CN104774087A - Biological preparation, its preparation method, and its application in soil improvement and fertilizers - Google Patents
Biological preparation, its preparation method, and its application in soil improvement and fertilizers Download PDFInfo
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Abstract
The invention discloses a biological preparation, its preparation method, and its application in soil improvement and fertilizers. The biological preparation comprises, by mass, 4-5 parts of alfalfa powder, 2-3 parts of aloe powder, and 2-3 parts of soybean powder. The biological preparation prepared by adopting the above scheme is a soil improvement preparation having the advantages of no need of strains, use convenience, long shelf life, no secondary pollution and no hidden safety trouble, has very high application values, and is suitable for being applied in soil improvement and fertilizers. The invention also provides the preparation method of the biological preparation.
Description
Technical field
The present invention relates to the soil improvement without the need to additionally adding bacterial classification, in particular, a kind of biotechnological formulation and preparation method thereof and the application in soil improvement, fertilizer.
Background technology
Soil is the basic substance that the mankind depend on for existence.China's land resources is very limited, and causes the soil with obstruction factor also to there is significant proportion due to nature or human factor.In April, 2014, Chinese Ministry of Environmental Protection combines with Ministry of Land and Resources and has issued 2005 ~ 2013 years national Soil Pollution Investigation publications, and result shows: in factual survey area 6,300,000 square kilometres, the total exceeding standard rate of national soil is 16.1%; Some areas soil pollution is heavier, and wherein the heavy metal pollution problem such as cadmium, mercury, arsenic, copper is given prominence to.
Severe pollution problem has expedited the emergence of soil pollution and has repaired industry.Traditional soil improvement agent raw material type is more numerous and diverse, include natural crystal as montmorillonite, rhombspar, potassium felspar sand, Rock Phosphate (72Min BPL) etc., natural active matter is as biochemical fulvic acid etc., agriculture and industry waste is as gourmet powder fermenting tail washings etc., and artificial-synthetic copolymer is as lauryl alcohol oxyethyl group ammonium sulfate, polymaleic acid etc.These soil conditioners improving Soil structure, regulate soil pH value, serve certain effect in improvement saline-alkali soil, repairing polluted soil etc., but due to conventional soil modifying agent self, there is much potential environmental risk in it.From consumption, conventional conditioner consumption is general comparatively large, and needs repeatedly or use in many seasons, probably to cause secondary pollution.Especially solid waste and high molecular polymeric conditioners, if harmless treatment is incomplete, likely causes these wastes to transfer to agriculture production, becomes the starting point of food chain.In addition, the positively charged ion that the amendment in some natural mineral source discharges after decomposing also may produce toxic action to soil.
Along with the development of biotechnology, the microorganism remediation technology of soil is more and more active in recent years.Because microorganism amendment can bring certain fertilizer efficiency usually, be therefore usually also microbial fertilizer.The microbial fertilizer of narrow sense refers to the vital movement by wherein contained microorganism, increases the supply of plant nutrient, improves the nourishment of plant, and then increase yield; The microbial fertilizer of broad sense is then not limited only to the level of supply improving plant nutrient, its goods are except passing through the vital movement promotion plant growth of wherein microorganism, also comprise the secondary metabolites that they produce, if hormonal substance is to the hormesis of plant, promote absorbing of Plant To Nutrient element, or can the pathogenic effects of some pathogenic micro-organism of antagonism, alleviate disease and pest and make crop yield.
The development of China Microbiological fertilizer industry through decades has achieved certain achievement, reaches a certain scale, for important contribution has been made in Chinese agriculture production, agriculture Sustainable development and environment protection etc.But current China Microbiological fertilizer industry still also exists that integral level is not high, technological innovation is not enough, quality product and the problem such as effect is unstable, market management is chaotic.Specifically, subject matter is as follows:
1, bacterial classification easily morphs.Current microbial fertilizer is in the majority with bacteria fertilizer, and bacterium has stronger automutagenicity, therefore in bacterial strain breeding succeeding generations, often causes weakening of strain characteristic even to disappear.So a lot of bacterial fertilizer has good effect when research and development and test, but in promoting the use of, often tell on the phenomenon reduced.
2, easily living contaminants is had in production process.In factorial praluction, generally lack bacterial strain, microorganism resource is deficient, is often use identical bacterial strain in a lot of product.And number of viable is few, miscellaneous bacteria is more, and validity period is short.In addition, some harmful bacterial strain without qualification flows into production link, brings potential safety hazard.
