CN103651605B - Containing composition pesticide and the preparation method and application of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract - Google Patents
Containing composition pesticide and the preparation method and application of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract Download PDFInfo
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Abstract
The present invention relates to the composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract and preparation method and application, belong to pesticide field.The technical problem to be solved in the present invention is to provide composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract and preparation method and application.The composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract of the present invention, its active component is extracted by the crude drug of following weight portion and forms: Fructus meliae toosendan 1 part, 0.5 ~ 8 part, lettuce; After active component can be mixed by crude drug Fructus meliae toosendan and lettuce, common extraction forms; Also first can be extracted separately by Fructus meliae toosendan and obtain Sichuan chinaberry extract, lettuce is extracted separately and obtains Caulis et Folium Lactucae sativae extract, then is mixed by two kinds of extracts.Composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract of the present invention can be good at killing lepidoptera pest, and especially Pyralidae, Sulfur butterfly, noctuid insect, as beet snout moth's larva, cabbage caterpillar, cotton bollworm etc., have positive effect for agricultural production.
Description
Technical field
The present invention relates to the composition pesticide and its preparation method and application containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, belong to pesticide field.
Background technology
Fructus meliae toosendan has another name called FRUCTUS TOOSENDAN, traditional Chinese medicine, is the ripening fruits of Meliaceae deciduous tree toosendanin.Mainly originate in the southern various places of China, superior with the product person in Sichuan, therefore have another name called Fructus meliae toosendan.Fructus meliae toosendan is cold in nature, bitter, is the one of qi-activating drug.Mainly enter Liver Channel, catharsis liver heat, promoting qi circulation and relieving pain, has the function except damp and hot, clearing away the liver-fire, pain relieving, desinsection, is that preparation is efficiently without the important source material of residual hazard free of contamination novel plant class agricultural chemicals.
Lettuce is the annual of composite family Lactuca or biennial vegetable, and it is a kind of very common edible vegetable.In lettuce, the content of carbohydrate is lower, and mineral salt, vitamin then content are abundanter, especially containing more nicotinic acid.Also containing a certain amount of trace element zinc, iron in lettuce, the potassium content in lettuce enriches, and is 27 times of sodium salt content.The nutrient component of lettuce is a lot, comprises protein, fat, carbohydrate, ash content, provitamin A, vitamin B1, vitamin B2, vitamin c, microelements of calcium, phosphorus, iron, potassium, magnesium, silicon etc. and food fiber etc.Every 100 grams of lettuces get over moisture content 96.4 grams, 0.6 gram, protein, 0.1 gram, fat, 1.9 grams, carbohydrate, raw fiber 0.4 gram, calcium 7 milligrams, 31 milligrams, phosphorus, iron 2 milligrams, carotin 0.02 milligram, thiamine 0.03 milligram, 0.02 milligram, vitamin b3, niacin 0.5 milligram.
Inventor finds through a large amount of experiments, Fructus meliae toosendan and the common extract of lettuce or by Sichuan chinaberry extract and Caulis et Folium Lactucae sativae extract mixing, active insecticidal components wherein has synergistic function, can be good at killing lepidoptera pest, especially Pyralidae, Sulfur butterfly, noctuid insect, as beet snout moth's larva, cabbage caterpillar, cotton bollworm etc., for agricultural production, there is positive effect.
Summary of the invention
The technical problem to be solved in the present invention is to provide the composition pesticide and its preparation method and application containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract.
The present invention contains the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, and its active component is extracted by the crude drug of following weight portion and forms: Fructus meliae toosendan 1 part, 0.5 ~ 8 part, lettuce.Wherein, active component is preferably extracted by the crude drug of following weight portion and forms: Fructus meliae toosendan 1 part, 2 ~ 5 parts, lettuce; Be preferably again and formed by the crude drug extraction of following weight portion: Fructus meliae toosendan 1 part, 3 ~ 4 parts, lettuce; Be more preferably and formed by the crude drug extraction of following weight portion: Fructus meliae toosendan 1 part, 4 parts, lettuce.
Wherein, the acrial parts such as the Fructus meliae toosendan that the present invention adopts and lettuce are Chinese herbal medicine, contain the activity of the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract in order to improve the present invention, the Fructus meliae toosendan that Fructus meliae toosendan is preferably dry, the lettuce haulm that lettuce is preferably dry.
