CN104413120A - Black garlic and millet biscuit and preparation method thereof - Google Patents
Black garlic and millet biscuit and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
- A21D2/00—Treatment of flour or dough by adding materials thereto before or during baking
- A21D2/08—Treatment of flour or dough by adding materials thereto before or during baking by adding organic substances
- A21D2/36—Vegetable material
Abstract
The invention provides a black garlic and millet biscuit and a preparation method thereof. The black garlic and millet biscuit is prepared from the following raw materials in parts by weight: 10 to 15 parts of black garlic, 10 to 20 parts of powdered millet, 20 to 40 parts of wheat flour, 10 to 30 parts of inulin, 5 to 10 parts of egg white, and 30 to 35 parts of butter. The black garlic and millet biscuit is able to improve immunity of people and has the healthcare functions of controlling intestinal flora, preventing tumor, controlling blood glucose, blood lipid and blood pressure, and losing weight.
Description
Technical field
The present invention relates to field of food, specifically, the present invention relates to black garlic millet biscuit and preparation method thereof.
Background technology
Black garlic is the novel garlic products of one that fresh uncooked garlic processes through processes such as cleaning, enzyme, slaking, dryings.In product sensory, black garlic garlic clove is dark brown, soft and high resilience, soft rotten fragrant and sweet and without eating the pungent sense of fresh garlic and unhappy smell raw after entrance.In non-oxidizability and physiologically active, the fresh garlic of black garlic SOD specific activity exceeds more than 10 times, and the content of polyphenols also exceeds more than 5 times.
China is the major country of production of garlic, and its output accounts for 3/4ths of Gross World Product, Japan and southeast asian market on 80% garlic produced by China.Garlic is as a kind of important plant-based medicine and functional food ingredient, and its extract and preparation reach more than 1,500,000,000 dollars in the sales volume in the whole world, have huge potentiality to be exploited in China.In recent years, because people are to the generally accreditation of the numerous pharmacological action of black garlic and function, application and the demand of black garlic functional product were also increasing.But the exploitation at present for black garlic also just rests in the improvement of production technology mostly, and also little about the application of black garlic different physiological roles on food, thus, black garlic is applied in functional food, there is extremely important realistic meaning.
Summary of the invention
The present invention is intended to one of solve the problems of the technologies described above at least to a certain extent.For this reason, one object of the present invention is to propose a kind of black garlic millet biscuit and preparation method thereof, and this black garlic millet biscuit has regulating intestinal canal flora, regulates blood sugar, adjusting blood lipid, adjustment blood pressure, purposes in antitumor or treatment obesity.
Containing tetrahydro-β-carboline analog derivative in black garlic, therefore there is significant antioxidation activity.In addition, also the various active such as polypeptide, glycoprotein composition and trace elements of selenium, germanium etc. is contained in black garlic.As a kind of novel garlic products, the nutritional labeling contained by it and effect thereof are that the product such as Common garlic powder and garlic oil is irreplaceable.In addition, containing a large amount of functional components in black garlic, as polyphenol, sulfur-containing compound, β-carboline Alkaloid and melanoid etc.Their acting in conjunction, imparts the efficacy effect that black garlic is more powerful than fresh garlic.But the exploitation at present for black garlic also just rests in the improvement of production technology mostly, and yet there are no research and report about the application of black garlic multiple efficacies on food.
For this reason, in one aspect of the invention, the present invention proposes a kind of black garlic millet biscuit, this black garlic millet biscuit is made up of following raw material: the black garlic of 10 ~ 15 weight portions; The millet powder of 10 ~ 20 weight portions; The wheat flour of 20 ~ 40 weight portions; The inulin of 10 ~ 30 weight portions; The Egg-white of 5 ~ 10 weight portions; And 30 ~ 35 butter of weight portion.
Millet in above-mentioned raw materials is always famous to be of high nutritive value, and along with the raising of people's living standard, the requirement of people to food quality is also more and more higher, and has risen the upsurge of global edible five cereals.External going back is sold millet as nutritional health food.To character and the application of millet, existing research in ancient times, record according to medical book, millet nature and flavor are sweet, salty cool, the beneficial spleen of energy and stomach, old millet bitter cold, enter in taste, kidney and removing toxic substances, control stomach energy and quench one's thirst, diuresis.Cook congee and edible be not only easy to digestion, good palatability, and have benefiting pubic region, open the effect of stomach, external application also can be controlled Chi Dan and scald fire, burns.Millet per kilogram is containing 97 grams, protein, 35 grams, fat, and 728 grams, carbohydrate, except fat content is lower than except corn, all the other are every all high than other grain content.Also contain the necessary methionine of carrotene, Cobastab and human body, lysine, tryptophan etc. in millet, be the grain that a kind of nutritive value is higher simultaneously.
The nutritional labeling that the present inventor has for black garlic and health-care efficacy, to lie fallow edible health food black garlic millet biscuit for people with the matched preparation of millet is a kind of, this biscuit edible both can provide the abundant nutriment such as amino acid, vitamin, protein for human body, also be provided with regulating intestinal canal flora, give protection against cancer, prevent artery sclerosis, reduce blood pressure, the beneficial functional such as stabilizing blood sugar, anti-ageing, angiocardiopathy, be a kind of natural health care preferably.
The black garlic millet biscuit that the present invention proposes has gathered the dual-use function of black garlic and millet, makes this black garlic millet biscuit not only as simple food consumption, can also eat as functional food or health food simultaneously.According to a particular embodiment of the invention, inventor studies discovery, and black garlic millet biscuit tool in regulating intestinal canal flora has certain effect, and treatment obesity, raising immunity etc. is also had to effect of its uniqueness simultaneously.According to a particular embodiment of the invention, inventor studies further to this black garlic millet biscuit, find that long-term this black garlic millet biscuit edible also has regulating action to aspects such as blood sugar, blood fat, blood pressures, even at anti-tumor aspect, also there is certain co-treatment effect.Therefore black garlic is applied in functional food and there is extremely important realistic meaning.
