CN103651596B - Containing composition pesticide and the preparation method and application of kuh-seng and Artabotrys odoratissimus extract - Google Patents
Containing composition pesticide and the preparation method and application of kuh-seng and Artabotrys odoratissimus extract Download PDFInfo
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Abstract
The present invention relates to the composition pesticide containing kuh-seng and Artabotrys odoratissimus extract and preparation method and application, belong to pesticide field.The technical problem to be solved in the present invention is to provide composition pesticide containing kuh-seng and Artabotrys odoratissimus extract and preparation method and application.The composition pesticide containing kuh-seng and Artabotrys odoratissimus extract of the present invention, its active component is extracted by the crude drug of following weight portion and forms: kuh-seng 1 part, Artabotrys odoratissimus 1-10 part; After its active component can be mixed by crude drug kuh-seng and Artabotrys odoratissimus, common extraction forms; Also first can be extracted separately by kuh-seng and obtain shrubby sophora extract, Artabotrys odoratissimus extracts separately and obtains Artabotrys odoratissimus extract, then is mixed by two kinds of extracts.The composition pesticide containing kuh-seng and Artabotrys odoratissimus extract of the present invention, obvious to the control efficiency of Homoptera, Acarina, thrips, lepidoptera pest, for agricultural production, there is positive effect.
Description
Technical field
The present invention relates to the composition pesticide containing kuh-seng and Artabotrys odoratissimus extract and preparation method and application, belong to pesticide field.
Background technology
In recent years, in agriculture and forestry field, due to single drug and not Scientific Usage of Drugs, plant pest has produced pesticide resistance in various degree, and agriculture and forestry produce still with chemical pesticide for mainly to prevent and treat kind, cause the destruction increasingly of environmental pollution and ecotope.Along with the raising of people's living standard and the enhancing of health perception, the manufacture and exploit of biopesticide just progressively receives the concern of agricultural chemicals R&D worker, becomes the focus of agriculture and forestry sustainable development, simultaneously, also be environmental protect, the effective way of minimizing or delaying drug resistance and raising drug effect.Wherein, botanical pesticide has become one of focus of biopesticide research.
Kuh-seng, the plant that pulse family kuh-seng belongs to.The multiple alkaloids such as sophocarpine, Sophoridine, Iosmatrine, counterfeit indigo leaf alkali are oxidized containing matrine, oxymatrine, N-in its root; Also containing multiple flavonoidss such as the new alcohol of kuh-seng, kuh-seng chalcone, kuh-seng alcohol, formoononetin, kurarinone, maackiain, kushenin, Xanthohumol, different fulvic acids; In addition, also containing triterpenoid saponin and quinones.Find after deliberation, kuh-seng has the effect of anti-inflammation, all has inhibitory action to Propiobacterium, staphylococcus aureus, Escherichia coli.In recent years, matrine in agriculturally extensive use, and has good control efficiency, is a kind of low toxicity, low-residual, environmental protection type agricultural chemical.
Artabotrys odoratissimus, have another name called the claws of a hawk, five pawls are blue, the claws of a hawk are blue, warbler pawl, shank are blue, claws of a hawk peach, for Annonaceae Artabotrys odoratissimus platymiscium, containing the chemical composition such as claws of a hawk element (as claws of a hawk element A, claws of a hawk element B etc.), liriodendrin, atherospermidine and luteanine in Artabotrys odoratissimus plant, there are some researches show, Artabotrys odoratissimus has effect of preventing malaria.
In test, the present inventor finds that kuh-seng and the common extract of Artabotrys odoratissimus and shrubby sophora extract and the mixing of Artabotrys odoratissimus extract have good synergistic function, can insecticide be prepared into, have good preventive effect to aphid, scale insect, tetranychid, aleyrodid, thrips, cabbage caterpillar, diamond-back moth, prodenia litura etc.
Summary of the invention
The technical problem to be solved in the present invention is to provide composition pesticide containing kuh-seng and Artabotrys odoratissimus extract and preparation method and application.
The present invention contains the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, and its active component is extracted by the crude drug of following weight portion and forms: kuh-seng 1 part, Artabotrys odoratissimus 1 ~ 10 part.Wherein, active component is preferably extracted by the crude drug of following weight portion and forms: kuh-seng 1 part, Artabotrys odoratissimus 3 ~ 8 parts; Be preferably again and formed by the crude drug extraction of following weight portion: kuh-seng 1 part, Artabotrys odoratissimus 3 ~ 6 parts; Be more preferably and formed by the crude drug extraction of following weight portion: kuh-seng 1 part, Artabotrys odoratissimus 5 parts.
Wherein, the kuh-seng that the present invention adopts and Artabotrys odoratissimus are Chinese herbal medicine, contain the activity of the composition pesticide of kuh-seng and Artabotrys odoratissimus extract in order to improve the present invention, the radix sophorae that kuh-seng is preferably dry, the claws of a hawk floral leaf that Artabotrys odoratissimus is preferably dry.
The present invention contains the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, and after its active component can be mixed by crude drug kuh-seng and Artabotrys odoratissimus, common extraction forms; Also first can be extracted separately by kuh-seng and obtain shrubby sophora extract, Artabotrys odoratissimus extracts separately and obtains Artabotrys odoratissimus extract, then is mixed by two kinds of extracts.In order to improve the insecticidal properties of composition pesticide of the present invention, after its active component is preferably and is mixed by kuh-seng and Artabotrys odoratissimus, common extraction forms.
