CN104651487B - 检测食源性致病菌的试剂盒及多重荧光pcr检测方法 - Google Patents
检测食源性致病菌的试剂盒及多重荧光pcr检测方法 Download PDFInfo
- Publication number
- CN104651487B CN104651487B CN201410621337.4A CN201410621337A CN104651487B CN 104651487 B CN104651487 B CN 104651487B CN 201410621337 A CN201410621337 A CN 201410621337A CN 104651487 B CN104651487 B CN 104651487B
- Authority
- CN
- China
- Prior art keywords
- target gene
- food
- borne pathogens
- primers
- downstream
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 244000078673 foodborn pathogen Species 0.000 title claims abstract description 43
- 238000001514 detection method Methods 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 claims abstract description 32
- 238000012408 PCR amplification Methods 0.000 claims abstract description 20
- 238000004458 analytical method Methods 0.000 claims abstract description 15
- 238000006243 chemical reaction Methods 0.000 claims abstract description 9
- 239000007850 fluorescent dye Substances 0.000 claims abstract description 9
- 108090000623 proteins and genes Proteins 0.000 claims description 57
- 108020004465 16S ribosomal RNA Proteins 0.000 claims description 46
- 241000607142 Salmonella Species 0.000 claims description 32
- 241000191967 Staphylococcus aureus Species 0.000 claims description 21
- 241000607272 Vibrio parahaemolyticus Species 0.000 claims description 20
- 241000186779 Listeria monocytogenes Species 0.000 claims description 19
- 238000004519 manufacturing process Methods 0.000 claims description 11
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 8
- 101100504328 Escherichia coli O157:H7 perA gene Proteins 0.000 claims description 8
- 238000013461 design Methods 0.000 claims description 8
- 101150004681 rfbE gene Proteins 0.000 claims description 8
- 102100034213 ATPase family protein 2 homolog Human genes 0.000 claims description 7
- 108010091193 spermatogenesis associated factor Proteins 0.000 claims description 7
- 238000000545 stagnation point adsorption reflectometry Methods 0.000 claims description 7
- 210000001072 colon Anatomy 0.000 claims description 6
- 238000004925 denaturation Methods 0.000 claims description 6
- 230000036425 denaturation Effects 0.000 claims description 6
- 238000003753 real-time PCR Methods 0.000 claims description 6
- 244000052769 pathogen Species 0.000 claims description 5
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 claims description 4
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 claims description 4
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 claims description 4
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 claims description 4
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 claims description 4
- 238000002844 melting Methods 0.000 claims description 4
- 230000008018 melting Effects 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 230000001717 pathogenic effect Effects 0.000 claims description 4
- 230000004044 response Effects 0.000 claims description 4
- 238000000137 annealing Methods 0.000 claims description 3
- 230000007850 degeneration Effects 0.000 claims description 3
- 238000007499 fusion processing Methods 0.000 claims description 3
- 238000003752 polymerase chain reaction Methods 0.000 claims description 3
- 238000004153 renaturation Methods 0.000 claims description 3
- 230000003287 optical effect Effects 0.000 claims 1
- 230000003321 amplification Effects 0.000 abstract description 6
- 238000003199 nucleic acid amplification method Methods 0.000 abstract description 6
- 238000012360 testing method Methods 0.000 abstract description 6
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 3
