CN103704278B - Containing composition pesticide and the preparation method and application of Fructus meliae toosendan and triperygium wilfordii extractive - Google Patents
Containing composition pesticide and the preparation method and application of Fructus meliae toosendan and triperygium wilfordii extractive Download PDFInfo
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Abstract
The present invention relates to the composition pesticide containing Fructus meliae toosendan and triperygium wilfordii extractive and preparation method and application, belong to pesticide field.The technical problem to be solved in the present invention is to provide composition pesticide containing Fructus meliae toosendan and triperygium wilfordii extractive and preparation method and application.The composition pesticide containing Fructus meliae toosendan and triperygium wilfordii extractive of the present invention, its active component is extracted by the crude drug of following weight portion and forms: Fructus meliae toosendan 1 part, thunder godvine 0.2 ~ 4 part; After its active component can be mixed by crude drug Fructus meliae toosendan and thunder godvine, common extraction forms; Also Fructus meliae toosendan can be extracted separately and obtain Sichuan chinaberry extract, thunder godvine is extracted separately and obtains triperygium wilfordii extractive, then is mixed by two kinds of extracts.The present invention can be good at killing lepidopterous insects containing the composition pesticide of Fructus meliae toosendan and triperygium wilfordii extractive, has positive effect for agricultural production.
Description
Technical field
The present invention relates to the composition pesticide containing Fructus meliae toosendan and triperygium wilfordii extractive and preparation method and application, belong to pesticide field.
Background technology
Fructus meliae toosendan has another name called FRUCTUS TOOSENDAN, traditional Chinese medicine, is the ripening fruits of Meliaceae deciduous tree toosendanin.Mainly originate in the southern various places of China, superior with the product person in Sichuan, therefore have another name called Fructus meliae toosendan.Fructus meliae toosendan is cold in nature, bitter, is the one of qi-activating drug.Mainly enter Liver Channel, catharsis liver heat, promoting qi circulation and relieving pain, has the function except damp and hot, clearing away the liver-fire, pain relieving, desinsection, is that preparation is efficiently without the important source material of residual hazard free of contamination novel plant class agricultural chemicals.
Thunder godvine is lance section Thunder God platymiscium, bitter, pungent, cool in nature, greatly poison.Return liver, kidney channel.Effect is dispelled rheumatism, remove obstruction in channels to relieve pain, swelling and pain relieving, removing toxic substances desinsection.For damp and hot tubercle, the long-pending poison of cancer knurl, treat lepra reaction, rheumatoid arthritis etc. clinically with it, pharmacological research also shows that it has the effects such as antitumor, anti-inflammatory.Tripterygium root is containing wilfordine (wilfordine), wilforine (wilforine), Wilforgine (wilforgine), Wilfortrine and wilfortrine (wil-skin acid amides (celacinnine), celastrus orbiculatus furancarboxylic acid amine (celafurine), celastrus orbiculatus benzyl acid amides (celagbenzine), Triptolide (wilforlide) A, B, triptonoterpenol (triptonoterpenol), 16-hydroxytriptolide (16-hydroxytripto-lide), triptolide and triptolide (triptolide), table Triptolide triol (epitriptriolide), thunder kaurene lactone (tripterifordine), mapping-Lei kaurene lactone (antriptolactone), wilfordic acid (tripterygicacid), straight wedge oxalic acid (orthosphenicacid), cupreol (β-sitosterol) and Daucosterol (daucosterol), root xylem contains triptotriterpenoidal lactone A (triptoterpenoidlactoneA), Triptolide A, B, celastrin (celastrol) i.e. Celastrol (tripterine) etc.
Inventor finds through a large amount of experiments, thunder godvine and the common extract of Fructus meliae toosendan or by triperygium wilfordii extractive and Sichuan chinaberry extract mixing, insecticidal active ingredient wherein has synergistic function, can be good at killing lepidopterous insects, has positive effect for agricultural production.
Summary of the invention
The technical problem to be solved in the present invention is to provide composition pesticide containing Fructus meliae toosendan and triperygium wilfordii extractive and preparation method and application.
The present invention contains the composition pesticide of Fructus meliae toosendan and triperygium wilfordii extractive, and its active component is extracted by the crude drug of following weight portion and forms: Fructus meliae toosendan 1 part, thunder godvine 0.2 ~ 4 part.Wherein, active component is preferably extracted by the crude drug of following weight portion and forms: Fructus meliae toosendan 1 part, thunder godvine 0.5 ~ 2 part; Be more preferably and formed by the crude drug extraction of following weight portion: Fructus meliae toosendan 1 part, thunder godvine 0.5 ~ 1.5 part; More preferably extracted by the crude drug of following weight portion and form: Fructus meliae toosendan 1 part, thunder godvine 1 part.
