CN105284914A - Pesticide composition containing cynanchum komarovii and sophora alopecuroide extract as well as preparation method and application thereof - Google Patents
Pesticide composition containing cynanchum komarovii and sophora alopecuroide extract as well as preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to a pesticide composition containing cynanchum komarovii and sophora alopecuroide extract as well as a preparation method and application thereof and belongs to the field of pesticides. Aiming at solving the technical problems, the invention provides the pesticide composition containing the cynanchum komarovii and sophora alopecuroide extract as well as the preparation method and the application thereof. According to the pesticide composition containing the cynanchum komarovii and sophora alopecuroide extract, active ingredients are obtained by extracting following raw medicinal materials in parts by weight: 1 part of cynanchum komarovii and 0.5-6 parts of sophora alopecuroide. The pesticide composition containing the cynanchum komarovii and sophora alopecuroide extract can be used for killing homoptera and hemiptera insects very well, such as scale insects, aphids, trialeurodes vaporariorum and lugus lucorum, and has very active meanings on agricultural production.
Description
Technical field
The present invention relates to the composition pesticide containing Cynanchum Komarovii and sophora alopecuroides extract and preparation method and application, belong to pesticide field.
Background technology
Sophora alopecuroide is cassia leguminous plant, another name matrine, foxtail-like sophora root, herba sophorae alopecuroide.Sophora alopecuroide is perennial herb, and complete stool is gathered canescence volt kiginu shape pubescence.Owing to being rich in alkaloid in its root, stem, leaf and fruit, mainly contain matrine, oxymatrine, sophocarpine, sophoramine and eulexine, thus Sophora alopecuroide has very high medical value.
Cynanchum Komarovii, Asclepiadaceae, Cynanchum perennial vertical herbage, coring shape; Stem is had single-row pubescence and young tender part to have outside microtriche, remaining all without hair, single stem or slightly branch.Leaf to life, papery, ovum shape lanceolar, top is gradually sharp, the wide wedge shape of base portion; At edge anastomose, sometimes there is limit hair; Top body of gland in groups.
When prior art to there is not yet about Cynanchum Komarovii and sophora alopecuroides extract as Pesticide use, there is the relevant report of synergistic function.
Summary of the invention
The technical problem to be solved in the present invention is to provide composition pesticide containing Cynanchum Komarovii and sophora alopecuroides extract and preparation method and application.
The present invention contains the composition pesticide of Cynanchum Komarovii and sophora alopecuroides extract, and its active component is extracted by the crude drug of following weight portion and forms: Cynanchum Komarovii 1 part, Sophora alopecuroide 0.5 ~ 6 part.Wherein, active component is preferably extracted by the crude drug of following weight portion and forms: Cynanchum Komarovii 1 part, Sophora alopecuroide 0.5 ~ 5 part.
Wherein, the Cynanchum Komarovii that the present invention adopts and Sophora alopecuroide are Chinese herbal medicine, the activity of the composition pesticide of Cynanchum Komarovii and sophora alopecuroides extract is contained, the acrial parts such as the Cynanchum Komarovii cauline leaf that Cynanchum Komarovii is preferably dry, the Sophora alopecuroide stem stalk that Sophora alopecuroide is preferably dry in order to improve the present invention.
The present invention contains the composition pesticide of Cynanchum Komarovii and sophora alopecuroides extract, and after its active component can be mixed by crude drug Cynanchum Komarovii and Sophora alopecuroide, common extraction forms; Also first can be extracted separately by Cynanchum Komarovii and obtain Cynanchum Komarovii extract, Sophora alopecuroide extracts separately and obtains sophora alopecuroides extract, then is mixed by two kinds of extracts.In order to improve the insecticidal properties of composition pesticide of the present invention, after its active component is preferably and is mixed by Cynanchum Komarovii and Sophora alopecuroide, common extraction forms.
