CN103651608A - Pesticide composition containing parslane herb and common monkshood mother root extracts, and preparation method and application thereof - Google Patents
Pesticide composition containing parslane herb and common monkshood mother root extracts, and preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to a pesticide composition containing parslane herb and common monkshood mother root extracts, and a preparation method and application thereof, and belongs to the field of pesticides. In order to solve technical problems, the invention provides the pesticide composition containing the parslane herb and common monkshood mother root extracts, and the preparation method and application thereof. According to the pesticide composition containing the parslane herb and common monkshood mother root extracts, active ingredients of the pesticide composition are extracted from 1 weight part of parslane herb and 0.2 to 4 weight parts of common monkshood mother root; the active ingredients are prepared by mixing the parslane herb and the common monkshood mother root and extracting together, or prepared by separately extracting the parslane herb to obtain the parslane herb extract, separately extracting the common monkshood mother root to obtain the common monkshood mother root extract, and mixing the two extracts. The pesticide composition containing the parslane herb and common monkshood mother root extracts has an obvious effect of controlling Lepidoptera pests, and has positive significance for agricultural production.
Description
Technical field
The present invention relates to composition pesticide and preparation method and application containing kitchen garden and aconite extract, belong to pesticide field.
Background technology
In recent years, in agriculture and forestry field, due to single drug and Scientific Usage of Drugs not, plant pest has produced pesticide resistance in various degree, and agriculture and forestry are produced still with chemical pesticide as mainly preventing and treating kind, have caused the destruction increasingly of environmental pollution and ecotope.Along with the raising of people's living standard and the enhancing of health perception, the development of biopesticide and exploitation just progressively receive agricultural chemicals R&D worker's concern, become the focus of agriculture and forestry sustainable development, simultaneously, also be to improve environment, the effective way of minimizing or delaying drug resistance and raising drug effect.Wherein, botanical pesticide has become one of focus of biopesticide research.
Kitchen garden is Portulacaceae annual herb plant.Kitchen garden contains the multiple nutritional components such as protein, fat, carbohydrate, dietary fiber, calcium, phosphorus, iron, copper, carotin, vitamin B1, vitamin B2, niacin, vitamin C, especially the mineral matter such as the vitamin such as vitamin A, vitamin C, vitamin b3 and calcium, iron.Kitchen garden complete stool be used as medicine there is removing toxic substances, the drug effect such as that inhibiting bacteria and diminishing inflammation, diuresis stop dysentery, ease constipation disappears is stagnant, debug, improving eyesight and inhibition uterine hemorrhage; External application can be controlled the diseases such as erysipelas, venomous snake bite.
The rhizome of Chinese monkshood, is the plant of Ranunculaceae Aconitum, contains multiple alkaloid in the rhizome of Chinese monkshood, as Hypaconitine, mesaconine, aconitine, chuan-wu base A, chuan-wu base B (Ka meter Cha Lin), talaisamine etc.In test, the present inventor finds that kitchen garden and the common extract of the rhizome of Chinese monkshood and purslane extract and aconite extract mix and have good synergistic function, can be prepared into insecticide, cabbage caterpillar, diamond-back moth, prodenia litura, beet snout moth's larva etc. are had to good preventive effect.
Summary of the invention
The technical problem to be solved in the present invention is to provide containing the composition pesticide of kitchen garden and aconite extract and preparation method and application.
The present invention is containing the composition pesticide of kitchen garden and aconite extract, and its active component is formed by the crude drug extraction of following weight portion: 1 part of kitchen garden, 0.2～4 part of the rhizome of Chinese monkshood.Wherein, active component is preferably formed by the crude drug extraction of following weight portion: 1 part of kitchen garden, 0.5～2 part of the rhizome of Chinese monkshood; The crude drug extraction being preferably again by following weight portion forms: 1 part of kitchen garden, 0.5～1.5 part of the rhizome of Chinese monkshood; The more preferably crude drug extraction by following weight portion forms: 1 part of kitchen garden, 1 part of the rhizome of Chinese monkshood.
Wherein, kitchen garden and the rhizome of Chinese monkshood that the present invention adopts are Chinese herbal medicine, and in order to improve the present invention containing the activity of the composition pesticide of kitchen garden and aconite extract, kitchen garden is dry kitchen garden herb preferably, and the rhizome of Chinese monkshood is dry aconite root preferably.
The present invention is containing the composition pesticide of kitchen garden and aconite extract, and after its active component can be mixed by crude drug kitchen garden and the rhizome of Chinese monkshood, common extraction forms; Also can first kitchen garden be extracted and obtain purslane extract separately, the rhizome of Chinese monkshood extracts and obtains aconite extract separately, then two kinds of extracts are mixed.In order to improve the insecticidal properties of composition pesticide of the present invention, after its active component is preferably and is mixed by kitchen garden and the rhizome of Chinese monkshood, common extraction forms.
