CN104569443A - 一种Rh血型检测卡 - Google Patents
一种Rh血型检测卡 Download PDFInfo
- Publication number
- CN104569443A CN104569443A CN201410801586.1A CN201410801586A CN104569443A CN 104569443 A CN104569443 A CN 104569443A CN 201410801586 A CN201410801586 A CN 201410801586A CN 104569443 A CN104569443 A CN 104569443A
- Authority
- CN
- China
- Prior art keywords
- gel
- dilution
- monoclonal antibody
- working fluid
- sephadex
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000004369 blood Anatomy 0.000 title claims abstract description 27
- 239000008280 blood Substances 0.000 title claims abstract description 27
- 229920005654 Sephadex Polymers 0.000 claims abstract description 56
- 238000005406 washing Methods 0.000 claims abstract description 21
- 239000008213 purified water Substances 0.000 claims abstract description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 19
- 230000008961 swelling Effects 0.000 claims abstract description 17
- 239000003085 diluting agent Substances 0.000 claims abstract description 6
- 238000000034 method Methods 0.000 claims abstract description 6
- 239000012530 fluid Substances 0.000 claims description 75
- 238000010790 dilution Methods 0.000 claims description 71
- 239000012895 dilution Substances 0.000 claims description 71
- 239000012507 Sephadex™ Substances 0.000 claims description 52
- 239000002245 particle Substances 0.000 claims description 27
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 20
- 229940098773 bovine serum albumin Drugs 0.000 claims description 20
- 239000012141 concentrate Substances 0.000 claims description 20
- QLBHNVFOQLIYTH-UHFFFAOYSA-L dipotassium;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [K+].[K+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O QLBHNVFOQLIYTH-UHFFFAOYSA-L 0.000 claims description 20
- 239000007853 buffer solution Substances 0.000 claims description 16
- 239000008363 phosphate buffer Substances 0.000 claims description 12
- 239000002504 physiological saline solution Substances 0.000 claims description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- 239000003599 detergent Substances 0.000 claims description 7
- 238000002360 preparation method Methods 0.000 claims description 6
- 239000000725 suspension Substances 0.000 claims description 6
- 238000013016 damping Methods 0.000 claims description 5
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 4
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 4
- 229910000397 disodium phosphate Inorganic materials 0.000 claims description 4
- 235000019800 disodium phosphate Nutrition 0.000 claims description 4
- 235000013905 glycine and its sodium salt Nutrition 0.000 claims description 4
- 229910000403 monosodium phosphate Inorganic materials 0.000 claims description 4
- 235000019799 monosodium phosphate Nutrition 0.000 claims description 4
- 238000012856 packing Methods 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims description 4
- 239000002904 solvent Substances 0.000 claims description 4
- 239000000499 gel Substances 0.000 abstract description 91
- 230000035945 sensitivity Effects 0.000 abstract description 4
- 238000007873 sieving Methods 0.000 abstract 1
- 239000012224 working solution Substances 0.000 abstract 1
