CN105340986A - Pesticide composition containing mint and schizonepeta extract as well as preparation method and application of pesticide composition - Google Patents
Pesticide composition containing mint and schizonepeta extract as well as preparation method and application of pesticide composition Download PDFInfo
- Publication number
- CN105340986A CN105340986A CN201510882977.5A CN201510882977A CN105340986A CN 105340986 A CN105340986 A CN 105340986A CN 201510882977 A CN201510882977 A CN 201510882977A CN 105340986 A CN105340986 A CN 105340986A
- Authority
- CN
- China
- Prior art keywords
- peppermint
- extract
- herba schizonepetae
- schizonepeta
- composition pesticide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/06—Oxygen or sulfur directly attached to a cycloaliphatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/06—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing keto or thioketo groups as part of a ring, e.g. cyclohexanone, quinone; Derivatives thereof, e.g. ketals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Pest Control & Pesticides (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a pesticide composition containing a mint and schizonepeta extract as well as a preparation method and application of the pesticide composition, belongs to the field of pesticide, and solves the technical problem of providing the pesticide composition containing the mint and schizonepeta extract. The active components of the pesticide composition containing the mint and schizonepeta extract are extracted from the following raw medicinal materials in parts by weight: 10-30 parts of mint and 1 part of schizonepeta. The common extract of mint and schizonepeta has a synergistic effect, and has an obvious prevention and control effect on the apple canker of crops; mint and schizonepeta can be extracted together, and the contents of menthol, dextrorotatory menthone, and other bactericidal active components the same as mint and schizonepeta in main perssad are increased, so that the immune system function of crops can be improved while the apple canker is prevented and controlled, and the activity of crops can be remarkably improved; Chinese herbal medicine can be adopted as the raw materials, so that the pesticide residue problem is avoided; compared with separately used chemical source pesticide, the pesticide composition has the advantages of environment friendliness, low effective concentration in use and low cost.
Description
Technical field
The present invention relates to the composition pesticide and its preparation method and application containing peppermint and Herba Schizonepetae extract, belong to pesticide field.
Background technology
Schizonepeta is labiate, and its herb or its leaf, stem etc. can extract volatile oil; This volatile oil is rich in menthones and pulegone, and both account for the 60-80% of volatile oil usually.Because Herba Schizonepetae volatile oil or extract main component menthones and pulegone have antibacterial and effect that is expelling parasite, thus before it mainly as the people of antibacterial and expelling parasite with or natural drug for animals.
Although Herba Schizonepetae volatile oil or extract can be used as botanical pesticide and product, it is limited that its anti-impact effect very generally makes it apply.
At present, this area is when improving the insect resistant effect of botanical pesticide, the mode adopted generally has two kinds: one to be carry out chemical modification to it, obtain the derivative of better effects if, as the patent 200580045099/200380015280 and 200810129703 etc. of E.I.Du Pont Company, hydrogenated derivatives is wherein mainly used in expelling parasite; Two is apply with other agriculture chemical compoundings, promotes insect resistant effect, if application number is 201410433059X, 201410237373 etc.
But when prior art to there is not yet about peppermint and Herba Schizonepetae extract as Pesticide use, there is the relevant report of synergistic function.
Summary of the invention
The technical problem that the present invention solves is to provide the composition pesticide containing peppermint and Herba Schizonepetae extract.
The present invention contains the composition pesticide of peppermint and Herba Schizonepetae extract, and its active component is extracted by the crude drug of following weight portion and forms: peppermint 10 ~ 30 parts, schizonepeta 1 part.
Wherein, its active component is preferably extracted by the crude drug of following weight portion and forms: peppermint 15 ~ 25 parts, schizonepeta 1 part; More preferably extracted by the crude drug of following weight portion and form: peppermint 22 parts, schizonepeta 1 part.
Wherein, its active component jointly to be extracted through organic solution or water by peppermint and schizonepeta and forms; Or the Herba Schizonepetae extract that the mint extract to be obtained to through organic solution or water extraction by peppermint, schizonepeta obtain through organic solution or water extraction mixes; Described organic solvent is acetone, ethyl acetate, ethanol, methyl alcohol or chloroform; Be preferably ethanol or methyl alcohol.
Further, the present invention is microemulsion, soluble concentrate or missible oil containing the composition pesticide of peppermint and Herba Schizonepetae extract.