Further, in order to improve the stability containing the composition pesticide of kitchen garden and aconite extract, in the composition pesticide of the present invention containing kitchen garden and aconite extract, also comprise auxiliary agent, described auxiliary agent can adopt the usual auxiliaries in agricultural chemicals, as at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
Wherein, emulsifier can adopt at least one in non-ionic surface active agent and anion surfactant, described non-ionic surface active agent can be dibenzyl xenyl APEO, styryl phenyl APEO, styryl phenyl APEO, phenethyl phenyl polyoxyethylene one polyethenoxy ether, OPEO, alkylphenol polyoxyethylene sulfosuccinate, aliphatic acid polyethenoxy ether, fatty alcohol-polyoxyethylene ether, aliphatic amine polyoxyethylene ether, castor oil ethylene oxide adduct and derivative thereof, at least one in polyol fatty acid ester and ethylene oxide adduct etc. thereof, described anion surfactant is at least one in calcium dodecyl benzene sulfonate, alkylphenol polyoxyethylene phosphate, phenethyl phenol polyethenoxy ether phosphate etc., as emulsifier 500,601p, 602p etc., in addition, can also use commercially available emulsifier production.
Wherein, solvent can adopt at least one in water, ethanol, isopropyl alcohol, n-butanol, ethyl acetate, butyl acetate, methyl-sulfoxide, turpentine oil, solvent naphtha, 1-METHYLPYRROLIDONE, dimethyl formamide etc.
Wherein, antifreeze can be glycerine, propane diols, ethylene glycol, sodium chloride, calcium chloride, sorbierite etc.
Wherein, defoamer is silicone defoaming agent etc.
Wherein, dispersant is at least one in lignosulfonates, PAA and propionamide copolymer, alkylnaphthalene sulfonate, polycarboxylic acid salt's condensation product, methyl sodiosul foaliphatate etc.
Wherein, wetting agent can be alkyl sulfate, alkylbenzenesulfonate, alkylnaphthalene sulfonate, aliphatic alcohol polyethenoxy base ether, alkylphenol-polyethenoxy base ether, sorbitan fatty acid ester etc.
Wherein, stabilizing agent can be 2-expoxy propane, butyl glycidyl ether, isopropyl alcohol, n-butanol, polyethylene glycol etc.
The present invention can be prepared into by the method for this area routine containing the composition pesticide of kitchen garden and aconite extract any one formulation that is applicable to agricultural use, for the ease of storing and transportation, can make microemulsion, aqua or soluble concentrate.
Further, be preferably the aqua that the component by following weight portion forms: kitchen garden and aconite extract, 10 parts of ethanol, 1601,5 parts of ethylene glycol of 8 parts of emulsifier, 57 parts of water, wherein, kitchen garden and aconite extract are that the crude drug of 20 parts extracts and forms.
For the ease of storing and transportation, can extract is concentrated, obtain concentrate, and then be prepared into various formulations, cycles of concentration can be adjusted voluntarily according to needs of production, as preferred version, kitchen garden is prepared from by the following method with aconite extract: crude drug is identical with crude drug weight through extracting and being concentrated into.
Second technical problem that the present invention will solve is to provide preparation containing the method for the composition pesticide of kitchen garden and aconite extract.
The present invention is comprised of following steps containing the preparation method of the composition pesticide of kitchen garden and aconite extract:
A, extraction: take kitchen garden particle and rhizome of Chinese monkshood particle, mix, by solid-liquid ratio, 1:3～12g/ml adds solvent, in 60～95 ℃ of refluxing extraction, filter, and obtains extract, and wherein, described solvent is organic solvent or water;
B, preparation: extract is made to microemulsion, soluble concentrate or aqua.
Wherein, kitchen garden particle and rhizome of Chinese monkshood particle can adopt commercially available particle, also can adopt conventional method pulverizing kitchen garden herb and aconite root to obtain.In order to improve the effect of extraction, simultaneously cost-saving, kitchen garden particle and rhizome of Chinese monkshood particle are crossed 60～100 mesh sieves, preferred mistake 80 mesh sieves.
Further, for cost-saving, simultaneously also in order to improve extraction efficiency, the solid-liquid ratio in a step is preferably 1:4～10g/ml, more preferably 1:6g/ml.The kitchen garden and the rhizome of Chinese monkshood particle that are lg can add 3～12ml solvent extraction, preferably add 4～10ml, more preferably add 6ml.
Conventional organic solvent all can be used for the present invention, for cost-saving, improves dissolution rate, guarantees extraction efficiency simultaneously, and organic solvent is preferably methyl alcohol, ethanol, ethyl acetate, acetone, chloroform, the ethanol that more preferably volume fraction is 95%.
