CN103651595B - Pesticide composition containing lightyellow sophora root and Caesalpinia crista extracts, and preparation method and application thereof - Google Patents
Pesticide composition containing lightyellow sophora root and Caesalpinia crista extracts, and preparation method and application thereof Download PDFInfo
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- CN103651595B CN103651595B CN201310743607.4A CN201310743607A CN103651595B CN 103651595 B CN103651595 B CN 103651595B CN 201310743607 A CN201310743607 A CN 201310743607A CN 103651595 B CN103651595 B CN 103651595B
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Abstract
The invention relates to a pesticide composition containing lightyellow sophora root and Caesalpinia crista extracts, and a preparation method and application thereof, and belongs to the field of pesticides. In order to solve technical problems, the invention provides the pesticide composition containing the lightyellow sophora root and Caesalpinia crista extracts, and the preparation method and application thereof. According to the pesticide composition containing the lightyellow sophora root and Caesalpinia crista extracts, active ingredients are extracted from 1 weight part of lightyellow sophora root and 0.2 to 4 weight parts of Caesalpinia crista; the active ingredients can be prepared by mixing the lightyellow sophora root and the Caesalpinia crista and extracting together, or prepared by separately extracting the lightyellow sophora root to obtain the lightyellow sophora root extract, separately extracting the Caesalpinia crista to obtain the Caesalpinia crista extract, and mixing the two extracts. The pesticide composition containing the lightyellow sophora root and Caesalpinia crista extracts has an obvious effect of controlling Homoptera, Acarina, Thysanoptera and Lepidoptera pests, and has positive significance for agricultural production.
Description
Technical field
The present invention relates to composition pesticide and preparation method and application containing kuh-seng and caesalpinia nuga extract, belong to pesticide field.
Background technology
In recent years, in agriculture and forestry field, due to single drug and Scientific Usage of Drugs not, plant pest has produced pesticide resistance in various degree, and agriculture and forestry are produced still with chemical pesticide as mainly preventing and treating kind, have caused the destruction increasingly of environmental pollution and ecotope.Along with the raising of people's living standard and the enhancing of health perception, the development of biopesticide and exploitation just progressively receive agricultural chemicals R&D worker's concern, become the focus of agriculture and forestry sustainable development, simultaneously, also be to improve environment, the effective way of minimizing or delaying drug resistance and raising drug effect.Wherein, botanical pesticide has become one of focus of biopesticide research.
Kuh-seng, the plant that pulse family kuh-seng belongs to.In its root, containing matrine, oxymatrine, N-, be oxidized the multiple alkaloids such as sophocarpine, Sophoridine, Iosmatrine, counterfeit indigo leaf alkali; Also containing multiple flavonoidss such as the new alcohol of kuh-seng, kuh-seng chalcone, kuh-seng alcohol, formoononetin, kurarinone, maackiain, kushenin, Xanthohumol, different fulvic acids; In addition, also contain triterpenoid saponin and quinones.Find after deliberation, kuh-seng has the effect of anti-inflammation, and Propiobacterium, staphylococcus aureus, Escherichia coli are all had to inhibitory action.In recent years, matrine is extensive use in agricultural, and has good control efficiency, is a kind of low toxicity, low-residual, environmental protection type agricultural chemical.
Caesalpinia nuga, has another name called false Lao Hu le, the plant that pulse family mysorethorn belongs to.Originate in Yunnan, Guizhou, Sichuan, Hubei, Hunan, Guangxi, Guangdong, Fujian and Taiwan.Contained chemical composition mainly be take diterpene and homoisoflavone as main, and its root, stem and fruit can be used for medicinal purpose, and has effect that delivering loose cold, activating blood to promote menstruation, removing toxic substances desinsection.
In test, the present inventor finds that kuh-seng and the common extract of caesalpinia nuga and shrubby sophora extract and caesalpinia nuga extract mix and have good synergistic function, can be prepared into insecticide, aphid, scale insect, tetranychid, aleyrodid, thrips, cabbage caterpillar, diamond-back moth, prodenia litura etc. are had to good preventive effect.
Summary of the invention
The technical problem to be solved in the present invention is to provide composition pesticide and the preparation method and application containing kuh-seng and caesalpinia nuga extract.
The present invention is containing the composition pesticide of kuh-seng and caesalpinia nuga extract, and its active component is formed by the crude drug extraction of following weight portion: 1 part of kuh-seng, 0.2～4 part of caesalpinia nuga.Wherein, active component is preferably formed by the crude drug extraction of following weight portion: 1 part of kuh-seng, 0.5～2 part of caesalpinia nuga; The crude drug extraction being preferably again by following weight portion forms: 1 part of kuh-seng, 0.5～1.5 part of caesalpinia nuga; The more preferably crude drug extraction by following weight portion forms: 1 part of kuh-seng, 1 part of caesalpinia nuga.
Wherein, kuh-seng and caesalpinia nuga that the present invention adopts are Chinese herbal medicine, and in order to improve the present invention containing the activity of the composition pesticide of kuh-seng and caesalpinia nuga extract, kuh-seng is dry radix sophorae preferably, caesalpinia nuga is dry caesalpinia nuga acrial part preferably, as stem, leaf etc.
The present invention is containing the composition pesticide of kuh-seng and caesalpinia nuga extract, and after its active component can be mixed by crude drug kuh-seng and caesalpinia nuga, common extraction forms; Also can first kuh-seng be extracted and obtain shrubby sophora extract separately, caesalpinia nuga extracts and obtains caesalpinia nuga extract separately, then two kinds of extracts are mixed.In order to improve the insecticidal properties of composition pesticide of the present invention, after its active component is preferably and is mixed by kuh-seng and caesalpinia nuga, common extraction forms.