Further, the present invention contains in the composition pesticide of peppermint and Herba Schizonepetae extract and also comprises auxiliary agent, and described auxiliary agent is at least one in emulsifier, antifreeze, solvent, defoamer, stabilizing agent.
Further, when the described composition pesticide containing peppermint and Herba Schizonepetae extract makes soluble concentrate,
Described soluble concentrate is made up of the component of following weight portion: peppermint and Herba Schizonepetae extract, dibenzyl xenyl APEO 6 parts, ethanol 44 parts; Wherein, peppermint and Herba Schizonepetae extract are that the crude drug of 50 weight portions jointly extracts and forms; Peppermint is prepared from by the following method with Herba Schizonepetae extract: crude drug is through extracting and being concentrated into identical with crude drug weight;
Or: described soluble concentrate is made up of the component of following weight portion: peppermint and Herba Schizonepetae extract, 10g castor oil ethylene oxide condensate, 30g ethanol; Wherein, peppermint and Herba Schizonepetae extract are that the crude drug of 60 weight portions jointly extracts and forms; Peppermint is prepared from by the following method with Herba Schizonepetae extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
The present invention contains the composition pesticide of peppermint and Herba Schizonepetae extract, and its active component can also be made up of menthol and D-menthone, and wherein, the mass ratio of menthol and D-menthone is 1:0.1 ~ 1, is preferably 1:0.4.
Second technical problem that the present invention solves is to provide the preparation method of the composition pesticide containing peppermint and Herba Schizonepetae extract.
The present invention contains the preparation method of the composition pesticide of peppermint and Herba Schizonepetae extract, it is characterized in that being made up of following steps:
A, extraction: take mint particles and schizonepeta particle, mixing, adds solvent by solid-liquid ratio 1:8 ~ 12g/ml, and in 55 ~ 100 DEG C of refluxing extraction, filter, obtain extract, wherein, described solvent is organic solvent or water;
B, preparation: extract is made microemulsion, soluble concentrate or missible oil.
Further, in described a step, mint particles and schizonepeta particle cross 60 ~ 100 mesh sieves, preferred mistake 80 mesh sieve; Solid-liquid ratio is 1:10 ~ 12g/ml; Organic solvent is methyl alcohol, ethanol, ethyl acetate, acetone, chloroform, is preferably 95%v/v ethanol or acetone; Reflux temperature is 65 ~ 85 DEG C; Reflow's cycle is twice, and merging filtrate, obtains extract.
The present invention also provides the application of composition pesticide in control crops apples rot containing peppermint and Herba Schizonepetae extract.
The composition pesticide containing peppermint and Herba Schizonepetae extract as the present invention adopts peppermint and schizonepeta extraction to be prepared from, during application, be diluted with water to the activity component concentration of crude drug weighing scale for 10 ~ 100ppm, be more preferably diluted with water to the activity component concentration of crude drug weighing scale as 20 ~ 50ppm.
When the present invention containing the composition pesticide of peppermint and Herba Schizonepetae extract adopt menthol and D-menthone composite as active component time, during application, to be diluted with water to active component be concentration is 10 ~ 100ppm, and to be more preferably diluted with water to active component be concentration is 20 ~ 50ppm.
Beneficial effect of the present invention:
1, peppermint of the present invention and the common extract of schizonepeta have synergistic function, obvious to the control efficiency of crops apples rot.
2, the present invention adopts peppermint and schizonepeta jointly to extract, improve the content of menthol, D-menthone and essential groups other fungicide active ingredients identical with it, crop immune system ability can be improved while preventing and treating apple canker, and its vigor can be significantly improved.
3, in the present invention, menthol and D-menthone have synergistic function.
4, the present invention take Chinese herbal medicine as raw material, thus there is not Pesticide Residue; Be used alone compared with chemical source agricultural chemicals, environmentally friendly.
5, the valid density when composition pesticide containing peppermint and Herba Schizonepetae extract uses is invented low, with low cost.
Embodiment
The present invention contains the composition pesticide of peppermint and Herba Schizonepetae extract, and its active component is extracted by the crude drug of following weight portion and forms: peppermint 10 ~ 30 parts, schizonepeta 1 part.Wherein, preferably formed by the crude drug extraction of following weight portion: peppermint 15 ~ 25 parts, schizonepeta 1 part; More preferably extracted by the crude drug of following weight portion and form: peppermint 22 parts, schizonepeta 1 part.
