CN103651605A - Pesticide composition containing szechwan chinaberry fruit and lettuce extracts, and preparation method and application thereof - Google Patents
Pesticide composition containing szechwan chinaberry fruit and lettuce extracts, and preparation method and application thereof Download PDFInfo
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- CN103651605A CN103651605A CN201310745492.2A CN201310745492A CN103651605A CN 103651605 A CN103651605 A CN 103651605A CN 201310745492 A CN201310745492 A CN 201310745492A CN 103651605 A CN103651605 A CN 103651605A
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Abstract
The invention relates to a pesticide composition containing szechwan chinaberry fruit and lettuce extracts, and a preparation method and application thereof, and belongs to the field of pesticides. In order to solve technical problems, the invention provides the pesticide composition containing the szechwan chinaberry fruit and lettuce extracts, and the preparation method and application thereof. According to the pesticide composition containing the szechwan chinaberry fruit and lettuce extracts, active ingredients of the pesticide composition are extracted from 1 weight part of szechwan chinaberry fruit and 0.5 to 8 weight parts of lettuce; the active ingredients can be prepared by mixing the szechwan chinaberry fruit and lettuce and extracting together, or separately extracting the szechwan chinaberry fruit to obtain the szechwan chinaberry fruit extract, separately extracting the lettuce to obtain the lettuce extract, and mixing the two extracts. The pesticide composition containing the szechwan chinaberry fruit and lettuce extracts can well kill Lepidoptera pests, particularly Pyralidae, Pierididae and Noctuidae insects such as Hymenia recurvalis Fabricius, pieris rapae and cotton bollworm, and has positive significance for agricultural production.
Description
Technical field
The present invention relates to composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract and its preparation method and application, belong to pesticide field.
Background technology
Fructus meliae toosendan has another name called FRUCTUS TOOSENDAN, and traditional Chinese medicine is the ripening fruits of Meliaceae deciduous tree melia toosendan tree.Mainly originate in Chinese southern various places, superior with the product person in Sichuan, therefore have another name called Fructus meliae toosendan.Fructus meliae toosendan is cold in nature, and bitter is a kind of of qi-activating drug.Mainly enter Liver Channel, catharsis liver heat, promoting qi circulation and relieving pain, has the function except damp and hot, clearing away the liver-fire, pain relieving, desinsection, is the efficient important source material without the free of contamination novel plant class of residual hazard agricultural chemicals of preparation.
Lettuce is the annual or biennial vegetable of composite family Lactuca, and it is a kind of very common edible vegetable.In lettuce, the content of carbohydrate is lower, and mineral salt, vitamin content are abundanter, especially contains more nicotinic acid.In lettuce, also contain a certain amount of trace element zinc, iron, the potassium content in lettuce is abundant, is 27 times of sodium salt content.The nutrient component of lettuce is a lot, comprises protein, fat, carbohydrate, ash content, provitamin A, vitamin B1, vitamin B2, vitamin c, microelements of calcium, phosphorus, iron, potassium, magnesium, silicon etc. and food fiber etc.Every 100 grams of lettuces are got over 96.4 grams of moisture contents, 0.6 gram, protein, 0.1 gram, fat, 1.9 grams, carbohydrate, 0.4 gram of raw fiber, 7 milligrams of calcium, 31 milligrams, phosphorus, 2 milligrams of iron, 0.02 milligram of carotin, 0.03 milligram of thiamine, 0.02 milligram, vitamin b3,0.5 milligram of niacin.
Inventor finds through a large amount of experiments, the common extract of Fructus meliae toosendan and lettuce or Sichuan chinaberry extract and Caulis et Folium Lactucae sativae extract are mixed, active insecticidal components wherein has synergistic function, can be good at killing lepidoptera pest, especially Pyralidae, Sulfur butterfly, noctuid insect, as beet snout moth's larva, cabbage caterpillar, cotton bollworm etc., for agricultural production, there is positive effect.
Summary of the invention
The technical problem to be solved in the present invention is to provide containing composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract and its preparation method and application.
The present invention is containing the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, and its active component is formed by the crude drug extraction of following weight portion: 1 part of Fructus meliae toosendan, 0.5～8 part, lettuce.Wherein, active component is preferably formed by the crude drug extraction of following weight portion: 1 part of Fructus meliae toosendan, 2～5 parts, lettuce; The crude drug extraction being preferably again by following weight portion forms: 1 part of Fructus meliae toosendan, 3～4 parts, lettuce; The more preferably crude drug extraction by following weight portion forms: 1 part of Fructus meliae toosendan, 4 parts, lettuce.
Wherein, Fructus meliae toosendan and lettuce that the present invention adopts are Chinese herbal medicine, and in order to improve the present invention containing the activity of the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, Fructus meliae toosendan is dry Fructus meliae toosendan preferably, and lettuce is dry acrial parts such as lettuce haulm preferably.