Further, in order to improve the stability containing the composition pesticide of kuh-seng and caesalpinia nuga extract, in the composition pesticide of the present invention containing kuh-seng and caesalpinia nuga extract, also comprise auxiliary agent, described auxiliary agent can adopt the usual auxiliaries in agricultural chemicals, as at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
Wherein, emulsifier can adopt at least one in non-ionic surface active agent and anion surfactant, described non-ionic surface active agent can be dibenzyl xenyl APEO, styryl phenyl APEO, styryl phenyl APEO, phenethyl phenyl polyoxyethylene one polyethenoxy ether, OPEO, alkylphenol polyoxyethylene sulfosuccinate, aliphatic acid polyethenoxy ether, fatty alcohol-polyoxyethylene ether, aliphatic amine polyoxyethylene ether, castor oil ethylene oxide adduct and derivative thereof, at least one in polyol fatty acid ester and ethylene oxide adduct etc. thereof, described anion surfactant is at least one in calcium dodecyl benzene sulfonate, alkylphenol polyoxyethylene phosphate, phenethyl phenol polyethenoxy ether phosphate etc., as emulsifier 500,601p, 602p etc., in addition, can also use commercially available emulsifier production.
Wherein, solvent can adopt at least one in water, ethanol, isopropyl alcohol, n-butanol, ethyl acetate, butyl acetate, methyl-sulfoxide, turpentine oil, solvent naphtha, 1-METHYLPYRROLIDONE, dimethyl formamide etc.
Wherein, antifreeze can be glycerine, propane diols, ethylene glycol, sodium chloride, calcium chloride, sorbierite etc.
Wherein, defoamer is silicone defoaming agent etc.
Wherein, dispersant is at least one in lignosulfonates, PAA and propionamide copolymer, alkylnaphthalene sulfonate, polycarboxylic acid salt's condensation product, methyl sodiosul foaliphatate etc.
Wherein, wetting agent can be alkyl sulfate, alkylbenzenesulfonate, alkylnaphthalene sulfonate, aliphatic alcohol polyethenoxy base ether, alkylphenol-polyethenoxy base ether, sorbitan fatty acid ester etc.
Wherein, stabilizing agent can be 2-expoxy propane, butyl glycidyl ether, isopropyl alcohol, n-butanol, polyethylene glycol etc.
The present invention can be prepared into by the method for this area routine containing the composition pesticide of kuh-seng and caesalpinia nuga extract any one formulation that is applicable to agricultural use, for the ease of storing and transportation, can make microemulsion, aqua or soluble concentrate.
Further, be preferably the aqua that the component by following weight portion forms: kuh-seng and caesalpinia nuga extract, 10 parts of ethanol, 1601,5 parts of ethylene glycol of 10 parts of emulsifier, 45 parts of water, wherein, kuh-seng and caesalpinia nuga extract are that the crude drug of 30 parts extracts and forms.
For the ease of storing and transportation, can extract is concentrated, obtain concentrate, and then be prepared into various formulations, cycles of concentration can be adjusted voluntarily according to needs of production, as preferred version, kuh-seng is prepared from by the following method with caesalpinia nuga extract: crude drug is identical with crude drug weight through extracting and being concentrated into.
Second technical problem that the present invention will solve is to provide preparation containing the method for the composition pesticide of kuh-seng and caesalpinia nuga extract.
The present invention is comprised of following steps containing the preparation method of the composition pesticide of kuh-seng and caesalpinia nuga extract:
A, extraction: take kuh-seng particle and caesalpinia nuga particle, mix, by solid-liquid ratio, 1:3～12g/ml adds solvent, in 60～95 ℃ of refluxing extraction, filter, and obtains extract, and wherein, described solvent is organic solvent or water;
B, preparation: extract is made to microemulsion, soluble concentrate or aqua.
Wherein, kuh-seng particle and caesalpinia nuga particle can adopt commercially available particle, also can adopt conventional method pulverizing radix sophorae and caesalpinia nuga cauline leaf to obtain.In order to improve the effect of extraction, simultaneously cost-saving, kuh-seng particle and caesalpinia nuga particle are crossed 60～100 mesh sieves, preferred mistake 80 mesh sieves.
Further, for cost-saving, simultaneously also in order to improve extraction efficiency, the solid-liquid ratio in a step is preferably 1:4～10g/ml, more preferably 1:6g/ml.The kuh-seng and the caesalpinia nuga particle that are lg can add 3～12ml solvent extraction, preferably add 4～10ml, more preferably add 6ml.
Conventional organic solvent all can be used for the present invention, for cost-saving, improves dissolution rate, guarantees extraction efficiency simultaneously, and organic solvent is preferably methyl alcohol, ethanol, ethyl acetate, acetone, chloroform, the ethanol that more preferably volume fraction is 95%.
In order to shorten preparation time, accelerate extraction rate, can when adding hot reflux, stir, mixing speed is preferably 80～100 revs/min.
Further, reflux temperature is preferably 75～85 ℃, more preferably 82 ℃; Reflow's cycle is twice, and each return time is 2 hours, and merging filtrate, obtains extract.
The present invention also provides the application in control crop pest of composition pesticide that the present invention contains kuh-seng and caesalpinia nuga extract.The present invention is containing the composition pesticide of kuh-seng and caesalpinia nuga extract, to homoptera pest as aphid, scale insect, trialeurodes vaporariorum etc.; Acarina pest is as panonychus citri, Tetranychus cinnabarinus etc.; Thrips insect is as thrips; Lepidoptera pest has good preventive effect as cabbage caterpillar, diamond-back moth, prodenia litura, beet snout moth's larva, tea tree tea geometrid etc.