Wherein, the Fructus meliae toosendan that the present invention adopts and thunder godvine are Chinese herbal medicine, contain the activity of the composition pesticide of Fructus meliae toosendan and triperygium wilfordii extractive in order to improve the present invention, the Fructus meliae toosendan that Fructus meliae toosendan is preferably dry, the tripterygium root that thunder godvine is preferably dry.
The present invention contains the composition pesticide of Fructus meliae toosendan and triperygium wilfordii extractive, and after its active component can be mixed by crude drug Fructus meliae toosendan and thunder godvine, common extraction forms; Also first can be extracted separately by Fructus meliae toosendan and obtain Sichuan chinaberry extract, thunder godvine is extracted separately and obtains triperygium wilfordii extractive, then is mixed by two kinds of extracts.In order to improve the insecticidal properties of composition pesticide of the present invention, after its active component is preferably and is mixed by Fructus meliae toosendan and thunder godvine, common extraction forms.
Further, in order to improve the stability of the composition pesticide containing Fructus meliae toosendan and triperygium wilfordii extractive, of the present invention containing in the composition pesticide of Fructus meliae toosendan and triperygium wilfordii extractive also comprises auxiliary agent, described auxiliary agent can adopt the usual auxiliaries in agricultural chemicals, as at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
Wherein, emulsifier can adopt at least one in non-ionic surface active agent and anion surfactant, described non-ionic surface active agent can be dibenzyl xenyl APEO, styryl phenyl APEO, styryl phenyl APEO, phenethyl phenyl polyoxyethylene one polyethenoxy ether, OPEO, alkylphenol polyoxyethylene sulfosuccinate, aliphatic acid polyethenoxy ether, fatty alcohol-polyoxyethylene ether, aliphatic amine polyoxyethylene ether, castor oil ethylene oxide adduct and derivative thereof, at least one in polyol fatty acid ester and ethylene oxide adduct etc. thereof, described anion surfactant is at least one in calcium dodecyl benzene sulfonate, alkylphenol polyoxyethylene phosphate, phenethyl phenol polyethenoxy ether phosphate etc., as emulsifier 500,601p, 602p etc., in addition, commercially available emulsifier production can also be used.
Wherein, solvent can adopt at least one in water, ethanol, isopropyl alcohol, n-butanol, ethyl acetate, butyl acetate, methyl-sulfoxide, turpentine oil, solvent naphtha, 1-METHYLPYRROLIDONE, dimethyl formamide etc.
Wherein, antifreeze can be glycerine, propane diols, ethylene glycol, sodium chloride, calcium chloride, sorbierite etc.
Wherein, defoamer is silicone defoaming agent etc.; Described dispersant is at least one in lignosulfonates, PAA and propionamide copolymer, alkylnaphthalene sulfonate, polycarboxylic acid salt's condensation product, methyl sodiosul foaliphatate etc.
Wherein, wetting agent can be alkyl sulfate, alkylbenzenesulfonate, alkylnaphthalene sulfonate, aliphatic alcohol polyethenoxy base ether, alkylphenol-polyethenoxy base ether, sorbitan fatty acid ester etc.
Wherein, stabilizing agent can be 2-expoxy propane, butyl glycidyl ether, isopropyl alcohol, n-butanol, polyethylene glycol etc.
The composition pesticide containing Fructus meliae toosendan and triperygium wilfordii extractive of the present invention can be prepared into any one formulation of applicable agricultural use by the method for this area routine, for the ease of storing and transport, can make microemulsion, aqua or soluble concentrate.
Further, the soluble concentrate be preferably made up of the component of following weight portion: Fructus meliae toosendan and triperygium wilfordii extractive, 15 parts of emulsifier 1601,3 parts of emulsifier, 500,5 parts of dimethyl sulfoxide (DMSO)s, 47 parts of ethanol, wherein, Fructus meliae toosendan and triperygium wilfordii extractive be 30 weight portions crude drug extract form.
For the ease of storing and transport, extract can be concentrated, obtain concentrate, and then be prepared into various formulation, cycles of concentration can according to needs of production from Row sum-equal matrix, preferably, Fructus meliae toosendan is prepared from by the following method with triperygium wilfordii extractive: crude drug is through extracting and being concentrated into identical with crude drug weight.
Second technical problem that the present invention solves is to provide the method that preparation contains the composition pesticide of Fructus meliae toosendan and triperygium wilfordii extractive.
