CN104755931B - 用于检测细胞样品中的活细胞的方法 - Google Patents

用于检测细胞样品中的活细胞的方法 Download PDF

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Publication number
CN104755931B
CN104755931B CN201380035732.8A CN201380035732A CN104755931B CN 104755931 B CN104755931 B CN 104755931B CN 201380035732 A CN201380035732 A CN 201380035732A CN 104755931 B CN104755931 B CN 104755931B
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Prior art keywords
membrane
cells
fluorescent
quencher
cell
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Expired - Fee Related
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CN201380035732.8A
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English (en)
Chinese (zh)
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CN104755931A (zh
Inventor
诺尔曼·R·韦恩赖特
巴拉·S·曼尼亚
埃里克·斯廷普森
布瑞恩·J·科洛尼亚
罗伯特·K·科洛尼亚
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Li Meiteli Ces Co Ltd
Charles River Laboratories Inc
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Li Meiteli Ces Co Ltd
Charles River Laboratories Inc
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Publication of CN104755931A publication Critical patent/CN104755931A/zh
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN201380035732.8A 2012-05-02 2013-05-02 用于检测细胞样品中的活细胞的方法 Expired - Fee Related CN104755931B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261641809P 2012-05-02 2012-05-02
US61/641,809 2012-05-02
US201361784789P 2013-03-14 2013-03-14
US61/784,789 2013-03-14
PCT/US2013/039349 WO2013166337A1 (en) 2012-05-02 2013-05-02 Method of detecting viable cells in a cell sample

Publications (2)

Publication Number Publication Date
CN104755931A CN104755931A (zh) 2015-07-01
CN104755931B true CN104755931B (zh) 2016-08-31

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CN201380035732.8A Expired - Fee Related CN104755931B (zh) 2012-05-02 2013-05-02 用于检测细胞样品中的活细胞的方法

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Country Link
US (2) US9709500B2 (https=)
EP (1) EP2780707B1 (https=)
JP (1) JP6482459B2 (https=)
CN (1) CN104755931B (https=)
WO (1) WO2013166337A1 (https=)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2872149C (en) 2012-05-02 2019-03-19 Charles River Laboratories, Inc. Viability staining method
CN104755931B (zh) 2012-05-02 2016-08-31 查尔斯河实验室公司 用于检测细胞样品中的活细胞的方法
CN104685346B (zh) 2012-05-02 2018-05-29 查尔斯河实验室公司 细胞捕获系统及其使用
USD717460S1 (en) * 2013-03-14 2014-11-11 Reametrix, Inc. Cell detection system
USD719667S1 (en) * 2013-03-14 2014-12-16 Reametrix, Inc. Cell detection system
USD719666S1 (en) * 2013-03-14 2014-12-16 Reametrix, Inc. Cell detection system with touchscreen
US9898820B2 (en) * 2013-03-18 2018-02-20 Life Technologies Corporation Methods and systems for analyzing biological reaction systems
USD724755S1 (en) 2013-11-04 2015-03-17 Charles River Laboratories, Inc. Cup
JP6556155B2 (ja) 2013-11-04 2019-08-07 チャールズ リバー ラボラトリーズ， インコーポレイテッド ろ過システム及びその用途
WO2015066727A1 (en) * 2013-11-04 2015-05-07 Charles River Laboratories, Inc. Method for detecting viable cells in a cell sample
US20150218612A1 (en) * 2014-02-06 2015-08-06 Tacount Exact Ltd. Apparatus, system and method for live bacteria microscopy
US9436866B2 (en) 2014-08-01 2016-09-06 General Electric Company High sensitivity flat panel microbiology detection and enumeration system
USD776296S1 (en) 2014-11-04 2017-01-10 Charles River Laboratories, Inc. Adapter
USD776295S1 (en) 2014-11-04 2017-01-10 Charles River Laboratories, Inc. Base
USD759836S1 (en) 2014-11-04 2016-06-21 Charles River Laboratories, Inc. Cup
USD782694S1 (en) 2014-11-04 2017-03-28 Charles River Laboratories, Inc. Filtration device
EP3286732B1 (en) * 2015-04-23 2025-09-10 BD Kiestra B.V. Method and system for automatically counting microbial colonies
USD817509S1 (en) * 2016-10-14 2018-05-08 Spartan Bioscience Inc. Diagnostic device
JP2018183088A (ja) * 2017-04-26 2018-11-22 アズビル株式会社 細胞生存率判定装置