Wherein, the peppermint that the present invention adopts and schizonepeta are Chinese herbal medicine raw material, contain the activity of the composition pesticide of peppermint and Herba Schizonepetae extract in order to improve the present invention, the peppermint that peppermint is preferably dry, the schizonepeta that schizonepeta is preferably dry.
Further, preferably, the present invention jointly to be extracted through organic solution or water by peppermint and schizonepeta containing the active component of the agricultural chemicals of peppermint and Herba Schizonepetae extract and forms; Or the Herba Schizonepetae extract that the mint extract to be obtained to through organic solvent or water extraction by peppermint, schizonepeta obtain through organic solvent or water extraction mixes; Described organic solvent is acetone, ethyl acetate, ethanol, methyl alcohol or chloroform.
As further preferred version, described Extraction solvent preferred concentration is 80 ~ 100%v/v ethanol, is more preferably 95%v/v ethanol.
In order to improve the insecticidal properties of composition pesticide of the present invention, after its active component is preferably and is mixed by peppermint and schizonepeta, common extraction forms.
Further, in order to improve the activity of the composition pesticide containing peppermint and Herba Schizonepetae extract, be convenient to store and transport, the described composition pesticide containing peppermint and Herba Schizonepetae extract can be prepared as the conventional formulations of pesticide, is preferably microemulsion, soluble concentrate or missible oil simultaneously.
Further, in order to improve the stability of the composition pesticide containing peppermint and Herba Schizonepetae extract, described containing in the composition pesticide of peppermint and Herba Schizonepetae extract also comprises auxiliary agent, described auxiliary agent can adopt the usual auxiliaries in composition, as at least one in emulsifier, antifreeze, solvent, defoamer, stabilizing agent.
Wherein, emulsifier can adopt at least one in non-ionic surface active agent and anion surfactant, and described non-ionic surface active agent can be at least one in dibenzyl xenyl APEO, styryl phenyl APEO, phenethyl phenyl polyoxyethylene one polyethenoxy ether, alkylphenol polyoxyethylene, alkylphenol polyoxyethylene sulfosuccinate, fatty alcohol-polyoxyethylene ether, aliphatic amine polyoxyethylene ether, castor oil ethylene oxide adduct and derivative, polyol fatty acid ester and ethylene oxide adduct thereof etc.; Described anion surfactant is at least one in calcium dodecyl benzene sulfonate, alkylphenol polyoxyethylene phosphate, phenethyl phenol polyethenoxy ether phosphate etc., as agriculture breast 500,601p, 602p etc.; In addition, other commercially available emulsifier productions can also be used.
Wherein, solvent can adopt at least one in water, ethanol, isopropyl alcohol, ethyl acetate, butyl acetate, methyl-sulfoxide, turpentine oil, solvent naphtha, 1-METHYLPYRROLIDONE, dimethyl formamide etc.
Wherein, antifreeze can be glycerine, propane diols, ethylene glycol etc.Wherein, defoamer is silicone defoaming agent etc.
For the ease of storing and transport, extract can be concentrated, obtain concentrate, and then be prepared into various formulation, cycles of concentration can according to needs of production from Row sum-equal matrix, preferably, peppermint is prepared from by the following method with Herba Schizonepetae extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
Further, after measured, the present invention has synergistic function containing peppermint and the menthol in the composition pesticide active component of Herba Schizonepetae extract, other fungicide active ingredients that D-menthone is identical with it with essential groups, therefore, the independent extract that the composition pesticide effect prepared by common extract is better than the two mixes the composition pesticide prepared.Meanwhile, through inventor's lot of experiments, find the composition pesticide adopting menthol and D-menthone binary built to obtain, although activity is a little less than extract, but still there is significant activity.Therefore, from cost-saving angle, the active component that the present invention contains the composition pesticide of peppermint and Herba Schizonepetae extract also can be made up of menthol and D-menthone, wherein, the mass ratio of menthol and D-menthone is 1:0.1 ~ 1:1, is more preferably 1:0.4.