The preparation method containing the composition pesticide of Fructus meliae toosendan and triperygium wilfordii extractive of the present invention is made up of following steps:
A, extraction: take Fructus meliae toosendan particle and thunder godvine particle, mixing, adds solvent by solid-liquid ratio 1:3 ~ 12g/ml, and in 60 ~ 95 DEG C of refluxing extraction, filter, obtain extract, wherein, described solvent is organic solvent or water;
B, preparation: extract is made microemulsion, soluble concentrate or aqua.
Wherein, Fructus meliae toosendan particle and thunder godvine particle can adopt commercially available particle, and conventional method pulverizing Fructus meliae toosendan and tripterygium root also can be adopted to obtain.In order to improve the effect of extraction, simultaneously cost-saving, Fructus meliae toosendan particle and thunder godvine particle cross 60 ~ 100 mesh sieves, preferred mistake 80 mesh sieve.
Further, in order to cost-saving, simultaneously also in order to improve extraction efficiency, the solid-liquid ratio in a step is preferably 1:4 ~ 10g/ml, is more preferably 1:6g/ml.Namely the Fructus meliae toosendan of lg and thunder godvine particle can add 3 ~ 12ml solvent extraction, preferably add 4 ~ 10ml, more preferably add 6ml.
Conventional organic solvent all can be used for the present invention, and in order to cost-saving, improve dissolution rate, ensure extraction efficiency, organic solvent is preferably methyl alcohol, ethanol, ethyl acetate, acetone, chloroform simultaneously, is more preferably 95%v/v ethanol.
In order to shorten preparation time, accelerate extraction rate, can stir when adding hot reflux, mixing speed is preferably 80 ~ 100 revs/min.
Further, reflux temperature is preferably 75 ~ 85 DEG C, is more preferably 82 DEG C; Reflow's cycle is twice, and each return time is 2 hours, and merging filtrate, obtains extract.
The present invention also provides the composition pesticide containing Fructus meliae toosendan and triperygium wilfordii extractive of the present invention preventing and treating the application in crop pest.The composition pesticide containing Fructus meliae toosendan and triperygium wilfordii extractive of the present invention, to lepidoptera pest as cabbage caterpillar, diamond-back moth, prodenia litura etc. have good preventive effect.
In the agricultural production of reality, in order to improve composition pesticide containing Fructus meliae toosendan and triperygium wilfordii extractive to the control efficiency of insect pest, the described composition pesticide containing Fructus meliae toosendan and triperygium wilfordii extractive is preferably applied to insect pest of the plant initial stage of origination; Composition pesticide containing Fructus meliae toosendan and triperygium wilfordii extractive of the present invention can be used by common method, sprays on plant leaf surface.Preferably, when using, the present invention is diluted with water to the activity component concentration of crude drug weighing scale as 10 ~ 100ppm containing the composition pesticide of Fructus meliae toosendan and triperygium wilfordii extractive, namely the composition pesticide that 1g crude drug is obtained after extracting, when using, be diluted with water to 10kg, then its activity component concentration equals 100ppm; Preferably being diluted to active ingredient concentration is further that 20 ~ 50ppm sprays in plant blade face, and so that water membrane to appear in blade face, not lower of liquid is advisable; Secondary dispenser is carried out, the preferred continuous administration of a season crop 2 ~ 3 times after being preferable over 3-5 days.
Composition pesticide containing Fructus meliae toosendan and triperygium wilfordii extractive of the present invention is mixed by Sichuan chinaberry extract and triperygium wilfordii extractive, or jointly extracted by Fructus meliae toosendan and thunder godvine and form, its preventive effect is far away higher than the extract of single component, particularly extract common after Fructus meliae toosendan and thunder godvine mixing, owing to having solubilising, hydrotropy effect between Fructus meliae toosendan and thunder godvine, the composition pesticide insecticidal effect made after common extraction is more remarkable.
Compared with prior art, the composition pesticide containing Fructus meliae toosendan and triperygium wilfordii extractive of the present invention has the following advantages:
1, the composition pesticide containing Fructus meliae toosendan and triperygium wilfordii extractive of the present invention has synergistic function, obvious to the control efficiency of the insects such as Lepidoptera.
2, Fructus meliae toosendan of the present invention and thunder godvine mix after jointly extracting or extracting respectively, and the composition pesticide made can improve crop immune system ability while quick-acting tagging, and can significantly improve its vigor.
3, the present invention take Chinese herbal medicine as raw material, thus there is not Pesticide Residue, and insect is difficult to produce resistance; Be used alone compared with chemical source agricultural chemicals, environmentally friendly.
4, when the composition pesticide containing Fructus meliae toosendan and triperygium wilfordii extractive of the present invention uses, valid density is low, with low cost.