The present invention is containing the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, and after its active component can be mixed by crude drug Fructus meliae toosendan and lettuce, common extraction forms; Also can first Fructus meliae toosendan be extracted and obtain Sichuan chinaberry extract separately, lettuce be extracted and obtains Caulis et Folium Lactucae sativae extract separately, then two kinds of extracts are mixed.In order to improve the insecticidal properties of composition pesticide of the present invention, after its active component is preferably and is mixed by Fructus meliae toosendan and lettuce, common extraction forms.
Further, in order to improve the stability containing the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, in composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract of the present invention, also comprise auxiliary agent, described auxiliary agent can adopt the usual auxiliaries in agricultural chemicals, as at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
Wherein, emulsifier can adopt at least one in non-ionic surface active agent and anion surfactant, described non-ionic surface active agent can be dibenzyl xenyl APEO, styryl phenyl APEO, styryl phenyl APEO, phenethyl phenyl polyoxyethylene one polyethenoxy ether, OPEO, alkylphenol polyoxyethylene sulfosuccinate, aliphatic acid polyethenoxy ether, fatty alcohol-polyoxyethylene ether, aliphatic amine polyoxyethylene ether, castor oil ethylene oxide adduct and derivative thereof, at least one in polyol fatty acid ester and ethylene oxide adduct etc. thereof, described anion surfactant is at least one in calcium dodecyl benzene sulfonate, alkylphenol polyoxyethylene phosphate, phenethyl phenol polyethenoxy ether phosphate etc., as emulsifier 500,601p, 602p etc., in addition, can also use commercially available emulsifier production.
Wherein, solvent can adopt at least one in water, ethanol, isopropyl alcohol, n-butanol, ethyl acetate, butyl acetate, methyl-sulfoxide, turpentine oil, solvent naphtha, 1-METHYLPYRROLIDONE, dimethyl formamide etc.
Wherein, antifreeze can be glycerine, propane diols, ethylene glycol, sodium chloride, calcium chloride, sorbierite etc.
Wherein, defoamer is silicone defoaming agent etc.
Wherein, dispersant is at least one in lignosulfonates, PAA and propionamide copolymer, alkylnaphthalene sulfonate, polycarboxylic acid salt's condensation product, methyl sodiosul foaliphatate etc.
Wherein, wetting agent can be alkyl sulfate, alkylbenzenesulfonate, alkylnaphthalene sulfonate, aliphatic alcohol polyethenoxy base ether, alkylphenol-polyethenoxy base ether, sorbitan fatty acid ester etc.
Wherein, stabilizing agent can be 2-expoxy propane, butyl glycidyl ether, isopropyl alcohol, n-butanol, polyethylene glycol etc.
Composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract of the present invention can be prepared into by the method for this area routine any one formulation that is applicable to agricultural use, for the ease of storing and transportation, can make microemulsion, soluble concentrate or aqua.
Further, be preferably the aqua that the component by following weight portion forms: Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, 602,5 parts of ethylene glycol of 8 parts of emulsifier, 47 parts of water; Wherein, Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract are that the crude drug of 40 parts extracts and to form.Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract can obtain for Fructus meliae toosendan and lettuce mix rear common extraction in proportion; Also can take in proportion Fructus meliae toosendan and lettuce, after extracting separately, be mixed to get.
For the ease of storing and transportation, can extract is concentrated, obtain concentrate, and then be prepared into various formulations, cycles of concentration can be adjusted voluntarily according to needs of production, as preferred version, Fructus meliae toosendan is prepared from by the following method with Caulis et Folium Lactucae sativae extract: crude drug is identical with crude drug weight through extracting and being concentrated into.
Further, the invention also discloses the present invention containing the preparation method of the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, it is comprised of following steps:
A, extraction: take Fructus meliae toosendan particle and lettuce particle, mix, by solid-liquid ratio, 1:3～12g/ml adds solvent, in 60～95 ℃ of refluxing extraction, filter, and obtains extract, and wherein, described solvent is organic solvent or water;
B, preparation: extract is made to microemulsion, soluble concentrate or aqua.
Wherein, Fructus meliae toosendan particle and lettuce particle can adopt commercially available particle, also can adopt conventional method to pulverize the acrial parts such as Fructus meliae toosendan, lettuce haulm and obtain.In order to improve the effect of extraction, simultaneously cost-saving, Fructus meliae toosendan particle and lettuce particle are crossed 60～100 mesh sieves, preferred mistake 80 mesh sieves.
Further, for cost-saving, simultaneously also in order to improve extraction efficiency, the solid-liquid ratio in a step is preferably 1:4～10g/ml, more preferably 1:6g/ml.The Fructus meliae toosendan and the lettuce particle that are lg can add 3～12ml solvent extraction, preferably add 4～10ml, more preferably add 6ml.