3, in preservation process, effective bacterium rate of decay is fast.There are some researches show, from the bacterial manure that the excellent growth-promoting bacterial strain of herbage rhizosphere screening makes, effective bacterium number was decayed 10 times after 3 months, decayed 107 times after six months, decayed 108 times afterwards in 1 year.Therefore, bacterial manure validity period is shorter, and validity can be successively decreased along with the time.
4, the incompatibility phenomenon that produces after entering soil of external source bacterium.Bacterial manure is generally make after utilizing culture propagation, applies in different soil, is likely not suitable with the edatope on this ground, is difficult to preserve its activity.In addition, external bacterial classification entered environment, likely can cause certain specified microorganisms rapid appreciation, disturb ecological stability.
5, for the soil of different situations, need to choose different bacterial classifications and bacterial manure, Application comparison bothers, and the time length is short, and effect is unstable.
Therefore, how to realize without the need to bacterial classification, easy to use, long quality-guarantee period, do not produce secondary pollution and without the soil improvement preparation of potential safety hazard, be the technical problem being badly in need of solving.
Summary of the invention
The invention provides a kind of new biotechnological formulation and preparation method thereof and the application of this biotechnological formulation in soil improvement, fertilizer.
A technical scheme of the present invention is as follows: a kind of biotechnological formulation, and it comprises the component of following quality: alfalfa meal 4-5 part, aloe powder 2-3 part, soyflour 2-3 part.
Another technical scheme also comprises marine alga.
Another technical scheme is, also comprises marine alga 0.4-1.6 part.
Another technical scheme is, alfalfa meal: aloe powder: soyflour is 4:3:3 to 2:1:1.
Another technical scheme is, described biotechnological formulation also comprises water at low temperature 15 to 190 parts.
Another technical scheme is, water at low temperature is temperature lower than the Natural Water of 30 degrees Celsius.
Another technical scheme is, the mixture of alfalfa meal, aloe powder and soyflour adopts the extraction of water at low temperature non-fermented.
Another technical scheme is, described biotechnological formulation also comprises seaweed powder 0.5-1.5 part.
Another technical scheme is, described marine alga is brown alga.
Another technical scheme is, described marine alga is bladder wrack.
Another technical scheme of the present invention is as follows: a kind of preparation method of biotechnological formulation, and it comprises the following steps: alfalfa meal, aloe powder and soyflour are carried out low-temperature extraction by the mass ratio of 4-5:2-3:2-3.
Another technical scheme is, a kind of preparation method of biotechnological formulation, and it comprises the following steps: for arbitrary above-mentioned biotechnological formulation, and alfalfa meal, aloe powder and soyflour are carried out low-temperature extraction by the mass ratio of 4-5:2-3:2-3.
Another technical scheme is, alfalfa meal, aloe powder and soyflour is carried out low-temperature extraction by the mass ratio of 4-5:2-3:2-3, adds a small amount of marine alga more afterwards.
Another technical scheme is, adds a small amount of marine alga according to the volume of extraction liquid.
Another technical scheme is, adds freeze-drying seaweed powder according to the volume of extraction liquid.
Another technical scheme is, according to the volume of extraction liquid, by the mass volume ratio example of 5-20g/1L, adds freeze-drying seaweed powder.
Another technical scheme is, described marine alga is bladder wrack.
Another technical scheme is, adopts water at low temperature extraction alfalfa meal, aloe powder and soyflour.
Another technical scheme of the present invention is as follows: the application of arbitrary above-mentioned biotechnological formulation in soil improvement.
Another technical scheme is, the concentration of described biotechnological formulation is 5%-40%.
Another technical scheme is, the consumption of described biotechnological formulation is 20-100mL/ mu.
Another technical scheme of the present invention is as follows: the application of arbitrary above-mentioned biotechnological formulation in fertilizer.
Another technical scheme is, described application is applied to the farm crop of melon and fruit class, Cereal or greengrocery.
Adopt such scheme, the invention provides without the need to bacterial classification, easy to use, long quality-guarantee period, do not produce secondary pollution and without the soil improvement preparation of potential safety hazard, there is very high using value, be applicable to soil improvement and fertilizer application; Present invention also offers the preparation method of this biotechnological formulation.
Accompanying drawing explanation
Fig. 1 is the schematic flow sheet of an embodiment of preparation method of the present invention.
Embodiment
For the ease of understanding the present invention, below in conjunction with the drawings and specific embodiments, the present invention will be described in more detail.It should be noted that, when element is stated " being fixed on " another element, it can directly on another element or can there is one or more element placed in the middle therebetween.When an element is stated " connection " another element, it can be directly connected to another element or can there is one or more element placed in the middle therebetween.The term " vertical " that this specification sheets uses, " level ", "left", "right" and similar statement are just for illustrative purposes.
