CN103704284B - Containing composition pesticide and the preparation method and application of black false hellebore and rhizoma calami extract - Google Patents
Containing composition pesticide and the preparation method and application of black false hellebore and rhizoma calami extract Download PDFInfo
- Publication number
- CN103704284B CN103704284B CN201310738261.9A CN201310738261A CN103704284B CN 103704284 B CN103704284 B CN 103704284B CN 201310738261 A CN201310738261 A CN 201310738261A CN 103704284 B CN103704284 B CN 103704284B
- Authority
- CN
- China
- Prior art keywords
- black false
- false hellebore
- rhizoma calami
- extract
- rhizoma
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to the composition pesticide containing black false hellebore and rhizoma calami extract and preparation method and application, belong to pesticide field.The technical problem to be solved in the present invention is to provide composition pesticide containing black false hellebore and rhizoma calami extract and preparation method and application.The composition pesticide containing black false hellebore and rhizoma calami extract of the present invention, its active component is extracted by the crude drug of following weight portion and forms: black false hellebore 1 part, rhizoma calami 0.5-6 part; After its active component can be mixed by crude drug black false hellebore and rhizoma calami, common extraction forms; Also first can be extracted separately by black false hellebore and obtain black false hellebore extract, rhizoma calami is extracted separately and obtains rhizoma calami extract, then is mixed by two kinds of extracts.The composition pesticide containing black false hellebore and rhizoma calami extract of the present invention, obvious to the control efficiency of Homoptera, acarina pest, for agricultural production, there is positive effect.
Description
Technical field
The present invention relates to the composition pesticide containing black false hellebore and rhizoma calami extract and preparation method and application, belong to pesticide field.
Background technology
In recent years, in agriculture and forestry field, due to single drug and not Scientific Usage of Drugs, plant pest has produced pesticide resistance in various degree, and agriculture and forestry produce still with chemical pesticide for mainly to prevent and treat kind, cause the destruction increasingly of environmental pollution and ecotope.Along with the raising of people's living standard and the enhancing of health perception, the manufacture and exploit of biopesticide just progressively receives the concern of agricultural chemicals R&D worker, becomes the focus of agriculture and forestry sustainable development, simultaneously, also be environmental protect, the effective way of minimizing or delaying drug resistance and raising drug effect.Wherein, botanical pesticide has become one of focus of biopesticide research.
Black false hellebore, the plant of Liliaceae Veratrum.Containing multiple bioactive ingredients such as jervine, jervine, false jervine, Rubijevine, colchicin, germerine and veratroylzygadenines in black false hellebore, find after deliberation, black false hellebore can be eliminated the phlegm, emetic, desinsection.Can be used for the diseases such as the heap soil or fertilizer over and around the roots for the treatment of apoplexy phlegm, epilepsy, angioleucitis, malaria, mastitis, fracture, traumatic injury, favus of the scalp, scabies, also can be used for go out maggot, fly etc.
Rhizoma calami is perennial very water type herbaceous plant.Complete stool has special aroma.Containing chemical compositions such as asarone, cis-Methylisoeugenol, methyl eugenol, calamus enediol, calamus helicene ketone, acolamone, acorone, the large bull ketone of calamus, acorones, have and have one's ideas straightened out, reduce phlegm, stomach invigorating, the effects such as inducing resuscitation.Can be used for the diseases such as treatment epilepsy, phlegm febrile convulsion, chest abdominal distension are vexed, chronic bronchitis.
In test, the present inventor finds that black false hellebore and the common extract of rhizoma calami and black false hellebore extract and the mixing of rhizoma calami extract have good synergistic function, can insecticide be prepared into, have good preventive effect to aphid, scale insect, tetranychid, trialeurodes vaporariorum etc.
Summary of the invention
The technical problem to be solved in the present invention is to provide the composition pesticide and its preparation method and application containing black false hellebore and rhizoma calami extract.
The present invention contains the composition pesticide of black false hellebore and rhizoma calami extract, and its active component is extracted by the crude drug of following weight portion and forms: black false hellebore 1 part, rhizoma calami 0.5 ~ 6 part.Wherein, active component is preferably extracted by the crude drug of following weight portion and forms: black false hellebore 1 part, rhizoma calami 1 ~ 4 part; Be preferably again and formed by the crude drug extraction of following weight portion: black false hellebore 1 part, rhizoma calami 1 ~ 3 part; Be more preferably and formed by the crude drug extraction of following weight portion: black false hellebore 1 part, rhizoma calami 2 parts.
Wherein, the black false hellebore that the present invention adopts and rhizoma calami are Chinese herbal medicine, contain the activity of the composition pesticide of black false hellebore and rhizoma calami extract in order to improve the present invention, the black false hellebore rhizome that black false hellebore is preferably dry, the rhizoma calami rhizome that rhizoma calami is preferably dry.
The present invention contains the composition pesticide of black false hellebore and rhizoma calami extract, and after its active component can be mixed by crude drug black false hellebore and rhizoma calami, common extraction forms; Also first can be extracted separately by black false hellebore and obtain black false hellebore extract, rhizoma calami is extracted separately and obtains rhizoma calami extract, then is mixed by two kinds of extracts.In order to improve the insecticidal properties of composition pesticide of the present invention, after its active component is preferably and is mixed by black false hellebore and rhizoma calami, common extraction forms.
