CN103704285B - Pesticide composition containing extracts of veratrum nigrum and mamee-apple and preparation method and application of pesticide combination - Google Patents
Pesticide composition containing extracts of veratrum nigrum and mamee-apple and preparation method and application of pesticide combination Download PDFInfo
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- CN103704285B CN103704285B CN201310745528.7A CN201310745528A CN103704285B CN 103704285 B CN103704285 B CN 103704285B CN 201310745528 A CN201310745528 A CN 201310745528A CN 103704285 B CN103704285 B CN 103704285B
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Abstract
The invention relates to a pesticide composition containing extracts of veratrum nigrum and mamee-apple and a preparation method and application of the pesticide combination and belongs to the field of pesticides. Active components of the pesticide composition containing the extracts of the veratrum nigrum and the mamee-apple are obtained by extracting the following raw medicinal materials in parts by weight: 1 part of the veratrum nigrum and 0.5-6 parts of the mamee-apple. The active components of the pesticide composition are obtained by mixing the veratrum nigrum and the mamee-apple and then extracting the mixture or obtained by independently extracting the veratrum nigrum to obtain a veratrum nigrum extract, independently extracting the mamee-apple to obtain a mamee-apple extract and then mixing the two extracts. The pesticide composition containing the extracts of the veratrum nigrum and the mamee-apple has an obvious effect of preventing and treating homoptera, lepidoptera and acarina pests and has a positive significance for the agricultural production.
Description
Technical field
The present invention relates to the composition pesticide containing black false hellebore and Ma meter apricot extract and preparation method and application, belong to pesticide field.
Background technology
In recent years, in agriculture and forestry field, due to single drug and not Scientific Usage of Drugs, plant pest has produced pesticide resistance in various degree, and agriculture and forestry produce still with chemical pesticide for mainly to prevent and treat kind, cause the destruction increasingly of environmental pollution and ecotope.Along with the raising of people's living standard and the enhancing of health perception, the manufacture and exploit of biopesticide just progressively receives the concern of agricultural chemicals R&D worker, becomes the focus of agriculture and forestry sustainable development, simultaneously, also be environmental protect, the effective way of minimizing or delaying drug resistance and raising drug effect.Wherein, botanical pesticide has become one of focus of biopesticide research.
Black false hellebore, the plant of Liliaceae Veratrum.Containing multiple bioactive ingredients such as jervine, jervine, false jervine, Rubijevine, colchicin, germerine and veratroylzygadenines in black false hellebore, find after deliberation, black false hellebore can be eliminated the phlegm, emetic, desinsection.Can be used for the diseases such as the heap soil or fertilizer over and around the roots for the treatment of apoplexy phlegm, epilepsy, angioleucitis, malaria, mastitis, fracture, traumatic injury, favus of the scalp, scabies, also can be used for go out maggot, fly etc.
Ma meter Xing is the plant that the full U.S. fruit of Hypericaceae belongs to.In test, the present inventor finds that black false hellebore and the common extract of Ma meter Xing and black false hellebore extract and the mixing of Ma meter apricot extract have good synergistic function, can insecticide be prepared into, have good preventive effect to aphid, scale insect, tetranychid, trialeurodes vaporariorum, cabbage caterpillar, diamond-back moth, prodenia litura etc.
Summary of the invention
The technical problem to be solved in the present invention is to provide composition pesticide containing black false hellebore and Ma meter apricot extract and preparation method and application.
The present invention contains the composition pesticide of black false hellebore and Ma meter apricot extract, and its active component is extracted by the crude drug of following weight portion and forms: black false hellebore 1 part, Ma meter Xing 0.5 ~ 6 part.Wherein, active component is preferably extracted by the crude drug of following weight portion and forms: black false hellebore 1 part, Ma meter Xing 1 ~ 4 part; Be preferably again and formed by the crude drug extraction of following weight portion: black false hellebore 1 part, Ma meter Xing 1 ~ 3 part; Be more preferably and formed by the crude drug extraction of following weight portion: black false hellebore 1 part, Ma meter Xing 2 parts.
Wherein, the black false hellebore that the present invention adopts and Ma meter Xing are Chinese herbal medicine, contain the activity of the composition pesticide of black false hellebore and Ma meter apricot extract in order to improve the present invention, the black false hellebore rhizome that black false hellebore is preferably dry, the Ma meter Xing leaf that Ma meter Xing is preferably dry.
The present invention contains the composition pesticide of black false hellebore and Ma meter apricot extract, and after its active component can be mixed by crude drug black false hellebore and Ma meter Xing, common extraction forms; Also first can be extracted separately by black false hellebore and obtain black false hellebore extract, Ma meter Xing extracts separately and obtains horse rice apricot extract, then is mixed by two kinds of extracts.In order to improve the insecticidal properties of composition pesticide of the present invention, after its active component is preferably and is mixed by black false hellebore and Ma meter Xing, common extraction forms.