- 239000000427 antigen Substances 0.000 description 11
- 102000036639 antigens Human genes 0.000 description 11
- 108091007433 antigens Proteins 0.000 description 11
- 238000005516 engineering process Methods 0.000 description 6
- 239000000203 mixture Substances 0.000 description 4
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
- 206010067122 Haemolytic transfusion reaction Diseases 0.000 description 3
- 208000003441 Transfusion reaction Diseases 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 230000002949 hemolytic effect Effects 0.000 description 3
- 229910052700 potassium Inorganic materials 0.000 description 3
- 239000011591 potassium Substances 0.000 description 3
- 229920001503 Glucan Polymers 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 238000003018 immunoassay Methods 0.000 description 2
- TWNIBLMWSKIRAT-VFUOTHLCSA-N levoglucosan Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@H]2CO[C@@H]1O2 TWNIBLMWSKIRAT-VFUOTHLCSA-N 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- 101710175243 Major antigen Proteins 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 230000005875 antibody response Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 210000003617 erythrocyte membrane Anatomy 0.000 description 1
- 210000003754 fetus Anatomy 0.000 description 1
- 239000007863 gel particle Substances 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 210000002826 placenta Anatomy 0.000 description 1
- -1 polypropylene Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
Abstract
Description
Claims (5)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410801586.1A CN104569443B (zh) | 2014-12-22 | 2014-12-22 | 一种Rh血型检测卡 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410801586.1A CN104569443B (zh) | 2014-12-22 | 2014-12-22 | 一种Rh血型检测卡 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN104569443A true CN104569443A (zh) | 2015-04-29 |
CN104569443B CN104569443B (zh) | 2016-05-11 |
Family
ID=53085994
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201410801586.1A Active CN104569443B (zh) | 2014-12-22 | 2014-12-22 | 一种Rh血型检测卡 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN104569443B (zh) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105606829A (zh) * | 2015-12-30 | 2016-05-25 | 合肥天一生物技术研究所 | 一种新生儿血型鉴定卡 |
CN109669040A (zh) * | 2018-12-14 | 2019-04-23 | 厦门艾德生物医药科技股份有限公司 | 一种用于增强pd-l1单克隆抗体使用效果的抗体稀释液及其使用方法 |
CN111337693A (zh) * | 2020-03-30 | 2020-06-26 | 江苏中济万泰生物医药有限公司 | Rh血型检测卡的制备方法 |
CN112684176A (zh) * | 2020-12-04 | 2021-04-20 | 上海润普生物技术有限公司 | 一种Rh血型抗原检测卡及制备方法 |
CN113252914A (zh) * | 2021-06-23 | 2021-08-13 | 天津德祥生物技术有限公司 | 一种用于Rh系统分型检测卡的抗体稀释液及检测卡 |
Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040087032A1 (en) * | 1999-08-17 | 2004-05-06 | Chandler Mark B. | Microparticles with multiple fluorescent signals and methods of using same |
CN101718793A (zh) * | 2009-11-25 | 2010-06-02 | 江阴力博医药生物技术有限公司 | Rh血型分型卡的制备方法 |
CN102331504A (zh) * | 2011-10-21 | 2012-01-25 | 江苏中盛医学诊断试剂有限公司 | Abo-cde血型鉴定试剂卡及其制备方法 |
CN102435756A (zh) * | 2011-09-21 | 2012-05-02 | 长春博迅生物技术有限责任公司 | ABO、RhD血型定型检测卡的制备方法 |
CN102445550A (zh) * | 2010-10-09 | 2012-05-09 | 苏州苏大赛尔免疫生物技术有限公司 | ABO、RhD血型定型试剂卡及其制备方法及其采用的抗体稀释液 |
CN102507961A (zh) * | 2011-10-21 | 2012-06-20 | 江苏中盛医学诊断试剂有限公司 | 新生儿ABO/Rh血型鉴定试剂卡及其制备方法 |
