CN105123523B - Blbizzia falcata woods select tree rapid seedling cultivation technology - Google Patents
Blbizzia falcata woods select tree rapid seedling cultivation technology Download PDFInfo
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Abstract
The invention discloses a kind of method of blbizzia falcata woods select tree rapid seedling cultivation, the method is inoculated in clump bud inducement cultivation base as explant, after sterilization with select tree rudiment bar in woods and induces clump bud；Clump bud is inoculated in squamous subculture in subculture multiplication medium again；Then being inoculated in root media carries out culture of rootage, and hardening and field planting management are carried out after taking root.In the present invention; by adjusting a great number of elements, hormone combination and concentration etc., propagation seedling quality and tissue-cultured seedling rooting rate are improve, the perfect tissue culture technological process of production, reduce production cost; improve production efficiency, realizes the industrialization of blbizzia falcata choiceness, large-scale production.
Description
Technical field
The present invention relates to select tree seed selection in blbizzia falcata breeding method, particularly blbizzia falcata woods and rapid seedling cultivation technical method.
Belong to the fine-variety breeding and seedling propagation technical field of tropical forest particularly blbizzia falcata.
Background technology
Blbizzia falcata originates in Southeast Asia, is world-renowned tropical fast-growing commerical tree species, be born with 5~6 years can final felling utilize,
Indonesia's increment is up to 103~108m3/hm2Year.Blbizzia falcata root nodule is abundant, it is many, perishable to fall leaves, and is improved soil
Fine tree species.Timber is the good raw material of paper industry and wood-based panel industry, thus is drawn extensively in the torrid zone, South Subtropical Area of China
Plant cultivation.
The provinces and regions such as China Guangdong, Guangxi, Hainan, Fujian, Taiwan extensive introducing and plantings blbizzia falcata.It is good in land occupation condition
Location, diameter of a cross-section of a tree trunk 1.3 meters above the ground year increment reaches 8cm, 2~3 times faster than eucalyptus of the speed of growth.Blbizzia falcata adaptability is also stronger, in average annual temperature
20~28 DEG C, the area energy well-grown of extremely minimum 2~-2 DEG C, 1300~2000mm of annual rainfall, in China
The torrid zone, South Subtropical Area of China have huge development potentiality.
Traditional blbizzia falcata is afforested based on seedling forestation, not only the growth indexes such as the height of tree, the diameter of a cross-section of a tree trunk 1.3 meters above the ground, volume of timber differentiation pole
Greatly, and the Tree form indexes such as branch quantity, branch thickness, clear bole height, hat width, taperingness, material also break up huge, influence standing forest life
Amount long and increase forest management difficulty, meanwhile, seedling forestation, the forest for having 50% or so produces disease, and 20-30% forests can disease
Extremely, it is necessary to overcome this disadvantage by improving the density of plantation, cost of forestation is increased.At present, blbizzia falcata tissue culture at home is ground
Study carefully in report, explant used has the seedling after seed disinfection, 2~3 years raw select tree grafting coppice shoots in nursery, obtains again
Raw plant, but have not seen the report of factorial praluction and full wafer afforestation.
In the blbizzia falcata woods of large area, select tree is selected with the high intensity of 1/3000-5000, then by Clones of Plus Trees,
The cultivation road of asxualization is walked, stand growth, uniformity, form, material, winter resistance and barren-resistant property etc. is significantly improved,
It is reliability, the quick method of blbizzia falcata fine-variety breeding and nursery stock industrial fast breeding.The rapid seedling cultivation of select tree in research and development blbizzia falcata woods
Technology has great economy and realistic meaning.
The content of the invention
It is an object of the invention to provide a kind of method of blbizzia falcata woods select tree rapid seedling cultivation.
The technical solution used in the present invention is：
A kind of method of blbizzia falcata woods select tree rapid seedling cultivation, including following operating procedure：
1）The collection of branch：
In the fall, the height by the select tree taken out from blbizzia falcata Lin Zhongxuan apart from 20~50cm of ground is cut down, secondary
The branch of year new rudiment gathers branch when being up to 45 ~ 55cm；
2）The clip of branch and sterilization：
The branch of collection is carried out disinfection, branch is cut at least one axillary bud, length of band on superclean bench after sterilization
The explant for 1.5~2.5cm is spent, and petiole length is trimmed to 0.4~0.6cm；
3）Clump bud Fiber differentiation and shoot proliferation culture：
The above-mentioned explant for disinfecting is inoculated in clump bud inducement cultivation base, induction clump bud is sprouted, clump bud germination and growth
When highly reaching more than 2cm, being transferred in subculture multiplication medium carries out shoot proliferation culture；
4）Culture of rootage：Clump bud in the subculture multiplication medium after culture 25~30 days, a height of 2.0~3.0cm of clip and
With at least 2 terminal buds for being fully deployed compound leaf, induced bud seedling rooting in root media of transferring；
5）Practice seedling：When culture of rootage sprout rooting rate is up to more than 80%, carry out practicing seedling, practice the seedling time for 14~21 days；According to
Practice the seedling time according to different season control, the high temperature season white silk seedling time is short, and it is long that low temperature season practices the seedling time.