Further, in order to improve the stability of the composition pesticide containing black false hellebore and Ma meter apricot extract, the present invention contains in the composition pesticide of black false hellebore and Ma meter apricot extract and also comprises auxiliary agent, described auxiliary agent can adopt the usual auxiliaries in agricultural chemicals, as at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
Wherein, emulsifier can adopt at least one in non-ionic surface active agent and anion surfactant, described non-ionic surface active agent can be dibenzyl xenyl APEO, styryl phenyl APEO, styryl phenyl APEO, phenethyl phenyl polyoxyethylene one polyethenoxy ether, OPEO, alkylphenol polyoxyethylene sulfosuccinate, aliphatic acid polyethenoxy ether, fatty alcohol-polyoxyethylene ether, aliphatic amine polyoxyethylene ether, castor oil ethylene oxide adduct and derivative thereof, at least one in polyol fatty acid ester and ethylene oxide adduct etc. thereof, described anion surfactant is at least one in calcium dodecyl benzene sulfonate, alkylphenol polyoxyethylene phosphate, phenethyl phenol polyethenoxy ether phosphate etc., as emulsifier 500,601p, 602p etc., in addition, commercially available emulsifier production can also be used.
Wherein, solvent can adopt at least one in water, ethanol, isopropyl alcohol, n-butanol, ethyl acetate, butyl acetate, methyl-sulfoxide, turpentine oil, solvent naphtha, 1-METHYLPYRROLIDONE, dimethyl formamide etc.
Wherein, antifreeze can be glycerine, propane diols, ethylene glycol, sodium chloride, calcium chloride, sorbierite etc.
Wherein, defoamer is silicone defoaming agent etc.
Wherein, dispersant is at least one in lignosulfonates, PAA and propionamide copolymer, alkylnaphthalene sulfonate, polycarboxylic acid salt's condensation product, methyl sodiosul foaliphatate etc.
Wherein, wetting agent can be alkyl sulfate, alkylbenzenesulfonate, alkylnaphthalene sulfonate, aliphatic alcohol polyethenoxy base ether, alkylphenol-polyethenoxy base ether, sorbitan fatty acid ester etc.
Wherein, stabilizing agent can be 2-expoxy propane, butyl glycidyl ether, isopropyl alcohol, n-butanol, polyethylene glycol etc.
The present invention can be prepared into any one formulation of applicable agricultural use containing the composition pesticide of black false hellebore and Ma meter apricot extract by the method for this area routine, for the ease of storing and transport, can make microemulsion, aqua or soluble concentrate.
Further, the soluble concentrate be preferably made up of the component of following weight portion: black false hellebore and Ma meter apricot extract, 10 parts of emulsifier, 602,40 parts of ethanol, wherein, black false hellebore and Ma meter apricot extract are that the crude drug extraction of 50 parts forms.Black false hellebore and Ma meter apricot extract can obtain for black false hellebore and Ma meter Xing mix rear common extraction in proportion; Also first can be extracted separately by black false hellebore and obtain black false hellebore extract, Ma meter Xing extracts separately and obtains horse rice apricot extract, then is mixed by two kinds of extracts.
For the ease of storing and transport, extract can be concentrated, obtain concentrate, and then be prepared into various formulation, cycles of concentration can according to needs of production from Row sum-equal matrix, preferably, black false hellebore is prepared from by the following method with Ma meter apricot extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
Second technical problem that the present invention will solve is to provide the method that preparation contains the composition pesticide of black false hellebore and Ma meter apricot extract.
The preparation method that the present invention contains the composition pesticide of black false hellebore and Ma meter apricot extract is made up of following steps:
A, extraction: take black false hellebore particle and Ma meter Xing particle, mixing, adds solvent by solid-liquid ratio 1:3 ~ 12g/ml, and in 60 ~ 95 DEG C of refluxing extraction, filter, obtain extract, wherein, described solvent is organic solvent or water;
B, preparation: extract is made microemulsion, soluble concentrate or aqua.
Wherein, black false hellebore particle and Ma meter Xing particle can adopt commercially available particle, and conventional method pulverizing black false hellebore rhizome and Ma meter Xing leaf also can be adopted to obtain.In order to improve the effect of extraction, simultaneously cost-saving, black false hellebore particle and Ma meter Xing particle cross 60 ~ 100 mesh sieves, preferred mistake 80 mesh sieve.
Further, in order to cost-saving, simultaneously also in order to improve extraction efficiency, the solid-liquid ratio in a step is preferably 1:4 ~ 10g/ml, is more preferably 1:6g/ml.Namely the black false hellebore of lg and Ma meter Xing particle can add 3 ~ 12ml solvent extraction, preferably add 4 ~ 10ml, more preferably add 6ml.
Conventional organic solvent all can be used for the present invention, and in order to cost-saving, improve dissolution rate, ensure extraction efficiency, organic solvent is preferably methyl alcohol, ethanol, ethyl acetate, acetone, chloroform, is more preferably absolute ethyl alcohol simultaneously.
In order to shorten preparation time, accelerate extraction rate, can stir when adding hot reflux, mixing speed is preferably 80 ~ 100 revs/min.
Further, reflux temperature is preferably 75 ~ 85 DEG C, is more preferably 82 DEG C; Reflow's cycle is twice, and each return time is 2 hours, and merging filtrate, obtains extract.
The application of the composition pesticide that the present invention also provides the present invention to contain black false hellebore and Ma meter apricot extract in control crop pest.The present invention contains the composition pesticide of black false hellebore and Ma meter apricot extract, to homoptera pest as aphid, scale insect, trialeurodes vaporariorum etc.; Acarina pest is as panonychus citri, Tetranychus cinnabarinus etc.; Lepidoptera pest such as cabbage caterpillar, diamond-back moth, prodenia litura, beet snout moth's larva, tea tree tea geometrid etc. have good preventive effect.
