CN104755931B - 用于检测细胞样品中的活细胞的方法 - Google Patents

用于检测细胞样品中的活细胞的方法 Download PDF

Info

Publication number
CN104755931B
CN104755931B CN201380035732.8A CN201380035732A CN104755931B CN 104755931 B CN104755931 B CN 104755931B CN 201380035732 A CN201380035732 A CN 201380035732A CN 104755931 B CN104755931 B CN 104755931B
Authority
CN
China
Prior art keywords
membrane
cells
fluorescent
quencher
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201380035732.8A
Other languages
English (en)
Chinese (zh)
Other versions
CN104755931A (zh
Inventor
诺尔曼·R·韦恩赖特
巴拉·S·曼尼亚
埃里克·斯廷普森
布瑞恩·J·科洛尼亚
罗伯特·K·科洛尼亚
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Li Meiteli Ces Co Ltd
Charles River Laboratories Inc
Original Assignee
Li Meiteli Ces Co Ltd
Charles River Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Li Meiteli Ces Co Ltd, Charles River Laboratories Inc filed Critical Li Meiteli Ces Co Ltd
Publication of CN104755931A publication Critical patent/CN104755931A/zh
Application granted granted Critical
Publication of CN104755931B publication Critical patent/CN104755931B/zh
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN201380035732.8A 2012-05-02 2013-05-02 用于检测细胞样品中的活细胞的方法 Expired - Fee Related CN104755931B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261641809P 2012-05-02 2012-05-02
US61/641,809 2012-05-02
US201361784789P 2013-03-14 2013-03-14
US61/784,789 2013-03-14
PCT/US2013/039349 WO2013166337A1 (en) 2012-05-02 2013-05-02 Method of detecting viable cells in a cell sample

Publications (2)

Publication Number Publication Date
CN104755931A CN104755931A (zh) 2015-07-01
CN104755931B true CN104755931B (zh) 2016-08-31

Family

ID=48670053

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201380035732.8A Expired - Fee Related CN104755931B (zh) 2012-05-02 2013-05-02 用于检测细胞样品中的活细胞的方法

Country Status (5)

Country Link
US (2) US9709500B2 (https=)
EP (1) EP2780707B1 (https=)
JP (1) JP6482459B2 (https=)
CN (1) CN104755931B (https=)
WO (1) WO2013166337A1 (https=)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2013256174B2 (en) 2012-05-02 2017-03-02 Charles River Laboratories, Inc. Viability staining method
EP2769204B1 (en) 2012-05-02 2016-02-17 Charles River Laboratories, Inc. Cell capture system and use thereof
EP2780707B1 (en) 2012-05-02 2016-02-24 Charles River Laboratories, Inc. Method of detecting viable cells in a cell sample
USD717460S1 (en) * 2013-03-14 2014-11-11 Reametrix, Inc. Cell detection system
USD719667S1 (en) * 2013-03-14 2014-12-16 Reametrix, Inc. Cell detection system
USD719666S1 (en) * 2013-03-14 2014-12-16 Reametrix, Inc. Cell detection system with touchscreen
WO2014153369A1 (en) * 2013-03-18 2014-09-25 Life Technologies Corporation Methods and systems for analyzing biological reaction systems
WO2015066727A1 (en) * 2013-11-04 2015-05-07 Charles River Laboratories, Inc. Method for detecting viable cells in a cell sample
USD724755S1 (en) 2013-11-04 2015-03-17 Charles River Laboratories, Inc. Cup
CN105874052B (zh) * 2013-11-04 2019-05-21 查尔斯河实验室公司 过滤系统和其使用
US20150218612A1 (en) * 2014-02-06 2015-08-06 Tacount Exact Ltd. Apparatus, system and method for live bacteria microscopy
US9436866B2 (en) 2014-08-01 2016-09-06 General Electric Company High sensitivity flat panel microbiology detection and enumeration system
USD782694S1 (en) 2014-11-04 2017-03-28 Charles River Laboratories, Inc. Filtration device
USD776295S1 (en) 2014-11-04 2017-01-10 Charles River Laboratories, Inc. Base
USD776296S1 (en) 2014-11-04 2017-01-10 Charles River Laboratories, Inc. Adapter
USD759836S1 (en) 2014-11-04 2016-06-21 Charles River Laboratories, Inc. Cup
WO2016172532A2 (en) * 2015-04-23 2016-10-27 Bd Kiestra B.V. A method and system for automated microbial colony counting from streaked sample on plated media
USD817509S1 (en) * 2016-10-14 2018-05-08 Spartan Bioscience Inc. Diagnostic device
JP2018183088A (ja) * 2017-04-26 2018-11-22 アズビル株式会社 細胞生存率判定装置
CA3075267C (en) * 2017-09-07 2021-01-26 Eagle Analytical Services, Inc. Process and system for flow cytometry fluorescent detection of reactive materials in viscous non-filterable materials
GB201716883D0 (en) 2017-10-13 2017-11-29 Q-Linea Ab Method for determining the concentration of intact microorganisms in a sample
US10564100B1 (en) * 2018-10-12 2020-02-18 SageMedic Corporation Analysis of viable and nonviable cells
WO2020106788A1 (en) 2018-11-21 2020-05-28 Cornell University Optical clearing and auto-fluorescence quenching solutions and method of use for enhanced microscopy imaging of biological tissues
US20220081671A1 (en) * 2018-12-21 2022-03-17 Global Life Sciences Solutions Usa Llc In-Process Device and Method for Cell Culture Monitoring
KR102205473B1 (ko) * 2018-12-21 2021-01-21 칸트사이언스 주식회사 세포 내 pH 측정방법
DE102019004870B4 (de) 2019-07-11 2023-03-09 Particle Metrix Gmbh Vorrichtung und Verfahren zur Reduzierung der Intensitätsminderung des Fluoreszenzfarbstoffes durch Laserlicht bei der Bestimmung der Fluoreszenz und der Anzahl von Antikörpern auf Exosomen.
US20230294097A1 (en) * 2020-05-04 2023-09-21 The Regents Of The University Of California Electrochemical cellular circuits
CN115684100A (zh) * 2021-07-28 2023-02-03 上海睿钰生物科技有限公司 一种检测细胞样品的特征参数的方法
TWI866690B (zh) * 2023-02-07 2024-12-11 邦睿生技股份有限公司 生物樣品檢測裝置

