CN105009900A - Method for manufacturing moss sculpture - Google Patents

Method for manufacturing moss sculpture Download PDF

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Publication number
CN105009900A
CN105009900A CN201510431899.7A CN201510431899A CN105009900A CN 105009900 A CN105009900 A CN 105009900A CN 201510431899 A CN201510431899 A CN 201510431899A CN 105009900 A CN105009900 A CN 105009900A
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China
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moss
green carving
liver moss
breeding
stoste
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CN201510431899.7A
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CN105009900B (en
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吴军
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H11/00Bryophytes, e.g. mosses, liverworts

Abstract

The invention provides a method for manufacturing a moss sculpture, wherein the method relates to the field of an environment-friendly handicraft. The method is an application of open moss tissue culture technology. The method comprises the steps of performing preliminary treatment on a moss raw material; mixing the moss raw material with a culture medium for preparing a moss stock solution; transferring the moss stock solution onto a sculpture frame; controlling a moss development direction through controlling the film forming state of the moss stock solution on the surface of the sculpture frame, thereby culturing the moss; and finally preparing various moss sculpture shapes. The moss sculpture which is manufactured by the method of the invention has advantages of low production cost, vivid shape, convenient culturing, and high suitability for batch production in a factory and personal shape manufacture.

Description

The preparation method of a kind of moss green carving
Technical field
The present invention relates to friendly process product field, in particular to the preparation method of a kind of moss green carving.
Background technology
The breeding method of current liver moss moulding mainly adopts the mode of field planting glue to cultivate.First, by clean for the mould such as wooden model surface clean being used for cultivating liver moss moulding, then, by field planting glue uniform application at die surface, finally, fresh liver moss is transplanted to the die surface scribbling field planting glue and cultivates, adopt the liver moss moulding cultivated in this way and there is certain aesthetic feeling.But by liver moss being transplanted to the method that the die surface scribbling field planting glue carries out cultivating, migration process is very loaded down with trivial details, is not easy to large-scale production, the liver moss of therefore its liver moss moulding covers few.Simultaneously, wooden model adopt field planting glue for the limited efficiency of liver moss adhesive water and nutriment, the liver moss moulding needs maintenance very frequently causing this mode to be cultivated, and artificial graft's seedling liver moss can cause its gametophyte and sporophyte natural beauty greatly to reduce.
Summary of the invention
In view of this, the object of the embodiment of the present invention is the breeding method providing a kind of green carving, to improve above-mentioned problem.
A preparation method for moss green carving, comprises the following steps:
(1) the liver moss raw material of collection is carried out preliminary treatment; (2) liver moss stoste is mixed by the medium needed for moss growth and by the liver moss raw material after step (1) processes; (3) make green carving skeleton and described liver moss stoste be transferred to the green carving skeleton through sterilizing; (4) the green carving skeleton being coated with described liver moss stoste is positioned in breeding device; (5) control the growth direction of liver moss according to the film-forming state of the liver moss stoste being covered in green carving skeleton surface, carry out moss cultivation.
Further, the described preliminary treatment in step (1) comprises following operation: (1.1) are first to the liver moss raw material cleaning decontamination gathered; (1.2) carrying out sterilization treatment to removing the liver moss raw material after impurity, then liver moss raw material being anhydrated process, making liver moss raw material be moisture state.
Further, the described medium in step (2) comprises following composition: the nutritive element mother liquor needed for (2.1) moss growth, plant growth regulator mother liquor, insecticide, anti-browning agent, anti-pollutant, carbohydrate and yeast juice; (2.2) single type artificial endosperm or mixed type artificial endosperm: single type artificial endosperm: by film forming agent soaked or stir be made; Mixed type artificial endosperm: the soaked or stirring by film forming agent, then mixes starch component and film forming agent.
Further, described preparation liver moss stoste in step (2) comprises multi-step mixing facture or synchronously stirs facture: multi-step mixing facture: be mixed in container by each component in step (2.1), add water in container and make the temperature of liquid be 20 DEG C ~ 45 DEG C, then put into agitator together with the liver moss raw material through preliminary treatment and stir 1-2 minute; Again the single type artificial endosperm of making or mixed type artificial endosperm are put into agitator, then stir 1-2 minute; In agitator, add pH value regulator is neutral with adjust ph or faintly acid, obtains liver moss stoste; Synchronous stirring facture: each component in step (2.1) is mixed in container, add water in container and make the temperature of liquid be 20 DEG C ~ 45 DEG C, then with through the liver moss raw material of preliminary treatment, single type artificial endosperm or mixed type artificial endosperm put into agitator, stir 0.5-2 minute; In agitator, add pH value regulator is again neutral with adjust ph or faintly acid, obtains liver moss stoste.
Further, the making green carving skeleton in step (3) the green carving skeleton be transferred to through sterilizing comprises following operation by described liver moss stoste:
(3.1) make green carving skeleton and by the first soaked cleaning of green carving skeleton, then put into the sterilized solution timing sterilization of constant volume or by illumination sterilizing, described green carving skeleton comprises metal net layer, surface layer and suture; Described surface layer is laid on described metal net layer, and described metal net layer and described surface layer are fixed by described suture;
(3.2) use the green carving skeleton after clear water drip washing sterilization treatment again, make green carving skeleton keep moisture state afterwards;
(3.3) liver moss stoste is transferred to the green carving skeleton in moisture state, make the liver moss stoste being covered in green carving skeleton surface reach not landing, the method for described transfer comprises any one or more in spraying, brushing, dip-coating.
Further, the described breeding device in step (4) comprises as one of lower device:
The reflective breeding device of buckle-type: be made up of breeding box, mesh-supported frame, reflex reflector and the breeding cover that made by light transmissive material.The cover top of breeding cover or top, side are provided with air-vent, fill water in described breeding box, and mesh-supported erection is placed in breeding box and away from the water surface, green carving skeleton is positioned on mesh-supported frame, and the side seam being located at breeding box buckled by breeding cover; Reflex reflector is made up of reflective membrane and reflector, and reflective membrane is layed in breeding box inwall and all sides, and reflector is located at outside breeding box side;
The reflective breeding device of split: be made up of breeding box, bracing frame, reflex reflector and the breeding cover that made by light transmissive material.The cover top of breeding cover or top, side are provided with air-vent, fill water in breeding box, and bracing frame is arranged in breeding box and away from the water surface, green carving skeleton is positioned on bracing frame, and breeding cover is buckled and is located in breeding box and stretches under the water surface.Reflex reflector is made up of reflective membrane and reflector, and reflective membrane is layed on bracing frame or is layed in breeding box inner circumferential side, and reflector is located in breeding box or outside breeding box.
Further, film-forming state described in step (5) is main control by the kind of film forming agent, component and starch component, described film forming agent is the compound of water-loss reducer or water-loss reducer and essence of loosening the soil, described medium contains starch component, and the growth direction of described liver moss is protonema;
Film forming judges feature: liver moss stoste covers green carving skeleton surface completely, and has reflective;
Principal character in cultivating process: the viridescent protonema in green carving skeleton surface produces;
The cultivating process of protonema comprises protonema induction period and protonema seeding stage;
Protonema induction period: time 20-35 days, control temperature 18-25 DEG C except fine day, intensity of illumination is 0-8000LX, humidity 75-97%; Fine day controls the temperature at green carving skeleton place within 30 DEG C, and within one week, can carry out a fine day illumination in interval, intensity of illumination controls at below 12000LX, and continuous sunny need do process of sheltering from heat or light, cloudy without the need to sheltering from heat or light;
Protonema grows and comprises one of following two kinds of breeding methods seedling stage:
First cultivating method: green carving surface presentation is thallus, protonema, cormus, callus, four kinds of states, cultivates without heating outside transfer chamber, temperature 5-25 DEG C, illumination control 0-25000LX, humidity 60-95%;
Second cultivating method: protonema is divided into thallus state, cultivates without heating outside transfer chamber, illumination control 0-4500LX, temperature 5-25 DEG C, humidity 60-90%.
Further, film-forming state in step (5) is main control by the kind of film forming agent and component, and described film forming agent is essence of loosening the soil, the not starch-containing composition of described medium, breeding method comprises the first cultivating method and the second cultivating method, and it is with the numerous bud of bud that described first cultivating method grows direction; It is protonema that described second cultivating method grows direction;
Film forming judges feature: liver moss stoste covers green carving skeleton surface completely;
First cultivating method comprises with bud numerous bud inducement phase with bud numerous bud seeding stage:
Principal character in first cultivating method cultivating process: green carving skeleton surface has young moss seedlings to produce;
Second cultivating method comprises protonema induction period, protonema seeding stage;
Principal character in second cultivating method cultivating process: green carving skeleton surface has a large amount of protonema to produce, and produces with a small amount of young moss seedlings;
With bud numerous bud inducement phase: time 20-30 days, temperature 5-28 DEG C, illumination below control 3500LX, humidity 60-90%;
With bud numerous bud seeding stage: temperature 5-28 DEG C, illumination below control 3500LX, humidity 60-90%;
Protonema induction period: time 25-45 days, temperature 18-25 DEG C, illumination control 0-8000LX, humidity 60-90%;
The protonema seeding stage: temperature 5-28 DEG C, illumination below control 3500LX, humidity 60-90%;
Further, the film-forming state in step (5) is main control by the kind of film forming agent, component and starch component, and described growth direction is with the numerous bud of bud; First cultivating method film forming agent essence of loosening the soil, starch component is potato composition; The compound of the second cultivating method film forming agent sodium alginate or sodium alginate and essence of loosening the soil, starch component is tapioca starch.
Film forming judges feature: liver moss stoste covers green carving skeleton surface completely;
, in this breeding method, there are one of following two kinds of features in principal character in cultivating process:
First cultivating method feature: liver moss stoste was transferred to green carving skeleton surface after 1-3 days, be exposed to illumination and assign comprehensively dry to carry out sterilizing, green carving skeleton surface has young moss seedlings to produce;
Second cultivating method feature: without the need to being exposed to sterilizing under illumination after liver moss stoste is transferred to green carving skeleton surface, green carving skeleton surface has young moss seedlings to produce;
Comprise with bud numerous bud inducement phase with bud numerous bud seeding stage with bud numerous bud cultivating process;
One of following two kinds of breeding methods are comprised with bud numerous bud inducement phase:
First cultivating method: liver moss stoste is transferred to green carving skeleton surface 1-3 days, is then exposed to illumination and assigns comprehensively dry to carry out sterilizing, and then soak or spray water and put shady place, cultivate 20-30 days continuously;
Second cultivating method: after liver moss stoste is transferred to green carving skeleton surface, cultivates 20-30 days continuously;
The breeding condition of the first cultivating method and the second cultivating method comprises temperature 5-28 DEG C, illumination below control 3500LX, humidity 60-90%;
With bud numerous bud seeding stage: temperature 5-28 DEG C, illumination below control 3500LX, humidity 60-90%, penetrates in 1 hour, to help growth with 15000-25000LX illumination 1-2 time weekly.