6）Outplanting：Sprout of taking root through practicing after seedling carries out outplanting.
Further, step 1）The choosing method of the select tree is：In blbizzia falcata artificial forest, by 1/5000~1/3000
Selection intensity, using five plants of select trees wood methods, select tree is selected in Comprehensive Assessment.
Further, step 1）Positive day sunny after selection at 10 points in the morning, after collection, cuts branch during the collection branch
1/2 ~ 5/6 blade, and tied with the encirclement of paper, film or cloth, to keep branch moisture in transit.
Further, step 2）Described in same day collection branch same day sterilization, concrete operations are：Branch blade is cut off, is protected
Petiole 0.5 ~ 1.5cm of length is stayed, branch is cleaned with detergent, and rinsed with clear water；Again on superclean bench, by above-mentioned branch
From terminal bud down, 3~4 sections are cut to, every section at least retains an axillary bud, respectively with mercuric chloride 8~30min of sterilization, then use sterilized water
Clean up.
Further, step 3）Middle clump bud inducement cultivation base is to contain 0.5 ~ 0.8mg/L 6-BA, 0.2 ~ 0.5mg/L
The modified MS medium of NAA, 0. 40~0.60 times of normal MS a great number of elements content.
Further, step 3）Middle subculture multiplication medium is to contain 0.1~0.3mg/L6-BA, 0.10 ~ 0.3mg/
LNAA, KNO3、NH4NO3、CaCl2Content be respectively 0.6~0.85 times of normal MS, 0.5~0.7 times, 1.1~1.3 times
Modified MS medium.
Further, step 4）Middle root media is to contain 0.10 ~ 0.20mg/L NAA, 0.15 ~ 0.25mg/L
IBA、325~385mg/L Ca(NO3)2, 18~22g/L sucrose modified MS medium.
Further, step 5）Light intensity during middle experienced seedling is 5000 ~ 8000Lx, temperature is 15 ~ 38 DEG C；Practice the seedling time be 2 ~
May or 9 ~ November.
Further, step 6）The time of middle transplanting is 2~June or 9~November, when transplant time is 2~May,
The period, rainfall is plentiful, and air humidity is big, with yellow soil and Light media volume ratio（6.0~8.0）：（2.0~4.0）It is transplanting base
Matter, with true yellow cubsoil as grafting matrix when other months transplant.
Further, step 6）Middle outplanting and management concrete operations are：Cover film and shading screen, initial stage after transplanting
It is 90~95% in sunshade rate, relative humidity more than 95%, day temperature thereafter will to be cultivated under conditions of 22~35 DEG C 6~9 days
Sunshade rate, relative humidity are reduced to 80~90% continuation and cultivate 4~7 days respectively, then gradually reduce relative humidity extremely in 5~7 days
Removal film, then sunshade rate is gradually reduced to removing, by nursery stock culture up to more than 25cm under natural conditions.
The beneficial effects of the invention are as follows：
1st, the present invention presses 1/5000~1/3000 selection intensity, and using five plants of select trees wood methods, select tree is selected in Comprehensive Assessment,
Overcome because select superior tree intensity is too low, select excellent precision problem not high or under-represented.
2nd, in the present invention, bar tissue-culturing rapid propagation nursery is adopted to select tree in woods, the intermediate link of grafting is reduced, is not only shortened
Choiceness incubation time, while during reducing choiceness, because culture link is excessive, causing choiceness
The risk of loss.
3rd, in the present invention, by adjusting KNO3、NH4NO3、CaCl2、Ca(NO3)2Etc. concentration, propagation nursery stock is improved
Matter degree, optimizes seedling quality, improves productivity effect.By adjusting NAA, IBA, Ca (NO3)2, the concentration such as sucrose, really
Determine cultivation temperature, improve tissue-cultured seedling rooting rate.The present invention improves propagation seedling by adjusting a great number of elements, hormone combination and concentration
Wooden amount and tissue-cultured seedling rooting rate, improve the tissue culture technological process of production, reduce production cost, and improve production efficiency realizes Nan Yang
The industrialization of principal columns of a hall choiceness, large-scale production.
5th, the perfect tissue culture technological process of production of the present invention, realize the industrialization of blbizzia falcata choiceness, large-scale production.
The present invention obtains regeneration plant using forest land select tree coppice shoot, has both maintained the merit of original select tree, and grafting has been saved again
Intermediate link, not only shortens choiceness incubation time, while because of culture link during reducing choiceness
Excessively, the risk for causing choiceness to lose.
6th, by select tree rapid seedling cultivation technical method in blbizzia falcata woods of the present invention, quickly breeding clone nursery stock out,
Overcome the growth indexes differentiation such as the height of tree, the diameter of a cross-section of a tree trunk 1.3 meters above the ground, volume of timber that existing seminal propagation method is present very big；Branch quantity, branch thickness,
The differentiation of the Tree form indexes such as clear bole height, hat width, taperingness, material is also huge；Also difference is huge etc. lacks for disease-resistant, cold-resistant, the fertile degree of happiness
Point, there is provided form is logical straight, disease-resistant, cold-resistant, the choiceness blbizzia falcata nursery stock of fast-growing, not only improves blbizzia falcata Stand Growth
Amount, while improving Stand Growth quality, reduces the density of plantation, reduces the afforestation expenses of blbizzia falcata, realizes industrialization production.
