CN103689002A - Pesticide composite containing lantana extract and azolla imbricata extract, preparation method and application - Google Patents
Pesticide composite containing lantana extract and azolla imbricata extract, preparation method and application Download PDFInfo
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- CN103689002A CN103689002A CN201310745702.8A CN201310745702A CN103689002A CN 103689002 A CN103689002 A CN 103689002A CN 201310745702 A CN201310745702 A CN 201310745702A CN 103689002 A CN103689002 A CN 103689002A
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Abstract
The invention relates to a pesticide composite containing a lantana extract and an azolla imbricata extract, a preparation method and an application, and belongs to the field of pesticides. The invention aims at providing the pesticide composite containing the lantana extract and the azolla imbricata extract, the preparation method and the application. Active components of the pesticide composite containing the lantana extract and the azolla imbricata extract are extracted by the following raw medicinal materials in parts by weight: 1 part of lantana and 0.2-4 parts of azolla imbricata; the active components of the pesticide composite can be coextracted after the raw medicinal materials, namely the lantana and the azolla imbricata are mixed; or the active components can be formed by separately extracting the lantana, obtaining the lantana extract, separately extracting the azolla imbricata, obtaining the azolla imbricata extract, and then mixing the two extracts. The pesticide composite containing the lantana extract and the azolla imbricata extract has an obvious control effect on lepidoptera and homoptera injurious insects, and has positive significance in agricultural production.
Description
Technical field
The present invention relates to composition pesticide and preparation method and application containing lantana and Azolla imbricata extract, belong to pesticide field.
Background technology
In recent years, in agriculture and forestry field, due to single drug and Scientific Usage of Drugs not, plant pest has produced pesticide resistance in various degree, and agriculture and forestry are produced still with chemical pesticide as mainly preventing and treating kind, have caused the destruction increasingly of environmental pollution and ecotope.Along with the raising of people's living standard and the enhancing of health perception, the development of biopesticide and exploitation just progressively receive agricultural chemicals R&D worker's concern, become the focus of agriculture and forestry sustainable development, simultaneously, also be to improve environment, the effective way of minimizing or delaying drug resistance and raising drug effect.Wherein, botanical pesticide has become one of focus of biopesticide research.
Lantana, is again Lantana camara, stinkweed, grass as one wishes.Belonging to Verenaceae Lantana, is perennial upright or half rattan shape shrub.Lantana originates in american torrid zone area, and all there is distribution the provinces and regions such as China Guangdong, Guangxi, Yunnan, Fujian, Taiwan.Except normal, cultivate in flower garden for viewing and admiring, root, leaf, flower also can be used as medicine, and have the effect of heat-clearing, removing toxic substances, hemostasis.
Azolla imbricata, the plant of Azolla imbricata section azolla, is grown in the small-sized floating plant in paddy field or pond.During children, be green, growth rapidly, grows up to a slice on the water surface of being everlasting.In time autumn and winter, its leaf contains a lot of anthocyan, and colony presents a slice redness, so be called Azolla imbricata.Azolla imbricata can all herbal medicine, the promoting eruption of inducing sweat, expelling wind and removing dampness.Can be used for measles without adequate eruption, rheumatic arthritis, nettle rash, in the treatment of the diseases such as pruitus.
In test, the present inventor finds that lantana and the common extract of Azolla imbricata and extract from Lantana camara and Azolla imbricata extract mix and have good synergistic function, can be prepared into insecticide, cabbage caterpillar, diamond-back moth, aphid, trialeurodes vaporariorum etc. are had to good preventive effect.
Summary of the invention
The technical problem to be solved in the present invention is to provide composition pesticide and the preparation method and application containing lantana and Azolla imbricata extract.
The present invention is containing the composition pesticide of lantana and Azolla imbricata extract, and its active component is formed by the crude drug extraction of following weight portion: 1 part of lantana, 0.2～4 part of Azolla imbricata.Wherein, active component is preferably formed by the crude drug extraction of following weight portion: 1 part of lantana, 0.5～2 part of Azolla imbricata; The crude drug extraction being preferably again by following weight portion forms: 1 part of lantana, 0.5～1.5 part of Azolla imbricata; The more preferably crude drug extraction by following weight portion forms: 1 part of lantana, 1 part of Azolla imbricata.
Wherein, lantana and Azolla imbricata that the present invention adopts are Chinese herbal medicine, and in order to improve the present invention containing the activity of the composition pesticide of lantana and Azolla imbricata extract, lantana is dry Leaves of Lantana Camara preferably, and Azolla imbricata is dry Azolla imbricata herb preferably.
The present invention is containing the composition pesticide of lantana and Azolla imbricata extract, and after its active component can be mixed by crude drug lantana and Azolla imbricata, common extraction forms; Also can first lantana be extracted and obtain extract from Lantana camara separately, Azolla imbricata extracts and obtains Azolla imbricata extract separately, then two kinds of extracts are mixed.In order to improve the insecticidal properties of composition pesticide of the present invention, after its active component is preferably and is mixed by lantana and Azolla imbricata, common extraction forms.
