CN103689002B - Pesticide composition containing lantana camara and azolla imbricata extracts, preparation method and application - Google Patents
Pesticide composition containing lantana camara and azolla imbricata extracts, preparation method and application Download PDFInfo
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- CN103689002B CN103689002B CN201310745702.8A CN201310745702A CN103689002B CN 103689002 B CN103689002 B CN 103689002B CN 201310745702 A CN201310745702 A CN 201310745702A CN 103689002 B CN103689002 B CN 103689002B
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a pesticide composition containing lantana camara and azolla imbricata extracts, a preparation method and application thereof, belonging to the field of pesticides. The invention aims to solve the technical problem of providing a pesticide composition containing lantana camara and azolla imbricata extracts, a preparation method and application thereof. The invention relates to a pesticide composition containing lantana camara and azolla imbricata extracts, which is prepared by extracting the following raw medicinal materials in parts by weight: 1 part of lantana camara and 0.2-4 parts of azolla imbricata; the active ingredients can be prepared by mixing the raw medicinal materials of lantana camara and azolla imbricata and then extracting together; or extracting lantana camara separately to obtain lantana camara extract, extracting azolla imbricata separately to obtain azolla imbricata extract, and mixing the two extracts. The pesticide composition containing the lantana camara and the azolla imbricata extracts has obvious control effect on lepidoptera and homoptera pests, and has positive significance for agricultural production.
Description
Technical field
The present invention relates to the composition pesticide containing lantana and Azolla imbricata extract and preparation method and application, belong to pesticide field.
Background technology
In recent years, in agriculture and forestry field, due to single drug and not Scientific Usage of Drugs, plant pest has produced pesticide resistance in various degree, and agriculture and forestry produce still with chemical pesticide for mainly to prevent and treat kind, cause the destruction increasingly of environmental pollution and ecotope.Along with the raising of people's living standard and the enhancing of health perception, the manufacture and exploit of biopesticide just progressively receives the concern of agricultural chemicals R&D worker, becomes the focus of agriculture and forestry sustainable development, simultaneously, also be environmental protect, the effective way of minimizing or delaying drug resistance and raising drug effect.Wherein, botanical pesticide has become one of focus of biopesticide research.
Lantana, is again Lantana camara, stinkweed, grass as one wishes.Belonging to Verenaceae Lantana, is perennial upright or half rattan shape shrub.Lantana originates in american torrid zone area, and all there is distribution the provinces and regions such as China Guangdong, Guangxi, Yunnan, Fujian, Taiwan.Except often cultivating for except viewing and admiring in flower garden, root, leaf, flower also can be used as medicine, and have the effect of heat-clearing, removing toxic substances, hemostasis.
Azolla imbricata, the plant of Azolla imbricata section azolla, grows the small-sized floating plant in paddy field or pond.In green during children, growth rapidly, the water surface of being everlasting grows up to a slice.In time autumn and winter, containing a lot of anthocyan in its leaf, colony presents a slice redness, so be called Azolla imbricata.Azolla imbricata can all herbal medicine, promoting eruption of inducing sweat, expelling wind and removing dampness.Can be used for measles without adequate eruption, rheumatic arthritis, nettle rash, in the treatment of the diseases such as pruitus.
In test, the present inventor finds that lantana and the common extract of Azolla imbricata and extract from Lantana camara and the mixing of Azolla imbricata extract have good synergistic function, can insecticide be prepared into, have good preventive effect to cabbage caterpillar, diamond-back moth, aphid, trialeurodes vaporariorum etc.
Summary of the invention
The technical problem to be solved in the present invention is to provide composition pesticide containing lantana and Azolla imbricata extract and preparation method and application.
The present invention contains the composition pesticide of lantana and Azolla imbricata extract, and its active component is extracted by the crude drug of following weight portion and forms: lantana 1 part, Azolla imbricata 0.2 ~ 4 part.Wherein, active component is preferably extracted by the crude drug of following weight portion and forms: lantana 1 part, Azolla imbricata 0.5 ~ 2 part; Be preferably again and formed by the crude drug extraction of following weight portion: lantana 1 part, Azolla imbricata 0.5 ~ 1.5 part; Be more preferably and formed by the crude drug extraction of following weight portion: lantana 1 part, Azolla imbricata 1 part.
Wherein, the lantana that the present invention adopts and Azolla imbricata are Chinese herbal medicine, contain the activity of the composition pesticide of lantana and Azolla imbricata extract in order to improve the present invention, the Leaves of Lantana Camara that lantana is preferably dry, the Azolla imbricata herb that Azolla imbricata is preferably dry.
The present invention contains the composition pesticide of lantana and Azolla imbricata extract, and after its active component can be mixed by crude drug lantana and Azolla imbricata, common extraction forms; Also first can be extracted separately by lantana and obtain extract from Lantana camara, Azolla imbricata extracts separately and obtains Azolla imbricata extract, then is mixed by two kinds of extracts.In order to improve the insecticidal properties of composition pesticide of the present invention, after its active component is preferably and is mixed by lantana and Azolla imbricata, common extraction forms.
