CA2904395A1 - Thioesterases et cellules pour la production d'huiles a facon - Google Patents
Thioesterases et cellules pour la production d'huiles a facon Download PDFInfo
- Publication number
- CA2904395A1 CA2904395A1 CA2904395A CA2904395A CA2904395A1 CA 2904395 A1 CA2904395 A1 CA 2904395A1 CA 2904395 A CA2904395 A CA 2904395A CA 2904395 A CA2904395 A CA 2904395A CA 2904395 A1 CA2904395 A1 CA 2904395A1
- Authority
- CA
- Canada
- Prior art keywords
- seq
- sequence
- oil
- cuphea
- amino acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/02—Thioester hydrolases (3.1.2)
- C12Y301/02014—Oleoyl-[acyl-carrier-protein] hydrolase (3.1.2.14), i.e. ACP-thioesterase
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Nutrition Science (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Fats And Perfumes (AREA)
- Edible Oils And Fats (AREA)
Abstract
La présente invention concerne des gènes de thioestérases acyl-ACP végétales du type FATB et des protéines codées par lesdits gènes. Ces gènes sont utiles pour construire des cellules hôtes de recombinaison présentant des profils d'acide gras modifiés. L'invention concerne également des cellules hôtes de microalgue oléagineuse comportant les nouveaux gènes ou les gènes FATB identifiés précédemment. Les cellules microalgales selon l'invention produisent des triglycérides présentant des profils d'acide gras utiles.
Applications Claiming Priority (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201361791861P | 2013-03-15 | 2013-03-15 | |
US13/837,996 US9290749B2 (en) | 2013-03-15 | 2013-03-15 | Thioesterases and cells for production of tailored oils |
US61/791,861 | 2013-03-15 | ||
US13/837,996 | 2013-03-15 | ||
US201361917217P | 2013-12-17 | 2013-12-17 | |
US61/917,217 | 2013-12-17 | ||
PCT/US2014/026644 WO2014151904A1 (fr) | 2013-03-15 | 2014-03-13 | Thioestérases et cellules pour la production d'huiles à façon |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2904395A1 true CA2904395A1 (fr) | 2014-09-25 |
Family
ID=51581068
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2904395A Abandoned CA2904395A1 (fr) | 2013-03-15 | 2014-03-13 | Thioesterases et cellules pour la production d'huiles a facon |
Country Status (9)
Country | Link |
---|---|
EP (1) | EP2971024A4 (fr) |
JP (1) | JP2016518112A (fr) |
KR (1) | KR20150128770A (fr) |
CN (1) | CN105143458A (fr) |
AU (2) | AU2014236763B2 (fr) |
BR (1) | BR112015023192A8 (fr) |
CA (1) | CA2904395A1 (fr) |
MX (1) | MX2015011507A (fr) |
WO (1) | WO2014151904A1 (fr) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9816079B2 (en) | 2013-01-29 | 2017-11-14 | Terravia Holdings, Inc. | Variant thioesterases and methods of use |
US9567615B2 (en) | 2013-01-29 | 2017-02-14 | Terravia Holdings, Inc. | Variant thioesterases and methods of use |
US9783836B2 (en) | 2013-03-15 | 2017-10-10 | Terravia Holdings, Inc. | Thioesterases and cells for production of tailored oils |
US9290749B2 (en) | 2013-03-15 | 2016-03-22 | Solazyme, Inc. | Thioesterases and cells for production of tailored oils |
BR112017001404A2 (pt) | 2014-07-24 | 2017-11-21 | Terravia Holdings Inc | tioesterases variantes e métodos de utilização |
EP3194582A2 (fr) | 2014-09-18 | 2017-07-26 | TerraVia Holdings, Inc. | Acyl-acp thioestérases et leurs mutants |
US20180142218A1 (en) | 2016-10-05 | 2018-05-24 | Terravia Holdings, Inc. | Novel acyltransferases, variant thioesterases, and uses thereof |
US20230143841A1 (en) | 2020-01-16 | 2023-05-11 | Corbion Biotech, Inc. | Beta-ketoacyl-acp synthase iv variants |
Family Cites Families (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5344771A (en) | 1990-04-26 | 1994-09-06 | Calgene, Inc. | Plant thiosterases |
US5298421A (en) | 1990-04-26 | 1994-03-29 | Calgene, Inc. | Plant medium-chain-preferring acyl-ACP thioesterases and related methods |
US5512482A (en) | 1990-04-26 | 1996-04-30 | Calgene, Inc. | Plant thioesterases |