CA3075267C (en) * 2017-09-07 2021-01-26 Eagle Analytical Services, Inc. Process and system for flow cytometry fluorescent detection of reactive materials in viscous non-filterable materials
GB201716883D0 (en) * 2017-10-13 2017-11-29 Q-Linea Ab Method for determining the concentration of intact microorganisms in a sample
US10564100B1 (en) 2018-10-12 2020-02-18 SageMedic Corporation Analysis of viable and nonviable cells
US11994451B2 (en) 2018-11-21 2024-05-28 Cornell University Optical clearing and auto-fluorescence quenching solutions and method of use for enhanced microscopy imaging of biological tissues
CN113196286A (zh) * 2018-12-21 2021-07-30 环球生命科技咨询美国有限责任公司 用于细胞培养监测的过程中装置和方法
KR102205473B1 (ko) * 2018-12-21 2021-01-21 칸트사이언스 주식회사 세포 내 pH 측정방법
DE102019004870B4 (de) 2019-07-11 2023-03-09 Particle Metrix Gmbh Vorrichtung und Verfahren zur Reduzierung der Intensitätsminderung des Fluoreszenzfarbstoffes durch Laserlicht bei der Bestimmung der Fluoreszenz und der Anzahl von Antikörpern auf Exosomen.
US20230294097A1 (en) * 2020-05-04 2023-09-21 The Regents Of The University Of California Electrochemical cellular circuits
CN113567408A (zh) * 2021-07-28 2021-10-29 上海睿钰生物科技有限公司 一种确定荧光通道曝光时间的方法
TWI866690B (zh) * 2023-02-07 2024-12-11 邦睿生技股份有限公司 生物樣品檢測裝置

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992002632A1 (en) * 1990-07-30 1992-02-20 Sierra Cytometry Fluorescent dyes for identification and enumeration of viable cells in milk
EP0713087A1 (en) * 1994-11-17 1996-05-22 Chemunex Apparatus and process for rapid and ultrasensitive detection and counting of microorganisms by fluorescence
CN1582394A (zh) * 2001-09-06 2005-02-16 基因描绘系统有限公司 分子的快速灵敏检测
WO2010006615A2 (en) * 2008-07-14 2010-01-21 Chemometec A/S Method and kit for assessing viable cells

Family Cites Families (122)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1256360A (en) 1984-02-10 1989-06-27 John W. Bornhoeft Apparatus and method for determining the effectiveness of sterlization
US4561553A (en) 1985-01-22 1985-12-31 Northern Engineering And Plastics Corp. Snap on twist off tamper-proof closure for containers
EP0215066A1 (en) 1985-02-27 1987-03-25 University Of Cincinnati Viable microorganism detection by induced fluorescence
US4739416A (en) 1986-09-15 1988-04-19 Matrix Instruments Inc. Digital image reproduction
US4725891A (en) 1986-09-15 1988-02-16 Matrix Instruments Inc. Film image digitizer
US4783401A (en) 1986-10-31 1988-11-08 Smithkline Beckman Corporation Viable cell labelling
US5037656A (en) 1986-12-04 1991-08-06 Millipore Corporation Porous membrane having hydrophilic and cell growth promotions surface and process
JPH0813930B2 (ja) 1987-12-28 1996-02-14 三井東圧化学株式会社 近赤外線吸収フィルター用高純度アントラキノン系色素
US4930893A (en) 1988-03-25 1990-06-05 Molecular Dynamics Electrophoresis imaging system
US4838632A (en) 1988-05-06 1989-06-13 Lumisys Inc. Two-dimensional beam scanner
US5073488A (en) 1988-11-29 1991-12-17 Minnesota Mining And Manufacturing Company Rapid method for determining efficacy of a sterilization cycle and rapid read-out biological indicator
US5185450A (en) 1991-02-19 1993-02-09 University Of South Florida Tetrazolium compounds for cell viability assays
US5296341A (en) 1991-03-05 1994-03-22 Lumisys, Inc. Green light laser imaging method
US5172419A (en) 1991-03-05 1992-12-15 Lumisys, Inc. Medical image processing system
US5314805A (en) 1991-10-28 1994-05-24 Molecular Probes, Inc. Dual-fluorescence cell viability assay using ethidium homodimer and calcein AM
US5234585A (en) 1991-12-19 1993-08-10 Zuk, Incorporated Vacuum filtration device
US5843680A (en) 1992-01-31 1998-12-01 Biometric Imaging, Inc. Differential separation assay methods and test kits
US5137609A (en) 1992-01-31 1992-08-11 Biometric Imaging Inc. Differential separation assay
US5221454A (en) 1992-01-31 1993-06-22 Biometric Imaging Inc. Differential separation assay
DE563858T1 (de) 1992-04-01 1994-03-03 Nihon Millipore Kogyo K K Verfahren zur Bestimmung der Anzahl lebender Mikroorganismen.