In the agricultural production of reality, in order to improve composition pesticide containing black false hellebore and Ma meter apricot extract to the control efficiency of insect pest, the described composition pesticide containing black false hellebore and Ma meter apricot extract is preferably applied to crop pest initial stage of origination; The present invention can use by common method containing the composition pesticide of black false hellebore and Ma meter apricot extract, sprays on crop blade face.Preferably, when using, the present invention is diluted with water to the activity component concentration of crude drug weighing scale as 10 ~ 100ppm containing the composition pesticide of black false hellebore and Ma meter apricot extract, namely the composition pesticide that 1g crude drug is obtained after extracting, when using, be diluted with water to 10kg, then its activity component concentration equals 100ppm.Preferably being diluted to activity component concentration is further that 20 ~ 50ppm sprays in plant blade face, and so that water membrane to appear in blade face, not lower of liquid is advisable; Secondary dispenser is carried out, the preferred continuous administration of a season crop 2 ~ 3 times after being preferable over 5 ~ 7 days.
The present invention is mixed by black false hellebore extract and Ma meter apricot extract containing the composition pesticide of black false hellebore and Ma meter apricot extract, or jointly extracted by black false hellebore and Ma meter Xing and form, its preventive effect is far away higher than the extract of single component, particularly extract common after black false hellebore and Ma meter Xing mixing, owing to having solubilising, hydrotropy effect between black false hellebore and Ma meter Xing, the composition pesticide insecticidal effect made after common extraction is more remarkable.
Compared with prior art, the present invention has the following advantages containing the composition pesticide of black false hellebore and Ma meter apricot extract:
1, the present invention has synergistic function containing the composition pesticide of black false hellebore and Ma meter apricot extract, obvious to the control efficiency of the insects such as Homoptera, Lepidoptera, Acarina.
2, black false hellebore of the present invention and Ma meter Xing mix after jointly extracting or extracting respectively, and the composition pesticide made can improve crop immune system ability while quick-acting tagging, and can significantly improve its vigor.
3, the present invention take Chinese herbal medicine as raw material, thus there is not Pesticide Residue, and insect is difficult to produce resistance; Be used alone compared with chemical source agricultural chemicals, environmentally friendly.
4, when the present invention uses containing the composition pesticide of black false hellebore and Ma meter apricot extract, valid density is low, with low cost.
Embodiment
The present invention contains the composition pesticide of black false hellebore and Ma meter apricot extract, and its active component is extracted by the crude drug of following weight portion and forms: black false hellebore 1 part, Ma meter Xing 0.5 ~ 6 part.Wherein, active component is preferably extracted by the crude drug of following weight portion and forms: black false hellebore 1 part, Ma meter Xing 1 ~ 4 part; Be preferably again and formed by the crude drug extraction of following weight portion: black false hellebore 1 part, Ma meter Xing 1 ~ 3 part; Be more preferably and formed by the crude drug extraction of following weight portion: black false hellebore 1 part, Ma meter Xing 2 parts.
Wherein, the black false hellebore that the present invention adopts and Ma meter Xing are Chinese herbal medicine, contain the activity of the composition pesticide of black false hellebore and Ma meter apricot extract in order to improve the present invention, the black false hellebore rhizome that black false hellebore is preferably dry, the Ma meter Xing leaf that Ma meter Xing is preferably dry.
The present invention contains the composition pesticide of black false hellebore and Ma meter apricot extract, and after its active component can be mixed by crude drug black false hellebore and Ma meter Xing, common extraction forms; Also first can be extracted separately by black false hellebore and obtain black false hellebore extract, Ma meter Xing extracts separately and obtains horse rice apricot extract, then is mixed by two kinds of extracts.In order to improve the insecticidal properties of composition pesticide of the present invention, after its active component is preferably and is mixed by black false hellebore and Ma meter Xing, common extraction forms.
Further, in order to improve the stability of the composition pesticide containing black false hellebore and Ma meter apricot extract, the present invention contains in the composition pesticide of black false hellebore and Ma meter apricot extract and also comprises auxiliary agent, described auxiliary agent can adopt the usual auxiliaries in agricultural chemicals, as at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
Wherein, emulsifier can adopt at least one in non-ionic surface active agent and anion surfactant, described non-ionic surface active agent can be dibenzyl xenyl APEO, styryl phenyl APEO, styryl phenyl APEO, phenethyl phenyl polyoxyethylene one polyethenoxy ether, OPEO, alkylphenol polyoxyethylene sulfosuccinate, aliphatic acid polyethenoxy ether, fatty alcohol-polyoxyethylene ether, aliphatic amine polyoxyethylene ether, castor oil ethylene oxide adduct and derivative thereof, at least one in polyol fatty acid ester and ethylene oxide adduct etc. thereof, described anion surfactant is at least one in calcium dodecyl benzene sulfonate, alkylphenol polyoxyethylene phosphate, phenethyl phenol polyethenoxy ether phosphate etc., as emulsifier 500,601p, 602p etc., in addition, commercially available emulsifier production can also be used.
Wherein, solvent can adopt at least one in water, ethanol, isopropyl alcohol, n-butanol, ethyl acetate, butyl acetate, methyl-sulfoxide, turpentine oil, solvent naphtha, 1-METHYLPYRROLIDONE, dimethyl formamide etc.