US5455167A (en) | 1991-05-21 | 1995-10-03 | Calgene Inc. | Medium-chain thioesterases in plants |
US5639790A (en) | 1991-05-21 | 1997-06-17 | Calgene, Inc. | Plant medium-chain thioesterases |
US5850022A (en) | 1992-10-30 | 1998-12-15 | Calgene, Inc. | Production of myristate in plant cells |
US5654495A (en) | 1992-10-30 | 1997-08-05 | Calgene, Inc. | Production of myristate in plant cells |
EP0716708A1 (fr) * | 1993-09-03 | 1996-06-19 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Thioesterases induisant specifiquement des chaines de longueur moyenne |
JPH09505470A (ja) | 1993-11-10 | 1997-06-03 | カルジーン,インコーポレイティド | 植物アシルacpチオエステラーゼ配列 |
US5807893A (en) | 1993-11-18 | 1998-09-15 | Voelker; Toni Alois | Plant thioesterases and use for modification of fatty acid composition in plant seed oils |
EP2351845A1 (fr) | 2007-06-01 | 2011-08-03 | Solazyme, Inc. | Produits chimiques renouvelables et carburants issus de levures oléagineuses |
WO2009007091A2 (fr) * | 2007-07-09 | 2009-01-15 | Bayer Bioscience N.V. | Plante de la famille brassica comprenant des allèles mutants de l'acyle gras-acp thioestérase |
US7982035B2 (en) | 2007-08-27 | 2011-07-19 | Duquesne University Of The Holy Spirit | Tricyclic compounds having antimitotic and/or antitumor activity and methods of use thereof |
DE112009001459T5 (de) * | 2008-06-20 | 2011-09-29 | Basf Plant Science Gmbh | Pflanzen mit gesteigerten ertragsbezogenen Eigenschaften und Verfahren zur Herstellung derselben |
CA2745129C (fr) | 2008-11-28 | 2019-05-07 | Solazyme, Inc. | Production d'huiles personnalisees dans des micro-organismes heterotrophes |
CA2758298A1 (fr) * | 2009-04-10 | 2010-10-14 | Ls9, Inc. | Production de biodiesel commercial a partir de microorganismes genetiquement modifies |
AU2011257982B2 (en) | 2010-05-28 | 2017-05-25 | Corbion Biotech, Inc. | Tailored oils produced from recombinant heterotrophic microorganisms |
KR102117225B1 (ko) | 2011-02-02 | 2020-06-02 | 테라비아 홀딩스 인코포레이티드 | 재조합 유지성 미생물로부터 생산된 맞춤 오일 |
US8951762B2 (en) * | 2011-07-27 | 2015-02-10 | Iowa State University Research Foundation, Inc. | Materials and methods for using an acyl—acyl carrier protein thioesterase and mutants and chimeras thereof in fatty acid synthesis |
CN102586350A (zh) * | 2012-01-09 | 2012-07-18 | 北京化工大学 | 一种c8:0/c10:0/c12:0/c14:0中链脂肪酸及其乙酯的生产方法 |
CA2870364A1 (fr) | 2012-04-18 | 2013-10-24 | Solazyme, Inc. | Microbes recombinants renfermant des enzymes de passage synthetiques d'acides gras modifies |
-
2014
- 2014-03-13 BR BR112015023192A patent/BR112015023192A8/pt not_active Application Discontinuation
- 2014-03-13 AU AU2014236763A patent/AU2014236763B2/en not_active Ceased
- 2014-03-13 CN CN201480020002.5A patent/CN105143458A/zh active Pending
- 2014-03-13 JP JP2016502205A patent/JP2016518112A/ja active Pending
- 2014-03-13 WO PCT/US2014/026644 patent/WO2014151904A1/fr active Application Filing
- 2014-03-13 MX MX2015011507A patent/MX2015011507A/es unknown
- 2014-03-13 KR KR1020157027058A patent/KR20150128770A/ko not_active Application Discontinuation
- 2014-03-13 CA CA2904395A patent/CA2904395A1/fr not_active Abandoned
- 2014-03-13 EP EP14769502.7A patent/EP2971024A4/fr not_active Withdrawn
-
2018
- 2018-11-21 AU AU2018267601A patent/AU2018267601A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
WO2014151904A1 (fr) | 2014-09-25 |
CN105143458A (zh) | 2015-12-09 |
BR112015023192A8 (pt) | 2018-01-02 |
BR112015023192A2 (pt) | 2017-11-21 |
AU2014236763A1 (en) | 2015-10-01 |
EP2971024A4 (fr) | 2016-11-16 |
EP2971024A1 (fr) | 2016-01-20 |
MX2015011507A (es) | 2016-04-07 |
KR20150128770A (ko) | 2015-11-18 |
JP2016518112A (ja) | 2016-06-23 |
AU2014236763B2 (en) | 2018-08-23 |
AU2018267601A1 (en) | 2018-12-06 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request |
Effective date: 20190312 |
|
FZDE | Discontinued |
Effective date: 20210831 |