In actual agricultural production, in order to improve composition pesticide containing kuh-seng and the caesalpinia nuga extract control efficiency to insect pest, the described composition pesticide containing kuh-seng and caesalpinia nuga extract is preferably applied to crop pest initial stage of origination; The present invention can use by common method containing the composition pesticide of kuh-seng and caesalpinia nuga extract, sprays on crop blade face.As preferred version, while using, the present invention is diluted with water to containing the composition pesticide of kuh-seng and caesalpinia nuga extract to take the activity component concentration of crude drug weighing scale be 10～100ppm, it is the composition pesticide making after 1g crude drug extracts, when using, be diluted with water to 10kg, its activity component concentration equals 100ppm.Further preferably being diluted to activity component concentration is that 20～50ppm sprays in plant blade face, and to occur water membrane on blade face, not lower of liquid is advisable; After being preferable over 3～5 days, carry out secondary dispenser, the preferred continuous administration of a season crop 2～3 times.
The present invention is mixed by shrubby sophora extract and caesalpinia nuga extract containing the composition pesticide of kuh-seng and caesalpinia nuga extract, or formed by kuh-seng and common extraction of caesalpinia nuga, its preventive effect is far away higher than the extract of single component, particularly kuh-seng and caesalpinia nuga are mixed to rear common extraction, owing to having solubilising, hydrotropy effect between kuh-seng and caesalpinia nuga, the composition pesticide insecticidal effect of making after common extraction is more remarkable.
Compared with prior art, the present invention has the following advantages containing the composition pesticide of kuh-seng and caesalpinia nuga extract:
1, the present invention has synergistic function containing the composition pesticide of kuh-seng and caesalpinia nuga extract, obvious to the control efficiency of the insects such as Homoptera, Acarina, thrips, Lepidoptera.
2, kuh-seng of the present invention and caesalpinia nuga are common extracts or extracts respectively rear mixing, and the composition pesticide of making can improve crop immune system ability in quick-acting tagging, and can significantly improve its vigor.
3, the present invention be take Chinese herbal medicine as raw material, thereby does not have Pesticide Residue, and insect is difficult to produce resistance; Compare with independent use chemical source agricultural chemicals, environmentally friendly.
When 4, the present invention uses containing the composition pesticide of kuh-seng and caesalpinia nuga extract, valid density is low, with low cost.
Embodiment
The present invention is containing the composition pesticide of kuh-seng and caesalpinia nuga extract, and its active component is formed by the crude drug extraction of following weight portion: 1 part of kuh-seng, 0.2～4 part of caesalpinia nuga.Wherein, active component is preferably formed by the crude drug extraction of following weight portion: 1 part of kuh-seng, 0.5～2 part of caesalpinia nuga; The crude drug extraction being preferably again by following weight portion forms: 1 part of kuh-seng, 0.5～1.5 part of caesalpinia nuga; The more preferably crude drug extraction by following weight portion forms: 1 part of kuh-seng, 1 part of caesalpinia nuga.
Wherein, kuh-seng and caesalpinia nuga that the present invention adopts are Chinese herbal medicine, and in order to improve the present invention containing the activity of the composition pesticide of kuh-seng and caesalpinia nuga extract, kuh-seng is dry radix sophorae preferably, caesalpinia nuga is dry caesalpinia nuga acrial part preferably, as stem, leaf etc.
The present invention is containing the composition pesticide of kuh-seng and caesalpinia nuga extract, and after its active component can be mixed by crude drug kuh-seng and caesalpinia nuga, common extraction forms; Also can first kuh-seng be extracted and obtain shrubby sophora extract separately, caesalpinia nuga extracts and obtains caesalpinia nuga extract separately, then two kinds of extracts are mixed.In order to improve the insecticidal properties of composition pesticide of the present invention, after its active component is preferably and is mixed by kuh-seng and caesalpinia nuga, common extraction forms.
Further, in order to improve the stability containing the composition pesticide of kuh-seng and caesalpinia nuga extract, in the composition pesticide of the present invention containing kuh-seng and caesalpinia nuga extract, also comprise auxiliary agent, described auxiliary agent can adopt the usual auxiliaries in agricultural chemicals, as at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
Wherein, emulsifier can adopt at least one in non-ionic surface active agent and anion surfactant, described non-ionic surface active agent can be dibenzyl xenyl APEO, styryl phenyl APEO, styryl phenyl APEO, phenethyl phenyl polyoxyethylene one polyethenoxy ether, OPEO, alkylphenol polyoxyethylene sulfosuccinate, aliphatic acid polyethenoxy ether, fatty alcohol-polyoxyethylene ether, aliphatic amine polyoxyethylene ether, castor oil ethylene oxide adduct and derivative thereof, at least one in polyol fatty acid ester and ethylene oxide adduct etc. thereof, described anion surfactant is at least one in calcium dodecyl benzene sulfonate, alkylphenol polyoxyethylene phosphate, phenethyl phenol polyethenoxy ether phosphate etc., as emulsifier 500,601p, 602p etc., in addition, can also use commercially available emulsifier production.
Wherein, solvent can adopt at least one in water, ethanol, isopropyl alcohol, n-butanol, ethyl acetate, butyl acetate, methyl-sulfoxide, turpentine oil, solvent naphtha, 1-METHYLPYRROLIDONE, dimethyl formamide etc.
Wherein, antifreeze can be glycerine, propane diols, ethylene glycol, sodium chloride, calcium chloride, sorbierite etc.
Wherein, defoamer is silicone defoaming agent etc.