Further, the invention also discloses the preparation method of the present invention containing the composition pesticide of peppermint and Herba Schizonepetae extract, wherein, according to peppermint and schizonepeta be raw material prepare the composition pesticide of the present invention containing peppermint and Herba Schizonepetae extract time, can following methods be adopted:
Method (one):
A, extraction: take mint particles and schizonepeta particle, mixing, adds solvent by solid-liquid ratio 1:8 ~ 12g/ml, and in 55 ~ 100 DEG C of refluxing extraction, filter, obtain extract, wherein, described solvent is organic solvent or water;
B, preparation: extract is made microemulsion, soluble concentrate or aqua.
Method (two):
A, extraction: take mint particles, add solvent by solid-liquid ratio 1:8 ~ 12g/ml, and in 55 ~ 100 DEG C of refluxing extraction, filter, obtain peppermint extract, wherein, described solvent is organic solvent or water; Take schizonepeta particle, add solvent by solid-liquid ratio 1:8 ~ 12g/ml, in 55 ~ 100 DEG C of refluxing extraction, filter, obtain schizonepeta extract, wherein, described solvent is organic solvent or water;
B, preparation: by peppermint extract and the mixing of schizonepeta extract, and make microemulsion, soluble concentrate or missible oil.
Wherein, in said method () and (two), peppermint and schizonepeta can adopt commercially available particle, conventional method also can be adopted to pulverize peppermint and schizonepeta obtains.In order to improve the effect of extraction, simultaneously cost-saving, mint particles and schizonepeta particle cross 60 ~ 100 mesh sieves, preferred mistake 80 mesh sieve.
Further, in order to cost-saving, simultaneously also in order to improve extraction efficiency, the solid-liquid ratio in a step is preferably 1:10 ~ 12g/ml.Namely gross weight is that the mint particles of lg and schizonepeta particle add 8 ~ 12ml solvent extraction.
Conventional organic solvent all can be used for the present invention, and in order to cost-saving, improve dissolution rate, ensure extraction efficiency, organic solvent is preferably methyl alcohol, ethanol, ethyl acetate, acetone, chloroform simultaneously, is more preferably ethanol or methyl alcohol that volume fraction is 95%.
In order to shorten preparation time, accelerate extraction rate, can stir when adding hot reflux, mixing speed is preferably 80 ~ 100 revs/min.
Further, reflux temperature is preferably 65 ~ 85 DEG C; Reflow's cycle is twice, and each return time is 2 hours, and merging filtrate obtains corresponding extract.
Further, the invention also discloses the application of composition pesticide in control crops apples rot containing peppermint and Herba Schizonepetae extract.
In the agricultural production of reality, in order to improve composition pesticide containing peppermint and Herba Schizonepetae extract to the control efficiency of apple canker, the described composition pesticide containing peppermint and Herba Schizonepetae extract is preferably applied to crops apples rot initial stage of origination; The present invention can use by common method containing the composition pesticide of peppermint and Herba Schizonepetae extract, sprays on crop.Preferably, the composition pesticide containing peppermint and Herba Schizonepetae extract as the present invention adopts peppermint and schizonepeta extraction to be prepared from, be diluted with water to when then using with the activity component concentration of crude drug weighing scale as 10 ~ 100ppm, namely the composition pesticide that 1g crude drug is obtained after extracting, when using, be diluted with water to 10kg, then its activity component concentration equals 100ppm; Preferably being diluted to further with the activity component concentration of crude drug weighing scale is that 20 ~ 50ppm sprays in plant blade face.When the present invention containing the composition pesticide of peppermint and Herba Schizonepetae extract adopt menthol and D-menthone composite as active component time, being then diluted with water to activity component concentration in composition pesticide when using is 10 ~ 100ppm; Preferably being diluted to activity component concentration is further that 20 ~ 50ppm sprays in plant blade face.When spraying, so that water membrane to appear in blade face, not lower of liquid is advisable; Secondary dispenser is carried out, the preferred continuous administration of a season crop 2 ~ 3 times after being preferable over 3 ~ 5 days.
Below in conjunction with embodiment, the specific embodiment of the present invention is further described, does not therefore limit the present invention among described scope of embodiments.
Embodiment 1 the present invention contains the preparation of the composition pesticide of peppermint and Herba Schizonepetae extract
A, extraction: take peppermint, removing impurity, cleans, slightly moistens, drying, takes schizonepeta, and removing impurity, cleans, run through, cut sheet or section, dry, by peppermint and schizonepeta mixing, pulverize, cross 80 mesh sieves, add solvent, refluxing extraction twice, filters, merging filtrate, concentrated, make extract weight equal the weight of crude drug, obtain extract bq-01 ~ bq-06; Wherein, each parameter is in table 1;
B, preparation: in extract bq-01 ~ bq-06, add auxiliary agent in table 2, stir, must contain the composition pesticide BQ-01 ~ BQ-06 of peppermint and Herba Schizonepetae extract.