In a second aspect of the present invention, the present invention proposes a kind of method preparing above-mentioned black garlic millet biscuit.According to a particular embodiment of the invention, it is even that the method can comprise the following steps: the butter of predetermined ratio, inulin, Egg-white, millet powder, wheat flour and black garlic to be carried out hybrid modulation, to obtain dough; Carry out dough mounting flower, shaping, to obtain biscuit embryo; And biscuit embryo is toasted; To obtain black garlic millet biscuit, wherein, predetermined ratio is: the black garlic of 10 ~ 15 weight portions; The millet powder of 10 ~ 20 weight portions; The wheat flour of 20 ~ 40 weight portions; The inulin of 10 ~ 30 weight portions; The Egg-white of 5 ~ 10 weight portions; And 30 ~ 35 butter of weight portion.
According to one embodiment of present invention, mouthfeel can effectively be prepared by said method good, the functional biscuit of successful.According to specifically embodiment of the present invention, the inulin in above-mentioned raw materials is also called synanthrin, is a kind of biological polyoses.Inulin is a kind of natural soluble dietary fiber, the viscosity of small intestine contents can be made in human body to increase, and the thickness of the non-stirring layer of intestinal mucosa is increased, thus delay and hinder the absorption of glucose and cholesterol, play the effect of reducing blood lipid, regulating blood glucose levels, and the fructose that its metabolism produces does not need insulin, therefore for diabetic, be a kind of desirable blood sugar reducing food.In addition, the selective proliferative substrate of inulin or Bifidobacterium, adult eats 5-20g inulin every day, and in 30 days rear intestinals, the quantity of Bifidobacterium can increase about 20 times.And the growth of Bifidobacterium has many positive influences to health: suppress harmful bacteria to increase, prevention and alleviate function of intestinal canal disorderly, reduce serum cholesterol, antitumor, develop immunitypty, delay senility, promote calcium, the absorption etc. of the metal ions such as magnesium, with black garlic with the use of, can synergic adjustment gut flora.
Therefore, the inulin used in the present invention not only can use as sweetener, increases the local flavor of black garlic millet biscuit, can also improve black garlic millet biscuit further and regulate the effect in blood sugar, blood fat etc.
According to one embodiment of present invention, the concrete operation step being carried out toasting by biscuit embryo in the method for above-mentioned preparation black garlic millet biscuit can comprise: by biscuit embryo roasting 15 minutes of fire under temperature is 180 degrees Celsius; And close fire maintenance 10 minutes.
According to one embodiment of present invention, above-mentionedly can to provide with the form of black garlic powder, black garlic juice or black garlic sauce for the preparation of the black garlic in black garlic millet biscuit raw material.According to a particular embodiment of the invention, black garlic powder can adopt following methods to prepare: select fresh black garlic of uniform size, peeling, clean the garlic clove of peeling, and clothing film is gone to the greatest extent, garlic clove to be placed in low-temperature drying equipment, constant temperature 5-6 hour under 60 DEG C of conditions, to water content lower than 5%; By dried garlic clove screening and removing impurities, pulverize until become powder with pulverizer, namely obtain black garlic powder with 80-100 mesh sieve.According to a particular embodiment of the invention, black garlic juice can adopt following methods to prepare: select fresh black garlic of uniform size, peeling, cleans the garlic clove of peeling, and clothing film is gone to the greatest extent, with stainless steel crusher in crushing 5min.To squeeze the juice 10min with juice extractor (8000rpm), then to filter after 5min through centrifuge with 60 order screen cloths and namely obtain black garlic juice.According to a particular embodiment of the invention ， ?garlic sauce following methods can be adopted to prepare: select fresh black garlic of uniform size, peeling, clean peeling garlic clove, and clothing film go use up, with stainless steel crusher in crushing 5min.Be added to concentration be 0.2 ~ 0.3 the vitamin C aqueous solution be 10 ~ 16% soak after 10 ~ 30 minutes and namely obtain black garlic sauce with black mashed garlic ratio.
According to one embodiment of present invention, the granularity of the black garlic powder of employing is also not particularly limited, and according to a particular embodiment of the invention, average grain diameter can be used to be that the black garlic powder of 60 ~ 150 microns is as raw material.The degree that the mouthfeel and being convenient to that can improve black garlic millet biscuit thus is further absorbed by the body and then play and improve body immunity, the effects such as regulating intestinal canal flora.
Conveniently understand, below the concrete operation step of the black garlic millet biscuit of preparation be described in detail:
Raw material: black garlic powder (juice or sauce) 10 ~ 15 weight portions, millet powder 10 ~ 20 weight portion, wheat flour 20 ~ 40 weight portion, inulin 10 ~ 30 weight portion, egg 5 ~ 10 weight portion, butter 30 ~ 35 weight portion.
Concrete operation step:
1, first butter is at room temperature melted for liquid;
2, divide and add inulin 2-3 time, stir 5min;
3, squeeze into Egg-white, stir 5min;
4, divide and be sieved into millet powder and wheat flour with flour bolt 2-3 time, stir 5min;
5, add black garlic powder (juice or sauce), stir 10 ~ 15min;
6, the dough piping bag be stirred is carried out mounting flower, shaping;
7, shaping biscuit embryo is put into baking oven, after about the 180 DEG C roasting 15min of fire, close the stewing 10min of fire;
8, black garlic millet biscuit is delivered to end from one end of cooling carrier bar, rejects undesirable goods;
9, select aluminium foil polyethylene complex pocket packaging, two pieces every bag, avoid the product moisture absorption and Oxidation of Fat and Oils.