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992002632A1 (en) * 1990-07-30 1992-02-20 Sierra Cytometry Fluorescent dyes for identification and enumeration of viable cells in milk
EP0713087A1 (en) * 1994-11-17 1996-05-22 Chemunex Apparatus and process for rapid and ultrasensitive detection and counting of microorganisms by fluorescence
CN1582394A (zh) * 2001-09-06 2005-02-16 基因描绘系统有限公司 分子的快速灵敏检测
WO2010006615A2 (en) * 2008-07-14 2010-01-21 Chemometec A/S Method and kit for assessing viable cells

Family Cites Families (122)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1256360A (en) 1984-02-10 1989-06-27 John W. Bornhoeft Apparatus and method for determining the effectiveness of sterlization
US4561553A (en) 1985-01-22 1985-12-31 Northern Engineering And Plastics Corp. Snap on twist off tamper-proof closure for containers
EP0215066A1 (en) 1985-02-27 1987-03-25 University Of Cincinnati Viable microorganism detection by induced fluorescence
US4739416A (en) 1986-09-15 1988-04-19 Matrix Instruments Inc. Digital image reproduction
US4725891A (en) 1986-09-15 1988-02-16 Matrix Instruments Inc. Film image digitizer
US4783401A (en) 1986-10-31 1988-11-08 Smithkline Beckman Corporation Viable cell labelling
US5037656A (en) 1986-12-04 1991-08-06 Millipore Corporation Porous membrane having hydrophilic and cell growth promotions surface and process
JPH0813930B2 (ja) 1987-12-28 1996-02-14 三井東圧化学株式会社 近赤外線吸収フィルター用高純度アントラキノン系色素
US4930893A (en) 1988-03-25 1990-06-05 Molecular Dynamics Electrophoresis imaging system
US4838632A (en) 1988-05-06 1989-06-13 Lumisys Inc. Two-dimensional beam scanner
US5073488A (en) 1988-11-29 1991-12-17 Minnesota Mining And Manufacturing Company Rapid method for determining efficacy of a sterilization cycle and rapid read-out biological indicator
US5185450A (en) 1991-02-19 1993-02-09 University Of South Florida Tetrazolium compounds for cell viability assays
US5172419A (en) 1991-03-05 1992-12-15 Lumisys, Inc. Medical image processing system
US5296341A (en) 1991-03-05 1994-03-22 Lumisys, Inc. Green light laser imaging method
US5314805A (en) 1991-10-28 1994-05-24 Molecular Probes, Inc. Dual-fluorescence cell viability assay using ethidium homodimer and calcein AM
US5234585A (en) 1991-12-19 1993-08-10 Zuk, Incorporated Vacuum filtration device
US5843680A (en) 1992-01-31 1998-12-01 Biometric Imaging, Inc. Differential separation assay methods and test kits
US5137609A (en) 1992-01-31 1992-08-11 Biometric Imaging Inc. Differential separation assay
US5221454A (en) 1992-01-31 1993-06-22 Biometric Imaging Inc. Differential separation assay
DE69311944T2 (de) 1992-04-01 1997-11-20 Nihon Millipore Kogyo K K Verfahren zur Bestimmung der Anzahl lebender Mikroorganismen
JP3228812B2 (ja) 1993-02-10 2001-11-12 日本マイクロリス株式会社 生菌数を測定する方法
US5547849A (en) 1993-02-17 1996-08-20 Biometric Imaging, Inc. Apparatus and method for volumetric capillary cytometry
US5585246A (en) 1993-02-17 1996-12-17 Biometric Imaging, Inc. Method for preparing a sample in a scan capillary for immunofluorescent interrogation
US5658751A (en) 1993-04-13 1997-08-19 Molecular Probes, Inc. Substituted unsymmetrical cyanine dyes with selected permeability
US5545535A (en) 1993-04-13 1996-08-13 Molecular Probes, Inc. Fluorescent assay for bacterial gram reaction
US5436134A (en) 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
US5534416A (en) 1993-04-13 1996-07-09 Molecular Probes, Inc. Fluorescent viability assay using cyclic-substituted unsymmetrical cyanine dyes