The present invention contains the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, and after its active component can be mixed by crude drug Fructus meliae toosendan and lettuce, common extraction forms; Also first can be extracted separately by Fructus meliae toosendan and obtain Sichuan chinaberry extract, lettuce is extracted separately and obtains Caulis et Folium Lactucae sativae extract, then is mixed by two kinds of extracts.In order to improve the insecticidal properties of composition pesticide of the present invention, after its active component is preferably and is mixed by Fructus meliae toosendan and lettuce, common extraction forms.
Further, in order to improve the stability of the composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, of the present invention containing in the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract also comprises auxiliary agent, described auxiliary agent can adopt the usual auxiliaries in agricultural chemicals, as at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
Wherein, emulsifier can adopt at least one in non-ionic surface active agent and anion surfactant, described non-ionic surface active agent can be dibenzyl xenyl APEO, styryl phenyl APEO, styryl phenyl APEO, phenethyl phenyl polyoxyethylene one polyethenoxy ether, OPEO, alkylphenol polyoxyethylene sulfosuccinate, aliphatic acid polyethenoxy ether, fatty alcohol-polyoxyethylene ether, aliphatic amine polyoxyethylene ether, castor oil ethylene oxide adduct and derivative thereof, at least one in polyol fatty acid ester and ethylene oxide adduct etc. thereof, described anion surfactant is at least one in calcium dodecyl benzene sulfonate, alkylphenol polyoxyethylene phosphate, phenethyl phenol polyethenoxy ether phosphate etc., as emulsifier 500,601p, 602p etc., in addition, commercially available emulsifier production can also be used.
Wherein, solvent can adopt at least one in water, ethanol, isopropyl alcohol, n-butanol, ethyl acetate, butyl acetate, methyl-sulfoxide, turpentine oil, solvent naphtha, 1-METHYLPYRROLIDONE, dimethyl formamide etc.
Wherein, antifreeze can be glycerine, propane diols, ethylene glycol, sodium chloride, calcium chloride, sorbierite etc.
Wherein, defoamer is silicone defoaming agent etc.
Wherein, dispersant is at least one in lignosulfonates, PAA and propionamide copolymer, alkylnaphthalene sulfonate, polycarboxylic acid salt's condensation product, methyl sodiosul foaliphatate etc.
Wherein, wetting agent can be alkyl sulfate, alkylbenzenesulfonate, alkylnaphthalene sulfonate, aliphatic alcohol polyethenoxy base ether, alkylphenol-polyethenoxy base ether, sorbitan fatty acid ester etc.
Wherein, stabilizing agent can be 2-expoxy propane, butyl glycidyl ether, isopropyl alcohol, n-butanol, polyethylene glycol etc.
The composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract of the present invention can be prepared into any one formulation of applicable agricultural use by the method for this area routine, for the ease of storing and transport, can make microemulsion, soluble concentrate or aqua.
Further, the aqua be preferably made up of the component of following weight portion: Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, 8 parts of emulsifier, 602,5 parts of ethylene glycol, 47 parts of water; Wherein, Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract be 40 parts crude drug extract form.Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract can obtain for Fructus meliae toosendan and lettuce mix rear common extraction in proportion; Also can take Fructus meliae toosendan and lettuce in proportion, be mixed to get after extracting separately.
For the ease of storing and transport, extract can be concentrated, obtain concentrate, and then be prepared into various formulation, cycles of concentration can according to needs of production from Row sum-equal matrix, preferably, Fructus meliae toosendan is prepared from by the following method with Caulis et Folium Lactucae sativae extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
Further, the invention also discloses the preparation method that the present invention contains the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, it is made up of following steps:
A, extraction: take Fructus meliae toosendan particle and lettuce particle, mixing, adds solvent by solid-liquid ratio 1:3 ~ 12g/ml, and in 60 ~ 95 DEG C of refluxing extraction, filter, obtain extract, wherein, described solvent is organic solvent or water;
B, preparation: extract is made microemulsion, soluble concentrate or aqua.
Wherein, Fructus meliae toosendan particle and lettuce particle can adopt commercially available particle, conventional method also can be adopted to pulverize the acrial part such as Fructus meliae toosendan, lettuce haulm and obtain.In order to improve the effect of extraction, simultaneously cost-saving, Fructus meliae toosendan particle and lettuce particle cross 60 ~ 100 mesh sieves, preferred mistake 80 mesh sieve.
Further, in order to cost-saving, simultaneously also in order to improve extraction efficiency, the solid-liquid ratio in a step is preferably 1:4 ~ 10g/ml, is more preferably 1:6g/ml.Namely the Fructus meliae toosendan of lg and lettuce particle can add 3 ~ 12ml solvent extraction, preferably add 4 ~ 10ml, more preferably add 6ml.