In order to shorten preparation time, accelerate extraction rate, can when adding hot reflux, stir, mixing speed is preferably 80～100 revs/min.
Further, reflux temperature is preferably 75～85 ℃, more preferably 82 ℃; Reflow's cycle is twice, and each return time is 2.5 hours, and merging filtrate, obtains extract.
The present invention also provides the application in control crop pest of composition pesticide that the present invention contains kitchen garden and aconite extract.The present invention is containing the composition pesticide of kitchen garden and aconite extract, and lepidoptera pest is had to good preventive effect as cabbage caterpillar, diamond-back moth, prodenia litura, beet snout moth's larva, tea tree tea geometrid etc.
In actual agricultural production, in order to improve composition pesticide containing kitchen garden and the aconite extract control efficiency to insect pest, the described composition pesticide containing kitchen garden and aconite extract is preferably applied to crop pest initial stage of origination; The present invention can use by common method containing the composition pesticide of kitchen garden and aconite extract, sprays on crop blade face.As preferred version, while using, the present invention is diluted with water to containing the composition pesticide of kitchen garden and aconite extract to take the activity component concentration of crude drug weighing scale be 10～100ppm, it is the composition pesticide making after 1g crude drug extracts, when using, be diluted with water to 10kg, its activity component concentration equals 100ppm.Further preferably being diluted to activity component concentration is that 20～50ppm sprays in plant blade face, and to occur water membrane on blade face, not lower of liquid is advisable; After being preferable over 3～5 days, carry out secondary dispenser, the preferred continuous administration of a season crop 2～3 times.
The present invention is mixed by purslane extract and aconite extract containing the composition pesticide of kitchen garden and aconite extract, or formed by kitchen garden and common extraction of the rhizome of Chinese monkshood, its preventive effect is far away higher than the extract of single component, particularly kitchen garden and the rhizome of Chinese monkshood are mixed to rear common extraction, owing to having solubilising, hydrotropy effect between kitchen garden and the rhizome of Chinese monkshood, the composition pesticide insecticidal effect of making after common extraction is more remarkable.
Compared with prior art, the present invention has the following advantages containing the composition pesticide of kitchen garden and aconite extract:
1, the present invention has synergistic function containing the composition pesticide of kitchen garden and aconite extract, obvious to the control efficiency of lepidoptera pest.
2, kitchen garden of the present invention and the rhizome of Chinese monkshood are common extracts or extracts respectively rear mixing, and the composition pesticide of making can improve crop immune system ability in quick-acting tagging, and can significantly improve its vigor.
3, the present invention be take Chinese herbal medicine as raw material, thereby does not have Pesticide Residue, and insect is difficult to produce resistance; Compare with independent use chemical source agricultural chemicals, environmentally friendly.
When 4, the present invention uses containing the composition pesticide of kitchen garden and aconite extract, valid density is low, with low cost.
Embodiment
The present invention is containing the composition pesticide of kitchen garden and aconite extract, and its active component is formed by the crude drug extraction of following weight portion: 1 part of kitchen garden, 0.2～4 part of the rhizome of Chinese monkshood.Wherein, active component is preferably formed by the crude drug extraction of following weight portion: 1 part of kitchen garden, 0.5～2 part of the rhizome of Chinese monkshood; The crude drug extraction being preferably again by following weight portion forms: 1 part of kitchen garden, 0.5～1.5 part of the rhizome of Chinese monkshood; The more preferably crude drug extraction by following weight portion forms: 1 part of kitchen garden, 1 part of the rhizome of Chinese monkshood.
Wherein, kitchen garden and the rhizome of Chinese monkshood that the present invention adopts are Chinese herbal medicine, and in order to improve the present invention containing the activity of the composition pesticide of kitchen garden and aconite extract, kitchen garden is dry kitchen garden herb preferably, and the rhizome of Chinese monkshood is dry aconite root preferably.
The present invention is containing the composition pesticide of kitchen garden and aconite extract, and after its active component can be mixed by crude drug kitchen garden and the rhizome of Chinese monkshood, common extraction forms; Also can first kitchen garden be extracted and obtain purslane extract separately, the rhizome of Chinese monkshood extracts and obtains aconite extract separately, then two kinds of extracts are mixed.In order to improve the insecticidal properties of composition pesticide of the present invention, after its active component is preferably and is mixed by kitchen garden and the rhizome of Chinese monkshood, common extraction forms.