- 239000000284 extract Substances 0.000 abstract description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 2
- 108010006785 Taq Polymerase Proteins 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 239000000047 product Substances 0.000 description 20
- 108020004414 DNA Proteins 0.000 description 18
- 239000000523 sample Substances 0.000 description 12
- 235000013305 food Nutrition 0.000 description 11
- 241000607598 Vibrio Species 0.000 description 9
- 241000894006 Bacteria Species 0.000 description 8
- 239000000975 dye Substances 0.000 description 8
- 241000186781 Listeria Species 0.000 description 7
- 206010047400 Vibrio infections Diseases 0.000 description 6
- 208000015181 infectious disease Diseases 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 241000191940 Staphylococcus Species 0.000 description 5
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 4
- 241001646719 Escherichia coli O157:H7 Species 0.000 description 4
- 206010018910 Haemolysis Diseases 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 4
- 241000231286 Neottia Species 0.000 description 4
- 244000052616 bacterial pathogen Species 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 230000008588 hemolysis Effects 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 239000013612 plasmid Substances 0.000 description 4
- 241000251468 Actinopterygii Species 0.000 description 3
- 241000193468 Clostridium perfringens Species 0.000 description 3
- 241001478240 Coccus Species 0.000 description 3
- 241000305071 Enterobacterales Species 0.000 description 3
- 208000005374 Poisoning Diseases 0.000 description 3
- 206010039438 Salmonella Infections Diseases 0.000 description 3
- 235000009754 Vitis X bourquina Nutrition 0.000 description 3
- 235000012333 Vitis X labruscana Nutrition 0.000 description 3
- 240000006365 Vitis vinifera Species 0.000 description 3
- 235000014787 Vitis vinifera Nutrition 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 235000013601 eggs Nutrition 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 235000019688 fish Nutrition 0.000 description 3
- 231100000572 poisoning Toxicity 0.000 description 3
- 230000000607 poisoning effect Effects 0.000 description 3
- 206010039447 salmonellosis Diseases 0.000 description 3
- 238000012795 verification Methods 0.000 description 3
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 2
- 241001333951 Escherichia coli O157 Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 2
- 241000589776 Pseudomonas putida Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 241000607734 Yersinia <bacteria> Species 0.000 description 2
- 230000001174 ascending effect Effects 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 210000003608 fece Anatomy 0.000 description 2
- 230000002949 hemolytic effect Effects 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- PHTQWCKDNZKARW-UHFFFAOYSA-N isoamylol Chemical compound CC(C)CCO PHTQWCKDNZKARW-UHFFFAOYSA-N 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 235000013336 milk Nutrition 0.000 description 2
- 239000008267 milk Substances 0.000 description 2
- 210000004080 milk Anatomy 0.000 description 2
- 244000144977 poultry Species 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 208000004998 Abdominal Pain Diseases 0.000 description 1
- 241001474374 Blennius Species 0.000 description 1
- 241000589875 Campylobacter jejuni Species 0.000 description 1
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 1
- 241000588923 Citrobacter Species 0.000 description 1
- 241000588919 Citrobacter freundii Species 0.000 description 1