Further, in order to improve the stability containing the composition pesticide of lantana and Azolla imbricata extract, in the composition pesticide of the present invention containing lantana and Azolla imbricata extract, also comprise auxiliary agent, described auxiliary agent can adopt the usual auxiliaries in agricultural chemicals, as at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
Wherein, emulsifier can adopt at least one in non-ionic surface active agent and anion surfactant, described non-ionic surface active agent can be dibenzyl xenyl APEO, styryl phenyl APEO, styryl phenyl APEO, phenethyl phenyl polyoxyethylene one polyethenoxy ether, OPEO, alkylphenol polyoxyethylene sulfosuccinate, aliphatic acid polyethenoxy ether, fatty alcohol-polyoxyethylene ether, aliphatic amine polyoxyethylene ether, castor oil ethylene oxide adduct and derivative thereof, at least one in polyol fatty acid ester and ethylene oxide adduct etc. thereof, described anion surfactant is at least one in calcium dodecyl benzene sulfonate, alkylphenol polyoxyethylene phosphate, phenethyl phenol polyethenoxy ether phosphate etc., as emulsifier 500,601p, 602p etc., in addition, can also use commercially available emulsifier production.
Wherein, solvent can adopt at least one in water, ethanol, isopropyl alcohol, n-butanol, ethyl acetate, butyl acetate, methyl-sulfoxide, turpentine oil, solvent naphtha, 1-METHYLPYRROLIDONE, dimethyl formamide etc.
Wherein, antifreeze can be glycerine, propane diols, ethylene glycol, sodium chloride, calcium chloride, sorbierite etc.
Wherein, defoamer is silicone defoaming agent etc.
Wherein, dispersant is at least one in lignosulfonates, PAA and propionamide copolymer, alkylnaphthalene sulfonate, polycarboxylic acid salt's condensation product, methyl sodiosul foaliphatate etc.
Wherein, wetting agent can be alkyl sulfate, alkylbenzenesulfonate, alkylnaphthalene sulfonate, aliphatic alcohol polyethenoxy base ether, alkylphenol-polyethenoxy base ether, sorbitan fatty acid ester etc.
Wherein, stabilizing agent can be 2-expoxy propane, butyl glycidyl ether, isopropyl alcohol, n-butanol, polyethylene glycol etc.
The present invention can be prepared into by the method for this area routine containing the composition pesticide of lantana and Azolla imbricata extract any one formulation that is applicable to agricultural use, for the ease of storing and transportation, can make microemulsion, aqua or soluble concentrate.
Further, be preferably the soluble concentrate that the component by following weight portion forms: lantana and Azolla imbricata extract, 602,40 parts of ethanol of 10 parts of emulsifier, wherein, lantana and Azolla imbricata extract are that the crude drug of 50 parts extracts and forms.
For the ease of storing and transportation, can extract is concentrated, obtain concentrate, and then be prepared into various formulations, cycles of concentration can be adjusted voluntarily according to needs of production, as preferred version, lantana is prepared from by the following method with Azolla imbricata extract: crude drug is identical with crude drug weight through extracting and being concentrated into.
Second technical problem that the present invention will solve is to provide preparation containing the method for the composition pesticide of lantana and Azolla imbricata extract.
The present invention is comprised of following steps containing the preparation method of the composition pesticide of lantana and Azolla imbricata extract:
A, extraction: take lantana particle and Azolla imbricata particle, mix, by solid-liquid ratio, 1:3～12g/ml adds solvent, in 60～95 ℃ of refluxing extraction, filter, and obtains extract, and wherein, described solvent is organic solvent or water;
B, preparation: extract is made to microemulsion, soluble concentrate or aqua.
Wherein, lantana particle and Azolla imbricata particle can adopt commercially available particle, also can adopt conventional method pulverizing Leaves of Lantana Camara and Azolla imbricata herb to obtain.In order to improve the effect of extraction, simultaneously cost-saving, lantana particle and Azolla imbricata particle are crossed 60～100 mesh sieves, preferred mistake 80 mesh sieves.
Further, for cost-saving, simultaneously also in order to improve extraction efficiency, the solid-liquid ratio in a step is preferably 1:4～10g/ml, more preferably 1:6g/ml.The lantana and the Azolla imbricata particle that are lg can add 3～12ml solvent extraction, preferably add 4～10ml, more preferably add 6ml.
Conventional organic solvent all can be used for the present invention, for cost-saving, improves dissolution rate, guarantees extraction efficiency simultaneously, and organic solvent is preferably methyl alcohol, ethanol, ethyl acetate, acetone, chloroform, more preferably absolute ethyl alcohol.
In order to shorten preparation time, accelerate extraction rate, can when adding hot reflux, stir, mixing speed is preferably 80～100 revs/min.
Further, reflux temperature is preferably 75～85 ℃, more preferably 82 ℃; Reflow's cycle is twice, and each return time is 2.5 hours, and merging filtrate, obtains extract.
The present invention also provides the application in control crop pest of composition pesticide that the present invention contains lantana and Azolla imbricata extract.The present invention is containing the composition pesticide of lantana and Azolla imbricata extract, to lepidoptera pest as cabbage caterpillar, diamond-back moth, prodenia litura, beet snout moth's larva, tea tree tea geometrid; Homoptera pest has good preventive effect as aphid, scale insect, trialeurodes vaporariorum etc.