Further, in order to improve the stability of the composition pesticide containing kuh-seng and Artabotrys odoratissimus extract, the present invention contains in the composition pesticide of kuh-seng and Artabotrys odoratissimus extract and also comprises auxiliary agent, described auxiliary agent can adopt the usual auxiliaries in agricultural chemicals, as at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
Wherein, emulsifier can adopt at least one in non-ionic surface active agent and anion surfactant, described non-ionic surface active agent can be dibenzyl xenyl APEO, styryl phenyl APEO, styryl phenyl APEO, phenethyl phenyl polyoxyethylene one polyethenoxy ether, OPEO, alkylphenol polyoxyethylene sulfosuccinate, aliphatic acid polyethenoxy ether, fatty alcohol-polyoxyethylene ether, aliphatic amine polyoxyethylene ether, castor oil ethylene oxide adduct and derivative thereof, at least one in polyol fatty acid ester and ethylene oxide adduct etc. thereof, described anion surfactant is at least one in calcium dodecyl benzene sulfonate, alkylphenol polyoxyethylene phosphate, phenethyl phenol polyethenoxy ether phosphate etc., as emulsifier 500,601p, 602p etc., in addition, commercially available emulsifier production can also be used.
Wherein, solvent can adopt at least one in water, ethanol, isopropyl alcohol, n-butanol, ethyl acetate, butyl acetate, methyl-sulfoxide, turpentine oil, solvent naphtha, 1-METHYLPYRROLIDONE, dimethyl formamide etc.
Wherein, antifreeze can be glycerine, propane diols, ethylene glycol, sodium chloride, calcium chloride, sorbierite etc.
Wherein, defoamer is silicone defoaming agent etc.
Wherein, dispersant is at least one in lignosulfonates, PAA and propionamide copolymer, alkylnaphthalene sulfonate, polycarboxylic acid salt's condensation product, methyl sodiosul foaliphatate etc.
Wherein, wetting agent can be alkyl sulfate, alkylbenzenesulfonate, alkylnaphthalene sulfonate, aliphatic alcohol polyethenoxy base ether, alkylphenol-polyethenoxy base ether, sorbitan fatty acid ester etc.
Wherein, stabilizing agent can be 2-expoxy propane, butyl glycidyl ether, isopropyl alcohol, n-butanol, polyethylene glycol etc.
The present invention can be prepared into any one formulation of applicable agricultural use containing the composition pesticide of kuh-seng and Artabotrys odoratissimus extract by the method for this area routine, for the ease of storing and transport, can make microemulsion, aqua or soluble concentrate.
Further, the microemulsion be preferably made up of the component of following weight portion: kuh-seng and Artabotrys odoratissimus extract, 15 parts of emulsifier op-10,3 parts of emulsifier, 500,5 parts of dimethyl sulfoxide (DMSO)s, 10 parts of ethanol, 5 parts of ethylene glycol, 22 parts of water, wherein, kuh-seng and Artabotrys odoratissimus extract are that the crude drug extraction of 40 parts forms.
For the ease of storing and transport, extract can be concentrated, obtain concentrate, and then be prepared into various formulation, cycles of concentration can according to needs of production from Row sum-equal matrix, preferably, kuh-seng is prepared from by the following method with Artabotrys odoratissimus extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
Second technical problem that the present invention will solve is to provide the method that preparation contains the composition pesticide of kuh-seng and Artabotrys odoratissimus extract.
The preparation method that the present invention contains the composition pesticide of kuh-seng and Artabotrys odoratissimus extract is made up of following steps:
A, extraction: take kuh-seng particle and Artabotrys odoratissimus particle, mixing, adds solvent by solid-liquid ratio 1:3 ~ 12g/ml, regulate pH to 5-7, in 60 ~ 100 DEG C of refluxing extraction, filter, obtain extract, wherein, described solvent is methyl alcohol, ethanol, ethyl acetate, acetone, chloroform or water;
B, preparation: extract is made microemulsion, soluble concentrate or aqua.
Wherein, kuh-seng particle and Artabotrys odoratissimus particle can adopt commercially available particle, and conventional method pulverizing radix sophorae and claws of a hawk floral leaf also can be adopted to obtain.In order to improve the effect of extraction, simultaneously cost-saving, kuh-seng particle and Artabotrys odoratissimus particle cross 60 ~ 100 mesh sieves, preferred mistake 80 mesh sieve.
Further, in order to cost-saving, simultaneously also in order to improve extraction efficiency, the solid-liquid ratio in a step is preferably 1:4 ~ 10g/ml, is more preferably 1:6g/ml.Namely the kuh-seng of lg and Artabotrys odoratissimus particle can add 3 ~ 12ml solvent extraction, preferably add 4 ~ 10ml, more preferably add 6ml.
Conventional organic solvent all can be used for the present invention, and in order to cost-saving, improve dissolution rate, ensure extraction efficiency, solvent is preferably purified water simultaneously, and reflux temperature is preferably 100 DEG C, and pH value is preferably 6.0.In order to cost-saving, regulate in preparation method of the present invention the material of pH be selected from mass fraction be 1 ~ 10% hydrochloric acid solution or mass fraction be the sodium hydroxide solution of 1 ~ 10%.
In order to shorten preparation time, accelerate extraction rate, can stir when adding hot reflux, mixing speed is preferably 80 ~ 100 revs/min.