Further, in order to improve the stability of the composition pesticide containing Cynanchum Komarovii and sophora alopecuroides extract, of the present invention containing in the composition pesticide of Cynanchum Komarovii and sophora alopecuroides extract also comprises auxiliary agent, described auxiliary agent can adopt the usual auxiliaries in agricultural chemicals, as at least one in emulsifier, antifreeze, solvent, defoamer, stabilizing agent.
Wherein, emulsifier can adopt at least one in non-ionic surface active agent or anion surfactant, described non-ionic surface active agent can be dibenzyl xenyl APEO, styryl phenyl APEO, styryl phenyl APEO, phenethyl phenyl polyoxyethylene one polyethenoxy ether, OPEO, alkylphenol polyoxyethylene sulfosuccinate, aliphatic acid polyethenoxy ether, fatty alcohol-polyoxyethylene ether, aliphatic amine polyoxyethylene ether, castor oil ethylene oxide adduct and derivative thereof, at least one in polyol fatty acid ester and ethylene oxide adduct etc. thereof, described anion surfactant is at least one in calcium dodecyl benzene sulfonate, alkylphenol polyoxyethylene phosphate, phenethyl phenol polyethenoxy ether phosphate etc., as emulsifier 500,601p, 602p, op-10 etc., in addition, commercially available emulsifier production can also be used.
Wherein, solvent can adopt at least one in water, ethanol, isopropyl alcohol, n-butanol, ethyl acetate, butyl acetate, methyl-sulfoxide, turpentine oil, solvent naphtha, 1-METHYLPYRROLIDONE, dimethyl formamide etc.
Wherein, antifreeze can be glycerine, propane diols, ethylene glycol, sodium chloride, calcium chloride, sorbierite etc.
Wherein, defoamer is silicone defoaming agent etc.
Wherein, stabilizing agent can be 2-expoxy propane, butyl glycidyl ether, isopropyl alcohol, n-butanol, polyethylene glycol etc.
The composition pesticide containing Cynanchum Komarovii and sophora alopecuroides extract of the present invention can be prepared into any one formulation of applicable agricultural use by the method for this area routine, for the ease of storing and transport, can make microemulsion, soluble concentrate or aqua.
Further, the microemulsion be preferably made up of the component of following weight portion: described microemulsion is made up of the component of following weight portion: Cynanchum Komarovii and sophora alopecuroides extract, 20 parts of absolute ethyl alcohols, 15 parts of fatty alcohol-polyoxyethylene ether, 5 parts of emulsifier alkyl benzene calcium sulfonates, 5 parts of ethylene glycol, 25 parts of water; Wherein, Cynanchum Komarovii and sophora alopecuroides extract be 30 parts crude drug extract form; Or:
The described composition pesticide containing Cynanchum Komarovii and sophora alopecuroides extract is aqua, and described aqua is made up of the component of following weight portion: Cynanchum Komarovii and sophora alopecuroides extract, 5 parts of absolute ethyl alcohols, 5 parts of emulsifier, 1601,5 parts of ethylene glycol, 35 parts of water; Wherein, Cynanchum Komarovii and sophora alopecuroides extract be 50 parts crude drug extract form; Or:
The described composition pesticide containing Cynanchum Komarovii and sophora alopecuroides extract is microemulsion, described microemulsion is made up of the component of following weight portion: Cynanchum Komarovii and sophora alopecuroides extract, 10 parts of absolute ethyl alcohols, 15 parts of emulsifier op-10,3 parts of emulsifier calcium dodecyl benzene sulfonates, 5 parts of ethylene glycol, 5 parts of dimethyl sulfoxide (DMSO)s, 12 parts of water; Wherein, Cynanchum Komarovii and sophora alopecuroides extract be 50 parts crude drug extract form.
For the ease of storing and transport, extract can be concentrated, obtaining concentrate, and then being prepared into various formulation, cycles of concentration can according to needs of production from Row sum-equal matrix, preferably, in mass ratio, and concentrate: crude drug=1:1.