JP3228812B2 (ja) 1993-02-10 2001-11-12 日本マイクロリス株式会社 生菌数を測定する方法
US5547849A (en) 1993-02-17 1996-08-20 Biometric Imaging, Inc. Apparatus and method for volumetric capillary cytometry
US5585246A (en) 1993-02-17 1996-12-17 Biometric Imaging, Inc. Method for preparing a sample in a scan capillary for immunofluorescent interrogation
US5436134A (en) 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
US5534416A (en) 1993-04-13 1996-07-09 Molecular Probes, Inc. Fluorescent viability assay using cyclic-substituted unsymmetrical cyanine dyes
US5658751A (en) 1993-04-13 1997-08-19 Molecular Probes, Inc. Substituted unsymmetrical cyanine dyes with selected permeability
US5445946A (en) 1993-04-13 1995-08-29 Molecular Probes, Inc. Intravacuolar stains for yeast and other fungi
US5545535A (en) 1993-04-13 1996-08-13 Molecular Probes, Inc. Fluorescent assay for bacterial gram reaction
US5437980A (en) 1993-05-17 1995-08-01 Molecular Probes, Inc. Phenanthridium dye staining of nucleic acids in living cells
FR2707665A1 (fr) 1993-06-28 1995-01-20 Chemunex Procédé de mesure et d'évaluation de la vitalité des microorganismes et ses applications.
JP3011036B2 (ja) 1993-12-20 2000-02-21 三菱化学株式会社 光ディスク基板の複屈折の測定法
IES940182A2 (en) * 1994-03-01 1995-11-29 Teagasc Agric Food Dev Authori "Rapid detection of bacteria in liquid cultures"
JP3137160B2 (ja) 1994-04-01 2001-02-19 日立電子エンジニアリング株式会社 ウエハの位置ズレ補正方法
US5550032A (en) 1994-05-27 1996-08-27 George Mason University Biological assay for microbial contamination
JP3593161B2 (ja) 1994-11-15 2004-11-24 株式会社トプコン 回転体上異物位置測定装置
US5603900A (en) 1995-05-19 1997-02-18 Millipore Investment Holdings Limited Vacuum filter device
US5565678A (en) 1995-06-06 1996-10-15 Lumisys, Inc. Radiographic image quality assessment utilizing a stepped calibration target
WO1997012226A1 (en) 1995-09-25 1997-04-03 Tencor Instruments Improved system for surface inspection
AU7603096A (en) 1995-11-08 1997-05-29 Pioneer Hi-Bred International, Inc. Methods of assessing viability of microbial cultures
US20020119438A1 (en) 1995-12-29 2002-08-29 Fuminori Kato Method for determining viable cell count
US6459805B1 (en) 1996-03-26 2002-10-01 Childrens Hospital Los Angeles Fluorescence digital imaging microscopy system
DE19621312A1 (de) 1996-05-28 1997-12-04 Bayer Ag Maskierung der Hintergrundfluoreszenz und Signalverstärkung bei der optischen Analyse biologisch medizinischer Assays
US5956146A (en) 1997-01-29 1999-09-21 Victor Company Of Japan, Ltd. Birefringence measuring apparatus for optical disc substrate
US6221621B1 (en) 1997-03-06 2001-04-24 Bard Diagnostic Sciences, Inc. Methods of screening for colorectal cancers in which a complement Factor I or related protein is associated
FR2764305B1 (fr) 1997-06-04 2000-10-06 Chemunex Procede de detection et de numeration de cellules viables dans un echantillon biologique et kit pour sa mise en oeuvre
US6221612B1 (en) 1997-08-01 2001-04-24 Aurora Biosciences Corporation Photon reducing agents for use in fluorescence assays
US6214563B1 (en) 1997-08-01 2001-04-10 Aurora Biosciences Corporation Photon reducing agents for reducing undesired light emission in assays