- 241000938605 Crocodylia Species 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 108010067770 Endopeptidase K Proteins 0.000 description 1
- 241000588914 Enterobacter Species 0.000 description 1
- 241000588921 Enterobacteriaceae Species 0.000 description 1
- 241000194033 Enterococcus Species 0.000 description 1
- 241000194032 Enterococcus faecalis Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000192125 Firmicutes Species 0.000 description 1
- 206010016952 Food poisoning Diseases 0.000 description 1
- 208000019331 Foodborne disease Diseases 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 206010061598 Immunodeficiency Diseases 0.000 description 1
- 241000588748 Klebsiella Species 0.000 description 1
- 241000186805 Listeria innocua Species 0.000 description 1
- 201000009906 Meningitis Diseases 0.000 description 1
- 206010027906 Monocytosis Diseases 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 238000002944 PCR assay Methods 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 241000588769 Proteus <enterobacteria> Species 0.000 description 1
- 241000588767 Proteus vulgaris Species 0.000 description 1
- 238000012181 QIAquick gel extraction kit Methods 0.000 description 1
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 1
- 206010040047 Sepsis Diseases 0.000 description 1
- 241000607768 Shigella Species 0.000 description 1
- 241000607766 Shigella boydii Species 0.000 description 1
- 239000007984 Tris EDTA buffer Substances 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- 241000607447 Yersinia enterocolitica Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 238000005452 bending Methods 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 230000001010 compromised effect Effects 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 238000007821 culture assay Methods 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 229940032049 enterococcus faecalis Drugs 0.000 description 1
- 231100000655 enterotoxin Toxicity 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000021393 food security Nutrition 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 244000053095 fungal pathogen Species 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000005802 health problem Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 235000015110 jellies Nutrition 0.000 description 1
- 239000008274 jelly Substances 0.000 description 1
- 239000010985 leather Substances 0.000 description 1
- 235000021190 leftovers Nutrition 0.000 description 1
- 238000007726 management method Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 235000013622 meat product Nutrition 0.000 description 1
- 238000011880 melting curve analysis Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 210000003928 nasal cavity Anatomy 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 235000013594 poultry meat Nutrition 0.000 description 1
- 235000013613 poultry product Nutrition 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 229940007042 proteus vulgaris Drugs 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 235000014102 seafood Nutrition 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 208000013223 septicemia Diseases 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 201000008827 tuberculosis Diseases 0.000 description 1
- 230000008673 vomiting Effects 0.000 description 1
- 229940098232 yersinia enterocolitica Drugs 0.000 description 1
- 206010048282 zoonosis Diseases 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
参考菌株 | 编号 | 结果 |
Salmonella.choleraesuis(猪霍乱沙门氏菌) | ATCC 10708 | + |
Vibrio.parahaemolyticus(副溶血性弧菌) | ATCC 17802 | + |
Salmonella.Enteritidis(肠炎沙门氏菌) | ATCC 13076 | + |
Salmonella.Typhimurium(鼠伤寒沙门氏菌) | ATCC 13311 | + |
