CN105009900B - A kind of preparation method of moss green carving - Google Patents

A kind of preparation method of moss green carving Download PDF

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CN105009900B
CN105009900B CN201510431899.7A CN201510431899A CN105009900B CN 105009900 B CN105009900 B CN 105009900B CN 201510431899 A CN201510431899 A CN 201510431899A CN 105009900 B CN105009900 B CN 105009900B
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moss
green carving
breeding
liver moss
cultivating
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CN105009900A (en
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吴军
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吴军
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H11/00Bryophytes, e.g. mosses, liverworts

Abstract

The present invention provides a kind of preparation method of moss green carving, is related to friendly process product field.The preparation method is the application to the open tissue culture technology of liver moss, preliminary treatment is carried out by liver moss raw material, then carry out with culture medium being mixed with liver moss stoste again, it is transferred into again on green carving skeleton, liver moss is cultivated to control the development direction of liver moss by controlling the film-forming state of the liver moss stoste on green carving skeleton surface, all kinds of moss green carving moulding are finally produced.The low production cost of the moss green carving cultivated of moss green carving preparation method that the present invention is provided, moulding are lively, maintenance is convenient, and the free posing for being very suitable for factory's batch production and individual makes.

Description

A kind of preparation method of moss green carving
Technical field
The present invention relates to friendly process product field, in particular to a kind of preparation method of moss green carving.
Background technology
The breeding method of current liver moss moulding is mainly to be cultivated by the way of glue is colonized.First, will be used to cultivate The mould of liver moss moulding such as wooden model surface clean is clean, then, by field planting glue uniform application in die surface, finally, by newly Fresh liver moss be transplanted to scribble field planting glue die surface cultivated, the liver moss moulding cultivated in this way have one Determine aesthetic feeling.However, by the way that liver moss to be transplanted to the method that the die surface of field planting glue is cultivated that scribbles, migration process is very numerous It is trivial, large-scale production is not easy to, therefore the liver moss covering of its liver moss moulding is few.Meanwhile, on wooden model using field planting glue for The effect of liver moss adhesive water and nutriment is limited, and the liver moss moulding needs for causing this mode to be cultivated very frequently are supported Shield, and artificial graft's seedling liver moss can cause its gametophyte and sporinite natural beauty to be greatly lowered.
The content of the invention
In view of this, the purpose of the embodiment of the present invention is to provide a kind of breeding method of green carving, to improve above-mentioned asking Topic.
A kind of preparation method of moss green carving, comprises the following steps:
(1) the liver moss raw material of collection is carried out into preliminary treatment;(2) by the culture medium needed for moss growth and by step (1) Liver moss raw material after treatment mixes liver moss stoste;(3) make green carving skeleton and the liver moss stoste is transferred to by going out The green carving skeleton of bacterium;(4) the green carving skeleton that will be covered with the liver moss stoste is positioned in breeding device;(5) basis is covered in The film-forming state of the liver moss stoste on green carving skeleton surface controls the development direction of liver moss, carries out moss cultivation.
Further, the preliminary treatment in step (1) includes following operation:(1.1) it is former to the liver moss for gathering first Material cleaning decontamination;(1.2) sterilization treatment is carried out to the liver moss raw material gone after the removal of impurity, then liver moss raw material is carried out at water Reason, makes liver moss raw material be in moisture state.
Further, the culture medium in step (2) includes following composition:(2.1) the nutrition unit needed for moss growth Plain mother liquor, plant growth regulator mother liquor, insecticide, anti-browning agent, anti-pollutant, carbohydrate and yeast juice;(2.2) single type Artificial endosperm or mixed type artificial endosperm:Single type artificial endosperm:By film forming agent it is soaked or stirring be made;Mixed type people Work endosperm:By film forming agent it is soaked or stirring, then starch component is mixed with film forming agent.
Further, the preparation liver moss stoste in step (2) includes multi-step mixing facture or synchronous stirring system The practice:Multi-step mixing facture:Each component in step (2.1) is mixed in container, the temperature of liquid is made to being added water in container It is 20 DEG C~45 DEG C, is stirred 1-2 minutes in being then put into mixer together with the liver moss raw material by preliminary treatment;To make again Single type artificial endosperm or mixed type artificial endosperm be put into mixer, be stirred for 1-2 minutes;Adjusted to pH value is added in mixer Section agent obtains liver moss stoste to adjust pH value as neutral or faintly acid;Synchronous stirring facture:By each group in step (2.1) Divide and be mixed in container, the temperature for making liquid to being added water in container is 20 DEG C~45 DEG C, then with the liver moss by preliminary treatment Raw material, single type artificial endosperm or mixed type artificial endosperm are put into mixer, are stirred 0.5-2 minutes;Add in mixer again Enter pH value regulator to adjust pH value as neutral or faintly acid, obtain liver moss stoste.
Further, making green carving skeleton and the liver moss stoste be transferred to the green carving by sterilizing in step (3) Skeleton includes following operation:
(3.1) make green carving skeleton and by the skeleton first soaked cleaning of green carving, be then placed in timing in the sterilized solution of constant volume Sterilizing is sterilized by illumination, and the green carving skeleton includes metal net layer, surface layer and suture;The surface layer is laid in institute State on metal net layer, the metal net layer and the surface layer are sutured by the suture and fixed;
(3.2) green carving skeleton is made to keep moisture state afterwards with the green carving skeleton after clear water drip washing sterilization treatment again;
(3.3) liver moss stoste is transferred in the green carving skeleton of moisture state so that be covered in the tongue on green carving skeleton surface Moss stoste reaches and does not slide, and the method for the transfer includes any one or more in spraying, brushing, dip-coating.
Further, the breeding device in step (4) includes one of following device:
The reflective breeding device of buckle-type:By breeding box, mesh-supported frame, reflex reflector and by light transmissive material make Breeding cover is constituted.The cover top of breeding cover or side top are provided with air-vent, and water is filled in the breeding box, and mesh-supported sets up to be placed in educates Plant in case and away from the water surface, green carving skeleton is positioned on mesh-supported frame, breeding cover buckles the side seam located at breeding box;Reflex reflector It is made up of reflective membrane and reflector, reflective membrane is layed in breeding chamber interior wall and the week side of boss, reflector is outer located at breeding box side;
The reflective breeding device of split:By breeding box, support frame, reflex reflector and the breeding by light transmissive material making Cover is constituted.The cover top of breeding cover or side top are provided with air-vent, fill water in breeding box, support frame be arranged in breeding box and away from The water surface, green carving skeleton is positioned on support frame, and breeding cover is buckled in breeding box and stretched under the water surface.Reflex reflector is by reflective Film and reflector are constituted, and reflective membrane is layed on support frame or is layed in breeding box inner circumferential side, reflector in breeding box or Outside breeding box.
Further, the film-forming state described in step (5) is by based on the species of film forming agent, component and starch component Control, the film forming agent is the compound of water-loss reducer or water-loss reducer and essence of loosening the soil, and the culture medium contains starch component, described The development direction of liver moss is protonema;
Film forming judges feature:Liver moss stoste is completely covered green carving skeleton surface, and with reflective;
Principal character in cultivating process:The viridescent protonema in green carving skeleton surface is produced;
The cultivating process of protonema includes protonema induction period and protonema seeding stage;
Protonema induction period:20-35 days time, except 18-25 DEG C of fine day outer control temperature, intensity of illumination is 0-8000LX, Humidity 75-97%;Within 30 DEG C, can be spaced carries out a fine day illumination, light for one week to temperature at the green carving skeleton of fine day control According to strength control in below 12000LX, continuous sunny need to do shading treatment, cloudy to shelter from heat or light;
The protonema growth seeding stage includes one of following two kinds of breeding methods:
First cultivating method:Green carving surface is rendered as thallus, protonema, cormus, callus, four kinds of states, transfer It is outdoor to be cultivated without heating, 5-25 DEG C of temperature, illumination control 0-25000LX, humidity 60-95%;
Second cultivating method:Protonema is divided into thallus state, and transfer is outdoor to cultivate without heating, illumination control 0- 4500LX, 5-25 DEG C of temperature, humidity 60-90%,.
Further, the species and component that the film-forming state in step (5) passes through film forming agent are main control, the film forming agent It is essence of loosening the soil, the culture medium is free of starch component, breeding method includes the first cultivating method and the second cultivating method, first training It is using buds to propagate buds to educate method development direction;The second cultivating method development direction is protonema;
Film forming judges feature:Liver moss stoste is completely covered green carving skeleton surface;
First cultivating method includes using buds to propagate buds induction period and using buds to propagate buds seeding stage:
Principal character in first cultivating method cultivating process:Green carving skeleton surface has young moss seedlings to produce;
Second cultivating method includes protonema induction period, protonema seeding stage;
Principal character in second cultivating method cultivating process:Green carving skeleton surface has a large amount of protonemas to produce, and with a small amount of Young moss seedlings are produced;
Using buds to propagate buds induction period:20-30 days time, 5-28 DEG C of temperature, illumination control below 3500LX, humidity 60-90%;
The using buds to propagate buds seeding stage:5-28 DEG C of temperature, illumination control below 3500LX, humidity 60-90%;
Protonema induction period:25-45 days time, 18-25 DEG C of temperature, illumination control 0-8000LX, humidity 60-90%;
The protonema seeding stage:5-28 DEG C of temperature, illumination control below 3500LX, humidity 60-90%;
Further, the film-forming state in step (5) is master control by the species of film forming agent, component and starch component System, the development direction is using buds to propagate buds;First cultivating method film forming agent essence of loosening the soil, starch component is potato composition;Second training Educate method film forming agent sodium alginate or sodium alginate and the compound of essence of loosening the soil, starch component is tapioca starch.
Film forming judges feature:Liver moss stoste is completely covered green carving skeleton surface;
, there is one of following two kinds of features in the breeding method in principal character in cultivating process:
First cultivating method feature:Liver moss stoste was transferred to green carving skeleton surface after 1-3 days, was assigned exposed to illumination comprehensively dry It is dry to be sterilized, green carving skeleton surface has young moss seedlings to produce;
Second cultivating method feature:Liver moss stoste sterilizes after being transferred to green carving skeleton surface without exposure under illumination, green carving Skeleton surface has young moss seedlings to produce;
Using buds to propagate buds cultivating process includes using buds to propagate buds induction period and using buds to propagate buds seeding stage;
Using buds to propagate buds induction period includes one of following two kinds of breeding methods:
First cultivating method:Liver moss stoste is transferred to green carving skeleton surface 1-3 days, is then exposed to illumination and assigns comprehensive drying To be sterilized, then soak again or spray water and put shady place, it is continuous to cultivate 20-30 days;
Second cultivating method:It is continuous to cultivate 20-30 days after liver moss stoste is transferred to green carving skeleton surface;
The breeding condition of the first cultivating method and the second cultivating method includes 5-28 DEG C of temperature, and illumination controls below 3500LX, wet Degree 60-90%;
The using buds to propagate buds seeding stage:5-28 DEG C of temperature, illumination control below 3500LX, humidity 60-90%, weekly 1-2 times with In 15000-25000LX light irradiations 1 hour, to help growth.