In actual agricultural production, in order to improve composition pesticide containing lantana and the Azolla imbricata extract control efficiency to insect pest, the described composition pesticide containing lantana and Azolla imbricata extract is preferably applied to crop pest initial stage of origination; The present invention can use by common method containing the composition pesticide of lantana and Azolla imbricata extract, sprays on crop blade face.As preferred version, while using, the present invention is diluted with water to containing the composition pesticide of lantana and Azolla imbricata extract to take the activity component concentration of crude drug weighing scale be 10～100ppm, it is the composition pesticide making after 1g crude drug extracts, when using, be diluted with water to 10kg, its activity component concentration equals 100ppm.Further preferably being diluted to activity component concentration is that 20～50ppm sprays in plant blade face, and to occur water membrane on blade face, not lower of liquid is advisable; After being preferable over 3～5 days, carry out secondary dispenser, the preferred continuous administration of a season crop 2～3 times.
The present invention is mixed by extract from Lantana camara and Azolla imbricata extract containing the composition pesticide of lantana and Azolla imbricata extract, or formed by lantana and common extraction of Azolla imbricata, its preventive effect is far away higher than the extract of single component, particularly lantana and Azolla imbricata are mixed to rear common extraction, owing to having solubilising, hydrotropy effect between lantana and Azolla imbricata, the composition pesticide insecticidal effect of making after common extraction is more remarkable.
Compared with prior art, the present invention has the following advantages containing the composition pesticide of lantana and Azolla imbricata extract:
1, the present invention has synergistic function containing the composition pesticide of lantana and Azolla imbricata extract, obvious to the control efficiency of Lepidoptera, homoptera pest.
2, lantana of the present invention and Azolla imbricata are common extracts or extracts respectively rear mixing, and the composition pesticide of making can improve crop immune system ability in quick-acting tagging, and can significantly improve its vigor.
3, the present invention be take Chinese herbal medicine as raw material, thereby does not have Pesticide Residue, and insect is difficult to produce resistance; Compare with independent use chemical source agricultural chemicals, environmentally friendly.
When 4, the present invention uses containing the composition pesticide of lantana and Azolla imbricata extract, valid density is low, with low cost.
Embodiment
The present invention is containing the composition pesticide of lantana and Azolla imbricata extract, and its active component is formed by the crude drug extraction of following weight portion: 1 part of lantana, 0.2～4 part of Azolla imbricata.Wherein, active component is preferably formed by the crude drug extraction of following weight portion: 1 part of lantana, 0.5～2 part of Azolla imbricata; The crude drug extraction being preferably again by following weight portion forms: 1 part of lantana, 0.5～1.5 part of Azolla imbricata; The more preferably crude drug extraction by following weight portion forms: 1 part of lantana, 1 part of Azolla imbricata.
Wherein, lantana and Azolla imbricata that the present invention adopts are Chinese herbal medicine, and in order to improve the present invention containing the activity of the composition pesticide of lantana and Azolla imbricata extract, lantana is dry Leaves of Lantana Camara preferably, and Azolla imbricata is dry Azolla imbricata herb preferably.
The present invention is containing the composition pesticide of lantana and Azolla imbricata extract, and after its active component can be mixed by crude drug lantana and Azolla imbricata, common extraction forms; Also can first lantana be extracted and obtain extract from Lantana camara separately, Azolla imbricata extracts and obtains Azolla imbricata extract separately, then two kinds of extracts are mixed.In order to improve the insecticidal properties of composition pesticide of the present invention, after its active component is preferably and is mixed by lantana and Azolla imbricata, common extraction forms.
Further, in order to improve the stability containing the composition pesticide of lantana and Azolla imbricata extract, in the composition pesticide of the present invention containing lantana and Azolla imbricata extract, also comprise auxiliary agent, described auxiliary agent can adopt the usual auxiliaries in agricultural chemicals, as at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
Wherein, emulsifier can adopt at least one in non-ionic surface active agent and anion surfactant, described non-ionic surface active agent can be dibenzyl xenyl APEO, styryl phenyl APEO, styryl phenyl APEO, phenethyl phenyl polyoxyethylene one polyethenoxy ether, OPEO, alkylphenol polyoxyethylene sulfosuccinate, aliphatic acid polyethenoxy ether, fatty alcohol-polyoxyethylene ether, aliphatic amine polyoxyethylene ether, castor oil ethylene oxide adduct and derivative thereof, at least one in polyol fatty acid ester and ethylene oxide adduct etc. thereof, described anion surfactant is at least one in calcium dodecyl benzene sulfonate, alkylphenol polyoxyethylene phosphate, phenethyl phenol polyethenoxy ether phosphate etc., as emulsifier 500,601p, 602p etc., in addition, can also use commercially available emulsifier production.
Wherein, solvent can adopt at least one in water, ethanol, isopropyl alcohol, n-butanol, ethyl acetate, butyl acetate, methyl-sulfoxide, turpentine oil, solvent naphtha, 1-METHYLPYRROLIDONE, dimethyl formamide etc.
Wherein, antifreeze can be glycerine, propane diols, ethylene glycol, sodium chloride, calcium chloride, sorbierite etc.
Wherein, defoamer is silicone defoaming agent etc.
Wherein, dispersant is at least one in lignosulfonates, PAA and propionamide copolymer, alkylnaphthalene sulfonate, polycarboxylic acid salt's condensation product, methyl sodiosul foaliphatate etc.
Wherein, wetting agent can be alkyl sulfate, alkylbenzenesulfonate, alkylnaphthalene sulfonate, aliphatic alcohol polyethenoxy base ether, alkylphenol-polyethenoxy base ether, sorbitan fatty acid ester etc.