Further, in order to improve the stability containing the composition pesticide of kitchen garden and aconite extract, in the composition pesticide of the present invention containing kitchen garden and aconite extract, also comprise auxiliary agent, described auxiliary agent can adopt the usual auxiliaries in agricultural chemicals, as at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
Wherein, emulsifier can adopt at least one in non-ionic surface active agent and anion surfactant, described non-ionic surface active agent can be dibenzyl xenyl APEO, styryl phenyl APEO, styryl phenyl APEO, phenethyl phenyl polyoxyethylene one polyethenoxy ether, OPEO, alkylphenol polyoxyethylene sulfosuccinate, aliphatic acid polyethenoxy ether, fatty alcohol-polyoxyethylene ether, aliphatic amine polyoxyethylene ether, castor oil ethylene oxide adduct and derivative thereof, at least one in polyol fatty acid ester and ethylene oxide adduct etc. thereof, described anion surfactant is at least one in calcium dodecyl benzene sulfonate, alkylphenol polyoxyethylene phosphate, phenethyl phenol polyethenoxy ether phosphate etc., as emulsifier 500,601p, 602p etc., in addition, can also use commercially available emulsifier production.
Wherein, solvent can adopt at least one in water, ethanol, isopropyl alcohol, n-butanol, ethyl acetate, butyl acetate, methyl-sulfoxide, turpentine oil, solvent naphtha, 1-METHYLPYRROLIDONE, dimethyl formamide etc.
Wherein, antifreeze can be glycerine, propane diols, ethylene glycol, sodium chloride, calcium chloride, sorbierite etc.
Wherein, defoamer is silicone defoaming agent etc.
Wherein, dispersant is at least one in lignosulfonates, PAA and propionamide copolymer, alkylnaphthalene sulfonate, polycarboxylic acid salt's condensation product, methyl sodiosul foaliphatate etc.
Wherein, wetting agent can be alkyl sulfate, alkylbenzenesulfonate, alkylnaphthalene sulfonate, aliphatic alcohol polyethenoxy base ether, alkylphenol-polyethenoxy base ether, sorbitan fatty acid ester etc.
Wherein, stabilizing agent can be 2-expoxy propane, butyl glycidyl ether, isopropyl alcohol, n-butanol, polyethylene glycol etc.
The present invention can be prepared into by the method for this area routine containing the composition pesticide of kitchen garden and aconite extract any one formulation that is applicable to agricultural use, for the ease of storing and transportation, can make microemulsion, aqua or soluble concentrate.
Further, be preferably the aqua that the component by following weight portion forms: kitchen garden and aconite extract, 10 parts of ethanol, 1601,5 parts of ethylene glycol of 8 parts of emulsifier, 57 parts of water, wherein, kitchen garden and aconite extract are that the crude drug of 20 parts extracts and forms.
For the ease of storing and transportation, can extract is concentrated, obtain concentrate, and then be prepared into various formulations, cycles of concentration can be adjusted voluntarily according to needs of production, as preferred version, kitchen garden is prepared from by the following method with aconite extract: crude drug is identical with crude drug weight through extracting and being concentrated into.
The present invention is comprised of following steps containing the preparation method of the composition pesticide of kitchen garden and aconite extract:
A, extraction: take kitchen garden particle and rhizome of Chinese monkshood particle, mix, by solid-liquid ratio, 1:3～12g/ml adds solvent, in 60～95 ℃ of refluxing extraction, filter, and obtains extract, and wherein, described solvent is organic solvent or water;
B, preparation: extract is made to microemulsion, soluble concentrate or aqua.
Wherein, kitchen garden particle and rhizome of Chinese monkshood particle can adopt commercially available particle, also can adopt conventional method pulverizing kitchen garden herb and aconite root to obtain.In order to improve the effect of extraction, simultaneously cost-saving, kitchen garden particle and rhizome of Chinese monkshood particle are crossed 60～100 mesh sieves, preferred mistake 80 mesh sieves.
Further, for cost-saving, simultaneously also in order to improve extraction efficiency, the solid-liquid ratio in a step is preferably 1:4～10g/ml, more preferably 1:6g/ml.The kitchen garden and the rhizome of Chinese monkshood particle that are lg can add 3～12ml solvent extraction, preferably add 4～10ml, more preferably add 6ml.
Conventional organic solvent all can be used for the present invention, for cost-saving, improves dissolution rate, guarantees extraction efficiency simultaneously, and organic solvent is preferably methyl alcohol, ethanol, ethyl acetate, acetone, chloroform, the ethanol that more preferably volume fraction is 95%.
In order to shorten preparation time, accelerate extraction rate, can when adding hot reflux, stir, mixing speed is preferably 80～100 revs/min.
Further, reflux temperature is preferably 75～85 ℃, more preferably 82 ℃; Reflow's cycle is twice, and each return time is 2.5 hours, and merging filtrate, obtains extract.
The present invention is containing the composition pesticide of kitchen garden and aconite extract, and lepidoptera pest is had to good preventive effect as cabbage caterpillar, diamond-back moth, prodenia litura, beet snout moth's larva, tea tree tea geometrid etc.