Further, in order to improve the stability of the composition pesticide containing lantana and Azolla imbricata extract, the present invention contains in the composition pesticide of lantana and Azolla imbricata extract and also comprises auxiliary agent, described auxiliary agent can adopt the usual auxiliaries in agricultural chemicals, as at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
Wherein, emulsifier can adopt at least one in non-ionic surface active agent and anion surfactant, described non-ionic surface active agent can be dibenzyl xenyl APEO, styryl phenyl APEO, styryl phenyl APEO, phenethyl phenyl polyoxyethylene one polyethenoxy ether, OPEO, alkylphenol polyoxyethylene sulfosuccinate, aliphatic acid polyethenoxy ether, fatty alcohol-polyoxyethylene ether, aliphatic amine polyoxyethylene ether, castor oil ethylene oxide adduct and derivative thereof, at least one in polyol fatty acid ester and ethylene oxide adduct etc. thereof, described anion surfactant is at least one in calcium dodecyl benzene sulfonate, alkylphenol polyoxyethylene phosphate, phenethyl phenol polyethenoxy ether phosphate etc., as emulsifier 500,601p, 602p etc., in addition, commercially available emulsifier production can also be used.
Wherein, solvent can adopt at least one in water, ethanol, isopropyl alcohol, n-butanol, ethyl acetate, butyl acetate, methyl-sulfoxide, turpentine oil, solvent naphtha, 1-METHYLPYRROLIDONE, dimethyl formamide etc.
Wherein, antifreeze can be glycerine, propane diols, ethylene glycol, sodium chloride, calcium chloride, sorbierite etc.
Wherein, defoamer is silicone defoaming agent etc.
Wherein, dispersant is at least one in lignosulfonates, PAA and propionamide copolymer, alkylnaphthalene sulfonate, polycarboxylic acid salt's condensation product, methyl sodiosul foaliphatate etc.
Wherein, wetting agent can be alkyl sulfate, alkylbenzenesulfonate, alkylnaphthalene sulfonate, aliphatic alcohol polyethenoxy base ether, alkylphenol-polyethenoxy base ether, sorbitan fatty acid ester etc.
Wherein, stabilizing agent can be 2-expoxy propane, butyl glycidyl ether, isopropyl alcohol, n-butanol, polyethylene glycol etc.
The present invention can be prepared into any one formulation of applicable agricultural use containing the composition pesticide of lantana and Azolla imbricata extract by the method for this area routine, for the ease of storing and transport, can make microemulsion, aqua or soluble concentrate.
Further, the soluble concentrate be preferably made up of the component of following weight portion: lantana and Azolla imbricata extract, 10 parts of emulsifier, 602,40 parts of ethanol, wherein, lantana and Azolla imbricata extract are that the crude drug extraction of 50 parts forms.
For the ease of storing and transport, extract can be concentrated, obtain concentrate, and then be prepared into various formulation, cycles of concentration can according to needs of production from Row sum-equal matrix, preferably, lantana is prepared from by the following method with Azolla imbricata extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
Second technical problem that the present invention will solve is to provide the method that preparation contains the composition pesticide of lantana and Azolla imbricata extract.
The preparation method that the present invention contains the composition pesticide of lantana and Azolla imbricata extract is made up of following steps:
A, extraction: take lantana particle and Azolla imbricata particle, mixing, adds solvent by solid-liquid ratio 1:3 ~ 12g/ml, and in 60 ~ 95 DEG C of refluxing extraction, filter, obtain extract, wherein, described solvent is organic solvent or water;
B, preparation: extract is made microemulsion, soluble concentrate or aqua.
Wherein, lantana particle and Azolla imbricata particle can adopt commercially available particle, and conventional method pulverizing Leaves of Lantana Camara and Azolla imbricata herb also can be adopted to obtain.In order to improve the effect of extraction, simultaneously cost-saving, lantana particle and Azolla imbricata particle cross 60 ~ 100 mesh sieves, preferred mistake 80 mesh sieve.
Further, in order to cost-saving, simultaneously also in order to improve extraction efficiency, the solid-liquid ratio in a step is preferably 1:4 ~ 10g/ml, is more preferably 1:6g/ml.Namely the lantana of lg and Azolla imbricata particle can add 3 ~ 12ml solvent extraction, preferably add 4 ~ 10ml, more preferably add 6ml.
Conventional organic solvent all can be used for the present invention, and in order to cost-saving, improve dissolution rate, ensure extraction efficiency, organic solvent is preferably methyl alcohol, ethanol, ethyl acetate, acetone, chloroform, is more preferably absolute ethyl alcohol simultaneously.
In order to shorten preparation time, accelerate extraction rate, can stir when adding hot reflux, mixing speed is preferably 80 ~ 100 revs/min.
Further, reflux temperature is preferably 75 ~ 85 DEG C, is more preferably 82 DEG C; Reflow's cycle is twice, and each return time is 2.5 hours, and merging filtrate, obtains extract.
The application of the composition pesticide that the present invention also provides the present invention to contain lantana and Azolla imbricata extract in control crop pest.The present invention contains the composition pesticide of lantana and Azolla imbricata extract, to lepidoptera pest as cabbage caterpillar, diamond-back moth, prodenia litura, beet snout moth's larva, tea tree tea geometrid; Homoptera pest such as aphid, scale insect, trialeurodes vaporariorum etc. have good preventive effect.