Further, the film-forming state described in step (5) is by based on the species of film forming agent, component and starch component Control, the film forming agent is the compound of water-loss reducer or water-loss reducer and essence of loosening the soil, and the culture medium contains starch component, described Development direction is callus;
Film forming judges feature:Liver moss stoste is completely covered green carving skeleton surface and with reflective;It is main in cultivating process Feature:Green carving skeleton surface has callus and bud to produce after induction period;
Callus cultivating process includes that callus induction phase, callus turn protonema and protonema seeding stage; The callus induction phase:20-30 days time, 20-30 DEG C of temperature, illumination control below 2500LX, humidity 70-90%;
Callus turns the protonema phase:25-45 days time, 15-25 DEG C of temperature, illumination control 3500-15000LX, humidity 60-90%;The protonema seeding stage:5-28 DEG C of temperature, illumination control below 3500LX, humidity 60-90%.
The moss green carving preparation method that the present invention is provided is by controlling the film-forming state of the liver moss stoste on green carving skeleton surface Moss cultivation is carried out to control the development direction of liver moss, so as to make moss green carving.Liver moss stoste is on green carving skeleton surface Film-forming state directly affects the development direction of liver moss so that the cultivation of liver moss has more preferable controllability.
The characteristics of moss green carving that this method makes has clean, attractive in appearance, and moulding is lively, due to can neatly select Select breeding method, thus be applicable to it is all view and admire liver moss, and be effectively reduced in moss green carving manufacturing process when cultivating liver moss The frequency and difficulty of maintenance work, also improve disease-resistant, the worm ability for cultivating liver moss in addition.This preparation method is simple, be easy to operation, Its production can be substantially reduced and rise this, be very suitable for large-scale production.
To enable the above objects, features and advantages of the present invention to become apparent, preferred embodiment cited below particularly, and coordinate Appended accompanying drawing, is described in detail below.
Brief description of the drawings
In order to illustrate more clearly of the technical scheme of new embodiment of the present invention, below will be to be used needed for embodiment Accompanying drawing be briefly described, it will be appreciated that the following drawings illustrate only certain embodiments of the present invention, therefore be not to be seen as It is the restriction to scope, for those of ordinary skill in the art, on the premise of not paying creative work, can be with root Other related accompanying drawings are obtained according to these accompanying drawings.
Fig. 1 is the flow chart of the preparation method of the moss green carving that the embodiment of the present invention one is provided;
The structural representation of the green carving skeleton for using in the preparation method of the moss green carving that Fig. 2 is provided for Fig. 1.
Description of reference numerals
Stratum reticulare 101;Surface layer 102;Suture 103.
Specific embodiment
Below in conjunction with accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete Ground description, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.Generally exist The component of the embodiment of the present invention described and illustrated in accompanying drawing can be arranged and designed with a variety of configurations herein.Cause This, the detailed description of the embodiments of the invention to providing in the accompanying drawings is not intended to limit claimed invention below Scope, but it is merely representative of selected embodiment of the invention.Based on embodiments of the invention, those skilled in the art are not doing The every other embodiment obtained on the premise of going out creative work, belongs to the scope of protection of the invention.
Embodiment one
Refering to Fig. 1, a kind of preparation method of moss green carving is present embodiments provided, the preparation method comprises the following steps:
(1) the liver moss raw material of collection is carried out into preliminary treatment, liver moss raw material can be broken for the gametophyte of various liver mosses, liver moss Duan Ganmo etc..(2) culture medium needed for moss growth is mixed into liver moss original with the liver moss raw material after being processed by step (1) Liquid.(3) make green carving skeleton and the liver moss stoste is transferred to the green carving skeleton by sterilizing.(4) will be covered with the tongue The green carving skeleton of moss stoste is positioned in breeding device.(5) according to the film-like of the liver moss stoste for being covered in green carving skeleton surface State carries out moss cultivation come the development direction for controlling liver moss.
Wherein, the film-forming state of liver moss stoste refer to liver moss stoste be covered in it is green carving skeleton surface after, liver moss stoste Attachment state, for example, in example by by water-loss reducer it is soaked after again high speed 25000r/m stir, make water-loss reducer from coarse become Gluey into thicker (water-loss reducer consumption is lower slightly) or thick (water-loss reducer consumption is higher), gel strength is high, such as water board water-loss reducer In introduction:Due to introducing rigid inorganic raw material in product, water retention is more than after 3000r/m is centrifuged 30 minutes in the centrifuge 93%, 250g~500g/cm2 can be withstood forces;Institute is more sticky into liver moss stoste after mixing, current to be transferred to green carving skeleton Behind surface, liver moss raw material is subject to the pressure ratio of liquid larger, and the pressure that moss growth is emerged is also big, and increasing starch constituents can enter One step increases its pressure;For example with water adhesion agent loosen the soil essence it is soaked after, resynchronisation stirring formed liver moss stoste mobility It is good or when having certain foam, it is current be transferred to green carving skeleton surface after, adhesive shaping or when forming certain alveolate texture, tongue Moss raw material is smaller by the pressure of liquid, and the pressure that moss growth is emerged is also small.The growth course of liver moss is experienced certainly with liver moss The pressure for arriving is relevant, and film-forming state of the liver moss stoste on green carving skeleton surface can then play crucial effect to the pressure of liver moss, So being covered in the film-forming state of the liver moss stoste on green carving skeleton surface and coordinating the environmental Kuznets Curves to directly influence the hair of liver moss Educate direction.The film-forming state of liver moss stoste and the species of film forming agent and component, the presence or absence of starch material, culture medium it is thick The factors such as degree, the broken segment length of liver moss, transfer operation are relevant.For example, film-forming state is by aqueous control:Control culture medium reclaimed water Content;Film-forming state is controlled by the amount of liver moss raw material:Control volume and/or the quality control of liver moss raw material;Film-forming state Controlled by the broken segment length of liver moss raw material:Different growing environment according to liver moss controls broken section of length;Film-forming state passes through The specific method control of transfer operation.For example when liver moss stoste is diluter, or broken section it is long or formation be uneven on green carving surface When can not form reflective, the pressure that part liver moss is subject to is small, in the winter time the induction of precursor body method or the induction of callus method When, still occur that part liver moss seedling occurs, then can not form Cultivated objective uniformity.
Preferably, liver moss stoste is completely covered green carving skeleton surface, and liver moss stoste can not slide.Film forming agent includes three Kind:The compound of gel class, water adhesion agent class, gel-like and water adhesion agent.The film forming agent of gel class such as water-loss reducer etc. Film pressure is big, so as to the growth pressure to liver moss is larger, further, can be added in gel class film forming agent starch with Increase film pressure.The film forming agent of water adhesion agent class such as loosen the soil essence or konjac glucomannan film pressure it is small, therefore to the life of liver moss Pressure long is also relatively small.
Due in the growth and development process of liver moss part bacterium can consume moss growth needed for various nutritional ingredients, together When also discharge the material poisonous to liver moss, so as to influence the normal growth of liver moss.Therefore, it can tentatively locate liver moss raw material Manage to reduce its bacterium, debris for carrying etc..First, the liver moss raw material for gathering is cleaned, in removal liver moss raw material Impurity, such as wood chip, sandstone, rotted leaf etc..Secondly, the liver moss raw material after decontamination is carried out into sterilization treatment, for example, using clear water Cleaned, cleaned using the washing powder with bactericidal action.Again, water process is carried out to liver moss raw material, makes tongue Moss raw material keeps moisture state, it is therefore an objective to beneficial to control liver moss stoste water content, while absorbing nutritional ingredient beneficial to liver moss.Moistening State is the state of the whole surface that liquid film is formed at liver moss raw material, for example, broken section of liver moss is covered by liquid film.It is preferred that Sterilization treatment can also be carried out to green carving skeleton, it is to avoid be attached to influence of the bacterium on green carving skeleton to moss growth.For example, Sterilization treatment can be carried out to green carving skeleton using high-temperature sterilization, ultraviolet sterilization etc. mode.Additionally, being carried out to liver moss raw material Be should be noted that when cleaning, sterilization treatment and avoid that it is caused to damage, and influence the normal growth of liver moss raw material.
After the completion of to liver moss raw material preliminary treatment, then liver moss stoste is made, the liver moss stoste is included needed for moss growth Culture medium and the liver moss raw material by preliminary treatment.Culture medium provides various nutrients, growing environment for the growth of liver moss And other promote the composition of growth and suppression pest and disease damage.Culture medium includes following components in the present embodiment:Needed for moss growth Nutrient mother liquor, plant growth regulator mother liquor, insecticide, anti-browning agent, antipollution, carbohydrate, yeast juice and artificial Endosperm, wherein artificial endosperm include two kinds, respectively single type artificial endosperm and mixed type artificial endosperm.Wherein, single type people Work endosperm by by film forming agent it is soaked or stirring be made;Mixed type artificial endosperm by by film forming agent it is soaked or stirring, so Mix with starch component again afterwards and be made.
Culture medium can then carry out the making of liver moss stoste after completing, preparing liver moss stoste includes multi-step mixing facture With two methods of synchronous stirring facture.
Wherein, multi-step mixing facture:First, adjusted to the nutrient mother liquor needed for above-mentioned moss growth, plant growth Section agent mother liquor, insecticide, anti-browning agent, antipollution, carbohydrate, yeast juice are mixed in a container, and make to being added water in the container The temperature of liquid is 20 DEG C~45 DEG C.Next, stirs 1-2 minutes with being put into mixer by the liver moss raw material of preliminary treatment, Preferably 1 minute, required and time saving beneficial to controlling broken segment length to reach.Again, by single type artificial endosperm or the artificial embryo of mixed type Breast is stirred 1~2 minute in adding mixer.Finally, in mixer add pH value regulator with adjust pH value for neutrality or Faintly acid, obtains liver moss stoste, and pH value regulator can use sodium hydrate aqueous solution or hydrochloride aqueous solution.
Synchronous stirring facture:First, it is female to the nutrient mother liquor needed for above-mentioned moss growth, plant growth regulator Liquid, insecticide, anti-browning agent, antipollution, carbohydrate, yeast juice are mixed in a container, and make liquid to being added water in the container Temperature is 20 DEG C~45 DEG C.Secondly, with the liver moss raw material by preliminary treatment, single type artificial endosperm or mixed type artificial endosperm It is put into mixer, stirs 0.5-2 minutes, preferably 0.5 minute, is required and time saving beneficial to controlling broken segment length to reach;Again to stirring Addition pH value regulator obtains liver moss stoste to adjust pH value as neutral or faintly acid in mixing machine.PH value regulator can be adopted With sodium hydrate aqueous solution or hydrochloride aqueous solution, the pH value of liver moss stoste is preferably 6.5≤PH≤7, such as 6.5,6.6,6.8 Or 7.