Unless otherwise defined, all technology of using of this specification sheets and scientific terminology are identical with belonging to the implication that those skilled in the art of the present invention understand usually.The object of term used in the description of the invention in this specification sheets just in order to describe specific embodiment is not for limiting the present invention.The term "and/or" that this specification sheets uses comprises arbitrary and all combinations of one or more relevant Listed Items.
The basic concept of the present invention and each embodiment thereof is, do not add any external source bacterial classification, utilize pure plant extracts to transmit the signal of a suitable growth to soil microorganisms, thus promote the active of soil microorganisms, particularly plant growth-promoting rhizobacteria (PGPR) and breeding.After using this product, alfalfa meal, aloe powder and soyflour provide good growth key element to microorganism, therefore, can set up rapidly huge useful edaphon in soil, thus provide good ecotope for crop root growth.Meanwhile, the healthy growth of root system of plant, also can provide more nutritive ingredient for soil microorganisms conversely, thus forms the benign cycle of a healthy soil-healthy crop.
The present invention and each embodiment thereof relate generally to the biotechnological formulation of comprehensive improvement soil, its making method is by core material, comprise alfalfa meal, aloe powder and soyflour, low-temperature extraction is carried out in certain mass ratio, preferably, in the end add a small amount of organic marine alga, need not microorganism be added, also need not ferment.The present invention and each embodiment thereof have comprehensively improves soil physico-chemical property, improve utilization rate of fertilizer, the effects such as Promoting plant growth, can be used for hardening, the improvement of the degenerated soil such as saline and alkaline etc., desertification, and the common fertilizer that also can be used as the crops such as melon and fruit, cereal, vegetables is used.One embodiment of the present of invention are as follows: a kind of biotechnological formulation, and it comprises the component of following quality: alfalfa meal 4-5 part, aloe powder 2-3 part, soyflour 2-3 part.Such as, every part is 0.1g, 0.5g, 1g, 2g, 3g, 5g, 10g, 20g, 50g, 100g, 500g, 1000g, 5000 grams or 10 kilograms etc., by that analogy.Preferably, each pulvis, comprise alfalfa meal, aloe powder, soyflour and/or wheat-flour etc., its particle diameter is less than 0.45 millimeter.And for example, the particle diameter of each pulvis is less than 0.3 millimeter.
Another embodiment is, the composition of biotechnological formulation of the present invention can be prepared into the water-soluble preparation of applicable agricultural use, and in preparation, the total content of effective constituent is 5%-40%.
Another embodiment also comprises water, and such as, the concentration of biotechnological formulation is 5% to 20%.Such as, 5g biotechnological formulation is placed in 95g water, and its concentration is 5%.Preferably, water at low temperature extraction alfalfa meal, aloe powder and soyflour is adopted.In the liquid obtained after extraction, the concentration of biotechnological formulation is 5% to 20%.And for example, a kind of biotechnological formulation, it comprises the component of following quality: by alfalfa meal 4-5 part, aloe powder 2-3 part and soyflour 2-3 part, be placed in 15 to 190 parts of water at low temperature, the extraction liquid obtained after extraction.Such as, by alfalfa meal 4 parts, aloe powder 2 parts and soyflour 2 parts, be placed in 100 parts of water at low temperature, the extraction liquid obtained after extraction.
By having carried out a large amount of, test of long duration to different plant and different ratio, find that the actives mass-energy by alfalfa meal, aloe powder and soyflour carry out low-temperature extraction gained by the mass ratio of 4-5:2-3:2-3 effectively promotes that soil inside source microbial growth is bred.And for example, also add marine alga can supplement needed for some healthy trace elements with household confession plant-growth.Such as, marine alga is bladder wrack, and wherein containing N.F,USP MANNITOL, fucoidin, Lalgine, amino acid isoreactivity composition, wherein fucoidin has moisture-keeping function, and heavy metal and other toxin have cloudy suction effect; Lalgine is a kind of natural chelators, the formation of energy stimulating growth Auto-regulator and natural antibiotics; Amino acid can promote the synthesis of protein and the stalwartness of plant tissue organ.