Further, reflow's cycle is twice, and each return time is 2 hours, and merging filtrate, obtains extract.
The application of the composition pesticide that the present invention also provides the present invention to contain kuh-seng and Artabotrys odoratissimus extract in control crop pest.The present invention contains the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, to homoptera pest as aphid, scale insect, trialeurodes vaporariorum etc.; Acarina pest is as panonychus citri, Tetranychus cinnabarinus etc.; Thysanoptera is as thrips; Lepidoptera pest such as cabbage caterpillar, diamond-back moth, prodenia litura, beet snout moth's larva, tea tree tea geometrid etc. have good preventive effect.
In the agricultural production of reality, in order to improve composition pesticide containing kuh-seng and Artabotrys odoratissimus extract to the control efficiency of insect pest, the described composition pesticide containing kuh-seng and Artabotrys odoratissimus extract is preferably applied to crop pest initial stage of origination; The present invention can use by common method containing the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, sprays on crop blade face.Preferably, when using, the present invention is diluted with water to the activity component concentration of crude drug weighing scale as 10 ~ 100ppm containing the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, namely the composition pesticide that 1g crude drug is obtained after extracting, when using, be diluted with water to 10kg, then its activity component concentration equals 100ppm.Preferably being diluted to activity component concentration is further that 20 ~ 50ppm sprays in plant blade face, and so that water membrane to appear in blade face, not lower of liquid is advisable; Secondary dispenser is carried out, the preferred continuous administration of a season crop 2 ~ 3 times after being preferable over 3 ~ 5 days.
The present invention is mixed by shrubby sophora extract and Artabotrys odoratissimus extract containing the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, or jointly extracted by kuh-seng and Artabotrys odoratissimus and form, its preventive effect is far away higher than the extract of single component, particularly extract common after kuh-seng and Artabotrys odoratissimus mixing, owing to having solubilising, hydrotropy effect between kuh-seng and Artabotrys odoratissimus, the composition pesticide insecticidal effect made after common extraction is more remarkable.
Compared with prior art, the present invention has the following advantages containing the composition pesticide of kuh-seng and Artabotrys odoratissimus extract:
1, the present invention has synergistic function containing the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, obvious to the control efficiency of Homoptera, Acarina, thrips, lepidoptera pest.
2, kuh-seng of the present invention and Artabotrys odoratissimus mix after jointly extracting or extracting respectively, and the composition pesticide made can improve crop immune system ability while quick-acting tagging, and can significantly improve its vigor.
3, the present invention take Chinese herbal medicine as raw material, thus there is not Pesticide Residue, and insect is difficult to produce resistance; Be used alone compared with chemical source agricultural chemicals, environmentally friendly.
4, when the present invention uses containing the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, valid density is low, with low cost.
Embodiment
The present invention contains the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, and its active component is extracted by the crude drug of following weight portion and forms: kuh-seng 1 part, Artabotrys odoratissimus 1 ~ 10 part.Wherein, active component is preferably extracted by the crude drug of following weight portion and forms: kuh-seng 1 part, Artabotrys odoratissimus 3 ~ 8 parts; Be preferably again and formed by the crude drug extraction of following weight portion: kuh-seng 1 part, Artabotrys odoratissimus 3 ~ 6 parts; Be more preferably and formed by the crude drug extraction of following weight portion: kuh-seng 1 part, Artabotrys odoratissimus 5 parts.
Wherein, the kuh-seng that the present invention adopts and Artabotrys odoratissimus are Chinese herbal medicine, contain the activity of the composition pesticide of kuh-seng and Artabotrys odoratissimus extract in order to improve the present invention, the radix sophorae that kuh-seng is preferably dry, the claws of a hawk floral leaf that Artabotrys odoratissimus is preferably dry.
The present invention contains the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, and after its active component can be mixed by crude drug kuh-seng and Artabotrys odoratissimus, common extraction forms; Also first can be extracted separately by kuh-seng and obtain shrubby sophora extract, Artabotrys odoratissimus extracts separately and obtains Artabotrys odoratissimus extract, then is mixed by two kinds of extracts.In order to improve the insecticidal properties of composition pesticide of the present invention, after its active component is preferably and is mixed by kuh-seng and Artabotrys odoratissimus, common extraction forms.
Further, in order to improve the stability of the composition pesticide containing kuh-seng and Artabotrys odoratissimus extract, the present invention contains in the composition pesticide of kuh-seng and Artabotrys odoratissimus extract and also comprises auxiliary agent, described auxiliary agent can adopt the usual auxiliaries in agricultural chemicals, as at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
Wherein, emulsifier can adopt at least one in non-ionic surface active agent and anion surfactant, described non-ionic surface active agent can be dibenzyl xenyl APEO, styryl phenyl APEO, styryl phenyl APEO, phenethyl phenyl polyoxyethylene one polyethenoxy ether, OPEO, alkylphenol polyoxyethylene sulfosuccinate, aliphatic acid polyethenoxy ether, fatty alcohol-polyoxyethylene ether, aliphatic amine polyoxyethylene ether, castor oil ethylene oxide adduct and derivative thereof, at least one in polyol fatty acid ester and ethylene oxide adduct etc. thereof, described anion surfactant is at least one in calcium dodecyl benzene sulfonate, alkylphenol polyoxyethylene phosphate, phenethyl phenol polyethenoxy ether phosphate etc., as emulsifier 500,601p, 602p etc., in addition, commercially available emulsifier production can also be used.