Conventional organic solvent all can be used for the present invention, for cost-saving, improves dissolution rate, guarantees extraction efficiency simultaneously, and organic solvent is preferably methyl alcohol, ethanol, ethyl acetate, acetone, chloroform, more preferably 95%v/v ethanol.
In order to shorten preparation time, accelerate extraction rate, can when adding hot reflux, stir, mixing speed is preferably 80～100 revs/min.
Further, reflux temperature is preferably 75～85 ℃, more preferably 82 ℃; Reflow's cycle is twice, and each return time is 1.5 hours, and merging filtrate, obtains extract.
The present invention also provides the application of the composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract of the present invention in control crop pest.Composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract of the present invention, to lepidoptera pest, especially Pyralidae, Sulfur butterfly, noctuid insect, as beet snout moth's larva, cabbage caterpillar, cotton bollworm etc. have good preventive effect.
In actual agricultural production, in order to improve composition pesticide containing Fructus meliae toosendan and the Caulis et Folium Lactucae sativae extract control efficiency to insect pest, the described composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract is preferably applied to crop pest initial stage of origination; Composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract of the present invention can be used by common method, sprays on crop blade face.As preferred version, while using, the present invention is diluted with water to containing the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract to take the activity component concentration of crude drug weighing scale be 10～100ppm, it is the composition pesticide making after 1g crude drug extracts, when using, be diluted with water to 10kg, its activity component concentration equals 100ppm; Further preferably being diluted to activity component concentration is that 20～50ppm sprays in plant blade face, and to occur water membrane on blade face, not lower of liquid is advisable; After being preferable over 3～5 days, carry out secondary dispenser, the preferred continuous administration of a season crop 2～3 times.
Composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract of the present invention is mixed by Sichuan chinaberry extract and Caulis et Folium Lactucae sativae extract, or formed by Fructus meliae toosendan and common extraction of lettuce, its preventive effect is far away higher than the extract of single component, particularly Fructus meliae toosendan and lettuce are mixed to rear common extraction, owing to having solubilising, hydrotropy effect between Fructus meliae toosendan and lettuce, the composition pesticide insecticidal effect of making after common extraction is more remarkable.
Compared with prior art, the composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract of the present invention has the following advantages:
1, the composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract of the present invention has synergistic function, obvious to the control efficiency of lepidoptera pest.
2, Fructus meliae toosendan of the present invention and lettuce are common extracts or extracts respectively rear mixing, and the composition pesticide of making can improve crop immune system ability in quick-acting tagging, and can significantly improve its vigor.
3, the present invention be take Chinese herbal medicine as raw material, thereby does not have Pesticide Residue, and insect is difficult to produce resistance; Compare with independent use chemical source agricultural chemicals, environmentally friendly.
When 4, the composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract of the present invention is used, valid density is low, with low cost.
Embodiment
The present invention is containing the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, and its active component is formed by the crude drug extraction of following weight portion: 1 part of Fructus meliae toosendan, 0.5～8 part, lettuce.Wherein, active component is preferably formed by the crude drug extraction of following weight portion: 1 part of Fructus meliae toosendan, 2～5 parts, lettuce; The crude drug extraction being preferably again by following weight portion forms: 1 part of Fructus meliae toosendan, 3～4 parts, lettuce; The more preferably crude drug extraction by following weight portion forms: 1 part of Fructus meliae toosendan, 4 parts, lettuce.
Wherein, Fructus meliae toosendan and lettuce that the present invention adopts are Chinese herbal medicine, and in order to improve the present invention containing the activity of the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, Fructus meliae toosendan is dry Fructus meliae toosendan preferably, and lettuce is dry acrial parts such as lettuce haulm preferably.
The present invention is containing the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, and after its active component can be mixed by crude drug Fructus meliae toosendan and lettuce, common extraction forms; Also can first Fructus meliae toosendan be extracted and obtain Sichuan chinaberry extract separately, lettuce be extracted and obtains Caulis et Folium Lactucae sativae extract separately, then two kinds of extracts are mixed.In order to improve the insecticidal properties of composition pesticide of the present invention, after its active component is preferably and is mixed by Fructus meliae toosendan and lettuce, common extraction forms.
