CN103651596A - Pesticide composition containing lightyellow sophora root and sixpetal tailgrape extracts, and preparation method and application thereof - Google Patents
Pesticide composition containing lightyellow sophora root and sixpetal tailgrape extracts, and preparation method and application thereof Download PDFInfo
- Publication number
- CN103651596A CN103651596A CN201310744060.XA CN201310744060A CN103651596A CN 103651596 A CN103651596 A CN 103651596A CN 201310744060 A CN201310744060 A CN 201310744060A CN 103651596 A CN103651596 A CN 103651596A
- Authority
- CN
- China
- Prior art keywords
- seng
- kuh
- extract
- artabotrys odoratissimus
- artabotrys
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a pesticide composition containing lightyellow sophora root and sixpetal tailgrape extracts, and a preparation method and application thereof, and belongs to the field of pesticides. In order to solve technical problems, the invention provides the pesticide composition containing the lightyellow sophora root and sixpetal tailgrape extracts, and the preparation method and application thereof. According to the pesticide composition containing the lightyellow sophora root and sixpetal tailgrape extracts, active ingredients of the pesticide composition are extracted from 1 weight part of lightyellow sophora root and 1 to 10 weight parts of sixpetal tailgrape; the active ingredient can be prepared by mixing the lightyellow sophora root and the sixpetal tailgrape and extracting together, or prepared by separately extracting the lightyellow sophora root to obtain the lightyellow sophora root extract, separately extracting the sixpetal tailgrape to obtain the sixpetal tailgrape extract, and mixing the two extracts. The pesticide composition containing the lightyellow sophora root and sixpetal tailgrape extracts has an obvious effect of controlling Homoptera, Acarina, Thysanoptera and Lepidoptera pests, and has positive significance for agricultural production.
Description
Technical field
The present invention relates to composition pesticide and preparation method and application containing kuh-seng and Artabotrys odoratissimus extract, belong to pesticide field.
Background technology
In recent years, in agriculture and forestry field, due to single drug and Scientific Usage of Drugs not, plant pest has produced pesticide resistance in various degree, and agriculture and forestry are produced still with chemical pesticide as mainly preventing and treating kind, have caused the destruction increasingly of environmental pollution and ecotope.Along with the raising of people's living standard and the enhancing of health perception, the development of biopesticide and exploitation just progressively receive agricultural chemicals R&D worker's concern, become the focus of agriculture and forestry sustainable development, simultaneously, also be to improve environment, the effective way of minimizing or delaying drug resistance and raising drug effect.Wherein, botanical pesticide has become one of focus of biopesticide research.
Kuh-seng, the plant that pulse family kuh-seng belongs to.In its root, containing matrine, oxymatrine, N-, be oxidized the multiple alkaloids such as sophocarpine, Sophoridine, Iosmatrine, counterfeit indigo leaf alkali; Also containing multiple flavonoidss such as the new alcohol of kuh-seng, kuh-seng chalcone, kuh-seng alcohol, formoononetin, kurarinone, maackiain, kushenin, Xanthohumol, different fulvic acids; In addition, also contain triterpenoid saponin and quinones.Find after deliberation, kuh-seng has the effect of anti-inflammation, and Propiobacterium, staphylococcus aureus, Escherichia coli are all had to inhibitory action.In recent years, matrine is extensive use in agricultural, and has good control efficiency, is a kind of low toxicity, low-residual, environmental protection type agricultural chemical.
Artabotrys odoratissimus, have another name called the claws of a hawk, five pawls are blue, the claws of a hawk are blue, warbler pawl, shank are blue, claws of a hawk peach, for Annonaceae Artabotrys odoratissimus platymiscium, in Artabotrys odoratissimus plant, contain the chemical compositions such as claws of a hawk element (as claws of a hawk element A, claws of a hawk element B etc.), liriodendrin, atherospermidine and luteanine, there are some researches show, Artabotrys odoratissimus has the effect of preventing malaria.
In test, the present inventor finds that kuh-seng and the common extract of Artabotrys odoratissimus and shrubby sophora extract and Artabotrys odoratissimus extract mix and have good synergistic function, can be prepared into insecticide, aphid, scale insect, tetranychid, aleyrodid, thrips, cabbage caterpillar, diamond-back moth, prodenia litura etc. are had to good preventive effect.
Summary of the invention
The technical problem to be solved in the present invention is to provide composition pesticide and the preparation method and application containing kuh-seng and Artabotrys odoratissimus extract.
The present invention is containing the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, and its active component is formed by the crude drug extraction of following weight portion: 1 part of kuh-seng, 1～10 part of Artabotrys odoratissimus.Wherein, active component is preferably formed by the crude drug extraction of following weight portion: 1 part of kuh-seng, 3～8 parts of Artabotrys odoratissimus; The crude drug extraction being preferably again by following weight portion forms: 1 part of kuh-seng, 3～6 parts of Artabotrys odoratissimus; The more preferably crude drug extraction by following weight portion forms: 1 part of kuh-seng, 5 parts of Artabotrys odoratissimus.
Wherein, kuh-seng and Artabotrys odoratissimus that the present invention adopts are Chinese herbal medicine, and in order to improve the present invention containing the activity of the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, kuh-seng is dry radix sophorae preferably, and Artabotrys odoratissimus is dry claws of a hawk floral leaf preferably.
The present invention is containing the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, and after its active component can be mixed by crude drug kuh-seng and Artabotrys odoratissimus, common extraction forms; Also can first kuh-seng be extracted and obtain shrubby sophora extract separately, Artabotrys odoratissimus extracts and obtains Artabotrys odoratissimus extract separately, then two kinds of extracts are mixed.In order to improve the insecticidal properties of composition pesticide of the present invention, after its active component is preferably and is mixed by kuh-seng and Artabotrys odoratissimus, common extraction forms.