Wherein, antifreeze can be glycerine, propane diols, ethylene glycol, sodium chloride, calcium chloride, sorbierite etc.
Wherein, defoamer is silicone defoaming agent etc.
Wherein, dispersant is at least one in lignosulfonates, PAA and propionamide copolymer, alkylnaphthalene sulfonate, polycarboxylic acid salt's condensation product, methyl sodiosul foaliphatate etc.
Wherein, wetting agent can be alkyl sulfate, alkylbenzenesulfonate, alkylnaphthalene sulfonate, aliphatic alcohol polyethenoxy base ether, alkylphenol-polyethenoxy base ether, sorbitan fatty acid ester etc.
Wherein, stabilizing agent can be 2-expoxy propane, butyl glycidyl ether, isopropyl alcohol, n-butanol, polyethylene glycol etc.
The present invention can be prepared into any one formulation of applicable agricultural use containing the composition pesticide of black false hellebore and Ma meter apricot extract by the method for this area routine, for the ease of storing and transport, can make microemulsion, aqua or soluble concentrate.
Further, the soluble concentrate be preferably made up of the component of following weight portion: black false hellebore and Ma meter apricot extract, 10 parts of emulsifier, 602,40 parts of ethanol, wherein, black false hellebore and Ma meter apricot extract are that the crude drug extraction of 50 parts forms.Black false hellebore and Ma meter apricot extract can obtain for black false hellebore and Ma meter Xing mix rear common extraction in proportion; Also first can be extracted separately by black false hellebore and obtain black false hellebore extract, Ma meter Xing extracts separately and obtains horse rice apricot extract, then is mixed by two kinds of extracts.
For the ease of storing and transport, extract can be concentrated, obtain concentrate, and then be prepared into various formulation, cycles of concentration can according to needs of production from Row sum-equal matrix, preferably, black false hellebore is prepared from by the following method with Ma meter apricot extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
The preparation method that the present invention contains the composition pesticide of black false hellebore and Ma meter apricot extract is made up of following steps:
A, extraction: take black false hellebore particle and Ma meter Xing particle, mixing, adds solvent by solid-liquid ratio 1:3 ~ 12g/ml, and in 60 ~ 95 DEG C of refluxing extraction, filter, obtain extract, wherein, described solvent is organic solvent or water;
B, preparation: extract is made microemulsion, soluble concentrate or aqua.
Wherein, black false hellebore particle and Ma meter Xing particle can adopt commercially available particle, and conventional method pulverizing black false hellebore rhizome and Ma meter Xing leaf also can be adopted to obtain.In order to improve the effect of extraction, simultaneously cost-saving, black false hellebore particle and Ma meter Xing particle cross 60 ~ 100 mesh sieves, preferred mistake 80 mesh sieve.
Further, in order to cost-saving, simultaneously also in order to improve extraction efficiency, the solid-liquid ratio in a step is preferably 1:4 ~ 10g/ml, is more preferably 1:6g/ml.Namely the black false hellebore of lg and Ma meter Xing particle can add 3 ~ 12ml solvent extraction, preferably add 4 ~ 10ml, more preferably add 6ml.
Conventional organic solvent all can be used for the present invention, and in order to cost-saving, improve dissolution rate, ensure extraction efficiency, organic solvent is preferably methyl alcohol, ethanol, ethyl acetate, acetone, chloroform, is more preferably absolute ethyl alcohol simultaneously.
In order to shorten preparation time, accelerate extraction rate, can stir when adding hot reflux, mixing speed is preferably 80 ~ 100 revs/min.
Further, reflux temperature is preferably 75 ~ 85 DEG C, is more preferably 82 DEG C; Reflow's cycle is twice, and each return time is 2 hours, and merging filtrate, obtains extract.
The present invention contains the composition pesticide of black false hellebore and Ma meter apricot extract, to homoptera pest as aphid, scale insect, trialeurodes vaporariorum etc.; Acarina pest is as panonychus citri, Tetranychus cinnabarinus etc.; Lepidoptera pest such as cabbage caterpillar, diamond-back moth, prodenia litura, beet snout moth's larva, tea tree tea geometrid etc. have good preventive effect.
In the agricultural production of reality, in order to improve composition pesticide containing black false hellebore and Ma meter apricot extract to the control efficiency of insect pest, the described composition pesticide containing black false hellebore and Ma meter apricot extract is preferably applied to crop pest initial stage of origination; The present invention can use by common method containing the composition pesticide of black false hellebore and Ma meter apricot extract, sprays on crop blade face.Preferably, when using, the present invention is diluted with water to the activity component concentration of crude drug weighing scale as 10 ~ 100ppm containing the composition pesticide of black false hellebore and Ma meter apricot extract, namely the composition pesticide that 1g crude drug is obtained after extracting, when using, be diluted with water to 10kg, then its activity component concentration equals 100ppm.Preferably being diluted to activity component concentration is further that 20 ~ 50ppm sprays in plant blade face, and so that water membrane to appear in blade face, not lower of liquid is advisable; Secondary dispenser is carried out, the preferred continuous administration of a season crop 2 ~ 3 times after being preferable over 5 ~ 7 days.