After liver moss stoste completes, then green carving skeleton surface can be transferred them to, detailed process is as follows:Step 1:It is first First, by the skeleton first soaked cleaning of green carving, to remove green spot for carving skeleton surface etc..Then, green carving skeleton is carried out at sterilizing Reason, it is to avoid development of the green carving skeleton Carried bacteria to liver moss is interfered.Specifically, it is possible to use the sterilized solution of constant volume is as first used Washing powder, then be timed with such as bromogeramine liquid or other sterilized pharmaceutics, quantitatively sterilized, or sterilized by illumination.Step 2:The green carving skeleton clear water drip washing that will be processed by step 1, makes green carving skeleton keep micro- wet, it is for instance possible to use dry Mode, removes the excessive moisture on green carving skeleton surface.Because green carving skeleton surface is in moisture state, so as to be conducive to liver moss The attachment and distribution of stoste;When being sterilized using illumination to green carving skeleton, green carving skeleton can be made directly to keep dry state.Step 3:Liver moss stoste is transferred to the green carving skeleton in moistening or drying regime so that be covered in the liver moss stoste on green carving skeleton surface Do not slide.When the liver moss stoste on green carving skeleton surface is excessive, then the density for easily causing liver moss raw material is excessive, so as to cause liver moss Being competed in growth course influences greatly its normal growth, and the liver moss stoste for sliding may pollute breeding device, and influences tongue The growing environment of moss, it is therefore preferred that the liver moss stoste for being transferred to green carving skeleton surface is not reunited.The method bag of transfer Include spraying or brush or dip-coating, specific transfer method is not specifically limited in the present embodiment.
Green carving skeleton is the substrate of moss growth, and the shape of green carving skeleton limits the shape of final green carving skeleton.According to Actually used scene needs, and the shape of green carving skeleton can have various, and carcasing into all kinds of shapes is carved by green using method for folding Shape, for example, various animal shapes or various potted landscape profiles etc..The material of green carving skeleton can use wire netting, preferably aluminium Alloy network, aluminium alloy net soft texture is easy to shape and with preferable resistance to corrosion, light quality, moss green beneficial to the later stage The movement of carving.Green carving skeleton can also use fiber-like material, preferably scouring pad, and its surface is relatively rougher, and it has very Many micropores, on the premise of gas permeability is ensured, moreover it is possible to improve the attachment stability of liver moss stoste.Certainly green carving skeleton can be used for nothing Soil can also be used for earth culture and educate moss green carving, it may also be used for the green carving of Tom Thumb ornamental plant.
After liver moss stoste is transferred to green carving skeleton, then green carving skeleton can be positioned in breeding device, carry out the training of liver moss Educate.Present embodiments provide that buckle-type is reflective and the reflective two kinds of breeding devices of split, two can be selected according to actual demand Breeding device or part thereof composition is planted, breeding device is not limited to two kinds of the present embodiment offer.
Wherein, the breeding cover that the reflective breeding device of buckle-type makes by breeding box, mesh-supported frame and by light transmissive material Constitute.The cover top of breeding cover or side top are provided with air-vent, water are filled in breeding box, it is preferable that dry season is contained in breeding box There is water, be not filled with water in overcast and rainy season,.Bottom in breeding box, side wall are preferably white or are directly with reflective membrane function, mesh-supported frame It is arranged in the breeding box and away from the water surface.Green carving skeleton is positioned on the mesh-supported frame, and breeding cover is buckled located at described The side seam of breeding box.
Preferably, seedlings nursing plate and reflector, the reflective breeding dress of buckle-type can also be set up to the reflective breeding device of buckle-type Storing is placed in seedlings nursing plate, reflector be arranged in seedlings nursing plate or it is neighbouring be arranged at outside breeding box, and it is neighbouring to be bent to arc-shaped The reflective breeding device of buckle-type.The reflecting angle of reflector can adjust, it is preferred that the reflecting angle of reflector is controlled to 45~90 ℃.To increase the reflecting effect of reflector, reflective membrane can be set towards the side of buckle-type reflective breeding device in reflector.Enter One step ground, can also improve multi-angle reflecting effect, when filling water in breeding box in the interior bottom of breeding box and inwall lay reflex film When, reflective membrane can be also layed on support frame.Trying to get to the heart of a matter for seedlings nursing plate can as the case may be set through hole, be used to discharge and educate The water of savings in seed plate.
The reflective breeding device of split is constituted by breeding box, support frame and by the breeding cover that light transmissive material makes.Breeding The cover top of cover or side top are provided with air-vent, and water is filled in breeding box, and support frame is arranged in breeding box and away from the water surface.Green carving bone Frame is positioned on institute's mesh-supported frame, and breeding cover is buckled in breeding box and stretched under the water surface.
Preferably, reflector can also be set up to the reflective breeding device of split, reflector is arranged in breeding box or adjacent Closely it is arranged at outside breeding box, and is bent to arc-shaped adjacent to breeding cover.The reflecting angle of reflector can adjust, it is preferred that reflective The reflecting angle of plate is controlled to 45~90 DEG C.To increase the reflecting effect of reflector, can be educated towards buckle-type is reflective in reflector The side for planting device sets reflective membrane.Further, can also on breeding box support frame and breeding chamber interior wall, the week side of boss laying it is reflective Film, improves multi-angle reflecting effect.
Certain buckle-type and the reflective breeding device of split can be not only used for moss green carving and cultivate, it can also be used to which its class plant is trained Educate.
Because green carving skeleton is away from the water surface, when the water evaporation in breeding box, the water of evaporation can be carried out to liver moss stoste Moisturizing, promotes the growth of liver moss raw material.Additionally, the evaporation of water may also operate as adjusting the effect of temperature in breeding device.Preferably Ground, winter can also set up filter with the reflective breeding device of buckle-type and the reflective breeding device of split, and such as hundred is clean Cloth, scouring pad is layed on mesh-supported frame or mesh-supported frame, and the green carving skeleton for being coated with liver moss stoste is positioned over hundred On clean cloth.When the evaporated quantity of water in breeding box is larger, then can be filtered using scouring pad, be prevented the too high cause of vapour-liquid temperature The depauperation of liver moss raw material or liver moss raw material slide but the growth of liver moss is uneven.Preferably, breeding device is positioned over letter In easy greenhouse, for example, vinyl house, to control the environment temperature in breeding device.When external environment temperature than it is relatively low when, In such as winter, heater can be set in breeding device, to promote the growth of liver moss raw material;When the temperature ratio of external environment When higher, such as summer, then heater need not be set, and the reflective breeding device of split can be used to add in breeding device Big reservoir storage and reach cooling-down effect.When the humidity of external environment is higher, in such as summer in overcast and rainy more June, breeding device can Reduction is filled with water or anhydrous, to control liver moss up to the moisture of suitable growth.Due to placing breeding dress under simple greenhouse or tree shade Put three face daylightings strong, simultaneously daylighting is weak, therefore can also use split or the reflective breeding device of buckle-type, buckle-type in simple greenhouse Reflective breeding device is because that need not consider rain proofness, therefore it can be top drilling that breeding is put, and seedlings nursing plate available disk bottom is non-porous or has Kong Yu Seed plate;Need to consider rain proofness under outdoor tree shade, therefore breeding flat-top or dome or other type shape overhead guards are changed to side roof part perforate, rain Water can flow down without the green carving skeleton for damaging breeding naturally, and seedlings nursing plate has sieve aperture disk using bottom, and it is anti-to reach buckle-type with this Light breeding device rain-proof and anti-disk internal water accumulation are acted on.
Heretofore described film forming agent is essence of loosening the soil, and the culture medium is free of starch component, and breeding method includes the first training Method and the second cultivating method are educated, the first cultivating method development direction is using buds to propagate buds;The second cultivating method development direction is original Filament;Split or the reflective breeding device of buckle-type are used again, because easy to operate and be difficult endophytic bacteria and insect pest;Additionally, passing through Split or the reflective breeding device of buckle-type need not exchange light angle up to easily maintenance, multi-angle growth result, therefore be green carving The actual production best approach, therefore request is preferred breeding method.Buckle-type is reflective and reflective two kinds of split is educated in the present invention In kind of device, bottom or side are preferably white or are directly that then bottom or side need not in breeding box with reflective membrane function in breeding box Reflective membrane is repaved, reflective membrane also can use the reflective mirror with reflective membrane function to substitute.Reclaimed water adhesive of the present invention can be with without heating Konjac glucomannan, xanthans, carragheen or its built agent or other play similar action film forming agent as the slightly poor replacement of effect.This hair Can be by similar action film forming agent other or starchy material as the slightly poor replacement of effect in bright middle mixing artificial endosperm;Water-loss reducer Can with having water-loss reducer, dilation is long-lasting repeatedly and stored nutrient, and can directly be dissolved in water and strong without the tool rigidity of stirring The gel of degree more preferably replaces.
Optimization Steps of the present invention and scheme order are as follows:Step 1 is carried out the liver moss raw material of collection in preliminary treatment, liver moss Raw material can also put 4 DEG C of refrigerating chambers and make low-temperature treatment, and liver moss raw material can also be at shade using peat growing medium breeding or river sand nutrient solution Breeding, so as to obtain activity more preferably gametophyte, when sterilization treatment adds bactericidal agent in the medium, only can make simple with washing powder Sterilization treatment, sterilizes again without sterile medicals such as bromogeramines, plays saving working hour water-saving effect.Only water adhesion agent is used in the present invention Without culture medium or culture medium the poor moss green carving of effect can be made without carbohydrate as liver moss fluid sowing.In the present invention Culture medium, to expand numerous substantially, can make the slightly poor green carving of effect containing only carbohydrate and big moderate-element.Add suitable for plant growth regulating Agent can expand numerous yarn number, and the liver moss for example having only can expand numerous 16 with white sugar, and it is 18.5 to add 2,4-D to expand numerous yarn number, concentration Can be in 0.1-1mg/L;Addition cytokinin such as 6-BA concentration can be in 0.25-8mg/L;A- Nafusakus are made auxin and are resisted Hot dry wind is acted on, and 3- butyric acid potassiums make rush rooting effect;Add starch such as can play depot fertilizer, delay without filtering potato grain, juice Punching, water suction, loose permeation functions;Angel Yeast can sharp moss growth and the starch constituents that become thoroughly decomposed effect;Ascorbic acid rises anti-brown Change is acted on;Activated carbon or diatomite play antipollution, slow releasing function;First just play bactericide up to bright shield;First just reach sharp victory or first Shell amine plays insecticide, and clean water quality without insecticide or can change into spray in cultivating process and suit the medicine to the illness insecticide;Diethyl aminoethyl hexanoate is multiple Nitre has confusingly been received and has aided in differentiation profit growth raising resistance to act on.Preferably by controlling illumination, temperature, the humidity of environment in the present invention Liver moss is cultivated etc. factor.It is suitable for indoors constant temperature, constant humidity, permanent light or alternating temperature, get wet, individual layer under the conditions of darkening Or carry out tissue cultures liver moss on multi-layer rack and make moss green carving, equally can also be according to different liver moss kinds from the present invention In the environmental condition of offer or culture medium again suboptimization with up to optimal moss growth condition.Breeding method of the present invention is also applied for it It is adapted to the green carving skeleton of moss growth.Skeleton of the present invention and breeding device and breeding method are equally applicable to DIY client voluntarily Cultivate green carving.The green carving liver moss density of using buds to propagate buds is lower slightly in breeding method of the present invention, and the time is short, and production is easy;Protonema is induced Green carving liver moss density is high, and step is more, and environmental Kuznets Curves requirement is higher, and the time is long, and production difficulty is higher.