Further, in order to improve the stability containing the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, in the composition pesticide of the present invention containing kuh-seng and Artabotrys odoratissimus extract, also comprise auxiliary agent, described auxiliary agent can adopt the usual auxiliaries in agricultural chemicals, as at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
Wherein, emulsifier can adopt at least one in non-ionic surface active agent and anion surfactant, described non-ionic surface active agent can be dibenzyl xenyl APEO, styryl phenyl APEO, styryl phenyl APEO, phenethyl phenyl polyoxyethylene one polyethenoxy ether, OPEO, alkylphenol polyoxyethylene sulfosuccinate, aliphatic acid polyethenoxy ether, fatty alcohol-polyoxyethylene ether, aliphatic amine polyoxyethylene ether, castor oil ethylene oxide adduct and derivative thereof, at least one in polyol fatty acid ester and ethylene oxide adduct etc. thereof, described anion surfactant is at least one in calcium dodecyl benzene sulfonate, alkylphenol polyoxyethylene phosphate, phenethyl phenol polyethenoxy ether phosphate etc., as emulsifier 500,601p, 602p etc., in addition, can also use commercially available emulsifier production.
Wherein, solvent can adopt at least one in water, ethanol, isopropyl alcohol, n-butanol, ethyl acetate, butyl acetate, methyl-sulfoxide, turpentine oil, solvent naphtha, 1-METHYLPYRROLIDONE, dimethyl formamide etc.
Wherein, antifreeze can be glycerine, propane diols, ethylene glycol, sodium chloride, calcium chloride, sorbierite etc.
Wherein, defoamer is silicone defoaming agent etc.
Wherein, dispersant is at least one in lignosulfonates, PAA and propionamide copolymer, alkylnaphthalene sulfonate, polycarboxylic acid salt's condensation product, methyl sodiosul foaliphatate etc.
Wherein, wetting agent can be alkyl sulfate, alkylbenzenesulfonate, alkylnaphthalene sulfonate, aliphatic alcohol polyethenoxy base ether, alkylphenol-polyethenoxy base ether, sorbitan fatty acid ester etc.
Wherein, stabilizing agent can be 2-expoxy propane, butyl glycidyl ether, isopropyl alcohol, n-butanol, polyethylene glycol etc.
The present invention can be prepared into by the method for this area routine containing the composition pesticide of kuh-seng and Artabotrys odoratissimus extract any one formulation that is applicable to agricultural use, for the ease of storing and transportation, can make microemulsion, aqua or soluble concentrate.
Further, be preferably the microemulsion that the component by following weight portion forms: kuh-seng and Artabotrys odoratissimus extract, 15 parts of emulsifier op-10,500,5 parts of dimethyl sulfoxide (DMSO)s of 3 parts of emulsifier, 10 parts of ethanol, 5 parts of ethylene glycol, 22 parts of water, wherein, kuh-seng and Artabotrys odoratissimus extract are that the crude drug extraction of 40 parts forms.
For the ease of storing and transportation, can extract is concentrated, obtain concentrate, and then be prepared into various formulations, cycles of concentration can be adjusted voluntarily according to needs of production, as preferred version, kuh-seng is prepared from by the following method with Artabotrys odoratissimus extract: crude drug is identical with crude drug weight through extracting and being concentrated into.
Second technical problem that the present invention will solve is to provide preparation containing the method for the composition pesticide of kuh-seng and Artabotrys odoratissimus extract.
The present invention is comprised of following steps containing the preparation method of the composition pesticide of kuh-seng and Artabotrys odoratissimus extract:
A, extraction: take kuh-seng particle and Artabotrys odoratissimus particle, mix, by solid-liquid ratio, 1:3～12g/ml adds solvent, regulate pH to 5-7, in 60～100 ℃ of refluxing extraction, filter, obtain extract, wherein, described solvent is methyl alcohol, ethanol, ethyl acetate, acetone, chloroform or water;
B, preparation: extract is made to microemulsion, soluble concentrate or aqua.
Wherein, kuh-seng particle and Artabotrys odoratissimus particle can adopt commercially available particle, also can adopt conventional method pulverizing radix sophorae and claws of a hawk floral leaf to obtain.In order to improve the effect of extraction, simultaneously cost-saving, kuh-seng particle and Artabotrys odoratissimus particle are crossed 60～100 mesh sieves, preferred mistake 80 mesh sieves.
Further, for cost-saving, simultaneously also in order to improve extraction efficiency, the solid-liquid ratio in a step is preferably 1:4～10g/ml, more preferably 1:6g/ml.The kuh-seng and the Artabotrys odoratissimus particle that are lg can add 3～12ml solvent extraction, preferably add 4～10ml, more preferably add 6ml.
Conventional organic solvent all can be used for the present invention, for cost-saving, improves dissolution rate, guarantees extraction efficiency simultaneously, and solvent is preferably purified water, and reflux temperature is preferably 100 ℃, and pH value is preferably 6.0.For cost-saving, in preparation method of the present invention, regulating the material of pH to be selected from mass fraction is the sodium hydroxide solution that 1～10% hydrochloric acid solution or mass fraction are 1～10%.
In order to shorten preparation time, accelerate extraction rate, can when adding hot reflux, stir, mixing speed is preferably 80～100 revs/min.
Further, reflow's cycle is twice, and each return time is 2 hours, and merging filtrate, obtains extract.
The present invention also provides the application in control crop pest of composition pesticide that the present invention contains kuh-seng and Artabotrys odoratissimus extract.The present invention is containing the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, to homoptera pest as aphid, scale insect, trialeurodes vaporariorum etc.; Acarina pest is as panonychus citri, Tetranychus cinnabarinus etc.; Thrips insect is as thrips; Lepidoptera pest has good preventive effect as cabbage caterpillar, diamond-back moth, prodenia litura, beet snout moth's larva, tea tree tea geometrid etc.
In actual agricultural production, in order to improve composition pesticide containing kuh-seng and the Artabotrys odoratissimus extract control efficiency to insect pest, the described composition pesticide containing kuh-seng and Artabotrys odoratissimus extract is preferably applied to crop pest initial stage of origination; The present invention can use by common method containing the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, sprays on crop blade face.As preferred version, while using, the present invention is diluted with water to containing the composition pesticide of kuh-seng and Artabotrys odoratissimus extract to take the activity component concentration of crude drug weighing scale be 10～100ppm, it is the composition pesticide making after 1g crude drug extracts, when using, be diluted with water to 10kg, its activity component concentration equals 100ppm.Further preferably being diluted to activity component concentration is that 20～50ppm sprays in plant blade face, and to occur water membrane on blade face, not lower of liquid is advisable; After being preferable over 3～5 days, carry out secondary dispenser, the preferred continuous administration of a season crop 2～3 times.