Can prepare mouthfeel by above-mentioned steps good, nutritious, the black garlic millet biscuit that effect is complete, this biscuit is applicable to all kinds of crowd and eats, and is particularly useful for the elderly and eats for a long time.
Black garlic millet biscuit of the present invention has following advantage and beneficial effect:
1, all raw materials of black garlic millet biscuit are taken at nature, come from natural, safe and reliable, and raw material sources are extensively easy to get, and prescription compatibility is scientific and reasonable, determined curative effect, and preparation technology is simple, storing and application safety, low production cost.
2, not only can improve body immunity, but also there is regulating intestinal canal flora, antitumor, the health-care efficacy that regulates blood pressure and blood lipoid blood pressure, fat-reducing.
3, contained black garlic powder or black garlic juice, wherein black garlic powder is all ground into 100-230 object fine powder, and the active ingredient be conducive in raw material is dispersed, is convenient to the gastrointestinal absorption at human body.
4, black garlic millet biscuit of the present invention is obvious as functional food effect, security good, steady quality, and product can be preserved for a long time, for people's daily health caring provides new selection.
Additional aspect of the present invention and advantage will part provide in the following description, and part will become obvious from the following description, or be recognized by practice of the present invention.
Detailed description of the invention
Embodiments of the invention are described below in detail.Embodiment described below is exemplary, is intended to for explaining the present invention, and can not be interpreted as limitation of the present invention.
Embodiment 1
The parts by weight of raw material are: the black garlic juice of 10 weight portions, the millet powder of 20 weight portions, the wheat flour of 20 weight portions, the inulin of 10 weight portions, the egg of 10 weight portions, the butter of 30 weight portions.
The preparation of black garlic juice
Raw material: fresh black garlic
Preparation method: select fresh black garlic of uniform size, peeling, cleans the garlic clove of peeling, and clothing film is gone to the greatest extent, with stainless steel crusher in crushing 5min.To squeeze the juice 10min with juice extractor (8000rpm), then to filter after 5min through centrifuge with 60 order screen cloths and namely obtain black garlic juice.
First at room temperature melted by butter as liquid, point add inulin 2-3 time, stir 5min.Squeeze into Egg-white, stir 5min.Divide and be sieved into millet powder and wheat flour with flour bolt 2 ~ 3 times, stir 5min.Add black garlic juice, stir 10 ~ 15min.
The dough piping bag be stirred is carried out mounting flower, shaping.
Shaping biscuit embryo is put into baking oven, after about the 180 DEG C roasting 15min of fire, closes the stewing 10min of fire.
Finally, surface temperature is cooled to by biscuit to be normal temperature.
Embodiment 2
The parts by weight of raw material are: the black garlic powder of 10 weight portions, the millet powder of 25 weight portions, the wheat flour of 15 weight portions, the inulin of 12 weight portions, the egg of 8 weight portions, the butter of 30 weight portions.
The preparation of black garlic powder
Raw material: fresh black garlic
Preparation method: select fresh black garlic of uniform size, peeling, cleans the garlic clove of peeling, and clothing film is gone to the greatest extent, and garlic clove is placed in low-temperature drying equipment, and constant temperature 5-6 hour under 60 DEG C of conditions, to water content lower than 5%; By dried garlic clove screening and removing impurities, pulverize until become powder with pulverizer, namely obtain black garlic powder with 80-100 mesh sieve.
First at room temperature melted by butter as liquid, point add inulin 2-3 time, stir 5min.Squeeze into Egg-white, stir 5min.Divide and be sieved into millet powder and wheat flour with flour bolt 2-3 time, stir 5min.Add black garlic powder, stir 10 ~ 15min.
The dough piping bag be stirred is carried out mounting flower, shaping.
Shaping biscuit embryo is put into baking oven, after about the 180 DEG C roasting 15min of fire, closes the stewing 10min of fire.
Finally, surface temperature is cooled to by biscuit to be normal temperature.
Embodiment 3
The parts by weight of raw material are: the black garlic sauce of 5 weight portions, the millet powder of 30 weight portions, the wheat flour of 15 weight portions, the inulin of 12 weight portions, the egg of 8 weight portions, the butter of 30 weight portions.
The preparation of black garlic sauce
Raw material: fresh black garlic, the vitamin C aqueous solution
Preparation method: select fresh black garlic of uniform size, peeling, cleans the garlic clove of peeling, and clothing film is gone to the greatest extent, with stainless steel crusher in crushing 5min.Be added to concentration be 0.2 ~ 0.3 the vitamin C aqueous solution be 10 ~ 16% soak after 10 ~ 30 minutes and namely obtain black garlic sauce with black mashed garlic ratio.
First at room temperature melted by butter as liquid, point add inulin 2-3 time, stir 5min.Squeeze into Egg-white, stir 5min.Divide and be sieved into millet powder and wheat flour with flour bolt 2-3 time, stir 5min.
The dough piping bag be stirred is carried out mounting flower, shaping.
Shaping biscuit embryo is put into baking oven, after about the 180 DEG C roasting 15min of fire, closes the stewing 10min of fire.
Be cooled to by biscuit surface temperature to be normal temperature, black garlic sauce is smeared in the above, another biscuit sheet is placed on above black garlic sauce, is fixed, biscuit can not be out of shape.
Embodiment 4: functional food of the present invention is applied orally to rat and measures acute toxicity
6 week age specific-pathogen free (SPF) SD rat is utilized to carry out acute toxicity test.
