CN105028485B - Regulation and control growth-promoting agent and the application process in crop field tea tree sprouting is expedited the emergence of in a kind of tea tree - Google Patents
Regulation and control growth-promoting agent and the application process in crop field tea tree sprouting is expedited the emergence of in a kind of tea tree Download PDFInfo
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- CN105028485B CN105028485B CN201510411184.5A CN201510411184A CN105028485B CN 105028485 B CN105028485 B CN 105028485B CN 201510411184 A CN201510411184 A CN 201510411184A CN 105028485 B CN105028485 B CN 105028485B
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Abstract
The present invention proposes a kind of preparation and application method of the interior regulation and control growth-promoting agent of tea tree.It prepares sward spirillum culture solution in tea tree, and spray the tea tree branch trimmed after diluting according to a certain ratio using the Biological characteristics of sward spirillum in tea tree with its fermentation.Bacterium in growth-promoting agent enters tea tree branch endosymbiosis and secretes metabolic active substance, the fast-growth for promoting tea tree newly to sprout.Treated, and tea tree newly sprouts that growth is vigorous, and blade is dark green, and the length and weight newly sprouted obviously increase.The technical method of the present invention is at low cost, easy to operate, green safe, effect is more, persistent.
Description
Technical field
It newly sprouts interior regulation and control growth-promoting agent the present invention relates to plant, especially a kind of interior sward spirillum of application promotes crop field
The method of tea tree sprouting lateral bud growth.
Background technology
Tea tree is the important industrial crops in China, and there is cultivation in each province in south China.By certain after tea picking
Technique can be fabricated to green tea, white tea, yellow tea, black tea and black tea, and tea branch can make stem tea.In addition to as drink, tealeaves is effective
Ingredient can be additionally used in medicine, food, chemical industry etc..Tea tree be to harvest young tender shoots leaf as the perennial industrial crops of main object,
Newborn green nourishing tender tip can be repeatedly picked from tea tree every year.It is different according to season of picking, usually have spring tea, summer tea and
Point of autumn tea.Repeatedly picking must expend a large amount of nutrition of tea tree.How to maintain the in great numbers of tree body and continue to expand regrowth,
Meet tea tree robust growth, is allowed to high-quality, high and stable yields are a vital tasks that tea place field management faces.In order to improve tea
Leaf-making quantity, other than daily management, mainly by the rational application of fertilizer to meet tea tree nutritional need.In addition, using gibberellin, 30
The plant hormone substances such as alkanol, compound sodium nitrophenolate, 6-benayl aminopurines, brassin lactones, 4- iodobenzenes ethoxyacetic acid (production increasing agent)
Promote tea shoot growth to improve tea yield.Though artificial synthesized plant hormone has the advantages that at low cost, growth-promoting functions are fast,
Their effects are single, effect not persistently, it is stringent using concentration requirement and with pedo relict risk, be unsatisfactory for green ecological environmental protection
It is required that the shortcomings that.In addition, artificial synthesized plant hormone stimulates the division and growth of plant cell mostly.If tea tree nutrition
It does not keep up with, it is repeated multiple times to be possible to influence the accumulation of nutritional ingredient in newborn tea cell using artificial synthesized plant hormone.
Sward spirillum is a kind of endogenetic bacteria that tea tree symbiosis specificity is strong in tea tree.It is colonized by wound in tea tree body
It is interior, regulate and control and promotes growth of tea plant.Document " Microbiological Characteristics of sward spirillum in two plants of tea trees " (Wang Ting etc., microbiology
Report 2014 the 4th phases, 2014.04.04, P424-432) screening of sward spirillum and Microbiological Characteristics in tea tree are described in detail,
But it is not directed to the application of sward spirillum in tea tree.
Invention content
The purpose of the present invention is to propose to regulate and control the preparation and application method of growth-promoting agent in a kind of tea tree.It is handled with the growth-promoting agent
Tea tree branch after cutting can effectively facilitate the growth of tea tree branch lateral bud, improve tea picking amount.
The invention is realized in this way.Its step is
1, regulate and control the preparation of growth-promoting agent in tea tree
Take sward spirillum strain in the tea tree of -80 DEG C of preservations, inoculation 5mL nutrient broth mediums (10g tryptones, 3g
Powdered beef, 5g NaCl, 10 μ g/mL spectinomycins, 5 μ g/mL ampicillins add water to 1000ml), 150 turns per minute are shaken
It is dynamic, 37 DEG C of overnight incubations.By the bacterium solution after activation with 1:100 ratios access fresh LB liquid medium (10g tryptones,
5g yeast extracts, 10g NaCl, 10 μ g/mL spectinomycins, 5 μ g/mL ampicillins add water to 1000ml, pH7.2-
7.4) amplification culture, condition of culture are in:200 turns per minute of shaking speed, temperature are 28-37 DEG C.Wait for that Bacteria Culture 6-8 is small
When, OD600Whole inoculums is collected when value is 1.0-1.2, be added after 1% glycerine deposit in 4 DEG C it is spare or directly make
With.