The present invention is mixed by shrubby sophora extract and Artabotrys odoratissimus extract containing the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, or formed by kuh-seng and common extraction of Artabotrys odoratissimus, its preventive effect is far away higher than the extract of single component, particularly kuh-seng and Artabotrys odoratissimus are mixed to rear common extraction, owing to having solubilising, hydrotropy effect between kuh-seng and Artabotrys odoratissimus, the composition pesticide insecticidal effect of making after common extraction is more remarkable.
Compared with prior art, the present invention has the following advantages containing the composition pesticide of kuh-seng and Artabotrys odoratissimus extract:
1, the present invention has synergistic function containing the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, obvious to the control efficiency of Homoptera, Acarina, thrips, lepidoptera pest.
2, kuh-seng of the present invention and Artabotrys odoratissimus are common extracts or extracts respectively rear mixing, and the composition pesticide of making can improve crop immune system ability in quick-acting tagging, and can significantly improve its vigor.
3, the present invention be take Chinese herbal medicine as raw material, thereby does not have Pesticide Residue, and insect is difficult to produce resistance; Compare with independent use chemical source agricultural chemicals, environmentally friendly.
When 4, the present invention uses containing the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, valid density is low, with low cost.
Embodiment
The present invention is containing the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, and its active component is formed by the crude drug extraction of following weight portion: 1 part of kuh-seng, 1～10 part of Artabotrys odoratissimus.Wherein, active component is preferably formed by the crude drug extraction of following weight portion: 1 part of kuh-seng, 3～8 parts of Artabotrys odoratissimus; The crude drug extraction being preferably again by following weight portion forms: 1 part of kuh-seng, 3～6 parts of Artabotrys odoratissimus; The more preferably crude drug extraction by following weight portion forms: 1 part of kuh-seng, 5 parts of Artabotrys odoratissimus.
Wherein, kuh-seng and Artabotrys odoratissimus that the present invention adopts are Chinese herbal medicine, and in order to improve the present invention containing the activity of the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, kuh-seng is dry radix sophorae preferably, and Artabotrys odoratissimus is dry claws of a hawk floral leaf preferably.
The present invention is containing the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, and after its active component can be mixed by crude drug kuh-seng and Artabotrys odoratissimus, common extraction forms; Also can first kuh-seng be extracted and obtain shrubby sophora extract separately, Artabotrys odoratissimus extracts and obtains Artabotrys odoratissimus extract separately, then two kinds of extracts are mixed.In order to improve the insecticidal properties of composition pesticide of the present invention, after its active component is preferably and is mixed by kuh-seng and Artabotrys odoratissimus, common extraction forms.
Further, in order to improve the stability containing the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, in the composition pesticide of the present invention containing kuh-seng and Artabotrys odoratissimus extract, also comprise auxiliary agent, described auxiliary agent can adopt the usual auxiliaries in agricultural chemicals, as at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
Wherein, emulsifier can adopt at least one in non-ionic surface active agent and anion surfactant, described non-ionic surface active agent can be dibenzyl xenyl APEO, styryl phenyl APEO, styryl phenyl APEO, phenethyl phenyl polyoxyethylene one polyethenoxy ether, OPEO, alkylphenol polyoxyethylene sulfosuccinate, aliphatic acid polyethenoxy ether, fatty alcohol-polyoxyethylene ether, aliphatic amine polyoxyethylene ether, castor oil ethylene oxide adduct and derivative thereof, at least one in polyol fatty acid ester and ethylene oxide adduct etc. thereof, described anion surfactant is at least one in calcium dodecyl benzene sulfonate, alkylphenol polyoxyethylene phosphate, phenethyl phenol polyethenoxy ether phosphate etc., as emulsifier 500,601p, 602p etc., in addition, can also use commercially available emulsifier production.
Wherein, solvent can adopt at least one in water, ethanol, isopropyl alcohol, n-butanol, ethyl acetate, butyl acetate, methyl-sulfoxide, turpentine oil, solvent naphtha, 1-METHYLPYRROLIDONE, dimethyl formamide etc.
Wherein, antifreeze can be glycerine, propane diols, ethylene glycol, sodium chloride, calcium chloride, sorbierite etc.
Wherein, defoamer is silicone defoaming agent etc.
Wherein, dispersant is at least one in lignosulfonates, PAA and propionamide copolymer, alkylnaphthalene sulfonate, polycarboxylic acid salt's condensation product, methyl sodiosul foaliphatate etc.
Wherein, wetting agent can be alkyl sulfate, alkylbenzenesulfonate, alkylnaphthalene sulfonate, aliphatic alcohol polyethenoxy base ether, alkylphenol-polyethenoxy base ether, sorbitan fatty acid ester etc.
Wherein, stabilizing agent can be 2-expoxy propane, butyl glycidyl ether, isopropyl alcohol, n-butanol, polyethylene glycol etc.
The present invention can be prepared into by the method for this area routine containing the composition pesticide of kuh-seng and Artabotrys odoratissimus extract any one formulation that is applicable to agricultural use, for the ease of storing and transportation, can make microemulsion, aqua or soluble concentrate.
Further, be preferably the microemulsion that the component by following weight portion forms: kuh-seng and Artabotrys odoratissimus extract, 15 parts of emulsifier op-10,500,5 parts of dimethyl sulfoxide (DMSO)s of 3 parts of emulsifier, 10 parts of ethanol, 5 parts of ethylene glycol, 22 parts of water, wherein, kuh-seng and Artabotrys odoratissimus extract are that the crude drug extraction of 40 parts forms.
For the ease of storing and transportation, can extract is concentrated, obtain concentrate, and then be prepared into various formulations, cycles of concentration can be adjusted voluntarily according to needs of production, as preferred version, kuh-seng is prepared from by the following method with Artabotrys odoratissimus extract: crude drug is identical with crude drug weight through extracting and being concentrated into.
The present invention is comprised of following steps containing the preparation method of the composition pesticide of kuh-seng and Artabotrys odoratissimus extract:
A, extraction: take kuh-seng particle and Artabotrys odoratissimus particle, mix, by solid-liquid ratio, 1:3～12g/ml adds solvent, regulate pH to 5-7, in 60～100 ℃ of refluxing extraction, filter, obtain extract, wherein, described solvent is methyl alcohol, ethanol, ethyl acetate, acetone, chloroform or water;
B, preparation: extract is made to microemulsion, soluble concentrate or aqua.