In the agricultural production of reality, in order to improve composition pesticide containing lantana and Azolla imbricata extract to the control efficiency of insect pest, the described composition pesticide containing lantana and Azolla imbricata extract is preferably applied to crop pest initial stage of origination; The present invention can use by common method containing the composition pesticide of lantana and Azolla imbricata extract, sprays on crop blade face.Preferably, when using, the present invention is diluted with water to the activity component concentration of crude drug weighing scale as 10 ~ 100ppm containing the composition pesticide of lantana and Azolla imbricata extract, namely the composition pesticide that 1g crude drug is obtained after extracting, when using, be diluted with water to 10kg, then its activity component concentration equals 100ppm.Preferably being diluted to activity component concentration is further that 20 ~ 50ppm sprays in plant blade face, and so that water membrane to appear in blade face, not lower of liquid is advisable; Secondary dispenser is carried out, the preferred continuous administration of a season crop 2 ~ 3 times after being preferable over 3 ~ 5 days.
The present invention is mixed by extract from Lantana camara and Azolla imbricata extract containing the composition pesticide of lantana and Azolla imbricata extract, or jointly extracted by lantana and Azolla imbricata and form, its preventive effect is far away higher than the extract of single component, particularly extract common after lantana and Azolla imbricata mixing, owing to having solubilising, hydrotropy effect between lantana and Azolla imbricata, the composition pesticide insecticidal effect made after common extraction is more remarkable.
Compared with prior art, the present invention has the following advantages containing the composition pesticide of lantana and Azolla imbricata extract:
1, the present invention has synergistic function containing the composition pesticide of lantana and Azolla imbricata extract, obvious to the control efficiency of Lepidoptera, homoptera pest.
2, lantana of the present invention and Azolla imbricata mix after jointly extracting or extracting respectively, and the composition pesticide made can improve crop immune system ability while quick-acting tagging, and can significantly improve its vigor.
3, the present invention take Chinese herbal medicine as raw material, thus there is not Pesticide Residue, and insect is difficult to produce resistance; Be used alone compared with chemical source agricultural chemicals, environmentally friendly.
4, when the present invention uses containing the composition pesticide of lantana and Azolla imbricata extract, valid density is low, with low cost.
Embodiment
The present invention contains the composition pesticide of lantana and Azolla imbricata extract, and its active component is extracted by the crude drug of following weight portion and forms: lantana 1 part, Azolla imbricata 0.2 ~ 4 part.Wherein, active component is preferably extracted by the crude drug of following weight portion and forms: lantana 1 part, Azolla imbricata 0.5 ~ 2 part; Be preferably again and formed by the crude drug extraction of following weight portion: lantana 1 part, Azolla imbricata 0.5 ~ 1.5 part; Be more preferably and formed by the crude drug extraction of following weight portion: lantana 1 part, Azolla imbricata 1 part.
Wherein, the lantana that the present invention adopts and Azolla imbricata are Chinese herbal medicine, contain the activity of the composition pesticide of lantana and Azolla imbricata extract in order to improve the present invention, the Leaves of Lantana Camara that lantana is preferably dry, the Azolla imbricata herb that Azolla imbricata is preferably dry.
The present invention contains the composition pesticide of lantana and Azolla imbricata extract, and after its active component can be mixed by crude drug lantana and Azolla imbricata, common extraction forms; Also first can be extracted separately by lantana and obtain extract from Lantana camara, Azolla imbricata extracts separately and obtains Azolla imbricata extract, then is mixed by two kinds of extracts.In order to improve the insecticidal properties of composition pesticide of the present invention, after its active component is preferably and is mixed by lantana and Azolla imbricata, common extraction forms.
Further, in order to improve the stability of the composition pesticide containing lantana and Azolla imbricata extract, the present invention contains in the composition pesticide of lantana and Azolla imbricata extract and also comprises auxiliary agent, described auxiliary agent can adopt the usual auxiliaries in agricultural chemicals, as at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
Wherein, emulsifier can adopt at least one in non-ionic surface active agent and anion surfactant, described non-ionic surface active agent can be dibenzyl xenyl APEO, styryl phenyl APEO, styryl phenyl APEO, phenethyl phenyl polyoxyethylene one polyethenoxy ether, OPEO, alkylphenol polyoxyethylene sulfosuccinate, aliphatic acid polyethenoxy ether, fatty alcohol-polyoxyethylene ether, aliphatic amine polyoxyethylene ether, castor oil ethylene oxide adduct and derivative thereof, at least one in polyol fatty acid ester and ethylene oxide adduct etc. thereof, described anion surfactant is at least one in calcium dodecyl benzene sulfonate, alkylphenol polyoxyethylene phosphate, phenethyl phenol polyethenoxy ether phosphate etc., as emulsifier 500,601p, 602p etc., in addition, commercially available emulsifier production can also be used.
Wherein, solvent can adopt at least one in water, ethanol, isopropyl alcohol, n-butanol, ethyl acetate, butyl acetate, methyl-sulfoxide, turpentine oil, solvent naphtha, 1-METHYLPYRROLIDONE, dimethyl formamide etc.
Wherein, antifreeze can be glycerine, propane diols, ethylene glycol, sodium chloride, calcium chloride, sorbierite etc.
Wherein, defoamer is silicone defoaming agent etc.
Wherein, dispersant is at least one in lignosulfonates, PAA and propionamide copolymer, alkylnaphthalene sulfonate, polycarboxylic acid salt's condensation product, methyl sodiosul foaliphatate etc.
Wherein, wetting agent can be alkyl sulfate, alkylbenzenesulfonate, alkylnaphthalene sulfonate, aliphatic alcohol polyethenoxy base ether, alkylphenol-polyethenoxy base ether, sorbitan fatty acid ester etc.