Another embodiment also comprises marine alga.Such as, described marine alga is brown alga.Such as, described marine alga is sea-tangle, sargassun, bladder wrack, bulk kelp, rock are with dish and/or black wrack etc. according to algae, spirulina, group, and and for example, described marine alga is bladder wrack and spirulina, and both mass ratios are 2:1.Preferably, described marine alga is bladder wrack, below be mainly described for bladder wrack.Or described marine alga is other kinds.Such as, marine alga 0.4-1.6 part is also comprised.Such as, a kind of biotechnological formulation, it comprises the component of following quality: alfalfa meal 4-5 part, aloe powder 2-3 part, soyflour 2-3 part, marine alga 0.4-1.6 part.Another embodiment is, described biotechnological formulation also comprises seaweed powder 0.5-1.5 part.Such as, the ratio of alfalfa meal, aloe powder and soyflour is 4:3:3, altogether 10g, in water-soluble preparation, the total content of effective constituent is 10%, then water is 90g, adds up to 100g, simplify estimation and be about 100mL, in the ratio of seaweed powder 5-15g/1L, then add seaweed powder 0.5-1.5g; Preferably, the concentration of seaweed powder is 10g/1L, then often liter of liquid formulation adds seaweed powder 1g.
Another embodiment is, alfalfa meal: aloe powder: soyflour is 4:3:3 to 2:1:1.Such as, alfalfa meal is 4 parts to 5 parts, aloe powder is 2 parts to 3 parts, and soyflour is 2 parts to 3 parts, and its ratio belongs to following scope, i.e. alfalfa meal: aloe powder: soyflour is 4:3:3 to 2:1:1, such as, alfalfa meal: aloe powder: soyflour is 4:3:3, has alfalfa meal 4 parts, aloe powder 3 parts, soyflour 3 parts; And for example alfalfa meal: aloe powder: soyflour is 2:1:1, has alfalfa meal 4 parts, aloe powder 2 parts, soyflour 2 parts, or, there is alfalfa meal 5 parts, aloe powder 2.5 parts, soyflour 2.5 parts etc., by that analogy.Such as, a kind of biotechnological formulation, it comprises the component of following quality: alfalfa meal: aloe powder: soyflour is 4:3:3, or, alfalfa meal: aloe powder: soyflour is 3.5:2:1, or, alfalfa meal: aloe powder: soyflour is 3:1.2:1.5, or, alfalfa meal: aloe powder: soyflour is 2:1:1; Preferably, aloe powder: soyflour is 1:1, namely both is identical in quality.
The extraction feed of biotechnological formulation of the present invention also comprises some alternative plant materials, and there is no particular restriction for its kind, can according to season and region characteristic by simple experiment determination add-on.Such as, the alternative plant material that can add comprises wheat germ powder, corn stalk powder, Semen Maydis powder, mealy potato, Powder Phaseoli radiati etc.Such as, its consumption is respectively the 10%-20% of alfalfa meal consumption, and such as, comprise wheat germ powder and corn stalk powder, consumption is respectively the 10%-20% of alfalfa meal consumption.Such as, a kind of biotechnological formulation, it comprises the component of following quality: alfalfa meal 5 parts, aloe powder 2 parts, soyflour 2 parts, Semen Maydis powder 1 part, Powder Phaseoli radiati 0.5 part.And for example, the consumption of alternative plant material is total up to the 10%-20% of alfalfa meal consumption, and such as, comprise Semen Maydis powder and mealy potato, its consumption is total up to the 10%-20% of alfalfa meal consumption.And for example, a kind of biotechnological formulation, it comprises the component of following quality: alfalfa meal 4 parts, aloe powder 2 parts, soyflour 2 parts, Semen Maydis powder 0.4 part, mealy potato 0.4 part; And for example, a kind of biotechnological formulation, it comprises the component of following quality: alfalfa meal 5 parts, aloe powder 2.5 parts, soyflour 2.5 parts, wheat germ powder 0.2 part, corn stalk powder 0.1 part, mealy potato 0.2 part.By that analogy.
Another embodiment is, described biotechnological formulation also comprises water at low temperature 15 to 190 parts.Such as, a kind of biotechnological formulation, it comprises the component of following quality: alfalfa meal, aloe powder and soyflour 4:3:3, altogether 10g, and in preparation, the total content of effective constituent is 5%-40%, and water is 15g-190g.Such as, water at low temperature is temperature lower than the Natural Water of 30 degrees Celsius.And for example, water at low temperature is the Natural Water of temperature in 10 to 30 degree Celsius range, comprises tap water, well water, river, lake water etc.And for example, water at low temperature is the distilled water of temperature in 10 to 30 degree Celsius range.And for example, water at low temperature is the deionized water of temperature in 10 to 30 degree Celsius range.Such as, the temperature of water at low temperature is 5,10,15,20 or 25 degrees Celsius.Another embodiment is, the mixture of alfalfa meal, aloe powder and soyflour adopts the extraction of water at low temperature non-fermented.That is, adopt the present embodiment, need not fermenting process, only water at low temperature need be adopted to extract, avoid the fermentation step that prior art is commonly used.