Conventional organic solvent all can be used for the present invention, and in order to cost-saving, improve dissolution rate, ensure extraction efficiency, organic solvent is preferably methyl alcohol, ethanol, ethyl acetate, acetone, chloroform simultaneously, is more preferably 95%v/v ethanol.
In order to shorten preparation time, accelerate extraction rate, can stir when adding hot reflux, mixing speed is preferably 80 ~ 100 revs/min.
Further, reflux temperature is preferably 75 ~ 85 DEG C, is more preferably 82 DEG C; Reflow's cycle is twice, and each return time is 1.5 hours, and merging filtrate, obtains extract.
The present invention also provides the composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract of the present invention preventing and treating the application in crop pest.The composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract of the present invention, to lepidoptera pest, especially Pyralidae, Sulfur butterfly, noctuid insect, as beet snout moth's larva, cabbage caterpillar, cotton bollworm etc. have good preventive effect.
In the agricultural production of reality, in order to improve composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract to the control efficiency of insect pest, the described composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract is preferably applied to crop pest initial stage of origination; Composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract of the present invention can be used by common method, sprays on crop blade face.Preferably, when using, the present invention is diluted with water to the activity component concentration of crude drug weighing scale as 10 ~ 100ppm containing the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, namely the composition pesticide that 1g crude drug is obtained after extracting, when using, be diluted with water to 10kg, then its activity component concentration equals 100ppm; Preferably being diluted to activity component concentration is further that 20 ~ 50ppm sprays in plant blade face, and so that water membrane to appear in blade face, not lower of liquid is advisable; Secondary dispenser is carried out, the preferred continuous administration of a season crop 2 ~ 3 times after being preferable over 3 ~ 5 days.
Composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract of the present invention is mixed by Sichuan chinaberry extract and Caulis et Folium Lactucae sativae extract, or jointly extracted by Fructus meliae toosendan and lettuce and form, its preventive effect is far away higher than the extract of single component, particularly extract common after Fructus meliae toosendan and lettuce mixing, owing to having solubilising, hydrotropy effect between Fructus meliae toosendan and lettuce, the composition pesticide insecticidal effect made after common extraction is more remarkable.
Compared with prior art, the composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract of the present invention has the following advantages:
1, the composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract of the present invention has synergistic function, obvious to the control efficiency of lepidoptera pest.
2, Fructus meliae toosendan of the present invention and lettuce mix after jointly extracting or extracting respectively, and the composition pesticide made can improve crop immune system ability while quick-acting tagging, and can significantly improve its vigor.
3, the present invention take Chinese herbal medicine as raw material, thus there is not Pesticide Residue, and insect is difficult to produce resistance; Be used alone compared with chemical source agricultural chemicals, environmentally friendly.
4, when the composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract of the present invention uses, valid density is low, with low cost.
Embodiment
The present invention contains the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, and its active component is extracted by the crude drug of following weight portion and forms: Fructus meliae toosendan 1 part, 0.5 ~ 8 part, lettuce.Wherein, active component is preferably extracted by the crude drug of following weight portion and forms: Fructus meliae toosendan 1 part, 2 ~ 5 parts, lettuce; Be preferably again and formed by the crude drug extraction of following weight portion: Fructus meliae toosendan 1 part, 3 ~ 4 parts, lettuce; Be more preferably and formed by the crude drug extraction of following weight portion: Fructus meliae toosendan 1 part, 4 parts, lettuce.
Wherein, the acrial parts such as the Fructus meliae toosendan that the present invention adopts and lettuce are Chinese herbal medicine, contain the activity of the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract in order to improve the present invention, the Fructus meliae toosendan that Fructus meliae toosendan is preferably dry, the lettuce haulm that lettuce is preferably dry.
The present invention contains the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, and after its active component can be mixed by crude drug Fructus meliae toosendan and lettuce, common extraction forms; Also first can be extracted separately by Fructus meliae toosendan and obtain Sichuan chinaberry extract, lettuce is extracted separately and obtains Caulis et Folium Lactucae sativae extract, then is mixed by two kinds of extracts.In order to improve the insecticidal properties of composition pesticide of the present invention, after its active component is preferably and is mixed by Fructus meliae toosendan and lettuce, common extraction forms.