2, the preparation of working solution
In use, sward spirillum bacterium solution will be amplified in cultured tea tree by 0.5-2：It is work that 1 ratio clear water, which dilutes,
Make liquid.
Inoculum after above-mentioned dilution is to regulate and control growth-promoting agent in tea tree of the present invention.
The application of the growth-promoting agent：
1, tea tree branch is handled：Routinely tea bush trimmer method will be repaiied with electric slicer at the top of the tea tree of field production
Shearing is cut, and tea tree branch upper end is made to generate notch.
2, accelerating agent will be regulated and controled in growth of tea plant with sprayer to be sprayed at the top of tea tree.Its dosage is 50-100ml/ squares
Rice.
3, routinely field management method is managed after the completion of sprinkling.
The mechanism of the present invention is such, and sward spirillum is the endophyte of Theaceae plant in tea tree, only passes through wound
Into in tea tree body, cell exosymbiosis.After the tea tree shearing of crop field, sward spirillum can be cut in sprinkling by tea branch upper end in tea tree
Mouth enters tea tree branch and colonizes wherein.After colonizing, in tea tree sward spirillum and tea tree symbiosis and provide bacterial factor such as IAA,
NH4 +Isoreactivity substance promotes the growth of tea tree sprouting or lateral bud.Sward spirillum is natural tea tree endophyte in tea tree, is not infected
Other crops, disease-free effect usually only colonize in older branch or blade, without bacterium in newborn spray tender leaf,
Meet environmentally protective and food safety requirements.
The present invention has the advantages that uniqueness：
1, the Biological characteristics of sward spirillum in tea tree are taken full advantage of.Sward spirillum is that Camellia Plants are single-minded in tea tree
The strong endogenetic bacteria of property, it is nuisanceless with tea tree mutualistic symbiosis.It can effectively generate IAA, the NH for promoting growth and development of plants4 +、
Thermophilic siderophore isoreactivity substance.IAA can irritate tea tree cell growth, NH4 +Nitrogen source can be provided for tea tree, thermophilic siderophore can be tea tree
It adsorbs iron ion, improve tea tree disease resistance, with IAA, NH4 +Collaboration promotes growth of tea plant development together.Use sward in tea tree
Spirillum sprinkling tea tree branch can realize the efficient utilization of endogenetic bacteria metabolite, of low cost, easy to operate, safety and environmental protection.
2, overcome plant hormone have a single function, act on not persistently, cannot effectively facilitate sprouting subsequent growth development lack
Point.Sward spirillum colonizes after entering tea tree branch in tea branch in tea tree, sustainable to play a role, and is not required to repeatedly spray wine.It is not
Only promote the fast-growth newly sprouted, and is expected to improve the quality of tea tree.
Description of the drawings
Fig. 1 is the average length of newborn tea branch in 2-4 of the embodiment of the present invention,
Fig. 2 is the weight of 100 plants of newborn tea branches in 2-4 of the embodiment of the present invention,
Wherein control group is clear water processing.
Specific implementation mode
With reference to embodiment, the present invention is further described.
Embodiment 1:
Take sward spirillum strain in the tea tree of -80 DEG C of preservations, inoculation 5mL nutrient broth mediums (10g tryptones, 3g
Powdered beef, 5g NaCl, 10 μ g/mL spectinomycins, 5 μ g/mL ampicillins add water to 1000ml), 150 turns per minute are shaken
It is dynamic, 37 DEG C of overnight incubations.By the bacterium solution after activation with 1:100 ratios access fresh LB liquid medium (10g tryptones,
5g yeast extracts, 10g NaCl, 10 μ g/mL spectinomycins, 5 μ g/mL ampicillins add water to 1000ml, pH7.2-
7.4) amplification culture, condition of culture are in:200 turns per minute of shaking speed, temperature are 28-37 DEG C.Wait for that Bacteria Culture 6-8 is small
When, OD600Whole inoculums is collected when value is 1.0-1.2, after 1% glycerine is added, deposit in 4 DEG C it is spare or directly make
With.In use, sward spirillum bacterium solution will be amplified in cultured tea tree by 0.5-2：It is growth-promoting agent that 1 ratio clear water, which dilutes,.