Further, in order to improve the stability containing the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, in composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract of the present invention, also comprise auxiliary agent, described auxiliary agent can adopt the usual auxiliaries in agricultural chemicals, as at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
Wherein, emulsifier can adopt at least one in non-ionic surface active agent and anion surfactant, described non-ionic surface active agent can be dibenzyl xenyl APEO, styryl phenyl APEO, styryl phenyl APEO, phenethyl phenyl polyoxyethylene one polyethenoxy ether, OPEO, alkylphenol polyoxyethylene sulfosuccinate, aliphatic acid polyethenoxy ether, fatty alcohol-polyoxyethylene ether, aliphatic amine polyoxyethylene ether, castor oil ethylene oxide adduct and derivative thereof, at least one in polyol fatty acid ester and ethylene oxide adduct etc. thereof, described anion surfactant is at least one in calcium dodecyl benzene sulfonate, alkylphenol polyoxyethylene phosphate, phenethyl phenol polyethenoxy ether phosphate etc., as emulsifier 500,601p, 602p etc., in addition, can also use commercially available emulsifier production.
Wherein, solvent can adopt at least one in water, ethanol, isopropyl alcohol, n-butanol, ethyl acetate, butyl acetate, methyl-sulfoxide, turpentine oil, solvent naphtha, 1-METHYLPYRROLIDONE, dimethyl formamide etc.
Wherein, antifreeze can be glycerine, propane diols, ethylene glycol, sodium chloride, calcium chloride, sorbierite etc.
Wherein, defoamer is silicone defoaming agent etc.
Wherein, dispersant is at least one in lignosulfonates, PAA and propionamide copolymer, alkylnaphthalene sulfonate, polycarboxylic acid salt's condensation product, methyl sodiosul foaliphatate etc.
Wherein, wetting agent can be alkyl sulfate, alkylbenzenesulfonate, alkylnaphthalene sulfonate, aliphatic alcohol polyethenoxy base ether, alkylphenol-polyethenoxy base ether, sorbitan fatty acid ester etc.
Wherein, stabilizing agent can be 2-expoxy propane, butyl glycidyl ether, isopropyl alcohol, n-butanol, polyethylene glycol etc.
Composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract of the present invention can be prepared into by the method for this area routine any one formulation that is applicable to agricultural use, for the ease of storing and transportation, can make microemulsion, soluble concentrate or aqua.
Further, be preferably the aqua that the component by following weight portion forms: Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, 602,5 parts of ethylene glycol of 8 parts of emulsifier, 47 parts of water; Wherein, Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract are that the crude drug of 40 parts extracts and to form.Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract can obtain for Fructus meliae toosendan and lettuce mix rear common extraction in proportion; Also can take in proportion Fructus meliae toosendan and lettuce, after extracting separately, be mixed to get.
For the ease of storing and transportation, can extract is concentrated, obtain concentrate, and then be prepared into various formulations, cycles of concentration can be adjusted voluntarily according to needs of production, as preferred version, Fructus meliae toosendan is prepared from by the following method with Caulis et Folium Lactucae sativae extract: crude drug is identical with crude drug weight through extracting and being concentrated into.
Further, the invention also discloses the present invention containing the preparation method of the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, it is comprised of following steps:
A, extraction: take Fructus meliae toosendan particle and lettuce particle, mix, by solid-liquid ratio, 1:3～12g/ml adds solvent, in 60～95 ℃ of refluxing extraction, filter, and obtains extract, and wherein, described solvent is organic solvent or water;
B, preparation: extract is made to microemulsion, soluble concentrate or aqua.
Wherein, Fructus meliae toosendan particle and lettuce particle can adopt commercially available particle, also can adopt conventional method to pulverize the acrial parts such as Fructus meliae toosendan, lettuce haulm and obtain.In order to improve the effect of extraction, simultaneously cost-saving, Fructus meliae toosendan particle and lettuce particle are crossed 60～100 mesh sieves, preferred mistake 80 mesh sieves.
Further, for cost-saving, simultaneously also in order to improve extraction efficiency, the solid-liquid ratio in a step is preferably 1:4～10g/ml, more preferably 1:6g/ml.The Fructus meliae toosendan and the lettuce particle that are lg can add 3～12ml solvent extraction, preferably add 4～10ml, more preferably add 6ml.
Conventional organic solvent all can be used for the present invention, for cost-saving, improves dissolution rate, guarantees extraction efficiency simultaneously, and organic solvent is preferably methyl alcohol, ethanol, ethyl acetate, acetone, chloroform, more preferably 95%v/v ethanol.
In order to shorten preparation time, accelerate extraction rate, can when adding hot reflux, stir, mixing speed is preferably 80～100 revs/min.
Further, reflux temperature is preferably 75～85 ℃, more preferably 82 ℃; Reflow's cycle is twice, and each return time is 1.5 hours, and merging filtrate, obtains extract.
The present invention also provides the application of the composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract of the present invention in control crop pest.Composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract of the present invention, to lepidoptera pest, especially Pyralidae, Sulfur butterfly, noctuid insect, as beet snout moth's larva, cabbage caterpillar, cotton bollworm etc. have good preventive effect.
