CN105143458A - 用于产生定制油的硫酯酶和细胞 - Google Patents

用于产生定制油的硫酯酶和细胞 Download PDF

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Publication number
CN105143458A
CN105143458A CN201480020002.5A CN201480020002A CN105143458A CN 105143458 A CN105143458 A CN 105143458A CN 201480020002 A CN201480020002 A CN 201480020002A CN 105143458 A CN105143458 A CN 105143458A
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CN
China
Prior art keywords
seqidno
sequence
oil
nucleic acid
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201480020002.5A
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English (en)
Chinese (zh)
Inventor
G·N·鲁丹科
J·加索拉里
S·富兰克林
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kirbyn Biotech Corp
Original Assignee
Solazyme Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US13/837,996 external-priority patent/US9290749B2/en
Application filed by Solazyme Inc filed Critical Solazyme Inc
Publication of CN105143458A publication Critical patent/CN105143458A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/02—Thioester hydrolases (3.1.2)
    • C12Y301/02014—Oleoyl-[acyl-carrier-protein] hydrolase (3.1.2.14), i.e. ACP-thioesterase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Fats And Perfumes (AREA)
  • Edible Oils And Fats (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201480020002.5A 2013-03-15 2014-03-13 用于产生定制油的硫酯酶和细胞 Pending CN105143458A (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201361791861P 2013-03-15 2013-03-15
US13/837,996 US9290749B2 (en) 2013-03-15 2013-03-15 Thioesterases and cells for production of tailored oils
US13/837,996 2013-03-15
US61/791,861 2013-03-15
US201361917217P 2013-12-17 2013-12-17
US61/917,217 2013-12-17
PCT/US2014/026644 WO2014151904A1 (fr) 2013-03-15 2014-03-13 Thioestérases et cellules pour la production d'huiles à façon

Publications (1)

Publication Number Publication Date
CN105143458A true CN105143458A (zh) 2015-12-09

Family

ID=51581068

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201480020002.5A Pending CN105143458A (zh) 2013-03-15 2014-03-13 用于产生定制油的硫酯酶和细胞

Country Status (9)

Country Link
EP (1) EP2971024A4 (fr)
JP (1) JP2016518112A (fr)
KR (1) KR20150128770A (fr)
CN (1) CN105143458A (fr)
AU (2) AU2014236763B2 (fr)
BR (1) BR112015023192A8 (fr)
CA (1) CA2904395A1 (fr)
MX (1) MX2015011507A (fr)
WO (1) WO2014151904A1 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9816079B2 (en) 2013-01-29 2017-11-14 Terravia Holdings, Inc. Variant thioesterases and methods of use
US9567615B2 (en) 2013-01-29 2017-02-14 Terravia Holdings, Inc. Variant thioesterases and methods of use
US9783836B2 (en) 2013-03-15 2017-10-10 Terravia Holdings, Inc. Thioesterases and cells for production of tailored oils
US9290749B2 (en) 2013-03-15 2016-03-22 Solazyme, Inc. Thioesterases and cells for production of tailored oils
BR112017001404A2 (pt) * 2014-07-24 2017-11-21 Terravia Holdings Inc tioesterases variantes e métodos de utilização
CN107208103A (zh) * 2014-09-18 2017-09-26 泰拉瑞亚控股公司 酰基‑acp硫酯酶及其突变体
US20180142218A1 (en) 2016-10-05 2018-05-24 Terravia Holdings, Inc. Novel acyltransferases, variant thioesterases, and uses thereof
EP4090735A1 (fr) 2020-01-16 2022-11-23 Corbion Biotech, Inc. Variants de bêta-cétoacyl-acp synthase ii

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992020236A1 (fr) * 1991-05-21 1992-11-26 Calgene, Inc. Thioesterases a chaine moyenne d'origine vegetale
WO1994010288A2 (fr) * 1992-10-30 1994-05-11 Calgene, Inc. Thioesterases a chaine moyenne provenant de plantes
WO1995013390A2 (fr) * 1993-11-10 1995-05-18 Calgene, Inc. Sequences de thioesterases vegetales actives sur les substrats acyl-(proteine vectrice d'acyle)
CN102066569A (zh) * 2008-06-20 2011-05-18 巴斯夫植物科学有限公司 具有增强的产量相关性状的植物和产生所述植物的方法
CN102459569A (zh) * 2009-04-10 2012-05-16 Ls9公司 脂肪酸衍生物的产生
CN102586350A (zh) * 2012-01-09 2012-07-18 北京化工大学 一种c8:0/c10:0/c12:0/c14:0中链脂肪酸及其乙酯的生产方法
US20130034887A1 (en) * 2011-02-02 2013-02-07 Solazyme, Inc. Tailored Oils Produced from Recombinant Oleaginous Microorganisms