Embodiment two
The present embodiment provides a kind of preparation method the method for moss green carving and comprises the following steps:
The first step, preliminary treatment is carried out by the liver moss raw material of collection:First, collection moss uprightly grow thickly make represent it is many The gametophyte of capsule song tail moss, is then cleaned with water to remove the impurity such as soil, rubble, withered grass leaf, is then entered with washing powder and water Row cleaning.Secondly, the gametophyte sterilization treatment of many capsules song tail moss after using bromogeramine reagent to cleaning, afterwards will be new with water Geramine is rinsed well, and finally extracting the water in liver moss raw material makes the gametophyte of many capsule song tail moss in moisture state.
Second step, liver moss is mixed by the culture medium needed for moss growth with the liver moss raw material after being processed by step (1) Stoste:
The first culture medium include moss growth needed for nutrient mother liquor, plant growth regulator mother liquor, insecticide, BPH resistant rice variety and anti-pollutant, carbohydrate, yeast juice and mixed type artificial endosperm.Specifically, the nutrient needed for moss growth Mother liquor:Prepared with KNOP, potassium nitrate 250mg/L, potassium dihydrogen phosphate 250mg/L, calcium nitrate 1000mg/L, magnesium sulfate 250mg/L, zinc sulfate 3mg/L.Plant growth regulator mother liquor is prepared with following ratio:6-BA1~8mg/L 0.1mol NaOH warm water hydrotropy solutions, a- Nafusakus 0.5-2mg/L warm water hydrotropy solutions, 3- butyric acid potassiums 0.25-1mg/L is helped with warm water Dissolving, DA-6 diethyl aminoethyl hexanoate 1-8mg/L warm water hydrotropy solutions.Insecticide is first just up to sharp victory 1:10000 (pesticide-seeds ratios), carbohydrate such as white sugar 30g/L, Angel Yeast 0.32g/L, ascorbic acid 2mg/L, activated carbon 0.7g/L, to play anti-in the growth course of liver moss Brown stain, antipollution, the effect of sustained release agent.Mixed type artificial endosperm:By film forming agent it is soaked or stirring make, then by murphy juice into Divide and mixed with film forming agent, film forming agent uses the water-loss reducer of 7ml, it is preferable that film forming agent is loosened the soil using 9 one-tenth water-loss reducers and 1 one-tenth The mixture of essence.
Second culture medium include moss growth needed for nutrient mother liquor, plant growth regulator mother liquor, insecticide, Anti-browning agent, anti-pollutant, carbohydrate, yeast juice and mixed type artificial endosperm.Nutrient mother liquor needed for moss growth:With KNOP is prepared, potassium nitrate 250mg/L, potassium dihydrogen phosphate 250mg/L, calcium nitrate 1000mg/L, magnesium sulfate 250mg/ L, zinc sulfate 3mg/L.Plant growth regulator mother liquor is prepared with following ratio:2,4-D 0.1moL NaOH warm water hydrotropies The concentration of solution and 2,4-D is 0.5-1mg/L, DA-6 diethyl aminoethyl hexanoate 0.5-3mg/L warm water hydrotropy solutions.Insecticide is first just up to sharp victory 1: 10000 (pesticide-seeds ratios), carbohydrate such as white sugar 30g/L, Angel Yeast 0.32g/L, ascorbic acid 2mg/L, 0.7 gram/L of activated carbon are used To play a part of BPH resistant rice variety, antipollution, sustained release agent in the growth course of liver moss.Film forming agent uses 3.5ml water-loss reducers, preferably Ground, film forming agent using 9 one-tenth water-loss reducers and 1 one-tenth loosen the soil essence mixture.
Prepare liver moss stoste:
First, needed for above-mentioned moss growth nutrient mother liquor, plant growth regulator mother liquor, insecticide, BPH resistant rice variety Agent, antipollution, carbohydrate, yeast juice are mixed in a container, and the temperature for making liquid to being added water in the container is 35 DEG C~45 DEG C, Secondly, stirred 1-2 minutes in mixer together with the liver moss raw material by preliminary treatment, then put mixed type artificial endosperm Enter in mixer and stir 1-2 minutes.The broken segment length of the gametophyte of the preferred bent tail moss of many modelings is 0.1-0.2cm.Again, to stirring Addition pH value regulator obtains liver moss stoste to adjust pH value as neutral or faintly acid in mixing machine.
3rd step, the liver moss stoste of preparation is transferred to the green carving skeleton of sterilizing.
First, after by the skeleton first soaked cleaning of green carving, timing sterilization or use sunlight photograph in the sterilized solution of constant volume are put into Capable sterilizing is injected, green carving skeleton can keep dry state after illumination sterilizing, be more beneficial for carrying out the transfer operation of liver moss stoste.Its It is secondary, then green carving skeleton is cleaned with clear water, green carving skeleton is kept moisture state.Finally, liver moss stoste is brushed to green carving skeleton On, it is preferred that so that the liver moss stoste brushed in green carving skeleton surface reaches the state not slid.In the present embodiment, transfer side Method, can be using any one or more mode in spraying, brushing, dip-coating during practical operation using brushing.
4th step, the green carving skeleton that will be covered with the liver moss stoste is positioned in breeding device.
In the present embodiment, the breeding that induction period breeding device makes by breeding box, mesh-supported frame and by light transmissive material Cover, siron board constant temperature electric heater are constituted, and the cover top of the breeding cover is provided with air-vent, water is filled in the breeding box, described Mesh-supported is set up and is placed in the breeding box and away from the water surface, and the green carving skeleton is positioned on the mesh-supported frame, institute State breeding cover and buckle and buckle seam, the siron board batch (-type) constant temperature electric heater or similar action heater located at the breeding box side It is positioned in the middle of breeding box bottom, regulation temperature is water in 31 degree of heater boxes, is popped one's head in humiture at moulding and survey the cloudy or cloudy day It is 20-25 degree.Breeding apparatus are in Simple transparent film canopy.
5th step, the development direction of liver moss is controlled according to the film-forming state for being covered in the green liver moss stoste for carving skeleton surface Carry out moss cultivation.
The film-forming state of the liver moss stoste on green carving skeleton surface is by based on the species of film forming agent, component and starch component Control, the film forming agent is the compound of water-loss reducer or water-loss reducer and essence of loosening the soil, and the culture medium contains starch component, described The development direction of liver moss is protonema.The film forming of green carving skeleton surface liver moss stoste judges feature:Liver moss stoste is completely covered green Carving skeleton surface, and with reflective.In this example, there is film forming reflective then can prevent germ contamination in cultivating process, and Certain condensed water can be prevented, and liver moss stoste can be made not slid in cultivation, preserve nutrition, while because institute's controlling temperature is suitable former 20-25 DEG C of mycelial growth, film forming has reflective, and then film forming parcel liver moss seed is tight, and liver moss bud cannot get out, and promote tongue Moss is towards raw water pretreatment;This example film forming agent is played beneficial to stirring into gelatification using compound, and water-loss reducer is not in making stirring Can be flashed machine perisporium, and cleaning robot is easier;Water-loss reducer film can be broken through after raw water pretreatment and is presented on green carving surface.
Principal character in cultivating process:The viridescent protonema in green carving skeleton surface is produced.The cultivating process bag of protonema Include protonema induction period and protonema seeding stage.
Protonema induction period:20-35 days time, except 18-25 DEG C of fine day outer control temperature, preferably 18-22 DEG C, illumination is strong It is 0-8000LX to spend, humidity 75-97%.Within 30 DEG C, can be spaced one week carries out one to temperature at the green carving skeleton of fine day control Secondary fine day illumination, intensity of illumination is controlled in below 12000LX, and continuous sunny need to do shading treatment, cloudy to shelter from heat or light.
The protonema growth seeding stage includes one of following two kinds of breeding methods:First cultivating method:Green carving surface is rendered as leaf Shape body, protonema, cormus, four kinds of states of callus.Film forming agent large usage quantity in mixed type artificial endosperm, by breeding device It is transferred to outdoor without heating cultivation, 5-25 DEG C of temperature, illumination control 0-25000LX, humidity 60-95%, wherein thallus, precursor The nurturing an environment humidity of body state is preferably 70-80%, and temperature is preferably 15-25 DEG C, and the Differentiation Types are cultivated using the first Base.Green carving skeleton is transferred in the reflective breeding device of buckle-type in this type, with the natural enhancing of spring illumination, is picked up shade and is controlled to 0-15000LX, it is thallus and protonema coexistence phase that green carving can be divided into naturally, shade can be picked up after this stage and is controlled to 0-3500LX Help and emerge;It is another by induction period after green carving be transferred in the reflective breeding device of split, and support frame with water with 10 centimetres high green carvings Skeleton bottom is level, can have it is certain pass water function, illumination control 0-35000LX, green carving can naturally be divided into cormus, lobate Body, callus, four kinds of states of protonema, four kinds of states co-exist actual productions it is general without.It is temperature callus reason occur Height is spent, callus is switched to by protonema again.Can also there is another variation characteristic in this example:Because of illumination too low cause induction period Green carving surface redgreen protonema is produced afterwards, can still be presented after being equally transferred to the outdoor breeding device equivalent environment without heating Four kinds of states.Second cultivating method:Protonema is divided into thallus state, in mixed type artificial endosperm film forming agent consumption compared with It is small, the reflective breeding device of buckle-type is transferred to outdoor without heating cultivation, illumination control 0-4500LX, the promotion of 25-45 days time The further development growth of protonema, and beneficial to water spray number of times is reduced, illumination afterwards controls 0-3500LX, preferably 1200- 2500LX.5-25 DEG C of protonema growth seeding stage temperature, preferably 18-22 degree, humidity 60-90% are preferably 70-80%, this point Change type and use second culture medium.Second cultivating method emerge it is denser, maintenance water spray number of times it is less.