Further, in order to improve the stability of the composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, of the present invention containing in the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract also comprises auxiliary agent, described auxiliary agent can adopt the usual auxiliaries in agricultural chemicals, as at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
Wherein, emulsifier can adopt at least one in non-ionic surface active agent and anion surfactant, described non-ionic surface active agent can be dibenzyl xenyl APEO, styryl phenyl APEO, styryl phenyl APEO, phenethyl phenyl polyoxyethylene one polyethenoxy ether, OPEO, alkylphenol polyoxyethylene sulfosuccinate, aliphatic acid polyethenoxy ether, fatty alcohol-polyoxyethylene ether, aliphatic amine polyoxyethylene ether, castor oil ethylene oxide adduct and derivative thereof, at least one in polyol fatty acid ester and ethylene oxide adduct etc. thereof, described anion surfactant is at least one in calcium dodecyl benzene sulfonate, alkylphenol polyoxyethylene phosphate, phenethyl phenol polyethenoxy ether phosphate etc., as emulsifier 500,601p, 602p etc., in addition, commercially available emulsifier production can also be used.
Wherein, solvent can adopt at least one in water, ethanol, isopropyl alcohol, n-butanol, ethyl acetate, butyl acetate, methyl-sulfoxide, turpentine oil, solvent naphtha, 1-METHYLPYRROLIDONE, dimethyl formamide etc.
Wherein, antifreeze can be glycerine, propane diols, ethylene glycol, sodium chloride, calcium chloride, sorbierite etc.
Wherein, defoamer is silicone defoaming agent etc.
Wherein, dispersant is at least one in lignosulfonates, PAA and propionamide copolymer, alkylnaphthalene sulfonate, polycarboxylic acid salt's condensation product, methyl sodiosul foaliphatate etc.
Wherein, wetting agent can be alkyl sulfate, alkylbenzenesulfonate, alkylnaphthalene sulfonate, aliphatic alcohol polyethenoxy base ether, alkylphenol-polyethenoxy base ether, sorbitan fatty acid ester etc.
Wherein, stabilizing agent can be 2-expoxy propane, butyl glycidyl ether, isopropyl alcohol, n-butanol, polyethylene glycol etc.
The composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract of the present invention can be prepared into any one formulation of applicable agricultural use by the method for this area routine, for the ease of storing and transport, can make microemulsion, soluble concentrate or aqua.
Further, the aqua be preferably made up of the component of following weight portion: Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, 8 parts of emulsifier, 602,5 parts of ethylene glycol, 47 parts of water; Wherein, Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract be 40 parts crude drug extract form.Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract can obtain for Fructus meliae toosendan and lettuce mix rear common extraction in proportion; Also can take Fructus meliae toosendan and lettuce in proportion, be mixed to get after extracting separately.
For the ease of storing and transport, extract can be concentrated, obtain concentrate, and then be prepared into various formulation, cycles of concentration can according to needs of production from Row sum-equal matrix, preferably, Fructus meliae toosendan is prepared from by the following method with Caulis et Folium Lactucae sativae extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
Further, the invention also discloses the preparation method that the present invention contains the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, it is made up of following steps:
A, extraction: take Fructus meliae toosendan particle and lettuce particle, mixing, adds solvent by solid-liquid ratio 1:3 ~ 12g/ml, and in 60 ~ 95 DEG C of refluxing extraction, filter, obtain extract, wherein, described solvent is organic solvent or water;
B, preparation: extract is made microemulsion, soluble concentrate or aqua.
Wherein, Fructus meliae toosendan particle and lettuce particle can adopt commercially available particle, conventional method also can be adopted to pulverize the acrial part such as Fructus meliae toosendan, lettuce haulm and obtain.In order to improve the effect of extraction, simultaneously cost-saving, Fructus meliae toosendan particle and lettuce particle cross 60 ~ 100 mesh sieves, preferred mistake 80 mesh sieve.
Further, in order to cost-saving, simultaneously also in order to improve extraction efficiency, the solid-liquid ratio in a step is preferably 1:4 ~ 10g/ml, is more preferably 1:6g/ml.Namely the Fructus meliae toosendan of lg and lettuce particle can add 3 ~ 12ml solvent extraction, preferably add 4 ~ 10ml, more preferably add 6ml.
Conventional organic solvent all can be used for the present invention, and in order to cost-saving, improve dissolution rate, ensure extraction efficiency, organic solvent is preferably methyl alcohol, ethanol, ethyl acetate, acetone, chloroform simultaneously, is more preferably 95%v/v ethanol.