Further, the invention also discloses the preparation method that the present invention contains the composition pesticide of Cynanchum Komarovii and sophora alopecuroides extract, it is made up of following steps:
A, extraction: take Cynanchum Komarovii particle and Sophora alopecuroide particle, mixing, adds solvent by solid-liquid ratio 1:3 ~ 12g/ml, and in 50 ~ 100 DEG C of refluxing extraction 2 ~ 3 hours, filter, obtain extract, wherein, described solvent was organic solvent or water;
B, preparation: extract is made microemulsion, soluble concentrate or aqua.
Wherein, Cynanchum Komarovii particle and Sophora alopecuroide particle can adopt commercially available particle, and the acrial parts such as conventional method pulverizing Cynanchum Komarovii cauline leaf and Sophora alopecuroide stem stalk also can be adopted to obtain.In order to improve the effect of extraction, simultaneously cost-saving, Cynanchum Komarovii particle and Sophora alopecuroide particle cross 60 ~ 100 mesh sieves, preferred mistake 80 mesh sieve.
Further, in order to cost-saving, simultaneously also in order to improve extraction efficiency, the solid-liquid ratio in a step is preferably 1:4 ~ 8g/ml.Namely the Cynanchum Komarovii of lg and Sophora alopecuroide particle can add 4 ~ 8ml.
Conventional organic solvent all can be used for the present invention, and in order to cost-saving, improve dissolution rate, ensure extraction efficiency, organic solvent is preferably methyl alcohol, ethanol, ethyl acetate, acetone, chloroform simultaneously.
In order to shorten preparation time, accelerate extraction rate, can stir when adding hot reflux, mixing speed is preferably 80 ~ 100 revs/min.
Further, reflux temperature is preferably 75 ~ 85 DEG C; Reflow's cycle is twice, and each return time is 2 hours, and merging filtrate, obtains extract.
The present invention also provides the composition pesticide containing Cynanchum Komarovii and sophora alopecuroides extract of the present invention preventing and treating the application in crop pest.The composition pesticide containing Cynanchum Komarovii and sophora alopecuroides extract of the present invention, to homoptera pest as aphid, coccid, trialeurodes vaporariorum etc.; Hemipteran pest such as mirid pentatomid etc. has good preventive effect.
In the agricultural production of reality, in order to improve composition pesticide containing Cynanchum Komarovii and sophora alopecuroides extract to the control efficiency of insect pest, the described composition pesticide containing Cynanchum Komarovii and sophora alopecuroides extract is preferably applied to crop pest initial stage of origination; Composition pesticide containing Cynanchum Komarovii and sophora alopecuroides extract of the present invention can be used by common method, sprays on crop blade face.Preferably, when using, the present invention is diluted with water to the activity component concentration of crude drug weighing scale as 10 ~ 100ppm containing the composition pesticide of Cynanchum Komarovii and sophora alopecuroides extract, namely the composition pesticide that 1g crude drug is obtained after extracting, when using, be diluted with water to 10kg, then its activity component concentration equals 100ppm; Preferably being diluted to activity component concentration is further that 20 ~ 50ppm sprays in plant blade face, and so that water membrane to appear in blade face, not lower of liquid is advisable; Secondary dispenser is carried out, the preferred continuous administration of a season crop 2 ~ 3 times after being preferable over 3 ~ 5 days.
Composition pesticide containing Cynanchum Komarovii and sophora alopecuroides extract of the present invention is mixed by Cynanchum Komarovii extract and sophora alopecuroides extract, or jointly extracted by Cynanchum Komarovii and Sophora alopecuroide and form, its preventive effect is far away higher than the extract of single component, particularly extract common after Cynanchum Komarovii and Sophora alopecuroide mixing, owing to having solubilising, hydrotropy effect between Cynanchum Komarovii and Sophora alopecuroide, the composition pesticide insecticidal effect made after common extraction is more remarkable.
Compared with prior art, the composition pesticide containing Cynanchum Komarovii and sophora alopecuroides extract of the present invention has the following advantages:
1, the composition pesticide containing Cynanchum Komarovii and sophora alopecuroides extract of the present invention has synergistic function, obvious to the control efficiency of the insect such as Homoptera, Semiptera.