7th, the present invention new selection method and new tissue culture technology means overcome the height of tree that existing seminal propagation method is present,
The differentiation of the growth indexes such as the diameter of a cross-section of a tree trunk 1.3 meters above the ground, the volume of timber is very big；The form quality such as branch quantity, branch thickness, clear bole height, hat width, taperingness, material refer to
Mark differentiation is also huge；Disease-resistant, cold-resistant, the fertile degree of happiness be the shortcomings of also difference is huge, and providing form within the most short most fast time leads to
Directly, select tree rapid seedling cultivation technology in disease-resistant, cold-resistant, the blbizzia falcata woods of fast-growing.25 blbizzia falcata clonal tissue cultures are produced at present
Seedling, there is provided 100,000 plants of nursery stock of afforestation, nearly thousand mu of afforestation area.
Brief description of the drawings
Fig. 1 is the secondary blbizzia falcata woods for choosing select tree；
Fig. 2 is the select tree and new coppice shoot long after felling；
Fig. 3 is the select tree and new coppice shoot long after felling；
Fig. 4 is the explant in sterilization；
Fig. 5 is that the explant after inoculation sterilization enters the clump bud induction initial stage；
Fig. 6 is 2 plants of situations of clump bud induction, after 2 explants were through clump bud Fiber differentiation 1-2 month, grows clump bud, high
More than 2cm, preparation is transferred to Multiplying culture；
Fig. 7 is the Regenerated plant after proliferation and subculture culture 25-30 days；
Fig. 8 goes out root situation for culture of rootage after 14 days；
Fig. 9 goes out root situation for culture of rootage after 14 days；
Figure 10 is that seedling figure is practiced in greenhouse, and practicing seedling can transplant after 14 ~ 21 days；According to seasonal variations, summer temperature heat, when practicing seedling
Between it is short, winter temperature is low, practice the seedling time it is long；
Figure 11 is Nursery stock transplanting figure；
Figure 12 is the growing state of nursery stock after transplanting month；
Figure 13 is to transplant the nursery stock after 2 first quarter moons, can go out garden.
Specific embodiment
A kind of method of blbizzia falcata woods select tree rapid seedling cultivation, including following operating procedure：
1）The collection of branch：
In the fall, the height by the select tree taken out from blbizzia falcata Lin Zhongxuan apart from 20~50cm of ground is cut down, secondary
The branch of year new rudiment gathers branch when being up to 45 ~ 55cm.
2）The clip of branch and sterilization：
The branch of collection is carried out disinfection, branch is cut at least one axillary bud, length of band on superclean bench after sterilization
The explant for 1.5~2.5cm is spent, and petiole length is trimmed to 0.4~0.6cm.
3）Clump bud Fiber differentiation and shoot proliferation culture：
The above-mentioned explant for disinfecting is inoculated in clump bud inducement cultivation base, induction clump bud is sprouted, clump bud germination and growth
When highly reaching more than 2cm, being transferred in subculture multiplication medium carries out shoot proliferation culture；
4）Culture of rootage：Clump bud in the subculture multiplication medium after culture 25~30 days, a height of 2.0~3.0cm of clip and
With at least 2 terminal buds for being fully deployed compound leaf, induced bud seedling rooting in root media of transferring；
5）Practice seedling：When culture of rootage sprout rooting rate is up to more than 80%, carry out practicing seedling, practice the seedling time for 14~21 days.
6）Outplanting：Sprout of taking root through practicing after seedling carries out outplanting and management.
Preferably, step 1）The choosing method of the select tree is：In blbizzia falcata artificial forest, by 1/5000~1/3000
Selection intensity, using five plants of select tree wood methods, select tree is selected in Comprehensive Assessment.
Preferably, step 1）During the felling, it is ensured that otch is smoothed, it is to avoid the stake of lacerated wound select tree, rudiment is influenceed；Simultaneously excellent
Shrub, weeds are cleaned out in 3~5m radius of tree, the upper strata forest of periphery sunshade is cut down, increases light transmittance, promoted excellent
Tree rudiment, healthy growth.
Preferably, step 1）During middle collection branch, the upper part of clip branch, end portion is left to few 2 pairs of axillary buds, 9
Stop collection branch after month.
Preferably, step 1）Positive day sunny after selection at 10 points in the morning, after collection, cuts branch 1/ during the collection branch
2 ~ 5/6 blade, and tied with the encirclement of paper, film or cloth, to keep branch moisture in transit.
