CN104404057A - 一种大鲵虹彩病毒病疫苗及制备方法和应用 - Google Patents
一种大鲵虹彩病毒病疫苗及制备方法和应用 Download PDFInfo
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- CN104404057A CN104404057A CN201410728186.2A CN201410728186A CN104404057A CN 104404057 A CN104404057 A CN 104404057A CN 201410728186 A CN201410728186 A CN 201410728186A CN 104404057 A CN104404057 A CN 104404057A
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Abstract
Description
pPICZαB载体片段 | 3μL |
截短优化MCP基因片段 | 2μL |
10×T4DNA Ligase buffer | 1μL |
T4DNA Ligase | 1μL |
ddH2O | 补至10μL |
ddH2O | 29μL |
10×buffer J | 10μL |
质粒DNA(0.4μg/μL) | 50μL |
SacⅠ | 10μL |
BSA | 1μL |
实验组 | 接种物 | 剂量 | 数量 |
A组 | 大鲵虹彩病毒病酵母疫苗(本发明研制) | 5微克/100微升 | 90尾 |
B组 | 大鲵虹彩病毒病酵母疫苗(本发明研制) | 10微克/100微升 | 90尾 |
C组 | 大鲵虹彩病毒病酵母疫苗(本发明研制) | 15微克/100微升 | 90尾 |
D组 | 大鲵虹彩病毒病酵母疫苗(本发明研制) | 20微克/100微升 | 90尾 |
E组 | 阳性对照(大鲵虹彩病毒细胞灭活疫苗) | 100微升 | 90尾 |
F组 | 生理盐水对照 | 100微升 | 90尾 |
实验组 | 接种物 | 存活数 |
A组 | 大鲵虹彩病毒病酵母疫苗(本发明研制) | 68尾 |
B组 | 大鲵虹彩病毒病酵母疫苗(本发明研制) | 71尾 |
C组 | 大鲵虹彩病毒病酵母疫苗(本发明研制) | 73尾 |
D组 | 大鲵虹彩病毒病酵母疫苗(本发明研制) | 85尾 |
E组 | 阳性对照(大鲵虹彩病毒细胞灭活疫苗) | 82尾 |
F组 | 生理盐水对照 | 18尾 |
Claims (6)
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CN104774262A (zh) * | 2015-04-21 | 2015-07-15 | 中国检验检疫科学研究院 | 抗大鲵虹彩病毒单克隆抗体及其制备与应用 |
CN110279854A (zh) * | 2019-07-19 | 2019-09-27 | 暨南大学 | 一种大口黑鲈病毒dna疫苗及其制备方法与应用 |
CN111394318A (zh) * | 2020-03-16 | 2020-07-10 | 中国水产科学研究院长江水产研究所 | 一种大鲵虹彩病毒弱毒株及其应用 |
CN116024247A (zh) * | 2022-12-07 | 2023-04-28 | 广东省农业科学院动物卫生研究所 | 一种鳜蛙虹彩病毒疫苗及其制备方法和应用 |
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CN102643835A (zh) * | 2012-04-28 | 2012-08-22 | 中国水产科学研究院长江水产研究所 | 大鲵虹彩病毒主衣壳蛋白的原核表达及多抗制备和应用 |
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Publication number | Priority date | Publication date | Assignee | Title |
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CN104774262A (zh) * | 2015-04-21 | 2015-07-15 | 中国检验检疫科学研究院 | 抗大鲵虹彩病毒单克隆抗体及其制备与应用 |
CN104774262B (zh) * | 2015-04-21 | 2017-10-20 | 中国检验检疫科学研究院 | 抗大鲵虹彩病毒单克隆抗体及其制备与应用 |
CN110279854A (zh) * | 2019-07-19 | 2019-09-27 | 暨南大学 | 一种大口黑鲈病毒dna疫苗及其制备方法与应用 |
CN111394318A (zh) * | 2020-03-16 | 2020-07-10 | 中国水产科学研究院长江水产研究所 | 一种大鲵虹彩病毒弱毒株及其应用 |
CN116024247A (zh) * | 2022-12-07 | 2023-04-28 | 广东省农业科学院动物卫生研究所 | 一种鳜蛙虹彩病毒疫苗及其制备方法和应用 |
CN116024247B (zh) * | 2022-12-07 | 2023-08-04 | 广东省农业科学院动物卫生研究所 | 一种鳜蛙虹彩病毒疫苗及其制备方法和应用 |
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