Wherein, dispersant is at least one in lignosulfonates, PAA and propionamide copolymer, alkylnaphthalene sulfonate, polycarboxylic acid salt's condensation product, methyl sodiosul foaliphatate etc.
Wherein, wetting agent can be alkyl sulfate, alkylbenzenesulfonate, alkylnaphthalene sulfonate, aliphatic alcohol polyethenoxy base ether, alkylphenol-polyethenoxy base ether, sorbitan fatty acid ester etc.
Wherein, stabilizing agent can be 2-expoxy propane, butyl glycidyl ether, isopropyl alcohol, n-butanol, polyethylene glycol etc.
The present invention can be prepared into by the method for this area routine containing the composition pesticide of kuh-seng and caesalpinia nuga extract any one formulation that is applicable to agricultural use, for the ease of storing and transportation, can make microemulsion, aqua or soluble concentrate.
Further, be preferably the aqua that the component by following weight portion forms: kuh-seng and caesalpinia nuga extract, 10 parts of ethanol, 1601,5 parts of ethylene glycol of 10 parts of emulsifier, 45 parts of water, wherein, kuh-seng and caesalpinia nuga extract are that the crude drug of 30 parts extracts and forms.
For the ease of storing and transportation, can extract is concentrated, obtain concentrate, and then be prepared into various formulations, cycles of concentration can be adjusted voluntarily according to needs of production, as preferred version, kuh-seng is prepared from by the following method with caesalpinia nuga extract: crude drug is identical with crude drug weight through extracting and being concentrated into.
The present invention is comprised of following steps containing the preparation method of the composition pesticide of kuh-seng and caesalpinia nuga extract:
A, extraction: take kuh-seng particle and caesalpinia nuga particle, mix, by solid-liquid ratio, 1:3～12g/ml adds solvent, in 60～95 ℃ of refluxing extraction, filter, and obtains extract, and wherein, described solvent is organic solvent or water;
B, preparation: extract is made to microemulsion, soluble concentrate or aqua.
Wherein, kuh-seng particle and caesalpinia nuga particle can adopt commercially available particle, also can adopt conventional method pulverizing radix sophorae and caesalpinia nuga cauline leaf to obtain.In order to improve the effect of extraction, simultaneously cost-saving, kuh-seng particle and caesalpinia nuga particle are crossed 60～100 mesh sieves, preferred mistake 80 mesh sieves.
Further, for cost-saving, simultaneously also in order to improve extraction efficiency, the solid-liquid ratio in a step is preferably 1:4～10g/ml, more preferably 1:6g/ml.The kuh-seng and the caesalpinia nuga particle that are lg can add 3～12ml solvent extraction, preferably add 4～10ml, more preferably add 6ml.
Conventional organic solvent all can be used for the present invention, for cost-saving, improves dissolution rate, guarantees extraction efficiency simultaneously, and organic solvent is preferably methyl alcohol, ethanol, ethyl acetate, acetone, chloroform, the ethanol that more preferably volume fraction is 95%.
In order to shorten preparation time, accelerate extraction rate, can when adding hot reflux, stir, mixing speed is preferably 80～100 revs/min.
Further, reflux temperature is preferably 75～85 ℃, more preferably 82 ℃; Reflow's cycle is twice, and each return time is 2 hours, and merging filtrate, obtains extract.
The present invention is containing the composition pesticide of kuh-seng and caesalpinia nuga extract, to homoptera pest as aphid, scale insect, trialeurodes vaporariorum etc.; Acarina pest is as panonychus citri, Tetranychus cinnabarinus etc.; Thrips insect is as thrips; Lepidoptera pest has good preventive effect as cabbage caterpillar, diamond-back moth, prodenia litura, beet snout moth's larva, tea tree tea geometrid etc.
In actual agricultural production, in order to improve composition pesticide containing kuh-seng and the caesalpinia nuga extract control efficiency to insect pest, the described composition pesticide containing kuh-seng and caesalpinia nuga extract is preferably applied to crop pest initial stage of origination; The present invention can use by common method containing the composition pesticide of kuh-seng and caesalpinia nuga extract, sprays on crop blade face.As preferred version, while using, the present invention is diluted with water to containing the composition pesticide of kuh-seng and caesalpinia nuga extract to take the activity component concentration of crude drug weighing scale be 10～100ppm, it is the composition pesticide making after 1g crude drug extracts, when using, be diluted with water to 10kg, its activity component concentration equals 100ppm.Further preferably being diluted to activity component concentration is that 20～50ppm sprays in plant blade face, and to occur water membrane on blade face, not lower of liquid is advisable; After being preferable over 3～5 days, carry out secondary dispenser, the preferred continuous administration of a season crop 2～3 times.
The present invention is mixed by shrubby sophora extract and caesalpinia nuga extract containing the composition pesticide of kuh-seng and caesalpinia nuga extract, or formed by kuh-seng and common extraction of caesalpinia nuga, its preventive effect is far away higher than the extract of single component, particularly kuh-seng and caesalpinia nuga are mixed to rear common extraction, owing to having solubilising, hydrotropy effect between kuh-seng and caesalpinia nuga, the composition pesticide insecticidal effect of making after common extraction is more remarkable.
Below in conjunction with embodiment, the specific embodiment of the present invention is further described, does not therefore limit the present invention among described scope of embodiments.
Embodiment 1 is containing the preparation of the composition pesticide of kuh-seng and caesalpinia nuga extract
A, extraction: take dry radix sophorae and caesalpinia nuga cauline leaf, mix, pulverize, cross 80 mesh sieves, add extraction solution, refluxing extraction twice, filters, and merging filtrate is concentrated, makes extract weight equal the weight of crude drug, obtains extract kh-01～kh-06; Wherein, each parameter is in Table 1;
B, preparation: auxiliary agent add table 2 in extract kh-01～kh-06 in, stir, must be containing the composition pesticide KH-01～KH-06 of kuh-seng and caesalpinia nuga extract.