Preferably, step 2）Described in same day collection branch same day sterilization, concrete operations are：Branch blade is cut off, is retained
Petiole 0.5 ~ 1.5cm of length, cleans branch, and rinsed with clear water with detergent；Again on superclean bench, by above-mentioned branch from
Terminal bud down, is cut to 3~4 sections, and every section at least retains an axillary bud, is sterilized 8~30min with 1g/L mercuric chloride respectively, then with aseptic
Water is cleaned up.
Preferably, step 3）Middle clump bud inducement cultivation base be containing 0.5 ~ 0.8mg/L 6-BA, 0.2 ~ 0.5mg/L NAA,
0. 40~0.60 times of modified MS medium of normal MS a great number of elements contents.
Preferably, step 3）The condition of middle clump bud Fiber differentiation is：1500~3000Lx of intensity of illumination, temperature 22~28
℃。
Preferably, step 3）Middle subculture multiplication medium be containing 0.1~0.3mg/L6-BA, 0.10 ~ 0.3mg/LNAA,
KNO3、NH4NO3、CaCl2Content be respectively 0.6~0.85 times of normal MS, 0.5~0.7 times, 1.1~1.3 times of improvement MS
Culture medium.
Preferably, step 3）Middle subculture Multiplying culture and step 4）The condition of middle culture of rootage is：Intensity of illumination 2000 ~
3000Lx, temperature are 20~28 DEG C.
Preferably, step 4）Middle root media be containing 0.10 ~ 0.20mg/L NAA, 0.15 ~ 0.25mg/L IBA,
325~385mg/L Ca(NO3)2, 18~22g/L sucrose modified MS medium.
Preferably, step 5）Light intensity during middle experienced seedling is 5000 ~ 8000Lx, temperature is 15 ~ 38 DEG C；The white silk seedling time is 2 ~ 5
The moon or 9 ~ November.
Preferably, step 6）The time of middle transplanting is 2~June or 9~November, when transplant time is 2~May, should
Period, rainfall is plentiful, and air humidity is big, with yellow soil and Light media volume ratio（6.0~8.0）：（2.0~4.0）It is grafting matrix,
With true yellow cubsoil as grafting matrix when transplanting in other months.
Preferably, above-mentioned grafting matrix be with the sterilized grafting matrix of 2/1000 ~ 4/1000 liquor potassic permanganate, and
1/1000 Bravo, 1/1000 carbendazim per week are sprayed in turn after the transfer.
Preferably, step 6）Middle outplanting and management concrete operations are：Cover film and shading screen after transplanting, the initial stage exist
Sunshade rate is 90~95%, relative humidity more than 95%, and day temperature will hide thereafter to be cultivated under conditions of 22~35 DEG C 6~9 days
Positive rate, relative humidity are reduced to 80~90% continuation and cultivate 4~7 days respectively, and relative humidity was then gradually reduced in 5~7 days to going
Except film, then sunshade rate is gradually reduced to removing, by nursery stock culture up to more than 25cm under natural conditions.
Preferably, the fertilizing method after outplanting is to be sprayed weekly once with 1/1000 composite fertilizer's solution.
With reference to specific embodiment, the present invention is further illustrated, but is not limited thereto.
Select tree rapid seedling cultivation technology in the blbizzia falcata woods of embodiment 1
The present embodiment is secondary 20 plants of the blbizzia falcata Lin Zhongxuan select tree in Boluo field Liao Bei forest farms in 2009, select tree germination rate
50%, explant sterilizes breeding success rate up to 80%, there is provided 8 clonal test seedlings.And select disease-resistant variety TL17 at present
Popularization and application, it is deep to be liked by forest zone self-employed tree cultivator.Specific operation process is as follows：
1）The collection of branch
1. the selection of select tree：
In the blbizzia falcata scondary forest in Boluo field Liao Bei forest farms（As shown in Figure 1）In, by 1/5000~1/3000 selection intensity,
Using five plants of select tree wood methods, 20 plants of select tree is selected in Comprehensive Assessment；
The concrete operations of wherein five plants select trees wood methods are：Centered on candidate select tree, in the range of 10 meters, 5 plants of secondary are surveyed
The diameter of a cross-section of a tree trunk 1.3 meters above the ground of select tree, the height of tree, calculate mean DBH increment, the mean stand height of secondary select tree.With the diameter of a cross-section of a tree trunk 1.3 meters above the ground of candidate select tree, the height of tree and secondary
The average value of select tree is compared, and the candidate tree that the average value 10%, height of tree exceedes average value 15% is exceeded to the diameter of a cross-section of a tree trunk 1.3 meters above the ground, enters to elect primary election as
Select tree.The classification marking such as the diameter of a cross-section of a tree trunk 1.3 meters above the ground, the height of tree, branch, logical straight degree, clear bole height, garden full scale, hat width to primary election select tree, it is last comprehensive
Select tree is selected in evaluation.
2. the felling of select tree：
In the fall（October）, apart from the height felling select tree of 20~50cm of ground or so（As shown in Figures 2 and 3）, cut
When cutting down, notice that otch is smoothed, it is to avoid the lacerated wound select tree village, influence rudiment；Cleaned out in 3~5m radius of select tree simultaneously
Shrub, weeds, cut down the upper strata forest of periphery sunshade, increase light transmittance, promote select tree rudiment, healthy growth.