Wherein, solvent can adopt at least one in water, ethanol, isopropyl alcohol, n-butanol, ethyl acetate, butyl acetate, methyl-sulfoxide, turpentine oil, solvent naphtha, 1-METHYLPYRROLIDONE, dimethyl formamide etc.
Wherein, antifreeze can be glycerine, propane diols, ethylene glycol, sodium chloride, calcium chloride, sorbierite etc.
Wherein, defoamer is silicone defoaming agent etc.
Wherein, dispersant is at least one in lignosulfonates, PAA and propionamide copolymer, alkylnaphthalene sulfonate, polycarboxylic acid salt's condensation product, methyl sodiosul foaliphatate etc.
Wherein, wetting agent can be alkyl sulfate, alkylbenzenesulfonate, alkylnaphthalene sulfonate, aliphatic alcohol polyethenoxy base ether, alkylphenol-polyethenoxy base ether, sorbitan fatty acid ester etc.
Wherein, stabilizing agent can be 2-expoxy propane, butyl glycidyl ether, isopropyl alcohol, n-butanol, polyethylene glycol etc.
The present invention can be prepared into any one formulation of applicable agricultural use containing the composition pesticide of kuh-seng and Artabotrys odoratissimus extract by the method for this area routine, for the ease of storing and transport, can make microemulsion, aqua or soluble concentrate.
Further, the microemulsion be preferably made up of the component of following weight portion: kuh-seng and Artabotrys odoratissimus extract, 15 parts of emulsifier op-10,3 parts of emulsifier, 500,5 parts of dimethyl sulfoxide (DMSO)s, 10 parts of ethanol, 5 parts of ethylene glycol, 22 parts of water, wherein, kuh-seng and Artabotrys odoratissimus extract are that the crude drug extraction of 40 parts forms.
For the ease of storing and transport, extract can be concentrated, obtain concentrate, and then be prepared into various formulation, cycles of concentration can according to needs of production from Row sum-equal matrix, preferably, kuh-seng is prepared from by the following method with Artabotrys odoratissimus extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
The preparation method that the present invention contains the composition pesticide of kuh-seng and Artabotrys odoratissimus extract is made up of following steps:
A, extraction: take kuh-seng particle and Artabotrys odoratissimus particle, mixing, adds solvent by solid-liquid ratio 1:3 ~ 12g/ml, regulate pH to 5-7, in 60 ~ 100 DEG C of refluxing extraction, filter, obtain extract, wherein, described solvent is methyl alcohol, ethanol, ethyl acetate, acetone, chloroform or water;
B, preparation: extract is made microemulsion, soluble concentrate or aqua.
Wherein, kuh-seng particle and Artabotrys odoratissimus particle can adopt commercially available particle, and conventional method pulverizing radix sophorae and claws of a hawk floral leaf also can be adopted to obtain.In order to improve the effect of extraction, simultaneously cost-saving, kuh-seng particle and Artabotrys odoratissimus particle cross 60 ~ 100 mesh sieves, preferred mistake 80 mesh sieve.
Further, in order to cost-saving, simultaneously also in order to improve extraction efficiency, the solid-liquid ratio in a step is preferably 1:4 ~ 10g/ml, is more preferably 1:6g/ml.Namely the kuh-seng of lg and Artabotrys odoratissimus particle can add 3 ~ 12ml solvent extraction, preferably add 4 ~ 10ml, more preferably add 6ml.
Conventional organic solvent all can be used for the present invention, and in order to cost-saving, improve dissolution rate, ensure extraction efficiency, solvent is preferably purified water simultaneously, and reflux temperature is preferably 100 DEG C, and pH value is preferably 6.0.In order to cost-saving, regulate in preparation method of the present invention the material of pH be selected from mass fraction be 1 ~ 10% hydrochloric acid solution or mass fraction be the sodium hydroxide solution of 1 ~ 10%.
In order to shorten preparation time, accelerate extraction rate, can stir when adding hot reflux, mixing speed is preferably 80 ~ 100 revs/min.
Further, reflow's cycle is twice, and each return time is 2 hours, and merging filtrate, obtains extract.
The present invention contains the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, to homoptera pest as aphid, scale insect, trialeurodes vaporariorum etc.; Acarina pest is as panonychus citri, Tetranychus cinnabarinus etc.; Thysanoptera is as thrips; Lepidoptera pest such as cabbage caterpillar, diamond-back moth, prodenia litura, beet snout moth's larva, tea tree tea geometrid etc. have good preventive effect.
In the agricultural production of reality, in order to improve composition pesticide containing kuh-seng and Artabotrys odoratissimus extract to the control efficiency of insect pest, the described composition pesticide containing kuh-seng and Artabotrys odoratissimus extract is preferably applied to crop pest initial stage of origination; The present invention can use by common method containing the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, sprays on crop blade face.Preferably, when using, the present invention is diluted with water to the activity component concentration of crude drug weighing scale as 10 ~ 100ppm containing the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, namely the composition pesticide that 1g crude drug is obtained after extracting, when using, be diluted with water to 10kg, then its activity component concentration equals 100ppm.Preferably being diluted to activity component concentration is further that 20 ~ 50ppm sprays in plant blade face, and so that water membrane to appear in blade face, not lower of liquid is advisable; Secondary dispenser is carried out, the preferred continuous administration of a season crop 2 ~ 3 times after being preferable over 3 ~ 5 days.