Wherein, stabilizing agent can be 2-expoxy propane, butyl glycidyl ether, isopropyl alcohol, n-butanol, polyethylene glycol etc.
The present invention can be prepared into any one formulation of applicable agricultural use containing the composition pesticide of lantana and Azolla imbricata extract by the method for this area routine, for the ease of storing and transport, can make microemulsion, aqua or soluble concentrate.
Further, the soluble concentrate be preferably made up of the component of following weight portion: lantana and Azolla imbricata extract, 10 parts of emulsifier, 602,40 parts of ethanol, wherein, lantana and Azolla imbricata extract are that the crude drug extraction of 50 parts forms.
For the ease of storing and transport, extract can be concentrated, obtain concentrate, and then be prepared into various formulation, cycles of concentration can according to needs of production from Row sum-equal matrix, preferably, lantana is prepared from by the following method with Azolla imbricata extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
The preparation method that the present invention contains the composition pesticide of lantana and Azolla imbricata extract is made up of following steps:
A, extraction: take lantana particle and Azolla imbricata particle, mixing, adds solvent by solid-liquid ratio 1:3 ~ 12g/ml, and in 60 ~ 95 DEG C of refluxing extraction, filter, obtain extract, wherein, described solvent is organic solvent or water;
B, preparation: extract is made microemulsion, soluble concentrate or aqua.
Wherein, lantana particle and Azolla imbricata particle can adopt commercially available particle, and conventional method pulverizing Leaves of Lantana Camara and Azolla imbricata herb also can be adopted to obtain.In order to improve the effect of extraction, simultaneously cost-saving, lantana particle and Azolla imbricata particle cross 60 ~ 100 mesh sieves, preferred mistake 80 mesh sieve.
Further, in order to cost-saving, simultaneously also in order to improve extraction efficiency, the solid-liquid ratio in a step is preferably 1:4 ~ 10g/ml, is more preferably 1:6g/ml.Namely the lantana of lg and Azolla imbricata particle can add 3 ~ 12ml solvent extraction, preferably add 4 ~ 10ml, more preferably add 6ml.
Conventional organic solvent all can be used for the present invention, and in order to cost-saving, improve dissolution rate, ensure extraction efficiency, organic solvent is preferably methyl alcohol, ethanol, ethyl acetate, acetone, chloroform, is more preferably absolute ethyl alcohol simultaneously.
In order to shorten preparation time, accelerate extraction rate, can stir when adding hot reflux, mixing speed is preferably 80 ~ 100 revs/min.
Further, reflux temperature is preferably 75 ~ 85 DEG C, is more preferably 82 DEG C; Reflow's cycle is twice, and each return time is 2.5 hours, and merging filtrate, obtains extract.
The present invention contains the composition pesticide of lantana and Azolla imbricata extract, to lepidoptera pest as cabbage caterpillar, diamond-back moth, prodenia litura, beet snout moth's larva, tea tree tea geometrid; Homoptera pest such as aphid, scale insect, trialeurodes vaporariorum etc. have good preventive effect.
In the agricultural production of reality, in order to improve composition pesticide containing lantana and Azolla imbricata extract to the control efficiency of insect pest, the described composition pesticide containing lantana and Azolla imbricata extract is preferably applied to crop pest initial stage of origination; The present invention can use by common method containing the composition pesticide of lantana and Azolla imbricata extract, sprays on crop blade face.Preferably, when using, the present invention is diluted with water to the activity component concentration of crude drug weighing scale as 10 ~ 100ppm containing the composition pesticide of lantana and Azolla imbricata extract, namely the composition pesticide that 1g crude drug is obtained after extracting, when using, be diluted with water to 10kg, then its activity component concentration equals 100ppm.Preferably being diluted to activity component concentration is further that 20 ~ 50ppm sprays in plant blade face, and so that water membrane to appear in blade face, not lower of liquid is advisable; Secondary dispenser is carried out, the preferred continuous administration of a season crop 2 ~ 3 times after being preferable over 3 ~ 5 days.
The present invention is mixed by extract from Lantana camara and Azolla imbricata extract containing the composition pesticide of lantana and Azolla imbricata extract, or jointly extracted by lantana and Azolla imbricata and form, its preventive effect is far away higher than the extract of single component, particularly extract common after lantana and Azolla imbricata mixing, owing to having solubilising, hydrotropy effect between lantana and Azolla imbricata, the composition pesticide insecticidal effect made after common extraction is more remarkable.
Below in conjunction with embodiment, the specific embodiment of the present invention is further described, does not therefore limit the present invention among described scope of embodiments.
Embodiment 1 contains the preparation of the composition pesticide of lantana and Azolla imbricata extract
A, extraction: take dry Leaves of Lantana Camara and Azolla imbricata herb, mixing, pulverizes, and crosses 80 mesh sieves, adds extraction solution, refluxing extraction twice, filters, merging filtrate, concentrated, makes extract weight equal the weight of crude drug, obtains extract mm-01 ~ mm-06; Wherein, each parameter is in table 1;
B, preparation: in extract mm-01 ~ mm-06, add auxiliary agent in table 2, stir, must contain the composition pesticide MM-01 ~ MM-06 of lantana and extract from Lantana camara.
The preparation technology parameter of table 1 lantana and Azolla imbricata extract
Wherein, Leaves of Lantana Camara and Azolla imbricata herb are all purchased from Anguo City Zhong Tian prepared slices of Chinese crude drugs Co., Ltd.