Further, film-forming state described in step (5) is main control by the kind of film forming agent, component and starch component, described film forming agent is the compound of water-loss reducer or water-loss reducer and essence of loosening the soil, and described medium contains starch component, and described growth direction is callus;
Film forming judges feature: liver moss stoste covers green carving skeleton surface completely and has reflective; Principal character in cultivating process: after induction period, green carving skeleton surface has callus and bud to produce;
Callus cultivating process comprises the callus induction phase, callus turns protonema and the protonema seeding stage; The callus induction phase: time 20-30 days, temperature 20-30 DEG C, illumination below control 2500LX, humidity 70-90%;
Callus turns the protonema phase: time 25-45 days, temperature 15-25 DEG C, illumination control 3500-15000LX, humidity 60-90%; The protonema seeding stage: temperature 5-28 DEG C, illumination below control 3500LX, humidity 60-90%.
Moss cultivation is carried out in the growth direction that moss green carving preparation method provided by the invention controls liver moss by the film-forming state of the liver moss stoste controlling green carving skeleton surface, thus makes moss green carving.Liver moss stoste directly affects the growth direction of liver moss at the film-forming state on green carving skeleton surface, makes the cultivation of liver moss have better controllability.
The moss green carving that this method makes has feature clean, attractive in appearance, and moulding is lively, due to breeding method can be selected neatly, therefore be applicable to and allly view and admire liver moss, and effectively can reduce in moss green carving manufacturing process the frequency and the difficulty of maintenance work when cultivating liver moss, also improve disease-resistant, the worm ability of cultivating liver moss in addition.This preparation method is simple, convenient operation, greatly can reduce it and produce liter originally, be very suitable for large-scale production.
For making above-mentioned purpose of the present invention, feature and advantage become apparent, preferred embodiment cited below particularly, and coordinate appended accompanying drawing, be described in detail below.
Accompanying drawing explanation
In order to be illustrated more clearly in the technical scheme of new embodiment of the present invention, be briefly described to the accompanying drawing used required in embodiment below, be to be understood that, the following drawings illustrate only some embodiment of the present invention, therefore the restriction to scope should be counted as, for those of ordinary skill in the art, under the prerequisite not paying creative work, other relevant accompanying drawings can also be obtained according to these accompanying drawings.
The flow chart of the preparation method of the moss green carving that Fig. 1 provides for the embodiment of the present invention one;
The structural representation of the green carving skeleton of the use in the preparation method of the moss green carving that Fig. 2 provides for Fig. 1.
Description of reference numerals
Stratum reticulare 101; Surface layer 102; Suture 103.
Embodiment
Below in conjunction with accompanying drawing in the embodiment of the present invention, be clearly and completely described the technical scheme in the embodiment of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.The assembly of the embodiment of the present invention describing and illustrate in usual accompanying drawing herein can be arranged with various different configuration and design.Therefore, below to the detailed description of the embodiments of the invention provided in the accompanying drawings and the claimed scope of the present invention of not intended to be limiting, but selected embodiment of the present invention is only represented.Based on embodiments of the invention, the every other embodiment that those skilled in the art obtain under the prerequisite not making creative work, all belongs to the scope of protection of the invention.
Embodiment one
Consult Fig. 1, present embodiments provide the preparation method of a kind of moss green carving, this preparation method comprises the following steps:
(1) the liver moss raw material of collection is carried out preliminary treatment, liver moss raw material can be the gametophyte, the dry end of broken section of liver moss etc. of various liver moss.(2) liver moss stoste is mixed by the medium needed for moss growth and by the liver moss raw material after step (1) processes.(3) make green carving skeleton and described liver moss stoste be transferred to the green carving skeleton through sterilizing.(4) the green carving skeleton being coated with described liver moss stoste is positioned in breeding device.(5) moss cultivation is carried out in the growth direction controlling liver moss according to the film-forming state of liver moss stoste being covered in green carving skeleton surface.
Wherein, the film-forming state of liver moss stoste refers to after liver moss stoste is covered in the surface of green carving skeleton, the attachment state of liver moss stoste, such as, in example by after water-loss reducer is soaked again high speed 25000r/m stir, water-loss reducer is made to become thicker (water-loss reducer consumption is lower slightly) or dense thick (water-loss reducer consumption is higher) from coarse gluey, gel strength is high, such as, during water board water-loss reducer is introduced: owing to introducing the inorganic raw material of rigidity in product, in centrifuge 3000r/m after centrifugal 30 minutes water retention be greater than 93%, can holding capacity 250g ~ 500g/cm2; After mix and blend become liver moss stoste to compare thickness, current be transferred to green carving skeleton surface after, the pressure ratio that liver moss raw material is subject to liquid is comparatively large, and the pressure that moss growth is emerged is also large, increases starch based composition and can increase its pressure further; Such as with in water adhesion agent loosen the soil essence soaked after, synchronously again stir the liver moss stoste good fluidity that formed or when having certain foam, current be transferred to green carving skeleton surface after, when can adhere to shaping or form certain alveolate texture, the pressure that liver moss raw material is subject to liquid is smaller, and the pressure that moss growth is emerged is also little.The pressure that the process of growth and liver moss of liver moss are subject to self is relevant, liver moss stoste then can play crucial effect to the pressure of liver moss at the film-forming state on green carving skeleton surface, so be covered in the film-forming state of the liver moss stoste on green carving skeleton surface and coordinate environmental Kuznets Curves directly can have influence on the growth direction of liver moss.The existence of the film-forming state of liver moss stoste and the kind of film forming agent and component, starch material whether, the denseness of medium, the broken segment length of liver moss, the factor such as transfer operation be relevant.Such as, film-forming state is by water hydraulic control system: the content controlling water in medium; Film-forming state is controlled by the amount of liver moss raw material: the volume and/or the quality control that control liver moss raw material; Film-forming state is controlled by the broken segment length of liver moss raw material: the length controlling broken section according to the Different growing environment of liver moss; Film-forming state is controlled by the concrete grammar of transfer operation.Such as when liver moss stoste is rarer, broken section long or formed be uneven when green carving surface can not form reflective, the pressure that part liver moss is subject to is little, in the winter time when the induction of protonema method or the induction of callus method, still there will be part liver moss seedling to occur, then can not form Cultivated objective uniformity.
Preferably, liver moss stoste covers green carving skeleton surface completely, and liver moss stoste can not landing.Film forming agent comprises three kinds: the compound of gel class, water adhesion agent class, gel-like and water adhesion agent.The film forming agent of gel class is as large in the one-tenth film pressure of water-loss reducer etc., thus comparatively large to the growth pressure of liver moss, further, can add starch based to increase to film pressure in gel class film forming agent.The one-tenth film pressure of film forming agent as loosen the soil essence or konjac glucomannan etc. of water adhesion agent class is little, therefore also relatively little to the growth pressure of liver moss.
Because part bacterium can consume the various nutrient components needed for moss growth in the growth and development process of liver moss, also discharge the material poisonous to liver moss simultaneously, thus affect the normal growth of liver moss.Therefore, can carry out to liver moss raw material the bacterium, foreign material etc. that preliminary treatment carries to reduce it.First, the liver moss raw material gathered is cleaned, remove the impurity in liver moss raw material, such as wood chip, sandstone, rotted leaf etc.Secondly, the liver moss raw material after decontamination is carried out sterilization treatment, such as, carries out cleaning, utilizing the washing powder with bactericidal action to clean with clear water.Again, to anhydrate process to liver moss raw material, make liver moss raw material keep moisture state, object is beneficial to control liver moss stoste water content, is beneficial to liver moss simultaneously and absorbs nutrient component.Moisture state and liquid film are formed at the state on the whole surface of liver moss raw material, and such as, broken section of liver moss is covered by liquid film.Preferably, sterilization treatment can also be carried out to green carving skeleton, avoid being attached to bacterium on green carving skeleton to the impact of moss growth.Such as, high-temperature sterilization, ultraviolet sterilization etc. mode can be adopted to carry out sterilization treatment to green carving skeleton.In addition, cleaning liver moss raw material, sterilization treatment time should note avoiding causing damage to it, and affect the normal growth of liver moss raw material.
After completing the preliminary treatment of liver moss raw material, then make liver moss stoste, this liver moss stoste comprises the medium needed for moss growth and the liver moss raw material through preliminary treatment.Medium is that the growth of liver moss provides various nutritive element, growing environment and other growth promoting effects and suppresses the composition of damage by disease and insect.In the present embodiment, medium comprises following component: the nutritive element mother liquor needed for moss growth, plant growth regulator mother liquor, insecticide, anti-browning agent, antipollution, carbohydrate, yeast juice and artificial endosperm, wherein artificial endosperm comprises two kinds, is respectively single type artificial endosperm and mixed type artificial endosperm.Wherein, single type artificial endosperm is made by film forming agent is soaked or stirring; Mixed type artificial endosperm by film forming agent is soaked or stirring, and then forms with starch component mixing manufacture.
Then can carry out the making of liver moss stoste after medium completes, preparation liver moss stoste comprises multi-step mixing facture and synchronous stirring facture two kinds of methods.
Wherein, multi-step mixing facture: first, be mixed in a container to the nutritive element mother liquor needed for above-mentioned moss growth, plant growth regulator mother liquor, insecticide, anti-browning agent, antipollution, carbohydrate, yeast juice, and add water in this container and make the temperature of liquid be 20 DEG C ~ 45 DEG C.Secondly, put into agitator with the liver moss raw material through preliminary treatment and stir 1-2 minute, be preferably 1 minute, be beneficial to the broken segment length of control and reach requirement and save time.Again, single type artificial endosperm or mixed type artificial endosperm are added in agitator and stir 1 ~ 2 minute.Finally, in agitator, add pH value regulator is neutral with adjust ph or faintly acid, and obtain liver moss stoste, pH value regulator can adopt sodium hydrate aqueous solution or hydrochloride aqueous solution.
Synchronous stirring facture: first, be mixed in a container to the nutritive element mother liquor needed for above-mentioned moss growth, plant growth regulator mother liquor, insecticide, anti-browning agent, antipollution, carbohydrate, yeast juice, and add water in this container and make the temperature of liquid be 20 DEG C ~ 45 DEG C.Secondly, put into agitator with through the liver moss raw material of preliminary treatment, single type artificial endosperm or mixed type artificial endosperm, stir 0.5-2 minute, be preferably 0.5 minute, be beneficial to and control broken segment length and reach requirement and save time; In agitator, add pH value regulator is again neutral with adjust ph or faintly acid, obtains liver moss stoste.PH value regulator can adopt sodium hydrate aqueous solution or hydrochloride aqueous solution, and the pH value of liver moss stoste is preferably 6.5≤PH≤7, and such as 6.5,6.6,6.8 or 7.