The present invention is mixed by black false hellebore extract and Ma meter apricot extract containing the composition pesticide of black false hellebore and Ma meter apricot extract, or jointly extracted by black false hellebore and Ma meter Xing and form, its preventive effect is far away higher than the extract of single component, particularly extract common after black false hellebore and Ma meter Xing mixing, owing to having solubilising, hydrotropy effect between black false hellebore and Ma meter Xing, the composition pesticide insecticidal effect made after common extraction is more remarkable.
Below in conjunction with embodiment, the specific embodiment of the present invention is further described, does not therefore limit the present invention among described scope of embodiments.
Embodiment 1 contains the preparation of the composition pesticide of black false hellebore and Ma meter apricot extract
A, extraction: take dry black false hellebore rhizome and Ma meter Xing leaf, mixing, pulverizes, and crosses 80 mesh sieves, adds extraction solution, refluxing extraction twice, filters, merging filtrate, concentrated, makes extract weight equal the weight of crude drug, obtains extract lm-01 ~ lm-06; Wherein, each parameter is in table 1;
B, preparation: in extract lm-01 ~ lm-06, add auxiliary agent in table 2, stir, must contain the composition pesticide LM-01 ~ LM-06 of black false hellebore and Ma meter apricot extract.
The preparation technology parameter of table 1 black false hellebore and Ma meter apricot extract
Continued 1
| lm-05 | 1 | 4 | 1:8 | Methyl alcohol | 75 | 2 |
| lm-06 | 1 | 6 | 1:4 | Chloroform | 60 | 1 |
Wherein, black false hellebore rhizome and Ma meter Xing leaf are all purchased from Anguo City Zhong Tian prepared slices of Chinese crude drugs Co., Ltd.
Table 2 contains the formula of the composition pesticide of black false hellebore and Ma meter apricot extract
Embodiment 2 contains the preparation of the composition pesticide of black false hellebore and Ma meter apricot extract
A, extraction: take dry black false hellebore rhizome, pulverize, and crosses 80 mesh sieves, add extraction solution, refluxing extraction twice, filters, merging filtrate, and concentrated, make extract weight equal the weight of crude drug, obtain extract d1-01 ~ d1-03, wherein, each parameter is in table 3;
Take dry Ma meter Xing leaf, pulverize, cross 80 mesh sieves, add extraction solution, refluxing extraction twice, filter, merging filtrate, concentrated, make extract weight equal the weight of crude drug, obtain extract d2-01 ~ d2-03, wherein, each parameter is in table 4;
B, preparation: take black false hellebore extract, Ma meter Xing extract and auxiliary agent, stirring and evenly mixing, obtain the composition pesticide LM-07 ~ LM-12 containing black false hellebore and Ma meter apricot extract, wherein, each parameter is in table 5.
Table 3
Table 4
Table 5
Test example 1 the present invention contains the efficiency test of the composition pesticide citrus red mite of black false hellebore and Ma meter apricot extract
For clearly putting into field efficacy situation containing the composition pesticide of black false hellebore and Ma meter apricot extract, inventor has carried out field control effectiveness test to this medicament.
1, test material
1.1 for studying thing and object: citrus red mite
1.2 reagent agent
Product in embodiment: LM-01 ~ LM-12;
Black false hellebore single dose (D1), Ma meter Xing single dose (D2);
Chemical reagent contrast (CK-01): 1.8% abamectin emulsifiable concentrate, purchased from Longdeng Chemical Co Ltd, Jiangsu;
Clear water contrast (CK-02).
Wherein, the preparation method of black false hellebore single dose is: the d1-03 getting 50g, adds 10g emulsifier 602,40g ethanol, stirring and evenly mixing, obtained black false hellebore single dose.
The preparation method of Ma meter Xing single dose is: the d2-03 getting 50g, adds 10g emulsifier 602,40g ethanol, stirring and evenly mixing, obtained Ma meter Xing single dose.
2, experimental scheme and method
This test 12 medicaments, 2 single dose contrasts, 1 contrast medicament, 1 blank, repeats for three times, Gong48Ge community.Minizone and surrounding all leave isolation strip, plot area 20m
2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the two spotted spider mite quantity statistics of blade before the test and listing mark.Within 5 days after dispenser, investigate and record the two spotted spider mite number alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
3, result of the test
Each group is repeated meter of averaging, and calculate Revision insect recluced rate and preventive effect, it the results are shown in Table 6.
Table 6
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| LM-01 | 311 | 102 | 67.20 | 71.79 |
| LM-02 | 352 | 101 | 71.31 | 75.32 |
| LM-03 | 324 | 85 | 73.77 | 77.43 |
| LM-04 | 364 | 102 | 71.98 | 75.90 |
| LM-05 | 347 | 102 | 70.61 | 74.71 |
| LM-06 | 364 | 117 | 67.86 | 72.35 |
| LM-07 | 328 | 147 | 55.18 | 61.45 |
| LM-08 | 391 | 152 | 61.13 | 66.56 |
| LM-09 | 348 | 127 | 63.51 | 68.61 |
| LM-10 | 344 | 132 | 61.63 | 66.99 |
Continued 6
| LM-11 | 320 | 131 | 59.06 | 64.79 |
| LM-12 | 355 | 154 | 56.62 | 62.68 |
| D1 | 349 | 292 | 16.33 | 28.03 |
| D2 | 347 | 311 | 10.37 | 22.91 |
| CK-01 | 351 | 84 | 76.07 | 79.41 |
| CK-02 | 363 | 422 | -16.25 | —— |
As can be seen from Table 6, product of the present invention has good preventive effect to two spotted spider mite, and better, visible than the control efficiency of single black false hellebore extract and Ma meter apricot extract, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.In addition, have no during dispenser and have poisoning to show to crop.