In actual agricultural production, in order to improve composition pesticide containing kitchen garden and the aconite extract control efficiency to insect pest, the described composition pesticide containing kitchen garden and aconite extract is preferably applied to crop pest initial stage of origination; The present invention can use by common method containing the composition pesticide of kitchen garden and aconite extract, sprays on crop blade face.As preferred version, while using, the present invention is diluted with water to containing the composition pesticide of kitchen garden and aconite extract to take the activity component concentration of crude drug weighing scale be 10～100ppm, it is the composition pesticide making after 1g crude drug extracts, when using, be diluted with water to 10kg, its activity component concentration equals 100ppm.Further preferably being diluted to activity component concentration is that 20～50ppm sprays in plant blade face, and to occur water membrane on blade face, not lower of liquid is advisable; After being preferable over 3～5 days, carry out secondary dispenser, the preferred continuous administration of a season crop 2～3 times.
The present invention is mixed by purslane extract and aconite extract containing the composition pesticide of kitchen garden and aconite extract, or formed by kitchen garden and common extraction of the rhizome of Chinese monkshood, its preventive effect is far away higher than the extract of single component, particularly kitchen garden and the rhizome of Chinese monkshood are mixed to rear common extraction, owing to having solubilising, hydrotropy effect between kitchen garden and the rhizome of Chinese monkshood, the composition pesticide insecticidal effect of making after common extraction is more remarkable.
Below in conjunction with embodiment, the specific embodiment of the present invention is further described, does not therefore limit the present invention among described scope of embodiments.
Embodiment 1 is containing the preparation of the composition pesticide of kitchen garden and aconite extract
A, extraction: take dry kitchen garden herb and aconite root, mix, pulverize, cross 80 mesh sieves, add extraction solution, refluxing extraction twice, filters, and merging filtrate is concentrated, makes extract weight equal the weight of crude drug, obtains extract mw-01～mw-06; Wherein, each parameter is in Table 1;
B, preparation: auxiliary agent add table 2 in extract mw-01～mw-06 in, stir, must be containing the composition pesticide MW-01～MW-06 of kitchen garden and aconite extract.
The preparation technology parameter of table 1 kitchen garden and aconite extract
Wherein, kitchen garden herb and aconite root are all purchased from Anguo City Zhong Tian prepared slices of Chinese crude drugs Co., Ltd.
Table 2 is containing the formula of the composition pesticide of kitchen garden and aconite extract
Embodiment 2 is containing the preparation of the composition pesticide of kitchen garden and aconite extract
A, extraction: take dry kitchen garden herb, pulverize, cross 80 mesh sieves, add extraction solution, refluxing extraction twice, filters, and merging filtrate is concentrated, makes extract weight equal the weight of crude drug, obtains extract d1-01～d1-03, and wherein, each parameter is in Table 3;
Take dry aconite root, pulverize, cross 80 mesh sieves, add extraction solution, refluxing extraction twice, filters, and merging filtrate is concentrated, makes extract weight equal the weight of crude drug, obtains extract d2-01～d2-03, and wherein, each parameter is in Table 4;
B, preparation: take kitchen garden extract, rhizome of Chinese monkshood extract and auxiliary agent, stirring and evenly mixing, obtains the composition pesticide MW-07～MW-12 containing kitchen garden and aconite extract, and wherein, each parameter is in Table 5.
Table 3
Table 4
Table 5
Test example 1 the present invention is containing the efficiency test of the composition pesticide wild cabbage diamond-back moth of kitchen garden and aconite extract
For clearly putting into field efficacy situation containing the composition pesticide of kitchen garden and aconite extract, inventor has carried out field control effectiveness test to this medicament.
1, test material
1.1 for studying thing and object: wild cabbage diamond-back moth
1.2 reagent agent
Product in embodiment: MW-01～MW-12;
Kitchen garden single dose (D1), rhizome of Chinese monkshood single dose (D2):
Chemical reagent contrast (CK-01): gamma cyhalothrin, purchased from the biochemical Co., Ltd of Chengdu Ke Lilong;
Clear water contrast (CK-02).
Wherein, the preparation method of kitchen garden single dose is: get the d1-03 of 20g, add 10g ethanol, and 8g emulsifier 1601,5g ethylene glycol, 57g water, stirring and evenly mixing, makes kitchen garden single dose.
The preparation method of rhizome of Chinese monkshood single dose is: gets the d2-03 of 20g, adds 10g ethanol, and 8g emulsifier 1601,5g ethylene glycol, 57g water, stirring and evenly mixing, makes rhizome of Chinese monkshood single dose.
2, experimental scheme and method
14 medicaments of this test, 1 contrast medicament, 1 blank, repeats Gong48Ge community for three times.Minizone and surrounding all leave isolation strip, community area 12m
2, randomized arrangement is respectively processed in community.
By reagent agent thin up, to activity component concentration, be 50ppm, spray application to plant blade face, to occur water membrane on blade face, not lower of liquid is advisable.