Further, in order to improve the stability of the composition pesticide containing black false hellebore and rhizoma calami extract, the present invention contains in the composition pesticide of black false hellebore and rhizoma calami extract and also comprises auxiliary agent, described auxiliary agent can adopt the usual auxiliaries in agricultural chemicals, as at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
Wherein, emulsifier can adopt at least one in non-ionic surface active agent and anion surfactant, described non-ionic surface active agent can be dibenzyl xenyl APEO, styryl phenyl APEO, styryl phenyl APEO, phenethyl phenyl polyoxyethylene one polyethenoxy ether, OPEO, alkylphenol polyoxyethylene sulfosuccinate, aliphatic acid polyethenoxy ether, fatty alcohol-polyoxyethylene ether, aliphatic amine polyoxyethylene ether, castor oil ethylene oxide adduct and derivative thereof, at least one in polyol fatty acid ester and ethylene oxide adduct etc. thereof, described anion surfactant is at least one in calcium dodecyl benzene sulfonate, alkylphenol polyoxyethylene phosphate, phenethyl phenol polyethenoxy ether phosphate etc., as emulsifier 500,601p, 602p etc., in addition, commercially available emulsifier production can also be used.
Wherein, solvent can adopt at least one in water, ethanol, isopropyl alcohol, n-butanol, ethyl acetate, butyl acetate, methyl-sulfoxide, turpentine oil, solvent naphtha, 1-METHYLPYRROLIDONE, dimethyl formamide etc.
Wherein, antifreeze can be glycerine, propane diols, ethylene glycol, sodium chloride, calcium chloride, sorbierite etc.
Wherein, defoamer is silicone defoaming agent etc.
Wherein, dispersant is at least one in lignosulfonates, PAA and propionamide copolymer, alkylnaphthalene sulfonate, polycarboxylic acid salt's condensation product, methyl sodiosul foaliphatate etc.
Wherein, wetting agent can be alkyl sulfate, alkylbenzenesulfonate, alkylnaphthalene sulfonate, aliphatic alcohol polyethenoxy base ether, alkylphenol-polyethenoxy base ether, sorbitan fatty acid ester etc.
Wherein, stabilizing agent can be 2-expoxy propane, butyl glycidyl ether, isopropyl alcohol, n-butanol, polyethylene glycol etc.
The present invention can be prepared into any one formulation of applicable agricultural use containing the composition pesticide of black false hellebore and rhizoma calami extract by the method for this area routine, for the ease of storing and transport, can make microemulsion, aqua or soluble concentrate.
Further, the aqua be preferably made up of the component of following weight portion: black false hellebore and rhizoma calami extract, 10 parts of emulsifier op-10,5 parts of dimethyl sulfoxide (DMSO)s, 5 parts of ethylene glycol, 30 parts of water, wherein, black false hellebore and rhizoma calami extract are that the crude drug extraction of 50 parts forms.
For the ease of storing and transport, extract can be concentrated, obtain concentrate, and then be prepared into various formulation, cycles of concentration can according to needs of production from Row sum-equal matrix, preferably, black false hellebore is prepared from by the following method with rhizoma calami extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
Second technical problem that the present invention will solve is to provide the method that preparation contains the composition pesticide of black false hellebore and rhizoma calami extract.
The preparation method that the present invention contains the composition pesticide of black false hellebore and rhizoma calami extract is made up of following steps:
A, extraction: take black false hellebore particle and rhizoma calami particle, mixing, adds solvent by solid-liquid ratio 1:3 ~ 12g/ml, and in 60 ~ 95 DEG C of refluxing extraction, filter, obtain extract, wherein, described solvent is organic solvent or water;
B, preparation: extract is made microemulsion, soluble concentrate or aqua.
Wherein, black false hellebore particle and rhizoma calami particle can adopt commercially available particle, and conventional method pulverizing black false hellebore rhizome and rhizoma calami rhizome also can be adopted to obtain.In order to improve the effect of extraction, simultaneously cost-saving, black false hellebore particle and rhizoma calami particle cross 60 ~ 100 mesh sieves, preferred mistake 80 mesh sieve.
Further, in order to cost-saving, simultaneously also in order to improve extraction efficiency, the solid-liquid ratio in a step is preferably 1:4 ~ 10g/ml, is more preferably 1:6g/ml.Namely the black false hellebore of lg and rhizoma calami particle can add 3 ~ 12ml solvent extraction, preferably add 4 ~ 10ml, more preferably add 6ml.
Conventional organic solvent all can be used for the present invention, and in order to cost-saving, improve dissolution rate, ensure extraction efficiency, organic solvent is preferably methyl alcohol, ethanol, ethyl acetate, acetone, chloroform simultaneously, is more preferably the ethanol that volume fraction is 95%.
In order to shorten preparation time, accelerate extraction rate, can stir when adding hot reflux, mixing speed is preferably 80 ~ 100 revs/min.
Further, reflux temperature is preferably 75 ~ 85 DEG C, is more preferably 82 DEG C; Reflow's cycle is twice, and each return time is 2.5 hours, and merging filtrate, obtains extract.
The application of the composition pesticide that the present invention also provides the present invention to contain black false hellebore and rhizoma calami extract in control crop pest.The present invention contains the composition pesticide of black false hellebore and rhizoma calami extract, to homoptera pest as aphid, scale insect, trialeurodes vaporariorum etc.; The preventive effect that acarina pest is as good in panonychus citri, Tetranychus cinnabarinus etc. have.