This example favorable season in the natural environment is breeding from December in winter.Can not be supplemented in this example cultivating process Nutritional ingredient.Pigment can be also added in culture medium in this example, is used to change liver moss color.Artificial endosperm can in this example Slightly poor replacement is made by sodium alginate plus tapioca starch.By this example water control humiture principle, therefore in batch production, can also train Educating device can be constituted with insulation breeding tank long, support frame, reflector, reflective membrane, large-scale heater, breeding cover.Breeding cover Depending on size is profit purchase or to make or operate, large-scale heater heats water and temperature sizing number, reflective membrane according to needed for And depending on reflective board size radian is with breeding cover.
Embodiment three
The present embodiment provides a kind of preparation method the method for moss green carving and comprises the following steps:
The first step, preliminary treatment is carried out by the liver moss raw material of collection:First, the first cultivating method collection moss is crawled and lies prostrate piece life The gametophyte of the blue or green moss that work is represented;Second cultivating method collection moss is uprightly grown thickly the gametophyte of white hair moss for making to represent;Then Cleaned with water to remove the impurity such as soil, rubble, withered grass leaf, then cleaned with washing powder and water.Finally by liver moss raw material In water extract make liver moss gametophyte be in moisture state.
Second step, liver moss is mixed by the culture medium needed for moss growth with the liver moss raw material after being processed by step (1) Stoste:
Culture medium:First cultivating method culture medium includes nutrient mother liquor, the plant growth regulator needed for moss growth Mother liquor, bactericidal agent, insecticide, anti-browning agent, anti-pollutant, carbohydrate, yeast juice and single type artificial endosperm.Specifically, tongue Nutrient mother liquor needed for moss growth:Prepared with KNOP, potassium nitrate 250mg/L, potassium dihydrogen phosphate 250mg/L, Calcium nitrate 1000mg/L, magnesium sulfate 250mg/L, zinc sulfate 3mg/L.Plant growth regulator mother liquor is matched somebody with somebody with following ratio System:2,4-D is 0.25mg/L, 6-BA0.25mg/L 0.1mol with 0.1moLNaOH warm water hydrotropies solution, the concentration of 2,4-D NaOH warm water hydrotropy solutions, the confused sodium 1.0-1.5mg/L warm water hydrotropy solution of multiple nitre.Liver moss can be induced using the 2,4-D of 0.25mg/L Raw material is produced without callus, and green carving surface has bud to produce;Bactericide first just reaches bright shield 1:1200 (pesticide-seeds ratios), insecticide is first just Up to sharp victory 1:12000 (pesticide-seeds ratios), carbohydrate such as white sugar 12g/L, ascorbic acid 2mg/L BPH resistant rice varieties, Angel Yeast 0.32g/L is living Property charcoal 0.7g/L be used as antipollution, the sustained release agent of blue or green moss.Single type artificial endosperm is made by by film forming agent is soaked, film forming Agent essence of loosening the soil.
Second cultivating method culture medium include moss growth needed for nutrient mother liquor, plant growth regulator mother liquor, go out Microbial inoculum, insecticide, anti-browning agent, anti-pollutant, carbohydrate, yeast juice and single type artificial endosperm.Specifically, moss growth institute The nutrient mother liquor for needing:Prepared with KNOP, potassium nitrate 250mg/L, potassium dihydrogen phosphate 250mg/L, calcium nitrate 1000mg/L, magnesium sulfate 250mg/L, zinc sulfate 3mg/L.Plant growth regulator mother liquor is prepared with following ratio:2,4-D With 0.1moLNaOH warm water hydrotropy solutions, 2,4-D concentration is 0.5mg/L, and bactericide first just reaches bright shield 1:1200 (pesticide-seeds ratios), kill Worm agent is first just up to sharp victory 1:12000 (pesticide-seeds ratios), carbohydrate such as white sugar 30g/L, ascorbic acid 2mg/L BPH resistant rice varieties, Angel Yeast 0.32g/L, diatomite 0.7g/L are used as antipollution, the sustained release agent of white hair moss.6-BA0.25mg/L is helped with 0.1mol NaOH warm water Dissolving, the confused sodium 1-1.5mg/L warm water hydrotropy solution of multiple nitre.Single type artificial endosperm is made by by film forming agent is soaked, film forming Agent essence of loosening the soil.
The cultivating method of the present embodiment first and the second cultivating method culture medium are for moss kind factory formula, because liver moss kind is numerous Therefore each constituent content in culture medium can as the case may be increased or be reduced, not to each component in culture medium Usage ratio is construed as limiting, but should at least contain above-mentioned KNOP nutrients, plant growth regulator, carbohydrate, film forming agent.
Prepare liver moss stoste:First, needed for above-mentioned moss growth nutrient mother liquor, plant growth regulator mother liquor, Bactericide, insecticide, anti-browning agent, anti-pollutant, carbohydrate and yeast juice are mixed in mixer.Secondly, and to the stirring Adding blue or green moss, water in machine makes the temperature of liquid be 25 DEG C, again, is cooperatively stirred to single type artificial endosperm is added in mixer 0.5-1 minutes, it is preferable that the broken segment length of the gametophyte of liver moss is based on 0.2~0.3cm.Finally, the pH value of regulation liquid is Neutral or faintly acid, obtains liver moss stoste.
3rd step, the liver moss stoste of preparation is transferred to the green carving skeleton of sterilizing, and this example is a length of of green carving skeleton It is 15cm, 10cm wide, high 12 centimetres.
First, after by the skeleton first soaked cleaning of green carving, timing sterilization or use sunlight photograph in the sterilized solution of constant volume are put into Capable sterilizing is injected, green carving skeleton can keep dry state after illumination sterilizing, be more beneficial for carrying out the transfer operation of liver moss stoste.Its It is secondary, then green carving skeleton is cleaned with clear water, green carving skeleton is kept moisture state.Finally, liver moss stoste is brushed to green carving skeleton On, it is preferred that so that the liver moss stoste brushed in green carving skeleton surface reaches the state not slid.Green carving skeletal back depression Place, while transplanting has 1 plant of 10 centimetres of Selaginella tamariscinas high.
4th step, the green carving skeleton that will be covered with the liver moss stoste is positioned in breeding device.
In the present embodiment, breeding device by breeding box, mesh-supported frame, reflective membrane, by light transmissive material make breeding cover, Circular arc reflector is constituted.The cover top of the breeding cover is provided with air-vent, and water is filled in the breeding box, and the mesh-supported sets up It is placed in the breeding box and away from the water surface, the reflective membrane is layed on support frame, the green carving skeleton is positioned over the net On shape support frame reflective membrane, the breeding cover is buckled in the breeding box and stretched under the water surface.Breeding box 65cm long, width 38cm, high 15 centimetres, breeding cover 54cm long, 28cm wide, high 25 centimetres, mesh-supported frame is laid away from 3 centimetres of the water surface on support frame There is reflective membrane.Arc plate is made up of plastic not easy rusting material in the circular arc reflector, such as metallic plate or plastic plate, this use 0.2 millimeters thick aluminium zine plate, makees 45 centimetres wide 15 centimetres of length after flanging reinforcement treatment, then is converted into circular arc reflector, the relatively strong letter of illumination Direct aluminium zine plate true qualities are reflective under easily picking up cool canopy or tree shade, and illumination is weaker simply to pick up the bonding on aluminium zine plate under cool canopy or tree shade Or with clip to fix same aluminium zine plate size reflective membrane reflective;The reflective board of circular arc and by located at breeding box side and forming reflecting angle It is 70 degree.The reflective breeding device of split is not limited to this example specification.In order to know the reflective breeding of split Temperature and humidity in device, to be adjusted temperature and humidity.Hygrothermograph is provided with breeding box, using hand-held illumination Degree meter determines the illuminance in device.This example breeding device compares other breeding devices in the case where summer simply picks up cool canopy or tree shade Can be lowered the temperature 3-5 degree more.
A kind of green carving skeleton is additionally provided in the present embodiment, it includes metal net layer 101, surface layer 102 and suture 103, Refering to Fig. 2.Surface layer 102 is laid on metal net layer 101, and metal net layer 101 and surface layer 102 are sutured by the suture 103 It is fixed.Metal net layer 101 has good plasticity, and green carving skeleton is foldable, consolidates so as to solve common green carving frame configuration It is fixed, the defect of not foldable change.When green carving skeleton is made, metal net layer 101 and surface layer 102 are chosen first, by suture Line 103 sutures metal net layer 101 and surface layer 102, metal net layer 101 and surface layer 102 is closely fit together, by folding The green carving skeleton that paper method will have been sutured is folded into variously-shaped.On the other hand, surface layer 102 can be as cultivating such as liver moss Deng the attachment carrier of plant, realize that cultivating moss green carving on green carving skeleton forms various moss green carving moulding.Surface layer 102 needs profit In liver moss stoste attachment and moss growth, such as 3M scouring pads, certainly also can with the geotextiles of other class material such as fibrous materials, Air filtration cellucotton etc..Suture 103 preferably uses nylon wire, and nylon linear elasticity is strong and durable.
5th step, the development direction of liver moss is controlled according to the film-forming state for being covered in the green liver moss stoste for carving skeleton surface Carry out moss cultivation.
The species and component that the film-forming state for being covered in the liver moss stoste on green carving skeleton surface passes through film forming agent are main control. Main control refers to playing the film-forming state of the liver moss stoste on green carving skeleton surface the factor of main, crucial control action It is the species and component of film forming agent, the content of the other compositions in culture medium, the size of liver moss raw material and consumption are also to liver moss The film-forming state of stoste can also play the control action of auxiliary, but play a major role be film forming agent species and component. Using essence of loosening the soil, this brand used is " Heze section of Switzerland strangely-shaped pines soil is smart " to wherein film forming agent, and essence of loosening the soil is not limited to this institute With brand, when same liver moss mixes, spumescence is formed with expansion, therefore with the broken segment length of time control in same machine Longer, the longer then vitality of broken section of liver moss is stronger;Liver moss stoste turns liquid has adhesiveness into film on skeleton, cultivates whole It is difficult to slide, and growth can be beneficial to going the liver moss raw material of water process to play certain stored nutrient jointly;When liver moss on skeleton When stoste dries up accidentally, during using spraying or immersion moisturizing, can be kept the skin wet beneficial to rapid absorption, without like gel-like moisturizing It is difficult;And because it is that water adhesion agent has the accessibility like as water, it is therefore very light to the pressure of liver moss, beneficial to photosynthetic, liver moss emerge compared with Easily.Preferably, can also be by controlling the weight and tongue of water content in culture medium, blue or green moss or white hair moss in liver moss stoste The broken segment length of moss come aid in control film-forming state.