Before test, carry out diamond-back moth quantity statistics alive the listing mark of fixed blade.Within 5 days after dispenser, investigate and record the diamond-back moth number alive on each blade.Calculate insect population decline rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
3, result of the test
Within 5 days after dispenser, investigate and record the borer population alive on each blade, calculate insect population decline rate and preventive effect, it the results are shown in Table 6.
Table 6
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Insect population decline rate (%) | Preventive effect (%) |
| MW-01 | 337 | 72 | 78.64 | 81.85 |
| MW-02 | 347 | 47 | 86.46 | 88.50 |
| MW-03 | 352 | 39 | 88.92 | 90.59 |
| MW-04 | 318 | 41 | 87.11 | 89.05 |
| MW-05 | 386 | 62 | 83.94 | 86.36 |
| MW-06 | 394 | 70 | 82.23 | 84.91 |
| MW-07 | 331 | 96 | 71.00 | 75.37 |
| MW-08 | 358 | 87 | 75.70 | 79.36 |
| MW-09 | 369 | 82 | 77.78 | 81.13 |
Continued 6
| MW-10 | 306 | 71 | 76.80 | 80.29 |
| MW-11 | 370 | 97 | 73.78 | 77.73 |
| MW-12 | 388 | 108 | 72.16 | 76.36 |
| D1 | 316 | 278 | 12.03 | 25.28 |
| D2 | 329 | 241 | 26.75 | 37.78 |
| CK-01 | 343 | 32 | 90.67 | 92.08 |
| CK-02 | 327 | 385 | -17.74 | —— |
As can be seen from Table 6, product of the present invention has good preventive effect to diamond-back moth, and better than the control efficiency of single purslane extract and aconite extract, visible, between product compositions of the present invention, has synergistic function.And the preventive effect that adopts the product that common extracting method makes is apparently higher than blended product after independent extraction, visible common extraction contributes to improve dissolution rate, improves insecticide efficiency.
Test example 2 the present invention are containing the efficiency test of the composition pesticide tea tree tea geometrid of kitchen garden and aconite extract
Product MW-01～MW-12 of the present invention is used for preventing and treating tea tree tea geometrid.12 medicaments of this test, 2 single doses, 1 blank, repeats Gong45Ge community for three times.Minizone and surrounding all leave isolation strip, community area 10m
2, randomized arrangement is respectively processed in community.
By reagent agent thin up, to activity component concentration, be 50ppm, spray application to plant blade face, to occur water membrane on blade face, not lower of liquid is advisable.
Before test, carry out tea geometrid quantity statistics alive the listing mark of fixed blade.Within 5 days after dispenser, investigate and record the tea geometrid number alive on each blade.Calculate insect population decline rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group meter that repeats to average, calculates insect population decline rate and preventive effect, and it the results are shown in Table 7.
Table 7
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Insect population decline rate (%) | Preventive effect (%) |
| MW-01 | 212 | 54 | 74.53 | 77.60 |
Continued 7
| MW-02 | 245 | 47 | 80.82 | 83.13 |
| MW-03 | 256 | 43 | 83.20 | 85.23 |
| MW-04 | 231 | 42 | 81.82 | 84.01 |
| MW-05 | 255 | 52 | 79.61 | 82.06 |
| MW-06 | 227 | 50 | 77.97 | 80.63 |
| MW-07 | 285 | 103 | 63.86 | 68.21 |
| MW-08 | 294 | 86 | 70.75 | 74.27 |
| MW-09 | 246 | 65 | 73.58 | 76.76 |
| MW-10 | 215 | 61 | 71.63 | 75.04 |
| MW-11 | 256 | 79 | 69.14 | 72.86 |
| MW-12 | 268 | 89 | 66.79 | 70.79 |
| D1 | 235 | 203 | 13.62 | 24.02 |
| D2 | 233 | 182 | 21.89 | 31.29 |
| CK-02 | 263 | 299 | -13.69 | —— |
As can be seen from Table 7, product of the present invention has good preventive effect to tea geometrid, and better than the control efficiency of single purslane extract and aconite extract, visible, between product compositions of the present invention, has synergistic function.And the preventive effect that adopts the product that common extracting method makes is apparently higher than blended product after independent extraction, visible common extraction contributes to improve dissolution rate, improves insecticide efficiency.In addition, during dispenser, have no crop is had to poisoning performance.
Test example 3 the present invention are containing the efficiency test of the composition pesticide wild cabbage prodenia litura of kitchen garden and aconite extract
Product MW-01～MW-12 of the present invention is used for preventing and treating wild cabbage prodenia litura.12 medicaments of this test, 2 single doses, 1 blank, repeats Gong45Ge community for three times.Minizone and surrounding all leave isolation strip, community area 20m
2, randomized arrangement is respectively processed in community.