Test example 2 the present invention contains the efficiency test of the composition pesticide eggplant aphid of black false hellebore and Ma meter apricot extract
Product LM-01 ~ LM-12 of the present invention is used for preventing and treating eggplant aphid.This test 12 medicaments, 2 single doses, 1 blank, repeats for three times, Gong45Ge community.Minizone and surrounding all leave isolation strip, plot area 10m
2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the Aphed population statistics alive also listing mark of blade before the test.Within 5 days after dispenser, investigate and record the aphid number alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group is repeated meter of averaging, and calculate Revision insect recluced rate and preventive effect, it the results are shown in Table 7.
Table 7
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| LM-01 | 344 | 75 | 78.20 | 81.43 |
| LM-02 | 356 | 51 | 85.67 | 87.80 |
| LM-03 | 342 | 42 | 87.72 | 89.54 |
Continued 7
| LM-04 | 367 | 55 | 85.01 | 87.24 |
| LM-05 | 346 | 67 | 80.64 | 83.51 |
| LM-06 | 389 | 81 | 79.18 | 82.27 |
| LM-07 | 375 | 113 | 69.87 | 74.34 |
| LM-08 | 316 | 75 | 76.27 | 79.79 |
| LM-09 | 327 | 72 | 77.98 | 81.25 |
| LM-10 | 338 | 83 | 75.44 | 79.09 |
| LM-11 | 385 | 100 | 74.03 | 77.88 |
| LM-12 | 364 | 99 | 72.80 | 76.84 |
| D1 | 385 | 276 | 28.31 | 38.94 |
| D2 | 311 | 263 | 15.43 | 27.98 |
| CK-02 | 379 | 445 | -17.41 | —— |
As can be seen from Table 7, product of the present invention has good preventive effect to aphid, and better, visible than the control efficiency of single black false hellebore extract and Ma meter apricot extract, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.In addition, have no during dispenser and have poisoning to show to crop.
Test example 3 the present invention contains the efficiency test of the composition pesticide oranges and tangerines cottonycushion scale of black false hellebore and Ma meter apricot extract
Product LM-01 ~ LM-12 of the present invention is used for preventing and treating oranges and tangerines cottonycushion scale.This test 12 medicaments, 2 single doses, 1 blank, repeats for three times, Gong45Ge community.Minizone and surrounding all leave isolation strip, plot area 20m
2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the cottonycushion scale quantity statistics alive of blade before the test and listing mark.Within 5 days after dispenser, investigate and record the cottonycushion scale number alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group is repeated meter of averaging, and calculate Revision insect recluced rate and preventive effect, its result is as shown in table 8.
Table 8
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| LM-01 | 439 | 149 | 66.06 | 71.82 |
| LM-02 | 423 | 116 | 72.58 | 77.23 |
| LM-03 | 457 | 110 | 75.93 | 80.02 |
| LM-04 | 453 | 120 | 73.51 | 78.01 |
| LM-05 | 449 | 134 | 70.16 | 75.22 |
| LM-06 | 477 | 151 | 68.34 | 73.72 |
| LM-07 | 469 | 162 | 65.46 | 71.33 |
| LM-08 | 427 | 197 | 53.86 | 61.70 |
| LM-09 | 436 | 161 | 63.07 | 69.35 |
| LM-10 | 452 | 179 | 60.40 | 67.12 |
| LM-11 | 474 | 200 | 57.81 | 64.97 |
| LM-12 | 418 | 188 | 55.02 | 62.66 |
| D1 | 430 | 374 | 13.02 | 27.80 |
| D2 | 431 | 397 | 7.89 | 23.53 |
| CK-02 | 479 | 577 | -20.46 | —— |
As can be seen from Table 8, product of the present invention has good preventive effect to cottonycushion scale, and better, visible than the control efficiency of single black false hellebore extract and Ma meter apricot extract, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.In addition, have no during dispenser and have poisoning to show to crop.
Test example 4 the present invention contains the efficiency test of the composition pesticide a variety of Chinese cabbage diamond-back moth of black false hellebore and Ma meter apricot extract
Product LM-01 ~ LM-12 of the present invention is used for preventing and treating a variety of Chinese cabbage diamond-back moth.This test 12 medicaments, 2 single doses, 1 blank, repeats for three times, Gong45Ge community.Minizone and surrounding all leave isolation strip, plot area 10m
2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the diamond-back moth quantity statistics alive of blade before the test and listing mark.Within 5 days after dispenser, investigate and record the diamond-back moth number alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group is repeated meter of averaging, and calculate Revision insect recluced rate and preventive effect, its result is as shown in table 9.