US5445946A (en) 1993-04-13 1995-08-29 Molecular Probes, Inc. Intravacuolar stains for yeast and other fungi
US5437980A (en) 1993-05-17 1995-08-01 Molecular Probes, Inc. Phenanthridium dye staining of nucleic acids in living cells
FR2707665A1 (fr) 1993-06-28 1995-01-20 Chemunex Procédé de mesure et d'évaluation de la vitalité des microorganismes et ses applications.
JP3011036B2 (ja) 1993-12-20 2000-02-21 三菱化学株式会社 光ディスク基板の複屈折の測定法
IES940182A2 (en) * 1994-03-01 1995-11-29 Teagasc Agric Food Dev Authori "Rapid detection of bacteria in liquid cultures"
JP3137160B2 (ja) 1994-04-01 2001-02-19 日立電子エンジニアリング株式会社 ウエハの位置ズレ補正方法
US5550032A (en) 1994-05-27 1996-08-27 George Mason University Biological assay for microbial contamination
JP3593161B2 (ja) 1994-11-15 2004-11-24 株式会社トプコン 回転体上異物位置測定装置
US5603900A (en) 1995-05-19 1997-02-18 Millipore Investment Holdings Limited Vacuum filter device
US5565678A (en) 1995-06-06 1996-10-15 Lumisys, Inc. Radiographic image quality assessment utilizing a stepped calibration target
WO1997012226A1 (en) 1995-09-25 1997-04-03 Tencor Instruments Improved system for surface inspection
HUP9902028A2 (hu) 1995-11-08 1999-11-29 Pioneer Hi-Bred International, Inc. Eljárások mikrobatenyészetek életképességének becslésére
EP0881489A1 (en) 1995-12-29 1998-12-02 Wako Pure Chemical Industries Ltd Method for determining viable cell count
US6459805B1 (en) 1996-03-26 2002-10-01 Childrens Hospital Los Angeles Fluorescence digital imaging microscopy system
DE19621312A1 (de) 1996-05-28 1997-12-04 Bayer Ag Maskierung der Hintergrundfluoreszenz und Signalverstärkung bei der optischen Analyse biologisch medizinischer Assays
US5956146A (en) 1997-01-29 1999-09-21 Victor Company Of Japan, Ltd. Birefringence measuring apparatus for optical disc substrate
US6221621B1 (en) 1997-03-06 2001-04-24 Bard Diagnostic Sciences, Inc. Methods of screening for colorectal cancers in which a complement Factor I or related protein is associated
FR2764305B1 (fr) 1997-06-04 2000-10-06 Chemunex Procede de detection et de numeration de cellules viables dans un echantillon biologique et kit pour sa mise en oeuvre
US6200762B1 (en) 1997-08-01 2001-03-13 Aurora Biosciences Corporation Photon reducing agents and compositions for fluorescence assays
US6214563B1 (en) 1997-08-01 2001-04-10 Aurora Biosciences Corporation Photon reducing agents for reducing undesired light emission in assays
US6221612B1 (en) 1997-08-01 2001-04-24 Aurora Biosciences Corporation Photon reducing agents for use in fluorescence assays
US6043506A (en) 1997-08-13 2000-03-28 Bio-Rad Laboratories, Inc. Multi parameter scanner
US5922617A (en) 1997-11-12 1999-07-13 Functional Genetics, Inc. Rapid screening assay methods and devices
CN1230682C (zh) 1997-12-30 2005-12-07 埃佩多夫阵列技术股份有限公司 探测和量化目标分子的方法及探测和读取装置
US6080868A (en) 1998-01-23 2000-06-27 The Perkin-Elmer Corporation Nitro-substituted non-fluorescent asymmetric cyanine dye compounds
EP1101106A4 (en) 1998-07-28 2006-05-03 Bd Biosciences Systems And Rea ARRANGEMENT AND METHOD FOR THE TEST FOR CELL MOBILITY
US6130745A (en) 1999-01-07 2000-10-10 Biometric Imaging, Inc. Optical autofocus for use with microtiter plates
JP2000232897A (ja) * 1999-02-15 2000-08-29 Nippon Mizushori Giken:Kk 菌類の即時判別方法
DE60002562T2 (de) 1999-02-26 2004-04-15 Cellomics, Inc. Ein system für zellbasierte reihenuntersuchungen
JP2000304688A (ja) 1999-04-16 2000-11-02 Canon Inc 基板測定方法および装置
US6181413B1 (en) 1999-09-03 2001-01-30 Biometric Imaging, Inc. Displacing volume in field of view
AU4134801A (en) 1999-10-25 2001-05-30 Genprime, Inc. Method and apparatus for prokaryotic and eukaryotic cell quantitation