After liver moss stoste completes, then can transfer them to green carving skeleton surface, detailed process is as follows: step 1: first, by the first soaked cleaning of green carving skeleton, to remove the spot etc. on green carving skeleton surface.Then, sterilization treatment is carried out to green carving skeleton, avoid the growth of green carving skeleton Carried bacteria to liver moss to cause interference.Particularly, the sterilized solution of constant volume can be used as first used washing powder, then carry out timing, quantitatively sterilizing with such as bromogeramine liquid or other sterilized pharmaceutics, or by illumination sterilizing.Step 2: by the green carving skeleton clear water drip washing processed through step 1, makes green carving skeleton keep micro-wet, such as, can adopt the mode of drying, remove the excessive moisture on green carving skeleton surface.Due to, green carving skeleton surface is in moisture state, thus is conducive to attachment and the distribution of liver moss stoste; When adopting illumination sterilizing to green carving skeleton, green carving skeleton can be made directly to keep dry state.Step 3: liver moss stoste is transferred to the green carving skeleton in moistening or drying regime, makes the liver moss stoste not landing being covered in green carving skeleton surface.When the liver moss stoste on green carving skeleton surface is too much, then easily cause the density of liver moss raw material excessive, thus cause competition in moss growth process to affect its normal growth greatly, and the liver moss stoste of landing may pollute breeding device, and affect the growing environment of liver moss, therefore, preferably, the liver moss stoste being transferred to green carving skeleton surface is not reunited.The method of transfer comprises spraying or brushes or dip-coating, is not specifically limited in the present embodiment to concrete transfer method.
Green carving skeleton is the substrate of moss growth, and the shape of green carving skeleton limits the shape of final green carving skeleton.According to actual use scenes needs, the shape of green carving skeleton can have multiple, adopts method for folding that green carving carcasing is become all kinds of shape, such as, and various animal shape or various potted landscape profiles etc.The material of green carving skeleton can adopt wire netting, is preferably aluminium alloy net, and aluminium alloy net quality softness is convenient to be shaped and have good resistance to corrosion, light quality, is beneficial to the movement of later stage moss green carving.Green carving skeleton can also adopt fiber-like material, and be preferably scouring pad, its surface is relatively coarse, and it has a lot of micropore, under the prerequisite guaranteeing gas permeability, can also improve the attachment stability of liver moss stoste.Certain green carving skeleton can be used for also can be used for having earth culture to educate moss green carving without soil, also can be used for the green carving of Tom Thumb ornamental plants.
After liver moss stoste is transferred to green carving skeleton, then can green carving skeleton be positioned in breeding device, carries out the cultivation of liver moss.Present embodiments provide the reflective and reflective two kinds of breeding devices of split of buckle-type, can select two kinds of breeding devices or its part composition according to the demand of reality, breeding device is not limited to two kinds that the present embodiment provides.
Wherein, the reflective breeding device of buckle-type is made up of breeding box, mesh-supported frame and the breeding cover that made by light transmissive material.The cover top of breeding cover or top, side are provided with air-vent, and fill water in breeding box, preferably, breeding box inner drying fills water season, and wet weather is not filled with water in season.The end in breeding box, sidewall are preferably white or directly for having reflective membrane function, and mesh-supported erection to be placed in described breeding box and away from the water surface.Green carving skeleton is positioned on described mesh-supported frame, and the side seam being located at described breeding box buckled by breeding cover.
Preferably, seedling-cultivating tray and reflector can also be set up to the reflective breeding device of buckle-type, the reflective breeding device of buckle-type is positioned in seedling-cultivating tray, and reflector is arranged in seedling-cultivating tray or vicinity is arranged at outside breeding box, and is bent to the reflective breeding device of the contiguous buckle-type of arc-shaped.The reflecting angle of reflector is adjustable, and preferably, the reflecting angle of reflector controls to be 45 ~ 90 DEG C.For increasing the reflecting effect of reflector, at reflector, reflective membrane can be set towards the side of buckle-type reflective breeding device.Further, also can in breeding box the end and inwall lay reflex film, improving multi-angle reflect effect, when filling water in breeding box, also reflective membrane can be layed on bracing frame.Trying to get to the heart of a matter of seedling-cultivating tray can arrange through hole as the case may be, in order to discharge the water put aside in seedling-cultivating tray.
The reflective breeding device of split is made up of breeding box, bracing frame and the breeding cover that made by light transmissive material.The cover top of breeding cover or top, side are provided with air-vent, fill water in breeding box, and bracing frame to be arranged in breeding box and away from the water surface.Green carving skeleton is positioned on institute's mesh-supported frame, and breeding cover is buckled and is located in breeding box and stretches under the water surface.
Preferably, can also set up reflector to the reflective breeding device of split, reflector is arranged in breeding box or vicinity is arranged at outside breeding box, and is bent to the contiguous breeding cover of arc-shaped.The reflecting angle of reflector is adjustable, and preferably, the reflecting angle of reflector controls to be 45 ~ 90 DEG C.For increasing the reflecting effect of reflector, at reflector, reflective membrane can be set towards the side of buckle-type reflective breeding device.Further, also on breeding box bracing frame and breeding box inwall, all sides lay reflex film, multi-angle reflect effect can be improved.
Certain buckle-type and split reflective breeding device both can be used for moss green carving and had cultivated, and also can be used for its class plant cultivation.
Because green carving skeleton is away from the water surface, when the water in breeding box evaporates, the water of evaporation can carry out moisturizing to liver moss stoste, promotes the growth of liver moss raw material.In addition, evaporation of water can also play the effect regulating temperature in breeding device.Preferably, winter can also set up filter with in buckle-type reflective breeding device and the reflective breeding device of split, such as scouring pad, is layed in scouring pad on mesh-supported frame or mesh-supported frame, and the green carving skeleton being coated with liver moss stoste is positioned on scouring pad.When the evaporated quantity of water in breeding box is comparatively large, then can utilize scouring pad to filter, prevent that vapour-liquid temperature is too high causes the depauperation of liver moss raw material or the landing of liver moss raw material but the growth of liver moss is uneven.Preferably, be positioned in simple greenhouse by breeding device, such as, plastic tunnel, so that the environmental temperature controlling in breeding device.When the temperature of external environment is lower, in such as winter, heater can be set in breeding device, to promote the growth of liver moss raw material; When the temperature of external environment is higher, such as summer, then does not need in breeding device to arrange heater, and the reflective breeding device of split can be adopted to reach cooling-down effect to strengthen reservoir storage.When the humidity of external environment is higher, in overcast and rainy more June in such as summer, breeding device can reduce and to be filled with water or anhydrous, to control the moisture that liver moss reaches suitable growth.Strong owing to placing three daylightings of breeding device under simple greenhouse or tree shade, one side daylighting is weak, therefore also can adopt split or the reflective breeding device of buckle-type, in simple greenhouse, the reflective breeding device of buckle-type is because of without the need to considering rain proofness, therefore breeding is put and be can be top drilling, atresia at the bottom of seedling-cultivating tray available disk or porose seedling-cultivating tray; Need to consider rain proofness under outdoor tree shade, therefore breeding flat-top or dome or other type shape overhead guard change side roof part perforate into, rainwater can be naturally dirty and do not damage the green carving skeleton of breeding, seedling-cultivating tray adopts bottom to have sieve aperture dish, reaches the effect of buckle-type reflective breeding device rainproof and anti-dish internal water accumulation with this.
Film forming agent described in the present invention is essence of loosening the soil, the not starch-containing composition of described medium, and breeding method comprises the first cultivating method and the second cultivating method, and it is with the numerous bud of bud that described first cultivating method grows direction; It is protonema that described second cultivating method grows direction; Adopt split or the reflective breeding device of buckle-type again, because of easy and simple to handle and not easily endophytic bacteria and insect pest; In addition, can reach easy maintenance, multi-angle growth result, therefore be the green carving actual production best approach by split or buckle-type reflective breeding device without the need to exchanging light angle, therefore request is preferred breeding method.In the present invention, buckle-type is reflective with in the reflective two kinds of breeding devices of split, in breeding box, the end or side are preferably white or direct for having reflective membrane function, then in breeding box, the end or side are without the need to repaving reflective membrane, and reflective membrane also can substitute with the reflective mirror with reflective membrane function.The available konjac glucomannan without heating of water adhesion agent in the present invention, xanthans, carragheen or its built agent or other play similar action film forming agent as slightly poor the substituting of effect.Mix in the present invention in artificial endosperm and can be substituted as effect is slightly poor by similar action film forming agent other or starchy material; Water-loss reducer can with having the water-loss reducer long-lasting and stored nutrient of dilation repeatedly, and can be directly water-soluble and better replacement done by gel without the need to the tool rigid stirred.
Optimization Steps of the present invention and scheme order as follows: the liver moss raw material of collection carries out in preliminary treatment by step 1, liver moss raw material also can be put 4 DEG C of refrigerating chambers and makes low temperature treatment, liver moss raw material also can adopt peat growing medium breeding or the breeding of river sand nutrient solution in shade place, thus obtain the better gametophyte of activity, when sterilization treatment adds bactericidal agent in the medium, only can make simple and easy sterilization treatment with washing powder, without the need to the sterilizing again of the sterile medical such as bromogeramine, play saving working hour water-saving effect.Only not can be used as liver moss fluid sowing containing carbohydrate containing medium or medium with water adhesion agent in the present invention and can make the poor moss green carving of effect.In the present invention, medium be only that basic expansion is numerous containing carbohydrate and large moderate-element, can make the slightly poor green carving of effect.Add suitable for plant growth regulator and can expand numerous yarn number, the liver moss such as had only can expand numerous 16 with white sugar, and adds 2, and it is 18.5 that 4-D expands numerous yarn number, and concentration can at 0.1-1mg/L; Adding cytokinin such as 6-BA concentration can at 0.25-8mg/L; A-Nafusaku does growth hormone and hot-dry wind tolerance effect, and 3-butyric acid potassium makes short rooting effect; Add starch based as depot fertilizer, buffering, water suction, loose permeation functions can be played without filtration potato grain, juice; Angel Yeast can sharp moss growth and starch based composition effect of becoming thoroughly decomposed; Ascorbic acid plays BPH resistant rice variety effect; Active carbon or diatomite play antipollution, slow releasing function; First just reaching bright shield plays bactericide effect; Elder generation is just reaching sharp victory or chitosan plays insecticide effect, and clean water quality without insecticide or can change into spray insecticide to the ill in cultivating process; Diethyl aminoethyl hexanoate or multiple nitre have confusingly been received and have been assisted the growth of differentiation profit to improve resistance effect.In the present invention preferably by the illumination controled environment, temperature, humidity etc. because usually cultivating liver moss.The present invention be also applicable to wet in constant indoor temperature, constant humidity, permanent light or alternating temperature, change, to become under optical condition and individual layer or multi-layer rack carry out tissue cultures liver moss and makes moss green carving, in the environmental condition that can also provide from the present invention according to different liver moss kind equally or medium again suboptimization to reach best moss growth condition.Breeding method of the present invention is also applicable to the green carving skeleton of other applicable moss growth.Skeleton of the present invention and breeding device and breeding method are equally applicable to DIY client and cultivate green carving voluntarily.Lower slightly with the numerous bud of bud green carving liver moss density in breeding method of the present invention, the time is short, produces easily; The green carving liver moss density of protonema induction is high, and step is more, and environmental Kuznets Curves requires higher, and the time is long, and production difficulty is higher.