Wherein, stabilizing agent can be 2-expoxy propane, butyl glycidyl ether, isopropyl alcohol, n-butanol, polyethylene glycol etc.
The present invention can be prepared into by the method for this area routine containing the composition pesticide of lantana and Azolla imbricata extract any one formulation that is applicable to agricultural use, for the ease of storing and transportation, can make microemulsion, aqua or soluble concentrate.
Further, be preferably the soluble concentrate that the component by following weight portion forms: lantana and Azolla imbricata extract, 602,40 parts of ethanol of 10 parts of emulsifier, wherein, lantana and Azolla imbricata extract are that the crude drug of 50 parts extracts and forms.
For the ease of storing and transportation, can extract is concentrated, obtain concentrate, and then be prepared into various formulations, cycles of concentration can be adjusted voluntarily according to needs of production, as preferred version, lantana is prepared from by the following method with Azolla imbricata extract: crude drug is identical with crude drug weight through extracting and being concentrated into.
The present invention is comprised of following steps containing the preparation method of the composition pesticide of lantana and Azolla imbricata extract:
A, extraction: take lantana particle and Azolla imbricata particle, mix, by solid-liquid ratio, 1:3～12g/ml adds solvent, in 60～95 ℃ of refluxing extraction, filter, and obtains extract, and wherein, described solvent is organic solvent or water;
B, preparation: extract is made to microemulsion, soluble concentrate or aqua.
Wherein, lantana particle and Azolla imbricata particle can adopt commercially available particle, also can adopt conventional method pulverizing Leaves of Lantana Camara and Azolla imbricata herb to obtain.In order to improve the effect of extraction, simultaneously cost-saving, lantana particle and Azolla imbricata particle are crossed 60～100 mesh sieves, preferred mistake 80 mesh sieves.
Further, for cost-saving, simultaneously also in order to improve extraction efficiency, the solid-liquid ratio in a step is preferably 1:4～10g/ml, more preferably 1:6g/ml.The lantana and the Azolla imbricata particle that are lg can add 3～12ml solvent extraction, preferably add 4～10ml, more preferably add 6ml.
Conventional organic solvent all can be used for the present invention, for cost-saving, improves dissolution rate, guarantees extraction efficiency simultaneously, and organic solvent is preferably methyl alcohol, ethanol, ethyl acetate, acetone, chloroform, more preferably absolute ethyl alcohol.
In order to shorten preparation time, accelerate extraction rate, can when adding hot reflux, stir, mixing speed is preferably 80～100 revs/min.
Further, reflux temperature is preferably 75～85 ℃, more preferably 82 ℃; Reflow's cycle is twice, and each return time is 2.5 hours, and merging filtrate, obtains extract.
The present invention is containing the composition pesticide of lantana and Azolla imbricata extract, to lepidoptera pest as cabbage caterpillar, diamond-back moth, prodenia litura, beet snout moth's larva, tea tree tea geometrid; Homoptera pest has good preventive effect as aphid, scale insect, trialeurodes vaporariorum etc.
In actual agricultural production, in order to improve composition pesticide containing lantana and the Azolla imbricata extract control efficiency to insect pest, the described composition pesticide containing lantana and Azolla imbricata extract is preferably applied to crop pest initial stage of origination; The present invention can use by common method containing the composition pesticide of lantana and Azolla imbricata extract, sprays on crop blade face.As preferred version, while using, the present invention is diluted with water to containing the composition pesticide of lantana and Azolla imbricata extract to take the activity component concentration of crude drug weighing scale be 10～100ppm, it is the composition pesticide making after 1g crude drug extracts, when using, be diluted with water to 10kg, its activity component concentration equals 100ppm.Further preferably being diluted to activity component concentration is that 20～50ppm sprays in plant blade face, and to occur water membrane on blade face, not lower of liquid is advisable; After being preferable over 3～5 days, carry out secondary dispenser, the preferred continuous administration of a season crop 2～3 times.
The present invention is mixed by extract from Lantana camara and Azolla imbricata extract containing the composition pesticide of lantana and Azolla imbricata extract, or formed by lantana and common extraction of Azolla imbricata, its preventive effect is far away higher than the extract of single component, particularly lantana and Azolla imbricata are mixed to rear common extraction, owing to having solubilising, hydrotropy effect between lantana and Azolla imbricata, the composition pesticide insecticidal effect of making after common extraction is more remarkable.
Below in conjunction with embodiment, the specific embodiment of the present invention is further described, does not therefore limit the present invention among described scope of embodiments.
Embodiment 1 is containing the preparation of the composition pesticide of lantana and Azolla imbricata extract
A, extraction: take dry Leaves of Lantana Camara and Azolla imbricata herb, mix, pulverize, cross 80 mesh sieves, add extraction solution, refluxing extraction twice, filters, and merging filtrate is concentrated, makes extract weight equal the weight of crude drug, obtains extract mm-01～mm-06; Wherein, each parameter is in Table 1;
B, preparation: auxiliary agent add table 2 in extract mm-01～mm-06 in, stir, must be containing the composition pesticide MM-01～MM-06 of lantana and extract from Lantana camara.
The preparation technology parameter of table 1 lantana and Azolla imbricata extract
Wherein, Leaves of Lantana Camara and Azolla imbricata herb are all purchased from Anguo City Zhong Tian prepared slices of Chinese crude drugs Co., Ltd.