2, Cynanchum Komarovii of the present invention and Sophora alopecuroide mix after jointly extracting or extracting respectively, and the composition pesticide made can improve crop immune system ability while quick-acting tagging, and can significantly improve its vigor.
3, the present invention take Chinese herbal medicine as raw material, thus there is not Pesticide Residue, and insect is difficult to produce resistance; Be used alone compared with chemical source agricultural chemicals, environmentally friendly.
4, when the composition pesticide containing Cynanchum Komarovii and sophora alopecuroides extract of the present invention uses, valid density is low, with low cost.
Embodiment
Below in conjunction with embodiment, the specific embodiment of the present invention is further described, does not therefore limit the present invention among described scope of embodiments.
Embodiment 1 contains the preparation of the composition pesticide of Cynanchum Komarovii and sophora alopecuroides extract
A, extraction: take dry Cynanchum Komarovii cauline leaf and Sophora alopecuroide stem stalk, mixing, pulverizes, and crosses 80 mesh sieves, add extraction solution, refluxing extraction twice, filter, merging filtrate, concentrated, make extract weight equal the weight of crude drug, obtain extract jy-01 ~ jy-06; Wherein, each parameter is in table 1;
B, preparation: in extract jy-01 ~ jy-06, add auxiliary agent in table 2, stir, must contain the composition pesticide JY-01 ~ JY-06 of Cynanchum Komarovii and sophora alopecuroides extract.
The preparation technology parameter of table 1 Cynanchum Komarovii and sophora alopecuroides extract
Wherein, Cynanchum Komarovii cauline leaf and Sophora alopecuroide stem stalk are all purchased from Anguo City Zhong Tian prepared slices of Chinese crude drugs Co., Ltd.
Table 2 contains the formula of the composition pesticide of Cynanchum Komarovii and sophora alopecuroides extract
Embodiment 2 contains the preparation of the composition pesticide of Cynanchum Komarovii and sophora alopecuroides extract
A, extraction: take dry Cynanchum Komarovii cauline leaf, pulverize, and crosses 80 mesh sieves, add extraction solution, refluxing extraction twice, filters, merging filtrate, and concentrated, make extract weight equal the weight of crude drug, obtain extract d1-01 ~ d1-03, wherein, each parameter is in table 3;
Take dry Sophora alopecuroide stem stalk, pulverize, cross 80 mesh sieves, add extraction solution, refluxing extraction twice, filter, merging filtrate, concentrated, make extract weight equal the weight of crude drug, obtain extract d2-01 ~ d2-03, wherein, each parameter is in table 4;
B, preparation: take Cynanchum Komarovii extract, Sophora alopecuroide extract and auxiliary agent, stirring and evenly mixing, obtain the composition pesticide JY-07 ~ JY-12 containing Cynanchum Komarovii and sophora alopecuroides extract, wherein, each parameter is in table 5.
Table 3
Table 4
Table 5
Test example 1 the present invention contains the efficiency test of the composition pesticide orange caterpillar of Cynanchum Komarovii and sophora alopecuroides extract
1, test material
1.1 for studying thing and object: orange caterpillar
1.2 reagent agent
Product in embodiment: JY-01 ~ JY-12;
Cynanchum Komarovii single dose (D1), Sophora alopecuroide single dose (D2);
Chemical reagent contrasts: 10% Methomyl Imidacloprid (CK-01), purchased from Hailir Pharmaceutical Group Co., Ltd.;
Clear water contrast (CK-02).
Wherein, the preparation method of Cynanchum Komarovii single dose is: the d1-03 getting 30g, adds 20g absolute ethyl alcohol, 15g emulsifier 508,5g emulsifier 506,5g methyl-sulfoxide, and 5g ethylene glycol, 20g water, stirring and evenly mixing, to obtain final product.