The health food prepared by embodiment of the present invention 1-3 is suspended in the methocel solution of 0.5%, respectively with the dosage of 1g/kg, 5g/kg and 10g/kg once by oral administration to 5 the SD rats often organized, observe the death of rat, clinical symptoms and changes of weight, and carry out blood test and hematochemical test, when postmortem, any anomaly of visual inspection thorax abdomen gastrointestinal tissue.
Result shows: health food of the present invention does not cause any specific clinical symptoms, changes of weight or death, and blood test, hematochemical test and postmortem also do not change.Therefore, due to until the dosage of 10g/kg can not cause any poisoning change of rat, can judge that its median lethal dose (LD50) is far longer than 10g/kg, the health food that the present invention relates to is rated as safety non-toxic material.
Embodiment 5: functional food of the present invention regulates the research of human body intestinal canal flora impact
1. the grouping of test-meal experiment
The regulating intestinal canal flora function human experiment experimental evaluation way of embodiment 1-3 products obtained therefrom according to the Ministry of Public Health " health food inspection and assessment technical specification (2003) " is evaluated.
Here is the grouping of test-meal experiment:
Table 1 test-meal experiment grouping sheet
2. human experiment experimental technique
Experimenter is divided at random 6 groups, often organize 10 people, group result is as follows: group 1 is normal population, and group 2 is type ii diabetes patient, and group 3 is hyperlipemic patients, and group 4 is hyperpietic, and group 5 is tumor patient, and group 6 is obese patient.
Before experimenter's test-meal, all asepticly take experimenter's ight soil, 16S rDNA checks order inspection gut flora, as the reference of background level.
By the grouping situation of table 1, group 1 takes the made biscuit of embodiment 1, group 2 takes the made biscuit of embodiment 2, group 3 takes the made biscuit of embodiment 3, group 4 takes contrast biscuit, group 5 takes the made biscuit of embodiment 1, group 6 takes the made biscuit of embodiment 1, after 4 weeks, take experimenter's ight soil, 16SrDNA order-checking inspection gut flora, contrasts with background level, contrasts the multiple that its relative abundance increases.
3. gut flora detection method
First fecal sample carries out DNA extraction, V3 ~ V5 region of pcr amplification 16SrDNA, then with 454 platform order-checkings, and order-checking direction V5->V3.Average 7795 tag of each sample initial data, read long about 400bp.The final tag number average out to 3235 for analyzing, reads long about 265bp.
16S analyzes main mothur software (http://www.mothur.org/wiki/Mothur_manual) that adopts and comprises Quality Control, OTU cluster, the analyses such as annotation, and on the basis completing data species taxonomy, analyzes the relative abundance of each species.
4. experimental result
The sequencing data abundance contrast (improving multiple compared with the background level before test-meal) of each Pseudomonas of table 2 people gut flora
Experimental result shows, the gut flora of normal population, hyperglycaemia, high fat of blood, hyperpietic, tumor patient, obese patient all obtains improvement in various degree, functional product regulating intestinal canal flora Be very effective of the present invention.
Embodiment 6: functional food of the present invention is to the research of develop immunitypty function
One, test material
Animal used as test: BALB/C1 monthly age secondary mouse, male and female half and half, body weight 20 ± 2g, is in a good state of health, and is provided, production licence number by Wuhan University Experimental Animal Center: SCXK(Hubei Province) 2008-0004.Often criticize animal random packet.
Functional food described in the present embodiment is the black garlic millet biscuit prepared according to the formula of embodiment 1, RD is mouse 500mg/kgbw every day (body weight), recommends 0.5 times, 1 times and 2 times as low dose group (250mg/kgbw), middle dosage group (500mg/kgbw), high dose group (1000mg/kgbw) respectively using mouse.
If 4 positive controls,
Positive control 1 group is astragalus polyose capsule (primary raw material: the Radix Astragali, oligoisomaltose, Nei Monggol Shuangqi Pharmaceutical Co., Ltd., the strong word G20040082 of state's food),
Positive control 2 groups is FOS oral liquid (primary raw material: FOS, Zhuhai Shengyuan Biotechnology Co., Ltd., the strong word G20050336 of state's food),
Positive control 3 groups is chitosan oligosaccharide capsule (primary raw material: chitosan oligosaccharide, Xiamen Blue Bay Science and Technology Co., Ltd., the strong word G20110158 of state's food),
Positive control 4 groups is yeast dextran capsule (primary raw material: yeast dextran, Nanjing Potomac Beauty&Health Care Co., Ltd., the strong word G20110445 of state's food).
Other main reagent reagent is: sheep red blood cell (SRBC) (SRBC), Hank ' s liquid, GPS, prepared Chinese ink.
The wherein compound method of Hank ' s liquid:
(1) liquid is stored:
NaCl:80.00g, KCl:4.00g, Na
2hPO
412H
2o:1.52g, KH
2pO
4: 0.60g, glucose: 4.0g, 0.4% phenol red liquid: 50.00mL, two water that heats up in a steamer adds and is settled to 100mL, 115 DEG C of autoclaving 15min, stored frozen.
(2) working solution:
Add the two of 9 times of volumes to storage liquid and heat up in a steamer water, 115 DEG C of autoclaving 15min.Face the 5.6%NaHC03 solution adjust pH of used time sterilizing to 7.2-7.4 (solution is orange red).
The preparation of physiological saline: 9.00 grams of NaCl are dissolved in 991 grams pairs and heat up in a steamer in water the NaCl solution namely obtaining 0.9%.
Two, test method
Administration by gavage is taked in test.Every day gavage once, blank group fills with equal volume distilled water.Each group of mouse gives tested material after 30 days continuously, measures indices.