Table 2 is containing the formula of the composition pesticide of lantana and Azolla imbricata extract
Embodiment 2 is containing the preparation of the composition pesticide of lantana and Azolla imbricata extract
A, extraction: take dry Leaves of Lantana Camara, pulverize, cross 80 mesh sieves, add extraction solution, refluxing extraction twice, filters, and merging filtrate is concentrated, makes extract weight equal the weight of crude drug, obtains extract d1-01～d1-03, and wherein, each parameter is in Table 3;
Take dry Azolla imbricata herb, pulverize, cross 80 mesh sieves, add extraction solution, refluxing extraction twice, filters, and merging filtrate is concentrated, makes extract weight equal the weight of crude drug, obtains extract d2-01～d2-03, and wherein, each parameter is in Table 4;
B, preparation: take lantana extract, Azolla imbricata extract and auxiliary agent, stirring and evenly mixing, obtains the composition pesticide MM-07～MM-12 containing lantana and Azolla imbricata extract, and wherein, each parameter is in Table 5.
Table 3
Table 4
Table 5
Test example 1 the present invention is containing the efficiency test of the composition pesticide wild cabbage diamond-back moth of lantana and Azolla imbricata extract
For clearly putting into field efficacy situation containing the composition pesticide of lantana and Azolla imbricata extract, inventor has carried out field control effectiveness test to this medicament.
1, test material
1.1 for studying thing and object: wild cabbage diamond-back moth
1.2 reagent agent
Product in embodiment: MM-01～MM-12;
Lantana single dose (D1), Azolla imbricata single dose (D2):
Chemical reagent contrast (CK-01): gamma cyhalothrin, purchased from the biochemical Co., Ltd of Chengdu Ke Lilong;
Clear water contrast (CK-02).
Wherein, the preparation method of lantana single dose is: get the d1-03 of 50g, add 10g emulsifier 602, and 40g ethanol, stirring and evenly mixing, makes lantana single dose.
The preparation method of Azolla imbricata single dose is: gets the d2-03 of 50g, adds 10g emulsifier 602, and 40g ethanol, stirring and evenly mixing, makes Azolla imbricata single dose.
2, experimental scheme and method
14 medicaments of this test, 1 contrast medicament, 1 blank, repeats Gong48Ge community for three times.Minizone and surrounding all leave isolation strip, community area 12m
2, randomized arrangement is respectively processed in community.
By reagent agent thin up, to activity component concentration, be 50ppm, spray application to plant blade face, to occur water membrane on blade face, not lower of liquid is advisable.
Before test, carry out diamond-back moth quantity statistics alive the listing mark of fixed blade.Within 5 days after dispenser, investigate and record the diamond-back moth number alive on each blade.Calculate insect population decline rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
3, result of the test
Within 5 days after dispenser, investigate and record the borer population alive on each blade, calculate insect population decline rate and preventive effect, it the results are shown in Table 6.
Table 6
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Insect population decline rate (%) | Preventive effect (%) |
| MM-01 | 215 | 47 | 78.14 | 82.35 |
| MM-02 | 255 | 34 | 86.67 | 89.23 |
| MM-03 | 264 | 32 | 87.88 | 90.21 |
| MM-04 | 243 | 32 | 86.83 | 89.37 |
| MM-05 | 232 | 36 | 84.48 | 87.47 |
| MM-06 | 226 | 41 | 81.86 | 85.35 |
| MM-07 | 267 | 83 | 68.91 | 74.90 |
| MM-08 | 286 | 73 | 74.48 | 79.39 |
| MM-09 | 295 | 72 | 75.59 | 80.29 |
Continued 6
| MM-10 | 257 | 65 | 74.71 | 79.58 |
| MM-11 | 241 | 65 | 73.03 | 78.22 |
| MM-12 | 235 | 67 | 71.49 | 76.98 |
| D1 | 257 | 212 | 17.51 | 33.39 |
| D2 | 259 | 220 | 15.06 | 31.41 |
| CK-01 | 233 | 23 | 90.13 | 92.03 |
| CK-02 | 214 | 265 | -23.83 | —— |
As can be seen from Table 6, product of the present invention has good preventive effect to diamond-back moth, and better, visible than the control efficiency of single extract from Lantana camara and Azolla imbricata extract, between product compositions of the present invention, has synergistic function.And the preventive effect that adopts the product that common extracting method makes is apparently higher than blended product after independent extraction, visible common extraction contributes to improve dissolution rate, improves insecticide efficiency.
Test example 2 the present invention are containing the efficiency test of the composition pesticide tea tree tea geometrid of lantana and Azolla imbricata extract
Product MM-01～MM-12 of the present invention is used for preventing and treating tea tree tea geometrid.12 medicaments of this test, 2 single doses, 1 blank, repeats Gong45Ge community for three times.Minizone and surrounding all leave isolation strip, community area 10m
2, randomized arrangement is respectively processed in community.
By reagent agent thin up, to activity component concentration, be 50ppm, spray application to plant blade face, to occur water membrane on blade face, not lower of liquid is advisable.
Before test, carry out tea geometrid quantity statistics alive the listing mark of fixed blade.Within 5 days after dispenser, investigate and record the tea geometrid number alive on each blade.Calculate insect population decline rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group meter that repeats to average, calculates insect population decline rate and preventive effect, and it the results are shown in Table 7.