Table 1
Wherein, the peppermint used in the present invention and schizonepeta are all purchased from Anguo City Zhong Tian prepared slices of Chinese crude drugs Co., Ltd.
Table 2
Embodiment 2 the present invention contains the preparation of the composition pesticide of peppermint and Herba Schizonepetae extract
A, extraction: take peppermint, removing impurity, cleans, slightly moistens, dry, pulverize, cross 80 mesh sieves, add solvent, refluxing extraction twice, filter, merging filtrate, concentrated, make extract weight equal the weight of crude drug, obtain peppermint extract d1-01 ~ d1-03, wherein, each parameter is in table 3;
Take schizonepeta, removing impurity, cleans, runs through, dry, pulverize, cross 80 mesh sieves, add solvent, refluxing extraction twice, filter, merging filtrate, concentrated, make extract weight equal the weight of crude drug, obtain schizonepeta extract d2-01 ~ d2-03, wherein, each parameter is in table 4;
B, preparation: take peppermint extract, schizonepeta extract and auxiliary agent, stirring and evenly mixing, obtain the composition pesticide BQ-07 ~ BQ-12 containing peppermint and Herba Schizonepetae extract, wherein, each parameter is in table 5.
Table 3
Table 4
Table 5
Embodiment 3 the present invention contains the preparation of the composition pesticide of peppermint and Herba Schizonepetae extract
Take menthol and D-menthone and auxiliary agent according to proportioning in table 6, mixing, stir, stirring and evenly mixing, obtains the composition pesticide BQ-13 ~ BQ-18 containing peppermint and Herba Schizonepetae extract.
Table 6
Wherein, menthol and D-menthone are all purchased from Ze Lang bio tech ltd, Nanjing.
Test example 1 the present invention contains the composition pesticide apple canker efficiency test of peppermint and Herba Schizonepetae extract
1 test material
1.1 for studying thing and object: apple canker
1.2 medicament
Test medicine: BQ-01 ~ BQ-18;
Peppermint single dose (D1); Menthol single dose (D2); Schizonepeta single dose (D3); D-menthone single dose (D4);
Clear water contrast (CK).
Wherein, the preparation method of peppermint single dose is: the d1-03 getting 60g, and add 10g castor oil ethylene oxide condensate, 30g ethanol, stirring and evenly mixing, to obtain final product.
Schizonepeta single dose preparation method is: the d2-03 getting 60g, and add 10g castor oil ethylene oxide condensate, 30g ethanol, stirring and evenly mixing, to obtain final product.
The preparation method of menthol single dose is: the menthol getting 60g, and add 10g castor oil ethylene oxide condensate, 30g ethanol, stirring and evenly mixing, to obtain final product.
D-menthone single dose preparation method is: the D-menthone getting 60g, and add 10g castor oil ethylene oxide condensate, 30g ethanol, stirring and evenly mixing, to obtain final product.
2, experimental scheme and method
Small plot experiment is designed with reference to standard GB/T/T17980.117-2004 " field control effectiveness test criterion (two) bactericidal agent for preventing and treating apple canker ".
This test 18 medicaments, 4 single dose contrasts, 1 blank, repeats for three times, Gong69Ge community.Minizone and surrounding all leave isolation strip, plot area 12m
2, community respectively processes randomized arrangement.
By medicament BQ-01 ~ BQ-12, D1, D3 thin up to the activity component concentration of crude drug weighing scale for 50ppm; Be 50ppm by BQ-13 ~ BQ-18, D2, D4 thin up to activity component concentration; And spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Test adopts following stage division:
0 grade: anosis;
1 grade: hypopathia is set, and tree crown is substantially complete, then new sick piece of 1-2 block, without the serious disease scar that more than 20cm grows;
2 grades: middle disease tree, tree crown is substantially complete or once due to illness removed bough, newly sick piece more than 5 pieces, and mobile jib and major branch base portion have more more than 2 pieces more than 20cm old complaint scars;
3 grades: grave illness is set, and tree crown is imperfect, once due to illness removes multiple bough or major branch, newly sick piece more than 8 pieces, and mobile jib and major branch base portion have the above old complaint scar of more 3 piece of-5 block length 20cm;
According to survey data, calculate disease index and the control efficiency of each process.