Embodiment
The present invention contains the composition pesticide of Fructus meliae toosendan and triperygium wilfordii extractive, and its active component is extracted by the crude drug of following weight portion and forms: Fructus meliae toosendan 1 part, thunder godvine 0.2 ~ 4 part.Wherein, active component is preferably extracted by the crude drug of following weight portion and forms: Fructus meliae toosendan 1 part, thunder godvine 0.5 ~ 2 part; Be more preferably and formed by the crude drug extraction of following weight portion: Fructus meliae toosendan 1 part, thunder godvine 0.5 ~ 1.5 part; More preferably extracted by the crude drug of following weight portion and form: Fructus meliae toosendan 1 part, thunder godvine 1 part.
Wherein, the Fructus meliae toosendan that the present invention adopts and thunder godvine are Chinese herbal medicine, contain the activity of the composition pesticide of Fructus meliae toosendan and triperygium wilfordii extractive in order to improve the present invention, the Fructus meliae toosendan that Fructus meliae toosendan is preferably dry, the tripterygium root that thunder godvine is preferably dry.
The present invention contains the composition pesticide of Fructus meliae toosendan and triperygium wilfordii extractive, and after its active component can be mixed by crude drug Fructus meliae toosendan and thunder godvine, common extraction forms; Also first can be extracted separately by Fructus meliae toosendan and obtain Sichuan chinaberry extract, thunder godvine is extracted separately and obtains triperygium wilfordii extractive, then is mixed by two kinds of extracts.In order to improve the insecticidal properties of composition pesticide of the present invention, after its active component is preferably and is mixed by Fructus meliae toosendan and thunder godvine, common extraction forms.
Further, in order to improve the stability of the composition pesticide containing Fructus meliae toosendan and triperygium wilfordii extractive, of the present invention containing in the composition pesticide of Fructus meliae toosendan and triperygium wilfordii extractive also comprises auxiliary agent, described auxiliary agent can adopt the usual auxiliaries in agricultural chemicals, as at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
Wherein, emulsifier can adopt at least one in non-ionic surface active agent and anion surfactant, described non-ionic surface active agent can be dibenzyl xenyl APEO, styryl phenyl APEO, styryl phenyl APEO, phenethyl phenyl polyoxyethylene one polyethenoxy ether, OPEO, alkylphenol polyoxyethylene sulfosuccinate, aliphatic acid polyethenoxy ether, fatty alcohol-polyoxyethylene ether, aliphatic amine polyoxyethylene ether, castor oil ethylene oxide adduct and derivative thereof, at least one in polyol fatty acid ester and ethylene oxide adduct etc. thereof, described anion surfactant is at least one in calcium dodecyl benzene sulfonate, alkylphenol polyoxyethylene phosphate, phenethyl phenol polyethenoxy ether phosphate etc., as emulsifier 500,601p, 602p etc., in addition, commercially available emulsifier production can also be used.
Wherein, solvent can adopt at least one in water, ethanol, isopropyl alcohol, n-butanol, ethyl acetate, butyl acetate, methyl-sulfoxide, turpentine oil, solvent naphtha, 1-METHYLPYRROLIDONE, dimethyl formamide etc.
Wherein, antifreeze can be glycerine, propane diols, ethylene glycol, sodium chloride, calcium chloride, sorbierite etc.
Wherein, defoamer is silicone defoaming agent etc.; Described dispersant is at least one in lignosulfonates, PAA and propionamide copolymer, alkylnaphthalene sulfonate, polycarboxylic acid salt's condensation product, methyl sodiosul foaliphatate etc.
Wherein, wetting agent can be alkyl sulfate, alkylbenzenesulfonate, alkylnaphthalene sulfonate, aliphatic alcohol polyethenoxy base ether, alkylphenol-polyethenoxy base ether, sorbitan fatty acid ester etc.
Wherein, stabilizing agent can be 2-expoxy propane, butyl glycidyl ether, isopropyl alcohol, n-butanol, polyethylene glycol etc.
The composition pesticide containing Fructus meliae toosendan and triperygium wilfordii extractive of the present invention can be prepared into any one formulation of applicable agricultural use by the method for this area routine, for the ease of storing and transport, can make microemulsion, aqua or soluble concentrate.
Further, the soluble concentrate be preferably made up of the component of following weight portion: Fructus meliae toosendan and triperygium wilfordii extractive, 15 parts of emulsifier 1601,3 parts of emulsifier, 500,5 parts of dimethyl sulfoxide (DMSO)s, 47 parts of ethanol, wherein, Fructus meliae toosendan and triperygium wilfordii extractive be 30 weight portions crude drug extract form.