The preparation method of Sophora alopecuroide single dose is: the d2-03 getting 30g, adds 20g absolute ethyl alcohol, 15g emulsifier 508,5g emulsifier 506,5g methyl-sulfoxide, and 5g ethylene glycol, 20g water, stirring and evenly mixing, to obtain final product.
2, experimental scheme and method
This test 12 medicaments, 2 single dose contrasts, 1 contrast medicament, 1 blank, repeats for three times, Gong48Ge community.Minizone and surrounding all leave isolation strip, plot area 20m
2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the Aphed population statistics also listing mark of blade before the test.Within 5 days after dispenser, investigate and record the aphid number alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
3, result of the test
Each group is repeated meter of averaging, and calculate Revision insect recluced rate and preventive effect, it the results are shown in Table 6.
Table 6
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| JY-01 | 385 | 75 | 80.52 | 82.93 |
| JY-02 | 416 | 69 | 83.41 | 85.47 |
| JY-03 | 377 | 79 | 79.05 | 81.64 |
| JY-04 | 369 | 80 | 78.32 | 81.00 |
| JY-05 | 395 | 69 | 82.53 | 84.69 |
| JY-06 | 413 | 71 | 82.81 | 84.94 |
| JY-07 | 402 | 90 | 77.61 | 80.38 |
| JY-08 | 382 | 99 | 74.08 | 77.29 |
| JY-09 | 390 | 105 | 73.08 | 76.41 |
| JY-10 | 379 | 121 | 68.07 | 72.03 |
| JY-11 | 368 | 101 | 72.55 | 75.95 |
| JY-12 | 371 | 109 | 70.62 | 74.26 |
| D1 | 367 | 192 | 47.68 | 54.16 |
| D2 | 392 | 213 | 45.66 | 52.39 |
| CK-01 | 381 | 61 | 83.99 | 85.97 |
| CK-02 | 375 | 428 | -14.13 | -- |
As can be seen from Table 6, product of the present invention has good preventive effect to aphid, and better, visible than the control efficiency of single Cynanchum Komarovii extract and sophora alopecuroides extract, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.In addition, have no during dispenser and have poisoning to show to crop.
Test example 2 the present invention contains the efficiency test of the composition pesticide eggplant trialeurodes vaporariorum of Cynanchum Komarovii and sophora alopecuroides extract
Product J Y-01 of the present invention ~ JY-12 is used for preventing and treating eggplant trialeurodes vaporariorum.This test 12 medicaments, 2 single doses, 1 blank, repeats for three times, Gong45Ge community.Minizone and surrounding all leave isolation strip, plot area 10m
2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the trialeurodes vaporariorum quantity statistics alive of blade before the test and listing mark.Within 5 days after dispenser, investigate and record the trialeurodes vaporariorum quantity alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group is repeated meter of averaging, and calculate Revision insect recluced rate and preventive effect, it the results are shown in Table 7.
Table 7
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| JY-01 | 201 | 56 | 72.14 | 75.78 |
| JY-02 | 198 | 60 | 69.70 | 73.65 |
| JY-03 | 234 | 65 | 72.22 | 75.85 |
| JY-04 | 216 | 72 | 66.67 | 71.02 |
| JY-05 | 186 | 66 | 64.52 | 69.15 |
| JY-06 | 253 | 67 | 73.52 | 76.98 |
| JY-07 | 213 | 80 | 62.44 | 67.35 |
| JY-08 | 216 | 79 | 63.43 | 68.20 |
| JY-09 | 223 | 83 | 62.78 | 67.64 |
| JY-10 | 218 | 91 | 58.26 | 63.71 |
| JY-11 | 199 | 85 | 57.29 | 62.87 |
| JY-12 | 218 | 81 | 62.84 | 67.70 |
| D1 | 220 | 149 | 32.27 | 41.12 |
| D2 | 209 | 137 | 34.45 | 43.01 |
| CK-02 | 213 | 245 | 15.02 | -- |
As can be seen from Table 7, product of the present invention has good preventive effect to trialeurodes vaporariorum, and better, visible than the control efficiency of single Cynanchum Komarovii extract and sophora alopecuroides extract, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.In addition, have no during dispenser and have poisoning to show to crop.