3. bar is adopted：
In next year 3, April, the select tree cut down starts rudiment（As shown in Figures 2 and 3）, rudiment is up to 50cm or so and opens
Begin to adopt bar, when adopting bar, the upper part of clip branch leaves at least 2 pairs axillary buds to branch end portion, stops to adopt after September,
Allow select tree village restoration ecosystem, winterization；
Wherein, positive day sunny after selection at 10 points in morning when adopting bar（Bar is adopted in the positive day of 2 ~ 3 days preferably after rain）, collection
Afterwards, the blade of branch 2/3 is cut, and is tied with the encirclement of paper, film or cloth, to keep branch moisture, and be readily transported.
2）The editing and sterilization of branch
The branch of collection is carried out disinfection, the branch of collection should day sterilize in time as early as possible；
The concrete operations of sterilization are：Blade is cut off, retains 0.5 ~ 1.5cm petioles long, cleaned branch, clear water with detergent and rush
Washing carries out pre-sterilization；Again on superclean bench, by pre-pasteurized branch from terminal bud down, be cut into 3~4 by degree of lignification
Section, every section at least retains an axillary bud（As shown in Figure 4）, respectively with 1g/L mercuric chloride 8~30min of sterilization, sterilized water is used after sterilization
Soaking flushing 3 ~ 5 times, soaks 5 minutes every time, you can；
The branch that taking-up is disinfected, aseptically, 1.5~2.5cm explants long, each explant is cut into by branch
Body at least retains an axillary bud, and the petiole of explant is trimmed to 0.4~0.6cm length（As shown in Figure 5）.
3）Clump bud Fiber differentiation and shoot proliferation culture
Clump bud Fiber differentiation and Multiplying culture：By above-mentioned sterilization, simultaneously the good explant of editing is inoculated in clump bud Fiber differentiation
（As shown in Figure 5）, it is 1500-3000Lx in intensity of illumination, under room temperature is for 23-28 DEG C of condition of culture, cultivate 20-30 days, clump
Bud starts to sprout；
When the clump bud sprouted grows to more than 2cm（Fig. 6）, it is transferred to squamous subculture in subculture multiplication medium, subculture
The condition of Multiplying culture is：20~28 DEG C of intensity of illumination 2000-3000Lx, room temperature, cultivates 25~30 days, and proliferation times are 2.0
~3.0 times（Such as Fig. 7）.
Above-mentioned clump bud inducement cultivation base is：Hormone concentration containing 0.5 ~ 0.8mg/L 6-BA and 0.2 ~ 0.5mg/L NAA
MS culture mediums, but its a great number of elements content is 0. 40~0.60 times of normal MS.
Above-mentioned subculture multiplication medium is：MS culture mediums containing 0.1~0.3mg/L6-BA and 0.10 ~ 0.3mg/L, but
Its KNO3、NH4NO3、CaCl2Content be respectively 0.8 times, 0.6 times, 1.2 times of normal MS.
4）Culture of rootage
After clump bud is cultivated 25~30 days in subculture multiplication medium（Fig. 7）, a height of 2.0~3.0cm of clip and with extremely
Few 2 terminal buds for being fully deployed compound leaf, root induction in root media of transferring, culture of rootage condition and squamous subculture condition
Identical, culture of rootage goes out root situation as shown in Figure 8, Figure 9 after 14 days.
Above-mentioned root media is：It is to contain 0.10 ~ 0.20mg/L NAA, 0.15-0. 25mg/L IBA, 350mg/L
Ca(NO3)2, 20g/L sucrose MS culture mediums.
5）Practice seedling before transplanting
After culture of rootage sprout rooting rate is up to more than 80%, moves to experienced seedling room and practice seedling（As shown in Figure 10）, practice seedling room temperature
It is 15 ~ 38 DEG C of natural temperatures, illumination is by force 5000 ~ 8000Lx, notices that cold wave heating is aeration-cooling with summer, practices seedling time length
Become because of season, the summer manner time high long autumn manner low time is short, is 14 ~ 21 days, when seedling growth height up to 2.5~
6.0cm and the compound leaf with more than two panels being fully deployed or when starting radical leaves occur to change into yellow leaf, completing to practice seedling carries out field
Transplanting.
6）Outplanting
The Nursery stock transplanting of after seedling will be practiced in grafting matrix, field culture will be carried out（As shown in figure 11）.The tool of field culture
Gymnastics conduct：
Cover film and shading screen after transplanting, first by nursery stock sunshade rate be 90~95%, relative humidity more than 95%, daytime
Temperature is culture 7 days under conditions of 22~35 DEG C；Then sunshade rate, relative humidity will be put and will be reduced to 80~90% respectively, temperature will be 22
Cultivated 4~7 days under conditions of~35 DEG C；Then relative humidity was gradually reduced in 5~7 days to removing film, then is gradually reduced
Sunshade rate cultivates up to more than 25cm nursery stock under natural conditions to removing, you can go out garden afforestation.