And for example, soybean lecithin is adopted to substitute soyflour described in above-mentioned any embodiment; Such as, according to the soybean lecithin content in soybean, and the mass ratio of soybean in biotechnological formulation, the mass ratio of soybean lecithin is set; Such as, a kind of pure plant extract, can the biotechnological formulation of comprehensive improvement soil, it comprises alfalfa meal, aloe powder and soybean lecithin, and such as, the soybean lecithin content in soybean is 1% to 5%, then the mass ratio of alfalfa meal, aloe powder and soybean lecithin is 4-5:2-3:0.02-0.15, such as, calculate by soybean 2-3 part, 2*1%=0.02 part, 3*0.05=0.15 part, namely soybean lecithin is 0.02-0.15 part.And for example, in described preparation, the mass ratio of each component is alfalfa meal: aloe powder: soybean lecithin=4 ~ 3:3 ~ 2:0.03 ~ 0.12.And for example, the making method of described preparation is carry out non-fermented extraction by the water at low temperature of less than 30 DEG C.Another example is, finally adds organic marine alga in described preparation, and such as organic marine alga is bladder wrack.
Another embodiment of the present invention is, a kind of preparation method of biotechnological formulation, and it comprises the following steps: alfalfa meal, aloe powder and soyflour are carried out low-temperature extraction by the mass ratio of 4-5:2-3:2-3.Such as, leaching after the mixing of alfalfa meal, aloe powder and soyflour is placed in water at low temperature, and carries out low-temperature ultrasonic extraction treatment.Such as, described low temperature is 5 to 30 degrees Celsius, and adopt ordinary method to carry out ultrasonic extraction process in low temperature, and for example, hyperacoustic frequency is 20-100kHz.Such as, under 25 degrees Celsius of environment, carry out frequency is the process of 50kHz ultrasonic extraction.And for example, after low-temperature extraction, also filter, obtain filtrate.Preferably, after low-temperature extraction, certain hour is placed in filtrate sealing.Such as, described certain hour is greater than 10 days, and and for example, described certain hour is greater than 25 days.Preferably, by filtrate sealing and standing certain hour.Preferably, after filtrate sealing and standing certain hour, seaweed freeze-drying powder is added.Such as, in the ratio of seaweed powder 5-20g/1L, seaweed freeze-drying powder is added.And for example, in the ratio of seaweed powder 5-15g/1L, seaweed freeze-drying powder is added.And for example, described seaweed freeze-drying powder is bladder wrack lyophilized powder.Preferably, after adding seaweed freeze-drying powder, also place for some time.Such as, described for some time is at least 3 days.And for example, described for some time is at least 5 days.Preferably, for some time is placed in sealing.Preferably, sealing and standing for some time.Preferably, after sealing and standing for some time, also filter, get filtrate as described biotechnological formulation.
Such as, as shown in Figure 1, a kind of preparation method of biotechnological formulation, it comprises the following steps: alfalfa meal, aloe powder and soyflour are placed in water at low temperature by the mass ratio leaching of 4-5:2-3:2-3; Carry out low-temperature extraction; Filter; After certain hour is placed in filtrate sealing, add seaweed freeze-drying powder; After for some time is placed in mixed solution sealing, again filter, obtain filtrate, be described biotechnological formulation.
Such as, described preparation method comprises the following steps:
1, the plant materials such as clover, aloe, soybean are carried out lyophilize.
2, the plant material after lyophilize is ground, obtain canebreak material.
3, the leaching of canebreak material is placed in water at low temperature, and carries out low-temperature ultrasonic extraction treatment.Such as, treatment temp is 20-25 degree.
4, low-temperature extraction liquid is carried out filtration treatment, to remove residue.
5, the filtered liquid after extraction is placed in sealed vessel to place 30 days, adds bladder wrack lyophilized powder.
6, after mixed solution being placed 7 days, filtration can obtain the finished product.Such as, for some time is placed in mixed solution sealing, filters and get filtrate as the finished product.
And for example, described preparation method comprises the following steps:
1, each plant material is carried out lyophilize respectively; Wherein, described plant material comprises clover, aloe, soybean; And for example, described plant material also comprises corn, maize straw, potato, mung bean and/or wheat.