2.1 impacts on Mouse Weight, thymus gland/body weight, spleen/body weight
Mouse stomach de-neck execution afterwards in 30 days, weighs.Get Thymus and spleen, weigh Thymus and spleen weight respectively, measure thymus gland/body weight value, and spleen/body weight value.
2.2 impacts (mensuration of antibody-producting cell, hemolytic plaque test are the methods of the single antibody forming cell of a kind of vitro detection (thick liquid cell)) on mouse antibodies cellulation (PFC)
Lumbar injection 0.2mL2% (v/v) SRBC immunity every mouse.Immunity is after 5 days, and getting mouse spleen, to make cell concentration be 5 × 10
6the splenocyte liquid of individual/mL.Dissolve the solution that agarose makes l%, in 48 DEG C of water bath heat preservations, mix with Hank ' the s liquid of equivalent 2 times of concentration, divide and be filled in small test tube, often pipe 0.5mL.Add 0.05mL10%SRBC and 0.01mL splenocyte suspension, mix rapidly, be poured on slide.Incubation l h in 37 DEG C of insulating boxs, is added in complement (GPS) in slide groove, then incubation 1.5h.Counting plaque.
2.3 impacts (mensuration of serum hemolysin) on mouse hemolytic antibody nucleus formation
Lumbar injection 0.2mL2% (v/v) SRBC immunity every mouse.Immunity, after 5 days, is plucked eyeball and is got blood in centrifuge tube, places 1h, 3000rpm centrifugal 5 minutes, collects serum.Serum normal saline dilution 400 times, adds the mice serum 1.0mL of dilution, the SRBC0.5mL of 10% (v/v), complement (with physiological saline 1:10 dilution) 1.00mL successively.Separately establish the control tube of the not increase serum replaced with physiological saline.Put 37 DEG C of water-baths and after 20 minutes, set to 0 DEG C refrigerator 30 minutes stopped reactions, centrifugal 5 minutes of 3000rpm.Get supernatant 1.0mL, measure OD value in spectrophotometer 540nm wavelength place.
2.4 impacts on mouse carbonic clearance
Every mouse tail vein injection is diluted to the prepared Chinese ink of 4 times, 0.1mL/10g body weight.Timing immediately after prepared Chinese ink injects.2.0mL0.1%Na is added to respectively within l minute, 10 minutes, getting blood 0.02mL after injection prepared Chinese ink
2cO
3in solution (i.e. 1g/L), measure OD value in 600nm wavelength place, with Na
2cO
3solution compares.Separately get liver and spleen is weighed.The ability of mouse carbonic clearance is represented with phagocytic index (α).
Three, result of the test
The measurement result of 3.1 body weight, thymus gland/body weight, spleen/body weight
Each tested material is to Mouse Weight, thymus gland/body weight ratio, and the impact of spleen/body weight ratio, and measurement result is as shown in table 3,4 and 5.
The each tested material of table 3 is on the impact of Mouse Weight
As shown in Table 3, before per os gives the tested material of mouse various dose, and after 30 days, basic, normal, high 3 dosage groups are compared with blank group and positive controls, body weight, all without significant difference (P>0.05), namely feeds the sample of 3 basic, normal, high dosage groups and 4 positive controls to the body weight of mouse without impact to mouse.
Table 4 tested material is on the impact of mouse thymus/body weight ratio
As shown in Table 4, per os gave the tested material of mouse various dose after 30 days, compare with blank group, each dosage group thymus gland/all there were significant differences for body weight ratio (P < 0.05), namely increases all to some extent to the feed thymus gland/body weight ratio of sample to mouse of 3 dosage groups and 4 positive controls of mouse.
Table 5 tested material is on the impact of mouse spleen/body weight ratio
As shown in Table 5, per os gave the tested material of mouse various dose after 30 days, compare with blank group, each dosage group spleen/all there were significant differences for body weight ratio (P < 0.05), namely increases all to some extent to the feed spleen/body weight ratio of sample to mouse of 3 dosage groups and 4 positive controls of mouse.
3.2 antibody-producting cells (PFC) measurement result
Shown in antibody-producting cell (PFC) testing result table 6.
Table 6 tested material is on the impact of mouse antibodies cellulation number
As shown in Table 6, per os gave the tested material of mouse various dose after 30 days, compare with blank group, there were significant differences (P < 0.05) for the PFC quantity of positive control 1 group and low, middle dosage group, high dose group and 3 positive controls are described on mouse PFC without impact, and positive control 1 group, low dose group, middle dosage group there is remarkable impact to mouse PFC.
The measurement result of 3.3 serum hemolysins
The measurement result of serum hemolysin is as shown in table 7.
Table 7 tested material is on the impact of mice serum hemolysin
As shown in Table 7, per os gave the tested material of mouse various dose after 30 days, compared with blank group, the HC of low dose group, middle dosage group
50all there is pole significant difference (P < 0.01), the HC that positive control is 3 groups
50there were significant differences (P < 0.05), and low dose group of the present invention and middle dosage group are described, all can improve mice serum hemolysin HC significantly in pole
50, positive control 3 groups can significantly improve mice serum hemolysin HC
50, and positive control 1 group, positive control 2 groups, positive control 4 groups, high dose group are to mice serum hemolysin HC
50without impact.
3.4 mouse carbonic clearance experimental results
Mouse carbonic clearance experimental result is as shown in table 8.
Table 8 tested material is on the impact of mouse carbonic clearance
As shown in Table 8, per os gave the tested material of mouse various dose after 30 days, compare with blank group, middle dosage group, the high dose group of functional food of the present invention have pole significant difference (P<0.01) to mouse macrophage phagocytic activity, low dose group, positive control 1 group, positive control 2 groups have significant difference (P<0.05) to mouse macrophage phagocytic activity, remaining group on mouse carbonic clearance ability all without impact.This shows that functional food of the present invention has the effect strengthening mononuclear-macrophage phagocytic function, can strengthen the non-specific immune function of mouse.