For the ease of storing and transport, extract can be concentrated, obtain concentrate, and then be prepared into various formulation, cycles of concentration can according to needs of production from Row sum-equal matrix, preferably, Fructus meliae toosendan is prepared from by the following method with triperygium wilfordii extractive: crude drug is through extracting and being concentrated into identical with crude drug weight.
Second technical problem that the present invention solves is to provide the method that preparation contains the composition pesticide of Fructus meliae toosendan and triperygium wilfordii extractive.
The preparation method containing the composition pesticide of Fructus meliae toosendan and triperygium wilfordii extractive of the present invention is made up of following steps:
A, extraction: take Fructus meliae toosendan particle and thunder godvine particle, mixing, adds solvent by solid-liquid ratio 1:3 ~ 12g/ml, and in 60 ~ 95 DEG C of refluxing extraction, filter, obtain extract, wherein, described solvent is organic solvent or water;
B, preparation: extract is made microemulsion, soluble concentrate or aqua.
Wherein, Fructus meliae toosendan particle and thunder godvine particle can adopt commercially available particle, and conventional method pulverizing Fructus meliae toosendan and tripterygium root also can be adopted to obtain.In order to improve the effect of extraction, simultaneously cost-saving, Fructus meliae toosendan particle and thunder godvine particle cross 60 ~ 100 mesh sieves, preferred mistake 80 mesh sieve.
Further, in order to cost-saving, simultaneously also in order to improve extraction efficiency, the solid-liquid ratio in a step is preferably 1:4 ~ 10g/ml, is more preferably 1:6g/ml.Namely the Fructus meliae toosendan of lg and thunder godvine particle can add 3 ~ 12ml solvent extraction, preferably add 4 ~ 10ml, more preferably add 6ml.
Conventional organic solvent all can be used for the present invention, and in order to cost-saving, improve dissolution rate, ensure extraction efficiency, organic solvent is preferably methyl alcohol, ethanol, ethyl acetate, acetone, chloroform simultaneously, is more preferably 95%v/v ethanol.
In order to shorten preparation time, accelerate extraction rate, can stir when adding hot reflux, mixing speed is preferably 80 ~ 100 revs/min.
Further, reflux temperature is preferably 75 ~ 85 DEG C, is more preferably 82 DEG C; Reflow's cycle is twice, and each return time is 2 hours, and merging filtrate, obtains extract.
The present invention also provides the composition pesticide containing Fructus meliae toosendan and triperygium wilfordii extractive of the present invention preventing and treating the application in crop pest.The composition pesticide containing Fructus meliae toosendan and triperygium wilfordii extractive of the present invention, to lepidoptera pest as cabbage caterpillar, diamond-back moth, prodenia litura etc. have good preventive effect.
In the agricultural production of reality, in order to improve composition pesticide containing Fructus meliae toosendan and triperygium wilfordii extractive to the control efficiency of insect pest, the described composition pesticide containing Fructus meliae toosendan and triperygium wilfordii extractive is preferably applied to insect pest of the plant initial stage of origination; Composition pesticide containing Fructus meliae toosendan and triperygium wilfordii extractive of the present invention can be used by common method, sprays on plant leaf surface.Preferably, when using, the present invention is diluted with water to the activity component concentration of crude drug weighing scale as 10 ~ 100ppm containing the composition pesticide of Fructus meliae toosendan and triperygium wilfordii extractive, namely the composition pesticide that 1g crude drug is obtained after extracting, when using, be diluted with water to 10kg, then its activity component concentration equals 100ppm; Preferably being diluted to active ingredient concentration is further that 20 ~ 50ppm sprays in plant blade face, and so that water membrane to appear in blade face, not lower of liquid is advisable; Secondary dispenser is carried out, the preferred continuous administration of a season crop 2 ~ 3 times after being preferable over 3-5 days.
Composition pesticide containing Fructus meliae toosendan and triperygium wilfordii extractive of the present invention is mixed by Sichuan chinaberry extract and triperygium wilfordii extractive, or jointly extracted by Fructus meliae toosendan and thunder godvine and form, its preventive effect is far away higher than the extract of single component, particularly extract common after Fructus meliae toosendan and thunder godvine mixing, owing to having solubilising, hydrotropy effect between Fructus meliae toosendan and thunder godvine, the composition pesticide insecticidal effect made after common extraction is more remarkable.
Below in conjunction with embodiment, the specific embodiment of the present invention is further described, does not therefore limit the present invention among described scope of embodiments.