Table 2 contains the formula of the composition pesticide of lantana and Azolla imbricata extract
Embodiment 2 contains the preparation of the composition pesticide of lantana and Azolla imbricata extract
A, extraction: take dry Leaves of Lantana Camara, pulverize, and crosses 80 mesh sieves, add extraction solution, refluxing extraction twice, filters, merging filtrate, and concentrated, make extract weight equal the weight of crude drug, obtain extract d1-01 ~ d1-03, wherein, each parameter is in table 3;
Take dry Azolla imbricata herb, pulverize, cross 80 mesh sieves, add extraction solution, refluxing extraction twice, filter, merging filtrate, concentrated, make extract weight equal the weight of crude drug, obtain extract d2-01 ~ d2-03, wherein, each parameter is in table 4;
B, preparation: take lantana extract, Azolla imbricata extract and auxiliary agent, stirring and evenly mixing, obtain the composition pesticide MM-07 ~ MM-12 containing lantana and Azolla imbricata extract, wherein, each parameter is in table 5.
Table 3
Table 4
Table 5
Test example 1 the present invention contains the efficiency test of the composition pesticide wild cabbage diamond-back moth of lantana and Azolla imbricata extract
For clearly putting into field efficacy situation containing the composition pesticide of lantana and Azolla imbricata extract, inventor has carried out field control effectiveness test to this medicament.
1, test material
1.1 for studying thing and object: wild cabbage diamond-back moth
1.2 reagent agent
Product in embodiment: MM-01 ~ MM-12;
Lantana single dose (D1), Azolla imbricata single dose (D2):
Chemical reagent contrast (CK-01): gamma cyhalothrin, purchased from Chengdu Kelilong Biochemical Co., Ltd.;
Clear water contrast (CK-02).
Wherein, the preparation method of lantana single dose is: the d1-03 getting 50g, adds 10g emulsifier 602,40g ethanol, stirring and evenly mixing, obtained lantana single dose.
The preparation method of Azolla imbricata single dose is: the d2-03 getting 50g, adds 10g emulsifier 602,40g ethanol, stirring and evenly mixing, obtained Azolla imbricata single dose.
2, experimental scheme and method
This test 14 medicaments, 1 contrast medicament, 1 blank, repeats for three times, Gong48Ge community.Minizone and surrounding all leave isolation strip, plot area 12m
2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the diamond-back moth quantity statistics alive of blade before the test and listing mark.Within 5 days after dispenser, investigate and record the diamond-back moth number alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
3, result of the test
Within 5 days after dispenser, investigate and record the borer population alive on each blade, calculate Revision insect recluced rate and preventive effect, it the results are shown in Table 6.
Table 6
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| MM-01 | 215 | 47 | 78.14 | 82.35 |
| MM-02 | 255 | 34 | 86.67 | 89.23 |
| MM-03 | 264 | 32 | 87.88 | 90.21 |
| MM-04 | 243 | 32 | 86.83 | 89.37 |
| MM-05 | 232 | 36 | 84.48 | 87.47 |
| MM-06 | 226 | 41 | 81.86 | 85.35 |
| MM-07 | 267 | 83 | 68.91 | 74.90 |
| MM-08 | 286 | 73 | 74.48 | 79.39 |
| MM-09 | 295 | 72 | 75.59 | 80.29 |
Continued 6
| MM-10 | 257 | 65 | 74.71 | 79.58 |
| MM-11 | 241 | 65 | 73.03 | 78.22 |
| MM-12 | 235 | 67 | 71.49 | 76.98 |
| D1 | 257 | 212 | 17.51 | 33.39 |
| D2 | 259 | 220 | 15.06 | 31.41 |
| CK-01 | 233 | 23 | 90.13 | 92.03 |
| CK-02 | 214 | 265 | -23.83 | —— |
As can be seen from Table 6, product of the present invention has good preventive effect to diamond-back moth, and better, visible than the control efficiency of single extract from Lantana camara and Azolla imbricata extract, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.
Test example 2 the present invention contains the efficiency test of the composition pesticide tea tree tea geometrid of lantana and Azolla imbricata extract
Product MM-01 ~ MM-12 of the present invention is used for preventing and treating tea tree tea geometrid.This test 12 medicaments, 2 single doses, 1 blank, repeats for three times, Gong45Ge community.Minizone and surrounding all leave isolation strip, plot area 10m
2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the tea geometrid quantity statistics alive of blade before the test and listing mark.Within 5 days after dispenser, investigate and record the tea geometrid number alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group is repeated meter of averaging, and calculate Revision insect recluced rate and preventive effect, it the results are shown in Table 7.
Table 7
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| MM-01 | 147 | 41 | 72.11 | 76.53 |
Continued 7
| MM-02 | 135 | 27 | 80.00 | 83.17 |
| MM-03 | 146 | 27 | 81.51 | 84.44 |
| MM-04 | 158 | 31 | 80.38 | 83.49 |
| MM-05 | 118 | 26 | 77.97 | 81.46 |
| MM-06 | 128 | 31 | 75.78 | 79.62 |
| MM-07 | 126 | 46 | 63.49 | 69.28 |
| MM-08 | 168 | 53 | 68.45 | 73.46 |
| MM-09 | 169 | 48 | 71.60 | 76.10 |
| MM-10 | 167 | 51 | 69.46 | 74.31 |
| MM-11 | 159 | 51 | 67.92 | 73.01 |
| MM-12 | 174 | 59 | 66.09 | 71.47 |
| D1 | 186 | 162 | 12.90 | 26.72 |
| D2 | 147 | 131 | 10.88 | 25.02 |
| CK-02 | 122 | 145 | -18.85 | —— |
As can be seen from Table 7, product of the present invention has good preventive effect to tea geometrid, and better, visible than the control efficiency of single extract from Lantana camara and Azolla imbricata extract, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.In addition, have no during dispenser and have poisoning to show to crop.