In order to shorten preparation time, accelerate extraction rate, can stir when adding hot reflux, mixing speed is preferably 80 ~ 100 revs/min.
Further, reflux temperature is preferably 75 ~ 85 DEG C, is more preferably 82 DEG C; Reflow's cycle is twice, and each return time is 1.5 hours, and merging filtrate, obtains extract.
The present invention also provides the composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract of the present invention preventing and treating the application in crop pest.The composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract of the present invention, to lepidoptera pest, especially Pyralidae, Sulfur butterfly, noctuid insect, as beet snout moth's larva, cabbage caterpillar, cotton bollworm etc. have good preventive effect.
In the agricultural production of reality, in order to improve composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract to the control efficiency of insect pest, the described composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract is preferably applied to crop pest initial stage of origination; Composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract of the present invention can be used by common method, sprays on crop blade face.Preferably, when using, the present invention is diluted with water to the activity component concentration of crude drug weighing scale as 10 ~ 100ppm containing the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, namely the composition pesticide that 1g crude drug is obtained after extracting, when using, be diluted with water to 10kg, then its activity component concentration equals 100ppm; Preferably being diluted to activity component concentration is further that 20 ~ 50ppm sprays in plant blade face, and so that water membrane to appear in blade face, not lower of liquid is advisable; Secondary dispenser is carried out, the preferred continuous administration of a season crop 2 ~ 3 times after being preferable over 3 ~ 5 days.
Composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract of the present invention is mixed by Sichuan chinaberry extract and Caulis et Folium Lactucae sativae extract, or jointly extracted by Fructus meliae toosendan and lettuce and form, its preventive effect is far away higher than the extract of single component, particularly extract common after Fructus meliae toosendan and lettuce mixing, owing to having solubilising, hydrotropy effect between Fructus meliae toosendan and lettuce, the composition pesticide insecticidal effect made after common extraction is more remarkable.
Below in conjunction with embodiment, the specific embodiment of the present invention is further described, does not therefore limit the present invention among described scope of embodiments.
Embodiment 1 contains the preparation of the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract
A, extraction: take dry Fructus meliae toosendan and lettuce haulm, mixing, pulverizes, and crosses 80 mesh sieves, adds extraction solution, refluxing extraction twice, filters, merging filtrate, concentrated, makes extract weight equal the weight of crude drug, obtains extract cy-01 ~ cy-06; Wherein, each parameter is in table 1;
B, preparation: in extract cy-01 ~ cy-06, add auxiliary agent in table 2, stir, must contain the composition pesticide CY-01 ~ CY-06 of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract.
The preparation technology parameter of table 1 Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract
Continued 1
| cy-03 | 1 | 4 | 1:6 | 95%v/v ethanol | 82 | 1.5 |
| cy-04 | 1 | 3 | 1:3 | Water | 95 | 3 |
| cy-05 | 1 | 5 | 1:8 | Methyl alcohol | 75 | 2 |
| cy-06 | 1 | 8 | 1:4 | Chloroform | 60 | 1 |
Wherein, Fructus meliae toosendan and lettuce haulm are all purchased from Anguo City Zhong Tian prepared slices of Chinese crude drugs Co., Ltd.
Table 2 contains the formula of the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract
Embodiment 2 contains the preparation of the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract
A, extraction: take dry Fructus meliae toosendan, pulverize, and crosses 80 mesh sieves, add extraction solution, refluxing extraction twice, filters, merging filtrate, and concentrated, make extract weight equal the weight of crude drug, obtain extract d1-01 ~ d1-03, wherein, each parameter is in table 3;
Take dry lettuce haulm, pulverize, cross 80 mesh sieves, add extraction solution, refluxing extraction twice, filter, merging filtrate, concentrated, make extract weight equal the weight of crude drug, obtain extract d2-01 ~ d2-03, wherein, each parameter is in table 4;
B, preparation: take Fructus meliae toosendan extract, lettuce extract and auxiliary agent, stirring and evenly mixing, obtain the composition pesticide CY-07 ~ CY-12 containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, wherein, each parameter is in table 5.
Table 3
Table 4
Table 5
Continued 5
Test example 1 the present invention contains the efficiency test of the composition pesticide tea tree tea geometrid of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract
1, test material
1.1 for studying thing and object: tea tree tea geometrid
1.2 reagent agent
Product in embodiment: CY-01 ~ CY-12;
Fructus meliae toosendan single dose (D1), lettuce single dose (D2);
Chemical reagent contrasts: gamma cyhalothrin (CK-01), purchased from Chengdu Kelilong Biochemical Co., Ltd.;
Clear water contrast (CK-02).