Embodiment 1 contains the preparation of the composition pesticide of Fructus meliae toosendan and triperygium wilfordii extractive
A, extraction: take dry Fructus meliae toosendan and tripterygium root, mixing, pulverizes, and crosses 80 mesh sieves, adds extraction solution, refluxing extraction twice, filters, merging filtrate, concentrated, makes extract weight equal the weight of crude drug, obtains extract cl-01 ~ cl-06; Wherein, each parameter is in table 1;
B, preparation: in extract cl-01 ~ cl-06, add auxiliary agent in table 2, stir, must contain the composition pesticide CL-01 ~ CL-06 of Fructus meliae toosendan and triperygium wilfordii extractive.
The preparation technology parameter of table 1 Fructus meliae toosendan and triperygium wilfordii extractive
Continued 1
| cl-02 | 1 | 0.5 | 1:10 | Ethyl acetate | 70 | 4 |
| cl-03 | 1 | 1 | 1:6 | 95%v/v ethanol | 82 | 2 |
| cl-04 | 1 | 1.5 | 1:3 | Water | 95 | 3 |
| cl-05 | 1 | 2 | 1:8 | Methyl alcohol | 75 | 2 |
| cl-06 | 1 | 4 | 1:4 | Chloroform | 60 | 1 |
Wherein, Fructus meliae toosendan and tripterygium root are all from Anguo City Zhong Tian prepared slices of Chinese crude drugs Co., Ltd.
Table 2 contains the formula of the composition pesticide of Fructus meliae toosendan and triperygium wilfordii extractive
Embodiment 2 contains the preparation of the composition pesticide of Fructus meliae toosendan and triperygium wilfordii extractive
A, extraction: take dry Fructus meliae toosendan, pulverize, and crosses 80 mesh sieves, add extraction solution, refluxing extraction twice, filters, merging filtrate, and concentrated, make extract weight equal the weight of crude drug, obtain extract d1-01 ~ d1-03, wherein, each parameter is in table 3;
Take dry tripterygium root, pulverize, cross 80 mesh sieves, add extraction solution, refluxing extraction twice, filter, merging filtrate, concentrated, make extract weight equal the weight of crude drug, obtain extract d2-01 ~ d2-03, wherein, each parameter is in table 4;
B, preparation: take Fructus meliae toosendan extract, thunder godvine extract and auxiliary agent, stirring and evenly mixing, obtain the composition pesticide CL-07 ~ CL-12 containing Fructus meliae toosendan and triperygium wilfordii extractive, wherein, each parameter is in table 5.
Table 3
Table 4
Table 5
Test example 1 the present invention contains the efficiency test of the composition pesticide tea tree tea geometrid of Fructus meliae toosendan and triperygium wilfordii extractive
1, test material
1.1 for studying thing and object: tea tree tea geometrid
1.2 reagent agent
Product in embodiment: CL-01 ~ CL-12;
Fructus meliae toosendan single dose (D1), thunder godvine single dose (D2);
Chemical reagent contrasts: 10% Methomyl imidacloprid wettable powder (CK-01), purchased from Hailir Pharmaceutical Group Co., Ltd.;
Clear water contrast (CK-02).
Wherein, the preparation method of Fructus meliae toosendan single dose is: the d1-03 of 40g, and add 15g emulsifier 1601,3g emulsifier 500,5g dimethyl sulfoxide (DMSO), 47g ethanol, stirring and evenly mixing, to obtain final product.
Thunder godvine single dose preparation method is: the d2-03 of 40g, and add 15g emulsifier 1601,3g emulsifier 500,5g dimethyl sulfoxide (DMSO), 47g ethanol, stirring and evenly mixing, to obtain final product.
2, experimental scheme and method
This test 12 medicaments, 2 single dose contrasts, 1 contrast medicament, 1 blank, repeats for three times, Gong48Ge community.Minizone and surrounding all leave isolation strip, plot area 12m
2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the tea geometrid quantity statistics of blade before the test and listing mark.Within 5 days after dispenser, investigate and record the tea geometrid number alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
3, result of the test
Each group is repeated meter of averaging, and calculate Revision insect recluced rate and preventive effect, it the results are shown in Table 6.
Table 6
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| CL-01 | 344 | 98 | 71.51 | 75.91 |
| CL-02 | 391 | 75 | 80.82 | 83.78 |
Continued 6
| CL-03 | 308 | 54 | 82.47 | 85.17 |
| CL-04 | 371 | 81 | 78.17 | 81.54 |
| CL-05 | 394 | 96 | 75.63 | 79.40 |
| CL-06 | 382 | 100 | 73.82 | 77.86 |
| CL-07 | 346 | 131 | 62.14 | 67.98 |
| CL-08 | 381 | 114 | 70.08 | 74.70 |
| CL-09 | 371 | 102 | 72.51 | 76.75 |
| CL-10 | 346 | 102 | 70.52 | 75.07 |
| CL-11 | 303 | 97 | 67.99 | 72.93 |
| CL-12 | 338 | 118 | 65.09 | 70.48 |
| D1 | 319 | 223 | 30.09 | 40.89 |
| D2 | 335 | 227 | 32.24 | 42.70 |
| CK-01 | 370 | 53 | 85.68 | 87.89 |
| CK-02 | 356 | 421 | -18.26 | —— |
As can be seen from Table 6, product of the present invention has good preventive effect to tea geometrid, and better, visible than the control efficiency of single Sichuan chinaberry extract and triperygium wilfordii extractive, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.In addition, have no during dispenser and have poisoning to show to plant.