staphylococcus.aureus(金黄色葡萄球菌) | ATCC 25923 | + |
staphylococcus.aureus(金黄色葡萄球菌) | ATCC 49444 | + |
Vibrio Parahemolyticus(副溶血性弧菌) | ATCC 17802 | + |
Listeria.monocytogenes(单核细胞增生李斯特氏菌) | ATCC 19114 | + |
Escherchia coli(大肠埃希氏菌O157:H7) | ATCC 43895 | + |
Enterobacter.aerogenes(产气肠杆菌) | ATCC 13048 | - |
Proteus.vulgaris(普通变形菌) | ATCC 6380 | - |
Listeria.grayi(格氏利斯特氏菌) | ATCC 25401 | - |
Listeria.ivanovii(伊氏利斯特氏菌) | ATCC 19615 | - |
Listeria.welshimeri(威氏利斯特氏菌) | ATCC 35897 | - |
Listeria.innocua(英诺克李斯特氏菌) | ATCC 33090 | - |
Listeria.Seeligeri(斯氏利斯特氏菌) | ATCC 35967 | - |
Enterococcus.faecalis(粪肠球菌) | ATCC 29212 | - |
Citrobacter.freundii(弗氏柠檬酸杆菌) | ATCC 8090 | - |
staphylococcus.epidermidis(表皮葡萄球菌) | ATCC 12228 | - |
Klebsiella.pneumoniae(肺炎克雷伯氏菌) | ATCC 4352 | - |
Yersinia.enterocolitica(小肠结肠炎耶尔森氏菌) | ATCC 27729 | - |
Yersinia.Kristensenii(克氏耶尔森氏菌) | ATCC 33639 | - |
Shigella boydii(鲍氏志贺氏菌) | AB 200052 | - |
Campylobacter jejuni(弯曲肠杆菌) | ATCC 33291 | - |
Pseudomonas aeruginosa(铜绿假单胞菌菌) | ATCC 27853 | - |
Pseudomonas putida(恶臭假单胞菌) | ATCC 49128 | - |
Clostridium perfringens(产气荚膜梭菌) | ATCC 13124 | - |
Claims (4)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410621337.4A CN104651487B (zh) | 2014-11-05 | 2014-11-05 | 检测食源性致病菌的试剂盒及多重荧光pcr检测方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410621337.4A CN104651487B (zh) | 2014-11-05 | 2014-11-05 | 检测食源性致病菌的试剂盒及多重荧光pcr检测方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN104651487A CN104651487A (zh) | 2015-05-27 |
CN104651487B true CN104651487B (zh) | 2018-06-15 |
Family
ID=53243126
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201410621337.4A Active CN104651487B (zh) | 2014-11-05 | 2014-11-05 | 检测食源性致病菌的试剂盒及多重荧光pcr检测方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN104651487B (zh) |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104894281A (zh) * | 2015-06-25 | 2015-09-09 | 蔡先全 | 检测转基因大豆mon87708的引物、试剂盒和方法 |
CN104894282A (zh) * | 2015-06-25 | 2015-09-09 | 蔡先全 | 检测转基因玉米cbh351的引物、试剂盒和方法 |
CN104894283A (zh) * | 2015-06-25 | 2015-09-09 | 蔡先全 | 检测沙门氏菌及整合子int的引物、试剂盒及方法 |
CN105256048B (zh) * | 2015-11-06 | 2020-07-21 | 厦门基科生物科技有限公司 | 口腔致病菌多重pcr检测引物组和探针组及其应用 |
CN106011297A (zh) * | 2016-07-30 | 2016-10-12 | 天津市农业质量标准与检测技术研究所 | 一种基于实时荧光pcr技术快速筛查黄瓜中病原微生物的方法 |
CN106916894A (zh) * | 2017-04-06 | 2017-07-04 | 吉林大学 | 一种多重pcr检测微生态活菌制剂中食源性致病菌的方法 |
CN107190079B (zh) * | 2017-06-30 | 2020-04-17 | 北京百康芯生物科技有限公司 | 五种食源性病原菌微流控芯片快速检测技术及试剂盒 |
CN107841570A (zh) * | 2017-12-29 | 2018-03-27 | 福建出入境检验检疫局检验检疫技术中心 | 一种检测沙门菌和居泉沙雷菌的双重实时荧光定量pcr方法 |
CN108486258A (zh) * | 2018-01-29 | 2018-09-04 | 南昌大学 | 一种可视快速检测食源性致病菌的试剂盒及其方法 |
CN108410968A (zh) * | 2018-03-16 | 2018-08-17 | 芜湖市食品药品检验中心(市药品不良反应监测中心) | 餐饮食品中食源性致病菌的快速鉴定方法 |
CN111187828B (zh) * | 2020-02-11 | 2023-09-15 | 圣湘生物科技股份有限公司 | 检测人叶酸代谢基因多态性的组合物、试剂盒及方法 |
CN112538544B (zh) * | 2020-12-30 | 2022-06-14 | 广东省微生物研究所(广东省微生物分析检测中心) | 具有特异性分子靶标的食源性致病菌标准菌株活菌的检测方法及应用 |
CN113151515A (zh) * | 2021-03-31 | 2021-07-23 | 广东产品质量监督检验研究院(国家质量技术监督局广州电气安全检验所、广东省试验认证研究院、华安实验室) | 一种食源性致病菌的检测试剂盒及检测方法 |
CN114381536B (zh) * | 2022-01-21 | 2024-05-17 | 江西省检验检测认证总院食品检验检测研究院 | 五种食源性致病菌的多重pcr检测引物组、试剂盒和方法 |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103725796B (zh) * | 2013-10-29 | 2016-04-20 | 新疆农业大学 | 一种牛病毒性腹泻病毒内标分型荧光pcr检测试剂盒及其制备 |
-
2014
- 2014-11-05 CN CN201410621337.4A patent/CN104651487B/zh active Active
Also Published As
Publication number | Publication date |
---|---|
CN104651487A (zh) | 2015-05-27 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN104651487B (zh) | 检测食源性致病菌的试剂盒及多重荧光pcr检测方法 | |
CN102146469B (zh) | 副溶血性弧菌双重实时荧光pcr检测引物、探针、检测试剂盒和检测方法 | |