Table 7
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Insect population decline rate (%) | Preventive effect (%) |
| MM-01 | 147 | 41 | 72.11 | 76.53 |
Continued 7
| MM-02 | 135 | 27 | 80.00 | 83.17 |
| MM-03 | 146 | 27 | 81.51 | 84.44 |
| MM-04 | 158 | 31 | 80.38 | 83.49 |
| MM-05 | 118 | 26 | 77.97 | 81.46 |
| MM-06 | 128 | 31 | 75.78 | 79.62 |
| MM-07 | 126 | 46 | 63.49 | 69.28 |
| MM-08 | 168 | 53 | 68.45 | 73.46 |
| MM-09 | 169 | 48 | 71.60 | 76.10 |
| MM-10 | 167 | 51 | 69.46 | 74.31 |
| MM-11 | 159 | 51 | 67.92 | 73.01 |
| MM-12 | 174 | 59 | 66.09 | 71.47 |
| D1 | 186 | 162 | 12.90 | 26.72 |
| D2 | 147 | 131 | 10.88 | 25.02 |
| CK-02 | 122 | 145 | -18.85 | —— |
As can be seen from Table 7, product of the present invention has good preventive effect to tea geometrid, and better, visible than the control efficiency of single extract from Lantana camara and Azolla imbricata extract, between product compositions of the present invention, has synergistic function.And the preventive effect that adopts the product that common extracting method makes is apparently higher than blended product after independent extraction, visible common extraction contributes to improve dissolution rate, improves insecticide efficiency.In addition, during dispenser, have no crop is had to poisoning performance.
Test example 3 the present invention are containing the efficiency test of the composition pesticide wild cabbage prodenia litura of lantana and Azolla imbricata extract
Product MM-01～MM-12 of the present invention is used for preventing and treating wild cabbage prodenia litura.12 medicaments of this test, 2 single doses, 1 blank, repeats Gong45Ge community for three times.Minizone and surrounding all leave isolation strip, community area 20m
2, randomized arrangement is respectively processed in community.
By reagent agent thin up, to activity component concentration, be 50ppm, spray application to plant blade face, to occur water membrane on blade face, not lower of liquid is advisable.
Before test, carry out prodenia litura quantity statistics alive the listing mark of fixed blade.Within 5 days after dispenser, investigate and record the prodenia litura number alive on each blade.Calculate insect population decline rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group meter that repeats to average, calculates insect population decline rate and preventive effect, and its result is as shown in table 8.
Table 8
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Insect population decline rate (%) | Preventive effect (%) |
| MM-01 | 224 | 57 | 74.55 | 78.26 |
| MM-02 | 245 | 49 | 80.00 | 82.91 |
| MM-03 | 253 | 43 | 83.00 | 85.48 |
| MM-04 | 265 | 50 | 81.13 | 83.88 |
| MM-05 | 274 | 60 | 78.10 | 81.29 |
| MM-06 | 216 | 50 | 76.85 | 80.22 |
| MM-07 | 285 | 106 | 62.81 | 68.22 |
| MM-08 | 298 | 91 | 69.46 | 73.91 |
| MM-09 | 289 | 84 | 70.93 | 75.17 |
| MM-10 | 247 | 75 | 69.64 | 74.06 |
| MM-11 | 263 | 86 | 67.30 | 72.06 |
| MM-12 | 285 | 97 | 65.96 | 70.92 |
| D1 | 212 | 185 | 12.74 | 25.45 |
| D2 | 244 | 218 | 10.66 | 23.67 |
| CK-02 | 217 | 254 | -17.05 | —— |
As can be seen from Table 8, product of the present invention has good preventive effect to prodenia litura, and better, visible than the control efficiency of single extract from Lantana camara and Azolla imbricata extract, between product compositions of the present invention, has synergistic function.And the preventive effect that adopts the product that common extracting method makes is apparently higher than blended product after independent extraction, visible common extraction contributes to improve dissolution rate, improves insecticide efficiency.In addition, during dispenser, have no crop is had to poisoning performance.
Test example 4 the present invention are containing the efficiency test of the composition pesticide pumpkin aphid of lantana and Azolla imbricata extract
Product MM-01～MM-12 of the present invention is used for preventing and treating pumpkin aphid.12 medicaments of this test, 2 single doses, 1 blank, repeats Gong45Ge community for three times.Minizone and surrounding all leave isolation strip, community area 10m
2, randomized arrangement is respectively processed in community.
By reagent agent thin up, to activity component concentration, be 50ppm, spray application to plant blade face, to occur water membrane on blade face, not lower of liquid is advisable.
Before test, carry out Aphed population statistics alive the listing mark of fixed blade.Within 5 days after dispenser, investigate and record the aphid number alive on each blade.Calculate insect population decline rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group meter that repeats to average, calculates insect population decline rate and preventive effect, and its result is as shown in table 9.