Wherein, kuh-seng particle and Artabotrys odoratissimus particle can adopt commercially available particle, also can adopt conventional method pulverizing radix sophorae and claws of a hawk floral leaf to obtain.In order to improve the effect of extraction, simultaneously cost-saving, kuh-seng particle and Artabotrys odoratissimus particle are crossed 60～100 mesh sieves, preferred mistake 80 mesh sieves.
Further, for cost-saving, simultaneously also in order to improve extraction efficiency, the solid-liquid ratio in a step is preferably 1:4～10g/ml, more preferably 1:6g/ml.The kuh-seng and the Artabotrys odoratissimus particle that are lg can add 3～12ml solvent extraction, preferably add 4～10ml, more preferably add 6ml.
Conventional organic solvent all can be used for the present invention, for cost-saving, improves dissolution rate, guarantees extraction efficiency simultaneously, and solvent is preferably purified water, and reflux temperature is preferably 100 ℃, and pH value is preferably 6.0.For cost-saving, in preparation method of the present invention, regulating the material of pH to be selected from mass fraction is the sodium hydroxide solution that 1～10% hydrochloric acid solution or mass fraction are 1～10%.
In order to shorten preparation time, accelerate extraction rate, can when adding hot reflux, stir, mixing speed is preferably 80～100 revs/min.
Further, reflow's cycle is twice, and each return time is 2 hours, and merging filtrate, obtains extract.
The present invention is containing the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, to homoptera pest as aphid, scale insect, trialeurodes vaporariorum etc.; Acarina pest is as panonychus citri, Tetranychus cinnabarinus etc.; Thrips insect is as thrips; Lepidoptera pest has good preventive effect as cabbage caterpillar, diamond-back moth, prodenia litura, beet snout moth's larva, tea tree tea geometrid etc.
In actual agricultural production, in order to improve composition pesticide containing kuh-seng and the Artabotrys odoratissimus extract control efficiency to insect pest, the described composition pesticide containing kuh-seng and Artabotrys odoratissimus extract is preferably applied to crop pest initial stage of origination; The present invention can use by common method containing the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, sprays on crop blade face.As preferred version, while using, the present invention is diluted with water to containing the composition pesticide of kuh-seng and Artabotrys odoratissimus extract to take the activity component concentration of crude drug weighing scale be 10～100ppm, it is the composition pesticide making after 1g crude drug extracts, when using, be diluted with water to 10kg, its activity component concentration equals 100ppm.Further preferably being diluted to activity component concentration is that 20～50ppm sprays in plant blade face, and to occur water membrane on blade face, not lower of liquid is advisable; After being preferable over 3～5 days, carry out secondary dispenser, the preferred continuous administration of a season crop 2～3 times.
The present invention is mixed by shrubby sophora extract and Artabotrys odoratissimus extract containing the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, or formed by kuh-seng and common extraction of Artabotrys odoratissimus, its preventive effect is far away higher than the extract of single component, particularly kuh-seng and Artabotrys odoratissimus are mixed to rear common extraction, owing to having solubilising, hydrotropy effect between kuh-seng and Artabotrys odoratissimus, the composition pesticide insecticidal effect of making after common extraction is more remarkable.
Below in conjunction with embodiment, the specific embodiment of the present invention is further described, does not therefore limit the present invention among described scope of embodiments.
Embodiment 1 is containing the preparation of the composition pesticide of kuh-seng and Artabotrys odoratissimus extract
A, extraction: take dry radix sophorae and claws of a hawk floral leaf, mix, pulverize, cross 80 mesh sieves, add extraction solution, regulating pH value is 6.0, and refluxing extraction twice is filtered, merging filtrate, concentrated, make extract weight equal the weight of crude drug, obtain extract ky-01～ky-06; Wherein, each parameter is in Table 1;
B, preparation: auxiliary agent add table 2 in extract ky-01～ky-06 in, stir, must be containing the composition pesticide KY-01～KY-06 of kuh-seng and Artabotrys odoratissimus extract.
The preparation technology parameter of table 1 kuh-seng and Artabotrys odoratissimus extract
Wherein, radix sophorae and claws of a hawk floral leaf are all purchased from Anguo City Zhong Tian prepared slices of Chinese crude drugs Co., Ltd.
Table 2 is containing the formula of the composition pesticide of kuh-seng and Artabotrys odoratissimus extract
Embodiment 2 is containing the preparation of the composition pesticide of kuh-seng and Artabotrys odoratissimus extract
A, extraction: take dry radix sophorae, pulverize, cross 80 mesh sieves, add extraction solution, regulating pH value is 6.0, refluxing extraction twice, filters, and merging filtrate is concentrated, make extract weight equal the weight of crude drug, obtain extract d1-01～d1-03, wherein, each parameter is in Table 3;
Take dry claws of a hawk floral leaf, pulverize, cross 80 mesh sieves, add extraction solution, regulating pH value is 6.0, refluxing extraction twice, filters, and merging filtrate is concentrated, make extract weight equal the weight of crude drug, obtain extract d2-01～d2-03, wherein, each parameter is in Table 4;
B, preparation: take Radix Sophorae Flavescentis extractive liquid, Artabotrys odoratissimus extract and auxiliary agent, stirring and evenly mixing, obtains the composition pesticide KY-07～KY-12 containing kuh-seng and Artabotrys odoratissimus extract, and wherein, each parameter is in Table 5.
Table 3
Table 4
Table 5
Test example 1 the present invention is containing the efficiency test of the composition pesticide wild cabbage diamond-back moth of kuh-seng and Artabotrys odoratissimus extract
For clearly putting into field efficacy situation containing the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, inventor carries out field control effectiveness test to this medicament.
1, test material
1.1 for studying thing and object: pumpkin aphid
1.2 reagent agent
Product in embodiment: KY-01～KY-12;
Kuh-seng single dose (D1), Artabotrys odoratissimus single dose (D2):
Chemical reagent contrast (CK-01): 10% Methomyl Imidacloprid, purchased from Hailir Pharmaceutical Group Co., Ltd.;
Clear water contrast (CK-02).
Wherein, the preparation method of kuh-seng single dose is: get the d1-03 of 40g, add 15g emulsifier op-10, and 3g emulsifier 500,5g dimethyl sulfoxide (DMSO), 10g ethanol, 5g ethylene glycol, 22g water, stirring and evenly mixing, makes kuh-seng single dose.