To loosen the soil essence, the culture medium is free of starch component to the film forming agent, and breeding method includes the first cultivating method and the Two cultivating methods, the first cultivating method development direction is using buds to propagate buds;The second cultivating method development direction is protonema;Green carving The film forming of skeleton surface liver moss stoste judges feature:Liver moss stoste is completely covered green carving skeleton surface.
Breeding method includes the first cultivating method and the second cultivating method.
First cultivating method includes using buds to propagate buds induction period and using buds to propagate buds seeding stage:
Principal character in first cultivating method cultivating process:Green carving skeleton surface has young moss seedlings to produce.First cultivating method is trained Foster base crawls volt piece life class liver moss such as blue or green moss 2, and 4-D suitable concentrations are 0.25mg/L, and carbohydrate is suitably 8g/L~12g/L, upright clump Raw class liver moss lignifying is higher if white hair moss NAA (a- methyl α-naphthyl acetates) or a- Nafusakus suitable concentration are 0.01mg/L, and GA3 is suitable Concentration is 1mg/L, and carbohydrate is suitably 28g/L~32g/L, and the Differentiation Types use the first culture medium.
Second cultivating method includes protonema induction period, protonema seeding stage.Principal character in second cultivating method cultivating process: Green carving skeleton surface has a large amount of protonemas to produce, and is produced with a small amount of young moss seedlings;Second cultivating method culture medium white hair moss 2, 4-D suitable concentrations are suitably 28g/L~32g/L for 0.5mg/L carbohydrates, blue or green moss 2,4-D suitable concentrations are 0.1mg/L, and carbohydrate is fitted Preferably 8g/L~12g/L,;
Using buds to propagate buds induction period:20-30 days time, 5-28 DEG C of temperature, preferably 15-25 DEG C, illumination control 3500LX with Under, preferably 1200-2500LX, preferably humidity 60-90%, 70-80%;
The using buds to propagate buds seeding stage:5-28 DEG C of temperature, preferably 15-25 DEG C, illumination control below 3500LX, preferably 1200-2500LX, preferably humidity 60-90%, 70-80%;
Protonema induction period:25-45 days time, 18-25 DEG C of temperature, preferably 20-25 DEG C, illumination control 0-8000LX, Preferably 3500-4500LX, humidity 60-90% is preferably 70-80%;
The protonema seeding stage:5-28 DEG C of temperature, preferably 15-25 DEG C, illumination control below 3500LX, preferably 1200- 2500LX, preferably humidity 60-90%, 70-80%;
First cultivating method crawls volt piece life class bryophyte growth regulator composition can also be as follows:Formula one, 2,4- D0.25mg/L, diethyl aminoethyl hexanoate 1-2mg/L;Formula two, 2,4-D0.25mg/L, the confused sodium 1-1.5mg/L of multiple nitre, formula three, 6- BA0.5-1mg/L, a- Nafusaku 0.25-0.5mg/L, 3- butyric acid potassium 0.25mg/L, diethyl aminoethyl hexanoate 1mg/L etc., or add again Cycocel etc. can be used in production.First cultivating method uprightly grow thickly class liver moss formula one, NAA0.01mg/L, GA3 gibberellin 1mg/ L, diethyl aminoethyl hexanoate 2-3mg/L;Formula two, NAA0.01mg/L, GA3 gibberellin 1mg/L, the confused sodium 2-3mg/L of multiple nitre etc. can be used in life Produce.When plant growth regulator excessive concentration in first cultivating method, when such as using 2,4-D1mg/L to cultivate blue or green moss, in cultivating process Another feature can be also rendered as:Green carving skeleton surface has young moss seedlings to produce, and the liver moss raw material being induced can be rendered as bottle green Callus, can be converted into protonema and be divided into seedling again, and to turn protonema more difficult for callus in this feature, typically without.
In addition, the plant growth regulator in upright culture medium of growing thickly can use as follows:Being formulated the one, concentration of 2,4-D is 0.5mg/L;The concentration of two, 2,4-D is formulated for 0.5mg/L, diethyl aminoethyl hexanoate 1-2mg/L etc. can be used in production.Volt piece of crawling gives birth to class tongue Moss plant growth regulator is constituted can also be as follows:The concentration of formula one, 2,4-D is 0.1mg/L;Being formulated the two, concentration of 2,4-D is 0.1mg/L, diethyl aminoethyl hexanoate 0.5-1mg/L etc. can be used in production.
First and second cultivating methods are operated up to aseptic in greenhouse or tissue culture room without worm, and culture medium can be not added with bactericidal agent and kill Worm agent.
Favorable season of this example in natural environment is the cultivation breeding that spring, summer and autumn carry out liver moss, former Filament induction need to be aided with temperature control measure and reach in summer.Liver moss can use 1/2 concentration cultures composition every with spray-on process before emerging Once make nutritional supplementation within 10-15 days.
By this example water control humiture cool principle, therefore in batch production, breeding apparatus also can use insulation breeding Tank long, support frame, reflector, reflective membrane, pipeline and circulating water pump device, air-supply arrangement, multiple breeding covers are constituted.Breeding cover Size with profit purchase make or operation depending on, depending on reflective membrane and reflective board size radian are with breeding cover, pipeline and recirculated water Pump installation, air-supply arrangement are according to institute's water requirement and amount of moisture sizing number.
Example IV
The present embodiment provides a kind of preparation method the method for moss green carving and comprises the following steps:
The first step, preliminary treatment is carried out by the liver moss raw material of collection:First, collection moss is crawled and lies prostrate the green grass or young crops that piece life is made to represent The gametophyte of moss, is then cleaned with water to remove the impurity such as soil, rubble, withered grass leaf, is then cleaned with washing powder and water. Finally the water in liver moss raw material is extracted makes the gametophyte of blue or green moss in moisture state.
Second step, the culture medium needed for blue or green moss moss growth is mixed with the liver moss raw material after being processed by step (1) Liver moss stoste:
Culture medium:Culture medium includes nutrient mother liquor, plant growth regulator mother liquor, the desinsection needed for moss growth Agent, anti-browning agent, antipollution, carbohydrate, yeast juice and mixed type artificial endosperm.Specifically, the nutrition unit needed for moss growth Plain mother liquor:Prepared with KNOP, potassium nitrate 250mg/L, potassium dihydrogen phosphate 250mg/L, calcium nitrate 1000mg/L, sulfuric acid Magnesium 250mg/L, zinc sulfate 3mg/L.Plant growth regulator mother liquor is prepared with following ratio:6-BA 0.5-6mg/L are used 0.1moL NaOH warm water hydrotropy solutions, a- Nafusaku 0.25-3mg/L warm water hydrotropy solutions, 3- butyric acid potassiums 0.25-1.5mg/L Warm water hydrotropy solution, DA-6 diethyl aminoethyl hexanoate 1-6mg/L warm water hydrotropy solutions.Bactericide first just reaches bright shield 1:1000 (pesticide-seeds ratios), insecticide Chitosan 1:600 (pesticide-seeds ratios), carbohydrate such as white sugar 12g/L, ascorbic acid 2mg/L, Angel Yeast 0.32g/L mother liquors, activated carbon 0.7 gram/L.Mixed type artificial endosperm mixed by by the soaked making of film forming agent, then by starch component and film forming agent and Into.Wherein, the first cultivating method film forming agent essence of loosening the soil, starch component is that the potato fritter of fritter is cut through peeling.Second cultivating method Film forming agent sodium alginate or preferably using 8 one-tenth sodium alginates with 2 one-tenth loosen the soil essence compounds, starch component is tapioca starch.
Prepare liver moss stoste:First, needed for above-mentioned moss growth nutrient mother liquor, plant growth regulator mother liquor, Insecticide, anti-browning agent, anti-pollutant, carbohydrate and yeast juice are mixed in mixer.Secondly, and in the mixer add Blue or green moss, water make the temperature of liquid be 25 DEG C or so, again, 1-2 points are stirred together to mixed type artificial endosperm is added in mixer Clock, it is preferable that the broken segment length of the gametophyte of blue or green moss is that the first cultivating method film forming agent starch is such as based on 0.25-0.35cm Without filtering potato grain, juice, wherein potato grain is preferred with reaching diameter about 0.1cm;Second cultivating method film forming agent, it is preferable that adopt With 8 one-tenth sodium alginates with 2 one-tenth compounds of essence of loosening the soil, starch component is tapioca starch.
Finally, the pH value of regulation liquid is 6.8-7, obtains liver moss stoste.
3rd step, the liver moss stoste of preparation is transferred to the green carving skeleton of sterilizing, a length of 15cm of the green carving skeleton of this example, It is 7cm wide, high 12 centimetres.
First, after by the skeleton first soaked cleaning of green carving, timing sterilization or use sunlight photograph in the sterilized solution of constant volume are put into Inject capable sterilizing.Next, then green carving skeleton is cleaned with clear water, green carving skeleton is kept moisture state.Finally, by liver moss stoste brush It is coated onto on green carving skeleton, it is preferred that so that the liver moss stoste brushed in green carving skeleton surface reaches the state not slid.
4th step, the green carving skeleton that will be covered with the liver moss stoste is positioned in breeding device.
In the present embodiment, the reflective breeding device of buckle-type is by buckle-type breeding box, mesh-supported frame and by light transmissive material The breeding closure assembly of making, seedlings nursing plate, circular arc reflector are constituted.The buckle-type breeding box is by 30 centimetres of white diameter, a height of 2 Centimetre plastic feet forms circular breeding basin and reflective using white true qualities, and further the weaker place of illumination can also be at breeding basin bottom With the week side of boss lay reflex film, 27 centimetres of transparent plastic circle flat-top diameter, 15 centimetres of formation breeding cover high;The cover top of the breeding cover Flat-top sidepiece is provided with air-vent,;Dry season fills water in the breeding box basin, and overcast and rainy season is not filled with water;The mesh-supported Erection is placed in the breeding box basin and away from water surface 3cm, and the green carving skeleton is positioned on the mesh-supported frame, described to educate Plant cover button and seam is detained in Kou Fengpen located at the breeding box.The seedlings nursing plate is that plastic material is square, and bottom is in the sieve that gathers Shape hole, 39 centimetres of diameter is high 5.5 centimetres, 42.5 centimetres of top diameter.
Arc plate is made up of plastic not easy rusting material in the circular arc reflector, such as metallic plate or plastic plate, this use 0.2 millimeters thick aluminium zine plate, makees 45 centimetres wide 13 centimetres of length after flanging reinforcement treatment, then is converted into circular arc reflector, and illumination is set more by force Shade is lower directly reflective with aluminium zine plate true qualities, and bonding or same aluminium zine plate size is fixed with clip on aluminium zine plate under the weaker tree shade of illumination Reflective membrane is reflective;2 diameters, 1 centimetre of aperture, apart 2 are respectively opened far from 12 centimetres of side bottom, 1 centimeters high in the circular arc reflector left and right sides Centimetre;Circular arc reflector blocks and by located at seedlings nursing plate side, using rope perforation is by reflector and educates away from 2 centimetres on one side of seedlings nursing plate Seed plate is fixed, and regulation reflecting angle is 75 degree, and regulation angle can directly block by being formed, and gravelstone or wooden unit can be also added after reflector Support regulation angle.