The present embodiment transplant one month after nursery stock growing state as shown in figure 12, transplanting 2 first quarter moons after nursery stock as scheme
Shown in 13, nursery stock is up to more than 25cm, and nursery stock now can go out garden afforestation.
The present embodiment 1 is taken root bottle seedling transplanting success up to more than 95%, and outplanting rate is up to more than 85%.
The time of above-mentioned outplanting is 2~June or 9~November；If empty because rainfall is plentiful in the transplanting of 2~May
Gas high humidity, with yellow soil and other Light media volume ratios（6.0~8.0）：（2.0~4.0）It is grafting matrix, the transplanting of other months
When with true yellow cubsoil as grafting matrix.Used grafting matrix need to be sterilized with 2/1000 ~ 4/1000 liquor potassic permanganate,
And 1/1000 Bravo, 1/1000 carbendazim per week are sprayed in turn after the transfer.
Fertilizing method after above-mentioned outplanting is and the composite fertilizer's solution with 1/1000 is sprayed weekly once.
Select tree rapid seedling cultivation technology in the blbizzia falcata woods of embodiment 2
The present embodiment selected 9 plants of cold-resistant select tree for 2010 in the secondary Nan Yang Lin Zhongxuan in Zengcheng Chi Ling villages, specifically operated
Journey is with embodiment 1.7 plants of rudiment in 9 plants of select trees, 100% fast numerous success successfully obtains clonal test seedling, and successfully popularization should
With, cold-resistant kind has been selected at present, and in popularization and application.
1）The collection of branch
1. the selection of select tree：
In the blbizzia falcata scondary forest in Zengcheng Chi Ling villages, by 1/5000 selection intensity, using five plants of select tree wood methods, comprehensively comment
Surely 9 plants of select tree is selected；
The concrete operations of wherein five plants select trees wood methods are：Centered on candidate select tree, in the range of 10 meters, 5 plants of secondary are surveyed
The diameter of a cross-section of a tree trunk 1.3 meters above the ground of select tree, the height of tree, calculate mean DBH increment, the mean stand height of secondary select tree.With the diameter of a cross-section of a tree trunk 1.3 meters above the ground of candidate select tree, the height of tree and secondary
The average value of select tree is compared, and the candidate tree that the average value 10%, height of tree exceedes average value 15% is exceeded to the diameter of a cross-section of a tree trunk 1.3 meters above the ground, enters to elect primary election as
Select tree.The classification marking such as the diameter of a cross-section of a tree trunk 1.3 meters above the ground, the height of tree, branch, logical straight degree, clear bole height, garden full scale, hat width to primary election select tree, it is last comprehensive
Select tree is selected in evaluation.
2. the felling of select tree：
In the fall（October）, the height apart from ground 50cm cuts down select tree, during felling, notices that otch is smoothed, it is to avoid split
Hinder the select tree village, influence rudiment；Shrub, weeds are cleaned out in the 4m radius of select tree simultaneously, the upper of periphery sunshade is cut down
Row upon row of trees wood, increases light transmittance, promotes select tree rudiment, healthy growth.
3. bar is adopted：
In next year 3, April, the select tree cut down starts rudiment, and rudiment is up to 50cm or so and starts that bar can be adopted, and adopts bar
When, the upper part of clip branch leaves at least 2 pairs axillary buds to branch end portion, stops to adopt after September, allows the select tree village to recover life
It is long, winterization；
Wherein, positive day sunny after selection at 10 points in morning when adopting bar（Bar is adopted in the positive day of 2 ~ 3 days preferably after rain）, collection
Afterwards, the blade of branch 2/3 is cut, and is tied with the encirclement of paper, film or cloth, to keep branch moisture, and be readily transported.
2）The editing and sterilization of branch
The branch of collection is carried out disinfection, the branch of collection should day sterilize in time as early as possible；
The concrete operations of sterilization are：Blade is cut off, retains 1cm petioles long, branch, clear water flushing are cleaned with detergent to be carried out
Pre-sterilization；Again on superclean bench, by pre-pasteurized branch from terminal bud down, be cut into 3~4 sections by degree of lignification, often
Section at least retains an axillary bud, respectively with 1g/L mercuric chloride 8~30min of sterilization, uses sterilized water soaking flushing 3 ~ 5 times after sterilization, often
Secondary immersion 5 minutes, you can；
The branch that taking-up is disinfected, aseptically, 2cm explants long is cut into by branch, and each explant is at least
Retain an axillary bud, the petiole of explant is trimmed to 0.5cm length.
3）Clump bud Fiber differentiation and shoot proliferation culture
Clump bud Fiber differentiation and Multiplying culture：By above-mentioned sterilization, simultaneously the good explant of editing is inoculated in clump bud Fiber differentiation
In, it is 2500Lx in intensity of illumination, under room temperature is 26 DEG C of condition of culture, to cultivate 25 days, clump bud starts to sprout；
When the clump bud sprouted grows to more than 2cm, squamous subculture in subculture multiplication medium, shoot proliferation training are transferred to
Foster condition is：26 DEG C of intensity of illumination 2500Lx, room temperature, cultivates 25~30 days, and proliferation times are 2.0~3.0 times.