The preparation method of Artabotrys odoratissimus single dose is: gets the d2-03 of 40g, adds 15g emulsifier op-10, and 3g emulsifier 500,5g dimethyl sulfoxide (DMSO), 10g ethanol, 5g ethylene glycol, 22g water, stirring and evenly mixing, makes Artabotrys odoratissimus single dose.
2, experimental scheme and method
14 medicaments of this test, 1 contrast medicament, 1 blank, repeats Gong48Ge community for three times.Minizone and surrounding all leave isolation strip, community area 12m
2, randomized arrangement is respectively processed in community.
By reagent agent thin up, to activity component concentration, be 50ppm, spray application to plant blade face, to occur water membrane on blade face, not lower of liquid is advisable.
Before test, carry out Aphed population statistics alive the listing mark of fixed blade.Within 5 days after dispenser, investigate and record the aphid number alive on each blade.Calculate insect population decline rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
3, result of the test
Within 5 days after dispenser, investigate and record the borer population alive on each blade, calculate insect population decline rate and preventive effect, it the results are shown in Table 6.
Table 6
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Insect population decline rate (%) | Preventive effect (%) |
| KY-01 | 313 | 61 | 80.51 | 83.22 |
| KY-02 | 322 | 43 | 86.65 | 88.50 |
| KY-03 | 353 | 41 | 88.39 | 90.00 |
| KY-04 | 344 | 43 | 87.50 | 89.24 |
| KY-05 | 367 | 54 | 85.29 | 87.33 |
| KY-06 | 325 | 52 | 84.00 | 86.22 |
| KY-07 | 374 | 100 | 73.26 | 76.98 |
| KY-08 | 396 | 90 | 77.27 | 80.43 |
| KY-09 | 389 | 85 | 78.15 | 81.19 |
| KY-10 | 370 | 83 | 77.57 | 80.68 |
| KY-11 | 358 | 91 | 74.58 | 78.11 |
| KY-12 | 343 | 88 | 74.34 | 77.91 |
| D1 | 338 | 216 | 36.09 | 44.98 |
| D2 | 361 | 260 | 27.98 | 37.99 |
| CK-01 | 388 | 41 | 89.43 | 90.90 |
| CK-02 | 316 | 367 | -16.14 | —— |
As can be seen from Table 6, product of the present invention has good preventive effect to aphid, and better, visible than the control efficiency of single shrubby sophora extract and Artabotrys odoratissimus extract, between product compositions of the present invention, has synergistic function.And the preventive effect that adopts the product that common extracting method makes is apparently higher than blended product after independent extraction, visible common extraction contributes to improve dissolution rate, improves insecticide efficiency.
Test example 2 the present invention are containing the efficiency test of the composition pesticide cucumber two spotted spider mite of kuh-seng and Artabotrys odoratissimus extract
Product K Y-01～KY-12 of the present invention is used for preventing and treating cucumber two spotted spider mite.12 medicaments of this test, 2 single doses, 1 blank, repeats Gong45Ge community for three times.Minizone and surrounding all leave isolation strip, community area 10m
2, randomized arrangement is respectively processed in community.
By reagent agent thin up, to activity component concentration, be 50ppm, spray application to plant blade face, to occur water membrane on blade face, not lower of liquid is advisable.
Before test, carry out two spotted spider mite quantity statistics alive the listing mark of fixed blade.Within 5 days after dispenser, investigate and record the two spotted spider mite number alive on each blade.Calculate insect population decline rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group meter that repeats to average, calculates insect population decline rate and preventive effect, and it the results are shown in Table 7.
Table 7
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Insect population decline rate (%) | Preventive effect (%) |
| KY-01 | 133 | 42 | 68.42 | 74.47 |
| KY-02 | 125 | 33 | 73.60 | 78.66 |
| KY-03 | 155 | 37 | 76.13 | 80.70 |
| KY-04 | 146 | 37 | 74.66 | 79.51 |
| KY-05 | 178 | 50 | 71.91 | 77.29 |
| KY-06 | 195 | 57 | 70.77 | 76.37 |
| KY-07 | 170 | 75 | 55.88 | 64.33 |
| KY-08 | 132 | 50 | 62.12 | 69.37 |
| KY-09 | 157 | 56 | 64.33 | 71.16 |
| KY-10 | 184 | 66 | 64.13 | 71.00 |
| KY-11 | 118 | 48 | 59.32 | 67.11 |
| KY-12 | 141 | 58 | 58.87 | 66.74 |
| D1 | 186 | 166 | 10.75 | 27.84 |
| D2 | 131 | 123 | 6.11 | 24.09 |
| CK-02 | 114 | 141 | -23.68 | —— |
As can be seen from Table 7, product of the present invention has good preventive effect to two spotted spider mite, and better, visible than the control efficiency of single shrubby sophora extract and Artabotrys odoratissimus extract, between product compositions of the present invention, has synergistic function.And the preventive effect that adopts the product that common extracting method makes is apparently higher than blended product after independent extraction, visible common extraction contributes to improve dissolution rate, improves insecticide efficiency.In addition, during dispenser, have no crop is had to poisoning performance.
Test example 3 the present invention are containing the efficiency test of the composition pesticide wild cabbage prodenia litura of kuh-seng and Artabotrys odoratissimus extract
Product K Y-01～KY-12 of the present invention is used for preventing and treating wild cabbage prodenia litura.12 medicaments of this test, 2 single doses, 1 blank, repeats Gong45Ge community for three times.Minizone and surrounding all leave isolation strip, community area 12m
2, randomized arrangement is respectively processed in community.
By reagent agent thin up, to activity component concentration, be 50ppm, spray application to plant blade face, to occur water membrane on blade face, not lower of liquid is advisable.
Before test, carry out prodenia litura quantity statistics alive the listing mark of fixed blade.Within 5 days after dispenser, investigate and record the prodenia litura number alive on each blade.Calculate insect population decline rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group meter that repeats to average, calculates insect population decline rate and preventive effect, and its result is as shown in table 8.