2, respectively each plant material is ground, obtain canebreak material.Wherein, described canebreak material comprises alfalfa meal, aloe powder, soyflour; And for example, described canebreak material also comprises Semen Maydis powder, corn stalk powder, mealy potato, Powder Phaseoli radiati and/or wheat-flour.
3, by the mass fraction of alfalfa meal 4-5 part, aloe powder 2-3 part, soyflour 2-3 part, each canebreak material mixing leaching is placed in water at low temperature, and carries out low-temperature ultrasonic extraction treatment.Such as, treatment temp is 20-25 degree.Such as, leaching after each canebreak material mixing is placed in water at low temperature, and for example, canebreak material is placed into sequentially leaching in water at low temperature and puts; And for example, each canebreak material mixing leaching is placed in 15 to 190 parts of water at low temperature.
4, low-temperature extraction liquid is carried out filtration treatment, to remove residue.Adopt ordinary method filtration treatment, the present invention and each embodiment thereof do not do extra restriction to this.Preferably, filtration treatment is carried out at low ambient temperatures.
5, the filtered liquid after extraction is placed in sealed vessel and places certain hour, then add bladder wrack lyophilized powder.Filtered liquid after extraction is placed in sealed vessel place 30 days, adds bladder wrack lyophilized powder.
6, after mixed solution being placed for some time, filtration can obtain described biotechnological formulation.Such as, described for some time is at least 3 days; And for example, after mixed solution being placed 7 days, filtration can obtain the finished product.
Another embodiment is, a kind of preparation method of biotechnological formulation, and it comprises the following steps: for arbitrary above-mentioned biotechnological formulation, and alfalfa meal, aloe powder and soyflour are carried out low-temperature extraction by the mass ratio of 4-5:2-3:2-3.Such as, alfalfa meal, aloe powder and soyflour are pressed 4-5 part: 2-3 part: the mass ratio of 2-3 part, add in 15 to 190 parts of water at low temperature and carry out low-temperature extraction.Such as, described low temperature be temperature lower than 30 degrees Celsius, and for example, described low temperature is that temperature is in 5 to 30 degree Celsius range; And for example, described low temperature is that temperature is in 10 to 30 degree Celsius range.Another embodiment is, alfalfa meal, aloe powder and soyflour is carried out low-temperature extraction by the mass ratio of 4-5:2-3:2-3, adds a small amount of marine alga more afterwards.
Another embodiment is, adds a small amount of marine alga according to the volume of extraction liquid.Such as, freeze-drying seaweed powder is added according to the volume of extraction liquid.And for example, according to the volume of extraction liquid, by the mass volume ratio example of 5-20g/1L, add freeze-drying seaweed powder.Preferably, according to the volume of extraction liquid, the mass volume ratio example of 5-15g/1L, adds freeze-drying seaweed powder.Wherein, described freeze-drying seaweed powder, be the seaweed powder adopting conventional freeze drying method to obtain, the present invention and each related embodiment thereof do not do extra restriction to this.Another embodiment is, described marine alga is bladder wrack.
Another embodiment is, adopts water at low temperature extraction alfalfa meal, aloe powder and soyflour.Such as, water at low temperature is temperature lower than the Natural Water of 30 degrees Celsius.And for example, water at low temperature is the Natural Water of temperature in 10 to 30 degree Celsius range, distilled water or deionized water.
Another embodiment of the present invention is as follows: adopt the biotechnological formulation that described in above-mentioned any embodiment, preparation method obtains.
Another embodiment of the present invention is as follows: the application of arbitrary above-mentioned biotechnological formulation in soil improvement.Another embodiment is, the concentration of described biotechnological formulation is 5%-40%.Another embodiment is, the consumption of described biotechnological formulation is 20-100mL/ mu.Another example is, biotechnological formulation described in above-mentioned any embodiment, the application on degenerated soil.Such as, described degenerated soil for hardening, salinization, the soil such as desertification.
Another embodiment of the present invention is as follows: the application of arbitrary above-mentioned biotechnological formulation in fertilizer.Such as, biotechnological formulation described in above-mentioned any embodiment, the common fertilizer as farm crop is used.Such as, described farm crop are melon and fruit class, Cereal, greengrocery etc.Another embodiment is, described application is applied to the farm crop of melon and fruit class, Cereal or greengrocery.Such as, the concentration of described biotechnological formulation is 5%-40%, for as Term Fertilization.