US6200762B1 (en) 1997-08-01 2001-03-13 Aurora Biosciences Corporation Photon reducing agents and compositions for fluorescence assays
US6043506A (en) 1997-08-13 2000-03-28 Bio-Rad Laboratories, Inc. Multi parameter scanner
US5922617A (en) 1997-11-12 1999-07-13 Functional Genetics, Inc. Rapid screening assay methods and devices
EP1044375B1 (en) 1997-12-30 2005-12-14 Remacle, José Method comprising capture molecule fixed on disc surface
US6080868A (en) 1998-01-23 2000-06-27 The Perkin-Elmer Corporation Nitro-substituted non-fluorescent asymmetric cyanine dye compounds
JP2002521690A (ja) 1998-07-28 2002-07-16 バイオメトリック イメージング インコーポレイテッド 細胞運動性アッセイのための装置および方法
US6130745A (en) 1999-01-07 2000-10-10 Biometric Imaging, Inc. Optical autofocus for use with microtiter plates
JP2000232897A (ja) * 1999-02-15 2000-08-29 Nippon Mizushori Giken:Kk 菌類の即時判別方法
ATE239907T1 (de) 1999-02-26 2003-05-15 Cellomics Inc Ein system für zellbasierte reihenuntersuchungen
JP2000304688A (ja) 1999-04-16 2000-11-02 Canon Inc 基板測定方法および装置
US6181413B1 (en) 1999-09-03 2001-01-30 Biometric Imaging, Inc. Displacing volume in field of view
ATE399878T1 (de) 1999-10-25 2008-07-15 Genprime Inc Verfahren und vorrichtung zur quantifizierung von prokaryotischen und eukaryotischen zellen
JP2001228088A (ja) 2000-02-18 2001-08-24 Nippon Laser & Electronics Lab 生体試料光学的走査装置
CA2337987A1 (en) 2000-02-25 2001-08-25 Elmex Limited Membrane filtration system
JP2001242082A (ja) 2000-02-29 2001-09-07 Nippon Laser & Electronics Lab 生体試料光学的走査装置
GB0008787D0 (en) 2000-04-10 2000-05-31 Norsk Naeringsmiddelforskning A method of cell detection
US6323337B1 (en) 2000-05-12 2001-11-27 Molecular Probes, Inc. Quenching oligonucleotides
US7118878B1 (en) 2000-06-09 2006-10-10 Promega Corporation Method for increasing luminescence assay sensitivity
IL139593A (en) 2000-11-09 2010-12-30 Biogem Optical Ltd Method for the detection of viable microorganisms
US7546925B1 (en) 2000-12-04 2009-06-16 Roush Life Sciences, Llc Disposable vacuum filtration apparatus capable of detecting microorganisms and particulates in liquid samples
US6714287B2 (en) 2001-01-02 2004-03-30 Becton, Dickinson And Company Apparatus for determining the volume of single red blood cells
US6633368B2 (en) 2001-01-02 2003-10-14 Becton, Dickinson And Company Method for determining the volume of single red blood cells
US20020098531A1 (en) 2001-01-25 2002-07-25 Thacker James D. Rapid methods for microbial typing and enumeration
JP2002310886A (ja) 2001-04-11 2002-10-23 Canon Inc ディスクサイトメトリーによる分析方法及び装置
US7016087B2 (en) 2001-08-08 2006-03-21 Becton Dickinson And Company Photon efficient scanner
US6750457B2 (en) 2001-08-29 2004-06-15 Becton Dickinson And Company System for high throughput analysis
US7205100B2 (en) 2002-10-10 2007-04-17 Advanced Analytical Technologies, Inc. Methods for reducing background fluorescence
JP4231920B2 (ja) * 2003-08-01 2009-03-04 独立行政法人産業技術総合研究所 ディスク状バイオチップ及びその読取り装置
US7435576B2 (en) 2003-09-30 2008-10-14 Gen-Probe Incorporated Filter snapper
ES2239886B1 (es) 2003-11-05 2006-12-16 Universidad De Barcelona Metodo y aparato para la determinacion de la viabilidad celular.