Table 8
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Insect population decline rate (%) | Preventive effect (%) |
| KY-01 | 245 | 63 | 74.29 | 77.85 |
| KY-02 | 231 | 46 | 80.09 | 82.85 |
| KY-03 | 245 | 42 | 82.86 | 85.23 |
| KY-04 | 235 | 46 | 80.43 | 83.14 |
| KY-05 | 275 | 59 | 78.55 | 81.52 |
| KY-06 | 290 | 65 | 77.59 | 80.69 |
| KY-07 | 201 | 75 | 62.69 | 67.86 |
| KY-08 | 228 | 67 | 70.61 | 74.69 |
| KY-09 | 240 | 66 | 72.50 | 76.31 |
| KY-10 | 231 | 67 | 71.00 | 75.02 |
| KY-11 | 262 | 82 | 68.70 | 73.04 |
| KY-12 | 274 | 94 | 65.69 | 70.45 |
| D1 | 237 | 180 | 24.05 | 34.58 |
| D2 | 216 | 173 | 19.91 | 31.02 |
| CK-02 | 236 | 274 | -16.10 | —— |
As can be seen from Table 8, product of the present invention has good preventive effect to prodenia litura, and better, visible than the control efficiency of single shrubby sophora extract and Artabotrys odoratissimus extract, between product compositions of the present invention, has synergistic function.And the preventive effect that adopts the product that common extracting method makes is apparently higher than blended product after independent extraction, visible common extraction contributes to improve dissolution rate, improves insecticide efficiency.In addition, during dispenser, have no crop is had to poisoning performance.
Test example 4 the present invention are containing the efficiency test of the composition pesticide wild cabbage diamond-back moth of kuh-seng and Artabotrys odoratissimus extract
Product K Y-01～KY-12 of the present invention is used for preventing and treating wild cabbage diamond-back moth.12 medicaments of this test, 2 single doses, 1 blank, repeats Gong45Ge community for three times.Minizone and surrounding all leave isolation strip, community area 10m
2, randomized arrangement is respectively processed in community.
By reagent agent thin up, to activity component concentration, be 50ppm, spray application to plant blade face, to occur water membrane on blade face, not lower of liquid is advisable.
Before test, carry out diamond-back moth quantity statistics alive the listing mark of fixed blade.Within 5 days after dispenser, investigate and record the diamond-back moth number alive on each blade.Calculate insect population decline rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group meter that repeats to average, calculates insect population decline rate and preventive effect, and its result is as shown in table 9.
Table 9
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Insect population decline rate (%) | Preventive effect (%) |
| KY-01 | 233 | 45 | 80.69 | 83.12 |
| KY-02 | 235 | 32 | 86.38 | 88.10 |
| KY-03 | 252 | 29 | 88.49 | 89.94 |
| KY-04 | 264 | 35 | 86.74 | 88.41 |
| KY-05 | 278 | 40 | 85.61 | 87.42 |
| KY-06 | 245 | 39 | 84.08 | 86.09 |
| KY-07 | 234 | 62 | 73.50 | 76.84 |
| KY-08 | 215 | 48 | 77.67 | 80.49 |
| KY-09 | 221 | 47 | 78.73 | 81.41 |
| KY-10 | 254 | 56 | 77.95 | 80.73 |
| KY-11 | 267 | 67 | 74.91 | 78.07 |
| KY-12 | 285 | 72 | 74.74 | 77.92 |
[0141]continued 9
| D1 | 290 | 136 | 53.10 | 59.01 |
| D2 | 253 | 159 | 37.15 | 45.07 |
| CK-02 | 215 | 246 | -14.42 | —— |
As can be seen from Table 9, product of the present invention has good preventive effect to diamond-back moth, and better, visible than the control efficiency of single shrubby sophora extract and Artabotrys odoratissimus extract, between product compositions of the present invention, has synergistic function.And the preventive effect that adopts the product that common extracting method makes is apparently higher than blended product after independent extraction, visible common extraction contributes to improve dissolution rate, improves insecticide efficiency.In addition, during dispenser, have no crop is had to poisoning performance.
Test example 5 the present invention are containing the efficiency test of the composition pesticide tomato thrips of kuh-seng and Artabotrys odoratissimus extract
Product K Y-01～KY-12 of the present invention is used for preventing and treating tomato thrips.12 medicaments of this test, 2 single doses, 1 blank, repeats Gong45Ge community for three times.Minizone and surrounding all leave isolation strip, community area 10m
2, randomized arrangement is respectively processed in community.
By reagent agent thin up, to activity component concentration, be 50ppm, spray application to plant blade face, to occur water membrane on blade face, not lower of liquid is advisable.
Before test, carry out thrips quantity statistics alive the listing mark of fixed blade.Within 5 days after dispenser, investigate and record the thrips number alive on each blade.Calculate insect population decline rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group meter that repeats to average, calculates insect population decline rate and preventive effect, and its result is as shown in table 10.
Table 10
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Insect population decline rate (%) | Preventive effect (%) |
| KY-01 | 143 | 39 | 72.73 | 77.10 |
| KY-02 | 156 | 33 | 78.85 | 82.24 |
| KY-03 | 124 | 24 | 80.65 | 83.75 |
| KY-04 | 127 | 27 | 78.74 | 82.15 |
| KY-05 | 174 | 41 | 76.44 | 80.21 |
[0155]continued 10
| KY-06 | 180 | 44 | 75.56 | 79.47 |
| KY-07 | 196 | 86 | 56.12 | 63.15 |
| KY-08 | 152 | 51 | 66.45 | 71.82 |
| KY-09 | 150 | 48 | 68.00 | 73.13 |
| KY-10 | 141 | 47 | 66.67 | 72.01 |
| KY-11 | 136 | 51 | 62.50 | 68.51 |
| KY-12 | 159 | 64 | 59.75 | 66.20 |
| D1 | 123 | 101 | 17.89 | 31.05 |
| D2 | 164 | 148 | 9.76 | 24.22 |
| CK-02 | 131 | 156 | -19.08 | —— |
As can be seen from Table 10, product of the present invention has good preventive effect to thrips, and better, visible than the control efficiency of single shrubby sophora extract and Artabotrys odoratissimus extract, between product compositions of the present invention, has synergistic function.And the preventive effect that adopts the product that common extracting method makes is apparently higher than blended product after independent extraction, visible common extraction contributes to improve dissolution rate, improves insecticide efficiency.In addition, during dispenser, have no crop is had to poisoning performance.