Described in the present invention and each embodiment thereof, biotechnological formulation provides with finished product preparation form, is diluted to required concentration and sprays to soil and plant blade during use.Such as, because also containing the nutrition fertilizer that some plants can directly absorb in preparation, so spray blade better effects if.
Provide an example of using method below again, it comprise following some.
1, by this biotechnological formulation according to dilution 500-1000 doubly after spray on plants blade and soil.
2, whole crop cycle uses 2-3 time, and when first time uses as transplanting front or transplanting, second time uses as first time uses latter about 3 weeks.
3, consumption is determined according to different crops and edatope, is generally 25mL-50mL/ mu.
As can be seen here, this biotechnological formulation consumption is less, and cost is lower, and suit large area to popularize use.
Continue below in conjunction with embody rule, provide several embodiment.
Embodiment 1, cleans up plant water sources such as the clover of fresh harvesting, aloe, soybean.Thereafter, plant is inserted in freeze drier, freezing with-10 DEG C, and with 2-4 per hour DEG C of cooling, keep 24 hours to remove plant moisture.Plant sample ice bath after freeze-drying is ground to 60 orders, subsequently alfalfa meal, aloe powder and soyflour is added in 30 DEG C of water at low temperature pre-treatment 3 hours in the ratio of 4:3:3; Then pretreated plant is put into low-temperature ultrasonic handler, with the concussion frequency processing 30min of 40KHz, obtain low-temperature extraction liquid.The filtration of low-temperature extraction liquid is placed on normal temperature in sealed vessel and places 30 days, the ratio extracting filtered liquid according to 10g/1L adds bladder wrack lyophilized powder, then places 7 days, and filter, namely obtain this preparation after packaging, being generally brown has special odor liquid.
Embodiment 2, this is the application case in a saltings.
Regional general situation: be experimental field positioned at Shenxian County, Shandong Province, this plot, due to life-time service chemical fertilizer, causes significantly hardening and salinification phenomenon.
For studying thing: sweet corn.
Test sample: the biotechnological formulation prepared by embodiment 1.
Test method: experimental field will be divided into 3 process, comprises one blank group (T1) and two treatment group (T2, T3), each process 3 repetition, amounts to 9 regions.Test site sprays this preparation according to the consumption of 25mL/ mu (T2) and 50mL/ mu (T3) respectively in latter 20 days with transplanting after transplanting, and blank group (T1) then sprays the clear water of identical amount.
Using method: after this reagent dilutions 1000 times, evenly spray on blade and soil with atomizer.
Test index and method: the collection of pedotheque adopts the method for multidraw, hybrid test, chooses root region soil and measures.The mensuration of soil physical properties is with reference to " NYT 1121-2006 Soil K+adsorption ".
Test-results: after a Growing season, the change of the growing state of corn, output and soil physico-chemical property is measured.The each process of corn sample is chosen 20 strains and is carried out measurement and average, and estimates per mu yield.The collection of pedotheque adopts the method for multidraw, hybrid test, chooses root region soil and measures.Concrete test-results is as follows:
(1) corn growth situation and output
Note: tabular data presentation is mean+SD, shows significant difference with lowercase alphabet different after column data.
Find out from upper table, after using this preparation, can effectively promote that milpa increases, significantly improve corn ear length, increase grain number per spike and output.Wherein T2 plant height, spike length, grain number per spike and output add 3.5%, 4.9%, 4.0% and 11.8% than blank respectively; T3 plant height, spike length, grain number per spike and output add 4.0%, 5.7%, 5.2% and 15.2% than blank respectively.This preparation does not have obvious promoter action to Ear Width of Maize.T3 process is slightly better than T2 in corn plant height and output, but difference does not reach conspicuous level.
(2) soil physical properties change
Note: tabular data presentation is mean+SD, shows significant difference with lowercase alphabet different after column data.
Find out from upper table, effectively can reduce the soil weight after using this preparation, increase maximum water holding capacity and total porosity, between T2 and T3, difference is not remarkable.Wherein T2 and T3 unit weight all declines 8% than blank, and water retaining capacity increases by 8.5% and 9.0% respectively than blank respectively, and total porosity increases by 15.1% and 33.7% than blank respectively; Illustrate that this preparation can effectively improve soil compaction, chessom, increase porosity and the water-holding power of soil.
(3) soil chemical property
Find out from upper table, the content of the soil organism and microbial biomass carbon after using this preparation, can be significantly improved, illustrate that this preparation is improved the function of soil fertility; After using this preparation simultaneously, treatment group soil pH compared with blank also closer to neutrality, but due to the time shorter, its difference does not reach conspicuous level.Except microbial biomass carbon content T3 process is significantly than except T2 process height, other both indexs are without significant difference.