Embodiment 2:By 1:Regulation and control growth-promoting agent liquid sprays Hubei Province tea 1 after 50 square metres of cuttings in 2 diluted 3000mL tea trees
Tea tree, the tea tree sprouting after 60 days grows fine, blade is dark green, and average length of newly sprouting increases 3.6cm than control group, and 100 is new
Branch weight ratio control group increases by 14.6 grams.(see Fig. 1 and 2).
Embodiment 3:By 1:Regulation and control growth-promoting agent liquid sprays Hubei Province tea 1 after 50 square metres of cuttings in 1 diluted 3000mL tea trees
Tea tree, the tea tree sprouting after 60 days grows fine, blade is dark green, and average length of newly sprouting increases 5.1cm than control group, and 100 is new
Branch weight ratio control group increases by 19.7 grams.(see Fig. 1 and 2).
Embodiment 4:By 2:Regulation and control growth-promoting agent liquid sprays Hubei Province tea 1 after 50 square metres of cuttings in 1 diluted 3000mL tea trees
Tea tree, the tea tree sprouting after 60 days grows fine, blade is dark green, and average length of newly sprouting increases 5cm, 100 shoots than control group
Weight ratio control group increases by 19 grams.(see Fig. 1 and 2).
Claims (1)
1. application of the regulation and control growth-promoting agent in crop field tea tree sprouting is expedited the emergence of in a kind of tea tree, it is characterised in that：Sward spiral shell in tea tree
Bacterium, which is entered by tea tree top notch in tea tree branch, to be colonized, and the interior sward spirillum after colonizing can promote tea tree side by interior adjusting
Bud is grown, and realizes that tea place weight-reducing and tea yield increase, this described is as follows using specific implementation step：
(1) regulate and control the preparation of growth-promoting agent in tea tree：Sward spirillum strain in the tea tree of -80 DEG C of preservations is taken, 5mL nutrient broths are inoculated with
Culture medium, 150 turns of shakes per minute, 37 DEG C of overnight incubations, by the bacterium solution after activation with 1:100 ratios access fresh LB liquid
Culture is amplified in culture medium, fermentation culture conditions are:200 turns per minute of shaking speed, temperature are 28-37 DEG C, pH7.2-
7.4, wait for bacterial fermentation culture 6-8 hours, OD600Whole inoculums is collected when value is 1.0, is stored after 1% glycerine is added
It is spare in 4 DEG C；Amplification culture solution is pressed into 0.5-2 when use：1 ratio is diluted with clear water, is to regulate and control growth-promoting in tea tree after mixing
Agent；
The nutrient broth medium is 10g tryptones, and 3g powdered beefs, 5g NaCl add water to 1000ml, and 10 μ g/mL are grand
Mycin, 5 μ g/mL ampicillins；
The LB liquid medium is 10g tryptones, and 5g yeast extracts, 10g NaCl add water to 1000ml, 10 μ g/mL
Spectinomycin, 5 μ g/mL ampicillins；
(2) in tea tree regulation and control growth-promoting agent use, routinely tea bush trimmer method, with electric slicer by the tea tree of field production
Top trimming cutting, makes tea tree branch upper end generate notch, and will regulate and control growth-promoting agent in tea tree with sprayer is sprayed at the top of tea tree,
Make in tea tree sward spirillum enter in tea tree branch to colonize, dosage is 50-100ml/ square metres, routinely field after the completion of sprinkling
Between management method be managed.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7084331B2 (en) * | 2002-01-15 | 2006-08-01 | Society for Techno-Innovation of Agriculture Forestry and Fisheries | Rice containing endophytic bacteria and method of producing it |
| CN104585241A (en) * | 2015-01-13 | 2015-05-06 | 湖北大学 | Preparing and applying method of cutting propagation growth promoting agent of tea tree endogenous herbaspirillum seropedicae |
| CN104704109A (en) * | 2012-09-19 | 2015-06-10 | 生物探索（新西兰）有限公司 | Methods of screening for microorganisms that impart beneficial properties to plants |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7084331B2 (en) * | 2002-01-15 | 2006-08-01 | Society for Techno-Innovation of Agriculture Forestry and Fisheries | Rice containing endophytic bacteria and method of producing it |
| CN104704109A (en) * | 2012-09-19 | 2015-06-10 | 生物探索（新西兰）有限公司 | Methods of screening for microorganisms that impart beneficial properties to plants |
| CN104585241A (en) * | 2015-01-13 | 2015-05-06 | 湖北大学 | Preparing and applying method of cutting propagation growth promoting agent of tea tree endogenous herbaspirillum seropedicae |
Non-Patent Citations (1)
| Title |
|---|
| 两株茶树内生草螺菌的微生物学特性;王婷 等;《微生物学报》;20140404;第54卷(第4期);第424-432页 * |
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