In the agricultural production of reality, in order to improve composition pesticide containing black false hellebore and rhizoma calami extract to the control efficiency of insect pest, the described composition pesticide containing black false hellebore and rhizoma calami extract is preferably applied to crop pest initial stage of origination; The present invention can use by common method containing the composition pesticide of black false hellebore and rhizoma calami extract, sprays on crop blade face.Preferably, when using, the present invention is diluted with water to the activity component concentration of crude drug weighing scale as 10 ~ 100ppm containing the composition pesticide of black false hellebore and rhizoma calami extract, namely the composition pesticide that 1g crude drug is obtained after extracting, when using, be diluted with water to 10kg, then its activity component concentration equals 100ppm.Preferably being diluted to activity component concentration is further that 20 ~ 50ppm sprays in plant blade face, and so that water membrane to appear in blade face, not lower of liquid is advisable; Secondary dispenser is carried out, the preferred continuous administration of a season crop 2 ~ 3 times after being preferable over 3 ~ 5 days.
The present invention is mixed by black false hellebore extract and rhizoma calami extract containing the composition pesticide of black false hellebore and rhizoma calami extract, or common extraction forms after being mixed by black false hellebore and rhizoma calami, its preventive effect is far away higher than the extract of single component, particularly extract common after black false hellebore and rhizoma calami mixing, owing to having solubilising, hydrotropy effect between black false hellebore and rhizoma calami, the composition pesticide insecticidal effect made after common extraction is more remarkable.
Compared with prior art, the present invention has the following advantages containing the composition pesticide of black false hellebore and rhizoma calami extract:
1, the present invention has synergistic function containing the composition pesticide of black false hellebore and rhizoma calami extract, obvious to the control efficiency of the insect such as Homoptera, Acarina.
2, black false hellebore of the present invention and rhizoma calami mix after jointly extracting or extracting respectively, and the composition pesticide made can improve crop immune system ability while quick-acting tagging, and can significantly improve its vigor.
3, the present invention take Chinese herbal medicine as raw material, thus there is not Pesticide Residue, and insect is difficult to produce resistance; Be used alone compared with chemical source agricultural chemicals, environmentally friendly.
4, when the present invention uses containing the composition pesticide of black false hellebore and rhizoma calami extract, valid density is low, with low cost.
Embodiment
The present invention contains the composition pesticide of black false hellebore and rhizoma calami extract, and its active component is extracted by the crude drug of following weight portion and forms: black false hellebore 1 part, rhizoma calami 0.5 ~ 6 part.Wherein, active component is preferably extracted by the crude drug of following weight portion and forms: black false hellebore 1 part, rhizoma calami 1 ~ 4 part; Be preferably again and formed by the crude drug extraction of following weight portion: black false hellebore 1 part, rhizoma calami 1 ~ 3 part; Be more preferably and formed by the crude drug extraction of following weight portion: black false hellebore 1 part, rhizoma calami 2 parts.
Wherein, the black false hellebore that the present invention adopts and rhizoma calami are Chinese herbal medicine, contain the activity of the composition pesticide of black false hellebore and rhizoma calami extract in order to improve the present invention, the black false hellebore rhizome that black false hellebore is preferably dry, the rhizoma calami rhizome that rhizoma calami is preferably dry.
The present invention contains the composition pesticide of black false hellebore and rhizoma calami extract, and after its active component can be mixed by crude drug black false hellebore and rhizoma calami, common extraction forms; Also first can be extracted separately by black false hellebore and obtain black false hellebore extract, rhizoma calami is extracted separately and obtains rhizoma calami extract, then is mixed by two kinds of extracts.In order to improve the insecticidal properties of composition pesticide of the present invention, after its active component is preferably and is mixed by black false hellebore and rhizoma calami, common extraction forms.
Further, in order to improve the stability of the composition pesticide containing black false hellebore and rhizoma calami extract, the present invention contains in the composition pesticide of black false hellebore and rhizoma calami extract and also comprises auxiliary agent, described auxiliary agent can adopt the usual auxiliaries in agricultural chemicals, as at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
Wherein, emulsifier can adopt at least one in non-ionic surface active agent and anion surfactant, described non-ionic surface active agent can be dibenzyl xenyl APEO, styryl phenyl APEO, styryl phenyl APEO, phenethyl phenyl polyoxyethylene one polyethenoxy ether, OPEO, alkylphenol polyoxyethylene sulfosuccinate, aliphatic acid polyethenoxy ether, fatty alcohol-polyoxyethylene ether, aliphatic amine polyoxyethylene ether, castor oil ethylene oxide adduct and derivative thereof, at least one in polyol fatty acid ester and ethylene oxide adduct etc. thereof, described anion surfactant is at least one in calcium dodecyl benzene sulfonate, alkylphenol polyoxyethylene phosphate, phenethyl phenol polyethenoxy ether phosphate etc., as emulsifier 500,601p, 602p etc., in addition, commercially available emulsifier production can also be used.
Wherein, solvent can adopt at least one in water, ethanol, isopropyl alcohol, n-butanol, ethyl acetate, butyl acetate, methyl-sulfoxide, turpentine oil, solvent naphtha, 1-METHYLPYRROLIDONE, dimethyl formamide etc.
Wherein, antifreeze can be glycerine, propane diols, ethylene glycol, sodium chloride, calcium chloride, sorbierite etc.
Wherein, defoamer is silicone defoaming agent etc.
Wherein, dispersant is at least one in lignosulfonates, PAA and propionamide copolymer, alkylnaphthalene sulfonate, polycarboxylic acid salt's condensation product, methyl sodiosul foaliphatate etc.
Wherein, wetting agent can be alkyl sulfate, alkylbenzenesulfonate, alkylnaphthalene sulfonate, aliphatic alcohol polyethenoxy base ether, alkylphenol-polyethenoxy base ether, sorbitan fatty acid ester etc.
Wherein, stabilizing agent can be 2-expoxy propane, butyl glycidyl ether, isopropyl alcohol, n-butanol, polyethylene glycol etc.
