CN104419653A - 一种分枝杆菌增菌液及其制备方法及应用 - Google Patents
一种分枝杆菌增菌液及其制备方法及应用 Download PDFInfo
- Publication number
- CN104419653A CN104419653A CN201310384401.7A CN201310384401A CN104419653A CN 104419653 A CN104419653 A CN 104419653A CN 201310384401 A CN201310384401 A CN 201310384401A CN 104419653 A CN104419653 A CN 104419653A
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- Prior art keywords
- mycobacterium
- enrichment liquid
- serum albumin
- bovine serum
- vitamin
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Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 20
- 239000012530 fluid Substances 0.000 title abstract 4
- 230000001954 sterilising effect Effects 0.000 claims abstract description 66
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 46
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims abstract description 42
- 238000002156 mixing Methods 0.000 claims abstract description 39
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims abstract description 37
- 229940098773 bovine serum albumin Drugs 0.000 claims abstract description 37
- 239000012153 distilled water Substances 0.000 claims abstract description 34
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 34
- 210000004556 brain Anatomy 0.000 claims abstract description 27
- 238000001802 infusion Methods 0.000 claims abstract description 27
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 claims abstract description 24
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 claims abstract description 24
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 claims abstract description 24
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000005642 Oleic acid Substances 0.000 claims abstract description 24
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 claims abstract description 24
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 claims abstract description 24
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims abstract description 23
- 235000011187 glycerol Nutrition 0.000 claims abstract description 23
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims abstract description 23
- 229920000053 polysorbate 80 Polymers 0.000 claims abstract description 23
- 239000011734 sodium Substances 0.000 claims abstract description 23
- 229910052708 sodium Inorganic materials 0.000 claims abstract description 23
- 239000002994 raw material Substances 0.000 claims abstract description 21
- 238000001914 filtration Methods 0.000 claims abstract description 15
- 241000186359 Mycobacterium Species 0.000 claims description 57
- 239000007788 liquid Substances 0.000 claims description 44
- 238000004659 sterilization and disinfection Methods 0.000 claims description 44
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 39
- 229930003756 Vitamin B7 Natural products 0.000 claims description 38
- 235000013372 meat Nutrition 0.000 claims description 38
- 239000000203 mixture Substances 0.000 claims description 38
- 235000014347 soups Nutrition 0.000 claims description 38
- 239000011735 vitamin B7 Substances 0.000 claims description 38
- 235000011912 vitamin B7 Nutrition 0.000 claims description 38
- 238000000034 method Methods 0.000 claims description 29
- 239000002253 acid Substances 0.000 claims description 22
- 108010046845 tryptones Proteins 0.000 claims description 17
- 239000011521 glass Substances 0.000 claims description 14
- 239000012528 membrane Substances 0.000 claims description 14
- 238000003756 stirring Methods 0.000 claims description 12
- 108010079058 casein hydrolysate Proteins 0.000 claims description 11
- 108010050327 trypticase-soy broth Proteins 0.000 claims description 2
- 238000001514 detection method Methods 0.000 abstract description 8
- 210000004369 blood Anatomy 0.000 abstract description 3
- 239000008280 blood Substances 0.000 abstract description 3
- 235000020958 biotin Nutrition 0.000 abstract 2
- 229960002685 biotin Drugs 0.000 abstract 2
- 239000011616 biotin Substances 0.000 abstract 2
- 238000005303 weighing Methods 0.000 abstract 2
- 235000010633 broth Nutrition 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 235000015424 sodium Nutrition 0.000 abstract 1
- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 abstract 1
- 241000187479 Mycobacterium tuberculosis Species 0.000 description 6
- 201000008827 tuberculosis Diseases 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 241000272761 Mycobacterium intracellulare ATCC 13950 Species 0.000 description 4
- 230000018044 dehydration Effects 0.000 description 4
- 238000006297 dehydration reaction Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 238000003745 diagnosis Methods 0.000 description 3
- 229960005486 vaccine Drugs 0.000 description 3
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 235000015278 beef Nutrition 0.000 description 2
- 244000309466 calf Species 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000007654 immersion Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000036457 multidrug resistance Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 241000186364 Mycobacterium intracellulare Species 0.000 description 1