Embodiment two
The present embodiment provides preparation method the method for a kind of moss green carving to comprise the following steps:
The first step, carries out preliminary treatment by the liver moss raw material of collection: first, gathers moss and uprightly to grow thickly the gametophyte of the bent tail moss of many capsules doing to represent, then with water cleaning to remove the impurity such as earth, rubble, withered grass leaf, then clean with washing powder and water.Secondly, utilize bromogeramine reagent to the gametophyte sterilization treatment of the bent tail moss of the many capsules after cleaning, rinsed well by bromogeramine afterwards with water, finally the water in liver moss raw material being extracted the gametophyte making the bent tail moss of many capsules is moisture state.
Second step, mixes liver moss stoste by the medium needed for moss growth and by the liver moss raw material after step (1) processes:
The first medium comprises nutritive element mother liquor, plant growth regulator mother liquor, insecticide, BPH resistant rice variety and anti-pollutant needed for moss growth, carbohydrate, yeast juice and mixed type artificial endosperm.Particularly, the nutritive element mother liquor needed for moss growth: prepare with KNOP, potassium nitrate 250mg/L, potassium dihydrogen phosphate 250mg/L, nitrate of lime 1000mg/L, magnesium sulfate 250mg/L, zinc sulphate 3mg/L.Plant growth regulator mother liquor is prepared with following ratio: 6-BA1 ~ 8mg/L 0.1mol NaOH warm water hydrotropy solution, a-Nafusaku 0.5-2mg/L warm water hydrotropy solution, 3-butyric acid potassium 0.25-1mg/L warm water hydrotropy solution, DA-6 diethyl aminoethyl hexanoate 1-8mg/L warm water hydrotropy solution.Insecticide is first just reaching sharp victory 1:10000 (pesticide-seeds ratio), and carbohydrate is as white sugar 30g/L, and Angel Yeast 0.32g/L, ascorbic acid 2mg/L, active carbon 0.7g/L, in order to play the effect of BPH resistant rice variety, antipollution, sustained release agent in the process of growth of liver moss.Mixed type artificial endosperm: the soaked or stirring making by film forming agent, then murphy juice composition and film forming agent are mixed, film forming agent adopts the water-loss reducer of 7ml, and preferably, film forming agent adopts the mixture of 9 one-tenth water-loss reducers and 1 one-tenth essence of loosening the soil.
The second medium comprises nutritive element mother liquor, plant growth regulator mother liquor, insecticide, anti-browning agent, anti-pollutant, carbohydrate, yeast juice and mixed type artificial endosperm needed for moss growth.Nutritive element mother liquor needed for moss growth: prepare with KNOP, potassium nitrate 250mg/L, potassium dihydrogen phosphate 250mg/L, nitrate of lime 1000mg/L, magnesium sulfate 250mg/L, zinc sulphate 3mg/L.Plant growth regulator mother liquor is prepared with following ratio: 2,4-D is by 0.1moL NaOH warm water hydrotropy solution and the concentration of 2,4-D is 0.5-1mg/L, DA-6 diethyl aminoethyl hexanoate 0.5-3mg/L warm water hydrotropy solution.Insecticide is first just reaching sharp victory 1:10000 (pesticide-seeds ratio), and carbohydrate is as white sugar 30g/L, and Angel Yeast 0.32g/L, ascorbic acid 2mg/L, active carbon 0.7 gram/L, in order to play the effect of BPH resistant rice variety, antipollution, sustained release agent in the process of growth of liver moss.Film forming agent adopts 3.5ml water-loss reducer, and preferably, film forming agent adopts 9 one-tenth water-loss reducers and 1 one-tenth smart mixture that loosens the soil.
Preparation liver moss stoste:
First, nutritive element mother liquor needed for above-mentioned moss growth, plant growth regulator mother liquor, insecticide, anti-browning agent, antipollution, carbohydrate, yeast juice are mixed in a container, and add water in this container and make the temperature of liquid be 35 DEG C ~ 45 DEG C, secondly, in agitator, stir 1-2 minute together with the liver moss raw material through preliminary treatment, then mixed type artificial endosperm is put into agitator and stir 1-2 minute.Preferred gametophytic broken segment length of moulding bent tail moss is 0.1-0.2cm more.Again, in agitator, add pH value regulator is neutral with adjust ph or faintly acid, obtains liver moss stoste.
3rd step, is transferred to the green carving skeleton of sterilizing by the liver moss stoste of preparation.
First, after the first soaked cleaning of green carving skeleton, put into the sterilized solution timing sterilization of constant volume or adopt solar radiation to carry out sterilizing, after illumination sterilizing, green carving skeleton can keep dry state, and is more conducive to the transfer operation carrying out liver moss stoste.Secondly, then use the green carving skeleton of clean water, make green carving skeleton keep moisture state.Finally, liver moss stoste is brushed on green carving skeleton, preferably, make the liver moss stoste of brushing in green carving skeleton surface reach the state of not landing.In the present embodiment, transfer method adopts brushes, and can adopt any one or more mode in spraying, brushing, dip-coating during practical operation.
4th step, is positioned over the green carving skeleton being coated with described liver moss stoste in breeding device.
In the present embodiment, breeding induction period device is by breeding box, mesh-supported frame and the breeding cover made by light transmissive material, siron board constant temperature electric heater is formed, the cover top of described breeding cover is provided with air-vent, water is filled in described breeding box, the erection of described mesh-supported to be placed in described breeding box and away from the water surface, described green carving skeleton is positioned on described mesh-supported frame, described breeding cover is buckled and is located at described breeding box limit and buckles seam, described siron board batch (-type) constant temperature electric heater or similar action heater are positioned in the middle of at the bottom of breeding box, temperature is regulated to be water in 31 degree of heating cabinets, moulding place is 20-25 degree with temp. and humidity probe actual measurement cloudy or cloudy day.Breeding arrangement is located in Simple transparent film canopy.
5th step, moss cultivation is carried out in the growth direction controlling liver moss according to the film-forming state of the liver moss stoste being covered in green carving skeleton surface.
The film-forming state of the liver moss stoste on green carving skeleton surface is main control by the kind of film forming agent, component and starch component, described film forming agent is the compound of water-loss reducer or water-loss reducer and essence of loosening the soil, described medium contains starch component, and the growth direction of described liver moss is protonema.The film forming of green carving skeleton surface liver moss stoste judges feature: liver moss stoste covers green carving skeleton surface completely, and has reflective.In this example, film forming has reflective then can prevent germ contamination in cultivating process, and certain condensed water can be prevented, and the not landing in cultivation of liver moss stoste can be made, preserve nutrition, simultaneously because of the 20-25 DEG C that institute's controlling temperature is the growth of suitable protonema, film forming has reflective, and then film forming parcel liver moss seed is tight, liver moss bud cannot get out, and impels liver moss towards raw water pretreatment; This example film forming agent adopts compound to play to be beneficial to and stir into gelatification, water-loss reducer in stirring can not be flashed machine perisporium, and cleaning robot is easier; Water-loss reducer film can be broken through after raw water pretreatment and be presented on green carving surface.
Principal character in cultivating process: the viridescent protonema in green carving skeleton surface produces.The cultivating process of protonema comprises protonema induction period and protonema seeding stage.
Protonema induction period: time 20-35 days, control temperature 18-25 DEG C except fine day, be preferably 18-22 DEG C, intensity of illumination is 0-8000LX, humidity 75-97%.Fine day controls the temperature at green carving skeleton place within 30 DEG C, and within one week, can carry out a fine day illumination in interval, intensity of illumination controls at below 12000LX, and continuous sunny need do process of sheltering from heat or light, cloudy without the need to sheltering from heat or light.
Protonema grows and comprises one of following two kinds of breeding methods seedling stage: the first cultivating method: green carving surface presentation is thallus, protonema, cormus, callus four kinds of states.Film forming agent large usage quantity in mixed type artificial endosperm, breeding device is transferred to outdoor without heating cultivation, temperature 5-25 DEG C, illumination control 0-25000LX, humidity 60-95%, wherein the nurturing an environment humidity of thallus, protonema state is preferably 70-80%, and temperature is preferably 15-25 DEG C, and this Differentiation Types adopts the first medium.This type medium green carving skeleton proceeds in the reflective breeding device of buckle-type, and along with naturally strengthening of illumination in spring, pick up shady control as 0-15000LX, green carving can be divided into for thallus and protonema coexistence phase naturally, can pick up shady control and emerge for 0-3500LX helps after this stage; Separately the green carving after induction period is proceeded in the reflective breeding device of split, and bracing frame is level at the bottom of 10 centimetres high green carving skeletons together with water, can have and necessarily pass water function, illumination control 0-35000LX, green carving can be divided into cormus, thallus, callus, protonema four kinds of states naturally, and four kinds of states co-exist actual productions generally need not.Occur that callus reason is that temperature is too high, transfer callus to again by protonema.Also can there is another variation characteristic in this example: cause induction period because illumination is too low after, the surperficial redgreen protonema of green carving produces, after being transferred to outdoor nothing heating breeding device equivalent environment equally, still can present described four kinds of states.Second cultivating method: protonema is divided into thallus state, in mixed type artificial endosperm, film forming agent consumption is less, reflective for buckle-type breeding device is transferred to outdoor without heating cultivation, illumination control 0-4500LX, within time 25-45 days, promote the further development growth of protonema, and being beneficial to minimizing water spray number of times, the 0-3500LX of illumination control afterwards, is preferably 1200-2500LX.Protonema grows temperature 5-25 DEG C in seedling stage, and be preferably 18-22 degree, humidity 60-90% is preferably 70-80%, and this Differentiation Types adopts the second medium.Second cultivating method is emerged denser, and maintenance water spray number of times is less.