Table 9
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Insect population decline rate (%) | Preventive effect (%) |
| MM-01 | 422 | 84 | 80.09 | 82.10 |
| MM-02 | 431 | 58 | 86.54 | 87.90 |
| MM-03 | 441 | 54 | 87.76 | 88.99 |
| MM-04 | 453 | 60 | 86.75 | 88.09 |
| MM-05 | 465 | 70 | 84.95 | 86.46 |
| MM-06 | 457 | 76 | 83.37 | 85.04 |
| MM-07 | 485 | 138 | 71.55 | 74.41 |
| MM-08 | 447 | 108 | 75.84 | 78.27 |
| MM-09 | 497 | 111 | 77.67 | 79.91 |
| MM-10 | 406 | 97 | 76.11 | 78.51 |
| MM-11 | 437 | 111 | 74.60 | 77.15 |
| MM-12 | 424 | 112 | 73.58 | 76.24 |
| D1 | 418 | 306 | 26.79 | 34.16 |
| D2 | 426 | 326 | 23.47 | 31.17 |
| CK-02 | 447 | 497 | -11.19 | —— |
As can be seen from Table 9, product of the present invention has good preventive effect to aphid, and better, visible than the control efficiency of single extract from Lantana camara and Azolla imbricata extract, between product compositions of the present invention, has synergistic function.And the preventive effect that adopts the product that common extracting method makes is apparently higher than blended product after independent extraction, visible common extraction contributes to improve dissolution rate, improves insecticide efficiency.In addition, during dispenser, have no crop is had to poisoning performance.
Test example 5 the present invention are containing the efficiency test of the composition pesticide eggplant trialeurodes vaporariorum of lantana and Azolla imbricata extract
Product MM-01～MM-12 of the present invention is used for preventing and treating eggplant trialeurodes vaporariorum.12 medicaments of this test, 2 single doses, 1 blank, repeats Gong45Ge community for three times.Minizone and surrounding all leave isolation strip, community area 10m
2, randomized arrangement is respectively processed in community.
By reagent agent thin up, to activity component concentration, be 50ppm, spray application to plant blade face, to occur water membrane on blade face, not lower of liquid is advisable.
Before test, carry out trialeurodes vaporariorum quantity statistics alive the listing mark of fixed blade.Within 5 days after dispenser, investigate and record the trialeurodes vaporariorum number alive on each blade.Calculate insect population decline rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group meter that repeats to average, calculates insect population decline rate and preventive effect, and its result is as shown in table 10.
Table 10
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Insect population decline rate (%) | Preventive effect (%) |
| MM-01 | 144 | 44 | 69.44 | 74.08 |
| MM-02 | 123 | 30 | 75.61 | 79.31 |
| MM-03 | 147 | 34 | 76.87 | 80.38 |
| MM-04 | 124 | 30 | 75.81 | 79.48 |
| MM-05 | 137 | 36 | 73.72 | 77.71 |
| MM-06 | 142 | 39 | 72.54 | 76.70 |
| MM-07 | 167 | 73 | 56.29 | 62.92 |
| MM-08 | 166 | 62 | 62.65 | 68.32 |
| MM-09 | 161 | 58 | 63.98 | 69.44 |
| MM-10 | 185 | 69 | 62.70 | 68.36 |
| MM-11 | 137 | 54 | 60.58 | 66.56 |
| MM-12 | 149 | 62 | 58.39 | 64.70 |
| D1 | 142 | 123 | 13.38 | 26.52 |
| D2 | 146 | 131 | 10.27 | 23.89 |
| CK-02 | 123 | 145 | -17.89 | —— |
As can be seen from Table 10, product of the present invention has good preventive effect to trialeurodes vaporariorum, and better, visible than the control efficiency of single extract from Lantana camara and Azolla imbricata extract, between product compositions of the present invention, has synergistic function.And the preventive effect that adopts the product that common extracting method makes is apparently higher than blended product after independent extraction, visible common extraction contributes to improve dissolution rate, improves insecticide efficiency.In addition, during dispenser, have no crop is had to poisoning performance.
Test example 6 the present invention are containing the efficiency test of the composition pesticide oranges and tangerines cottonycushion scale of lantana and Azolla imbricata extract
Product MM-01～MM-12 of the present invention is used for preventing and treating oranges and tangerines cottonycushion scale.12 medicaments of this test, 2 single doses, 1 blank, repeats Gong45Ge community for three times.Minizone and surrounding all leave isolation strip, community area 10m
2, randomized arrangement is respectively processed in community.
By reagent agent thin up, to activity component concentration, be 50ppm, spray application to plant blade face, to occur water membrane on blade face, not lower of liquid is advisable.
Before test, carry out cottonycushion scale quantity statistics alive the listing mark of fixed blade.Within 5 days after dispenser, investigate and record the cottonycushion scale number alive on each blade.Calculate insect population decline rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group meter that repeats to average, calculates insect population decline rate and preventive effect, and its result is as shown in table 11.
Table 11
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Insect population decline rate (%) | Preventive effect (%) |
| MM-01 | 222 | 73 | 67.12 | 71.39 |
| MM-02 | 234 | 59 | 74.79 | 78.06 |
| MM-03 | 253 | 60 | 76.28 | 79.37 |
| MM-04 | 251 | 63 | 74.90 | 78.16 |
| MM-05 | 285 | 80 | 71.93 | 75.58 |
| MM-06 | 296 | 87 | 70.61 | 74.43 |
| MM-07 | 259 | 116 | 55.21 | 61.04 |
| MM-08 | 251 | 95 | 62.15 | 67.07 |
| MM-09 | 241 | 87 | 63.90 | 68.59 |
Continued 11
| MM-10 | 266 | 100 | 62.41 | 67.29 |
| MM-11 | 284 | 117 | 58.80 | 64.16 |
| MM-12 | 292 | 122 | 58.22 | 63.65 |
| D1 | 237 | 205 | 13.50 | 24.75 |
| D2 | 235 | 213 | 9.36 | 21.15 |
| CK-02 | 281 | 323 | -14.95 | —— |
As can be seen from Table 11, product of the present invention has good preventive effect to cottonycushion scale, and better, visible than the control efficiency of single extract from Lantana camara and Azolla imbricata extract, between product compositions of the present invention, has synergistic function.And the preventive effect that adopts the product that common extracting method makes is apparently higher than blended product after independent extraction, visible common extraction contributes to improve dissolution rate, improves insecticide efficiency.In addition, during dispenser, have no crop is had to poisoning performance.
