and treating the application in crop powdery mildew; Further, during application, be diluted with water to the activity component concentration of crude drug weighing scale for 10 ~ 100ppm, be more preferably diluted with water to the activity component concentration of crude drug weighing scale as 20 ~ 50ppm; Or
The described composition pesticide containing Sophora alopecuroide and Sophora viciifolia extract according to claim 7 is preventing and treating the application in crop powdery mildew; Further, during application, to be diluted with water to active component be concentration is 10 ~ 100ppm, and to be more preferably diluted with water to active component be concentration is 20 ~ 50ppm.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106259539A (en) * | 2016-08-16 | 2017-01-04 | 惠安县科联农业科技有限公司 | A kind of biological control method of powdery mildew of strawberry |
| CN108558499A (en) * | 2018-04-09 | 2018-09-21 | 山东省农业科学院农业资源与环境研究所 | A kind of compound formulation for melon crop of the extract containing edible fungi residue |
| CN113170798A (en) * | 2021-03-29 | 2021-07-27 | 贵州省山地资源研究所 | Composite resistance inducer for preventing and treating early alternaria leaf spot of red-yang kiwi fruits in southern areas as well as preparation method and application of composite resistance inducer |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104082357A (en) * | 2014-06-27 | 2014-10-08 | 何芳 | Botanical biopesticide |
-
2015
- 2015-12-04 CN CN201510884984.9A patent/CN105284913A/en not_active Withdrawn
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104082357A (en) * | 2014-06-27 | 2014-10-08 | 何芳 | Botanical biopesticide |
Non-Patent Citations (2)
| Title |
|---|
| 张沙沙: ""苦豆子根提取物杀菌制剂研究"", 《中国优秀硕士学位论文全文数据库农业科技辑》 * |
| 陈青: "中国优秀硕士学位论文全文数据库农业科技辑", 《中国优秀硕士学位论文全文数据库医药卫生科技辑》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106259539A (en) * | 2016-08-16 | 2017-01-04 | 惠安县科联农业科技有限公司 | A kind of biological control method of powdery mildew of strawberry |
| CN108558499A (en) * | 2018-04-09 | 2018-09-21 | 山东省农业科学院农业资源与环境研究所 | A kind of compound formulation for melon crop of the extract containing edible fungi residue |
| CN113170798A (en) * | 2021-03-29 | 2021-07-27 | 贵州省山地资源研究所 | Composite resistance inducer for preventing and treating early alternaria leaf spot of red-yang kiwi fruits in southern areas as well as preparation method and application of composite resistance inducer |
| CN113170798B (en) * | 2021-03-29 | 2022-04-15 | 贵州省山地资源研究所 | Composite resistance inducer for preventing and treating early alternaria leaf spot of red-yang kiwi fruits in southern areas as well as preparation method and application of composite resistance inducer |
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Address after: 611630 No. five, No. 35 Industrial Road, Heshan Town, Pujiang County, Sichuan, Chengdu Applicant after: Chengdu new Chaoyang Crop Science Co., Ltd Address before: 611630 No. five, No. 35 Industrial Road, Heshan Town, Pujiang County, Sichuan, Chengdu Applicant before: CHENGDU NEWSUN CROPSCIENCE Co.,Ltd. |
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Application publication date: 20160203 |