This example favorable season in natural environment is play breeding December in winter.Can not supplement the nutrients in this example cultivating process.Also pigment can be added in this example, in order to change liver moss color in medium.In this example artificial endosperm can by sodium alginate add tapioca starch do slightly poor substitute.Also by this example water control temp. and humidity principle, therefore in batch production, breeding arrangement can with being incubated the long tank of breeding, bracing frame, reflector, reflective membrane, large-scale heater, and breeding cover is formed.Breeding cover size is in order to purchase or make or operate and determine, and large-scale heater is according to the required heating water yield and temperature sizing number, and reflective membrane and reflector size radian are fixed with breeding cover.
Embodiment three
The present embodiment provides preparation method the method for a kind of moss green carving to comprise the following steps:
The first step, carries out preliminary treatment by the liver moss raw material of collection: first, and the first cultivating method collection moss is crawled and lied prostrate the raw gametophyte doing the blue or green moss represented of sheet; Second cultivating method collection moss is uprightly grown thickly and is made the gametophyte of the white hair moss represented; Then clean to remove the impurity such as earth, rubble, withered grass leaf with water, then clean with washing powder and water.Finally being extracted by the water in liver moss raw material makes the gametophyte of liver moss be moisture state.
Second step, mixes liver moss stoste by the medium needed for moss growth and by the liver moss raw material after step (1) processes:
Medium: the first cultivating method medium comprises nutritive element mother liquor, plant growth regulator mother liquor, bactericidal agent, insecticide, anti-browning agent, anti-pollutant, carbohydrate, yeast juice and single type artificial endosperm needed for moss growth.Particularly, the nutritive element mother liquor needed for moss growth: prepare with KNOP, potassium nitrate 250mg/L, potassium dihydrogen phosphate 250mg/L, nitrate of lime 1000mg/L, magnesium sulfate 250mg/L, zinc sulphate 3mg/L.Plant growth regulator mother liquor is prepared with following ratio: 2,4-D 0.1moLNaOH warm water hydrotropy solution, 2,4-D concentration be 0.25mg/L, 6-BA0.25mg/L 0.1mol NaOH warm water hydrotropy solution, multiple nitre confused sodium 1.0-1.5mg/L warm water hydrotropy solution.Adopt 2,4-D of 0.25mg/L liver moss raw material can be induced to produce without callus, green carving surface has bud to produce; Bactericide is first just reaching bright shield 1:1200 (pesticide-seeds ratio), insecticide is first just reaching sharp victory 1:12000 (pesticide-seeds ratio), carbohydrate as white sugar 12g/L, ascorbic acid 2mg/L BPH resistant rice variety, Angel Yeast 0.32g/L, active carbon 0.7g/L are used as antipollution, the sustained release agent of blue or green moss.Single type artificial endosperm passes through to be made soaked for film forming agent, film forming agent essence of loosening the soil.
Second cultivating method medium comprises nutritive element mother liquor, plant growth regulator mother liquor, bactericidal agent, insecticide, anti-browning agent, anti-pollutant, carbohydrate, yeast juice and single type artificial endosperm needed for moss growth.Particularly, the nutritive element mother liquor needed for moss growth: prepare with KNOP, potassium nitrate 250mg/L, potassium dihydrogen phosphate 250mg/L, nitrate of lime 1000mg/L, magnesium sulfate 250mg/L, zinc sulphate 3mg/L.Plant growth regulator mother liquor is prepared with following ratio: 2,4-D 0.1moLNaOH warm water hydrotropy solution, 2, the concentration of 4-D is 0.5mg/L, and bactericide is first just reaching bright shield 1:1200 (pesticide-seeds ratio), and insecticide is first just reaching sharp victory 1:12000 (pesticide-seeds ratio), carbohydrate is as white sugar 30g/L, ascorbic acid 2mg/L BPH resistant rice variety, Angel Yeast 0.32g/L, diatomite 0.7g/L are used as antipollution, the sustained release agent of white hair moss.6-BA0.25mg/L 0.1mol NaOH warm water hydrotropy solution, multiple nitre confused sodium 1-1.5mg/L warm water hydrotropy solution.Single type artificial endosperm passes through to be made soaked for film forming agent, film forming agent essence of loosening the soil.
The present embodiment first cultivating method and the second cultivating method medium are for moss kind factory formula, therefore each constituent content of in medium numerous because of liver moss kind can carry out increasing or reducing as the case may be, the usage ratio of each component in medium is not construed as limiting, but at least should containing above-mentioned KNOP nutritive element, plant growth regulator, carbohydrate, film forming agent.
Preparation liver moss stoste: first, the nutritive element mother liquor needed for above-mentioned moss growth, plant growth regulator mother liquor, bactericide, insecticide, anti-browning agent, anti-pollutant, carbohydrate and yeast juice are mixed in agitator.Secondly, and in this agitator, add blue or green moss, temperature that water makes liquid is 25 DEG C, again, add single type artificial endosperm and cooperatively stir 0.5-1 minute in agitator, preferably, the gametophytic broken segment length of liver moss is 0.2 ~ 0.3cm is main.Finally, the pH value of regulates liquid is neutral or faintly acid, obtains liver moss stoste.
3rd step, is transferred to the green carving skeleton of sterilizing by the liver moss stoste of preparation, this example to be the length of green carving skeleton be 15cm, wide 10cm, high 12 centimetres.
First, after the first soaked cleaning of green carving skeleton, put into the sterilized solution timing sterilization of constant volume or adopt solar radiation to carry out sterilizing, after illumination sterilizing, green carving skeleton can keep dry state, and is more conducive to the transfer operation carrying out liver moss stoste.Secondly, then use the green carving skeleton of clean water, make green carving skeleton keep moisture state.Finally, liver moss stoste is brushed on green carving skeleton, preferably, make the liver moss stoste of brushing in green carving skeleton surface reach the state of not landing.Green carving skeletal back recess, transplants simultaneously and has the strain of high 10 centimetres of Selaginella tamariscinas 1.
4th step, is positioned over the green carving skeleton being coated with described liver moss stoste in breeding device.
In the present embodiment, breeding device is made up of breeding box, mesh-supported frame, reflective membrane, the breeding cover made by light transmissive material, circular arc reflector.The cover top of described breeding cover is provided with air-vent, water is filled in described breeding box, the erection of described mesh-supported to be placed in described breeding box and away from the water surface, described reflective membrane is layed on bracing frame, described green carving skeleton is positioned on described mesh-supported frame reflective membrane, and described breeding cover is buckled and is located in described breeding box and stretches under the water surface.The long 65cm of breeding box, wide 38cm, high 15 centimetres, the long 54cm of breeding cover, wide 28cm, high 25 centimetres, mesh-supported frame, apart from the water surface 3 centimetres, bracing frame is equipped with reflective membrane.In described circular arc reflector, arc plate is that plastic not easy rusting material is made, as metallic plate or plastic plate, this adopts 0.2 millimeters thick aluminium zine plate, make flanging and strengthen latter long 45 centimetres wide 15 centimetres of process, be converted into circular arc reflector again, illumination is simpler and easy picks up under cool canopy or tree shade directly reflective by aluminium zine plate true qualities, and illumination is more weak simple and easyly picks up bonding or fixing reflective with aluminium zine plate size reflective membrane with clip on aluminium zine plate under cool canopy or tree shade; The reflective board of circular arc be 70 degree by being located at breeding box limit and forming reflecting angle.The reflective breeding device of described split is not limited to this example specification.In order to know the temperature and humidity in the reflective breeding device of split, to carry out adjustment temperature and humidity.Be provided with Hygrothermograph in breeding box, utilize the illuminance in hand-held illuminometer determinator.This example breeding device summer simple and easy pick up cool canopy or tree shade under compare other breeding device and can to lower the temperature 3-5 degree more.
Additionally provide a kind of green carving skeleton in the present embodiment, it comprises metal net layer 101, surface layer 102 and suture 103, consults Fig. 2.Surface layer 102 is laid on metal net layer 101, and metal net layer 101 and surface layer 102 are sewed up fixing by described suture 103.Metal net layer 101 has good plasticity, and green carving skeleton is collapsible, thus solves common green carving frame configuration and fix, the defect of not collapsible change.When making green carving skeleton, first metal net layer 101 and surface layer 102 is chosen, by suture 103, metal net layer 101 and surface layer 102 are sewed up, metal net layer 101 and surface layer 102 are fit together closely, by paper folding method, the green carving skeleton sewed up is folded into various shape.On the other hand, surface layer 102 as the attachment carrier for cultivating the plants such as such as liver moss, can realize on green carving skeleton, cultivate moss green carving and forming various moss green carving moulding.Surface layer 102 need be beneficial to the attachment of liver moss stoste and moss growth, such as 3M scouring pad, certainly also can by the geotextiles, air filtering cellucotton etc. of other class material as fibrous material.Suture 103 preferably adopts nylon wire, and nylon wire elasticity is strong and durable.
5th step, moss cultivation is carried out in the growth direction controlling liver moss according to the film-forming state of the liver moss stoste being covered in green carving skeleton surface.
The film-forming state being covered in the liver moss stoste on green carving skeleton surface is main control by the kind of film forming agent and component.Main control refers to kind and the component that the factor playing main, crucial control action to the film-forming state of the liver moss stoste on green carving skeleton surface is film forming agent, the content of other the composition in medium, the size of liver moss raw material and consumption also also can play auxiliary control action to the film-forming state of liver moss stoste, but what play a major role is kind and the component of film forming agent.Wherein film forming agent adopts essence of loosening the soil, this brand used is " Heze section of Switzerland strangely-shaped pines soil essence ", essence of loosening the soil is not limited to this brand used, when same liver moss mix and blend, having expands forms spumescence, therefore longer with the broken segment length of time controling in same machine, the longer then vitality of broken section of liver moss is stronger; Liver moss stoste turns liquid to be had adhesiveness and becomes film on skeleton, cultivates whole process not easily landing, and can jointly play certain stored nutrient with the liver moss raw material of process of anhydrating and be beneficial to growth; When liver moss stoste on skeleton is accidentally dry, when adopting spraying or immersion moisturizing, rapid absorption can be beneficial to and keep the skin wet, and can not like gel-like moisturizing difficulty; And be that water adhesion agent has like accessibility as water because of it, therefore very light to the pressure of liver moss, be beneficial to photosynthetic, liver moss emerges lighter.Preferably, can also assist and be controlled to membrane stage by controlling weight in liver moss stoste of water content in medium, blue or green moss or white hair moss and the broken segment length of liver moss.
Described film forming agent is essence of loosening the soil, the not starch-containing composition of described medium, and breeding method comprises the first cultivating method and the second cultivating method, and it is with the numerous bud of bud that described first cultivating method grows direction; It is protonema that described second cultivating method grows direction; The film forming of green carving skeleton surface liver moss stoste judges feature: liver moss stoste covers green carving skeleton surface completely.