The present invention is mixed by shrubby sophora extract and Artabotrys odoratissimus extract containing the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, or jointly extracted by kuh-seng and Artabotrys odoratissimus and form, its preventive effect is far away higher than the extract of single component, particularly extract common after kuh-seng and Artabotrys odoratissimus mixing, owing to having solubilising, hydrotropy effect between kuh-seng and Artabotrys odoratissimus, the composition pesticide insecticidal effect made after common extraction is more remarkable.
Below in conjunction with embodiment, the specific embodiment of the present invention is further described, does not therefore limit the present invention among described scope of embodiments.
Embodiment 1 contains the preparation of the composition pesticide of kuh-seng and Artabotrys odoratissimus extract
A, extraction: take dry radix sophorae and claws of a hawk floral leaf, mixing, pulverizes, and crosses 80 mesh sieves, add extraction solution, adjust ph is 6.0, refluxing extraction twice, filters, merging filtrate, concentrated, make extract weight equal the weight of crude drug, obtain extract ky-01 ~ ky-06; Wherein, each parameter is in table 1;
B, preparation: in extract ky-01 ~ ky-06, add auxiliary agent in table 2, stir, must contain the composition pesticide KY-01 ~ KY-06 of kuh-seng and Artabotrys odoratissimus extract.
The preparation technology parameter of table 1 kuh-seng and Artabotrys odoratissimus extract
Wherein, radix sophorae and claws of a hawk floral leaf are all purchased from Anguo City Zhong Tian prepared slices of Chinese crude drugs Co., Ltd.
Table 2 contains the formula of the composition pesticide of kuh-seng and Artabotrys odoratissimus extract
Embodiment 2 contains the preparation of the composition pesticide of kuh-seng and Artabotrys odoratissimus extract
A, extraction: take dry radix sophorae, pulverize, and cross 80 mesh sieves, add extraction solution, adjust ph is 6.0, refluxing extraction twice, filters, merging filtrate, concentrated, make extract weight equal the weight of crude drug, obtain extract d1-01 ~ d1-03, wherein, each parameter is in table 3;
Take dry claws of a hawk floral leaf, pulverize, cross 80 mesh sieves, add extraction solution, adjust ph is 6.0, refluxing extraction twice, filters, merging filtrate, concentrated, make extract weight equal the weight of crude drug, obtain extract d2-01 ~ d2-03, wherein, each parameter is in table 4;
B, preparation: take Radix Sophorae Flavescentis extractive liquid, Artabotrys odoratissimus extract and auxiliary agent, stirring and evenly mixing, obtain the composition pesticide KY-07 ~ KY-12 containing kuh-seng and Artabotrys odoratissimus extract, wherein, each parameter is in table 5.
Table 3
Table 4
Table 5
Test example 1 the present invention contains the efficiency test of the composition pesticide wild cabbage diamond-back moth of kuh-seng and Artabotrys odoratissimus extract
For clearly putting into field efficacy situation containing the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, inventor carries out field control effectiveness test to this medicament.
1, test material
1.1 for studying thing and object: pumpkin aphid
1.2 reagent agent
Product in embodiment: KY-01 ~ KY-12;
Kuh-seng single dose (D1), Artabotrys odoratissimus single dose (D2):
Chemical reagent contrast (CK-01): 10% Methomyl Imidacloprid, purchased from Hailir Pharmaceutical Group Co., Ltd.;
Clear water contrast (CK-02).
Wherein, the preparation method of kuh-seng single dose is: the d1-03 getting 40g, adds 15g emulsifier op-10,3g emulsifier 500,5g dimethyl sulfoxide (DMSO), 10g ethanol, 5g ethylene glycol, 22g water, stirring and evenly mixing, obtained kuh-seng single dose.
The preparation method of Artabotrys odoratissimus single dose is: the d2-03 getting 40g, adds 15g emulsifier op-10,3g emulsifier 500,5g dimethyl sulfoxide (DMSO), 10g ethanol, 5g ethylene glycol, 22g water, stirring and evenly mixing, obtained Artabotrys odoratissimus single dose.
2, experimental scheme and method
This test 14 medicaments, 1 contrast medicament, 1 blank, repeats for three times, Gong48Ge community.Minizone and surrounding all leave isolation strip, plot area 12m
2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the Aphed population statistics alive also listing mark of blade before the test.Within 5 days after dispenser, investigate and record the aphid number alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
3, result of the test
Within 5 days after dispenser, investigate and record the borer population alive on each blade, calculate Revision insect recluced rate and preventive effect, it the results are shown in Table 6.
Table 6
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| KY-01 | 313 | 61 | 80.51 | 83.22 |
| KY-02 | 322 | 43 | 86.65 | 88.50 |
| KY-03 | 353 | 41 | 88.39 | 90.00 |
| KY-04 | 344 | 43 | 87.50 | 89.24 |
| KY-05 | 367 | 54 | 85.29 | 87.33 |
| KY-06 | 325 | 52 | 84.00 | 86.22 |
| KY-07 | 374 | 100 | 73.26 | 76.98 |
| KY-08 | 396 | 90 | 77.27 | 80.43 |
| KY-09 | 389 | 85 | 78.15 | 81.19 |
| KY-10 | 370 | 83 | 77.57 | 80.68 |
| KY-11 | 358 | 91 | 74.58 | 78.11 |
| KY-12 | 343 | 88 | 74.34 | 77.91 |
| D1 | 338 | 216 | 36.09 | 44.98 |
| D2 | 361 | 260 | 27.98 | 37.99 |
| CK-01 | 388 | 41 | 89.43 | 90.90 |
| CK-02 | 316 | 367 | -16.14 | —— |
As can be seen from Table 6, product of the present invention has good preventive effect to aphid, and better, visible than the control efficiency of single shrubby sophora extract and Artabotrys odoratissimus extract, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.