In actual agricultural production, in order to improve composition pesticide containing Fructus meliae toosendan and the Caulis et Folium Lactucae sativae extract control efficiency to insect pest, the described composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract is preferably applied to crop pest initial stage of origination; Composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract of the present invention can be used by common method, sprays on crop blade face.As preferred version, while using, the present invention is diluted with water to containing the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract to take the activity component concentration of crude drug weighing scale be 10～100ppm, it is the composition pesticide making after 1g crude drug extracts, when using, be diluted with water to 10kg, its activity component concentration equals 100ppm; Further preferably being diluted to activity component concentration is that 20～50ppm sprays in plant blade face, and to occur water membrane on blade face, not lower of liquid is advisable; After being preferable over 3～5 days, carry out secondary dispenser, the preferred continuous administration of a season crop 2～3 times.
Composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract of the present invention is mixed by Sichuan chinaberry extract and Caulis et Folium Lactucae sativae extract, or formed by Fructus meliae toosendan and common extraction of lettuce, its preventive effect is far away higher than the extract of single component, particularly Fructus meliae toosendan and lettuce are mixed to rear common extraction, owing to having solubilising, hydrotropy effect between Fructus meliae toosendan and lettuce, the composition pesticide insecticidal effect of making after common extraction is more remarkable.
Below in conjunction with embodiment, the specific embodiment of the present invention is further described, does not therefore limit the present invention among described scope of embodiments.
Embodiment 1 is containing the preparation of the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract
A, extraction: take dry Fructus meliae toosendan and lettuce haulm, mix, pulverize, cross 80 mesh sieves, add extraction solution, refluxing extraction twice, filters, and merging filtrate is concentrated, makes extract weight equal the weight of crude drug, obtains extract cy-01～cy-06; Wherein, each parameter is in Table 1;
B, preparation: auxiliary agent add table 2 in extract cy-01～cy-06 in, stir, must be containing the composition pesticide CY-01～CY-06 of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract.
The preparation technology parameter of table 1 Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract
Continued 1
| cy-03 | 1 | 4 | 1:6 | 95%v/v ethanol | 82 | 1.5 |
| cy-04 | 1 | 3 | 1:3 | Water | 95 | 3 |
| cy-05 | 1 | 5 | 1:8 | Methyl alcohol | 75 | 2 |
| cy-06 | 1 | 8 | 1:4 | Chloroform | 60 | 1 |
Wherein, Fructus meliae toosendan and lettuce haulm are all purchased from Anguo City Zhong Tian prepared slices of Chinese crude drugs Co., Ltd.
Table 2 is containing the formula of the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract
Embodiment 2 is containing the preparation of the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract
A, extraction: take dry Fructus meliae toosendan, pulverize, cross 80 mesh sieves, add extraction solution, refluxing extraction twice, filters, and merging filtrate is concentrated, makes extract weight equal the weight of crude drug, obtains extract d1-01～d1-03, and wherein, each parameter is in Table 3;
Take dry lettuce haulm, pulverize, cross 80 mesh sieves, add extraction solution, refluxing extraction twice, filters, and merging filtrate is concentrated, makes extract weight equal the weight of crude drug, obtains extract d2-01～d2-03, and wherein, each parameter is in Table 4;
B, preparation: take Fructus meliae toosendan extract, lettuce extract and auxiliary agent, stirring and evenly mixing, obtains the composition pesticide CY-07～CY-12 containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, and wherein, each parameter is in Table 5.
Table 3
Table 4
Table 5
Continued 5
Test example 1 the present invention is containing the efficiency test of the composition pesticide tea tree tea geometrid of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract
1, test material
1.1 for studying thing and object: tea tree tea geometrid
1.2 reagent agent
Product in embodiment: CY-01～CY-12;
Fructus meliae toosendan single dose (D1), lettuce single dose (D2);
Chemical reagent contrast: gamma cyhalothrin (CK-01), purchased from the biochemical Co., Ltd of Chengdu Ke Lilong;
Clear water contrast (CK-02).
Wherein, the preparation method of Fructus meliae toosendan single dose is: get the d1-03 of 40g, add 8g emulsifier 602, and 5g ethylene glycol, 47g water, stirring and evenly mixing, obtains.
The preparation method of lettuce single dose is: gets the d2-03 of 40g, adds 8g emulsifier 602, and 5g ethylene glycol, 47g water, stirring and evenly mixing, obtains.
2, experimental scheme and method
12 medicaments of this test, 2 single dose contrasts, 1 contrast medicament, 1 blank, repeats Gong48Ge community for three times.Minizone and surrounding all leave isolation strip, community area 15m
2, randomized arrangement is respectively processed in community.
By reagent agent thin up, to activity component concentration, be 50ppm, spray application to plant blade face, to occur water membrane on blade face, not lower of liquid is advisable.