The present invention can be prepared into any one formulation of applicable agricultural use containing the composition pesticide of black false hellebore and rhizoma calami extract by the method for this area routine, for the ease of storing and transport, can make microemulsion, aqua or soluble concentrate.
Further, the aqua be preferably made up of the component of following weight portion: black false hellebore and rhizoma calami extract, 10 parts of emulsifier op-10,5 parts of dimethyl sulfoxide (DMSO)s, 5 parts of ethylene glycol, 30 parts of water, wherein, black false hellebore and rhizoma calami extract are that the crude drug extraction of 50 parts forms.
For the ease of storing and transport, extract can be concentrated, obtain concentrate, and then be prepared into various formulation, cycles of concentration can according to needs of production from Row sum-equal matrix, preferably, black false hellebore is prepared from by the following method with rhizoma calami extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
The preparation method that the present invention contains the composition pesticide of black false hellebore and rhizoma calami extract is made up of following steps:
A, extraction: take black false hellebore particle and rhizoma calami particle, mixing, adds solvent by solid-liquid ratio 1:3 ~ 12g/ml, and in 60 ~ 95 DEG C of refluxing extraction, filter, obtain extract, wherein, described solvent is organic solvent or water;
B, preparation: extract is made microemulsion, soluble concentrate or aqua.
Wherein, black false hellebore particle and rhizoma calami particle can adopt commercially available particle, and conventional method pulverizing black false hellebore rhizome and rhizoma calami rhizome also can be adopted to obtain.In order to improve the effect of extraction, simultaneously cost-saving, black false hellebore particle and rhizoma calami particle cross 60 ~ 100 mesh sieves, preferred mistake 80 mesh sieve.
Further, in order to cost-saving, simultaneously also in order to improve extraction efficiency, the solid-liquid ratio in a step is preferably 1:4 ~ 10g/ml, is more preferably 1:6g/ml.Namely the black false hellebore of lg and rhizoma calami particle can add 3 ~ 12ml solvent extraction, preferably add 4 ~ 10ml, more preferably add 6ml.
Conventional organic solvent all can be used for the present invention, and in order to cost-saving, improve dissolution rate, ensure extraction efficiency, organic solvent is preferably methyl alcohol, ethanol, ethyl acetate, acetone, chloroform simultaneously, is more preferably the ethanol that volume fraction is 95%.
In order to shorten preparation time, accelerate extraction rate, can stir when adding hot reflux, mixing speed is preferably 80 ~ 100 revs/min.
Further, reflux temperature is preferably 75 ~ 85 DEG C, is more preferably 82 DEG C; Reflow's cycle is twice, and each return time is 2.5 hours, and merging filtrate, obtains extract.
The present invention contains the composition pesticide of black false hellebore and rhizoma calami extract, to homoptera pest as aphid, scale insect, trialeurodes vaporariorum etc.; The preventive effect that acarina pest is as good in panonychus citri, Tetranychus cinnabarinus etc. have.
In the agricultural production of reality, in order to improve composition pesticide containing black false hellebore and rhizoma calami extract to the control efficiency of insect pest, the described composition pesticide containing black false hellebore and rhizoma calami extract is preferably applied to crop pest initial stage of origination; The present invention can use by common method containing the composition pesticide of black false hellebore and rhizoma calami extract, sprays on crop blade face.Preferably, when using, the present invention is diluted with water to the activity component concentration of crude drug weighing scale as 10 ~ 100ppm containing the composition pesticide of black false hellebore and rhizoma calami extract, namely the composition pesticide that 1g crude drug is obtained after extracting, when using, be diluted with water to 10kg, then its activity component concentration equals 100ppm.Preferably being diluted to activity component concentration is further that 20 ~ 50ppm sprays in plant blade face, and so that water membrane to appear in blade face, not lower of liquid is advisable; Secondary dispenser is carried out, the preferred continuous administration of a season crop 2 ~ 3 times after being preferable over 3 ~ 5 days.
The present invention is mixed by black false hellebore extract and rhizoma calami extract containing the composition pesticide of black false hellebore and rhizoma calami extract, or common extraction forms after being mixed by black false hellebore and rhizoma calami, its preventive effect is far away higher than the extract of single component, particularly extract common after black false hellebore and rhizoma calami mixing, owing to having solubilising, hydrotropy effect between black false hellebore and rhizoma calami, the composition pesticide insecticidal effect made after common extraction is more remarkable.
Below in conjunction with embodiment, the specific embodiment of the present invention is further described, does not therefore limit the present invention among described scope of embodiments.
Embodiment 1 contains the preparation of the composition pesticide of black false hellebore and rhizoma calami extract
A, extraction: take dry black false hellebore rhizome and rhizoma calami rhizome, mixing, pulverizes, and crosses 80 mesh sieves, adds extraction solution, refluxing extraction twice, filters, merging filtrate, concentrated, makes extract weight equal the weight of crude drug, obtains extract ls-01 ~ ls-06; Wherein, each parameter is in table 1;
B, preparation: in extract ls-01 ~ ls-06, add auxiliary agent in table 2, stir, must contain the composition pesticide LS-01 ~ LS-06 of black false hellebore and rhizoma calami extract.
The preparation technology parameter of table 1 black false hellebore and rhizoma calami extract
Wherein, black false hellebore rhizome and rhizoma calami rhizome are all purchased from Anguo City Zhong Tian prepared slices of Chinese crude drugs Co., Ltd.