Test example 2 the present invention contains the efficiency test of the composition pesticide cucumber two spotted spider mite of kuh-seng and Artabotrys odoratissimus extract
Product K Y-01 of the present invention ~ KY-12 is used for preventing and treating cucumber two spotted spider mite.This test 12 medicaments, 2 single doses, 1 blank, repeats for three times, Gong45Ge community.Minizone and surrounding all leave isolation strip, plot area 10m
2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the two spotted spider mite quantity statistics alive of blade before the test and listing mark.Within 5 days after dispenser, investigate and record the two spotted spider mite number alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group is repeated meter of averaging, and calculate Revision insect recluced rate and preventive effect, it the results are shown in Table 7.
Table 7
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| KY-01 | 133 | 42 | 68.42 | 74.47 |
| KY-02 | 125 | 33 | 73.60 | 78.66 |
| KY-03 | 155 | 37 | 76.13 | 80.70 |
| KY-04 | 146 | 37 | 74.66 | 79.51 |
| KY-05 | 178 | 50 | 71.91 | 77.29 |
| KY-06 | 195 | 57 | 70.77 | 76.37 |
| KY-07 | 170 | 75 | 55.88 | 64.33 |
| KY-08 | 132 | 50 | 62.12 | 69.37 |
| KY-09 | 157 | 56 | 64.33 | 71.16 |
| KY-10 | 184 | 66 | 64.13 | 71.00 |
| KY-11 | 118 | 48 | 59.32 | 67.11 |
| KY-12 | 141 | 58 | 58.87 | 66.74 |
| D1 | 186 | 166 | 10.75 | 27.84 |
| D2 | 131 | 123 | 6.11 | 24.09 |
| CK-02 | 114 | 141 | -23.68 | —— |
As can be seen from Table 7, product of the present invention has good preventive effect to two spotted spider mite, and better, visible than the control efficiency of single shrubby sophora extract and Artabotrys odoratissimus extract, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.In addition, have no during dispenser and have poisoning to show to crop.
Test example 3 the present invention contains the efficiency test of the composition pesticide wild cabbage prodenia litura of kuh-seng and Artabotrys odoratissimus extract
Product K Y-01 of the present invention ~ KY-12 is used for preventing and treating wild cabbage prodenia litura.This test 12 medicaments, 2 single doses, 1 blank, repeats for three times, Gong45Ge community.Minizone and surrounding all leave isolation strip, plot area 12m
2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the prodenia litura quantity statistics alive of blade before the test and listing mark.Within 5 days after dispenser, investigate and record the prodenia litura number alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group is repeated meter of averaging, and calculate Revision insect recluced rate and preventive effect, its result is as shown in table 8.
Table 8
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| KY-01 | 245 | 63 | 74.29 | 77.85 |
| KY-02 | 231 | 46 | 80.09 | 82.85 |
| KY-03 | 245 | 42 | 82.86 | 85.23 |
| KY-04 | 235 | 46 | 80.43 | 83.14 |
| KY-05 | 275 | 59 | 78.55 | 81.52 |
| KY-06 | 290 | 65 | 77.59 | 80.69 |
| KY-07 | 201 | 75 | 62.69 | 67.86 |
| KY-08 | 228 | 67 | 70.61 | 74.69 |
| KY-09 | 240 | 66 | 72.50 | 76.31 |
| KY-10 | 231 | 67 | 71.00 | 75.02 |
| KY-11 | 262 | 82 | 68.70 | 73.04 |
| KY-12 | 274 | 94 | 65.69 | 70.45 |
| D1 | 237 | 180 | 24.05 | 34.58 |
| D2 | 216 | 173 | 19.91 | 31.02 |
| CK-02 | 236 | 274 | -16.10 | —— |
As can be seen from Table 8, product of the present invention has good preventive effect to prodenia litura, and better, visible than the control efficiency of single shrubby sophora extract and Artabotrys odoratissimus extract, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.In addition, have no during dispenser and have poisoning to show to crop.
Test example 4 the present invention contains the efficiency test of the composition pesticide wild cabbage diamond-back moth of kuh-seng and Artabotrys odoratissimus extract
Product K Y-01 of the present invention ~ KY-12 is used for preventing and treating wild cabbage diamond-back moth.This test 12 medicaments, 2 single doses, 1 blank, repeats for three times, Gong45Ge community.Minizone and surrounding all leave isolation strip, plot area 10m
2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the diamond-back moth quantity statistics alive of blade before the test and listing mark.Within 5 days after dispenser, investigate and record the diamond-back moth number alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group is repeated meter of averaging, and calculate Revision insect recluced rate and preventive effect, its result is as shown in table 9.