JP2001228088A (ja) 2000-02-18 2001-08-24 Nippon Laser & Electronics Lab 生体試料光学的走査装置
CA2337987A1 (en) 2000-02-25 2001-08-25 Elmex Limited Membrane filtration system
JP2001242082A (ja) 2000-02-29 2001-09-07 Nippon Laser & Electronics Lab 生体試料光学的走査装置
GB0008787D0 (en) 2000-04-10 2000-05-31 Norsk Naeringsmiddelforskning A method of cell detection
US6323337B1 (en) 2000-05-12 2001-11-27 Molecular Probes, Inc. Quenching oligonucleotides
US7118878B1 (en) 2000-06-09 2006-10-10 Promega Corporation Method for increasing luminescence assay sensitivity
IL139593A (en) 2000-11-09 2010-12-30 Biogem Optical Ltd Method for the detection of viable microorganisms
US7546925B1 (en) 2000-12-04 2009-06-16 Roush Life Sciences, Llc Disposable vacuum filtration apparatus capable of detecting microorganisms and particulates in liquid samples
US6714287B2 (en) 2001-01-02 2004-03-30 Becton, Dickinson And Company Apparatus for determining the volume of single red blood cells
US6633368B2 (en) 2001-01-02 2003-10-14 Becton, Dickinson And Company Method for determining the volume of single red blood cells
EP1364207A4 (en) 2001-01-25 2005-02-09 Thaco Res Ltd FAST PROCEDURES FOR MICROBIAL TYPING AND COUNTING
JP2002310886A (ja) 2001-04-11 2002-10-23 Canon Inc ディスクサイトメトリーによる分析方法及び装置
US7016087B2 (en) 2001-08-08 2006-03-21 Becton Dickinson And Company Photon efficient scanner
US6750457B2 (en) 2001-08-29 2004-06-15 Becton Dickinson And Company System for high throughput analysis
US7205100B2 (en) 2002-10-10 2007-04-17 Advanced Analytical Technologies, Inc. Methods for reducing background fluorescence
JP4231920B2 (ja) * 2003-08-01 2009-03-04 独立行政法人産業技術総合研究所 ディスク状バイオチップ及びその読取り装置
US7435576B2 (en) 2003-09-30 2008-10-14 Gen-Probe Incorporated Filter snapper
ES2239886B1 (es) 2003-11-05 2006-12-16 Universidad De Barcelona Metodo y aparato para la determinacion de la viabilidad celular.
EP1699351B1 (en) 2003-12-22 2011-05-04 Lightouch Medical, Inc. Process for determination of cell viability
WO2005088287A1 (en) 2004-03-17 2005-09-22 National Research Council Of Canada Method and apparatus for the detection of microorganisms
DE102004019685A1 (de) 2004-04-20 2005-11-17 DRäGER AEROSPACE GMBH Passagiersauerstoffmaske
KR20070061802A (ko) 2004-07-29 2007-06-14 킴 래버러터리즈, 아이엔씨. 초음파 감응 센서 및 분석 대상물의 고속 검출 방법
US20060129327A1 (en) 2004-07-29 2006-06-15 Kim Myung L Ultrasensitive sensor and rapid detection of analytes
EP1624071B1 (en) 2004-08-06 2008-10-22 Fuji Electric Holdings Co., Ltd. Method of detecting viable cells
JP4779433B2 (ja) 2004-08-26 2011-09-28 パナソニック株式会社 ろ過フィルタデバイス
US7018804B1 (en) 2004-12-03 2006-03-28 Orion Biosolutions, Inc. Determination of cell viability and phenotype
US20060121443A1 (en) 2004-12-03 2006-06-08 Orion Biosolutions, Inc. Determination of cell viability
EP1853387A4 (en) 2005-02-22 2009-11-11 Univ Cincinnati DETERMINATION OF LIVABLE MICROORGANISMS BY COATED PARAMAGNETIC BEADS
JP2007033170A (ja) * 2005-07-26 2007-02-08 Fujifilm Corp 蛍光検出方法および蛍光検出システム
WO2007028157A1 (en) 2005-09-02 2007-03-08 Zuk Peter Jr Systems, apparatus and methods for vacuum filtration
CA2623597A1 (en) 2005-09-16 2007-03-16 The Charles Stark Draper Laboratory, Inc. Reaction sensing in living cells
US8364409B2 (en) 2006-01-12 2013-01-29 Biosense Technologies, Inc. Method and composition for rapid viability testing of cells
DE102006022877B4 (de) 2006-05-15 2009-01-29 Sartorius Stedim Biotech Gmbh Verfahren zur Bestimmung der Viabilität von Zellen in Zellkulturen