Table 9
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| LM-01 | 512 | 107 | 79.10 | 81.93 |
| LM-02 | 548 | 75 | 86.31 | 88.16 |
| LM-03 | 529 | 55 | 89.60 | 91.01 |
| LM-04 | 534 | 68 | 87.27 | 88.99 |
| LM-05 | 543 | 99 | 81.77 | 84.23 |
| LM-06 | 541 | 102 | 81.15 | 83.70 |
| LM-07 | 553 | 171 | 69.08 | 73.26 |
| LM-08 | 559 | 137 | 75.49 | 78.81 |
| LM-09 | 583 | 132 | 77.36 | 80.42 |
| LM-10 | 592 | 149 | 74.83 | 78.23 |
| LM-11 | 527 | 141 | 73.24 | 76.86 |
| LM-12 | 552 | 155 | 71.92 | 75.72 |
| D1 | 595 | 388 | 34.79 | 43.61 |
| D2 | 538 | 375 | 30.30 | 39.72 |
| CK-02 | 518 | 599 | -15.64 | —— |
As can be seen from Table 9, product of the present invention has good preventive effect to diamond-back moth, and better, visible than the control efficiency of single black false hellebore extract and Ma meter apricot extract, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.In addition, have no during dispenser and have poisoning to show to crop.
Test example 5 the present invention contains the efficiency test of the composition pesticide cotton cotton bollworm of black false hellebore and Ma meter apricot extract
Product LM-01 ~ LM-12 of the present invention is used for preventing and treating cotton cotton bollworm.This test 12 medicaments, 2 single doses, 1 blank, repeats for three times, Gong45Ge community.Minizone and surrounding all leave isolation strip, plot area 10m
2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the cotton bollworm quantity statistics alive of blade before the test and listing mark.Within 5 days after dispenser, investigate and record the cotton boll borer population alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group is repeated meter of averaging, and calculate Revision insect recluced rate and preventive effect, its result is as shown in table 10.
Table 10
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| LM-01 | 411 | 97 | 76.40 | 80.92 |
| LM-02 | 423 | 73 | 82.74 | 86.04 |
| LM-03 | 467 | 74 | 84.15 | 87.19 |
| LM-04 | 485 | 84 | 82.68 | 85.99 |
| LM-05 | 436 | 85 | 80.50 | 84.24 |
| LM-06 | 448 | 98 | 78.13 | 82.31 |
| LM-07 | 459 | 167 | 63.62 | 70.58 |
| LM-08 | 462 | 133 | 71.21 | 76.72 |
| LM-09 | 494 | 132 | 73.28 | 78.39 |
| LM-10 | 447 | 131 | 70.69 | 76.30 |
| LM-11 | 463 | 144 | 68.90 | 74.85 |
| LM-12 | 472 | 158 | 66.53 | 72.93 |
| D1 | 468 | 407 | 13.03 | 29.68 |
| D2 | 478 | 441 | 7.74 | 25.40 |
| CK-02 | 431 | 533 | -23.67 | —— |
As can be seen from Table 10, product of the present invention has good preventive effect to cotton bollworm, and better, visible than the control efficiency of single black false hellebore extract and Ma meter apricot extract, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.In addition, have no during dispenser and have poisoning to show to crop.
Claims (25)
1. contain the composition pesticide of black false hellebore and Ma meter apricot extract, it is characterized in that, its active component is extracted by the crude drug of following weight portion and forms: black false hellebore 1 part, Ma meter Xing 0.5 ~ 6 part.
2. the composition pesticide containing black false hellebore and Ma meter apricot extract according to claim 1, is characterized in that, its active component is extracted by the crude drug of following weight portion and forms: black false hellebore 1 part, Ma meter Xing 1 ~ 4 part.
3. the composition pesticide containing black false hellebore and Ma meter apricot extract according to claim 2, is characterized in that, its active component is extracted by the crude drug of following weight portion and forms: black false hellebore 1 part, Ma meter Xing 1 ~ 3 part.
4. the composition pesticide containing black false hellebore and Ma meter apricot extract according to claim 3, is characterized in that, its active component is extracted by the crude drug of following weight portion and forms: black false hellebore 1 part, Ma meter Xing 2 parts.
5. the composition pesticide containing black false hellebore and Ma meter apricot extract according to any one of Claims 1 to 4, is characterized in that: after its active component is mixed by black false hellebore and Ma meter Xing, common extraction forms; Or extract by black false hellebore the black false hellebore extract, the Ma meter Xing that obtain and extract the horse rice apricot extract obtained and mix.
6. the composition pesticide containing black false hellebore and Ma meter apricot extract according to any one of Claims 1 to 4, is characterized in that: the described composition pesticide containing black false hellebore and Ma meter apricot extract is microemulsion, aqua or soluble concentrate.
7. the composition pesticide containing black false hellebore and Ma meter apricot extract according to claim 5, is characterized in that: the described composition pesticide containing black false hellebore and Ma meter apricot extract is microemulsion, aqua or soluble concentrate.
8. the composition pesticide containing black false hellebore and Ma meter apricot extract according to any one of Claims 1 to 4, it is characterized in that: described containing in the composition pesticide of black false hellebore and Ma meter apricot extract also comprises auxiliary agent, and described auxiliary agent is at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
9. the composition pesticide containing black false hellebore and Ma meter apricot extract according to claim 5, it is characterized in that: described containing in the composition pesticide of black false hellebore and Ma meter apricot extract also comprises auxiliary agent, and described auxiliary agent is at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
10. the composition pesticide containing black false hellebore and Ma meter apricot extract according to claim 6, it is characterized in that: described containing in the composition pesticide of black false hellebore and Ma meter apricot extract also comprises auxiliary agent, and described auxiliary agent is at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
11. composition pesticides containing black false hellebore and Ma meter apricot extract according to claim 7, it is characterized in that: described containing in the composition pesticide of black false hellebore and Ma meter apricot extract also comprises auxiliary agent, and described auxiliary agent is at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
12. composition pesticides containing black false hellebore and Ma meter apricot extract according to claim 6, it is characterized in that: described soluble concentrate is made up of the component of following weight portion: black false hellebore and Ma meter apricot extract, 10 parts of emulsifier 602,40 parts of ethanol, wherein, black false hellebore and Ma meter apricot extract be 50 parts crude drug extract form.