Breeding method comprises the first cultivating method and the second cultivating method.
First cultivating method comprises with bud numerous bud inducement phase with bud numerous bud seeding stage:
Principal character in first cultivating method cultivating process: green carving skeleton surface has young moss seedlings to produce.First cultivating method medium crawls the raw class liver moss of volt sheet as blue or green moss 2,4-D suitable concentration is 0.25mg/L, carbohydrate is suitably 8g/L ~ 12g/L, class of uprightly growing thickly liver moss lignification higher as white hair moss NAA (a-methyl α-naphthyl acetate) or a-Nafusaku suitable concentration be 0.01mg/L, GA3 suitable concentration is 1mg/L, carbohydrate is suitably 28g/L ~ 32g/L, and this Differentiation Types adopts the first medium.
Second cultivating method comprises protonema induction period, protonema seeding stage.Principal character in second cultivating method cultivating process: green carving skeleton surface has a large amount of protonema to produce, and produces with a small amount of young moss seedlings; Second cultivating method medium white hair moss 2,4-D suitable concentration is that 0.5mg/L carbohydrate is suitably 28g/L ~ 32g/L, blue or green moss 2,4-D suitable concentration is 0.1mg/L, and carbohydrate is suitably 8g/L ~ 12g/L;
With bud numerous bud inducement phase: time 20-30 days, temperature 5-28 DEG C, be preferably 15-25 DEG C, illumination below control 3500LX, be preferably 1200-2500LX, humidity 60-90%, be preferably 70-80%;
With bud numerous bud seeding stage: temperature 5-28 DEG C, be preferably 15-25 DEG C, illumination below control 3500LX, be preferably 1200-2500LX, humidity 60-90%, be preferably 70-80%;
Protonema induction period: time 25-45 days, temperature 18-25 DEG C, be preferably 20-25 DEG C, illumination controls 0-8000LX, preferably 3500-4500LX, humidity 60-90% is preferably 70-80%;
In the protonema seeding stage: temperature 5-28 DEG C, be preferably 15-25 DEG C, illumination below control 3500LX, be preferably 1200-2500LX, humidity 60-90%, be preferably 70-80%;
First cultivating method crawls volt sheet raw class bryophyte growth regulator formation also can be as follows: formula one, 2,4-D0.25mg/L, diethyl aminoethyl hexanoate 1-2mg/L; Formula two, 2,4-D0.25mg/L, the multiple confused sodium 1-1.5mg/L of nitre, formula three, 6-BA0.5-1mg/L, a-Nafusaku 0.25-0.5mg/L, 3-butyric acid potassium 0.25mg/L, diethyl aminoethyl hexanoate 1mg/L etc., or add chlormequat etc. again and all can be used for producing.First cultivating method uprightly grow thickly class liver moss formula one, NAA0.01mg/L, GA3 gibberellin 1mg/L, diethyl aminoethyl hexanoate 2-3mg/L; Formula two, NAA0.01mg/L, GA3 gibberellin 1mg/L, the multiple confused sodium 2-3mg/L of nitre etc. can be used for producing.When plant growth regulator excessive concentration in first cultivating method, as adopted 2, when 4-D1mg/L cultivates blue or green moss, also another feature can be rendered as: green carving skeleton surface has young moss seedlings to produce in cultivating process, derivative liver moss raw material can be rendered as bottle green callus, can be converted into protonema and be divided into seedling again, in this feature, to turn protonema more difficult for callus, generally need not.
In addition, the plant growth regulator of uprightly growing thickly in medium can adopt as follows: the concentration of formula one, 2,4-D is 0.5mg/L; The concentration of formula two, 2,4-D is 0.5mg/L, and diethyl aminoethyl hexanoate 1-2mg/L etc. can be used for producing.Volt sheet raw class bryophyte growth regulator of crawling is formed also can be as follows: the concentration of formula one, 2,4-D is 0.1mg/L; The concentration of formula two, 2,4-D is 0.1mg/L, and diethyl aminoethyl hexanoate 0.5-1mg/L etc. can be used for producing.
First and second cultivating methods reach aseptic without worm operation in greenhouse or group training indoor, and medium can not with bactericidal agent and insecticide.
The favorable season of this example in natural environment is the cultivation breeding carrying out liver moss in spring, summer and autumn, and protonema induction need be aided with temperature control measure in summer and reach.1/2 concentration cultures composition can be adopted before liver moss emerges once to make nutritional supplementation with spray-on process every 10-15 days.
By this example water control temp. and humidity cool principle, therefore in batch production, breeding arrangement is the long tank of available insulation breeding also, bracing frame, reflector, reflective membrane, pipeline and circulating water pump device, air-supply arrangement, and multiple breeding cover is formed.Breeding cover size is in order to purchase or make or operation and determine, and reflective membrane and reflector size radian are fixed with breeding cover, and pipeline and circulating water pump device, air-supply arrangement to be shaped number according to institute's water requirement and amount of moisture.
Embodiment four
The present embodiment provides preparation method the method for a kind of moss green carving to comprise the following steps:
The first step, carries out preliminary treatment by the liver moss raw material of collection: first, and collection moss is crawled and lied prostrate the raw gametophyte doing the blue or green moss represented of sheet, then cleans to remove the impurity such as earth, rubble, withered grass leaf with water, then cleans with washing powder and water.Finally being extracted by the water in liver moss raw material makes the gametophyte of blue or green moss be moisture state.
Second step, mixes liver moss stoste by the medium needed for blue or green moss moss growth and by the liver moss raw material after step (1) processes:
Medium: medium comprises nutritive element mother liquor, plant growth regulator mother liquor, insecticide, anti-browning agent, antipollution, carbohydrate, yeast juice and mixed type artificial endosperm needed for moss growth.Particularly, the nutritive element mother liquor needed for moss growth: prepare with KNOP, potassium nitrate 250mg/L, potassium dihydrogen phosphate 250mg/L, nitrate of lime 1000mg/L, magnesium sulfate 250mg/L, zinc sulphate 3mg/L.Plant growth regulator mother liquor is prepared with following ratio: 6-BA 0.5-6mg/L 0.1moL NaOH warm water hydrotropy solution, a-Nafusaku 0.25-3mg/L warm water hydrotropy solution, 3-butyric acid potassium 0.25-1.5mg/L warm water hydrotropy solution, DA-6 diethyl aminoethyl hexanoate 1-6mg/L warm water hydrotropy solution.Bactericide is first just reaching bright shield 1:1000 (pesticide-seeds ratio), insecticide chitosan 1:600 (pesticide-seeds ratio), carbohydrate as white sugar 12g/L, ascorbic acid 2mg/L, Angel Yeast 0.32g/L mother liquor, active carbon 0.7 gram/L.Starch component and film forming agent, by by soaked for film forming agent making, then mix together by mixed type artificial endosperm.Wherein, the first cultivating method film forming agent essence of loosening the soil, starch component is cut the potato fritter of fritter through peeling.Second cultivating method film forming agent sodium alginate or preferably employing 8 one-tenth sodium alginates are with 2 one-tenth smart compounds that loosen the soil, and starch component is tapioca starch.
Preparation liver moss stoste: first, the nutritive element mother liquor needed for above-mentioned moss growth, plant growth regulator mother liquor, insecticide, anti-browning agent, anti-pollutant, carbohydrate and yeast juice are mixed in agitator.Secondly, and in this agitator, add blue or green moss, temperature that water makes liquid is about 25 DEG C, again, in agitator, add mixed type artificial endosperm stir 1-2 minute together, preferably, the gametophytic broken segment length of blue or green moss is 0.25-0.35cm is main, and the first cultivating method film forming agent starch based is such as without filtering potato grain, juice, and wherein to reach diameter, to be about 0.1cm be good to potato grain; Second cultivating method film forming agent, preferably, adopt 8 one-tenth sodium alginates with 2 one-tenth smart compounds that loosen the soil, starch component is tapioca starch.
Finally, the pH value of regulates liquid is 6.8-7, obtains liver moss stoste.
3rd step, is transferred to the green carving skeleton of sterilizing by the liver moss stoste of preparation, the length of this example green carving skeleton is 15cm, wide 7cm, high 12 centimetres.
First, after the first soaked cleaning of green carving skeleton, put into the sterilized solution timing sterilization of constant volume or adopt solar radiation to carry out sterilizing.Secondly, then use the green carving skeleton of clean water, make green carving skeleton keep moisture state.Finally, liver moss stoste is brushed on green carving skeleton, preferably, make the liver moss stoste of brushing in green carving skeleton surface reach the state of not landing.
4th step, is positioned over the green carving skeleton being coated with described liver moss stoste in breeding device.
In the present embodiment, the reflective breeding device of buckle-type is made up of buckle-type breeding box, mesh-supported frame and the breeding closure assembly made by light transmissive material, seedling-cultivating tray, circular arc reflector.Described buckle-type breeding box is that 2 centimetres of plastic feets form circular breeding basin and utilize white true qualities reflective by white diameter 30 centimetres, height, further the more weak place of illumination also can at the bottom of breeding basin and all sides lay reflex film, transparent plastic circle flat-top diameter 27 centimetres, high 15 centimetres form breeding covers; The cover top flat-top sidepiece of described breeding cover is provided with air-vent; Described breeding box basin inner drying fills water season, and overcast and rainy season is not filled with water; The erection of described mesh-supported is placed in described breeding box basin and away from water surface 3cm, described green carving skeleton is positioned on described mesh-supported frame, and described breeding cover is buckled and is located at Kou Fengpen limit, described breeding box limit and buckles seam.Described seedling-cultivating tray is plastic material is square, and bottom is the cribriporal that gathers, and diameter 39 centimetres is high 5.5 centimetres, top diameter 42.5 centimetres.
In described circular arc reflector, arc plate is that plastic not easy rusting material is made, as metallic plate or plastic plate, this adopts 0.2 millimeters thick aluminium zine plate, make flanging and strengthen latter long 45 centimetres wide 13 centimetres of process, be converted into circular arc reflector again, directly reflective by aluminium zine plate true qualities under the stronger tree shade of illumination, bonding or fixing reflective with aluminium zine plate size reflective membrane with clip on aluminium zine plate under the more weak tree shade of illumination; The circular arc reflector left and right sides at the bottom of limit 12 centimetres, high 1 centimeters respectively opens 2 diameters, 1 centimetre of aperture, at a distance of 2 centimetres; Circular arc reflector is apart from seedling-cultivating tray 2 centimetres on one side, card also by being located at nursery web, adopting rope perforation reflector and seedling-cultivating tray to be fixed, regulating reflecting angle to be 75 degree, adjusting angle can directly block by being formed, and also can add gravelstone or wooden unit support adjusting angle after reflector.