Wherein, the preparation method of Fructus meliae toosendan single dose is: the d1-03 getting 40g, and add 8g emulsifier 602,5g ethylene glycol, 47g water, stirring and evenly mixing, to obtain final product.
The preparation method of lettuce single dose is: the d2-03 getting 40g, and add 8g emulsifier 602,5g ethylene glycol, 47g water, stirring and evenly mixing, to obtain final product.
2, experimental scheme and method
This test 12 medicaments, 2 single dose contrasts, 1 contrast medicament, 1 blank, repeats for three times, Gong48Ge community.Minizone and surrounding all leave isolation strip, plot area 15m
2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the tea geometrid quantity statistics of blade before the test and listing mark.Within 5 days after dispenser, investigate and record the tea geometrid number alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
3, result of the test
Each group is repeated meter of averaging, and calculate Revision insect recluced rate and preventive effect, it the results are shown in Table 6.
Table 6
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| CY-01 | 489 | 130 | 73.42 | 76.38 |
| CY-02 | 457 | 110 | 75.93 | 78.61 |
| CY-03 | 468 | 81 | 82.69 | 84.62 |
| CY-04 | 424 | 85 | 79.95 | 82.19 |
| CY-05 | 416 | 96 | 76.92 | 79.50 |
| CY-06 | 445 | 114 | 74.38 | 77.24 8 --> |
| CY-07 | 486 | 184 | 62.14 | 66.36 |
| CY-08 | 493 | 173 | 64.91 | 68.82 |
| CY-09 | 474 | 137 | 71.10 | 74.32 |
| CY-10 | 465 | 146 | 68.60 | 72.10 |
| CY-11 | 405 | 137 | 66.17 | 69.95 |
| CY-12 | 449 | 154 | 65.70 | 69.53 |
| D1 | 467 | 304 | 34.90 | 42.16 |
| D2 | 481 | 462 | 3.95 | 14.66 |
| CK-01 | 484 | 73 | 84.92 | 86.60 |
| CK-02 | 454 | 511 | -12.56 | —— |
As can be seen from Table 6, product of the present invention has good preventive effect to tea geometrid, and better, visible than the control efficiency of single Sichuan chinaberry extract and Caulis et Folium Lactucae sativae extract, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.In addition, have no during dispenser and have poisoning to show to crop.
Test example 2 the present invention contains the efficiency test of the composition pesticide cabbage pieris of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract
Products C Y-01 of the present invention ~ CY-12 is used for preventing and treating cabbage pieris.This test 12 medicaments, 2 single doses, 1 blank, repeats for three times, Gong45Ge community.Minizone and surrounding all leave isolation strip, plot area 10m
2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the cabbage caterpillar quantity statistics alive of blade before the test and listing mark.Within 5 days after dispenser, investigate and record the cabbage caterpillar quantity alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group is repeated meter of averaging, and calculate Revision insect recluced rate and preventive effect, it the results are shown in Table 7.
Table 7
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| CY-01 | 433 | 95 | 78.06 | 81.50 |
| CY-02 | 526 | 88 | 83.27 | 85.90 |
| CY-03 | 486 | 64 | 86.83 | 88.90 |
| CY-04 | 454 | 68 | 85.02 | 87.37 |
| CY-05 | 419 | 72 | 82.82 | 85.51 |
| CY-06 | 465 | 99 | 78.71 | 82.05 |
| CY-07 | 448 | 158 | 64.73 | 70.27 9 --> |
| CY-08 | 432 | 130 | 69.91 | 74.63 |
| CY-09 | 416 | 112 | 73.08 | 77.30 |
| CY-10 | 499 | 139 | 72.14 | 76.52 |
| CY-11 | 501 | 147 | 70.66 | 75.27 |
| CY-12 | 503 | 148 | 70.58 | 75.20 |
| D1 | 528 | 343 | 35.04 | 45.24 |
| D2 | 467 | 448 | 4.07 | 19.13 |
| CK-02 | 553 | 656 | -18.63 | —— |
As can be seen from Table 7, product dialogue cabbage caterpillar of the present invention has good preventive effect, and better, visible than the control efficiency of single Sichuan chinaberry extract and Caulis et Folium Lactucae sativae extract, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.In addition, have no during dispenser and have poisoning to show to crop.