Before test, carry out tea geometrid quantity statistics the listing mark of fixed blade.Within 5 days after dispenser, investigate and record the tea geometrid number alive on each blade.Calculate insect population decline rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
3, result of the test
Each group meter that repeats to average, calculates insect population decline rate and preventive effect, and it the results are shown in Table 6.
Table 6
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Insect population decline rate (%) | Preventive effect (%) |
| CY-01 | 489 | 130 | 73.42 | 76.38 |
| CY-02 | 457 | 110 | 75.93 | 78.61 |
| CY-03 | 468 | 81 | 82.69 | 84.62 |
| CY-04 | 424 | 85 | 79.95 | 82.19 |
| CY-05 | 416 | 96 | 76.92 | 79.50 |
| CY-06 | 445 | 114 | 74.38 | 77.24 |
| CY-07 | 486 | 184 | 62.14 | 66.36 |
| CY-08 | 493 | 173 | 64.91 | 68.82 |
| CY-09 | 474 | 137 | 71.10 | 74.32 |
| CY-10 | 465 | 146 | 68.60 | 72.10 |
| CY-11 | 405 | 137 | 66.17 | 69.95 |
| CY-12 | 449 | 154 | 65.70 | 69.53 |
| D1 | 467 | 304 | 34.90 | 42.16 |
| D2 | 481 | 462 | 3.95 | 14.66 |
| CK-01 | 484 | 73 | 84.92 | 86.60 |
| CK-02 | 454 | 511 | -12.56 | —— |
As can be seen from Table 6, product of the present invention has good preventive effect to tea geometrid, and better than the control efficiency of single Sichuan chinaberry extract and Caulis et Folium Lactucae sativae extract, visible, between product compositions of the present invention, has synergistic function.And the preventive effect that adopts the product that common extracting method makes is apparently higher than blended product after independent extraction, visible common extraction contributes to improve dissolution rate, improves insecticide efficiency.In addition, during dispenser, have no crop is had to poisoning performance.
Test example 2 the present invention are containing the efficiency test of the composition pesticide cabbage pieris of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract
Products C Y-01～CY-12 of the present invention is used for preventing and treating cabbage pieris.12 medicaments of this test, 2 single doses, 1 blank, repeats Gong45Ge community for three times.Minizone and surrounding all leave isolation strip, community area 10m
2, randomized arrangement is respectively processed in community.
By reagent agent thin up, to activity component concentration, be 50ppm, spray application to plant blade face, to occur water membrane on blade face, not lower of liquid is advisable.
Before test, carry out cabbage caterpillar quantity statistics alive the listing mark of fixed blade.Within 5 days after dispenser, investigate and record the cabbage caterpillar quantity alive on each blade.Calculate insect population decline rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group meter that repeats to average, calculates insect population decline rate and preventive effect, and it the results are shown in Table 7.
Table 7
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Insect population decline rate (%) | Preventive effect (%) |
| CY-01 | 433 | 95 | 78.06 | 81.50 |
| CY-02 | 526 | 88 | 83.27 | 85.90 |
| CY-03 | 486 | 64 | 86.83 | 88.90 |
| CY-04 | 454 | 68 | 85.02 | 87.37 |
| CY-05 | 419 | 72 | 82.82 | 85.51 |
| CY-06 | 465 | 99 | 78.71 | 82.05 |
| CY-07 | 448 | 158 | 64.73 | 70.27 |
| CY-08 | 432 | 130 | 69.91 | 74.63 |
| CY-09 | 416 | 112 | 73.08 | 77.30 |
| CY-10 | 499 | 139 | 72.14 | 76.52 |
| CY-11 | 501 | 147 | 70.66 | 75.27 |
| CY-12 | 503 | 148 | 70.58 | 75.20 |
| D1 | 528 | 343 | 35.04 | 45.24 |
| D2 | 467 | 448 | 4.07 | 19.13 |
| CK-02 | 553 | 656 | -18.63 | —— |
As can be seen from Table 7, product dialogue cabbage caterpillar of the present invention has good preventive effect, and better than the control efficiency of single Sichuan chinaberry extract and Caulis et Folium Lactucae sativae extract, visible, between product compositions of the present invention, has synergistic function.And the preventive effect that adopts the product that common extracting method makes is apparently higher than blended product after independent extraction, visible common extraction contributes to improve dissolution rate, improves insecticide efficiency.In addition, during dispenser, have no crop is had to poisoning performance.
Test example 3 the present invention are containing the efficiency test of the composition pesticide cucumber beet snout moth's larva of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract
Products C Y-01～CY-12 of the present invention is used for preventing and treating cucumber beet snout moth's larva.12 medicaments of this test, 2 single doses, 1 blank, repeats Gong45Ge community for three times.Minizone and surrounding all leave isolation strip, community area 20m
2, randomized arrangement is respectively processed in community.
By reagent agent thin up, to activity component concentration, be 50ppm, spray application to plant blade face, to occur water membrane on blade face, not lower of liquid is advisable.