The reflective breeding device of described buckle-type is not limited to this example specification, shape, such as, in this example, in the reflective breeding device of buckle-type, breeding cover also can be spheroidal, breeding box and cover also can be rectangular arch etc., select the corresponding reflective breeding device of buckle-type, seedling-cultivating tray, the circular arc reflector that are applicable to green carving moss growth according to green carving skeleton specification is adjustable; Also can the material such as plastics to add reflectorized material overall or point body formed, occur to meet the described buckle-type reflective breeding apparatus function form of the composition, do not limit size, shape, but there is certain aesthetic property, cultivate green carving for family DIY or sightseeing type factory cultivates green carving.The reflective breeding device of buckle-type serves free adjustment light filling angle and multi-angle light filling, and without the need to regularly exchanging the effect of green carving light angle, rainproof, anti-ponding, profit growth, sharp transportation again, is applicable to the green carving of outdoor large scale cultivating.And indoor are without the need to rainwater-proof, in the reflective breeding device of buckle-type: breeding cover can select the top of dome to open air-vent, seedling-cultivating tray can bottom atresia.
Buckle-type reflective breeding device is also applicable to following breeding method except this example: be applicable to the not starch-containing composition of described medium, described growth direction is for cultivate green carving with the numerous bud of bud; The growth direction of described liver moss is that protonema cultivates green carving; The growth direction of described liver moss is that callus cultivates green carving.
5th step, moss cultivation is carried out in the growth direction controlling liver moss according to the film-forming state of the liver moss stoste being covered in green carving skeleton surface.
The film-forming state being covered in the liver moss stoste on green carving skeleton surface is main control by the kind of film forming agent and component and starch based composition, preferably, assist be controlled to membrane stage by controlling volume in liver moss stoste of water content in medium, blue or green moss and weight and the broken segment length of blue or green moss.Be used as the film-forming state of liver moss stoste for major control factors with the kind of film forming agent, component and starch based composition in the present embodiment, its concrete consumption, kind are studied.Described growth direction is with the numerous bud of bud.First cultivating method film forming agent essence of loosening the soil, starch component is murphy juice, grain.Second cultivating method film forming agent adopts 8 one-tenth sodium alginates with 2 one-tenth smart compounds that loosen the soil, and starch component is tapioca starch.This example two kinds of cultivating method mixed type artificial endosperm application targets are all make skeleton surface stoste state be rendered as further to have certain honeycomb cavernous structure, ease off the pressure, increase gas permeability, make to emerge lighter, and reduce because of adding of starch material in cultivation and spray nutrition number of times, even until greenly carve product all without the need to supplementing the nutrients.
Film forming judges feature: liver moss stoste covers green carving skeleton surface completely;
, in this breeding method, there are one of following two kinds of features in principal character in cultivating process:
First cultivating method feature: liver moss stoste was transferred to green carving skeleton surface after 1-3 days, be exposed to illumination and assign comprehensively dry to carry out sterilizing, green carving skeleton surface has young moss seedlings to produce;
Second cultivating method feature: without the need to being exposed to sterilizing under illumination after liver moss stoste is transferred to green carving skeleton surface, green carving skeleton surface has young moss seedlings to produce;
Comprise with bud numerous bud inducement phase with bud numerous bud seeding stage with bud numerous bud cultivating process;
One of following two kinds of breeding methods are comprised with bud numerous bud inducement phase:
First cultivating method: liver moss stoste was transferred to green carving skeleton surface after 1-3 days, was preferably after 2 days, is then exposed to illumination and assigns comprehensively dry to carry out sterilizing, and then soak or spray water and put shady place, cultivate 20-30 days continuously;
Second cultivating method: after liver moss stoste is transferred to green carving skeleton surface, cultivates 20-30 days continuously;
The breeding condition of the first cultivating method and the second cultivating method is temperature 5-28 DEG C, is preferably 15-25 DEG C, illumination below control 3500LX, is preferably 1200-2500LX, humidity 60-90%, is preferably 70-80%;
With bud numerous bud seeding stage: temperature 5-28 DEG C, be preferably 15-25 DEG C, illumination below control 3500LX, be preferably 1200-2500LX, 1-2 time weekly, early or/and in 1 hour evening below 15000-25000LX sunlight help growth, humidity 60-90%, be preferably 70-80%.Early, carry out intense light irradiation in short-term evening can rise in cultivating process because helping sterilization functions containing organic matter.This example cultivation season suitable in natural environment is spring, autumn.
Embodiment five
The present embodiment provides preparation method the method for a kind of moss green carving to comprise the following steps:
The first step, carries out preliminary treatment by the liver moss raw material of collection: first, and collection moss is uprightly grown thickly and made the gametophyte of the white hair moss represented, and then cleans to remove the impurity such as earth, rubble, withered grass leaf with water, then cleans with washing powder and water.Finally the water in liver moss raw material being extracted the gametophyte making white hair moss is moisture state.
Second step, mixes liver moss stoste by the medium needed for the growth of white hair moss with by the liver moss raw material after step (1) processes:
Medium: comprise the nutritive element mother liquor needed for moss growth, plant growth regulator mother liquor, insecticide, anti-browning agent, anti-pollutant, carbohydrate, yeast juice and single type artificial endosperm.Particularly, the nutritive element mother liquor needed for moss growth: prepare with KNOP, potassium nitrate 250mg/L, potassium dihydrogen phosphate 250mg/L, nitrate of lime 1000mg/L, magnesium sulfate 250mg/L, zinc sulphate 3mg/L.Plant growth regulator mother liquor is prepared with following ratio: 6-BA 1-6mg/L 0.1moL NaOH warm water hydrotropy solution, a-Nafusaku 0.5-2mg/L warm water hydrotropy solution, 3-butyric acid potassium 0.25-1mg/L warm water hydrotropy solution, compound sodium nitrophenolate 1-6mg/L warm water hydrotropy solution.Bactericide is first just reaching bright shield 1:1200 (pesticide-seeds ratio), insecticide chitosan 1:800 (pesticide-seeds ratio), carbohydrate as white sugar 30g/L, ascorbic acid 2mg/L, Angel Yeast 0.32g/L, active carbon 0.7-1.2g/L.Mixed type artificial endosperm is made by film forming agent is soaked or stirring, then starch component and film forming agent are mixed, the compound of film forming agent water-loss reducer or water-loss reducer and essence of loosening the soil, water-loss reducer is 9:1 with the mass ratio of essence of loosening the soil, and starch component is without filtering murphy juice.
Preparation liver moss stoste: first, the nutritive element mother liquor needed for above-mentioned moss growth, plant growth regulator mother liquor, insecticide, anti-browning agent, anti-pollutant, carbohydrate and yeast juice are mixed in agitator.Secondly, and make the temperature of liquid be 25 DEG C to Jia Baimao moss, water in this agitator, stir 1 minute.Again, add mixed type artificial endosperm and stir 1-2 minute in agitator, preferably, the gametophytic broken segment length of white hair moss is 0.1cm.Finally, the pH value of regulates liquid is 6-7, is preferably 6.8-7, obtains liver moss stoste.
3rd step, is transferred to the green carving skeleton of sterilizing by the liver moss stoste of preparation, this example to be the length of green carving skeleton be 15cm, wide 10cm, high 12 centimetres.
First, after the first soaked cleaning of green carving skeleton, put into the sterilized solution timing sterilization of constant volume or adopt solar radiation to carry out sterilizing, after illumination sterilizing, green carving skeleton can keep dry state, and is more conducive to the transfer operation carrying out liver moss stoste.Secondly, then use the green carving skeleton of clean water, make green carving skeleton keep moisture state.Finally, liver moss stoste is brushed on green carving skeleton, preferably, make the liver moss stoste of brushing in green carving skeleton surface reach the state of not landing.
4th step, is positioned over the green carving skeleton being coated with described liver moss stoste in breeding device.
In the present embodiment, breeding device: be made up of breeding box, mesh-supported frame and the breeding cover that made by light transmissive material, the cover top of described breeding cover is provided with air-vent, water is filled in described breeding box, the erection of described mesh-supported to be placed in described breeding box and away from the water surface, described green carving skeleton is positioned on described mesh-supported frame, and described breeding cover is buckled and is located at described breeding box limit and buckles seam.The long 54cm of breeding box, wide 28cm, high 6 centimetres, mesh-supported frame is apart from the water surface 3 centimetres.The long 47cm of mesh-supported frame, wide 23cm, high 3cm.
5th step, moss cultivation is carried out in the growth direction controlling liver moss according to the film-forming state of the liver moss stoste being covered in green carving skeleton surface.
Film-forming state is main control by the kind of film forming agent, component and starch component, preferably, assists be controlled to membrane stage by controlling volume in liver moss stoste of water content in medium, white hair moss and weight and the white broken segment length of hair moss.Described film forming agent is the compound of water-loss reducer or water-loss reducer and essence of loosening the soil, and described medium contains starch component, and described growth direction is callus;
Film forming judges feature: liver moss stoste covers green carving skeleton surface completely and has certain reflective; In this example, there is reflective and then can prevent germ contamination in cultivating process, and certain condensed water can be prevented, and the not landing in cultivation of liver moss stoste can be made, preserve nutrition, simultaneously therefore large to liver moss pressure, force it to grow towards callus direction in hot and humid situation; This example film forming agent adopts compound to play to be beneficial to and stir into gelatification, water-loss reducer in stirring can not be flashed machine perisporium, and cleaning robot is easier.Broken section controls thinner, is beneficial to and makes callus easily be divided into protonema, and can occur a small amount of liver moss seedling.Certainly in this example, if broken section of liver moss controls also can ensure that Cultivated objective unification is out callus entirely at 0.2-0.35 centimetre, but differentiation is more difficult, therefore does not generally adopt.
Principal character in cultivating process: after induction period, green carving skeleton surface has a large amount of callus and a small amount of bud to produce; Callus cultivating process comprises the callus induction phase, callus turns protonema and the protonema seeding stage;
The callus induction phase: time 20-30 days, temperature 20-30 DEG C, illumination below control 2500LX, humidity 70-90% is preferably 80-90%;
Callus turns the protonema phase: time 25-45 days, temperature 15-25 DEG C, is preferably 18-22 DEG C, illumination control 3500-15000LX, and be preferably 3500-8000LX, humidity 60-90% is preferably 70-80%.
In the protonema seeding stage: temperature 5-28 DEG C, be preferably 15-25 DEG C, illumination below control 3500LX, be preferably the illumination receive direction that 1200-2500LX, every 15-20 days change green carving skeleton, humidity 60-90% is preferably 70-80%.
This example favorable season in natural environment is play breeding in 9 months autumns.This example once can supplement 1/2 concentration cultures nutrient component in 20-30 days before emerging.