By reagent agent thin up, to activity component concentration, be 50ppm, spray application to plant blade face, to occur water membrane on blade face, not lower of liquid is advisable.
Before test, carry out prodenia litura quantity statistics alive the listing mark of fixed blade.Within 5 days after dispenser, investigate and record the prodenia litura number alive on each blade.Calculate insect population decline rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group meter that repeats to average, calculates insect population decline rate and preventive effect, and its result is as shown in table 8.
Table 8
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Insect population decline rate (%) | Preventive effect (%) |
| MW-01 | 321 | 87 | 72.90 | 76.36 |
| MW-02 | 325 | 67 | 79.38 | 82.02 |
| MW-03 | 346 | 63 | 81.79 | 84.12 |
| MW-04 | 334 | 68 | 79.64 | 82.24 |
| MW-05 | 311 | 67 | 78.46 | 81.21 |
| MW-06 | 367 | 86 | 76.57 | 79.56 |
| MW-07 | 385 | 142 | 63.12 | 67.83 |
| MW-08 | 342 | 102 | 70.18 | 73.98 |
| MW-09 | 398 | 111 | 72.11 | 75.67 |
| MW-10 | 377 | 112 | 70.29 | 74.08 |
| MW-11 | 365 | 118 | 67.67 | 71.80 |
| MW-12 | 383 | 128 | 66.58 | 70.85 |
| D1 | 392 | 335 | 14.54 | 25.45 |
| D2 | 357 | 283 | 20.73 | 30.85 |
| CK-02 | 328 | 376 | -14.63 | —— |
As can be seen from Table 8, product of the present invention has good preventive effect to prodenia litura, and better than the control efficiency of single purslane extract and aconite extract, visible, between product compositions of the present invention, has synergistic function.And the preventive effect that adopts the product that common extracting method makes is apparently higher than blended product after independent extraction, visible common extraction contributes to improve dissolution rate, improves insecticide efficiency.In addition, during dispenser, have no crop is had to poisoning performance.
Test example 4 the present invention are containing the efficiency test of the composition pesticide cabbage pieris of kitchen garden and aconite extract
Product MW-01～MW-12 of the present invention is used for preventing and treating cabbage pieris.12 medicaments of this test, 2 single doses, 1 blank, repeats Gong45Ge community for three times.Minizone and surrounding all leave isolation strip, community area 10m
2, randomized arrangement is respectively processed in community.
By reagent agent thin up, to activity component concentration, be 50ppm, spray application to plant blade face, to occur water membrane on blade face, not lower of liquid is advisable.
Before test, carry out cabbage caterpillar quantity statistics alive the listing mark of fixed blade.Within 5 days after dispenser, investigate and record the dark greyish green borer population alive on each blade.Calculate insect population decline rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group meter that repeats to average, calculates insect population decline rate and preventive effect, and its result is as shown in table 9.
Table 9
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Insect population decline rate (%) | Preventive effect (%) |
| MW-01 | 378 | 75 | 80.16 | 83.74 |
| MW-02 | 324 | 56 | 82.72 | 85.84 |
| MW-03 | 356 | 59 | 83.43 | 86.42 |
| MW-04 | 347 | 59 | 83.00 | 86.07 |
| MW-05 | 351 | 64 | 81.77 | 85.06 |
| MW-06 | 397 | 74 | 81.36 | 84.73 |
| MW-07 | 388 | 123 | 68.30 | 74.02 |
| MW-08 | 358 | 102 | 71.51 | 76.65 |
| MW-09 | 342 | 94 | 72.51 | 77.48 |
| MW-10 | 331 | 93 | 71.90 | 76.98 |
| MW-11 | 379 | 112 | 70.45 | 75.78 |
| MW-12 | 337 | 102 | 69.73 | 75.20 |
| D1 | 326 | 289 | 11.35 | 27.35 |
| D2 | 377 | 301 | 20.16 | 34.57 |
| CK-02 | 345 | 421 | -22.03 | —— |
As can be seen from Table 9, product of the present invention has good preventive effect to cabbage caterpillar, and better than the control efficiency of single purslane extract and aconite extract, visible, between product compositions of the present invention, has synergistic function.And the preventive effect that adopts the product that common extracting method makes is apparently higher than blended product after independent extraction, visible common extraction contributes to improve dissolution rate, improves insecticide efficiency.In addition, during dispenser, have no crop is had to poisoning performance.
Claims (10)
1. containing the composition pesticide of kitchen garden and aconite extract, it is characterized in that, its active component is formed by the crude drug extraction of following weight portion: 1 part of kitchen garden, 0.2～4 part of the rhizome of Chinese monkshood.