Test example 6 the present invention are containing the efficiency test of the composition pesticide oranges and tangerines cottonycushion scale of kuh-seng and Artabotrys odoratissimus extract
Product K Y-01～KY-12 of the present invention is used for preventing and treating oranges and tangerines cottonycushion scale.12 medicaments of this test, 2 single doses, 1 blank, repeats Gong45Ge community for three times.Minizone and surrounding all leave isolation strip, community area 20m
2, randomized arrangement is respectively processed in community.
By reagent agent thin up, to activity component concentration, be 50ppm, spray application to plant blade face, to occur water membrane on blade face, not lower of liquid is advisable.
Before test, carry out cottonycushion scale quantity statistics alive the listing mark of fixed blade.Within 5 days after dispenser, investigate and record the cottonycushion scale number alive on each blade.Calculate insect population decline rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group meter that repeats to average, calculates insect population decline rate and preventive effect, and its result is as shown in table 11.
Table 11
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Insect population decline rate (%) | Preventive effect (%) |
| KY-01 | 147 | 48 | 67.35 | 71.61 |
| KY-02 | 132 | 34 | 74.24 | 77.61 |
| KY-03 | 152 | 36 | 76.32 | 79.41 |
| KY-04 | 166 | 42 | 74.70 | 78.00 |
| KY-05 | 115 | 32 | 72.17 | 75.81 |
| KY-06 | 135 | 40 | 70.37 | 74.24 |
| KY-07 | 167 | 75 | 55.09 | 60.96 |
| KY-08 | 174 | 66 | 62.07 | 67.02 |
| KY-09 | 153 | 55 | 64.05 | 68.75 |
| KY-10 | 176 | 66 | 62.50 | 67.40 |
| KY-11 | 153 | 63 | 58.82 | 64.20 |
| KY-12 | 185 | 77 | 58.38 | 63.82 |
| D1 | 136 | 118 | 13.24 | 24.57 |
| D2 | 121 | 110 | 9.09 | 20.97 |
| CK-02 | 173 | 199 | -15.03 | —— |
As can be seen from Table 11, product of the present invention has good preventive effect to oranges and tangerines cottonycushion scale, and better, visible than the control efficiency of single shrubby sophora extract and Artabotrys odoratissimus extract, between product compositions of the present invention, has synergistic function.And the preventive effect that adopts the product that common extracting method makes is apparently higher than blended product after independent extraction, visible common extraction contributes to improve dissolution rate, improves insecticide efficiency.In addition, during dispenser, have no crop is had to poisoning performance.
Claims (10)
1. containing the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, it is characterized in that, its active component is formed by the crude drug extraction of following weight portion: 1 part of kuh-seng, 1～10 part of Artabotrys odoratissimus.
2. the composition pesticide containing kuh-seng and Artabotrys odoratissimus extract according to claim 1, is characterized in that, its active component is formed by the crude drug extraction of following weight portion: 1 part of kuh-seng, 3～8 parts of Artabotrys odoratissimus; Be preferably 1 part of kuh-seng, 3～6 parts of Artabotrys odoratissimus; More preferably kuh-seng is 1 part, 5 parts of Artabotrys odoratissimus.
3. the composition pesticide containing kuh-seng and Artabotrys odoratissimus extract according to claim 1 and 2, is characterized in that: after its active component is mixed by kuh-seng and Artabotrys odoratissimus, common extraction forms; Or by kuh-seng, extract the shrubby sophora extract, the Artabotrys odoratissimus that obtain and extract the Artabotrys odoratissimus extract obtaining and mix.
4. according to the composition pesticide containing kuh-seng and Artabotrys odoratissimus extract described in claim 1～3 any one, it is characterized in that: the described composition pesticide containing kuh-seng and Artabotrys odoratissimus extract is microemulsion, aqua or soluble concentrate.
5. according to the composition pesticide containing kuh-seng and Artabotrys odoratissimus extract described in claim 1～4 any one, it is characterized in that: in the described composition pesticide containing kuh-seng and Artabotrys odoratissimus extract, also comprise auxiliary agent, described auxiliary agent is at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
6. the composition pesticide containing kuh-seng and Artabotrys odoratissimus extract according to claim 5, it is characterized in that: described microemulsion is comprised of the component of following weight portion: kuh-seng and Artabotrys odoratissimus extract, 15 parts of emulsifier op-10,500,5 parts of dimethyl sulfoxide (DMSO)s of 3 parts of emulsifier, 10 parts of ethanol, 5 parts of ethylene glycol, 22 parts of water, wherein, kuh-seng and Artabotrys odoratissimus extract are that the crude drug extraction of 40 parts forms; Kuh-seng is preferably prepared from by the following method with Artabotrys odoratissimus extract: crude drug is identical with crude drug weight through extracting and being concentrated into.
7. the method for the composition pesticide containing kuh-seng and Artabotrys odoratissimus extract described in preparation claim 1～6 any one, is characterized in that being comprised of following steps:
A, extraction: take kuh-seng particle and Artabotrys odoratissimus particle, mix, by solid-liquid ratio, 1:3～12g/ml adds solvent, regulate pH to 5-7, in 60～100 ℃ of refluxing extraction, filter, obtain extract, wherein, described solvent is methyl alcohol, ethanol, ethyl acetate, acetone, chloroform or water;
B, preparation: extract is made to microemulsion, soluble concentrate or aqua.
8. preparation according to claim 7, containing the method for the composition pesticide of kuh-seng and Artabotrys odoratissimus extract, is characterized in that: in described a step, kuh-seng particle and Artabotrys odoratissimus particle are crossed 60～100 mesh sieves preferred mistake 80 mesh sieves; Solid-liquid ratio is 1:4～10g/ml, is preferably 1:6g/ml; PH value is 6.0; Solvent is water; Reflow's cycle is twice, and merging filtrate, obtains extract.
9. the application of the composition pesticide containing kuh-seng and Artabotrys odoratissimus extract described in claim 1～6 any one in control crop pest.