Claims (10)
1. containing the composition pesticide of lantana and Azolla imbricata extract, it is characterized in that, its active component is formed by the crude drug extraction of following weight portion: 1 part of lantana, 0.2～4 part of Azolla imbricata.
2. the composition pesticide containing lantana and Azolla imbricata extract according to claim 1, is characterized in that, its active component is formed by the crude drug extraction of following weight portion: 1 part of lantana, 0.5～2 part of Azolla imbricata; Be preferably 1 part of lantana, 0.5～1.5 part of Azolla imbricata; More preferably lantana is 1 part, 1 part of Azolla imbricata.
3. the composition pesticide containing lantana and Azolla imbricata extract according to claim 1 and 2, is characterized in that: after its active component is mixed by lantana and Azolla imbricata, common extraction forms; Or by lantana, extract the extract from Lantana camara, the Azolla imbricata that obtain and extract the Azolla imbricata extract obtaining and mix.
4. according to the composition pesticide containing lantana and Azolla imbricata extract described in claim 1～3 any one, it is characterized in that: the described composition pesticide containing lantana and Azolla imbricata extract is microemulsion, aqua or soluble concentrate.
5. according to the composition pesticide containing lantana and Azolla imbricata extract described in claim 1～4 any one, it is characterized in that: in the described composition pesticide containing lantana and Azolla imbricata extract, also comprise auxiliary agent, described auxiliary agent is at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
6. the composition pesticide containing lantana and Azolla imbricata extract according to claim 5, it is characterized in that: described soluble concentrate is comprised of the component of following weight portion: lantana and Azolla imbricata extract, 10 parts of emulsifier 602,40 parts of ethanol, wherein, lantana and Azolla imbricata extract are that the crude drug of 50 parts extracts and to form; Lantana is preferably prepared from by the following method with Azolla imbricata extract: crude drug is identical with crude drug weight through extracting and being concentrated into.
7. the method for the composition pesticide containing lantana and Azolla imbricata extract described in preparation claim 1～6 any one, is characterized in that being comprised of following steps:
A, extraction: take lantana particle and Azolla imbricata particle, mix, by solid-liquid ratio, 1:3～12g/ml adds solvent, in 60～95 ℃ of refluxing extraction, filter, and obtains extract, and wherein, described solvent is organic solvent or water;
B, preparation: extract is made to microemulsion, soluble concentrate or aqua.
8. preparation according to claim 7, containing the method for the composition pesticide of lantana and Azolla imbricata extract, is characterized in that: in described a step, lantana particle and Azolla imbricata particle are crossed 60～100 mesh sieves preferred mistake 80 mesh sieves; Solid-liquid ratio is 1:4～10g/ml, is preferably 1:6g/ml; Organic solvent is methyl alcohol, ethanol, ethyl acetate, acetone, chloroform, is preferably absolute ethyl alcohol; Reflux temperature is 75～85 ℃, is preferably 82 ℃; Reflow's cycle is twice, and merging filtrate, obtains extract.
9. the application of the composition pesticide containing lantana and Azolla imbricata extract described in claim 1～6 any one in control crop pest.
10. the application of the composition pesticide containing lantana and Azolla imbricata extract according to claim 9 in control crop pest, is characterized in that: described insect pest is Lepidoptera, homoptera pest; During application, be diluted with water to that to take the activity component concentration of crude drug weighing scale be 10～100ppm, be preferably diluted with water to that to take the activity component concentration of crude drug weighing scale be 20～50ppm.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104000907A (en) * | 2014-06-16 | 2014-08-27 | 苏鹏 | Traditional Chinese medicine for treating chronic urticaria and preparation method thereof |
| CN115212276A (en) * | 2022-07-14 | 2022-10-21 | 集美大学 | External Chinese herbal medicine composition for preventing and treating shrimp and crab ciliate diseases |
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| CN101715793A (en) * | 2009-10-30 | 2010-06-02 | 茹晖 | Ecological pesticide and preparation method thereof |
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| CN101715793A (en) * | 2009-10-30 | 2010-06-02 | 茹晖 | Ecological pesticide and preparation method thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104000907A (en) * | 2014-06-16 | 2014-08-27 | 苏鹏 | Traditional Chinese medicine for treating chronic urticaria and preparation method thereof |
| CN104000907B (en) * | 2014-06-16 | 2015-10-28 | 苏鹏 | A kind of Chinese medicine for the treatment of chronic urticaria and preparation method thereof |
| CN115212276A (en) * | 2022-07-14 | 2022-10-21 | 集美大学 | External Chinese herbal medicine composition for preventing and treating shrimp and crab ciliate diseases |
| CN115212276B (en) * | 2022-07-14 | 2023-11-21 | 集美大学 | A topical Chinese herbal composition for preventing and treating ciliate disease of shrimp and crab |
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