Above-mentioned clump bud inducement cultivation base is：Hormone concentration MS culture mediums containing 0.5mg/L 6-BA and 0.2mg/L NAA,
But its a great number of elements content is 0. 5 times of normal MS.
Above-mentioned subculture multiplication medium is：MS culture mediums containing 0.2mg/L6-BA and 0.10 ~ 0.3mg/LNAA, but its
KNO3、NH4NO3、CaCl2Content be respectively 0.8 times, 0.6 times, 1.2 times of normal MS.
4）Culture of rootage
Clump bud in the subculture multiplication medium after culture 25~30 days, a height of 2.0~3.0cm of clip and with least 2
The terminal bud of compound leaf is fully deployed, root induction in root media of transferring, culture of rootage condition is identical with squamous subculture condition.
Above-mentioned root media is：It is to contain 0.10 ~ 0.20mg/L NAA, 0.15-0. 25mg/L IBA, 350mg/L
Ca(NO3)2, 20g/L sucrose MS culture mediums.
5）Practice seedling before transplanting
After culture of rootage sprout rooting rate is up to more than 80%, moves to experienced seedling room and practice seedling, it is 15 ~ 38 DEG C of natures to practice seedling room temperature
Temperature, illumination is by force 5000 ~ 8000Lx, notices that cold wave heating is aeration-cooling with summer, and white silk seedling time length becomes because of season, the autumn
The summer manner time high long manner low time in season is short, is 14 ~ 21 days, when seedling growth height up to 2.5~6.0cm and band two panels with
On the compound leaf that is fully deployed or when starting radical leaves occur to change into yellow leaf, completing to practice seedling carries out outplanting.
6）Outplanting
The Nursery stock transplanting of after seedling will be practiced in grafting matrix, field culture will be carried out.Field culture concrete operations be：
Cover film and shading screen after transplanting, first by nursery stock sunshade rate be 90~95%, relative humidity more than 95%, daytime
Temperature is culture 7 days under conditions of 22~35 DEG C；Then sunshade rate, relative humidity will be put and will be reduced to 80~90% respectively, temperature will be 22
Cultivated 6 days under conditions of~35 DEG C；Then relative humidity was gradually reduced in 6 days to removing film, then gradually reduces sunshade rate
To removing, by nursery stock culture up to more than 25cm under natural conditions, you can go out garden afforestation.
The present embodiment transplant one month after nursery stock growing state as shown in figure 12, transplanting 2 first quarter moons after nursery stock be up to
More than 25cm, nursery stock now can go out garden afforestation.
The time of above-mentioned outplanting is 2~June or 9~November；If empty because rainfall is plentiful in the transplanting of 2~May
Gas high humidity, with yellow soil and other Light media volume ratios（6.0~8.0）：（2.0~4.0）It is grafting matrix, the transplanting of other months
When with true yellow cubsoil as grafting matrix.Used grafting matrix need to be sterilized with 2/1000 ~ 4/1000 liquor potassic permanganate,
And 1/1000 Bravo, 1/1000 carbendazim per week are sprayed in turn after the transfer.
Fertilizing method after above-mentioned outplanting is and the composite fertilizer's solution with 1/1000 is sprayed weekly once.
Select tree rapid seedling cultivation technology in the blbizzia falcata woods of embodiment 3
The present embodiment is that the blbizzia falcata of the fast-growing under the conditions of the secondary Nan Yang woods selection medium fertility of Huidong all sorts of flowers in 2010 is excellent
Tree, specific operating process is with embodiment 1.Experimental result equally also have successfully been obtained clonal test seedling, have selected fast-growing
Choiceness B7.
Select tree rapid seedling cultivation technology in the blbizzia falcata woods of embodiment 4
The present embodiment is that the blbizzia falcata of the fast-growing under the conditions of the secondary Nan Yang woods selection medium fertility of Huidong all sorts of flowers in 2010 is excellent
Tree, specific operating process is with embodiment 1.Experimental result equally also have successfully been obtained clonal test seedling, select fast-growing
Choiceness is big by 2.
Select tree rapid seedling cultivation technology in the blbizzia falcata woods of embodiment 5
The present embodiment is to select medium fertility with select tree on mountain top, ridge line in the secondary Nan Yang Lin Zhongxuan in Huidong Baima in 2010
20 plants, specific operating process is with embodiment 1.Experimental result shows more than 80% rudiment nursery success, successfully obtains clone examination
Test and use seedling, and success popularization and application.
Select tree rapid seedling cultivation technology in the blbizzia falcata woods of embodiment 6
The present embodiment is selected 8 plants of cold-resistant select tree and was specifically operated in September, 2011 in gantry sand secondary Nan Yang Lin Zhongxuans of Jing
Journey is with embodiment 1.Experimental result shows 8 plants of 5 plants of select tree rudiments, rudiment 100% fast numerous success of strain, and the seedling that will have been obtained
Wood is afforested, and successfully obtains clonal test woods.