Table 2 contains the formula of the composition pesticide of black false hellebore and rhizoma calami extract
Embodiment 2 contains the preparation of the composition pesticide of black false hellebore and rhizoma calami extract
A, extraction: take dry black false hellebore rhizome, pulverize, and crosses 80 mesh sieves, add extraction solution, refluxing extraction twice, filters, merging filtrate, and concentrated, make extract weight equal the weight of crude drug, obtain extract d1-01 ~ d1-03, wherein, each parameter is in table 3;
Take dry rhizoma calami rhizome, pulverize, cross 80 mesh sieves, add extraction solution, refluxing extraction twice, filter, merging filtrate, concentrated, make extract weight equal the weight of crude drug, obtain extract d2-01 ~ d2-03, wherein, each parameter is in table 4;
B, preparation: take black false hellebore extract, rhizoma calami extract and auxiliary agent, stirring and evenly mixing, obtain the composition pesticide LS-07 ~ LS-12 containing black false hellebore and rhizoma calami extract, wherein, each parameter is in table 5.
Table 3
Table 4
Table 5
Test example 1 the present invention contains the efficiency test of the composition pesticide eggplant two spotted spider mite of black false hellebore and rhizoma calami extract
For clearly putting into field efficacy situation containing the composition pesticide of black false hellebore and rhizoma calami extract, inventor has carried out field control effectiveness test to this medicament.
1, test material
1.1 for studying thing and object: eggplant two spotted spider mite
1.2 reagent agent
Product in embodiment: LS-01 ~ LS-12;
Black false hellebore single dose (D1), rhizoma calami single dose (D2);
Chemical reagent contrast (CK-01): 1.8% abamectin emulsifiable concentrate, purchased from Longdeng Chemical Co Ltd, Jiangsu;
Clear water contrast (CK-02).
Wherein, the preparation method of black false hellebore single dose is: the d1-03 getting 50g, adds 10g emulsifier op-10,5g dimethyl sulfoxide (DMSO), 5g ethylene glycol, 30g water, stirring and evenly mixing, obtained black false hellebore single dose.
The preparation method of rhizoma calami single dose is: the d2-03 getting 50g, adds 10g emulsifier op-10,5g dimethyl sulfoxide (DMSO), 5g ethylene glycol, 30g water, stirring and evenly mixing, obtained rhizoma calami single dose.
2, experimental scheme and method
This test 14 medicaments, 1 contrast medicament, 1 blank, repeats for three times, Gong48Ge community.Minizone and surrounding all leave isolation strip, and plot area 12m2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the two spotted spider mite quantity statistics of blade before the test and listing mark.Within 5 days after dispenser, investigate and record the two spotted spider mite number alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
3, result of the test
Within 5 days after dispenser, investigate and record the borer population alive on each blade, calculate Revision insect recluced rate and preventive effect, it the results are shown in Table 6.
Table 6
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| LS-01 | 304 | 103 | 66.12 | 72.61 |
| LS-02 | 319 | 93 | 70.85 | 76.44 |
| LS-03 | 328 | 87 | 73.48 | 78.56 |
| LS-04 | 318 | 91 | 71.38 | 76.87 |
| LS-05 | 327 | 98 | 70.03 | 75.78 |
| LS-06 | 324 | 107 | 66.98 | 73.31 |
| LS-07 | 331 | 154 | 53.47 | 62.39 |
| LS-08 | 325 | 130 | 60.00 | 67.67 |
| LS-09 | 342 | 128 | 62.57 | 69.75 |
Continued 6
| LS-10 | 337 | 134 | 60.24 | 67.86 |
| LS-11 | 345 | 150 | 56.52 | 64.86 |
| LS-12 | 341 | 153 | 55.13 | 63.73 |
| D1 | 304 | 248 | 18.42 | 34.06 |
| D2 | 307 | 277 | 9.77 | 27.07 |
| CK-01 | 318 | 81 | 74.53 | 79.41 |
| CK-02 | 312 | 386 | -23.72 | —— |
As can be seen from Table 6, product of the present invention has good preventive effect to two spotted spider mite, and better, visible than the control efficiency of single black false hellebore extract and rhizoma calami extract, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.
Test example 2 the present invention contains the efficiency test of the composition pesticide orange caterpillar of black false hellebore and rhizoma calami extract
Product LS-01 ~ LS-12 of the present invention is used for preventing and treating orange caterpillar.This test 12 medicaments, 2 single doses, 1 blank, repeats for three times, Gong45Ge community.Minizone and surrounding all leave isolation strip, and plot area 20m2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the Aphed population statistics alive also listing mark of blade before the test.Within 5 days after dispenser, investigate and record the aphid number alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group is repeated meter of averaging, and calculate Revision insect recluced rate and preventive effect, it the results are shown in Table 7.
Table 7
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| LS-01 | 389 | 73 | 81.23 | 84.38 |
| LS-02 | 379 | 51 | 86.54 | 88.80 |
| LS-03 | 418 | 52 | 87.56 | 89.64 |
| LS-04 | 427 | 61 | 85.71 | 88.11 |
| LS-05 | 371 | 61 | 83.56 | 86.31 |
| LS-06 | 385 | 68 | 82.34 | 85.29 |
| LS-07 | 382 | 109 | 71.47 | 76.24 |
| LS-08 | 399 | 92 | 76.94 | 80.80 |
| LS-09 | 426 | 95 | 77.70 | 81.43 |
| LS-10 | 437 | 104 | 76.20 | 80.19 |
| LS-11 | 414 | 105 | 74.64 | 78.88 |
| LS-12 | 431 | 115 | 73.32 | 77.79 |
| D1 | 372 | 264 | 29.03 | 40.91 |
| D2 | 369 | 310 | 15.99 | 30.05 |
| CK-02 | 368 | 442 | -20.11 | —— |
As can be seen from Table 7, product of the present invention has good preventive effect to aphid, and better, visible than the control efficiency of single black false hellebore extract and rhizoma calami extract, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.In addition, have no during dispenser and have poisoning to show to crop.