Test example 3 the present invention contains the efficiency test of the composition pesticide wild cabbage prodenia litura of lantana and Azolla imbricata extract
Product MM-01 ~ MM-12 of the present invention is used for preventing and treating wild cabbage prodenia litura.This test 12 medicaments, 2 single doses, 1 blank, repeats for three times, Gong45Ge community.Minizone and surrounding all leave isolation strip, plot area 20m
2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the prodenia litura quantity statistics alive of blade before the test and listing mark.Within 5 days after dispenser, investigate and record the prodenia litura number alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group is repeated meter of averaging, and calculate Revision insect recluced rate and preventive effect, its result is as shown in table 8.
Table 8
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| MM-01 | 224 | 57 | 74.55 | 78.26 |
| MM-02 | 245 | 49 | 80.00 | 82.91 |
| MM-03 | 253 | 43 | 83.00 | 85.48 |
| MM-04 | 265 | 50 | 81.13 | 83.88 |
| MM-05 | 274 | 60 | 78.10 | 81.29 |
| MM-06 | 216 | 50 | 76.85 | 80.22 |
| MM-07 | 285 | 106 | 62.81 | 68.22 |
| MM-08 | 298 | 91 | 69.46 | 73.91 |
| MM-09 | 289 | 84 | 70.93 | 75.17 |
| MM-10 | 247 | 75 | 69.64 | 74.06 |
| MM-11 | 263 | 86 | 67.30 | 72.06 |
| MM-12 | 285 | 97 | 65.96 | 70.92 |
| D1 | 212 | 185 | 12.74 | 25.45 |
| D2 | 244 | 218 | 10.66 | 23.67 |
| CK-02 | 217 | 254 | -17.05 | —— |
As can be seen from Table 8, product of the present invention has good preventive effect to prodenia litura, and better, visible than the control efficiency of single extract from Lantana camara and Azolla imbricata extract, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.In addition, have no during dispenser and have poisoning to show to crop.
Test example 4 the present invention contains the efficiency test of the composition pesticide pumpkin aphid of lantana and Azolla imbricata extract
Product MM-01 ~ MM-12 of the present invention is used for preventing and treating pumpkin aphid.This test 12 medicaments, 2 single doses, 1 blank, repeats for three times, Gong45Ge community.Minizone and surrounding all leave isolation strip, plot area 10m
2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the Aphed population statistics alive also listing mark of blade before the test.Within 5 days after dispenser, investigate and record the aphid number alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group is repeated meter of averaging, and calculate Revision insect recluced rate and preventive effect, its result is as shown in table 9.
Table 9
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| MM-01 | 422 | 84 | 80.09 | 82.10 |
| MM-02 | 431 | 58 | 86.54 | 87.90 |
| MM-03 | 441 | 54 | 87.76 | 88.99 |
| MM-04 | 453 | 60 | 86.75 | 88.09 |
| MM-05 | 465 | 70 | 84.95 | 86.46 |
| MM-06 | 457 | 76 | 83.37 | 85.04 |
| MM-07 | 485 | 138 | 71.55 | 74.41 |
| MM-08 | 447 | 108 | 75.84 | 78.27 |
| MM-09 | 497 | 111 | 77.67 | 79.91 |
| MM-10 | 406 | 97 | 76.11 | 78.51 |
| MM-11 | 437 | 111 | 74.60 | 77.15 |
| MM-12 | 424 | 112 | 73.58 | 76.24 |
| D1 | 418 | 306 | 26.79 | 34.16 |
| D2 | 426 | 326 | 23.47 | 31.17 |
| CK-02 | 447 | 497 | -11.19 | —— |
As can be seen from Table 9, product of the present invention has good preventive effect to aphid, and better, visible than the control efficiency of single extract from Lantana camara and Azolla imbricata extract, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.In addition, have no during dispenser and have poisoning to show to crop.
Test example 5 the present invention contains the efficiency test of the composition pesticide eggplant trialeurodes vaporariorum of lantana and Azolla imbricata extract
Product MM-01 ~ MM-12 of the present invention is used for preventing and treating eggplant trialeurodes vaporariorum.This test 12 medicaments, 2 single doses, 1 blank, repeats for three times, Gong45Ge community.Minizone and surrounding all leave isolation strip, plot area 10m
2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the trialeurodes vaporariorum quantity statistics alive of blade before the test and listing mark.Within 5 days after dispenser, investigate and record the trialeurodes vaporariorum number alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group is repeated meter of averaging, and calculate Revision insect recluced rate and preventive effect, its result is as shown in table 10.