The reflective breeding device of buckle-type is not limited to this example specification, shape, such as fastened in this example Breeding cover can also be spheroidal in the reflective breeding device of formula, and breeding box and cover can also be rectangular arch etc., according to green carving skeleton rule Lattice dimension adjustable is from the reflective breeding device of buckle-type, seedlings nursing plate, the circular arc reflector for being accordingly adapted to green carving moss growth; Reflectorized material entirety or split can be added to be molded with materials such as plastics, shape is constituted to meet the reflective breeding device function of the buckle-type Formula occurs, and size, shape is not limited, but with certain aesthetic property, cultivate green carving for family DIY or sightseeing type factory cultivates green Carving.The reflective breeding device of buckle-type is served and freely adjust light filling angle and multi-angle light filling, and without periodically exchanging green carving again Light angle effect, rain-proof, anti-ponding, profit growth, sharp transportation, are adapted to the outdoor green carving of large scale cultivating.And it is indoor without anti- Rainwater, in the reflective breeding device of buckle-type:Breeding cover to can select and open air-vent at the top of dome, seedlings nursing plate can bottom it is non-porous.
The reflective breeding device of buckle-type applies also for following breeding method in addition to this example:Be free of suitable for the culture medium Starch component, the development direction is that using buds to propagate buds cultivates green carving;The development direction of the liver moss is that protonema cultivates green carving;Institute The development direction of liver moss is stated for callus cultivates green carving.
5th step, the development direction of liver moss is controlled according to the film-forming state for being covered in the green liver moss stoste for carving skeleton surface Carry out moss cultivation.
It is covered in species and component and starch that the film-forming state of the liver moss stoste on green carving skeleton surface passes through film forming agent Composition is main control, it is preferable that by controlling the volume and weight and green grass or young crops of water content in culture medium, blue or green moss in liver moss stoste The broken segment length of moss come aid in control film-forming state.Made with the species of film forming agent, component and starch constituents in the present embodiment For the film-forming state of liver moss stoste is major control factors, its specific consumption, species are studied.The development direction It is using buds to propagate buds.First cultivating method film forming agent essence of loosening the soil, starch component is murphy juice, grain.Second cultivating method film forming agent uses 8 Into sodium alginate with 2 one-tenth compounds of essence of loosening the soil, starch component is tapioca starch.Two kinds of cultivating method mixed type artificial endosperms of this example Application target is all further to make skeleton surface stoste state be rendered as, with certain honeycomb cavernous structure, easing off the pressure, Increase gas permeability, make to emerge relatively easily, and reduced because of the addition of starch material cultivation in spray nutrition number of times, even up to it is green Product are carved all without supplementing the nutrients.
Film forming judges feature:Liver moss stoste is completely covered green carving skeleton surface;
, there is one of following two kinds of features in the breeding method in principal character in cultivating process:
First cultivating method feature:Liver moss stoste was transferred to green carving skeleton surface after 1-3 days, was assigned exposed to illumination comprehensively dry It is dry to be sterilized, green carving skeleton surface has young moss seedlings to produce;
Second cultivating method feature:Liver moss stoste sterilizes after being transferred to green carving skeleton surface without exposure under illumination, green carving Skeleton surface has young moss seedlings to produce;
Using buds to propagate buds cultivating process includes using buds to propagate buds induction period and using buds to propagate buds seeding stage;
Using buds to propagate buds induction period includes one of following two kinds of breeding methods:
First cultivating method:Liver moss stoste was transferred to green carving skeleton surface after 1-3 days, after preferably 2 days, was then exposed to light Dried to be sterilized comprehensively according to assigning, then soak again or spray water and put shady place, it is continuous to cultivate 20-30 days;
Second cultivating method:It is continuous to cultivate 20-30 days after liver moss stoste is transferred to green carving skeleton surface;
The breeding condition of the first cultivating method and the second cultivating method is 5-28 DEG C of temperature, preferably 15-25 DEG C, illumination control Below 3500LX, preferably 1200-2500LX, preferably humidity 60-90%, 70-80%;
The using buds to propagate buds seeding stage:5-28 DEG C of temperature, preferably 15-25 DEG C, illumination control below 3500LX, preferably 1200-2500LX, below 15000-25000LX sunlight helps growth, humidity 60- in 1-2 times weekly, morning or/and 1 hour evening 90%, preferably 70-80%.Early, evening carries out intense light irradiation in short-term can rise in cultivating process because helping sterilization functions containing organic matter. This example suitable cultivation season in natural environment is spring, autumn.
Embodiment five
The present embodiment provides a kind of preparation method the method for moss green carving and comprises the following steps:
The first step, preliminary treatment is carried out by the liver moss raw material of collection:First, collection moss uprightly grow thickly make represent it is white The gametophyte of hair moss, is then cleaned with water to remove the impurity such as soil, rubble, withered grass leaf, is then carried out clearly with washing powder and water Wash.Finally the water in liver moss raw material is extracted makes the gametophyte of white hair moss in moisture state.
Second step, tongue is mixed by the culture medium needed for the growth of white hair moss and the liver moss raw material after being processed by step (1) Moss stoste:
Culture medium:Including the nutrient mother liquor needed for moss growth, plant growth regulator mother liquor, insecticide, anti-brown Become agent, anti-pollutant, carbohydrate, yeast juice and single type artificial endosperm.Specifically, needed for moss growth nutrient is female Liquid:Prepared with KNOP, potassium nitrate 250mg/L, potassium dihydrogen phosphate 250mg/L, calcium nitrate 1000mg/L, magnesium sulfate 250mg/L, zinc sulfate 3mg/L.Plant growth regulator mother liquor is prepared with following ratio:6-BA 1-6mg/L 0.1moL NaOH warm water hydrotropy solutions, a- Nafusaku 0.5-2mg/L warm water hydrotropy solutions, 3- butyric acid potassium 0.25-1mg/L warm water hydrotropies Solution, compound sodium nitrophenolate 1-6mg/L warm water hydrotropy solutions.Bactericide first just reaches bright shield 1:1200 (pesticide-seeds ratios), insecticide chitosan 1:800 (pesticide-seeds ratio), carbohydrate such as white sugar 30g/L, ascorbic acid 2mg/L, Angel Yeast 0.32g/L, activated carbon 0.7-1.2g/L.Mixing Type artificial endosperm by by film forming agent it is soaked or stirring be made, then starch component is mixed with film forming agent, film forming Agent water-loss reducer or water-loss reducer and the smart compound that loosens the soil, water-loss reducer is 9 with the mass ratio of essence of loosening the soil:1, starch component is Without filtering murphy juice.
Prepare liver moss stoste:First, needed for above-mentioned moss growth nutrient mother liquor, plant growth regulator mother liquor, Insecticide, anti-browning agent, anti-pollutant, carbohydrate and yeast juice are mixed in mixer.Secondly, and in the mixer add White hair moss, water make the temperature of liquid be 25 DEG C, stir 1 minute.Again, mixed type artificial endosperm and stir to being added in mixer 1-2 minutes, it is preferable that the broken segment length of the gametophyte of white hair moss is 0.1cm.Finally, the pH value of regulation liquid is 6-7, preferably It is 6.8-7, obtains liver moss stoste.
3rd step, the liver moss stoste of preparation is transferred to the green carving skeleton of sterilizing, and this example is a length of of green carving skeleton It is 15cm, 10cm wide, high 12 centimetres.
First, after by the skeleton first soaked cleaning of green carving, timing sterilization or use sunlight photograph in the sterilized solution of constant volume are put into Capable sterilizing is injected, green carving skeleton can keep dry state after illumination sterilizing, be more beneficial for carrying out the transfer operation of liver moss stoste.Its It is secondary, then green carving skeleton is cleaned with clear water, green carving skeleton is kept moisture state.Finally, liver moss stoste is brushed to green carving skeleton On, it is preferred that so that the liver moss stoste brushed in green carving skeleton surface reaches the state not slid.
4th step, the green carving skeleton that will be covered with the liver moss stoste is positioned in breeding device.
In the present embodiment, breeding device:The breeding cover structure made by breeding box, mesh-supported frame and by light transmissive material Into the cover top of the breeding cover is provided with air-vent, and water is filled in the breeding box, and the mesh-supported sets up and is placed in the breeding In case and away from the water surface, the green carving skeleton is positioned on the mesh-supported frame, and the breeding cover is buckled located at the breeding box Side button seam.Breeding box 54cm long, 28cm wide, high 6 centimetres, mesh-supported frame is away from 3 centimetres of the water surface.Mesh-supported frame 47cm long, width 23cm, 3cm high.
5th step, the development direction of liver moss is controlled according to the film-forming state for being covered in the green liver moss stoste for carving skeleton surface Carry out moss cultivation.
Film-forming state is main control by the species of film forming agent, component and starch component, it is preferable that by controlling to cultivate Water content, white hair moss volume and weight in liver moss stoste and the broken segment length of white hair moss aid in controlling film-like in base State.The film forming agent is the compound of water-loss reducer or water-loss reducer and essence of loosening the soil, and the culture medium contains starch component, the hair Direction is educated for callus;
Film forming judges feature:Liver moss stoste is completely covered green carving skeleton surface and with certain reflective;In this example, tool Having reflective then can prevent germ contamination in cultivating process, and can prevent certain condensed water, and can make liver moss stoste in cultivation Do not slide, preserve nutrition, at the same it is therefore big to liver moss pressure, force it to be sent out towards callus direction in the case of hot and humid Educate;This example film forming agent is played beneficial to gelatification is stirred into using compound, and water-loss reducer will not flash machine week in making stirring Wall, and cleaning robot is easier.Broken section of control is thinner, beneficial to making callus easily be divided into protonema, and may occur in which a small amount of tongue Moss seedling.Certainly in this example, if broken section of control of liver moss can also ensure that Cultivated objective is unified out to be entirely at 0.2-0.35 centimetres Callus, but break up more difficult, therefore do not use typically.
Principal character in cultivating process:Green carving skeleton surface has a large amount of callus and a small amount of bud to produce after induction period;More Injured tissue cultivating process includes that callus induction phase, callus turn protonema and protonema seeding stage;
The callus induction phase:20-30 days time, 20-30 DEG C of temperature, illumination control below 2500LX, humidity 70-90% Preferably 80-90%;
Callus turns the protonema phase:25-45 days time, 15-25 DEG C of temperature, preferably 18-22 DEG C, illumination control 3500-15000LX, preferably 3500-8000LX, humidity 60-90% are preferably 70-80%.