Shown by result before and after the experiment of functional food raising immunity function of the present invention, each batch of Mice Body weight average all significantly increases without significant difference, thymus gland/body weight ratio, spleen/body weight ratio.In the experiment of mouse antibodies cellulation test experience, serum hemolysin determination experiment and mouse carbonic clearance, its result has significant difference.Can think that functional food of the present invention has the function and efficacy of develop immunitypty according to evaluation criterion, and the effect of functional food of the present invention to develop immunitypty is better than the action effect of the Radix Astragali of single component, FOS, chitosan oligosaccharide, yeast dextran.
Embodiment 7: the zoopery of functional food of the present invention in hypoglycemic
The auxiliary hyperglycemic function zoopery evaluation method of embodiment 1 products obtained therefrom according to the Ministry of Public Health " health food inspection and assessment technical specification (2003) " is evaluated.
1, test method: get male SD rat 30, prepares low dose of streptozotocin according to standard method and causes diabetes rat model, be divided into 4 groups at random after three days according to rat blood sugar value, often organizes 10.Each group of continuous gastric infusion 8 days, every day 1 time, last two hours after administration gets blood, measures blood glucose value.
2, subjects: tested SD rat is divided into 3 groups at random, often organize 10, tested SD rat primary diet control and movable constant, group 1 takes the made black garlic millet biscuit of embodiment 1, group 2 takes the made black garlic millet biscuit of black embodiment 2, group 3 takes the made black garlic millet biscuit of embodiment 3.
3, experimental result
The hypoglycemic result of different experiments group is as shown in table 9.
Table 9 different experiments group blood sugar decreasing effect compares
Note: (1) compares * P<0.01 with blank group; (2) compare with blank group
△p<0.05
Show from the animal test results of table 9, black garlic millet biscuit of the present invention all has decline effect to the hyperglycaemia of Experimental Diabetes of Mice, the black garlic millet biscuit that display embodiment 1-3 obtains all has obvious hypoglycemic function, the present invention has obvious technical advantage, has significant auxiliary therapeutic action.
Embodiment 8: the zoopery of functional food of the present invention in reducing blood lipid
The auxiliary lipid-lowering function zoopery evaluation method of embodiment 1-3 products obtained therefrom according to the Ministry of Public Health " health food inspection and assessment technical specification (2003) " is evaluated.
1, test material and method:
Wistar male rat 30, body weight 200 ± 20g, is provided by Wuhan University's Experimental Animal Center.Bring out rat hyperlipemia pathological model formula (high lipid food): add 1% cholesterol in basal feed, 10% yolk, 10% lard, is dried into block.Laboratory sample of the present invention black garlic millet biscuit functional food is provided by Shenzhen Huada Genetic Technology Co., Ltd, prepares by embodiments of the invention 1-3.
The preparation method of Hyperlipemia model rat: Wistar male rat is divided at random 3 groups (often organizing 10), i.e. hyperlipemia rat (hyperlipemia model control group), to feed high lipid food (high lipid food formula based on feed 93.8%, cholesterol 1.0%, lard 5.0%, cholate 0.2%), do not award tested material;
Medication:
Tested Hyperlipemia model rat is divided into 3 groups at random, often organize 10, the former diet control of tested Hyperlipemia model rat and movable constant, group 1 takes the made black garlic millet biscuit of embodiment 1, group 2 takes the made black garlic millet biscuit of black embodiment 2, group 3 takes the made black garlic millet biscuit of embodiment 3.
All animals are all raised 20 days under equivalent environment.After last gives the 4h of each tested material, get hematometry serum total cholesterol (TC mmol/L, iron chloride development process), serum triacylglycerol (TG mmol/L, acetylacetone method), HDL-C (HDL-C mmol/L, phosphotungstic acid method).
2, experimental result
The content of each group of rat blood serum total TG, TC and HDL-C is in table 10.
The effectiveness comparison of TC, TG, HDL-C falls in table 10 different experiments group
Note: (1) * represents P<0.05; (2) * * represents P<0.01
Result shows: the rat taking embodiment of the present invention 1-3 black garlic millet biscuit, compared with before test-meal, test-meal after 30 days Rat Cholesterol, triglyceride comparatively all have obvious reduction before test-meal, HDL comparatively has obvious rising before test-meal, and black garlic millet biscuit of the present invention has significant auxiliary therapeutic action to hyperlipidemia.
Embodiment 9: the human experiment experiment of functional food of the present invention in hypoglycemic, reducing blood lipid
Auxiliary hyperglycemic, the hypolipemic function human experiment experimental evaluation way of the foundation Ministry of Public Health " health food inspection and assessment technical specification (2003) " are evaluated.
1, present composition preparation:
Various different composition is prepared according to embodiment 1-3.
2, human experiment and test method:
Select 30 routine type II diabetes people, the wherein male sex 20 people by the principle of voluntariness, women 10 people, the range of age 43-75 year, without complication such as serious conscience kidneys, contrast design after taking in before test-meal.
Experimenter is divided into 3 groups at random, often organize 10 people, experimenter's former medicine kind, diet control and activity are constant, group 1 takes the made black garlic millet biscuit of embodiment 1, group 2 takes the made black garlic millet biscuit of black embodiment 2, group 3 takes the made black garlic millet biscuit of embodiment 3, daily 1 time, in one after each meal, take 30 days continuously.
The change of monitoring patient blood pressure, stool and urine, body weight, and the blood sugar (with glucose oxidase method) measuring empty stomach and 2 hours after the meal; Measure blood fat (T-CHOL TC, triglycerides TG and HDL HDL-C).