Test example 3 the present invention contains the efficiency test of the composition pesticide oranges and tangerines cottonycushion scale of Cynanchum Komarovii and sophora alopecuroides extract
Product J Y-01 of the present invention ~ JY-12 is used for preventing and treating oranges and tangerines cottonycushion scale.This test 12 medicaments, 2 single doses, 1 blank, repeats for three times, Gong45Ge community.Minizone and surrounding all leave isolation strip, plot area 20m
2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the cottonycushion scale quantity statistics alive of blade before the test and listing mark.Within 5 days after dispenser, to investigate and continuous betweenness is blown in the work recorded on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group is repeated meter of averaging, and calculate Revision insect recluced rate and preventive effect, its result is as shown in table 8.
Table 8
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| JY-01 | 192 | 42 | 78.13 | 81.21 |
| JY-02 | 225 | 55 | 75.56 | 79.00 |
| JY-03 | 231 | 53 | 77.06 | 80.29 |
| JY-04 | 205 | 46 | 77.56 | 80.73 |
| JY-05 | 218 | 54 | 75.23 | 78.72 |
| JY-06 | 197 | 51 | 74.11 | 77.76 |
| JY-07 | 189 | 75 | 60.32 | 65.91 |
| JY-08 | 208 | 77 | 62.98 | 68.20 |
| JY-09 | 239 | 79 | 66.95 | 71.61 |
| JY-10 | 245 | 79 | 67.76 | 72.30 |
| JY-11 | 226 | 74 | 67.26 | 71.87 |
| JY-12 | 249 | 80 | 67.87 | 72.40 |
| D1 | 213 | 138 | 35.21 | 44.35 |
| D2 | 258 | 140 | 45.74 | 53.39 |
| CK-02 | 201 | 234 | -16.42 | -- |
As can be seen from Table 8, product of the present invention has good preventive effect to cottonycushion scale, and better, visible than the control efficiency of single Cynanchum Komarovii extract and sophora alopecuroides extract, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.In addition, have no during dispenser and have poisoning to show to crop.
Test example 4 the present invention contains the efficiency test of the composition pesticide grape mirid pentatomid of Cynanchum Komarovii and sophora alopecuroides extract
Product J Y-01 of the present invention ~ JY-12 is used for preventing and treating grape mirid pentatomid.This test 12 medicaments, 2 single doses, 1 blank, repeats for three times, Gong45Ge community.Minizone and surrounding all leave isolation strip, plot area 10m
2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the mirid pentatomid quantity statistics alive of blade before the test and listing mark.Within 5 days after dispenser, investigate and record the mirid pentatomid number alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group is repeated meter of averaging, and calculate Revision insect recluced rate and preventive effect, its result is as shown in table 9.
Table 9
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| JY-01 | 105 | 22 | 79.05 | 82.90 |
| JY-02 | 113 | 21 | 81.42 | 84.84 |
| JY-03 | 89 | 15 | 83.15 | 86.25 |
| JY-04 | 103 | 19 | 81.55 | 84.95 |
| JY-05 | 105 | 22 | 79.05 | 82.90 |
| JY-06 | 97 | 21 | 78.35 | 82.33 |
| JY-07 | 124 | 32 | 74.19 | 78.94 |
| JY-08 | 111 | 33 | 70.27 | 75.74 |
| JY-09 | 130 | 34 | 73.85 | 78.66 |
| JY-10 | 115 | 30 | 73.91 | 78.71 |
| JY-11 | 107 | 34 | 68.22 | 74.07 |
| JY-12 | 102 | 31 | 69.61 | 75.20 |
| D1 | 112 | 72 | 35.71 | 47.54 |
| D2 | 99 | 66 | 33.33 | 45.60 |
| CK-02 | 102 | 125 | -22.55 | - |
As can be seen from Table 9, product of the present invention has good preventive effect to mirid pentatomid, and better, visible than the control efficiency of single Cynanchum Komarovii extract and sophora alopecuroides extract, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.In addition, have no during dispenser and have poisoning to show to crop.