2. the composition pesticide containing kitchen garden and aconite extract according to claim 1, is characterized in that, its active component is formed by the crude drug extraction of following weight portion: 1 part of kitchen garden, 0.5～2 part of the rhizome of Chinese monkshood; Be preferably 1 part of kitchen garden, 0.5～1.5 part of the rhizome of Chinese monkshood; More preferably kitchen garden is 1 part, 1 part of the rhizome of Chinese monkshood.
3. the composition pesticide containing kitchen garden and aconite extract according to claim 1 and 2, is characterized in that: after its active component is mixed by kitchen garden and the rhizome of Chinese monkshood, common extraction forms; Or by kitchen garden, extract the purslane extract, the rhizome of Chinese monkshood that obtain and extract the aconite extract obtaining and mix.
4. according to the composition pesticide containing kitchen garden and aconite extract described in claim 1～3 any one, it is characterized in that: the described composition pesticide containing kitchen garden and aconite extract is microemulsion, aqua or soluble concentrate.
5. according to the composition pesticide containing kitchen garden and aconite extract described in claim 1～4 any one, it is characterized in that: in the described composition pesticide containing kitchen garden and aconite extract, also comprise auxiliary agent, described auxiliary agent is at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
6. the composition pesticide containing kitchen garden and aconite extract according to claim 5, it is characterized in that: described aqua is comprised of the component of following weight portion: kitchen garden and aconite extract, 10 parts of ethanol, 8 parts of emulsifier 1601,5 parts of ethylene glycol, 57 parts of water, wherein, kitchen garden and aconite extract are that the crude drug extraction of 20 parts forms; Kitchen garden is preferably prepared from by the following method with aconite extract: crude drug is identical with crude drug weight through extracting and being concentrated into.
7. the method for the composition pesticide containing kitchen garden and aconite extract described in preparation claim 1～6 any one, is characterized in that being comprised of following steps:
A, extraction: take kitchen garden particle and rhizome of Chinese monkshood particle, mix, by solid-liquid ratio, 1:3～12g/ml adds solvent, in 60～95 ℃ of refluxing extraction, filter, and obtains extract, and wherein, described solvent is organic solvent or water;
B, preparation: extract is made to microemulsion, soluble concentrate or aqua.
8. preparation according to claim 7, containing the method for the composition pesticide of kitchen garden and aconite extract, is characterized in that: in described a step, kitchen garden particle and rhizome of Chinese monkshood particle are crossed 60～100 mesh sieves preferred mistake 80 mesh sieves; Solid-liquid ratio is 1:4～10g/ml, is preferably 1:6g/ml; Organic solvent is methyl alcohol, ethanol, ethyl acetate, acetone, chloroform, is preferably volume fraction and is 95% ethanol; Reflux temperature is 75～85 ℃, is preferably 82 ℃; Reflow's cycle is twice, and merging filtrate, obtains extract.
9. the application of the composition pesticide containing kitchen garden and aconite extract described in claim 1～6 any one in control crop pest.
10. the application of the composition pesticide containing kitchen garden and aconite extract according to claim 9 in control crop pest, is characterized in that: described insect pest is lepidoptera pest; During application, be diluted with water to that to take the activity component concentration of crude drug weighing scale be 10～100ppm, be preferably diluted with water to that to take the activity component concentration of crude drug weighing scale be 20～50ppm.
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| CN104472558A (en) * | 2014-11-17 | 2015-04-01 | 柳州市天姿园艺有限公司 | Kapok seed treatment agent for control of chafer insect attack |
| CN106135311A (en) * | 2015-04-08 | 2016-11-23 | 黄武 | One vegetative insecticide |
| CN108112638A (en) * | 2016-11-30 | 2018-06-05 | 华南农业大学 | Purslane extract is preparing the application in killing prodenia litura drug |
| CN108849900A (en) * | 2018-07-04 | 2018-11-23 | 华南农业大学 | A kind of efficient insect resistant agent and preparation method thereof and its application |
| CN111685131A (en) * | 2020-06-15 | 2020-09-22 | 山东省农业科学院植物保护研究所 | Pharmaceutical composition for preventing peanut rust by using purslane extract and preparation method thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104472558A (en) * | 2014-11-17 | 2015-04-01 | 柳州市天姿园艺有限公司 | Kapok seed treatment agent for control of chafer insect attack |
| CN106135311A (en) * | 2015-04-08 | 2016-11-23 | 黄武 | One vegetative insecticide |
| CN108112638A (en) * | 2016-11-30 | 2018-06-05 | 华南农业大学 | Purslane extract is preparing the application in killing prodenia litura drug |
| CN108849900A (en) * | 2018-07-04 | 2018-11-23 | 华南农业大学 | A kind of efficient insect resistant agent and preparation method thereof and its application |
| CN111685131A (en) * | 2020-06-15 | 2020-09-22 | 山东省农业科学院植物保护研究所 | Pharmaceutical composition for preventing peanut rust by using purslane extract and preparation method thereof |
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