The protonema seeding stage:5-28 DEG C of temperature, preferably 15-25 DEG C, illumination control below 3500LX, preferably 1200- 2500LX, the illumination that green carving skeleton was changed per 15-20 days receives direction, and humidity 60-90% is preferably 70-80%.
This example favorable season in the natural environment is to play breeding in autumn September.This example can be with 20-30 days one before emerging 1/2 concentration cultures nutritional ingredient of secondary supplement.

Claims (9)

1. a kind of preparation method of moss green carving, it is characterised in that comprise the following steps:
(1) the liver moss raw material of collection is carried out into preliminary treatment;
(2) culture medium needed for moss growth is mixed into liver moss stoste with the liver moss raw material after being processed by step (1);
(3) make green carving skeleton and the liver moss stoste is transferred to the green carving skeleton by sterilizing;
(4) the green carving skeleton that will be covered with the liver moss stoste is positioned in breeding device, and the breeding device includes following dress One of put:
The reflective breeding device of buckle-type:By breeding box, mesh-supported frame, reflex reflector and the breeding by light transmissive material making Cover is constituted, and the cover top of the breeding cover or side top are provided with air-vent, and water is filled in the breeding box, and the mesh-supported frame is set In in the breeding box and away from the water surface, the green carving skeleton is positioned on the mesh-supported frame, and the breeding cover is buckled and is located at The side seam of the breeding box;The reflex reflector is made up of reflective membrane and reflector, and the reflective membrane is layed in breeding chamber interior wall And the week side of boss, the reflector is outside breeding box;
The reflective breeding device of split:By breeding box, support frame, reflex reflector and the breeding cover structure by light transmissive material making Into the cover top of the breeding cover or side top are provided with air-vent, and water is filled in the breeding box, and support frame as described above is arranged at described educating Plant in case and away from the water surface, the green carving skeleton is positioned on support frame as described above, and the breeding cover is buckled in the breeding box And stretch under the water surface;The reflex reflector is made up of reflective membrane and reflector, and the reflective membrane is layed on support frame or spreads Located at breeding box inner circumferential side, the reflector is in breeding box or outside breeding box;
(5) the development direction of liver moss is controlled according to the film-forming state for being covered in the green liver moss stoste for carving skeleton surface, tongue is carried out Moss is cultivated.
2. the preparation method of moss green carving according to claim 1, it is characterised in that the described preliminary place in step (1) Reason includes following operation:
(1.1) removal of impurity is gone to the liver moss raw material cleaning for gathering first;
(1.2) sterilization treatment is carried out to the liver moss raw material gone after the removal of impurity, water process then is carried out to liver moss raw material, make liver moss Raw material is in moisture state.
3. the preparation method of moss green carving according to claim 1, it is characterised in that the culture medium in step (2) Including following composition:
(2.1) nutrient mother liquor needed for moss growth, plant growth regulator mother liquor, insecticide, anti-browning agent, antipollution Agent, carbohydrate and yeast juice;
(2.2) single type artificial endosperm or mixed type artificial endosperm:
Single type artificial endosperm:By film forming agent it is soaked or stirring be made;Mixed type artificial endosperm:It is film forming agent is soaked or stir Mix, then starch component is mixed with film forming agent.
4. the preparation method of moss green carving according to claim 3, it is characterised in that the liver moss in step (2) is former The preparation of liquid includes multi-step mixing facture or synchronous stirring facture:
Multi-step mixing facture:Each component in step (2.1) is mixed in container, the temperature for making liquid to being added water in container is 20 DEG C~45 DEG C, stirred 1-2 minutes in being then put into mixer together with the liver moss raw material by preliminary treatment;Again by making Single type artificial endosperm or mixed type artificial endosperm are put into mixer, are stirred for 1-2 minutes;Adjusted to pH value is added in mixer Agent obtains liver moss stoste to adjust pH value as neutral or faintly acid;
Synchronous stirring facture:Each component in step (2.1) is mixed in container, the temperature for making liquid to being added water in container is 20 DEG C~45 DEG C, then it is put into and stirs with the liver moss raw material by preliminary treatment, single type artificial endosperm or mixed type artificial endosperm Mix in machine, stir 0.5-2 minutes;Again to adding pH value regulator to adjust pH value as neutral or faintly acid in mixer, obtain To liver moss stoste.
5. the preparation method of moss green carving according to claim 1, it is characterised in that the green carving bone of making in step (3) The green carving skeleton that the liver moss stoste is transferred to by sterilizing simultaneously is included following operation by frame:
(3.1) make green carving skeleton and by the skeleton first soaked cleaning of green carving, be then placed in timing sterilization in the sterilized solution of constant volume Or sterilized by illumination, the green carving skeleton includes metal net layer, surface layer and suture;The surface layer is laid in the gold On category stratum reticulare, the metal net layer and the surface layer are sutured by the suture and fixed;
(3.2) green carving skeleton is made to keep moisture state afterwards with the green carving skeleton after clear water drip washing sterilization treatment again;
(3.3) liver moss stoste is transferred in the green carving skeleton of moisture state so that the liver moss for being covered in green carving skeleton surface is former Liquid reaches and does not slide, and the method for the transfer includes any one or more in spraying, brushing, dip-coating.
6. according to the preparation method of the described moss green carving of one of claim 1 to 5, it is characterised in that described in step (5) Film-forming state is main control by the species of film forming agent, component and starch component, and the film forming agent is water-loss reducer or water-loss reducer With the compound of essence of loosening the soil, the culture medium contains starch component, and the development direction of the liver moss is protonema;
Film forming judges feature:Liver moss stoste is completely covered green carving skeleton surface, and with reflective;
Principal character in cultivating process:The viridescent protonema in green carving skeleton surface is produced;
The cultivating process of protonema includes protonema induction period and protonema seeding stage;
Protonema induction period:20-35 days time, except 18-25 DEG C of fine day outer control temperature, intensity of illumination is 0-8000LX, humidity 75-97%;Within 30 DEG C, can be spaced carries out a fine day illumination for one week to temperature at the green carving skeleton of fine day control, and illumination is strong Degree control is in below 12000LX, and continuous sunny need to do shading treatment, cloudy to shelter from heat or light;
The protonema growth seeding stage includes one of following two kinds of breeding methods:
First cultivating method:Green carving surface is rendered as thallus, protonema, cormus, four kinds of states of callus, the outdoor nothing of transfer Heating is cultivated, 5-25 DEG C of temperature, illumination control 0-25000LX, humidity 60-95%;
Second cultivating method:Protonema is divided into thallus state, and transfer is outdoor to cultivate without heating, illumination control 0-4500LX, temperature 5-25 DEG C of degree, humidity 60-90%.
7. according to the preparation method of the described moss green carving of one of claim 1 to 5, it is characterised in that the film forming in step (5) The species and component that state passes through film forming agent are main control, and the film forming agent is essence of loosening the soil, and the culture medium is free of starch component, Breeding method includes the first cultivating method and the second cultivating method, and the first cultivating method development direction is using buds to propagate buds;Described second Cultivating method development direction is protonema;
Film forming judges feature:Liver moss stoste is completely covered green carving skeleton surface;
First cultivating method includes using buds to propagate buds induction period and using buds to propagate buds seeding stage:
Principal character in the cultivating process of the first cultivating method:Green carving skeleton surface has young moss seedlings to produce;
Second cultivating method includes protonema induction period, protonema seeding stage;
Principal character in the cultivating process of the second cultivating method:Green carving skeleton surface has a large amount of protonemas to produce, and with a small amount of tongue Moss seedling produces;
Using buds to propagate buds induction period:20-30 days time, 5-28 DEG C of temperature, illumination control below 3500LX, humidity 60-90%;
The using buds to propagate buds seeding stage:5-28 DEG C of temperature, illumination control below 3500LX, humidity 60-90%;
Protonema induction period:25-45 days time, 18-25 DEG C of temperature, illumination control 0-8000LX, humidity 60-90%;
The protonema seeding stage:5-28 DEG C of temperature, illumination control below 3500LX, humidity 60-90%.
8. according to the preparation method of the described moss green carving of one of claim 1 to 5, it is characterised in that the film forming in step (5) State is main control by the species of film forming agent, component and starch component, and the development direction is using buds to propagate buds;First cultivates Method film forming agent essence of loosening the soil, starch component is potato composition;Second cultivating method film forming agent sodium alginate or sodium alginate and pine The compound of native essence, starch component is tapioca starch;
Film forming judges feature:Liver moss stoste is completely covered green carving skeleton surface;
, there is one of following two kinds of features in the breeding method in principal character in cultivating process:
First cultivating method feature:Liver moss stoste is transferred to green carving skeleton surface after 1-3 days, exposed to illumination assign drying comprehensively with Sterilized, green carving skeleton surface there are young moss seedlings to produce;
Second cultivating method feature:Liver moss stoste sterilizes after being transferred to green carving skeleton surface without exposure under illumination, green carving skeleton Surface has young moss seedlings to produce;
Using buds to propagate buds cultivating process includes using buds to propagate buds induction period and using buds to propagate buds seeding stage;
Using buds to propagate buds induction period includes one of following two kinds of breeding methods:
First cultivating method:Liver moss stoste is transferred to green carving skeleton surface 1-3 days, is then exposed to illumination and assigns comprehensive drying to enter Row sterilizing, then soaks or sprays water and put shady place again, continuous to cultivate 20-30 days;
Second cultivating method:It is continuous to cultivate 20-30 days after liver moss stoste is transferred to green carving skeleton surface;
The breeding condition of the first cultivating method and the second cultivating method includes 5-28 DEG C of temperature, illumination control below 3500LX, humidity 60- 90%;
The using buds to propagate buds seeding stage:5-28 DEG C of temperature, illumination control below 3500LX, humidity 60-90%, weekly 1-2 times with In 15000-25000LX light irradiations 1 hour, to help growth.
9. according to the preparation method of the described moss green carving of one of claim 1 to 5, it is characterised in that in the step (5) Film-forming state is main control by the species of film forming agent, component and starch component, and the film forming agent is water-loss reducer or water-loss reducer With the compound of essence of loosening the soil, the culture medium contains starch component, and the development direction is callus;
Film forming judges feature:Liver moss stoste is completely covered green carving skeleton surface and with reflective;Principal character in cultivating process: Green carving skeleton surface has callus and bud to produce after induction period;
Callus cultivating process includes that callus induction phase, callus turn protonema and protonema seeding stage;
The callus induction phase:20-30 days time, 20-30 DEG C of temperature, illumination control below 2500LX, humidity 70-90%;More Injured tissue turns the protonema phase:25-45 days time, 15-25 DEG C of temperature, illumination control 3500-15000LX, humidity 60-90%;It is former The filament seeding stage:5-28 DEG C of temperature, illumination control below 3500LX, humidity 60-90%.
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CN109618854A (en) * 2019-01-25 2019-04-16 福建师范大学福清分校 The method of moss is cultivated on a kind of charcoal

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