The preparation technology parameter of table 1 kuh-seng and caesalpinia nuga extract
Wherein, kuh-seng rhizome and caesalpinia nuga cauline leaf are all purchased from Anguo City Zhong Tian prepared slices of Chinese crude drugs Co., Ltd.
Table 2 is containing the formula of the composition pesticide of kuh-seng and caesalpinia nuga extract
Embodiment 2 is containing the preparation of the composition pesticide of kuh-seng and caesalpinia nuga extract
A, extraction: take dry radix sophorae, pulverize, cross 80 mesh sieves, add extraction solution, refluxing extraction twice, filters, and merging filtrate is concentrated, makes extract weight equal the weight of crude drug, obtains extract d1-01～d1-03, and wherein, each parameter is in Table 3;
Take dry caesalpinia nuga cauline leaf, pulverize, cross 80 mesh sieves, add extraction solution, refluxing extraction twice, filters, and merging filtrate is concentrated, makes extract weight equal the weight of crude drug, obtains extract d2-01～d2-03, and wherein, each parameter is in Table 4;
B, preparation: take Radix Sophorae Flavescentis extractive liquid, caesalpinia nuga extract and auxiliary agent, stirring and evenly mixing, obtains the composition pesticide KH-07～KH-12 containing kuh-seng and caesalpinia nuga extract, and wherein, each parameter is in Table 5.
Table 3
Table 4
Table 5
Test example 1 the present invention is containing the efficiency test of the composition pesticide oranges and tangerines aphid of kuh-seng and caesalpinia nuga extract
For clearly putting into field efficacy situation containing the composition pesticide of kuh-seng and caesalpinia nuga extract, inventor carries out field control effectiveness test to this medicament.
1, test material
1.1 for studying thing and object: oranges and tangerines aphid
1.2 reagent agent
Product in embodiment: KH-01～KH-12;
Kuh-seng single dose (D1), caesalpinia nuga single dose (D2):
Chemical reagent contrast (CK-01): 10% Methomyl Imidacloprid, purchased from Hailir Pharmaceutical Group Co., Ltd.;
Clear water contrast (CK-02).
Wherein, the preparation method of kuh-seng single dose is: get the d1-03 of 30g, add 10g ethanol, and 10g emulsifier 1601,5g ethylene glycol, 45g water, stirring and evenly mixing, makes kuh-seng single dose.
The preparation method of caesalpinia nuga single dose is: gets the d2-03 of 30g, adds 10g ethanol, and 10g emulsifier 1601,5g ethylene glycol, 45g water, stirring and evenly mixing, makes caesalpinia nuga single dose.
2, experimental scheme and method
14 medicaments of this test, 1 contrast medicament, 1 blank, repeats Gong48Ge community for three times.Minizone and surrounding all leave isolation strip, community area 20m
2, randomized arrangement is respectively processed in community.
By reagent agent thin up, to activity component concentration, be 50ppm, spray application to plant blade face, to occur water membrane on blade face, not lower of liquid is advisable.
Before test, carry out Aphed population statistics alive the listing mark of fixed blade.Within 5 days after dispenser, investigate and record the aphid alive on each blade.Calculate insect population decline rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
3, result of the test
Within 5 days after dispenser, investigate and record the borer population alive on each blade, calculate insect population decline rate and preventive effect, it the results are shown in Table 6.
Table 6
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Insect population decline rate (%) | Preventive effect (%) |
| KH-01 | 254 | 57 | 77.56 | 81.55 |
| KH-02 | 235 | 36 | 84.68 | 87.41 |
| KH-03 | 267 | 34 | 87.27 | 89.53 |
| KH-04 | 289 | 42 | 85.47 | 88.05 |
| KH-05 | 257 | 45 | 82.49 | 85.61 |
| KH-06 | 217 | 44 | 79.72 | 83.33 |
| KH-07 | 246 | 82 | 66.67 | 72.60 |
| KH-08 | 235 | 61 | 74.04 | 78.66 |
| KH-09 | 241 | 57 | 76.35 | 80.56 |
| KH-10 | 269 | 69 | 74.35 | 78.91 |
| KH-11 | 273 | 77 | 71.79 | 76.81 |
| KH-12 | 286 | 88 | 69.23 | 74.71 |
| D1 | 270 | 177 | 34.44 | 46.11 |
| D2 | 263 | 195 | 25.86 | 39.05 |
| CK-01 | 255 | 27 | 89.41 | 91.30 |
| CK-02 | 268 | 326 | -21.64 | —— |
As can be seen from Table 6, product of the present invention has good preventive effect to aphid, and better, visible than the control efficiency of single shrubby sophora extract and caesalpinia nuga extract, between product compositions of the present invention, has synergistic function.And the preventive effect that adopts the product that common extracting method makes is apparently higher than blended product after independent extraction, visible common extraction contributes to improve dissolution rate, improves insecticide efficiency.
Test example 2 the present invention are containing the efficiency test of the composition pesticide eggplant two spotted spider mite of kuh-seng and caesalpinia nuga extract
Product K H-01～KH-12 of the present invention is used for preventing and treating eggplant two spotted spider mite.12 medicaments of this test, 2 single doses, 1 blank, repeats Gong45Ge community for three times.Minizone and surrounding all leave isolation strip, community area 10m
2, randomized arrangement is respectively processed in community.