WO2012112970A2 (en) * | 2011-02-18 | 2012-08-23 | Bio-Rad Laboratories, Inc. | Methods and compositions for detecting genetic material |
CN102680716A (zh) * | 2012-06-07 | 2012-09-19 | 北京金豪制药股份有限公司 | ABO/RhD血型抗原检测试剂卡及其制备 |
CN102707074A (zh) * | 2012-06-07 | 2012-10-03 | 北京金豪制药股份有限公司 | ABO/RhD血型定型检测试剂卡及其制备 |
-
2014
- 2014-12-22 CN CN201410801586.1A patent/CN104569443B/zh active Active
Patent Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040087032A1 (en) * | 1999-08-17 | 2004-05-06 | Chandler Mark B. | Microparticles with multiple fluorescent signals and methods of using same |
CN101718793A (zh) * | 2009-11-25 | 2010-06-02 | 江阴力博医药生物技术有限公司 | Rh血型分型卡的制备方法 |
CN102445550A (zh) * | 2010-10-09 | 2012-05-09 | 苏州苏大赛尔免疫生物技术有限公司 | ABO、RhD血型定型试剂卡及其制备方法及其采用的抗体稀释液 |
WO2012112970A2 (en) * | 2011-02-18 | 2012-08-23 | Bio-Rad Laboratories, Inc. | Methods and compositions for detecting genetic material |
CN102435756A (zh) * | 2011-09-21 | 2012-05-02 | 长春博迅生物技术有限责任公司 | ABO、RhD血型定型检测卡的制备方法 |
CN102331504A (zh) * | 2011-10-21 | 2012-01-25 | 江苏中盛医学诊断试剂有限公司 | Abo-cde血型鉴定试剂卡及其制备方法 |
CN102507961A (zh) * | 2011-10-21 | 2012-06-20 | 江苏中盛医学诊断试剂有限公司 | 新生儿ABO/Rh血型鉴定试剂卡及其制备方法 |
CN102680716A (zh) * | 2012-06-07 | 2012-09-19 | 北京金豪制药股份有限公司 | ABO/RhD血型抗原检测试剂卡及其制备 |
CN102707074A (zh) * | 2012-06-07 | 2012-10-03 | 北京金豪制药股份有限公司 | ABO/RhD血型定型检测试剂卡及其制备 |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105606829A (zh) * | 2015-12-30 | 2016-05-25 | 合肥天一生物技术研究所 | 一种新生儿血型鉴定卡 |
CN109669040A (zh) * | 2018-12-14 | 2019-04-23 | 厦门艾德生物医药科技股份有限公司 | 一种用于增强pd-l1单克隆抗体使用效果的抗体稀释液及其使用方法 |
CN111337693A (zh) * | 2020-03-30 | 2020-06-26 | 江苏中济万泰生物医药有限公司 | Rh血型检测卡的制备方法 |
CN112684176A (zh) * | 2020-12-04 | 2021-04-20 | 上海润普生物技术有限公司 | 一种Rh血型抗原检测卡及制备方法 |
CN113252914A (zh) * | 2021-06-23 | 2021-08-13 | 天津德祥生物技术有限公司 | 一种用于Rh系统分型检测卡的抗体稀释液及检测卡 |
Also Published As
Publication number | Publication date |
---|---|
CN104569443B (zh) | 2016-05-11 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN104569444B (zh) | 一种ABO正反定型及RhD血型检测卡 | |
CN104569443B (zh) | 一种Rh血型检测卡 | |
CN102445550B (zh) | 一种ABO、RhD血型定型试剂卡及其制备方法 | |
Christian | Studies of Aggregated γ-Globulin: I. Sedimentation, Electrophoretic and Anticomplementary Properties | |
CN101718794B (zh) | ABO/RhD血型定型检测试剂卡的制备方法 | |
CN103558400A (zh) | 一种降钙素原乳胶增强免疫比浊法检测试剂盒 | |
CN106290921A (zh) | 一种基于微孔膜的血型检测卡、血型检测系统 | |
CN101718793B (zh) | Rh血型分型卡的制备方法 | |
CN113252914B (zh) | 一种用于Rh系统分型检测卡的抗体稀释液及检测卡 | |
CN102707074A (zh) | ABO/RhD血型定型检测试剂卡及其制备 | |
CN102680716A (zh) | ABO/RhD血型抗原检测试剂卡及其制备 | |
CN102507961B (zh) | 新生儿ABO/Rh血型鉴定试剂卡及其制备方法 | |
CN101718784B (zh) | 新生儿溶血病检测试剂盒的制备方法 | |
CN105606829A (zh) | 一种新生儿血型鉴定卡 | |
CN105652014A (zh) | 一种直接抗人球蛋白检测卡的制备方法 | |
Moore et al. | A mouse monoclonal antibody with anti‐A,(B) specificity which agglutinates Ax cells | |
US3828103A (en) | Indirect hemagglutination test with simultaneous absorption of heterologous antibodies | |
CN205353104U (zh) | 一种ABO&RhD血型定型检测卡 | |
TWI242644B (en) | Method for screening autoantigen | |
CN101614736A (zh) | 应用新型全血样品垫处理方法的免疫层析检测试剂盒 | |
CN105092863A (zh) | 一种体外溶血试验配血方法 | |
CN104974218A (zh) | 低丰度差异蛋白的分离方法及其应用 | |
CN106940377A (zh) | 一种凝胶悬浮缓冲液、由其处理得到的凝胶、检测卡及其应用 | |
CN101718786B (zh) | 抗人球蛋白交叉配血卡的制备方法 | |