Test example 3 the present invention contains the efficiency test of the composition pesticide cucumber beet snout moth's larva of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract
Products C Y-01 of the present invention ~ CY-12 is used for prevent and treat cucumber beet snout moth's larva.This test 12 medicaments, 2 single doses, 1 blank, repeats for three times, Gong45Ge community.Minizone and surrounding all leave isolation strip, plot area 20m
2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the beet snout moth's larva quantity statistics alive of blade before the test and listing mark.Within 5 days after dispenser, investigate and record the beet snout moth's larva number alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group is repeated meter of averaging, and calculate Revision insect recluced rate and preventive effect, its result is as shown in table 8.
Table 8
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| CY-01 | 264 | 56 | 78.79 | 81.92 |
| CY-02 | 199 | 30 | 84.92 | 87.15 |
| CY-03 | 206 | 24 | 88.35 | 90.07 |
| CY-04 | 245 | 35 | 85.71 | 87.82 |
| CY-05 | 222 | 38 | 82.88 | 85.41 |
| CY-06 | 216 | 41 | 81.02 | 83.82 |
| CY-07 | 235 | 74 | 68.51 | 73.16 |
| CY-08 | 264 | 66 | 75.00 | 78.69 10 --> |
| CY-09 | 259 | 60 | 76.83 | 80.25 |
| CY-10 | 249 | 64 | 74.30 | 78.09 |
| CY-11 | 275 | 74 | 73.09 | 77.06 |
| CY-12 | 255 | 73 | 71.37 | 75.60 |
| D1 | 246 | 163 | 33.74 | 43.52 |
| D2 | 215 | 205 | 4.65 | 18.72 |
| CK-02 | 208 | 244 | -17.31 | —— |
As can be seen from Table 8, product of the present invention has good preventive effect to beet snout moth's larva, and better, visible than the control efficiency of single Sichuan chinaberry extract and Caulis et Folium Lactucae sativae extract, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.In addition, have no during dispenser and have poisoning to show to crop.
Test example 4 the present invention contains the efficiency test of the composition pesticide a variety of Chinese cabbage diamond-back moth of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract
Products C Y-01 of the present invention ~ CY-12 is used for preventing and treating a variety of Chinese cabbage diamond-back moth.This test 12 medicaments, 2 single doses, 1 blank, repeats for three times, Gong45Ge community.Minizone and surrounding all leave isolation strip, plot area 10m
2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the diamond-back moth quantity statistics alive of blade before the test and listing mark.Within 5 days after dispenser, investigate and record the diamond-back moth number alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group is repeated meter of averaging, and calculate Revision insect recluced rate and preventive effect, its result is as shown in table 9.
Table 9
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| CY-01 | 193 | 38 | 80.31 | 83.10 |
| CY-02 | 216 | 32 | 85.19 | 87.28 |
| CY-03 | 206 | 26 | 87.38 | 89.17 |
| CY-04 | 253 | 38 | 84.98 | 87.11 |
| CY-05 | 205 | 35 | 82.93 | 85.34 |
| CY-06 | 215 | 41 | 80.93 | 83.63 |
| CY-07 | 203 | 58 | 71.43 | 75.47 |
| CY-08 | 195 | 48 | 75.38 | 78.87 |
| CY-09 | 186 | 43 | 76.88 | 80.16 11 --> |
| CY-10 | 215 | 51 | 76.28 | 79.64 |
| CY-11 | 230 | 56 | 75.65 | 79.10 |
| CY-12 | 222 | 58 | 73.87 | 77.57 |
Continued 9
| D1 | 189 | 116 | 38.62 | 47.31 |
| D2 | 254 | 244 | 3.94 | 17.54 |
| CK-02 | 194 | 226 | -16.49 | —— |
As can be seen from Table 9, product of the present invention has good preventive effect to diamond-back moth, and better, visible than the control efficiency of single Sichuan chinaberry extract and Caulis et Folium Lactucae sativae extract, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.In addition, have no during dispenser and have poisoning to show to crop.
Claims (15)
1. contain the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, it is characterized in that, its active component is extracted by the crude drug of following weight portion and forms: Fructus meliae toosendan 1 part, 0.5 ~ 8 part, lettuce; Described extracting method is: take Fructus meliae toosendan particle and lettuce particle, and mixing, adds solvent by solid-liquid ratio 1:3 ~ 12g/ml, and in 60 ~ 95 DEG C of refluxing extraction, filter, obtain extract, wherein, described solvent is organic solvent or water; Described organic solvent is methyl alcohol, ethanol, ethyl acetate, acetone, chloroform.