Disease index is pressed formula (1) and is calculated, and result of calculation retains 2 significant digits:
Control efficiency is pressed formula (3) and is calculated, and result of calculation retains 2 significant digits:
3, result of the test
According to newly-increased sick block growth rate after above-mentioned stage division and the dispenser of data processing formula statistics, calculate control efficiency, the results are shown in Table 7.
Table 7
| Reagent agent | Newly-increased sick block growth rate after medicine | Control efficiency (%) |
| BQ-01 | 4.75 | 80.17 |
| BQ-02 | 4.91 | 79.50 |
| BQ-03 | 4.25 | 82.25 |
| BQ-04 | 3.74 | 84.38 |
| BQ-05 | 3.26 | 86.39 |
| BQ-06 | 3.99 | 83.34 |
| BQ-07 | 7.24 | 69.77 |
| BQ-08 | 7.61 | 68.23 |
| BQ-09 | 8.08 | 66.26 |
| BQ-10 | 8.35 | 65.14 |
| BQ-11 | 7.27 | 69.65 |
| BQ-12 | 8.16 | 65.93 |
| BQ-13 | 10.66 | 55.49 |
| BQ-14 | 11.54 | 51.82 |
| BQ-15 | 10.21 | 57.37 |
| BQ-16 | 11.46 | 52.15 |
| BQ-17 | 10.52 | 56.08 |
| BQ-18 | 10.69 | 55.37 |
| D1 | 15.68 | 34.53 |
| D2 | 15.52 | 35.20 |
| D3 | 16.14 | 32.61 |
| D4 | 15.98 | 33.28 |
| CK | 23.95 | -- |
As shown in Table 7, the present invention has good preventive effect containing the composition pesticide of peppermint and Herba Schizonepetae extract to apple canker.
The preventive effect of the composition pesticide (BQ-01 ~ BQ-06) adopting common extracting method to make is apparently higher than the composition pesticide (BQ-07 ~ BQ-012) be mixed to get after extracting separately, visible common extraction contributes to improving dissolution rate, improves germicidal efficiency; And the control efficiency of schizonepeta single dose D3 that the peppermint single dose D1 be prepared from than single mint extract and Herba Schizonepetae extract are prepared from is good, visible, has synergistic function between product compositions of the present invention.
Simultaneously, adopt the preventive effect of menthol and the composite composition pesticide (BQ-13 ~ BQ-18) obtained of D-menthone also apparently higher than menthol single dose D2 and D-menthone single dose D4, visible, menthol and D-menthone is composite has synergistic function.In addition, have no during dispenser and have poisoning to show to plant.
Claims (10)
1. contain the composition pesticide of peppermint and Herba Schizonepetae extract, it is characterized in that, its active component is extracted by the crude drug of following weight portion and forms: peppermint 10 ~ 30 parts, schizonepeta 1 part; Preferably, its active component is extracted by the crude drug of following weight portion and forms: peppermint 15 ~ 25 parts, schizonepeta 1 part; Be more preferably: peppermint 22 parts, schizonepeta 1 part.
2. the composition pesticide of peppermint according to claim 1 and Herba Schizonepetae extract, is characterized in that: its active component jointly to be extracted through organic solution or water by peppermint and schizonepeta and forms; Or the Herba Schizonepetae extract that the mint extract to be obtained to through organic solution or water extraction by peppermint, schizonepeta obtain through organic solution or water extraction mixes; Described organic solvent is acetone, ethyl acetate, ethanol, methyl alcohol or chloroform; Further, described organic solvent is preferably ethanol or methyl alcohol.
3. the composition pesticide containing peppermint and Herba Schizonepetae extract according to claim 1 and 2, is characterized in that: the described composition pesticide containing peppermint and Herba Schizonepetae extract is microemulsion, soluble concentrate or missible oil.