Table 9
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| KY-01 | 233 | 45 | 80.69 | 83.12 |
| KY-02 | 235 | 32 | 86.38 | 88.10 |
| KY-03 | 252 | 29 | 88.49 | 89.94 |
| KY-04 | 264 | 35 | 86.74 | 88.41 |
| KY-05 | 278 | 40 | 85.61 | 87.42 |
| KY-06 | 245 | 39 | 84.08 | 86.09 |
| KY-07 | 234 | 62 | 73.50 | 76.84 |
| KY-08 | 215 | 48 | 77.67 | 80.49 |
| KY-09 | 221 | 47 | 78.73 | 81.41 |
| KY-10 | 254 | 56 | 77.95 | 80.73 |
| KY-11 | 267 | 67 | 74.91 | 78.07 |
| KY-12 | 285 | 72 | 74.74 | 77.92 |
Continued 9
| D1 | 290 | 136 | 53.10 | 59.01 |
| D2 | 253 | 159 | 37.15 | 45.07 |
| CK-02 | 215 | 246 | -14.42 | —— |
As can be seen from Table 9, product of the present invention has good preventive effect to diamond-back moth, and better, visible than the control efficiency of single shrubby sophora extract and Artabotrys odoratissimus extract, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.In addition, have no during dispenser and have poisoning to show to crop.
Test example 5 the present invention contains the efficiency test of the composition pesticide tomato thrips of kuh-seng and Artabotrys odoratissimus extract
Product K Y-01 of the present invention ~ KY-12 is used for preventing and treating tomato thrips.This test 12 medicaments, 2 single doses, 1 blank, repeats for three times, Gong45Ge community.Minizone and surrounding all leave isolation strip, plot area 10m
2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the thrips quantity statistics alive of blade before the test and listing mark.Within 5 days after dispenser, investigate and record the thrips number alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group is repeated meter of averaging, and calculate Revision insect recluced rate and preventive effect, its result is as shown in table 10.
Table 10
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| KY-01 | 143 | 39 | 72.73 | 77.10 |
| KY-02 | 156 | 33 | 78.85 | 82.24 |
| KY-03 | 124 | 24 | 80.65 | 83.75 |
| KY-04 | 127 | 27 | 78.74 | 82.15 |
| KY-05 | 174 | 41 | 76.44 | 80.21 |
Continued 10
| KY-06 | 180 | 44 | 75.56 | 79.47 |
| KY-07 | 196 | 86 | 56.12 | 63.15 |
| KY-08 | 152 | 51 | 66.45 | 71.82 |
| KY-09 | 150 | 48 | 68.00 | 73.13 |
| KY-10 | 141 | 47 | 66.67 | 72.01 |
| KY-11 | 136 | 51 | 62.50 | 68.51 |
| KY-12 | 159 | 64 | 59.75 | 66.20 |
| D1 | 123 | 101 | 17.89 | 31.05 |
| D2 | 164 | 148 | 9.76 | 24.22 |
| CK-02 | 131 | 156 | -19.08 | —— |
As can be seen from Table 10, product of the present invention has good preventive effect to thrips, and better, visible than the control efficiency of single shrubby sophora extract and Artabotrys odoratissimus extract, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.In addition, have no during dispenser and have poisoning to show to crop.
Test example 6 the present invention contains the efficiency test of the composition pesticide oranges and tangerines cottonycushion scale of kuh-seng and Artabotrys odoratissimus extract
Product K Y-01 of the present invention ~ KY-12 is used for preventing and treating oranges and tangerines cottonycushion scale.This test 12 medicaments, 2 single doses, 1 blank, repeats for three times, Gong45Ge community.Minizone and surrounding all leave isolation strip, plot area 20m
2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the cottonycushion scale quantity statistics alive of blade before the test and listing mark.Within 5 days after dispenser, investigate and record the cottonycushion scale number alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group is repeated meter of averaging, and calculate Revision insect recluced rate and preventive effect, its result is as shown in table 11.
Table 11
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| KY-01 | 147 | 48 | 67.35 | 71.61 |
| KY-02 | 132 | 34 | 74.24 | 77.61 |
| KY-03 | 152 | 36 | 76.32 | 79.41 |
| KY-04 | 166 | 42 | 74.70 | 78.00 |
| KY-05 | 115 | 32 | 72.17 | 75.81 |
| KY-06 | 135 | 40 | 70.37 | 74.24 |
| KY-07 | 167 | 75 | 55.09 | 60.96 |
| KY-08 | 174 | 66 | 62.07 | 67.02 |
| KY-09 | 153 | 55 | 64.05 | 68.75 |
| KY-10 | 176 | 66 | 62.50 | 67.40 |
| KY-11 | 153 | 63 | 58.82 | 64.20 |
| KY-12 | 185 | 77 | 58.38 | 63.82 |
| D1 | 136 | 118 | 13.24 | 24.57 |
| D2 | 121 | 110 | 9.09 | 20.97 |
| CK-02 | 173 | 199 | -15.03 | —— |
As can be seen from Table 11, product of the present invention has good preventive effect to oranges and tangerines cottonycushion scale, and better, visible than the control efficiency of single shrubby sophora extract and Artabotrys odoratissimus extract, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.In addition, have no during dispenser and have poisoning to show to crop.