GB0615302D0 (en) 2006-08-01 2006-09-13 Biotrace Internat Plc Assay Method For The Detection Of Viable Microbial Cells In A Sample
US20080153125A1 (en) 2006-11-10 2008-06-26 Advanced Analytical Technologies, Inc. Rapid detection of microorganisms in fluids
CN101201312A (zh) 2006-12-15 2008-06-18 北京林业大学 一种中性红染色法检测藻细胞活性的方法
EP2142665B1 (en) 2007-04-13 2013-11-27 Promega Corporation Luminescent live and dead cell assay
US20080305514A1 (en) * 2007-06-06 2008-12-11 Alcon Research, Ltd. Method for detecting microbes
WO2009029039A1 (en) 2007-08-28 2009-03-05 Ridgeview Instruments Ab Method for measurement of cell viability
US20090099525A1 (en) 2007-10-11 2009-04-16 Animas Corporation Drug Delivery System with Breakaway Plunger Extractor
MX2010005079A (es) 2007-11-09 2010-05-20 Genzyme Corp Metodos para medir la viabilidad celular sin usar celulas de control.
WO2009082218A1 (en) 2007-12-20 2009-07-02 Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno Real-time method for the detection of viable micro-organisms
US20110110861A1 (en) 2008-05-13 2011-05-12 Mireille Hanna Lahoud Methods of Detecting Cells with a Disrupted Cell Membrane, Cells Infected with A Pathogen, Dying Cells or Dead Cells
CN101344476B (zh) 2008-08-20 2011-03-16 吉林农业大学 细菌活的非可培养状态荧光显微镜观察与计数方法
ES2540544T3 (es) 2009-05-04 2015-07-10 Novartis Ag Microensayo rápido de esterilidad
FR2945457B1 (fr) 2009-05-12 2011-07-15 Aes Chemunex Ensemble forme d'une membrane de filtration et d'une plaque de support, ainsi que dispositif de test microbiologique en faisant usage.
US8148515B1 (en) 2009-06-02 2012-04-03 Biotium, Inc. Detection using a dye and a dye modifier
WO2010151131A2 (en) 2009-06-26 2010-12-29 Nederlandse Organisatie Voor Toegepast- Natuurwetenschappelijk Onderzoek Tno Apparatus for detecting viable microorganisms or spores in a sample and use thereof.
SG177738A1 (en) 2009-07-24 2012-02-28 Morinaga Milk Industry Co Ltd Method and kit for detecting microorganisms
CA2781516C (en) 2010-03-26 2016-01-26 Morinaga Milk Industry Co., Ltd. Culture medium for specific growth, detection, and enumeration of bifidobacterium breve
GB201005939D0 (en) 2010-04-09 2010-05-26 Biostatus Ltd Method of analysing a cell or other biological material containing nucleic acid
US20110294206A1 (en) * 2010-05-28 2011-12-01 California Institute Of Technology Methods and design of membrane filters
JP5717846B2 (ja) 2010-06-03 2015-05-13 ビーエーエスエフ ソシエタス・ヨーロピアＢａｓｆ Ｓｅ 微生物の検出及び定量化
WO2012051437A1 (en) 2010-10-13 2012-04-19 Life Technologies Corporation Compositions and assays for determining cell viability
WO2012059785A1 (en) 2010-11-03 2012-05-10 Reametrix Inc. Methods for calibrating a raman spectrometer, and spectrometer
CA2815951A1 (en) 2010-11-03 2012-05-10 Reametrix Inc. Measurement system for fluorescent detection, and method therefor
CA2816014A1 (en) 2010-11-03 2012-05-10 Reametrix Inc. Method and device for fluorescent measurement of samples
EP2637787A1 (en) 2010-11-08 2013-09-18 Reactrix Systems, Inc. Sample assembly for a measurement device
US9446411B2 (en) 2011-02-24 2016-09-20 Reametrix, Inc. Sample assembly for a measurement device
US20130068310A1 (en) 2011-09-15 2013-03-21 University Of Washington Through Its Center Of Commercialization Method and Apparatus for a Microfluidic Device
EP2769204B1 (en) 2012-05-02 2016-02-17 Charles River Laboratories, Inc. Cell capture system and use thereof
AU2013256174B2 (en) 2012-05-02 2017-03-02 Charles River Laboratories, Inc. Viability staining method
EP2780707B1 (en) 2012-05-02 2016-02-24 Charles River Laboratories, Inc. Method of detecting viable cells in a cell sample