4. the composition pesticide containing peppermint and Herba Schizonepetae extract according to claim 3, it is characterized in that, also comprise auxiliary agent containing in the composition pesticide of peppermint and Herba Schizonepetae extract, described auxiliary agent is at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
5. the composition pesticide containing peppermint and Herba Schizonepetae extract according to claim 4, it is characterized in that, the composition pesticide containing peppermint and Herba Schizonepetae extract is soluble concentrate,
Described soluble concentrate is made up of the component of following weight portion: peppermint and Herba Schizonepetae extract, dibenzyl xenyl APEO 6 parts, ethanol 44 parts; Wherein, peppermint and Herba Schizonepetae extract are that the crude drug of 50 weight portions jointly extracts and forms; Peppermint is prepared from by the following method with Herba Schizonepetae extract: crude drug is through extracting and being concentrated into identical with crude drug weight;
Or: described soluble concentrate is made up of the component of following weight portion: peppermint and Herba Schizonepetae extract, 10g castor oil ethylene oxide condensate, 30g ethanol; Wherein, peppermint and Herba Schizonepetae extract are that the crude drug of 60 weight portions jointly extracts and forms; Peppermint is prepared from by the following method with Herba Schizonepetae extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
6. contain the composition pesticide of peppermint and Herba Schizonepetae extract, it is characterized in that: its active component is made up of menthol and D-menthone, wherein, the mass ratio of menthol and D-menthone is 1:0.1 ~ 1, is preferably 1:0.4.
7. the preparation method containing the composition pesticide of peppermint and Herba Schizonepetae extract described in any one of Claims 1 to 5, is characterized in that being made up of following steps:
A, extraction: take mint particles and schizonepeta particle, mixing, adds solvent by solid-liquid ratio 1:8 ~ 12g/ml, and in 55 ~ 100 DEG C of refluxing extraction, filter, obtain extract, wherein, described solvent is organic solvent or water;
B, preparation: extract is made microemulsion, soluble concentrate or missible oil.
8. the preparation method containing the composition pesticide of peppermint and Herba Schizonepetae extract according to claim 7, it is characterized in that: in described a step, mint particles and schizonepeta particle cross 60 ~ 100 mesh sieves, preferred mistake 80 mesh sieve; Solid-liquid ratio is 1:10 ~ 12g/ml; Organic solvent is methyl alcohol, ethanol, ethyl acetate, acetone, chloroform, is preferably 95%v/v ethanol or methyl alcohol; Reflux temperature is 65 ~ 85 DEG C; Reflow's cycle is twice, and merging filtrate, obtains extract.
9. the composition pesticide containing peppermint and Herba Schizonepetae extract described in any one of Claims 1 to 5 is preventing and treating the application in crops apples rot; Further, during application, be diluted with water to the activity component concentration of crude drug weighing scale for 10 ~ 100ppm, be more preferably diluted with water to the activity component concentration of crude drug weighing scale as 20 ~ 50ppm.
10. the described composition pesticide containing peppermint and Herba Schizonepetae extract according to claim 6 is preventing and treating the application in crops apples rot; Further, during application, to be diluted with water to active component be concentration is 10 ~ 100ppm, and to be more preferably diluted with water to active component be concentration is 20 ~ 50ppm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510882977.5A CN105340986A (en) | 2015-12-04 | 2015-12-04 | Pesticide composition containing mint and schizonepeta extract as well as preparation method and application of pesticide composition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510882977.5A CN105340986A (en) | 2015-12-04 | 2015-12-04 | Pesticide composition containing mint and schizonepeta extract as well as preparation method and application of pesticide composition |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105340986A true CN105340986A (en) | 2016-02-24 |
Family
ID=55317272
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510882977.5A Withdrawn CN105340986A (en) | 2015-12-04 | 2015-12-04 | Pesticide composition containing mint and schizonepeta extract as well as preparation method and application of pesticide composition |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105340986A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114903040A (en) * | 2022-06-24 | 2022-08-16 | 安徽农业大学 | Application of menthone in prevention and treatment of tea tree diseases |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1100594A (en) * | 1993-09-23 | 1995-03-29 | 李百川 | Pesticide for fruit trees and method for prepn. of same |
| CN1382380A (en) * | 2002-01-24 | 2002-12-04 | 岳秀梅 | Composite chemical for preventing and treating bark rot of tree |
| CN1631172A (en) * | 2004-11-18 | 2005-06-29 | 丁小武 | Prophylaxis insect repellant made from pure plants and its making method |