Claims (17)
1. contain the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, it is characterized in that, its active component is extracted by the crude drug of following weight portion and forms: kuh-seng 1 part, Artabotrys odoratissimus 1 ~ 10 part, and the extracting method of active component is: take kuh-seng particle and Artabotrys odoratissimus particle, mixing, adds solvent by solid-liquid ratio 1:3 ~ 12g/ml, regulate pH to 5-7, in 60 ~ 100 DEG C of refluxing extraction, filter, obtain extract, wherein, described solvent is methyl alcohol, ethanol, ethyl acetate, acetone, chloroform or water.
2. the composition pesticide containing kuh-seng and Artabotrys odoratissimus extract according to claim 1, is characterized in that, its active component is extracted by the crude drug of following weight portion and forms: kuh-seng 1 part, Artabotrys odoratissimus 3 ~ 8 parts.
3. the composition pesticide containing kuh-seng and Artabotrys odoratissimus extract according to claim 2, is characterized in that, its active component is extracted by the crude drug of following weight portion and forms: kuh-seng 1 part, Artabotrys odoratissimus 3 ~ 6 parts.
4. the composition pesticide containing kuh-seng and Artabotrys odoratissimus extract according to claim 3, is characterized in that, its active component is extracted by the crude drug of following weight portion and forms: kuh-seng 1 part, Artabotrys odoratissimus 5 parts.
5. the composition pesticide containing kuh-seng and Artabotrys odoratissimus extract according to any one of Claims 1 to 4, is characterized in that: the described composition pesticide containing kuh-seng and Artabotrys odoratissimus extract is microemulsion, aqua or soluble concentrate.
6. the composition pesticide containing kuh-seng and Artabotrys odoratissimus extract according to any one of Claims 1 to 4, it is characterized in that: described containing in the composition pesticide of kuh-seng and Artabotrys odoratissimus extract also comprises auxiliary agent, and described auxiliary agent is at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
7. the composition pesticide containing kuh-seng and Artabotrys odoratissimus extract according to claim 5, it is characterized in that: described containing in the composition pesticide of kuh-seng and Artabotrys odoratissimus extract also comprises auxiliary agent, and described auxiliary agent is at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
8. the composition pesticide containing kuh-seng and Artabotrys odoratissimus extract according to claim 5, it is characterized in that: described microemulsion is made up of the component of following weight portion: kuh-seng and Artabotrys odoratissimus extract, 15 parts of emulsifier op-10,3 parts of emulsifier, 500,5 parts of dimethyl sulfoxide (DMSO)s, 10 parts of ethanol, 5 parts of ethylene glycol, 22 parts of water, wherein, kuh-seng and Artabotrys odoratissimus extract are that the crude drug extraction of 40 parts forms.
9. the composition pesticide containing kuh-seng and Artabotrys odoratissimus extract according to claim 7, it is characterized in that: described microemulsion is made up of the component of following weight portion: kuh-seng and Artabotrys odoratissimus extract, 15 parts of emulsifier op-10,3 parts of emulsifier, 500,5 parts of dimethyl sulfoxide (DMSO)s, 10 parts of ethanol, 5 parts of ethylene glycol, 22 parts of water, wherein, kuh-seng and Artabotrys odoratissimus extract are that the crude drug extraction of 40 parts forms.
10. the composition pesticide containing kuh-seng and Artabotrys odoratissimus extract according to claim 8, is characterized in that: kuh-seng is prepared from by the following method with Artabotrys odoratissimus extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
11. composition pesticides containing kuh-seng and Artabotrys odoratissimus extract according to claim 9, is characterized in that: kuh-seng is prepared from by the following method with Artabotrys odoratissimus extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
The method containing the composition pesticide of kuh-seng and Artabotrys odoratissimus extract described in 12. any one of preparation claim 1 ~ 11, is characterized in that being made up of following steps:
A, extraction: take kuh-seng particle and Artabotrys odoratissimus particle, mixing, adds solvent by solid-liquid ratio 1:3 ~ 12g/ml, regulate pH to 5-7, in 60 ~ 100 DEG C of refluxing extraction, filter, obtain extract, wherein, described solvent is methyl alcohol, ethanol, ethyl acetate, acetone, chloroform or water;
B, preparation: extract is made microemulsion, soluble concentrate or aqua.
13. preparations according to claim 12 contain the method for the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, and it is characterized in that: in described a step, kuh-seng particle and Artabotrys odoratissimus particle cross 60 ~ 100 mesh sieves; Solid-liquid ratio is 1:4 ~ 10g/ml; PH value is 6.0; Solvent is water; Reflow's cycle is twice, and merging filtrate, obtains extract.
14. preparations according to claim 13 contain the method for the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, and it is characterized in that: in described a step, kuh-seng particle and Artabotrys odoratissimus particle cross 80 mesh sieves; Solid-liquid ratio is 1:6g/ml.
The composition pesticide containing kuh-seng and Artabotrys odoratissimus extract described in 15. any one of claim 1 ~ 11 is preventing and treating the application in crop pest.
16. application of composition pesticide in control crop pest containing kuh-seng and Artabotrys odoratissimus extract according to claim 15, is characterized in that: described insect pest is Homoptera, Acarina, thrips, lepidoptera pest; During application, be diluted with water to the activity component concentration of crude drug weighing scale as 10 ~ 100ppm.
17. application of composition pesticide in control crop pest containing kuh-seng and Artabotrys odoratissimus extract according to claim 16, is characterized in that: be diluted with water to the activity component concentration of crude drug weighing scale as 20 ~ 50ppm.
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