By reagent agent thin up, to activity component concentration, be 50ppm, spray application to plant blade face, to occur water membrane on blade face, not lower of liquid is advisable.
Before test, carry out two spotted spider mite quantity statistics alive the listing mark of fixed blade.Within 5 days after dispenser, investigate and record the two spotted spider mite number alive on each blade.Calculate insect population decline rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group meter that repeats to average, calculates insect population decline rate and preventive effect, and it the results are shown in Table 7.
Table 7
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Insect population decline rate (%) | Preventive effect (%) |
| KH-01 | 344 | 105 | 69.48 | 74.41 |
| KH-02 | 356 | 90 | 74.72 | 78.80 |
| KH-03 | 342 | 79 | 76.90 | 80.63 |
| KH-04 | 367 | 96 | 73.84 | 78.07 |
| KH-05 | 346 | 97 | 71.97 | 76.49 |
| KH-06 | 389 | 115 | 70.44 | 75.21 |
| KH-07 | 375 | 151 | 59.73 | 66.24 |
| KH-08 | 316 | 110 | 65.19 | 70.81 |
| KH-09 | 327 | 112 | 65.75 | 71.28 |
| KH-10 | 338 | 120 | 64.50 | 70.23 |
| KH-11 | 385 | 143 | 62.86 | 68.86 |
| KH-12 | 364 | 140 | 61.54 | 67.75 |
| D1 | 385 | 275 | 28.57 | 40.11 |
| D2 | 311 | 237 | 23.79 | 36.10 |
| CK-02 | 379 | 452 | -19.26 | —— |
As can be seen from Table 7, product of the present invention has good preventive effect to two spotted spider mite, and better, visible than the control efficiency of single shrubby sophora extract and caesalpinia nuga extract, between product compositions of the present invention, has synergistic function.And the preventive effect that adopts the product that common extracting method makes is apparently higher than blended product after independent extraction, visible common extraction contributes to improve dissolution rate, improves insecticide efficiency.In addition, during dispenser, have no crop is had to poisoning performance.
Test example 3 the present invention are containing the efficiency test of the composition pesticide eggplant thrips of kuh-seng and caesalpinia nuga extract
Product K H-01～KH-12 of the present invention is used for preventing and treating eggplant thrips.12 medicaments of this test, 2 single doses, 1 blank, repeats Gong45Ge community for three times.Minizone and surrounding all leave isolation strip, community area 20m
2, randomized arrangement is respectively processed in community.
By reagent agent thin up, to activity component concentration, be 50ppm, spray application to plant blade face, to occur water membrane on blade face, not lower of liquid is advisable.
Before test, carry out thrips quantity statistics alive the listing mark of fixed blade.Within 5 days after dispenser, investigate and record the thrips number alive on each blade.Calculate insect population decline rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group meter that repeats to average, calculates insect population decline rate and preventive effect, and its result is as shown in table 8.
Table 8
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Insect population decline rate (%) | Preventive effect (%) |
| KH-01 | 422 | 111 | 73.70 | 77.60 |
| KH-02 | 437 | 97 | 77.80 | 81.10 |
| KH-03 | 465 | 96 | 79.35 | 82.42 |
| KH-04 | 477 | 102 | 78.62 | 81.79 |
| KH-05 | 431 | 100 | 76.80 | 80.24 |
| KH-06 | 486 | 115 | 76.34 | 79.85 |
| KH-07 | 438 | 175 | 60.05 | 65.98 |
| KH-08 | 469 | 165 | 64.82 | 70.04 |
| KH-09 | 470 | 157 | 66.60 | 71.55 |
| KH-10 | 436 | 150 | 65.60 | 70.70 |
| KH-11 | 424 | 155 | 63.44 | 68.87 |
| KH-12 | 421 | 165 | 60.81 | 66.63 |
| D1 | 468 | 364 | 22.22 | 33.77 |
| D2 | 457 | 383 | 16.19 | 28.63 |
| CK-02 | 413 | 485 | -17.43 | —— |
As can be seen from Table 8, product of the present invention has good preventive effect to thrips, and better, visible than the control efficiency of single shrubby sophora extract and caesalpinia nuga extract, between product compositions of the present invention, has synergistic function.And the preventive effect that adopts the product that common extracting method makes is apparently higher than blended product after independent extraction, visible common extraction contributes to improve dissolution rate, improves insecticide efficiency.In addition, during dispenser, have no crop is had to poisoning performance.
Test example 4 the present invention are containing the efficiency test of the composition pesticide a variety of Chinese cabbage diamond-back moth of kuh-seng and caesalpinia nuga extract
Product K H-01～KH-12 of the present invention is used for preventing and treating a variety of Chinese cabbage diamond-back moth.12 medicaments of this test, 2 single doses, 1 blank, repeats Gong45Ge community for three times.Minizone and surrounding all leave isolation strip, community area 10m
2, randomized arrangement is respectively processed in community.
By reagent agent thin up, to activity component concentration, be 50ppm, spray application to plant blade face, to occur water membrane on blade face, not lower of liquid is advisable.
Before test, carry out diamond-back moth quantity statistics alive the listing mark of fixed blade.Within 5 days after dispenser, investigate and record the diamond-back moth number alive on each blade.Calculate insect population decline rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group meter that repeats to average, calculates insect population decline rate and preventive effect, and its result is as shown in table 9.
