CN105238754B - 一种高增殖力和高杀伤力nk细胞的体外培养方法 - Google Patents
一种高增殖力和高杀伤力nk细胞的体外培养方法 Download PDFInfo
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Abstract
Description
培养天数(天) | 0 | 7 | 14 | 21 |
实施例4 | 1 | 12.55±3.26 | 41.72±4.12 | 82.57±4.78 |
实施例1 | 1 | 29.65±3.75** | 79.82±5.17** | 159.95±5.14** |
Claims (10)
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CN105238754B true CN105238754B (zh) | 2016-08-17 |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN106834227B (zh) * | 2016-12-26 | 2020-01-24 | 深圳精准医疗科技有限公司 | 自然杀伤细胞的体外纯化方法 |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
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CN105907714A (zh) * | 2016-04-28 | 2016-08-31 | 王晓冰 | 一种改良的nk细胞培养方法 |
CN108220235A (zh) * | 2016-12-22 | 2018-06-29 | 细胞邦(北京)生物技术有限公司 | 一种体外活化扩增人自然杀伤(nk)细胞的方法及其专用培养体系 |
CN106591233B (zh) * | 2016-12-28 | 2018-01-09 | 广州沙艾生物科技有限公司 | 一种免疫细胞的体外诱导扩增和冻存的方法 |
CN106701681B (zh) * | 2016-12-28 | 2018-01-09 | 广州沙艾生物科技有限公司 | 一种免疫细胞的体外诱导扩增、冻存和复苏的方法 |
CN107022524B (zh) * | 2017-03-14 | 2019-10-22 | 上海莱馥医疗科技有限公司 | 一种从外周血单个核细胞中大量扩增nk细胞的方法 |
CN108300694B (zh) * | 2018-02-07 | 2020-10-20 | 安徽古一生物科技有限公司 | 一种nk细胞的无血清培养方法 |
CN109394785A (zh) * | 2018-09-25 | 2019-03-01 | 天津市康婷生物工程有限公司 | 一种nk细胞预防黑色素瘤发生的实验方法 |
CN109207424A (zh) * | 2018-10-24 | 2019-01-15 | 河南省肿瘤医院 | 一种免疫细胞的培养方法 |
CN111394309A (zh) * | 2019-12-10 | 2020-07-10 | 广东先康达生物科技有限公司 | 一种nk细胞体外扩增培养的方法 |
CN112300992B (zh) * | 2020-10-27 | 2023-05-23 | 杜德(江门)生物科技有限公司 | 一种nk细胞培养液及多级激活nk细胞的培养方法 |
CN113388042B (zh) * | 2021-06-28 | 2022-02-01 | 北京鼎成肽源生物技术有限公司 | 一种重组蛋白、重组表达载体、重组细胞和nk细胞激活磁珠及其制备方法和应用 |
CN114891740B (zh) * | 2022-03-25 | 2023-12-01 | 和携科技有限公司 | 一种高增殖能力和高细胞毒性nk细胞的培养方法及其应用 |
Citations (1)
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CN102994449A (zh) * | 2012-12-13 | 2013-03-27 | 上海柯莱逊生物技术有限公司 | 一种体外扩增nk细胞的方法 |
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CN102994449A (zh) * | 2012-12-13 | 2013-03-27 | 上海柯莱逊生物技术有限公司 | 一种体外扩增nk细胞的方法 |
CN103756963A (zh) * | 2012-12-13 | 2014-04-30 | 上海柯莱逊生物技术有限公司 | 一种体外扩增nk细胞的方法 |
Non-Patent Citations (1)
Title |
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新疆地区健康成人外周血NK细胞体外扩增技术的研究;刘悦明等;《新疆医科大学学报》;20130630;第36卷(第6期);763-767 * |
Cited By (1)
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CN106834227B (zh) * | 2016-12-26 | 2020-01-24 | 深圳精准医疗科技有限公司 | 自然杀伤细胞的体外纯化方法 |
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