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5298421A (en) 1990-04-26 1994-03-29 Calgene, Inc. Plant medium-chain-preferring acyl-ACP thioesterases and related methods
US5344771A (en) 1990-04-26 1994-09-06 Calgene, Inc. Plant thiosterases
US5639790A (en) 1991-05-21 1997-06-17 Calgene, Inc. Plant medium-chain thioesterases
US5654495A (en) 1992-10-30 1997-08-05 Calgene, Inc. Production of myristate in plant cells
US5850022A (en) 1992-10-30 1998-12-15 Calgene, Inc. Production of myristate in plant cells
EP0716708A1 (fr) * 1993-09-03 1996-06-19 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Thioesterases induisant specifiquement des chaines de longueur moyenne
US5807893A (en) 1993-11-18 1998-09-15 Voelker; Toni Alois Plant thioesterases and use for modification of fatty acid composition in plant seed oils
SG182157A1 (en) 2007-06-01 2012-07-30 Solazyme Inc Production of oil in microorganisms
CA3128584A1 (fr) * 2007-07-09 2009-01-15 BASF Agricultural Solutions Seed US LLC Brassica comprenant des alleles mutants de l'acyle gras-acp thioesterase
US7982035B2 (en) 2007-08-27 2011-07-19 Duquesne University Of The Holy Spirit Tricyclic compounds having antimitotic and/or antitumor activity and methods of use thereof
CN102712858B (zh) 2008-11-28 2015-08-12 索拉兹米公司 在重组异养型微生物中制备特制油
CN103124499B (zh) 2010-05-28 2016-09-28 泰拉瑞亚控股公司 包含特制油的食品组合物
US8951762B2 (en) * 2011-07-27 2015-02-10 Iowa State University Research Foundation, Inc. Materials and methods for using an acyl—acyl carrier protein thioesterase and mutants and chimeras thereof in fatty acid synthesis
CA2870364A1 (fr) 2012-04-18 2013-10-24 Solazyme, Inc. Microbes recombinants renfermant des enzymes de passage synthetiques d'acides gras modifies

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992020236A1 (fr) * 1991-05-21 1992-11-26 Calgene, Inc. Thioesterases a chaine moyenne d'origine vegetale
WO1994010288A2 (fr) * 1992-10-30 1994-05-11 Calgene, Inc. Thioesterases a chaine moyenne provenant de plantes
WO1995013390A2 (fr) * 1993-11-10 1995-05-18 Calgene, Inc. Sequences de thioesterases vegetales actives sur les substrats acyl-(proteine vectrice d'acyle)
CN102066569A (zh) * 2008-06-20 2011-05-18 巴斯夫植物科学有限公司 具有增强的产量相关性状的植物和产生所述植物的方法
CN102459569A (zh) * 2009-04-10 2012-05-16 Ls9公司 脂肪酸衍生物的产生
US20130034887A1 (en) * 2011-02-02 2013-02-07 Solazyme, Inc. Tailored Oils Produced from Recombinant Oleaginous Microorganisms
CN102586350A (zh) * 2012-01-09 2012-07-18 北京化工大学 一种c8:0/c10:0/c12:0/c14:0中链脂肪酸及其乙酯的生产方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
YUAN L ET AL.: "MODIFICATION OF THE SUBSTRATE SPECIFICITY OF AN ACYL-ACYL CARRIER PROTEIN THIOESTERASE BY PROTEIN ENGINEERING", 《PROC.NATL.ACAD.SCI.USA》 *
元冬娟等: "高等植物的酰基-ACP硫酯酶研究进展", 《中国油料作物学报》 *

Also Published As

Publication number Publication date
BR112015023192A8 (pt) 2018-01-02
AU2018267601A1 (en) 2018-12-06
BR112015023192A2 (pt) 2017-11-21
MX2015011507A (es) 2016-04-07
EP2971024A1 (fr) 2016-01-20
WO2014151904A1 (fr) 2014-09-25
AU2014236763A1 (en) 2015-10-01
EP2971024A4 (fr) 2016-11-16
AU2014236763B2 (en) 2018-08-23
CA2904395A1 (fr) 2014-09-25
JP2016518112A (ja) 2016-06-23
KR20150128770A (ko) 2015-11-18

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