Table 9
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Insect population decline rate (%) | Preventive effect (%) |
| KH-01 | 533 | 108 | 79.74 | 81.89 |
| KH-02 | 521 | 69 | 86.76 | 88.16 |
| KH-03 | 564 | 57 | 89.89 | 90.97 |
| KH-04 | 548 | 68 | 87.59 | 88.91 |
| KH-05 | 573 | 88 | 84.64 | 86.27 |
| KH-06 | 541 | 93 | 82.81 | 84.63 |
| KH-07 | 512 | 153 | 70.12 | 73.29 |
| KH-08 | 537 | 122 | 77.28 | 79.69 |
| KH-09 | 499 | 104 | 79.16 | 81.37 |
| KH-10 | 525 | 116 | 77.90 | 80.25 |
| KH-11 | 535 | 132 | 75.33 | 77.95 |
| KH-12 | 592 | 154 | 73.99 | 76.75 |
Continued 9
| D1 | 584 | 382 | 34.59 | 41.53 |
| D2 | 579 | 410 | 29.19 | 36.71 |
| CK-02 | 564 | 631 | -11.88 | —— |
As can be seen from Table 9, product of the present invention has good preventive effect to diamond-back moth, and better, visible than the control efficiency of single shrubby sophora extract and caesalpinia nuga extract, between product compositions of the present invention, has synergistic function.And the preventive effect that adopts the product that common extracting method makes is apparently higher than blended product after independent extraction, visible common extraction contributes to improve dissolution rate, improves insecticide efficiency.In addition, during dispenser, have no crop is had to poisoning performance.
Test example 5 the present invention are containing the efficiency test of the composition pesticide wild cabbage prodenia litura of kuh-seng and caesalpinia nuga extract
Product K H-01～KH-12 of the present invention is used for preventing and treating wild cabbage prodenia litura.12 medicaments of this test, 2 single doses, 1 blank, repeats Gong45Ge community for three times.Minizone and surrounding all leave isolation strip, community area 10m
2, randomized arrangement is respectively processed in community.
By reagent agent thin up, to activity component concentration, be 50ppm, spray application to plant blade face, to occur water membrane on blade face, not lower of liquid is advisable.
Before test, carry out prodenia litura quantity statistics alive the listing mark of fixed blade.Within 5 days after dispenser, investigate and record the prodenia litura number alive on each blade.Calculate insect population decline rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group meter that repeats to average, calculates insect population decline rate and preventive effect, and its result is as shown in table 10.
Table 10
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Insect population decline rate (%) | Preventive effect (%) |
| KH-01 | 329 | 82 | 75.08 | 78.63 |
| KH-02 | 345 | 66 | 80.87 | 83.60 |
| KH-03 | 366 | 63 | 82.79 | 85.24 |
| KH-04 | 352 | 66 | 81.25 | 83.92 |
| KH-05 | 316 | 66 | 79.11 | 82.09 |
Continued 10
| KH-06 | 347 | 74 | 78.67 | 81.71 |
| KH-07 | 388 | 142 | 63.40 | 68.62 |
| KH-08 | 337 | 102 | 69.73 | 74.05 |
| KH-09 | 358 | 100 | 72.07 | 76.05 |
| KH-10 | 341 | 103 | 69.79 | 74.10 |
| KH-11 | 357 | 117 | 67.23 | 71.90 |
| KH-12 | 355 | 121 | 65.92 | 70.77 |
| D1 | 373 | 278 | 25.47 | 36.09 |
| D2 | 361 | 292 | 19.11 | 30.64 |
| CK-02 | 325 | 379 | -16.62 | —— |
As can be seen from Table 10, product of the present invention has good preventive effect to prodenia litura, and better, visible than the control efficiency of single shrubby sophora extract and caesalpinia nuga extract, between product compositions of the present invention, has synergistic function.And the preventive effect that adopts the product that common extracting method makes is apparently higher than blended product after independent extraction, visible common extraction contributes to improve dissolution rate, improves insecticide efficiency.In addition, during dispenser, have no crop is had to poisoning performance.
Claims (25)
1. contain the composition pesticide of kuh-seng and caesalpinia nuga extract, it is characterized in that, its active component is formed by the crude drug extraction of following weight portion: 1 part of kuh-seng, 0.2～4 part of caesalpinia nuga, and active component is got and formed by organic solvent or water extraction, described organic solvent is methyl alcohol, ethanol, ethyl acetate, acetone or chloroform.
2. the composition pesticide containing kuh-seng and caesalpinia nuga extract according to claim 1, is characterized in that, its active component is formed by the crude drug extraction of following weight portion: 1 part of kuh-seng, 0.5～2 part of caesalpinia nuga.
3. the composition pesticide containing kuh-seng and caesalpinia nuga extract according to claim 2, is characterized in that, its active component is formed by the crude drug extraction of following weight portion: 1 part of kuh-seng, 0.5～1.5 part of caesalpinia nuga.
4. the composition pesticide containing kuh-seng and caesalpinia nuga extract according to claim 3, is characterized in that, its active component is formed by the crude drug extraction of following weight portion: 1 part of kuh-seng, 1 part of caesalpinia nuga.
5. according to the composition pesticide containing kuh-seng and caesalpinia nuga extract described in claim 1～4 any one, it is characterized in that: after its active component is mixed by kuh-seng and caesalpinia nuga, common extraction forms; Or by kuh-seng, extract the shrubby sophora extract, the caesalpinia nuga that obtain and extract the caesalpinia nuga extract obtaining and mix.
6. according to the composition pesticide containing kuh-seng and caesalpinia nuga extract described in claim 1～4 any one, it is characterized in that: the described composition pesticide containing kuh-seng and caesalpinia nuga extract is microemulsion, aqua or soluble concentrate.
7. the composition pesticide containing kuh-seng and caesalpinia nuga extract according to claim 5, is characterized in that: the described composition pesticide containing kuh-seng and caesalpinia nuga extract is microemulsion, aqua or soluble concentrate.