Table 10
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| MM-01 | 144 | 44 | 69.44 | 74.08 |
| MM-02 | 123 | 30 | 75.61 | 79.31 |
| MM-03 | 147 | 34 | 76.87 | 80.38 |
| MM-04 | 124 | 30 | 75.81 | 79.48 |
| MM-05 | 137 | 36 | 73.72 | 77.71 |
| MM-06 | 142 | 39 | 72.54 | 76.70 |
| MM-07 | 167 | 73 | 56.29 | 62.92 |
| MM-08 | 166 | 62 | 62.65 | 68.32 |
| MM-09 | 161 | 58 | 63.98 | 69.44 |
| MM-10 | 185 | 69 | 62.70 | 68.36 |
| MM-11 | 137 | 54 | 60.58 | 66.56 |
| MM-12 | 149 | 62 | 58.39 | 64.70 |
| D1 | 142 | 123 | 13.38 | 26.52 |
| D2 | 146 | 131 | 10.27 | 23.89 |
| CK-02 | 123 | 145 | -17.89 | —— |
As can be seen from Table 10, product of the present invention has good preventive effect to trialeurodes vaporariorum, and better, visible than the control efficiency of single extract from Lantana camara and Azolla imbricata extract, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.In addition, have no during dispenser and have poisoning to show to crop.
Test example 6 the present invention contains the efficiency test of the composition pesticide oranges and tangerines cottonycushion scale of lantana and Azolla imbricata extract
Product MM-01 ~ MM-12 of the present invention is used for preventing and treating oranges and tangerines cottonycushion scale.This test 12 medicaments, 2 single doses, 1 blank, repeats for three times, Gong45Ge community.Minizone and surrounding all leave isolation strip, plot area 10m
2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the cottonycushion scale quantity statistics alive of blade before the test and listing mark.Within 5 days after dispenser, investigate and record the cottonycushion scale number alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group is repeated meter of averaging, and calculate Revision insect recluced rate and preventive effect, its result is as shown in table 11.
Table 11
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| MM-01 | 222 | 73 | 67.12 | 71.39 |
| MM-02 | 234 | 59 | 74.79 | 78.06 |
| MM-03 | 253 | 60 | 76.28 | 79.37 |
| MM-04 | 251 | 63 | 74.90 | 78.16 |
| MM-05 | 285 | 80 | 71.93 | 75.58 |
| MM-06 | 296 | 87 | 70.61 | 74.43 |
| MM-07 | 259 | 116 | 55.21 | 61.04 |
| MM-08 | 251 | 95 | 62.15 | 67.07 |
| MM-09 | 241 | 87 | 63.90 | 68.59 |
Continued 11
| MM-10 | 266 | 100 | 62.41 | 67.29 |
| MM-11 | 284 | 117 | 58.80 | 64.16 |
| MM-12 | 292 | 122 | 58.22 | 63.65 |
| D1 | 237 | 205 | 13.50 | 24.75 |
| D2 | 235 | 213 | 9.36 | 21.15 |
| CK-02 | 281 | 323 | -14.95 | —— |
As can be seen from Table 11, product of the present invention has good preventive effect to cottonycushion scale, and better, visible than the control efficiency of single extract from Lantana camara and Azolla imbricata extract, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.In addition, have no during dispenser and have poisoning to show to crop.
Claims (25)
1. contain the composition pesticide of lantana and Azolla imbricata extract, it is characterized in that, its active component is extracted by the crude drug of following weight portion and forms: lantana 1 part, Azolla imbricata 0.2 ~ 4 part, and active component is got by organic solvent or water extraction and formed, described organic solvent is methyl alcohol, ethanol, ethyl acetate, acetone or chloroform.
2. the composition pesticide containing lantana and Azolla imbricata extract according to claim 1, is characterized in that, its active component is extracted by the crude drug of following weight portion and forms: lantana 1 part, Azolla imbricata 0.5 ~ 2 part.
3. the composition pesticide containing lantana and Azolla imbricata extract according to claim 2, is characterized in that, its active component is extracted by the crude drug of following weight portion and forms: lantana 1 part, Azolla imbricata 0.5 ~ 1.5 part.
4. the composition pesticide containing lantana and Azolla imbricata extract according to claim 3, is characterized in that, its active component is extracted by the crude drug of following weight portion and forms: lantana 1 part, Azolla imbricata 1 part.
5. the composition pesticide containing lantana and Azolla imbricata extract according to any one of Claims 1 to 4, is characterized in that: after its active component is mixed by lantana and Azolla imbricata, common extraction forms; Or extract by lantana the extract from Lantana camara, the Azolla imbricata that obtain and extract the Azolla imbricata extract obtained and mix.
6. the composition pesticide containing lantana and Azolla imbricata extract according to any one of Claims 1 to 4, is characterized in that: the described composition pesticide containing lantana and Azolla imbricata extract is microemulsion, aqua or soluble concentrate.
7. the composition pesticide containing lantana and Azolla imbricata extract according to claim 5, is characterized in that: the described composition pesticide containing lantana and Azolla imbricata extract is microemulsion, aqua or soluble concentrate.