Test example 2 the present invention contains the efficiency test of the composition pesticide cabbage pieris of Fructus meliae toosendan and triperygium wilfordii extractive
Products C L-01 of the present invention ~ CL-12 is used for preventing and treating cabbage pieris.This test 12 medicaments, 2 single doses, 1 blank, repeats for three times, Gong45Ge community.Minizone and surrounding all leave isolation strip, plot area 20m
2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the cabbage caterpillar quantity statistics alive of blade before the test and listing mark.Within 5 days after dispenser, investigate and record the dark greyish green borer population alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group is repeated meter of averaging, and calculate Revision insect recluced rate and preventive effect, its result is as shown in table 7.
Table 7
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| CL-01 | 268 | 63 | 76.49 | 80.07 |
| CL-02 | 237 | 47 | 80.17 | 83.19 |
| CL-03 | 266 | 45 | 83.08 | 85.66 |
| CL-04 | 271 | 56 | 79.34 | 82.48 |
| CL-05 | 283 | 60 | 78.80 | 82.03 |
| CL-06 | 220 | 49 | 77.73 | 81.12 |
| CL-07 | 209 | 74 | 64.59 | 69.98 |
| CL-08 | 246 | 75 | 69.51 | 74.15 |
| CL-09 | 274 | 78 | 71.53 | 75.87 |
| CL-10 | 261 | 80 | 69.35 | 74.02 |
| CL-11 | 237 | 77 | 67.51 | 72.46 |
| CL-12 | 216 | 73 | 66.20 | 71.35 |
| D1 | 212 | 143 | 32.55 | 42.82 |
| D2 | 293 | 193 | 34.13 | 44.16 |
| CK-02 | 206 | 243 | -17.96 | —— |
As can be seen from Table 7, product of the present invention has good preventive effect to cabbage caterpillar, and better, visible than the control efficiency of single Sichuan chinaberry extract and triperygium wilfordii extractive, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.In addition, have no during dispenser and have poisoning to show to plant.
Test example 3 the present invention contains the efficiency test of the composition pesticide cucumber beet snout moth's larva of Fructus meliae toosendan and triperygium wilfordii extractive
Products C L-01 of the present invention ~ CL-12 is used for prevent and treat cucumber beet snout moth's larva.This test 12 medicaments, 2 single doses, 1 blank, repeats for three times, Gong45Ge community.Minizone and surrounding all leave isolation strip, plot area 16m
2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the beet snout moth's larva quantity statistics alive of blade before the test and listing mark.Within 5 days after dispenser, investigate and record the beet snout moth's larva number alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group is repeated meter of averaging, and calculate Revision insect recluced rate and preventive effect, its result is as shown in table 8.
Table 8
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| CL-01 | 422 | 82 | 80.57 | 83.32 |
| CL-02 | 465 | 64 | 86.24 | 88.18 |
| CL-03 | 472 | 52 | 88.98 | 90.54 |
| CL-04 | 436 | 57 | 86.93 | 88.78 |
| CL-05 | 482 | 75 | 84.44 | 86.64 |
| CL-06 | 410 | 71 | 82.68 | 85.13 |
| CL-07 | 409 | 117 | 71.39 | 75.44 |
| CL-08 | 473 | 113 | 76.11 | 79.49 |
| CL-09 | 451 | 97 | 78.49 | 81.53 |
| CL-10 | 442 | 102 | 76.92 | 80.19 |
| CL-11 | 491 | 124 | 74.75 | 78.32 |
| CL-12 | 438 | 117 | 73.29 | 77.07 |
| D1 | 408 | 262 | 35.78 | 44.87 |
| D2 | 416 | 258 | 37.98 | 46.75 |
| CK-02 | 437 | 509 | -16.48 | —— |
As can be seen from Table 8, product of the present invention has good preventive effect to beet snout moth's larva, and better, visible than the control efficiency of single Sichuan chinaberry extract and triperygium wilfordii extractive, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.In addition, have no during dispenser and have poisoning to show to plant.