Claims (10)

1. a preparation method for moss green carving, is characterized in that, comprises the following steps:
(1) the liver moss raw material of collection is carried out preliminary treatment;
(2) liver moss stoste is mixed by the medium needed for moss growth and by the liver moss raw material after step (1) processes;
(3) make green carving skeleton and described liver moss stoste be transferred to the green carving skeleton through sterilizing;
(4) the green carving skeleton being coated with described liver moss stoste is positioned in breeding device;
(5) control the growth direction of liver moss according to the film-forming state of the liver moss stoste being covered in green carving skeleton surface, carry out moss cultivation.
2. the preparation method of moss green carving according to claim 1, is characterized in that, the described preliminary treatment in step (1) comprises following operation:
(1.1) first impurity is removed to the liver moss raw material cleaning gathered;
(1.2) carrying out sterilization treatment to removing the liver moss raw material after impurity, then liver moss raw material being anhydrated process, making liver moss raw material be moisture state.
3. the preparation method of moss green carving according to claim 1, is characterized in that, the described medium in step (2) comprises following composition:
(2.1) the nutritive element mother liquor needed for moss growth, plant growth regulator mother liquor, insecticide, anti-browning agent, anti-pollutant, carbohydrate and yeast juice;
(2.2) single type artificial endosperm or mixed type artificial endosperm:
Single type artificial endosperm: by film forming agent soaked or stir be made; Mixed type artificial endosperm: the soaked or stirring by film forming agent, then mixes starch component and film forming agent.
4. the preparation method of moss green carving according to claim 3, is characterized in that, the described preparation liver moss stoste in step (2) comprises multi-step mixing facture or synchronously stirs facture:
Multi-step mixing facture: be mixed in container by each component in step (2.1), adds water and makes the temperature of liquid be 20 DEG C ~ 45 DEG C, then put into agitator together with the liver moss raw material through preliminary treatment and stir 1-2 minute in container; Again the single type artificial endosperm of making or mixed type artificial endosperm are put into agitator, then stir 1-2 minute; In agitator, add pH value regulator is neutral with adjust ph or faintly acid, obtains liver moss stoste;
Synchronous stirring facture: each component in step (2.1) is mixed in container, add water in container and make the temperature of liquid be 20 DEG C ~ 45 DEG C, then with through the liver moss raw material of preliminary treatment, single type artificial endosperm or mixed type artificial endosperm put into agitator, stir 0.5-2 minute; In agitator, add pH value regulator is again neutral with adjust ph or faintly acid, obtains liver moss stoste.
5. the preparation method of moss green carving according to claim 1, is characterized in that, the making green carving skeleton in step (3) the green carving skeleton be transferred to through sterilizing comprises following operation by described liver moss stoste:
(3.1) make green carving skeleton and by the first soaked cleaning of green carving skeleton, then put into the sterilized solution timing sterilization of constant volume or by illumination sterilizing, described green carving skeleton comprises metal net layer, surface layer and suture; Described surface layer is laid on described metal net layer, and described metal net layer and described surface layer are fixed by described suture;
(3.2) use the green carving skeleton after clear water drip washing sterilization treatment again, make green carving skeleton keep moisture state afterwards;
(3.3) liver moss stoste is transferred to the green carving skeleton in moisture state, make the liver moss stoste being covered in green carving skeleton surface reach not landing, the method for described transfer comprises any one or more in spraying, brushing, dip-coating.
6. the preparation method of moss green carving according to claim 1, is characterized in that, the described breeding device in step (4) comprises as one of lower device:
The reflective breeding device of buckle-type: be made up of breeding box, mesh-supported frame, reflex reflector and the breeding cover that made by light transmissive material, the cover top of described breeding cover or top, side are provided with air-vent, water is filled in described breeding box, the erection of described mesh-supported to be placed in described breeding box and away from the water surface, described green carving skeleton is positioned on described mesh-supported frame, and the side seam being located at described breeding box buckled by described breeding cover; Described reflex reflector is made up of reflective membrane and reflector, and described reflective membrane is layed in breeding box inwall and all sides, and described reflector is located at outside breeding box side;
The reflective breeding device of split: be made up of breeding box, bracing frame, reflex reflector and the breeding cover that made by light transmissive material, the cover top of described breeding cover or top, side are provided with air-vent, water is filled in described breeding box, support frame as described above to be arranged in described breeding box and away from the water surface, described green carving skeleton is positioned on support frame as described above, and described breeding cover is buckled and is located in described breeding box and stretches under the water surface; Described reflex reflector is made up of reflective membrane and reflector, and described reflective membrane is layed on bracing frame or is layed in breeding box inner circumferential side, and described reflector is located in breeding box or outside breeding box.
7. according to the preparation method of the moss green carving one of claim 1 to 6 Suo Shu, it is characterized in that, film-forming state described in step (5) is main control by the kind of film forming agent, component and starch component, described film forming agent is the compound of water-loss reducer or water-loss reducer and essence of loosening the soil, described medium contains starch component, and the growth direction of described liver moss is protonema;
Film forming judges feature: liver moss stoste covers green carving skeleton surface completely, and has reflective;
Principal character in cultivating process: the viridescent protonema in green carving skeleton surface produces;
The cultivating process of protonema comprises protonema induction period and protonema seeding stage;
Protonema induction period: time 20-35 days, control temperature 18-25 DEG C except fine day, intensity of illumination is 0-8000LX, humidity 75-97%; Fine day controls the temperature at green carving skeleton place within 30 DEG C, and within one week, can carry out a fine day illumination in interval, intensity of illumination controls at below 12000LX, and continuous sunny need do process of sheltering from heat or light, cloudy without the need to sheltering from heat or light;
Protonema grows and comprises one of following two kinds of breeding methods seedling stage:
First cultivating method: green carving surface presentation is thallus, protonema, cormus, callus four kinds of states, cultivates without heating outside transfer chamber, temperature 5-25 DEG C, illumination control 0-25000LX, humidity 60-95%;
Second cultivating method: protonema is divided into thallus state, cultivates without heating outside transfer chamber, illumination control 0-4500LX, temperature 5-25 DEG C, humidity 60-90%.
8. according to the preparation method of the moss green carving one of claim 1 to 6 Suo Shu, it is characterized in that, film-forming state in step (5) is main control by the kind of film forming agent and component, described film forming agent is essence of loosening the soil, the not starch-containing composition of described medium, breeding method comprises the first cultivating method and the second cultivating method, and it is with the numerous bud of bud that described first cultivating method grows direction; It is protonema that described second cultivating method grows direction;
Film forming judges feature: liver moss stoste covers green carving skeleton surface completely;
First cultivating method comprises with bud numerous bud inducement phase with bud numerous bud seeding stage:
Principal character in the cultivating process of the first cultivating method: green carving skeleton surface has young moss seedlings to produce;
Second cultivating method comprises protonema induction period, protonema seeding stage;
Principal character in the cultivating process of the second cultivating method: green carving skeleton surface has a large amount of protonema to produce, and produces with a small amount of young moss seedlings;
With bud numerous bud inducement phase: time 20-30 days, temperature 5-28 DEG C, illumination below control 3500LX, humidity 60-90%;
With bud numerous bud seeding stage: temperature 5-28 DEG C, illumination below control 3500LX, humidity 60-90%;
Protonema induction period: time 25-45 days, temperature 18-25 DEG C, illumination control 0-8000LX, humidity 60-90%;
The protonema seeding stage: temperature 5-28 DEG C, illumination below control 3500LX, humidity 60-90%.
9. according to the preparation method of the moss green carving one of claim 1 to 6 Suo Shu, it is characterized in that, the film-forming state in step (5) is main control by the kind of film forming agent, component and starch component, and described growth direction is with the numerous bud of bud; First cultivating method film forming agent essence of loosening the soil, starch component is potato composition; The compound of the second cultivating method film forming agent sodium alginate or sodium alginate and essence of loosening the soil, starch component is tapioca starch;
Film forming judges feature: liver moss stoste covers green carving skeleton surface completely;
, in this breeding method, there are one of following two kinds of features in principal character in cultivating process:
First cultivating method feature: liver moss stoste was transferred to green carving skeleton surface after 1-3 days, be exposed to illumination and assign comprehensively dry to carry out sterilizing, green carving skeleton surface has young moss seedlings to produce;
Second cultivating method feature: without the need to being exposed to sterilizing under illumination after liver moss stoste is transferred to green carving skeleton surface, green carving skeleton surface has young moss seedlings to produce;
Comprise with bud numerous bud inducement phase with bud numerous bud seeding stage with bud numerous bud cultivating process;
One of following two kinds of breeding methods are comprised with bud numerous bud inducement phase:
First cultivating method: liver moss stoste is transferred to green carving skeleton surface 1-3 days, is then exposed to illumination and assigns comprehensively dry to carry out sterilizing, and then soak or spray water and put shady place, cultivate 20-30 days continuously;
Second cultivating method: after liver moss stoste is transferred to green carving skeleton surface, cultivates 20-30 days continuously;
The breeding condition of the first cultivating method and the second cultivating method comprises temperature 5-28 DEG C, illumination below control 3500LX, humidity 60-90%;
With bud numerous bud seeding stage: temperature 5-28 DEG C, illumination below control 3500LX, humidity 60-90%, penetrates in 1 hour, to help growth with 15000-25000LX illumination 1-2 time weekly.
10. according to the preparation method of the moss green carving one of claim 1 to 6 Suo Shu, it is characterized in that, film-forming state in described step (5) is main control by the kind of film forming agent, component and starch component, described film forming agent is the compound of water-loss reducer or water-loss reducer and essence of loosening the soil, described medium contains starch component, and described growth direction is callus;
Film forming judges feature: liver moss stoste covers green carving skeleton surface completely and has reflective; Principal character in cultivating process: after induction period, green carving skeleton surface has callus and bud to produce;
Callus cultivating process comprises the callus induction phase, callus turns protonema and the protonema seeding stage;
The callus induction phase: time 20-30 days, temperature 20-30 DEG C, illumination below control 2500LX, humidity 70-90%; Callus turns the protonema phase: time 25-45 days, temperature 15-25 DEG C, illumination control 3500-15000LX, humidity 60-90%; The protonema seeding stage: temperature 5-28 DEG C, illumination below control 3500LX, humidity 60-90%.
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CN106538274A (en) * 2016-11-25 2017-03-29 上海应用技术大学 A kind of lawn sculpture manufacture method
CN109618854A (en) * 2019-01-25 2019-04-16 福建师范大学福清分校 The method of moss is cultivated on a kind of charcoal
CN114158445A (en) * 2021-11-30 2022-03-11 丽水学院 Light supplementing and maintaining device for moss vegetative propagation and maintaining method thereof

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