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992002632A1 (en) * 1990-07-30 1992-02-20 Sierra Cytometry Fluorescent dyes for identification and enumeration of viable cells in milk
EP0713087A1 (en) * 1994-11-17 1996-05-22 Chemunex Apparatus and process for rapid and ultrasensitive detection and counting of microorganisms by fluorescence
CN1582394A (zh) * 2001-09-06 2005-02-16 基因描绘系统有限公司 分子的快速灵敏检测
WO2010006615A2 (en) * 2008-07-14 2010-01-21 Chemometec A/S Method and kit for assessing viable cells

Also Published As

Publication number Publication date
EP2780707B1 (en) 2016-02-24
US9709500B2 (en) 2017-07-18
US20130323745A1 (en) 2013-12-05
US20180120231A1 (en) 2018-05-03
JP6482459B2 (ja) 2019-03-13
JP2015517656A (ja) 2015-06-22
WO2013166337A1 (en) 2013-11-07
EP2780707A1 (en) 2014-09-24
CN104755931A (zh) 2015-07-01

Similar Documents

Publication Publication Date Title
CN104755931B (zh) 用于检测细胞样品中的活细胞的方法
CN104662425B (zh) 活性染色方法
CN104685346B (zh) 细胞捕获系统及其使用
RU2517618C2 (ru) Способ и система для определения количества культивируемых клеток
CA2459320A1 (en) Rapid detection of replicating cells
US10883134B2 (en) Method for detecting, identifying, or counting microorganisms, and system using same
CN101432739A (zh) 超灵敏传感器和分析物的快速检测
JP4830103B2 (ja) 生理活性判定方法、及び生理活性判定用キット
US20160298162A1 (en) Method for detecting viable cells in a cell sample
US20180348131A1 (en) Method and apparatus for rapid detection of bacterial contamination
Yeo et al. The Applications of Responsive Fluorescent Sensors to Biological Systems
Zhuang et al. Microbial Imaging with AIE-Active Materials

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20160831