13. composition pesticides containing black false hellebore and Ma meter apricot extract according to claim 7, it is characterized in that: described soluble concentrate is made up of the component of following weight portion: black false hellebore and Ma meter apricot extract, 10 parts of emulsifier 602,40 parts of ethanol, wherein, black false hellebore and Ma meter apricot extract be 50 parts crude drug extract form.
14. composition pesticides containing black false hellebore and Ma meter apricot extract according to claim 10, it is characterized in that: described soluble concentrate is made up of the component of following weight portion: black false hellebore and Ma meter apricot extract, 10 parts of emulsifier 602,40 parts of ethanol, wherein, black false hellebore and Ma meter apricot extract be 50 parts crude drug extract form.
15. composition pesticides containing black false hellebore and Ma meter apricot extract according to claim 11, it is characterized in that: described soluble concentrate is made up of the component of following weight portion: black false hellebore and Ma meter apricot extract, 10 parts of emulsifier 602,40 parts of ethanol, wherein, black false hellebore and Ma meter apricot extract be 50 parts crude drug extract form.
16. composition pesticides containing black false hellebore and Ma meter apricot extract according to claim 12, is characterized in that: black false hellebore is prepared from by the following method with Ma meter apricot extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
17. composition pesticides containing black false hellebore and Ma meter apricot extract according to claim 13, is characterized in that: black false hellebore is prepared from by the following method with Ma meter apricot extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
18. composition pesticides containing black false hellebore and Ma meter apricot extract according to claim 14, is characterized in that: black false hellebore is prepared from by the following method with Ma meter apricot extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
19. composition pesticides containing black false hellebore and Ma meter apricot extract according to claim 15, is characterized in that: black false hellebore is prepared from by the following method with Ma meter apricot extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
The method containing the composition pesticide of black false hellebore and Ma meter apricot extract described in 20. any one of preparation claim 1 ~ 19, is characterized in that being made up of following steps:
A, extraction: take black false hellebore particle and Ma meter Xing particle, mixing, adds solvent by solid-liquid ratio 1:3 ~ 12g/ml, and in 60 ~ 95 DEG C of refluxing extraction, filter, obtain extract, wherein, described solvent is organic solvent or water;
B, preparation: extract is made microemulsion, soluble concentrate or aqua.
21. preparations according to claim 20 contain the method for the composition pesticide of black false hellebore and Ma meter apricot extract, and it is characterized in that: in described a step, black false hellebore particle and Ma meter Xing particle cross 60 ~ 100 mesh sieves; Solid-liquid ratio is 1:4 ~ 10g/ml; Organic solvent is methyl alcohol, ethanol, ethyl acetate, acetone, chloroform; Reflux temperature is 75 ~ 85 DEG C; Reflow's cycle is twice, and merging filtrate, obtains extract.
22. preparations according to claim 21 contain the method for the composition pesticide of black false hellebore and Ma meter apricot extract, and it is characterized in that: in described a step, black false hellebore particle and Ma meter Xing particle cross 80 mesh sieves; Solid-liquid ratio is 1:6g/ml; Organic solvent is absolute ethyl alcohol; Reflux temperature is 82 DEG C.
The composition pesticide containing black false hellebore and Ma meter apricot extract described in 23. any one of claim 1 ~ 19 is preventing and treating the application in crop pest.
24. application of composition pesticide in control crop pest containing black false hellebore and Ma meter apricot extract according to claim 23, is characterized in that: described insect pest is homoptera pest, Lepidoptera, acarina pest; During application, be diluted with water to the activity component concentration of crude drug weighing scale as 10 ~ 100ppm.
25. application of composition pesticide in control crop pest containing black false hellebore and Ma meter apricot extract according to claim 24, is characterized in that: be diluted with water to the activity component concentration of crude drug weighing scale as 20 ~ 50ppm.
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| CN100999341A (en) * | 2006-01-09 | 2007-07-18 | 吴兆流 | Solar energy seawater desalination machine set |
| CN101015303A (en) * | 2006-12-25 | 2007-08-15 | 洪云飞 | Plant mosquito-fly pesticide |
| KR20120004832A (en) * | 2010-07-07 | 2012-01-13 | 박지웅 | Spray type insecticide, manufacturing method of the spray type insecticide and insecticide sprayer |
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| CN100999341A (en) * | 2006-01-09 | 2007-07-18 | 吴兆流 | Solar energy seawater desalination machine set |
| CN101015303A (en) * | 2006-12-25 | 2007-08-15 | 洪云飞 | Plant mosquito-fly pesticide |
| KR20120004832A (en) * | 2010-07-07 | 2012-01-13 | 박지웅 | Spray type insecticide, manufacturing method of the spray type insecticide and insecticide sprayer |
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