3, judgment criteria:
Effective: cardinal symptom disappears, on an empty stomach or the blood sugar of 2 hours after the meal comparatively treat before decline and be no more than 30.0%.
Effective: cardinal symptom obviously improves, on an empty stomach and the blood sugar of 2 hours after the meal comparatively treat before decline and be no more than 10.0%.
Invalid: cardinal symptom is not improved, on an empty stomach and the blood sugar of 2 hours after the meal comparatively treat before decline and be less than 10.0%.
4, human experiment result:
Test-meal, after 30 days, takes the patient of each group of composition, compared with before test-meal, and the blood pressure of patient, stool and urine, body weight there are no significant difference, but fasting blood-glucose, 2h-plasma glucose all have obvious attenuating, and concrete outcome is in table 11.
About the patient impact of the blood fat before and after test-meal being taken to each group of composition, compared with before test-meal, the cholesterol of test-meal patient after 30 days, triglyceride comparatively all have obvious reduction before test-meal, and HDL comparatively has obvious rising before test-meal, and concrete outcome is in table 12.
As can be seen here, embodiment 1-3 of the present invention black garlic millet biscuit has the health care of obvious auxiliary hyperglycemic, reducing blood lipid, and to the harmless effect of tested population health.
Table 11 foretastes crowd's blood glucose test results
Table 12 foretastes crowd's lipids detection result
Note: (1) * represents P<0.05; (2) * * represents P<0.01
Embodiment 10: functional food of the present invention is in the zoopery of anti-tumor aspect
The auxiliary lipid-lowering function zoopery evaluation method of embodiment 1-3 products obtained therefrom according to the Ministry of Public Health " health food inspection and assessment technical specification (2003) " is evaluated.
1, test material and method:
BALB/c mouse (4-6 age in week, male, body weight 20-24g) 40, is seeded in the right armpit of BALB/c mouse by the hepatoma cells MM45T.Li of exponential phase subcutaneous, every only inoculation 5 × 10
7individual cell, sets up Transplanted tumor model, and laboratory sample of the present invention black garlic millet biscuit is provided by BGI-Shenzhen, prepares by embodiments of the invention 1-3.
Medication:
Tested rat model is divided into 4 groups at random, often organize 10, former diet control and movable constant, group 1 takes the made black garlic millet biscuit of embodiment 1, group 2 takes the made black garlic millet biscuit of black embodiment 2, group 3 takes the made black garlic millet biscuit of embodiment 3, group 4 takes physiological saline, successive administration 30 days.Observe ordinary circumstance and tumor size change before and after mouse treatment.
Become after knurl and measure the most major diameter (a) of 1 tumour and most minor axis (b) with the every 2d of slide measure, obtain knurl body approximate volumes (TV: volume) by formula TV=a × b2/2.Evaluate medicine tumor-inhibiting action by the following method: put to death animal in the 2d of last administration and take tumor weight, and press following formula:
Calculate medicine tumour inhibiting rate (IR): tumor growth tumour inhibiting rate=(heavy-Hei garlic millet cake dry food group of the average knurl of control group average knurl weight) average knurl of/control group heavy × 100%.Evaluate Antitumor Activity of Drugs effect with inhibition rate of tumor growth IR, the standard of curative effect evaluation: IR < 30 is invalid, IR >=30 are effective.
The effectiveness comparison of table 13 different experiments group Tumor growth inhibition
Result shows: the rat taking black garlic millet biscuit described in embodiment 1-3, compared with control group, test-meal after 30 days medicine tumour inhibiting rate all have obvious reduction, black garlic millet biscuit antagonism tumour of the present invention has significant auxiliary therapeutic action.
Embodiment 11: functional food of the present invention is tested hypertensive result for the treatment of
30 routine hyperpietics are selected to carry out clinical observation to the curative effect of medicine of the present invention.All cases all according to the diagnostic criteria of " new Chinese medicine clinical guidance principle (first volume) ", is diagnosed as hypertension.
Diagnostic criteria
Systolic pressure is equal to or higher than 160mmHg(21.3kPa), diastolic pressure is equal to or higher than 95mmHg(12.721.3kPa), both have 1 through examining, and can make a definite diagnosis.
Methods for the treatment of
Design with own control, experimenter is divided into 3 groups at random, often organize 10 people, by the grouping situation of table 1, take the made black garlic millet biscuit of embodiment 1 by group 1, group 2 takes the made black garlic millet biscuit of black embodiment 2, group 3 takes the made black garlic millet biscuit of embodiment 3, daily 1 time, in one after each meal, take 30 days continuously.Be consistent before diet and amount of exercise and test during test-meal.Indices in test-meal on-test and at the end of each test 1 time.
According to the criterion of therapeutical effect in " new Chinese medicine clinical guidance principle (first volume) ", effective: 1. diastolic pressure decline 10mmHg(1.3kPa) more than, and reach normal range (NR); Though 2. diastolic pressure is normally not near, with decline 20mmHg(2.7kPa) or more, wherein 1 must be possessed; Effective: 1. diastolic pressure declines not as good as 10mmHg(1.3kPa), but to reach normal range (NR); 2. diastolic pressure comparatively treats the front 10 ~ 19mmHg(1.3 ~ 2.5kPa that declines), but do not reach normal range (NR); 3. systolic pressure comparatively treats front decline 30mmHg(4kPa); Wherein 1 must be possessed; Invalid: not reach above standard.
After two groups of case treatments, comparitive study the results are shown in Table 14.