-
2015
- 2015-12-04 CN CN201510882977.5A patent/CN105340986A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1100594A (en) * | 1993-09-23 | 1995-03-29 | 李百川 | Pesticide for fruit trees and method for prepn. of same |
| CN1382380A (en) * | 2002-01-24 | 2002-12-04 | 岳秀梅 | Composite chemical for preventing and treating bark rot of tree |
| CN1631172A (en) * | 2004-11-18 | 2005-06-29 | 丁小武 | Prophylaxis insect repellant made from pure plants and its making method |
Non-Patent Citations (1)
| Title |
|---|
| 武策等: ""库尔勒香梨树腐烂病生物防治方法的探究"", 《中学生物学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114903040A (en) * | 2022-06-24 | 2022-08-16 | 安徽农业大学 | Application of menthone in prevention and treatment of tea tree diseases |
| CN114903040B (en) * | 2022-06-24 | 2023-10-17 | 安徽农业大学 | Application of Menthone in Tea Tree Disease Control |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104186562B (en) | A kind of biogenic synergistic complex herbicide containing n-nonanoic acid and preparation method thereof | |
| CN103704281B (en) | Containing the composition pesticide and its preparation method and application of kuh-seng and tobacco extract | |
| CN103651632B (en) | Containing the composition pesticide and its preparation method and application of Fructus meliae toosendan and black false hellebore extract | |
| CN103651605B (en) | Containing composition pesticide and the preparation method and application of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract | |
| CN103651608B (en) | Containing composition pesticide and the preparation method and application of kitchen garden and aconite extract | |
| CN101176451A (en) | Damanling suspension agent | |
| CN103651602B (en) | Pesticide composition containing thymus mongolicus and common origanum extracts, and preparation method and application thereof | |
| CN103704278B (en) | Containing composition pesticide and the preparation method and application of Fructus meliae toosendan and triperygium wilfordii extractive | |
| CN105284913A (en) | Pesticide composition containing sophora alopecuroide and vetchleaf sophora root extract as well as preparation method and application thereof | |
| CN103734204B (en) | Pesticide composite containing schizonepeta and tobacco extract, preparation method and application | |
| CN105284914A (en) | Pesticide composition containing cynanchum komarovii and sophora alopecuroide extract as well as preparation method and application thereof | |
| CN105340986A (en) | Pesticide composition containing mint and schizonepeta extract as well as preparation method and application of pesticide composition | |
| CN103689002B (en) | Containing composition pesticide and the preparation method and application of lantana and Azolla imbricata extract | |
| CN103651597B (en) | Containing the agricultural chemicals and its production and use of acalypha copperleaf and Derris ellipta extract | |
| CN103651592B (en) | Containing composition pesticide and the preparation method and application of Dalmatian chrysanthemum and extract from Lantana camara | |
| CN105284967A (en) | Pesticide composition containing extracts of cymbopogon distans and raw gingers and preparation method and application thereof | |
| CN105475379A (en) | Pesticide composition containing fructus aurantii immaturus extract and cinnamon extract, and preparation method and application thereof | |
| CN105284915A (en) | Pesticide composition containing extracts of folium isatidis and thyme and preparation method and application thereof | |
| CN103651596B (en) | Containing composition pesticide and the preparation method and application of kuh-seng and Artabotrys odoratissimus extract | |
| CN105309516A (en) | Pesticide composition containing garlic and tea leaf extracts as well as preparation method and application thereof | |
| CN103704285B (en) | Pesticide composition containing extracts of veratrum nigrum and mamee-apple and preparation method and application of pesticide combination | |
| KR20110029297A (en) | A natural pesticidal composition and pesticidal method using the same | |
| CN103651595B (en) | Pesticide composition containing lightyellow sophora root and Caesalpinia crista extracts, and preparation method and application thereof | |
| CN104381330A (en) | Pesticide composition containing cortex dictamni and rubia cordifolia extract as well as preparation method and application of pesticide composition | |
| CN104472572A (en) | Pesticide composition containing fructus kochiae extract and radix peucedani extract, preparation method and application of pesticide composition |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information |
Address after: 611630 No. five, No. 35 Industrial Road, Heshan Town, Pujiang County, Sichuan, Chengdu Applicant after: Chengdu new Chaoyang Crop Science Co., Ltd Address before: 611630 No. five, No. 35 Industrial Road, Heshan Town, Pujiang County, Sichuan, Chengdu Applicant before: CHENGDU NEWSUN CROPSCIENCE Co.,Ltd. |
|
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20160224 |
|
| WW01 | Invention patent application withdrawn after publication |