US3840655A (en) | Diagnostic methods and reagents for infectous mononucleosis |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Rh blood type detecting card Effective date of registration: 20171110 Granted publication date: 20160511 Pledgee: Industrial Bank Limited by Share Ltd. Hefei branch Pledgor: Hefei Tianyi Institute of Biological Technology Registration number: 2017340000338 |
|
CP03 | Change of name, title or address | ||
CP03 | Change of name, title or address |
Address after: 230000 the three floor of the 1 A workshop and the E area of Pearl River Industrial Park, No. 106, innovation Avenue, Hefei high tech Zone, Anhui. Patentee after: HEFEI TIANYI INSTITUTE OF BIOLOGICAL TECHNOLOGY Co.,Ltd. Address before: 230032 Anhui Haitang Road, Shushan District, Hefei Patentee before: Hefei Tianyi Institute of Biological Technology |
|
PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20190219 Granted publication date: 20160511 Pledgee: Industrial Bank Limited by Share Ltd. Hefei branch Pledgor: Hefei Tianyi Institute of Biological Technology Registration number: 2017340000338 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Rh blood type detecting card Effective date of registration: 20190530 Granted publication date: 20160511 Pledgee: Industrial Bank Limited by Share Ltd. Hefei branch Pledgor: HEFEI TIANYI INSTITUTE OF BIOLOGICAL TECHNOLOGY Co.,Ltd. Registration number: 2019340000275 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20191216 Granted publication date: 20160511 Pledgee: Industrial Bank Limited by Share Ltd. Hefei branch Pledgor: HEFEI TIANYI INSTITUTE OF BIOLOGICAL TECHNOLOGY Co.,Ltd. Registration number: 2019340000275 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Rh blood type detecting card Effective date of registration: 20191216 Granted publication date: 20160511 Pledgee: Jiujiang Bank Co.,Ltd. Hefei Dangtu Road sub branch Pledgor: HEFEI TIANYI INSTITUTE OF BIOLOGICAL TECHNOLOGY Co.,Ltd. Registration number: Y2019990000716 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20211126 Granted publication date: 20160511 Pledgee: Jiujiang Bank Co.,Ltd. Hefei Dangtu Road sub branch Pledgor: HEFEI TIANYI INSTITUTE OF BIOLOGICAL TECHNOLOGY Co.,Ltd. Registration number: Y2019990000716 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Rh blood group detection card Effective date of registration: 20211126 Granted publication date: 20160511 Pledgee: Jiujiang Bank Co.,Ltd. Hefei Dangtu Road sub branch Pledgor: HEFEI TIANYI INSTITUTE OF BIOLOGICAL TECHNOLOGY Co.,Ltd. Registration number: Y2021990001138 |
|
PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20221220 Granted publication date: 20160511 Pledgee: Jiujiang Bank Co.,Ltd. Hefei Dangtu Road sub branch Pledgor: HEFEI TIANYI INSTITUTE OF BIOLOGICAL TECHNOLOGY Co.,Ltd. Registration number: Y2021990001138 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Rh blood group detection card Effective date of registration: 20230113 Granted publication date: 20160511 Pledgee: Jiujiang Bank Co.,Ltd. Hefei Dangtu Road sub branch Pledgor: HEFEI TIANYI INSTITUTE OF BIOLOGICAL TECHNOLOGY Co.,Ltd. Registration number: Y2023980030914 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right |