Test example 3 the present invention contains the efficiency test of the composition pesticide oranges and tangerines cottonycushion scale of black false hellebore and rhizoma calami extract
Product LS-01 ~ LS-12 of the present invention is used for preventing and treating oranges and tangerines cottonycushion scale.This test 12 medicaments, 2 single doses, 1 blank, repeats for three times, Gong45Ge community.Minizone and surrounding all leave isolation strip, and plot area 20m2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the cottonycushion scale quantity statistics alive of blade before the test and listing mark.Within 5 days after dispenser, investigate and record the cottonycushion scale number alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group is repeated meter of averaging, and calculate Revision insect recluced rate and preventive effect, its result is as shown in table 8.
Table 8
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| LS-01 | 261 | 79 | 69.73 | 72.93 |
| LS-02 | 274 | 70 | 74.45 | 77.15 |
| LS-03 | 297 | 67 | 77.44 | 79.82 |
| LS-04 | 306 | 79 | 74.18 | 76.91 |
| LS-05 | 211 | 58 | 72.51 | 75.41 |
| LS-06 | 268 | 79 | 70.52 | 73.63 |
| LS-07 | 265 | 103 | 61.13 | 65.24 |
| LS-08 | 244 | 80 | 67.21 | 70.68 |
| LS-09 | 237 | 76 | 67.93 | 71.32 |
| LS-10 | 262 | 87 | 66.79 | 70.30 |
| LS-11 | 275 | 96 | 65.09 | 68.78 |
| LS-12 | 284 | 103 | 63.73 | 67.56 |
| D1 | 260 | 201 | 22.69 | 30.86 |
| D2 | 218 | 188 | 13.76 | 22.87 |
| CK-02 | 288 | 322 | -11.81 | —— |
As can be seen from Table 8, product of the present invention has good preventive effect to cottonycushion scale, and better, visible than the control efficiency of single black false hellebore extract and rhizoma calami extract, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.In addition, have no during dispenser and have poisoning to show to crop.
Test example 4 the present invention contains the efficiency test of the composition pesticide panonychus citri of black false hellebore and rhizoma calami extract
Product LS-01 ~ LS-12 of the present invention is used for preventing and treating panonychus citri.This test 12 medicaments, 2 single doses, 1 blank, repeats for three times, Gong45Ge community.Minizone and surrounding all leave isolation strip, and plot area 20m2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the Panonychus citri quantity statistics alive of blade before the test and listing mark.Within 5 days after dispenser, investigate and record the Panonychus citri number alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group is repeated meter of averaging, and calculate Revision insect recluced rate and preventive effect, its result is as shown in table 9.
Table 9
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| LS-01 | 448 | 150 | 66.52 | 71.92 |
| LS-02 | 421 | 110 | 73.87 | 78.08 |
| LS-03 | 469 | 112 | 76.12 | 79.97 |
| LS-04 | 473 | 119 | 74.84 | 78.90 |
| LS-05 | 463 | 131 | 71.71 | 76.27 |
| LS-06 | 472 | 142 | 69.92 | 74.77 |
| LS-07 | 448 | 196 | 56.25 | 63.30 |
| LS-08 | 421 | 152 | 63.90 | 69.72 |
| LS-09 | 428 | 146 | 65.89 | 71.39 |
| LS-10 | 496 | 176 | 64.52 | 70.24 |
| LS-11 | 430 | 164 | 61.86 | 68.01 |
| LS-12 | 424 | 168 | 60.38 | 66.77 |
| D1 | 472 | 398 | 15.68 | 29.27 |
| D2 | 493 | 459 | 6.90 | 21.91 |
| CK-02 | 437 | 521 | -19.22 | —— |
As can be seen from Table 9, product of the present invention has good preventive effect to Panonychus citri, and better, visible than the control efficiency of single black false hellebore extract and rhizoma calami extract, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.In addition, have no during dispenser and have poisoning to show to crop.
Claims (25)
1. contain the composition pesticide of black false hellebore and rhizoma calami extract, it is characterized in that, its active component is extracted by the crude drug of following weight portion and forms: black false hellebore 1 part, rhizoma calami 0.5 ~ 6 part.
2. the composition pesticide containing black false hellebore and rhizoma calami extract according to claim 1, is characterized in that, its active component is extracted by the crude drug of following weight portion and forms: black false hellebore 1 part, rhizoma calami 1 ~ 4 part.
3. the composition pesticide containing black false hellebore and rhizoma calami extract according to claim 2, is characterized in that, its active component is extracted by the crude drug of following weight portion and forms: black false hellebore 1 part, rhizoma calami 1 ~ 3 part.
4. the composition pesticide containing black false hellebore and rhizoma calami extract according to claim 3, is characterized in that, its active component is extracted by the crude drug of following weight portion and forms: black false hellebore 1 part, rhizoma calami 2 parts.
5. the composition pesticide containing black false hellebore and rhizoma calami extract according to any one of Claims 1 to 4, is characterized in that: after its active component is mixed by black false hellebore and rhizoma calami, common extraction forms; Or extract by black false hellebore the black false hellebore extract, the rhizoma calami that obtain and extract the rhizoma calami extract obtained and mix.