Claims (10)
1. contain the composition pesticide of Cynanchum Komarovii and sophora alopecuroides extract, it is characterized in that, its active component is extracted by the crude drug of following weight portion and forms: Cynanchum Komarovii 1 part, Sophora alopecuroide 0.5 ~ 6 part.
2. the composition pesticide containing Cynanchum Komarovii and sophora alopecuroides extract according to claim 1, is characterized in that, its active component is extracted by the crude drug of following weight portion and forms: Cynanchum Komarovii 1 part, Sophora alopecuroide 0.5 ~ 5 part.
3. the composition pesticide containing Cynanchum Komarovii and sophora alopecuroides extract according to claim 1, is characterized in that: after its active component is mixed by Cynanchum Komarovii and Sophora alopecuroide, common extraction forms; Or extract by Cynanchum Komarovii the Cynanchum Komarovii extract, the Sophora alopecuroide that obtain and extract the sophora alopecuroides extract obtained and mix.
4. the composition pesticide containing Cynanchum Komarovii and sophora alopecuroides extract according to any one of claims 1 to 3, is characterized in that: the described composition pesticide containing Cynanchum Komarovii and sophora alopecuroides extract is microemulsion, soluble concentrate or aqua.
5. the composition pesticide containing Cynanchum Komarovii and sophora alopecuroides extract according to any one of Claims 1 to 4, it is characterized in that: described containing in the composition pesticide of Cynanchum Komarovii and sophora alopecuroides extract also comprises auxiliary agent, and described auxiliary agent is at least one in emulsifier, antifreeze, solvent, defoamer, stabilizing agent.
6. the composition pesticide containing Cynanchum Komarovii and sophora alopecuroides extract according to claim 4, it is characterized in that: the described composition pesticide containing Cynanchum Komarovii and sophora alopecuroides extract is microemulsion, described microemulsion is made up of the component of following weight portion: Cynanchum Komarovii and sophora alopecuroides extract, 20 parts of absolute ethyl alcohols, 15 parts of fatty alcohol-polyoxyethylene ether, 5 parts of emulsifier alkyl benzene calcium sulfonates, 5 parts of ethylene glycol, 25 parts of water; Wherein, Cynanchum Komarovii and sophora alopecuroides extract be 30 parts crude drug extract form; Or:
The described composition pesticide containing Cynanchum Komarovii and sophora alopecuroides extract is aqua, described aqua is made up of the component of following weight portion: Cynanchum Komarovii and sophora alopecuroides extract, 5 parts of absolute ethyl alcohols, 5 parts of emulsifier styryl phenyl APEOs, 5 parts of ethylene glycol, 35 parts of water; Wherein, Cynanchum Komarovii and sophora alopecuroides extract be 50 parts crude drug extract form; Or:
The described composition pesticide containing Cynanchum Komarovii and sophora alopecuroides extract is microemulsion, described microemulsion is made up of the component of following weight portion: Cynanchum Komarovii and sophora alopecuroides extract, 10 parts of absolute ethyl alcohols, 15 parts of emulsifier op-10,3 parts of emulsifier calcium dodecyl benzene sulfonates, 5 parts of ethylene glycol, 5 parts of dimethyl sulfoxide (DMSO)s, 12 parts of water; Wherein, Cynanchum Komarovii and sophora alopecuroides extract be 50 parts crude drug extract form.
7. prepare the method containing the composition pesticide of Cynanchum Komarovii and sophora alopecuroides extract described in claim 1 ~ 6, it is characterized in that being made up of following steps:
A, extraction: take Cynanchum Komarovii particle and Sophora alopecuroide particle, mixing, adds solvent by solid-liquid ratio 1:3 ~ 12g/ml, and in 50 ~ 100 DEG C of refluxing extraction 2 ~ 3 hours, filter, obtain extract, wherein, described solvent was organic solvent or water;
B, preparation: extract is made microemulsion, aqueous emulsion, soluble concentrate or aqua.