- 108010064696 N,O-diacetylmuramidase Proteins 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 206010034133 Pathogen resistance Diseases 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 241000588769 Proteus <enterobacteria> Species 0.000 description 1
- 239000002647 aminoglycoside antibiotic agent Substances 0.000 description 1
- FRHBOQMZUOWXQL-UHFFFAOYSA-L ammonium ferric citrate Chemical compound [NH4+].[Fe+3].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O FRHBOQMZUOWXQL-UHFFFAOYSA-L 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 229940044197 ammonium sulfate Drugs 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000002785 anti-thrombosis Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 229960004676 antithrombotic agent Drugs 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 229910000365 copper sulfate Inorganic materials 0.000 description 1
- 229960000355 copper sulfate Drugs 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- PXEDJBXQKAGXNJ-QTNFYWBSSA-L disodium L-glutamate Chemical compound [Na+].[Na+].[O-]C(=O)[C@@H](N)CCC([O-])=O PXEDJBXQKAGXNJ-QTNFYWBSSA-L 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 235000019800 disodium phosphate Nutrition 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229960004642 ferric ammonium citrate Drugs 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000004313 iron ammonium citrate Substances 0.000 description 1
- 235000000011 iron ammonium citrate Nutrition 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 229960003390 magnesium sulfate Drugs 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000013923 monosodium glutamate Nutrition 0.000 description 1
- 230000000474 nursing effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 210000001539 phagocyte Anatomy 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 235000021317 phosphate Nutrition 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 description 1
- 239000011677 pyridoxine Substances 0.000 description 1
- 235000008160 pyridoxine Nutrition 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 230000002787 reinforcement Effects 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- 229940073490 sodium glutamate Drugs 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 229940038773 trisodium citrate Drugs 0.000 description 1
- 235000019263 trisodium citrate Nutrition 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- 229960001763 zinc sulfate Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (10)
Priority Applications (1)
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CN201310384401.7A CN104419653B (zh) | 2013-08-29 | 2013-08-29 | 一种分枝杆菌增菌液及其制备方法及应用 |
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CN201310384401.7A CN104419653B (zh) | 2013-08-29 | 2013-08-29 | 一种分枝杆菌增菌液及其制备方法及应用 |
Publications (2)
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CN104419653A true CN104419653A (zh) | 2015-03-18 |
CN104419653B CN104419653B (zh) | 2017-06-06 |
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CN201310384401.7A Active CN104419653B (zh) | 2013-08-29 | 2013-08-29 | 一种分枝杆菌增菌液及其制备方法及应用 |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112831539A (zh) * | 2021-01-27 | 2021-05-25 | 浙江夸克生物科技有限公司 | 一种需氧微生物的体外检测试剂盒 |
Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1430109A2 (en) * | 2001-09-27 | 2004-06-23 | Chiron Behring GmbH & Co. | Cultivation of mycobacteria |
CN1699592A (zh) * | 2005-04-29 | 2005-11-23 | 胡忠义 | 双相罗氏培养基及其制备方法 |
CN1858238A (zh) * | 2006-03-22 | 2006-11-08 | 李洪敏 | 快速结核分支杆菌培养基 |
CN101168766A (zh) * | 2007-11-13 | 2008-04-30 | 上海市肺科医院 | 双相罗氏鉴别或药敏培养基 |
CN101382549A (zh) * | 2008-09-24 | 2009-03-11 | 杭州创新生物检控技术有限公司 | 一种结核分枝杆菌快速检测系统及方法 |
CN102191196A (zh) * | 2011-03-24 | 2011-09-21 | 湖南省天骑医学新技术有限公司 | 结核分枝杆菌固、液培养基及其使用方法 |
CN102414324A (zh) * | 2009-05-04 | 2012-04-11 | 诺华有限公司 | 快速无菌微测试 |
CN102449137A (zh) * | 2008-12-05 | 2012-05-09 | 地中海(埃克斯-马赛第二)大学 | 包括结核分枝杆菌复合群分枝杆菌的分枝杆菌培养基和培养方法 |
-
2013
- 2013-08-29 CN CN201310384401.7A patent/CN104419653B/zh active Active
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1430109A2 (en) * | 2001-09-27 | 2004-06-23 | Chiron Behring GmbH & Co. | Cultivation of mycobacteria |
CN1699592A (zh) * | 2005-04-29 | 2005-11-23 | 胡忠义 | 双相罗氏培养基及其制备方法 |
CN1858238A (zh) * | 2006-03-22 | 2006-11-08 | 李洪敏 | 快速结核分支杆菌培养基 |
CN101168766A (zh) * | 2007-11-13 | 2008-04-30 | 上海市肺科医院 | 双相罗氏鉴别或药敏培养基 |
CN101382549A (zh) * | 2008-09-24 | 2009-03-11 | 杭州创新生物检控技术有限公司 | 一种结核分枝杆菌快速检测系统及方法 |
CN102449137A (zh) * | 2008-12-05 | 2012-05-09 | 地中海(埃克斯-马赛第二)大学 | 包括结核分枝杆菌复合群分枝杆菌的分枝杆菌培养基和培养方法 |
CN102414324A (zh) * | 2009-05-04 | 2012-04-11 | 诺华有限公司 | 快速无菌微测试 |
CN102191196A (zh) * | 2011-03-24 | 2011-09-21 | 湖南省天骑医学新技术有限公司 | 结核分枝杆菌固、液培养基及其使用方法 |
Non-Patent Citations (2)
Title |
---|
周俊梅: "几种结核分支杆菌培养方法的应用分析", 《中国实用医药》 * |
周贵民: "努力提高血液细菌培养阳性率", 《中华医学检验杂志》 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112831539A (zh) * | 2021-01-27 | 2021-05-25 | 浙江夸克生物科技有限公司 | 一种需氧微生物的体外检测试剂盒 |
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CN104419653B (zh) | 2017-06-06 |
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