6. the composition pesticide containing black false hellebore and rhizoma calami extract according to any one of Claims 1 to 4, is characterized in that: the described composition pesticide containing black false hellebore and rhizoma calami extract is microemulsion, aqua or soluble concentrate.
7. the composition pesticide containing black false hellebore and rhizoma calami extract according to claim 5, is characterized in that: the described composition pesticide containing black false hellebore and rhizoma calami extract is microemulsion, aqua or soluble concentrate.
8. the composition pesticide containing black false hellebore and rhizoma calami extract according to any one of Claims 1 to 4, it is characterized in that: described containing in the composition pesticide of black false hellebore and rhizoma calami extract also comprises auxiliary agent, and described auxiliary agent is at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
9. the composition pesticide containing black false hellebore and rhizoma calami extract according to claim 5, it is characterized in that: described containing in the composition pesticide of black false hellebore and rhizoma calami extract also comprises auxiliary agent, and described auxiliary agent is at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
10. the composition pesticide containing black false hellebore and rhizoma calami extract according to claim 6, it is characterized in that: described containing in the composition pesticide of black false hellebore and rhizoma calami extract also comprises auxiliary agent, and described auxiliary agent is at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
11. composition pesticides containing black false hellebore and rhizoma calami extract according to claim 7, it is characterized in that: described containing in the composition pesticide of black false hellebore and rhizoma calami extract also comprises auxiliary agent, and described auxiliary agent is at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
12. composition pesticides containing black false hellebore and rhizoma calami extract according to claim 6, it is characterized in that: described aqua is made up of the component of following weight portion: black false hellebore and rhizoma calami extract, 10 parts of emulsifier op-10,5 parts of dimethyl sulfoxide (DMSO)s, 5 parts of ethylene glycol, 30 parts of water, wherein, black false hellebore and rhizoma calami extract are that the crude drug extraction of 50 parts forms.
13. composition pesticides containing black false hellebore and rhizoma calami extract according to claim 7, it is characterized in that: described aqua is made up of the component of following weight portion: black false hellebore and rhizoma calami extract, 10 parts of emulsifier op-10,5 parts of dimethyl sulfoxide (DMSO)s, 5 parts of ethylene glycol, 30 parts of water, wherein, black false hellebore and rhizoma calami extract are that the crude drug extraction of 50 parts forms.
14. composition pesticides containing black false hellebore and rhizoma calami extract according to claim 10, it is characterized in that: described aqua is made up of the component of following weight portion: black false hellebore and rhizoma calami extract, 10 parts of emulsifier op-10,5 parts of dimethyl sulfoxide (DMSO)s, 5 parts of ethylene glycol, 30 parts of water, wherein, black false hellebore and rhizoma calami extract are that the crude drug extraction of 50 parts forms.
15. composition pesticides containing black false hellebore and rhizoma calami extract according to claim 11, it is characterized in that: described aqua is made up of the component of following weight portion: black false hellebore and rhizoma calami extract, 10 parts of emulsifier op-10,5 parts of dimethyl sulfoxide (DMSO)s, 5 parts of ethylene glycol, 30 parts of water, wherein, black false hellebore and rhizoma calami extract are that the crude drug extraction of 50 parts forms.
16. composition pesticides containing black false hellebore and rhizoma calami extract according to claim 12, is characterized in that: black false hellebore is prepared from by the following method with rhizoma calami extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
17. composition pesticides containing black false hellebore and rhizoma calami extract according to claim 13, is characterized in that: black false hellebore is prepared from by the following method with rhizoma calami extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
18. composition pesticides containing black false hellebore and rhizoma calami extract according to claim 14, is characterized in that: black false hellebore is prepared from by the following method with rhizoma calami extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
19. composition pesticides containing black false hellebore and rhizoma calami extract according to claim 15, is characterized in that: black false hellebore is prepared from by the following method with rhizoma calami extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
The method containing the composition pesticide of black false hellebore and rhizoma calami extract described in 20. any one of preparation claim 1 ~ 19, is characterized in that being made up of following steps:
A, extraction: take black false hellebore particle and rhizoma calami particle, mixing, adds solvent by solid-liquid ratio 1:3 ~ 12g/ml, and in 60 ~ 95 DEG C of refluxing extraction, filter, obtain extract, wherein, described solvent is organic solvent or water;
B, preparation: extract is made microemulsion, soluble concentrate or aqua.
21. preparations according to claim 20 contain the method for the composition pesticide of black false hellebore and rhizoma calami extract, and it is characterized in that: in described a step, black false hellebore particle and rhizoma calami particle cross 60 ~ 100 mesh sieves; Solid-liquid ratio is 1:4 ~ 10g/ml; Organic solvent is methyl alcohol, ethanol, ethyl acetate, acetone, chloroform; Reflux temperature is 75 ~ 85 DEG C; Reflow's cycle is twice, and merging filtrate, obtains extract.
22. preparations according to claim 21 contain the method for the composition pesticide of black false hellebore and rhizoma calami extract, and it is characterized in that: in described a step, black false hellebore particle and rhizoma calami particle cross 80 mesh sieves; Solid-liquid ratio is 1:6g/ml; Organic solvent to be volume fraction be 95% ethanol; Reflux temperature is 82 DEG C.
The composition pesticide containing black false hellebore and rhizoma calami extract described in 23. any one of claim 1 ~ 19 is preventing and treating the application in crop pest.
24. application of composition pesticide in control crop pest containing black false hellebore and rhizoma calami extract according to claim 23, is characterized in that: described insect pest is homoptera pest, acarina pest; During application, be diluted with water to the activity component concentration of crude drug weighing scale as 10 ~ 100ppm.