Above-described embodiment is the present invention preferably implementation method, but embodiments of the present invention are not by above-described embodiment
Limitation, it is other it is any without departing from Spirit Essence of the invention and the change, modification, replacement made under principle, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (6)
1. a kind of method of blbizzia falcata woods select tree rapid seedling cultivation, it is characterised in that：Including following operating procedure：
1）The collection of branch：
In the fall, the height by the select tree taken out from blbizzia falcata Lin Zhongxuan apart from 20~50cm of ground is cut down, and next year is new
The branch of rudiment gathers branch when being up to 45 ~ 55cm；
2）The clip of branch and sterilization：
The branch of collection is carried out disinfection, branch is cut into at least one axillary bud, length of band on superclean bench after sterilization is
The explant of 1.5~2.5cm, and petiole length is trimmed to 0.4~0.6cm；
3）Clump bud Fiber differentiation and shoot proliferation culture：
The above-mentioned explant for disinfecting is inoculated in clump bud inducement cultivation base, induction clump bud is sprouted, and clump bud germination and growth is highly
During up to more than 2cm, being transferred in subculture multiplication medium carries out shoot proliferation culture；
4）Culture of rootage：Clump bud a height of 2.0~3.0cm of clip and is carried in the subculture multiplication medium after culture 25~30 days
At least 2 terminal buds for being fully deployed compound leaf, induced bud seedling rooting in root media of transferring；
5）Practice seedling：When culture of rootage sprout rooting rate is up to more than 80%, carry out practicing seedling, practice the seedling time for 14~21 days；
6）Outplanting：Sprout of taking root through practicing after seedling carries out outplanting；
Step 3）Middle clump bud inducement cultivation base is to contain 0.5 ~ 0.8mg/L 6-BA, 0.2 ~ 0.5mg/L NAA, 0. 40~0.60
The modified MS medium of times normal MS a great number of elements contents；
Step 3）Middle subculture multiplication medium is to contain 0.1~0.3mg/L6-BA, 0.10 ~ 0.3mg/LNAA, KNO3、NH4NO3、
CaCl2Content be respectively 0.6~0.85 times of normal MS, 0.5~0.7 times, 1.1~1.3 times of modified MS medium；
Step 4）Middle root media is to contain 0.10 ~ 0.20mg/L NAA, 0.15 ~ 0.25mg/L IBA, 325 ~ 385mg/L
Ca(NO3)2, 18~22g/L sucrose MS culture mediums；
Step 1）The choosing method of the select tree is：In blbizzia falcata artificial forest, by 1/5000~1/3000 selection intensity, adopt
With five plants of select trees wood methods, select tree is selected in Comprehensive Assessment.
2. method according to claim 1, it is characterised in that：Step 1）It is fine after selection at 10 points in the morning during collection branch
Bright positive day, after collection, cuts the blade of branch 1/2 ~ 5/6, and is tied with the encirclement of paper, film or cloth, to protect in transit
Hold branch moisture.
3. method according to claim 1, it is characterised in that：Step 2）Described in gather branch the same day sterilization, specifically
Operate and be：Branch blade is cut off, retains petiole 0.5 ~ 1.5cm of length, clean branch with detergent, and rinsed with clear water；Exist again
On superclean bench, by above-mentioned branch from terminal bud down, be cut to 3~4 sections, every section at least retains an axillary bud, and mercuric chloride is used respectively
8~30min of sterilization, then it is clean with sterile water wash.
4. method according to claim 1, it is characterised in that：Step 5）Light intensity during middle experienced seedling is 5000 ~ 8000Lx, temperature
Spend is 15 ~ 38 DEG C；Practice the seedling time for 2 ~ May or 9 ~ November.
5. method according to claim 1, it is characterised in that：Step 6）The time of middle transplanting be 2~June or 9~November,
When transplant time is 2~May, the period, rainfall is plentiful, and air humidity is big, with yellow soil and Light media volume ratio（6.0~
8.0）：（2.0~4.0）It is grafting matrix, with true yellow cubsoil as grafting matrix when other months transplant.
6. method according to claim 1, it is characterised in that：Step 6）Middle outplanting and management concrete operations are：Move
Cover film and shading screen after plant, the initial stage are 90~95% in sunshade rate, relative humidity more than 95%, and day temperature is 22~35 DEG C
Under conditions of cultivate 6~9 days, thereafter by sunshade rate, relative humidity be reduced to respectively 80~90% continuation cultivate 4~7 days, then 5
Relative humidity is gradually reduced in~7 days to removing film, then gradually reduces sunshade rate to removing, cultivate nursery stock under natural conditions
Up to more than 25cm.
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| CN109744142A (en) * | 2017-08-21 | 2019-05-14 | 中国林业科学研究院热带林业研究所 | A kind of santal tissue-culturing quick-propagation method for culturing seedlings |
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| CN112119911A (en) * | 2020-08-27 | 2020-12-25 | 广西壮族自治区林业科学研究院 | Method for effectively promoting subculture multiplication of albizia julibrissin |
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