2. the composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract according to claim 1, is characterized in that, its active component is extracted by the crude drug of following weight portion and forms: Fructus meliae toosendan 1 part, 2 ~ 5 parts, lettuce.
3. the composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract according to claim 2, is characterized in that, its active component is extracted by the crude drug of following weight portion and forms: Fructus meliae toosendan 1 part, 3 ~ 4 parts, lettuce.
4. the composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract according to claim 3, is characterized in that, its active component is extracted by the crude drug of following weight portion and forms: Fructus meliae toosendan 1 part, 4 parts, lettuce.
5. the composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract according to any one of Claims 1 to 4, is characterized in that: the described composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract is microemulsion, soluble concentrate or aqua.
6. the composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract according to any one of Claims 1 to 4, it is characterized in that: described containing in the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract also comprises auxiliary agent, and described auxiliary agent is at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
7. the composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract according to claim 5, it is characterized in that: described containing in the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract also comprises auxiliary agent, and described auxiliary agent is at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
8. the composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract according to claim 5, is characterized in that: described aqua is made up of the component of following weight portion: Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, 8 parts of emulsifier, 602,5 parts of ethylene glycol, 47 parts of water; Wherein, Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract be 40 parts crude drug extract form; Fructus meliae toosendan is prepared from by the following method with Caulis et Folium Lactucae sativae extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
9. the composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract according to claim 7, is characterized in that: described aqua is made up of the component of following weight portion: Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, 8 parts of emulsifier, 602,5 parts of ethylene glycol, 47 parts of water; Wherein, Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract be 40 parts crude drug extract form; Fructus meliae toosendan is prepared from by the following method with Caulis et Folium Lactucae sativae extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
10. prepare the method containing the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract described in any one of claim 1 ~ 9, it is characterized in that being made up of following steps:
A, extraction: take Fructus meliae toosendan particle and lettuce particle, mixing, adds solvent by solid-liquid ratio 1:3 ~ 12g/ml, and in 60 ~ 95 DEG C of refluxing extraction, filter, obtain extract, wherein, described solvent is organic solvent or water;
B, preparation: extract is made microemulsion, aqueous emulsion, soluble concentrate or aqua.
11. preparations according to claim 10 contain the method for the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, and it is characterized in that: in described a step, Fructus meliae toosendan particle and lettuce particle cross 60 ~ 100 mesh sieves; Solid-liquid ratio is 1:4 ~ 10g/ml; Organic solvent is methyl alcohol, ethanol, ethyl acetate, acetone, chloroform; Reflux temperature is 75 ~ 85 DEG C; Reflow's cycle is twice, and merging filtrate, obtains extract.
12. preparations according to claim 11 contain the method for the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, and it is characterized in that: in described a step, Fructus meliae toosendan particle and lettuce particle cross 80 mesh sieves; Solid-liquid ratio is 1:6g/ml; Organic solvent is 95%v/v ethanol; Reflux temperature is 82 DEG C.
The composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract described in 13. any one of claim 1 ~ 9 is preventing and treating the application in crop pest.
14. application of composition pesticide in control crop pest containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract according to claim 13, is characterized in that: described insect pest is lepidoptera pest; During application, be diluted with water to the activity component concentration of crude drug weighing scale as 10 ~ 100ppm.
15. application of composition pesticide in control crop pest containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract according to claim 14, is characterized in that: during application, are diluted with water to the activity component concentration of crude drug weighing scale as 20 ~ 50ppm.
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| CN106490017A (en) * | 2016-10-23 | 2017-03-15 | 张颂忠 | A kind of foliage fertilizer plant pesticide of preventing and treating tetranychus viennensis |
| CN107593781A (en) * | 2017-10-27 | 2018-01-19 | 张颂忠 | A kind of composition pesticide containing Caulis et Folium Lactucae sativae extract and methoxyfenozide |
| CN107646883A (en) * | 2017-10-27 | 2018-02-02 | 张锡林 | A kind of composition pesticide containing Caulis et Folium Lactucae sativae extract and fluoroform pyrrole ether |
| CN107624802A (en) * | 2017-10-27 | 2018-01-26 | 惠州市无龄康态健康科技有限公司 | A kind of composition pesticide containing Caulis et Folium Lactucae sativae extract and bromine cyanogen insect amide |
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