Yu et al. | Survey of five food-borne pathogens in commercial cold food dishes and their detection by multiplex PCR | |
Domesle et al. | Validation of a Salmonella loop-mediated isothermal amplification assay in animal food | |
Hong et al. | Quantification and differentiation of Campylobacter jejuni and Campylobacter coli in raw chicken meats using a real-time PCR method | |
CN101705281B (zh) | 检测沙门氏菌的实时荧光pcr试剂盒及检测方法 | |
CN102851385B (zh) | 利用lamp检测溶藻弧菌的引物组及其快速诊断试剂盒 | |
CN109554449B (zh) | 一种可同时检测气单胞菌7个毒力基因的多重pcr方法 | |
CN102140512A (zh) | 致病性嗜水气单胞菌的lamp检测试剂盒及检测方法 | |
Murugadas et al. | Distribution of pathotypes of Escherichia coli in seafood from retail markets of Kerala, India | |
Chapela et al. | Development of a multiplex real-time PCR method for early diagnosis of three bacterial diseases in fish: a real-case study in trout aquaculture | |
CN107365869B (zh) | 利用环介导等温扩增技术检测食源性肺炎克雷伯菌的方法及引物 | |
CN102643919B (zh) | 一种食品中副溶血弧菌活菌的检测试剂盒及检测方法 | |
CN102676664A (zh) | 同时检测多种水产品致病菌的荧光定量pcr引物和探针及检测方法 | |
CN108034740B (zh) | 用于检测印第安纳沙门菌的引物组、该引物组的用途、包含该引物组的试剂盒及其检测方法 | |
CN101319249A (zh) | 阪崎肠杆菌快速检测试剂盒及其检测方法 | |
CN107385077B (zh) | 用于检测鸡白痢沙门氏菌的特异性引物、含有该引物的试剂盒及其应用 | |
CN103184279B (zh) | 非标记荧光pcr结合hrma检测副溶血弧菌的方法 | |
CN111154901B (zh) | 副溶血性弧菌特异性新分子靶标及其快速检测方法 | |
CN103911433A (zh) | 嗜水气单胞菌的lamp检测引物组及试剂盒 | |
CN104651518A (zh) | 一种海豚链球菌环介导等温扩增试剂盒及其应用 | |
CN107022601B (zh) | 用于检测具有国家限量标准的5种食品致病菌的引物组合及其应用 | |
CN103923977B (zh) | 温和气单胞菌的lamp检测引物组及试剂盒 | |
CN104164510A (zh) | 利用多重pcr技术对食源性致病菌进行高通量快速检测的方法 | |
CN103981270A (zh) | 美人鱼发光杆菌快速检测引物、试剂盒及应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB03 | Change of inventor or designer information | ||
CB03 | Change of inventor or designer information |
Inventor after: Cai Xianquan Inventor after: Wu Bing Inventor after: Qiu Deyi Inventor after: Liu Gongyuan Inventor after: Bai Jianshan Inventor after: Yue Qiaoyun Inventor after: Zhao Meizhuan Inventor after: Xiao Qiqian Inventor after: Zhang Lei Inventor before: Cai Xianquan Inventor before: Li Rong Inventor before: Qiu Deyi Inventor before: Yu Haiqiong Inventor before: Bai Jianshan Inventor before: Yue Qiaoyun Inventor before: Zhao Meizhuan Inventor before: Xiao Qiqian Inventor before: Zhang Lei |
|
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20201215 Address after: 246001 e-commerce Industrial Park, NO.48 Renmin Road, Yingjiang District, Anqing City, Anhui Province Patentee after: Wang Zhao Address before: Room 1504, No. 2, Zhongshan Sixth Road, Zhongshan City, Guangdong Province Patentee before: Cai Xianquan |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20210802 Address after: 530000 house on the 12th floor of Taiping financial building, No. 16, Songxiang Road, Nanning area, China (Guangxi) pilot Free Trade Zone, Nanning, Guangxi Zhuang Autonomous Region Patentee after: Guangxi Institute of biological manufacturing technology Co.,Ltd. Address before: 246001 e-commerce Industrial Park, NO.48 Renmin Road, Yingjiang District, Anqing City, Anhui Province Patentee before: Wang Zhao |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Kit for detection of foodborne pathogens and multiplex fluorescent PCR detection method Effective date of registration: 20221215 Granted publication date: 20180615 Pledgee: Nanning Branch of China Everbright Bank Co.,Ltd. Pledgor: Guangxi Institute of biological manufacturing technology Co.,Ltd. Registration number: Y2022450000229 |
|
PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Granted publication date: 20180615 Pledgee: Nanning Branch of China Everbright Bank Co.,Ltd. Pledgor: Guangxi Institute of biological manufacturing technology Co.,Ltd. Registration number: Y2022450000229 |