8. according to the composition pesticide containing kuh-seng and caesalpinia nuga extract described in claim 1～4 any one, it is characterized in that: in the described composition pesticide containing kuh-seng and caesalpinia nuga extract, also comprise auxiliary agent, described auxiliary agent is at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
9. the composition pesticide containing kuh-seng and caesalpinia nuga extract according to claim 5, it is characterized in that: in the described composition pesticide containing kuh-seng and caesalpinia nuga extract, also comprise auxiliary agent, described auxiliary agent is at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
10. the composition pesticide containing kuh-seng and caesalpinia nuga extract according to claim 6, it is characterized in that: in the described composition pesticide containing kuh-seng and caesalpinia nuga extract, also comprise auxiliary agent, described auxiliary agent is at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
11. composition pesticides containing kuh-seng and caesalpinia nuga extract according to claim 7, it is characterized in that: in the described composition pesticide containing kuh-seng and caesalpinia nuga extract, also comprise auxiliary agent, described auxiliary agent is at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
12. composition pesticides containing kuh-seng and caesalpinia nuga extract according to claim 8, it is characterized in that: described aqua is comprised of the component of following weight portion: kuh-seng and caesalpinia nuga extract, 10 parts of ethanol, 10 parts of emulsifier 1601,5 parts of ethylene glycol, 45 parts of water, wherein, kuh-seng and caesalpinia nuga extract are that the crude drug extraction of 30 parts forms.
13. composition pesticides containing kuh-seng and caesalpinia nuga extract according to claim 9, it is characterized in that: described aqua is comprised of the component of following weight portion: kuh-seng and caesalpinia nuga extract, 10 parts of ethanol, 10 parts of emulsifier 1601,5 parts of ethylene glycol, 45 parts of water, wherein, kuh-seng and caesalpinia nuga extract are that the crude drug extraction of 30 parts forms.
14. composition pesticides containing kuh-seng and caesalpinia nuga extract according to claim 10, it is characterized in that: described aqua is comprised of the component of following weight portion: kuh-seng and caesalpinia nuga extract, 10 parts of ethanol, 10 parts of emulsifier 1601,5 parts of ethylene glycol, 45 parts of water, wherein, kuh-seng and caesalpinia nuga extract are that the crude drug extraction of 30 parts forms.
15. composition pesticides containing kuh-seng and caesalpinia nuga extract according to claim 11, it is characterized in that: described aqua is comprised of the component of following weight portion: kuh-seng and caesalpinia nuga extract, 10 parts of ethanol, 10 parts of emulsifier 1601,5 parts of ethylene glycol, 45 parts of water, wherein, kuh-seng and caesalpinia nuga extract are that the crude drug extraction of 30 parts forms.
16. composition pesticides containing kuh-seng and caesalpinia nuga extract according to claim 12, is characterized in that: kuh-seng is prepared from by the following method with caesalpinia nuga extract: crude drug is identical with crude drug weight through extracting and being concentrated into.
17. composition pesticides containing kuh-seng and caesalpinia nuga extract according to claim 13, is characterized in that: kuh-seng is prepared from by the following method with caesalpinia nuga extract: crude drug is identical with crude drug weight through extracting and being concentrated into.
18. composition pesticides containing kuh-seng and caesalpinia nuga extract according to claim 14, is characterized in that: kuh-seng is prepared from by the following method with caesalpinia nuga extract: crude drug is identical with crude drug weight through extracting and being concentrated into.
19. composition pesticides containing kuh-seng and caesalpinia nuga extract according to claim 15, is characterized in that: kuh-seng is prepared from by the following method with caesalpinia nuga extract: crude drug is identical with crude drug weight through extracting and being concentrated into.
The method of the composition pesticide containing kuh-seng and caesalpinia nuga extract described in 20. preparation claim 1～19 any one, is characterized in that being comprised of following steps:
A, extraction: take kuh-seng particle and caesalpinia nuga particle, mix, by solid-liquid ratio, 1:3～12g/ml adds solvent, in 60～95 ℃ of refluxing extraction, filter, and obtains extract, and wherein, described solvent is organic solvent or water;
B, preparation: extract is made to microemulsion, soluble concentrate or aqua.
21. preparations according to claim 20, containing the method for the composition pesticide of kuh-seng and caesalpinia nuga extract, is characterized in that: in described a step, kuh-seng particle and caesalpinia nuga particle are crossed 60～100 mesh sieves; Solid-liquid ratio is 1:4～10g/ml; Organic solvent is methyl alcohol, ethanol, ethyl acetate, acetone, chloroform; Reflux temperature is 75～85 ℃; Reflow's cycle is twice, and merging filtrate, obtains extract.
22. preparations according to claim 21, containing the method for the composition pesticide of kuh-seng and caesalpinia nuga extract, is characterized in that: in described a step, kuh-seng particle and caesalpinia nuga particle are crossed 80 mesh sieves; Solid-liquid ratio is 1:6g/ml; Organic solvent is that volume fraction is 95% ethanol; Reflux temperature is 82 ℃.
The application of the composition pesticide containing kuh-seng and caesalpinia nuga extract described in 23. claim 1～19 any one in control crop pest.
The application of 24. composition pesticides containing kuh-seng and caesalpinia nuga extract according to claim 23 in control crop pest, is characterized in that: described insect pest is Homoptera, Acarina, thrips, lepidoptera pest; During application, thin up is 10～100ppm to take the activity component concentration of crude drug weighing scale.
The application of 25. composition pesticides containing kuh-seng and caesalpinia nuga extract according to claim 24 in control crop pest, is characterized in that: thin up is 20～50ppm to take the activity component concentration of crude drug weighing scale.
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