8. preparation according to claim 7 contains the method for the composition pesticide of Cynanchum Komarovii and sophora alopecuroides extract, and it is characterized in that: in described a step, Cynanchum Komarovii particle and Sophora alopecuroide particle cross 60 ~ 100 mesh sieves, preferred mistake 80 mesh sieve; Solid-liquid ratio is 1:4 ~ 8g/ml; Organic solvent is methyl alcohol, ethanol, ethyl acetate, acetone, chloroform; Reflux temperature is for being 75 ~ 85 DEG C; Reflow's cycle is twice, and merging filtrate, obtains extract.
9. the composition pesticide containing Cynanchum Komarovii and sophora alopecuroides extract described in claim 1 ~ 6 is preventing and treating the application in crop pest.
10. the application of composition pesticide in control crop pest containing Cynanchum Komarovii and sophora alopecuroides extract according to claim 9, is characterized in that: described insect pest is Homoptera, Hemipteran pest; During application, be diluted with water to the activity component concentration of crude drug weighing scale for 10 ~ 100ppm, preferably 20 ~ 50ppm.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107114416A (en) * | 2017-06-07 | 2017-09-01 | 江苏徐淮地区淮阴农业科学研究所 | Prevent and treat the botanical fungicide of rice blast |
| CN107173398A (en) * | 2017-06-07 | 2017-09-19 | 江苏徐淮地区淮阴农业科学研究所 | Prevent and treat the botanical fungicide of wheat scab |
| CN108991030A (en) * | 2018-10-17 | 2018-12-14 | 内蒙古联硕生物医药研究所 | A kind of aphids resistance and the botanical pesticide microemulsion of locust and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1339260A (en) * | 2000-08-17 | 2002-03-13 | 阎德礼 | Biological powdery seed coating agent and its preparing method |
| CN102657243A (en) * | 2012-05-04 | 2012-09-12 | 宁夏大学 | Plant alkaloid compound microemulsion and preparation method of plant alkaloid compound microemulsion |
-
2015
- 2015-12-04 CN CN201510885131.7A patent/CN105284914A/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1339260A (en) * | 2000-08-17 | 2002-03-13 | 阎德礼 | Biological powdery seed coating agent and its preparing method |
| CN102657243A (en) * | 2012-05-04 | 2012-09-12 | 宁夏大学 | Plant alkaloid compound microemulsion and preparation method of plant alkaloid compound microemulsion |
Non-Patent Citations (2)
| Title |
|---|
| 冯建国等: "《无公害果品生产技术 修订版》", 30 June 2008, 金盾出版社 * |
| 张青文等: "《农业入侵害虫的可持续治理》", 30 April 2013, 中国农业大学出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107114416A (en) * | 2017-06-07 | 2017-09-01 | 江苏徐淮地区淮阴农业科学研究所 | Prevent and treat the botanical fungicide of rice blast |
| CN107173398A (en) * | 2017-06-07 | 2017-09-19 | 江苏徐淮地区淮阴农业科学研究所 | Prevent and treat the botanical fungicide of wheat scab |
| CN108991030A (en) * | 2018-10-17 | 2018-12-14 | 内蒙古联硕生物医药研究所 | A kind of aphids resistance and the botanical pesticide microemulsion of locust and preparation method thereof |
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Address after: 611630 No. five, No. 35 Industrial Road, Heshan Town, Pujiang County, Sichuan, Chengdu Applicant after: Chengdu new Chaoyang Crop Science Co., Ltd Address before: 611630 No. five, No. 35 Industrial Road, Heshan Town, Pujiang County, Sichuan, Chengdu Applicant before: CHENGDU NEWSUN CROPSCIENCE Co.,Ltd. |
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Application publication date: 20160203 |