Before test, carry out beet snout moth's larva quantity statistics alive the listing mark of fixed blade.Within 5 days after dispenser, investigate and record the beet snout moth's larva number alive on each blade.Calculate insect population decline rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group meter that repeats to average, calculates insect population decline rate and preventive effect, and its result is as shown in table 8.
Table 8
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Insect population decline rate (%) | Preventive effect (%) |
| CY-01 | 264 | 56 | 78.79 | 81.92 |
| CY-02 | 199 | 30 | 84.92 | 87.15 |
| CY-03 | 206 | 24 | 88.35 | 90.07 |
| CY-04 | 245 | 35 | 85.71 | 87.82 |
| CY-05 | 222 | 38 | 82.88 | 85.41 |
| CY-06 | 216 | 41 | 81.02 | 83.82 |
| CY-07 | 235 | 74 | 68.51 | 73.16 |
| CY-08 | 264 | 66 | 75.00 | 78.69 |
| CY-09 | 259 | 60 | 76.83 | 80.25 |
| CY-10 | 249 | 64 | 74.30 | 78.09 |
| CY-11 | 275 | 74 | 73.09 | 77.06 |
| CY-12 | 255 | 73 | 71.37 | 75.60 |
| D1 | 246 | 163 | 33.74 | 43.52 |
| D2 | 215 | 205 | 4.65 | 18.72 |
| CK-02 | 208 | 244 | -17.31 | —— |
As can be seen from Table 8, product of the present invention has good preventive effect to beet snout moth's larva, and better than the control efficiency of single Sichuan chinaberry extract and Caulis et Folium Lactucae sativae extract, visible, between product compositions of the present invention, has synergistic function.And the preventive effect that adopts the product that common extracting method makes is apparently higher than blended product after independent extraction, visible common extraction contributes to improve dissolution rate, improves insecticide efficiency.In addition, during dispenser, have no crop is had to poisoning performance.
Test example 4 the present invention are containing the efficiency test of the composition pesticide a variety of Chinese cabbage diamond-back moth of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract
Products C Y-01～CY-12 of the present invention is used for preventing and treating a variety of Chinese cabbage diamond-back moth.12 medicaments of this test, 2 single doses, 1 blank, repeats Gong45Ge community for three times.Minizone and surrounding all leave isolation strip, community area 10m
2, randomized arrangement is respectively processed in community.
By reagent agent thin up, to activity component concentration, be 50ppm, spray application to plant blade face, to occur water membrane on blade face, not lower of liquid is advisable.
Before test, carry out diamond-back moth quantity statistics alive the listing mark of fixed blade.Within 5 days after dispenser, investigate and record the diamond-back moth number alive on each blade.Calculate insect population decline rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group meter that repeats to average, calculates insect population decline rate and preventive effect, and its result is as shown in table 9.
Table 9
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Insect population decline rate (%) | Preventive effect (%) |
| CY-01 | 193 | 38 | 80.31 | 83.10 |
| CY-02 | 216 | 32 | 85.19 | 87.28 |
| CY-03 | 206 | 26 | 87.38 | 89.17 |
| CY-04 | 253 | 38 | 84.98 | 87.11 |
| CY-05 | 205 | 35 | 82.93 | 85.34 |
| CY-06 | 215 | 41 | 80.93 | 83.63 |
| CY-07 | 203 | 58 | 71.43 | 75.47 |
| CY-08 | 195 | 48 | 75.38 | 78.87 |
| CY-09 | 186 | 43 | 76.88 | 80.16 |
| CY-10 | 215 | 51 | 76.28 | 79.64 |
| CY-11 | 230 | 56 | 75.65 | 79.10 |
| CY-12 | 222 | 58 | 73.87 | 77.57 |
Continued 9
| D1 | 189 | 116 | 38.62 | 47.31 |
| D2 | 254 | 244 | 3.94 | 17.54 |
| CK-02 | 194 | 226 | -16.49 | —— |
As can be seen from Table 9, product of the present invention has good preventive effect to diamond-back moth, and better than the control efficiency of single Sichuan chinaberry extract and Caulis et Folium Lactucae sativae extract, visible, between product compositions of the present invention, has synergistic function.And the preventive effect that adopts the product that common extracting method makes is apparently higher than blended product after independent extraction, visible common extraction contributes to improve dissolution rate, improves insecticide efficiency.In addition, during dispenser, have no crop is had to poisoning performance.
Claims (10)
1. containing the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, it is characterized in that, its active component is formed by the crude drug extraction of following weight portion: 1 part of Fructus meliae toosendan, 0.5～8 part, lettuce.