25. application of composition pesticide in control crop pest containing black false hellebore and rhizoma calami extract according to claim 24, is characterized in that: be diluted with water to the activity component concentration of crude drug weighing scale as 20 ~ 50ppm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310738261.9A CN103704284B (en) | 2013-12-27 | 2013-12-27 | Containing composition pesticide and the preparation method and application of black false hellebore and rhizoma calami extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310738261.9A CN103704284B (en) | 2013-12-27 | 2013-12-27 | Containing composition pesticide and the preparation method and application of black false hellebore and rhizoma calami extract |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103704284A CN103704284A (en) | 2014-04-09 |
| CN103704284B true CN103704284B (en) | 2015-08-19 |
Family
ID=50397958
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310738261.9A Active CN103704284B (en) | 2013-12-27 | 2013-12-27 | Containing composition pesticide and the preparation method and application of black false hellebore and rhizoma calami extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103704284B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106070377B (en) * | 2016-06-22 | 2018-12-11 | 北京联合大学 | A kind of jervine plant source compound fungicide and its application |
| CN106973954A (en) * | 2017-03-09 | 2017-07-25 | 海门市嘉富来农产品有限公司 | A kind of high efficacy pesticide for plant and preparation method thereof |
| CN114404548A (en) * | 2022-01-28 | 2022-04-29 | 深圳玉莱漫生物科技有限公司 | Antifungal transdermal absorption ointment |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1175741C (en) * | 2001-06-22 | 2004-11-17 | 丁小武 | Pure plant disease treatment insecticide and its producing method |
| CN100444735C (en) * | 2006-12-25 | 2008-12-24 | 南风云 | Plant insecticide and its preparing method |
| CN101810192A (en) * | 2010-03-11 | 2010-08-25 | 徐建军 | Pesticide mixing method capable of reducing pesticide residue in agricultural products |
-
2013
- 2013-12-27 CN CN201310738261.9A patent/CN103704284B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN103704284A (en) | 2014-04-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103651632B (en) | Containing the composition pesticide and its preparation method and application of Fructus meliae toosendan and black false hellebore extract | |
| CN103704281B (en) | Containing the composition pesticide and its preparation method and application of kuh-seng and tobacco extract | |
| CN103651608B (en) | Containing composition pesticide and the preparation method and application of kitchen garden and aconite extract | |
| CN103651605B (en) | Containing composition pesticide and the preparation method and application of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract | |
| CN103704284B (en) | Containing composition pesticide and the preparation method and application of black false hellebore and rhizoma calami extract | |
| CN103704278B (en) | Containing composition pesticide and the preparation method and application of Fructus meliae toosendan and triperygium wilfordii extractive | |
| CN102726459A (en) | Swainsonia salsula Taub. acaricide and its preparation method | |
| CN103636683B (en) | Pesticide composition containing radix sophorae flavescentis and fructus cnidii extracts as well as preparation method and application of pesticide composition | |
| CN103734204B (en) | Pesticide composite containing schizonepeta and tobacco extract, preparation method and application | |
| CN105284914A (en) | Pesticide composition containing cynanchum komarovii and sophora alopecuroide extract as well as preparation method and application thereof | |
| CN103651596B (en) | Containing composition pesticide and the preparation method and application of kuh-seng and Artabotrys odoratissimus extract | |
| CN103651633B (en) | Pesticide composition containing lightyellow sophora root and veratrum nigrum extracts, and preparation method and application thereof | |
| CN103651592B (en) | Containing composition pesticide and the preparation method and application of Dalmatian chrysanthemum and extract from Lantana camara | |
| CN103689002B (en) | Containing composition pesticide and the preparation method and application of lantana and Azolla imbricata extract | |
| CN103704285B (en) | Pesticide composition containing extracts of veratrum nigrum and mamee-apple and preparation method and application of pesticide combination | |
| CN103651597B (en) | Containing the agricultural chemicals and its production and use of acalypha copperleaf and Derris ellipta extract | |
| CN105284913A (en) | Pesticide composition containing sophora alopecuroide and vetchleaf sophora root extract as well as preparation method and application thereof | |
| CN103651595B (en) | Pesticide composition containing lightyellow sophora root and Caesalpinia crista extracts, and preparation method and application thereof | |
| CN103749490B (en) | Plant virus resistant agent and preparation method thereof | |
| CN105284915A (en) | Pesticide composition containing extracts of folium isatidis and thyme and preparation method and application thereof | |
| CN105340979A (en) | Pesticide composition containing chenopodium ambrosioides and common peganum seed extract as well as preparation method and application of pesticide composition | |
| CN105284967A (en) | Pesticide composition containing extracts of cymbopogon distans and raw gingers and preparation method and application thereof | |
| CN103651598B (en) | Containing the agricultural chemicals and its production and use of Annona reticulata and Derris ellipta extract | |
| CN105475379A (en) | Pesticide composition containing fructus aurantii immaturus extract and cinnamon extract, and preparation method and application thereof | |
| CN103651616B (en) | Containing the agricultural chemicals and its production and use of potato and Derris ellipta extract |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 611630 No. five, No. 35 Industrial Road, Heshan Town, Pujiang County, Sichuan, Chengdu Patentee after: Chengdu new Chaoyang Crop Science Co., Ltd Address before: 611630 No. five, No. 35 Industrial Road, Heshan Town, Pujiang County, Sichuan, Chengdu Patentee before: CHENGDU NEWSUN CROPSCIENCE Co.,Ltd. |
|
| CP01 | Change in the name or title of a patent holder |