Conclusion brief introduction:
1, after using this preparation in corn planting process, its plant strain growth, increase yield can not only be promoted, effectively can also increase sedimentation and the water retention property of soil simultaneously, improve the soil organism and organic C storage, be conducive to preventing Soil degradation and promoting agricultural sustainable development.
2, in consumption, 50mL/ mu is more little than 25mL/ mu difference, and except microbial biomass carbon content 50mL/ mu is higher, other indexs two process is all without significant difference.Therefore for avoiding waste, the consumption of recommendering folder preparation is 25mL/ mu.
Further, embodiments of the invention also comprise, each technical characteristic of the various embodiments described above, biotechnological formulation be mutually combined to form and preparation method thereof and the application in soil improvement, fertilizer.Adopt above-mentioned biotechnological formulation and application thereof, achieve the biotechnological formulation by stimulating endogenous microbes to carry out comprehensive improvement soil, biological soil amendment, belongs to soil remediation and agricultural biological technical field category.After using this biotechnological formulation, the formation of soil aggregate can be promoted, overcome the problem such as soil compaction, salinification caused because using chemical fertilizer in a large number year after year, rebuild the edatope of balance, recover biological activity of soil, improve crop yield and quality.
The biotechnological formulation product adopting formula of the present invention and method to produce and application thereof, tool has the following advantages:
1, the present invention and each embodiment production process thereof adopt low-temperature extraction mode, make the activeconstituents in starting material obtain intact preservation.
2, the present invention and each embodiment thereof do not add any inoculating microbe, therefore can not produce the problems such as bacterial classification variation, decay in production process.
3, the present invention and each embodiment thereof are applied to soil and can effectively activate soil inside source beneficial microorganism, can not cause mutual antagonism antagonistic action between invasive plants, microorganism, break the bad phenomenon such as soil ecology balance.
4, the present invention and each embodiment wide accommodation thereof, use safety, validity period is long, can work, and lasts is long for most of soil and plant.
5, the present invention and each embodiment thereof are used for newly transplanting plant, are conducive to plant fixing and take root.
6, the present invention and each embodiment thereof are applied to agriculture field soil, effectively can increase utilization rate of fertilizer, improve soil organic matter content, reduce soil-borne disease, increase agricultural output and quality.
7, the present invention and each embodiment thereof are applied to harden, salinization, the problematic soils such as barren, can effective chessom, increase soil fertility, promote the formation of soil aggregate and humic acids.
It should be noted that, preferred embodiment of the present invention is given in specification sheets of the present invention and accompanying drawing thereof, but, the present invention can be realized by many different forms, be not limited to the embodiment described by this specification sheets, these embodiments not as the extra restriction to content of the present invention, provide the object of these embodiments be make the understanding of disclosure of the present invention more comprehensively thorough.Further, above-mentioned each technical characteristic continues combination mutually, is formed not in above-named various embodiment, is all considered as the scope that specification sheets of the present invention is recorded; Further, for those of ordinary skills, can be improved according to the above description or convert, and all these improve and convert the protection domain that all should belong to claims of the present invention.
Claims (10)
1. a biotechnological formulation, is characterized in that, comprises the component of following quality:
Alfalfa meal 4-5 part,
Aloe powder 2-3 part,
Soyflour 2-3 part.
2. biotechnological formulation according to claim 1, is characterized in that, alfalfa meal: aloe powder: soyflour is 4:3:3 to 2:1:1.
3. biotechnological formulation according to claim 1, is characterized in that, also comprise water at low temperature 15 to 190 parts.
4. biotechnological formulation according to claim 1, is characterized in that, also comprise seaweed powder 0.5-1.5 part.
5. biotechnological formulation according to claim 4, it is characterized in that, described marine alga is brown alga.
6. biotechnological formulation according to claim 5, it is characterized in that, described marine alga is bladder wrack.
7. a preparation method for biotechnological formulation, is characterized in that, comprises the following steps: alfalfa meal, aloe powder and soyflour are carried out low-temperature extraction by the mass ratio of 4-5:2-3:2-3.
8. the application of biotechnological formulation in soil improvement as described in as arbitrary in claim 1 to 6.
9. the application of biotechnological formulation in fertilizer as described in as arbitrary in claim 1 to 6.
10. apply according to claim 9, it is characterized in that, be applied to the farm crop of melon and fruit class, Cereal or greengrocery.
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