2. the composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract according to claim 1, is characterized in that, its active component is formed by the crude drug extraction of following weight portion: 1 part of Fructus meliae toosendan, 2～5 parts, lettuce; Be preferably 1 part of Fructus meliae toosendan, 3～4 parts, lettuce; More preferably Fructus meliae toosendan is 1 part, 4 parts, lettuce.
3. the composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract according to claim 1 and 2, is characterized in that: after its active component is mixed by Fructus meliae toosendan and lettuce, common extraction forms; Or by Fructus meliae toosendan, extract the Sichuan chinaberry extract, the lettuce that obtain and extract the Caulis et Folium Lactucae sativae extract obtaining and mix.
4. according to the composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract described in claim 1～3 any one, it is characterized in that: the described composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract is microemulsion, soluble concentrate or aqua.
5. according to the composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract described in claim 1～4 any one, it is characterized in that: in the described composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, also comprise auxiliary agent, described auxiliary agent is at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
6. the composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract according to claim 5, is characterized in that: described aqua is comprised of the component of following weight portion: Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, 602,5 parts of ethylene glycol of 8 parts of emulsifier, 47 parts of water; Wherein, Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract are that the crude drug of 40 parts extracts and to form; Fructus meliae toosendan is preferably prepared from by the following method with Caulis et Folium Lactucae sativae extract: crude drug is identical with crude drug weight through extracting and being concentrated into.
7. the method for the composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract described in preparation claim 1～6 any one, is characterized in that being comprised of following steps:
A, extraction: take Fructus meliae toosendan particle and lettuce particle, mix, by solid-liquid ratio, 1:3～12g/ml adds solvent, in 60～95 ℃ of refluxing extraction, filter, and obtains extract, and wherein, described solvent is organic solvent or water;
B, preparation: extract is made to microemulsion, aqueous emulsion, soluble concentrate or aqua.
8. preparation according to claim 7, containing the method for the composition pesticide of Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract, is characterized in that: in described a step, Fructus meliae toosendan particle and lettuce particle are crossed 60～100 mesh sieves preferred mistake 80 mesh sieves; Solid-liquid ratio is 1:4～10g/ml, is preferably 1:6g/ml; Organic solvent is methyl alcohol, ethanol, ethyl acetate, acetone, chloroform, is preferably 95%v/v ethanol; Reflux temperature, for being 75～85 ℃, is preferably 82 ℃; Reflow's cycle is twice, and merging filtrate, obtains extract.
9. the application of the composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract described in claim 1～6 any one in control crop pest.
10. the application of the composition pesticide containing Fructus meliae toosendan and Caulis et Folium Lactucae sativae extract according to claim 9 in control crop pest, is characterized in that: described insect pest is lepidoptera pest; During application, be diluted with water to that to take the activity component concentration of crude drug weighing scale be 10～100ppm, be preferably diluted with water to that to take the activity component concentration of crude drug weighing scale be 20～50ppm.
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| CN106490017A (en) * | 2016-10-23 | 2017-03-15 | 张颂忠 | A kind of foliage fertilizer plant pesticide of preventing and treating tetranychus viennensis |
| CN106508987A (en) * | 2016-10-21 | 2017-03-22 | 赖丰光 | Pesticide fertilizer for controlling diamondback moth |
| CN107593781A (en) * | 2017-10-27 | 2018-01-19 | 张颂忠 | A kind of composition pesticide containing Caulis et Folium Lactucae sativae extract and methoxyfenozide |
| CN107624802A (en) * | 2017-10-27 | 2018-01-26 | 惠州市无龄康态健康科技有限公司 | A kind of composition pesticide containing Caulis et Folium Lactucae sativae extract and bromine cyanogen insect amide |
| CN107646883A (en) * | 2017-10-27 | 2018-02-02 | 张锡林 | A kind of composition pesticide containing Caulis et Folium Lactucae sativae extract and fluoroform pyrrole ether |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106508987A (en) * | 2016-10-21 | 2017-03-22 | 赖丰光 | Pesticide fertilizer for controlling diamondback moth |
| CN106490017A (en) * | 2016-10-23 | 2017-03-15 | 张颂忠 | A kind of foliage fertilizer plant pesticide of preventing and treating tetranychus viennensis |
| CN107593781A (en) * | 2017-10-27 | 2018-01-19 | 张颂忠 | A kind of composition pesticide containing Caulis et Folium Lactucae sativae extract and methoxyfenozide |
| CN107624802A (en) * | 2017-10-27 | 2018-01-26 | 惠州市无龄康态健康科技有限公司 | A kind of composition pesticide containing Caulis et Folium Lactucae sativae extract and bromine cyanogen insect amide |
| CN107646883A (en) * | 2017-10-27 | 2018-02-02 | 张锡林 | A kind of composition pesticide containing Caulis et Folium Lactucae sativae extract and fluoroform pyrrole ether |
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| CN103651605B (en) | 2016-04-27 |
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