ATE507765T1 (de) 2003-12-22 2011-05-15 Lightouch Medical Inc Verfahren zur bestimmung der lebensfähigkeit von zellen
EP1728069A4 (en) 2004-03-17 2009-08-19 Ca Nat Research Council METHOD AND DEVICE FOR DETECTING MICROORGANISMS
DE102004019685A1 (de) 2004-04-20 2005-11-17 DRäGER AEROSPACE GMBH Passagiersauerstoffmaske
US20060129327A1 (en) 2004-07-29 2006-06-15 Kim Myung L Ultrasensitive sensor and rapid detection of analytes
KR20070061802A (ko) 2004-07-29 2007-06-14 킴 래버러터리즈, 아이엔씨. 초음파 감응 센서 및 분석 대상물의 고속 검출 방법
DE602005010515D1 (de) 2004-08-06 2008-12-04 Fuji Electric Holdings Nachweisverfahren für lebenfähige Zellen
JP4779433B2 (ja) 2004-08-26 2011-09-28 パナソニック株式会社 ろ過フィルタデバイス
US7018804B1 (en) 2004-12-03 2006-03-28 Orion Biosolutions, Inc. Determination of cell viability and phenotype
US20060121443A1 (en) 2004-12-03 2006-06-08 Orion Biosolutions, Inc. Determination of cell viability
WO2006091630A2 (en) 2005-02-22 2006-08-31 University Of Cincinnati Determination of viable microorganisms using coated paramagnetic beads
JP2007033170A (ja) * 2005-07-26 2007-02-08 Fujifilm Corp 蛍光検出方法および蛍光検出システム
WO2007028157A1 (en) 2005-09-02 2007-03-08 Zuk Peter Jr Systems, apparatus and methods for vacuum filtration
CA2623597A1 (en) 2005-09-16 2007-03-16 The Charles Stark Draper Laboratory, Inc. Reaction sensing in living cells
EP1981983B1 (en) 2006-01-12 2015-02-18 Biosense Technologies, Inc. Method and composition for rapid viability testing of cells
DE102006022877B4 (de) 2006-05-15 2009-01-29 Sartorius Stedim Biotech Gmbh Verfahren zur Bestimmung der Viabilität von Zellen in Zellkulturen
GB0615302D0 (en) 2006-08-01 2006-09-13 Biotrace Internat Plc Assay Method For The Detection Of Viable Microbial Cells In A Sample
WO2008130445A2 (en) 2006-11-10 2008-10-30 Advanced Analytical Technologies, Inc. Rapid detection microorganisms in fluids
CN101201312A (zh) 2006-12-15 2008-06-18 北京林业大学 一种中性红染色法检测藻细胞活性的方法
AU2008239654A1 (en) 2007-04-13 2008-10-23 Promega Corporation Luminescent live and dead cell assay
US20080305514A1 (en) 2007-06-06 2008-12-11 Alcon Research, Ltd. Method for detecting microbes
WO2009029039A1 (en) 2007-08-28 2009-03-05 Ridgeview Instruments Ab Method for measurement of cell viability
US20090099525A1 (en) 2007-10-11 2009-04-16 Animas Corporation Drug Delivery System with Breakaway Plunger Extractor
ES2498674T3 (es) 2007-11-09 2014-09-25 Genzyme Corporation Métodos para medir la viabilidad celular sin usar células de control
WO2009082218A1 (en) 2007-12-20 2009-07-02 Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno Real-time method for the detection of viable micro-organisms
US20110110861A1 (en) 2008-05-13 2011-05-12 Mireille Hanna Lahoud Methods of Detecting Cells with a Disrupted Cell Membrane, Cells Infected with A Pathogen, Dying Cells or Dead Cells
CN101344476B (zh) 2008-08-20 2011-03-16 吉林农业大学 细菌活的非可培养状态荧光显微镜观察与计数方法
BRPI1013966A2 (pt) 2009-05-04 2016-08-09 Novartis Ag microensaio de esterilidade rápida.