Blood pressure (diastolic pressure) measurement result (mmHg) before and after table 14 test-meal
Note: (1) * represents effective; (2) * * represents effective
In this test-meal test, experimenter takes black garlic millet biscuit after 30 days continuously, and blood pressure all has decline in various degree.As can be seen here, black garlic millet biscuit of the present invention has obvious hypotensive activity to human body, and has no bad reaction during taking.
Embodiment 12: functional food of the present invention is tested the result for the treatment of of obesity
1, test material and method
Each experiment product of the present invention, is provided by BGI-Shenzhen, prepares according to embodiment of the present invention 1-3.
Study subject: through health check-up Pass Test requirement, the fat volunteer of overweight degree more than 20% 30, without Other diseases, viscera function is normal.
Method: adopt own control design, experimenter is divided into 3 groups at random, often organize 10 people, take the made black garlic millet biscuit of embodiment 1 by group 1, group 2 takes the made black garlic millet biscuit of black embodiment 2, group 3 takes the made black garlic millet biscuit of embodiment 3, daily 1 time, in one after each meal, take 30 days continuously.Be consistent before diet and amount of exercise and test during test-meal.Indices in test-meal on-test and at the end of each test 1 time.
2, both effectiveness observation
1. height (cm), body weight (kg) calculate SBW, overweight degree is measured:
Adult's SBW (kg)=[height (cm)-100] × 0.9
Overweight degree (%)=(actual measurement body weight-SBW)/SBW × 100%
2. body fat total amount (kg) and fat percentage (%) measure: measure Determination of Total Body Fat with electrical impedance instrument.
3. waistline, hip circumference (cm) are measured: use tape measuring.
3, observed result
3.1 body weight, body fat total amount and fat percentage measure
About to the body weight before and after test-meal, body fat, take the patient of each group of black garlic millet cake dry food, compared with before test-meal, the body weight of test-meal patient after 30 days, body fat comparatively all have obvious reduction before test-meal, Rate of body lipid comparatively has obvious rising before test-meal, and concrete outcome is in table 15.
Body weight, body fat measurement result before and after table 15 test-meal
Note: (1) * represents P<0.05; (2) * * represents P<0.01
The measurement of 3.2 waistlines, hip circumference
About to the waistline before and after test-meal, hip circumference, take the patient of each group of composition, compared with before test-meal, the waistline of test-meal patient after 30 days, hip circumference comparatively all have obvious reduction before test-meal, and concrete outcome is in table 16.
Table 16 test-meal front and back waist, hip circumference measurement result
Note: (1) * represents P<0.05; (2) * * represents P<0.01
In this test-meal test, experimenter takes black garlic millet biscuit after 30 days continuously, and body weight, body fat total amount, Rate of body lipid, waistline, hip circumference all significantly decline.As can be seen here, black garlic millet biscuit of the present invention has obvious antiobesity action to human body, and has no bad reaction during taking.
In the description of this description, specific features, structure, material or feature that the description of reference term " embodiment ", " some embodiments ", " example ", " concrete example " or " some examples " etc. means to describe in conjunction with this embodiment or example are contained at least one embodiment of the present invention or example.In this manual, identical embodiment or example are not necessarily referred to the schematic representation of above-mentioned term.And the specific features of description, structure, material or feature can combine in an appropriate manner in any one or more embodiment or example.
Although illustrate and describe embodiments of the invention above, be understandable that, above-described embodiment is exemplary, can not be interpreted as limitation of the present invention, those of ordinary skill in the art can change above-described embodiment within the scope of the invention when not departing from principle of the present invention and aim, revising, replacing and modification.
Claims (7)
1. a black garlic millet biscuit, is characterized in that, is be made up of following raw material:
The black garlic of 10 ~ 15 weight portions;
The millet powder of 10 ~ 20 weight portions;
The wheat flour of 20 ~ 40 weight portions;
The inulin of 10 ~ 30 weight portions;
The Egg-white of 5 ~ 10 weight portions; And
The butter of 30 ~ 35 weight portions.
2. black garlic millet biscuit according to claim 1, is characterized in that, described black garlic millet biscuit is functional food or health food.
3. the black garlic millet biscuit described in claim 1 or 2 regulating intestinal canal flora, regulate blood sugar, adjusting blood lipid, adjustment blood pressure, antitumor or treatment obesity in purposes.
4. prepare a method for black garlic millet biscuit, it is characterized in that,
The butter of predetermined ratio, inulin, Egg-white, millet powder, wheat flour and black garlic are carried out hybrid modulation even, to obtain dough;
Carry out described dough mounting flower, shaping, to obtain biscuit embryo; And
Described biscuit embryo is toasted; To obtain described black garlic millet biscuit,
Wherein, described predetermined ratio is:
The black garlic of 10 ~ 15 weight portions;
The millet powder of 10 ~ 20 weight portions;
The wheat flour of 20 ~ 40 weight portions;
The inulin of 10 ~ 30 weight portions;
The Egg-white of 5 ~ 10 weight portions; And
The butter of 30 ~ 35 weight portions.
5. method according to claim 4, is characterized in that, described baking comprises:
Described biscuit embryo fire under temperature is 180 degrees Celsius is baked 15 minutes; And
Close fire to keep 10 minutes.
6. method according to claim 4, is characterized in that, described black garlic provides with the form of black garlic powder, black garlic juice or black garlic sauce.
7. method according to claim 6, is characterized in that, the average grain diameter of described black garlic powder is 60 ~ 150 microns.
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| CN107259557A (en) * | 2017-07-21 | 2017-10-20 | 杜亚东 | A kind of nutrient powder of weight-reducing |
| CN110024835A (en) * | 2019-05-13 | 2019-07-19 | 黑龙江八一农垦大学 | A kind of production method of pinenut shortbread type biscuit |
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