10. the application of the composition pesticide containing kuh-seng and Artabotrys odoratissimus extract according to claim 9 in control crop pest, is characterized in that: described insect pest is Homoptera, Acarina, thrips, lepidoptera pest; During application, be diluted with water to that to take the activity component concentration of crude drug weighing scale be 10～100ppm, be preferably diluted with water to that to take the activity component concentration of crude drug weighing scale be 20～50ppm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310744060.XA CN103651596B (en) | 2013-12-30 | 2013-12-30 | Containing composition pesticide and the preparation method and application of kuh-seng and Artabotrys odoratissimus extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310744060.XA CN103651596B (en) | 2013-12-30 | 2013-12-30 | Containing composition pesticide and the preparation method and application of kuh-seng and Artabotrys odoratissimus extract |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103651596A true CN103651596A (en) | 2014-03-26 |
| CN103651596B CN103651596B (en) | 2016-01-20 |
Family
ID=50291313
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310744060.XA Active CN103651596B (en) | 2013-12-30 | 2013-12-30 | Containing composition pesticide and the preparation method and application of kuh-seng and Artabotrys odoratissimus extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103651596B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104829529A (en) * | 2015-05-15 | 2015-08-12 | 海南师范大学 | Artabotrys pilosus total alkaloid extract and application thereof |
| CN114651831A (en) * | 2022-04-06 | 2022-06-24 | 山东百士威作物保护有限公司 | Preparation method of biological pesticide |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101720791A (en) * | 2008-10-24 | 2010-06-09 | 沈阳东大迪克化工药业有限公司 | Matrine pure plant source pesticide |
| KR20130138440A (en) * | 2012-06-11 | 2013-12-19 | 경남과학기술대학교 산학협력단 | Pesticidal composition comprising neem extract and sophora flavescens aiton extract as effective components and pesticidal method using thereof |
-
2013
- 2013-12-30 CN CN201310744060.XA patent/CN103651596B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101720791A (en) * | 2008-10-24 | 2010-06-09 | 沈阳东大迪克化工药业有限公司 | Matrine pure plant source pesticide |
| KR20130138440A (en) * | 2012-06-11 | 2013-12-19 | 경남과학기술대학교 산학협력단 | Pesticidal composition comprising neem extract and sophora flavescens aiton extract as effective components and pesticidal method using thereof |
Non-Patent Citations (5)
| Title |
|---|
| 中国土农药志编辑委员会: "《中国土农药志》", 31 May 1959 * |
| 徐今宁 等: "苦参碱提取工艺及多种制剂的研究进展", 《时珍国医国药》 * |
| 章新军 等: "植物源农药防治烟草病虫害", 《烟草科技》 * |
| 苟亚峰 等: "12种热带植物粗提物对胡椒瘟病的抑菌活性测定", 《热带农业科学》 * |
| 陈振德 等: "鹰爪花属植物化学成分研究进展", 《国外医药(植物药分册)》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104829529A (en) * | 2015-05-15 | 2015-08-12 | 海南师范大学 | Artabotrys pilosus total alkaloid extract and application thereof |
| CN104829529B (en) * | 2015-05-15 | 2017-03-22 | 海南师范大学 | Artabotrys pilosus total alkaloid extract and application thereof |
| CN114651831A (en) * | 2022-04-06 | 2022-06-24 | 山东百士威作物保护有限公司 | Preparation method of biological pesticide |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103651596B (en) | 2016-01-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103651632B (en) | Containing the composition pesticide and its preparation method and application of Fructus meliae toosendan and black false hellebore extract | |
| CN103704281B (en) | Containing the composition pesticide and its preparation method and application of kuh-seng and tobacco extract | |
| CN103651608B (en) | Containing composition pesticide and the preparation method and application of kitchen garden and aconite extract | |
| CN103651605B (en) | Containing composition pesticide and the preparation method and application of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract | |
| CN103704278B (en) | Containing composition pesticide and the preparation method and application of Fructus meliae toosendan and triperygium wilfordii extractive | |
| CN103704284B (en) | Containing composition pesticide and the preparation method and application of black false hellebore and rhizoma calami extract | |
| CN103651596B (en) | Containing composition pesticide and the preparation method and application of kuh-seng and Artabotrys odoratissimus extract | |
| CN103636683B (en) | Pesticide composition containing radix sophorae flavescentis and fructus cnidii extracts as well as preparation method and application of pesticide composition | |
| CN103734204B (en) | Pesticide composite containing schizonepeta and tobacco extract, preparation method and application | |
| CN105284914A (en) | Pesticide composition containing cynanchum komarovii and sophora alopecuroide extract as well as preparation method and application thereof | |
| CN103689002B (en) | Containing composition pesticide and the preparation method and application of lantana and Azolla imbricata extract | |
| CN103651592B (en) | Containing composition pesticide and the preparation method and application of Dalmatian chrysanthemum and extract from Lantana camara | |
| CN103651597B (en) | Containing the agricultural chemicals and its production and use of acalypha copperleaf and Derris ellipta extract | |
| CN103651595B (en) | Pesticide composition containing lightyellow sophora root and Caesalpinia crista extracts, and preparation method and application thereof | |
| CN103651633B (en) | Pesticide composition containing lightyellow sophora root and veratrum nigrum extracts, and preparation method and application thereof | |
| CN105284913A (en) | Pesticide composition containing sophora alopecuroide and vetchleaf sophora root extract as well as preparation method and application thereof | |
| CN105123700A (en) | Insecticidal and bactericidal agent with thymol and arecoline and preparation method of insecticidal and bactericidal agent | |
| CN103704285B (en) | Pesticide composition containing extracts of veratrum nigrum and mamee-apple and preparation method and application of pesticide combination | |
| CN103651598B (en) | Containing the agricultural chemicals and its production and use of Annona reticulata and Derris ellipta extract | |
| CN105340979A (en) | Pesticide composition containing chenopodium ambrosioides and common peganum seed extract as well as preparation method and application of pesticide composition | |
| CN102630676A (en) | Efficient insecticidal composition | |
| CN103651616B (en) | Containing the agricultural chemicals and its production and use of potato and Derris ellipta extract | |
| CN105284915A (en) | Pesticide composition containing extracts of folium isatidis and thyme and preparation method and application thereof | |
| CN105284967A (en) | Pesticide composition containing extracts of cymbopogon distans and raw gingers and preparation method and application thereof | |
| CN103766411B (en) | Containing composition pesticide and the preparation method and application of chrysanthemum wormwood artemisia and pyrethrum extract |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 611630 No. five, No. 35 Industrial Road, Heshan Town, Pujiang County, Sichuan, Chengdu Patentee after: Chengdu new Chaoyang Crop Science Co., Ltd Address before: 611630 No. five, No. 35 Industrial Road, Heshan Town, Pujiang County, Sichuan, Chengdu Patentee before: CHENGDU NEWSUN CROPSCIENCE Co.,Ltd. |