FR2945457B1 (fr) 2009-05-12 2011-07-15 Aes Chemunex Ensemble forme d'une membrane de filtration et d'une plaque de support, ainsi que dispositif de test microbiologique en faisant usage.
US8148515B1 (en) 2009-06-02 2012-04-03 Biotium, Inc. Detection using a dye and a dye modifier
WO2010151131A2 (en) 2009-06-26 2010-12-29 Nederlandse Organisatie Voor Toegepast- Natuurwetenschappelijk Onderzoek Tno Apparatus for detecting viable microorganisms or spores in a sample and use thereof.
KR101383389B1 (ko) 2009-07-24 2014-04-08 모리나가 뉴교 가부시키가이샤 미생물 검출법 및 미생물 검출 키트
EP2554677B1 (en) 2010-03-26 2015-04-29 Morinaga Milk Industry Co., Ltd. Viable bacteria count measurement method and culture medium
GB201005939D0 (en) 2010-04-09 2010-05-26 Biostatus Ltd Method of analysing a cell or other biological material containing nucleic acid
US20110294206A1 (en) * 2010-05-28 2011-12-01 California Institute Of Technology Methods and design of membrane filters
AU2011262289B2 (en) 2010-06-03 2014-03-20 Basf Se Detection and enumeration of microorganisms
WO2012051437A1 (en) 2010-10-13 2012-04-19 Life Technologies Corporation Compositions and assays for determining cell viability
CA2815951A1 (en) 2010-11-03 2012-05-10 Reametrix Inc. Measurement system for fluorescent detection, and method therefor
CA2816014A1 (en) 2010-11-03 2012-05-10 Reametrix Inc. Method and device for fluorescent measurement of samples
WO2012059785A1 (en) 2010-11-03 2012-05-10 Reametrix Inc. Methods for calibrating a raman spectrometer, and spectrometer
WO2012063097A1 (en) 2010-11-08 2012-05-18 Reametrix Inc. Sample assembly for a measurement device
US9446411B2 (en) 2011-02-24 2016-09-20 Reametrix, Inc. Sample assembly for a measurement device
US20130068310A1 (en) 2011-09-15 2013-03-21 University Of Washington Through Its Center Of Commercialization Method and Apparatus for a Microfluidic Device
CN104755931B (zh) 2012-05-02 2016-08-31 查尔斯河实验室公司 用于检测细胞样品中的活细胞的方法
CN104685346B (zh) 2012-05-02 2018-05-29 查尔斯河实验室公司 细胞捕获系统及其使用
CA2872149C (en) 2012-05-02 2019-03-19 Charles River Laboratories, Inc. Viability staining method

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992002632A1 (en) * 1990-07-30 1992-02-20 Sierra Cytometry Fluorescent dyes for identification and enumeration of viable cells in milk
EP0713087A1 (en) * 1994-11-17 1996-05-22 Chemunex Apparatus and process for rapid and ultrasensitive detection and counting of microorganisms by fluorescence
CN1582394A (zh) * 2001-09-06 2005-02-16 基因描绘系统有限公司 分子的快速灵敏检测
WO2010006615A2 (en) * 2008-07-14 2010-01-21 Chemometec A/S Method and kit for assessing viable cells

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