Test example 4 the present invention contains the efficiency test of the composition pesticide cotton cotton bollworm of Fructus meliae toosendan and triperygium wilfordii extractive
Products C L-01 of the present invention ~ CL-12 is used for preventing and treating cotton cotton bollworm.This test 12 medicaments, 2 single doses, 1 blank, repeats for three times, Gong45Ge community.Minizone and surrounding all leave isolation strip, plot area 10m
2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the cotton bollworm quantity statistics alive of blade before the test and listing mark.Within 5 days after dispenser, investigate and record the cotton boll borer population alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group is repeated meter of averaging, and calculate Revision insect recluced rate and preventive effect, its result is as shown in table 9.
Table 9
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| CL-01 | 291 | 68 | 76.63 | 80.76 |
| CL-02 | 264 | 45 | 82.95 | 85.97 |
| CL-03 | 218 | 34 | 84.40 | 87.16 |
| CL-04 | 267 | 46 | 82.77 | 85.82 |
| CL-05 | 206 | 40 | 80.58 | 84.01 |
| CL-06 | 232 | 50 | 78.45 | 82.26 |
| CL-07 | 216 | 77 | 64.35 | 70.65 |
| CL-08 | 231 | 65 | 71.86 | 76.83 |
| CL-09 | 286 | 75 | 73.78 | 78.41 |
| CL-10 | 292 | 84 | 71.23 | 76.32 |
| CL-11 | 271 | 83 | 69.37 | 74.78 |
| CL-12 | 267 | 88 | 67.04 | 72.86 |
| D1 | 249 | 159 | 36.14 | 47.43 |
| D2 | 260 | 160 | 38.46 | 49.33 |
| CK-02 | 219 | 266 | -21.46 | —— |
As can be seen from Table 9, product of the present invention has good preventive effect to cotton bollworm, and better, visible than the control efficiency of single Sichuan chinaberry extract and triperygium wilfordii extractive, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.In addition, have no during dispenser and have poisoning to show to plant.
Claims (5)
1. contain the composition pesticide of Fructus meliae toosendan and triperygium wilfordii extractive, it is characterized in that, the described composition pesticide containing Fructus meliae toosendan and triperygium wilfordii extractive is soluble concentrate, described soluble concentrate is made up of the component of following weight portion: Fructus meliae toosendan and triperygium wilfordii extractive, 15 parts of emulsifier, 1601,3 parts of emulsifier 500,5 parts of dimethyl sulfoxide (DMSO)s, 47 parts of ethanol, wherein, Fructus meliae toosendan and triperygium wilfordii extractive are that the crude drug extraction of 30 weight portions forms; Fructus meliae toosendan is prepared from by the following method with triperygium wilfordii extractive: crude drug is through extracting and being concentrated into identical with crude drug weight.
2. the preparation method containing the composition pesticide of Fructus meliae toosendan and triperygium wilfordii extractive according to claim 1, is characterized in that being made up of following steps:
A, extraction: take Fructus meliae toosendan particle and thunder godvine particle, mixing, adds solvent by solid-liquid ratio 1:6g/ml, and in 82 DEG C of refluxing extraction, filter, obtain extract, wherein, described solvent is 95%v/v ethanol;
B, preparation: extract is made soluble concentrate.
3. the composition pesticide containing Fructus meliae toosendan and triperygium wilfordii extractive according to claim 1 is preventing and treating the application in crop pest.
4. the application of composition pesticide in control crop pest containing Fructus meliae toosendan and triperygium wilfordii extractive according to claim 3, is characterized in that: described insect pest is lepidoptera pest; During application, be diluted with water to the activity component concentration of crude drug weighing scale as 10 ~ 100ppm.
5. the application of composition pesticide in control crop pest containing Fructus meliae toosendan and triperygium wilfordii extractive according to claim 4, is characterized in that: during application, is diluted with water to the activity component concentration of crude drug weighing scale as 20 ~ 50ppm.
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| CN1049441A (en) * | 1989-08-13 | 1991-02-27 | 潘志荣 | Rotenone-pyrethroids insecticide |
| CN102599201B (en) * | 2012-02-27 | 2013-11-06 | 湖北中烟工业有限责任公司 | Compound plant-derived pesticide and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1049441A (en) * | 1989-08-13 | 1991-02-27 | 潘志荣 | Rotenone-pyrethroids insecticide |
| CN102599201B (en) * | 2012-02-27 | 2013-11-06 | 湖北中烟工业有限责任公司 | Compound plant-derived pesticide and preparation method thereof |
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| Title |
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| 川棘素与青虫菌等农药混用对菜青虫增效作用的试验;赵善欢等;《昆虫学报》;19890501;第32卷(第2期);第158-164页 * |
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