8. the composition pesticide containing lantana and Azolla imbricata extract according to any one of Claims 1 to 4, it is characterized in that: described containing in the composition pesticide of lantana and Azolla imbricata extract also comprises auxiliary agent, and described auxiliary agent is at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
9. the composition pesticide containing lantana and Azolla imbricata extract according to claim 5, it is characterized in that: described containing in the composition pesticide of lantana and Azolla imbricata extract also comprises auxiliary agent, and described auxiliary agent is at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
10. the composition pesticide containing lantana and Azolla imbricata extract according to claim 6, it is characterized in that: described containing in the composition pesticide of lantana and Azolla imbricata extract also comprises auxiliary agent, and described auxiliary agent is at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
11. composition pesticides containing lantana and Azolla imbricata extract according to claim 7, it is characterized in that: described containing in the composition pesticide of lantana and Azolla imbricata extract also comprises auxiliary agent, and described auxiliary agent is at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
12. composition pesticides containing lantana and Azolla imbricata extract according to claim 6, it is characterized in that: described soluble concentrate is made up of the component of following weight portion: lantana and Azolla imbricata extract, 10 parts of emulsifier 602,40 parts of ethanol, wherein, lantana and Azolla imbricata extract be 50 parts crude drug extract form.
13. composition pesticides containing lantana and Azolla imbricata extract according to claim 7, it is characterized in that: described soluble concentrate is made up of the component of following weight portion: lantana and Azolla imbricata extract, 10 parts of emulsifier 602,40 parts of ethanol, wherein, lantana and Azolla imbricata extract be 50 parts crude drug extract form.
14. composition pesticides containing lantana and Azolla imbricata extract according to claim 10, it is characterized in that: described soluble concentrate is made up of the component of following weight portion: lantana and Azolla imbricata extract, 10 parts of emulsifier 602,40 parts of ethanol, wherein, lantana and Azolla imbricata extract be 50 parts crude drug extract form.
15. composition pesticides containing lantana and Azolla imbricata extract according to claim 11, it is characterized in that: described soluble concentrate is made up of the component of following weight portion: lantana and Azolla imbricata extract, 10 parts of emulsifier 602,40 parts of ethanol, wherein, lantana and Azolla imbricata extract be 50 parts crude drug extract form.
16. composition pesticides containing lantana and Azolla imbricata extract according to claim 12, is characterized in that: lantana is prepared from by the following method with Azolla imbricata extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
17. composition pesticides containing lantana and Azolla imbricata extract according to claim 13, is characterized in that: lantana is prepared from by the following method with Azolla imbricata extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
18. composition pesticides containing lantana and Azolla imbricata extract according to claim 14, is characterized in that: lantana is prepared from by the following method with Azolla imbricata extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
19. composition pesticides containing lantana and Azolla imbricata extract according to claim 15, is characterized in that: lantana is prepared from by the following method with Azolla imbricata extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
The method containing the composition pesticide of lantana and Azolla imbricata extract described in 20. any one of preparation claim 1 ~ 19, is characterized in that being made up of following steps:
A, extraction: take lantana particle and Azolla imbricata particle, mixing, adds solvent by solid-liquid ratio 1:3 ~ 12g/ml, and in 60 ~ 95 DEG C of refluxing extraction, filter, obtain extract, wherein, described solvent is organic solvent or water;
B, preparation: extract is made microemulsion, soluble concentrate or aqua.
21. preparations according to claim 20 contain the method for the composition pesticide of lantana and Azolla imbricata extract, and it is characterized in that: in described a step, lantana particle and Azolla imbricata particle cross 60 ~ 100 mesh sieves; Solid-liquid ratio is 1:4 ~ 10g/ml; Organic solvent is methyl alcohol, ethanol, ethyl acetate, acetone, chloroform; Reflux temperature is 75 ~ 85 DEG C; Reflow's cycle is twice, and merging filtrate, obtains extract.
22. preparations according to claim 21 contain the method for the composition pesticide of lantana and Azolla imbricata extract, and it is characterized in that: in described a step, lantana particle and Azolla imbricata particle cross 80 mesh sieves; Solid-liquid ratio is 1:6g/ml; Organic solvent is absolute ethyl alcohol; Reflux temperature is 82 DEG C.
The application of the composition pesticide containing lantana and Azolla imbricata extract described in 23. any one of claim 1 ~ 19 in control crop pest, is characterized in that: described insect pest is Lepidoptera or homoptera pest.
24. application of composition pesticide in control crop pest containing lantana and Azolla imbricata extract according to claim 23, is characterized in that: during application, are diluted with water to the activity component concentration of crude drug weighing scale as 10 ~ 100ppm.
25. application of composition pesticide in control crop pest containing lantana and Azolla imbricata extract according to claim 24, is characterized in that: be diluted with water to the activity component concentration of crude drug weighing scale as 20 ~ 50ppm.
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| CN201310745702.8A CN103689002B (en) | 2013-12-30 | 2013-12-30 | Pesticide composition containing lantana camara and azolla imbricata extracts, preparation method and application |
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| CN115212276B (en) * | 2022-07-14 | 2023-11-21 | 集美大学 | A topical Chinese herbal composition for preventing and treating ciliate disease of shrimp and crab |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101715793A (en) * | 2009-10-30 | 2010-06-02 | 茹晖 | Ecological pesticide and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101715793A (en) * | 2009-10-30 | 2010-06-02 | 茹晖 | Ecological pesticide and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 伴生蕨类植物满江红提取物对水稻生长的影响;周兵;《热带作物学报》;20111231;第32卷(第7期);1229-1234 * |
| 马缨丹提取液对杨梦尼夜蛾和春尺蠖的活性测定;张志田等;《林业科技》;20130131;第38卷(第1期);40-41、28 * |
| 马缨丹提取物对美洲斑潜蝇成虫的驱避作用;任立云等;《华南农业大学学报》;20050131;第26卷(第1期);54-59 * |
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