CN104357401A - 可分泌抗ii型登革病毒ns1单克隆抗体的杂交瘤细胞、单克隆抗体及应用 - Google Patents
可分泌抗ii型登革病毒ns1单克隆抗体的杂交瘤细胞、单克隆抗体及应用 Download PDFInfo
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- CN104357401A CN104357401A CN201410606685.4A CN201410606685A CN104357401A CN 104357401 A CN104357401 A CN 104357401A CN 201410606685 A CN201410606685 A CN 201410606685A CN 104357401 A CN104357401 A CN 104357401A
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106290847A (zh) * | 2015-05-13 | 2017-01-04 | 上海凯创生物技术有限公司 | 一种登革热病毒ns1抗原乳胶法检测试剂盒 |
CN106309278A (zh) * | 2016-08-31 | 2017-01-11 | 澳宝化妆品(惠州)有限公司 | 用于抗衰老化妆品的龙舌兰发酵物及其制备方法和应用 |
CN106701812A (zh) * | 2016-12-27 | 2017-05-24 | 中国医学科学院医学生物学研究所 | 一种i‑iv型串联登革病毒edⅲ重组蛋白的构建方法及其应用 |
CN107177557A (zh) * | 2017-05-04 | 2017-09-19 | 菲鹏生物股份有限公司 | 可分泌抗h‑fabp单克隆抗体的杂交瘤细胞、单克隆抗体及其制备方法和应用 |
CN110007095A (zh) * | 2019-05-06 | 2019-07-12 | 江苏硕世生物科技股份有限公司 | 一种登革病毒ns1抗原检测试纸条、试剂盒及其制备方法 |
WO2020043066A1 (zh) * | 2018-08-28 | 2020-03-05 | 东莞市朋志生物科技有限公司 | Ns1蛋白的结合蛋白 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102161983A (zh) * | 2011-03-28 | 2011-08-24 | 中山大学 | Ⅰ-iv型登革病毒ns1单克隆抗体杂交瘤细胞株及其试剂盒 |
US20130164734A1 (en) * | 2011-12-22 | 2013-06-27 | Inbios International, Inc. | Methods and materials for the detection of dengue virus infection |
CN103396481A (zh) * | 2013-07-18 | 2013-11-20 | 华南理工大学 | 一种二型登革热病毒ns1蛋白的重链单域抗体及其制备方法和应用 |
-
2014
- 2014-10-30 CN CN201410606685.4A patent/CN104357401B/zh active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102161983A (zh) * | 2011-03-28 | 2011-08-24 | 中山大学 | Ⅰ-iv型登革病毒ns1单克隆抗体杂交瘤细胞株及其试剂盒 |
US20130164734A1 (en) * | 2011-12-22 | 2013-06-27 | Inbios International, Inc. | Methods and materials for the detection of dengue virus infection |
CN103396481A (zh) * | 2013-07-18 | 2013-11-20 | 华南理工大学 | 一种二型登革热病毒ns1蛋白的重链单域抗体及其制备方法和应用 |
Non-Patent Citations (5)
Title |
---|
E.A.HENCHAL,ET AL: "Synergistic Interactins of Anti-NS1 Monoclonal Antibodies Protect Passively Immunized Mice from Lethal Challenge with Dengue 2 Virus", 《JOURNAL OF GENERAL VIROLOGY》 * |
LI-WEN QIU,ET AL: "Development of an Antigen Capture Immunoassay Based on Monoclonal Antibodies Specific for Dengue Virus Serotype 2 Nonstructural Protein 1 for Early and Rapid Identification of Dengue Virus", 《CLINICAL AND VACCINE IMMUNOLOGY》 * |
严炎炎等: "2型登革病毒NS1蛋白单克隆抗体的生物学特性研究", 《实用预防医学》 * |
但妍等: "抗2型登革病毒M蛋白和NS1蛋白单克隆抗体的制备及鉴定", 《中国优秀硕士论文全文数据库》 * |
李多等: "重组Ⅱ型登革病毒NS1的表达及其免疫原性的研究", 《中国生物工程杂志》 * |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106290847A (zh) * | 2015-05-13 | 2017-01-04 | 上海凯创生物技术有限公司 | 一种登革热病毒ns1抗原乳胶法检测试剂盒 |
CN106309278A (zh) * | 2016-08-31 | 2017-01-11 | 澳宝化妆品(惠州)有限公司 | 用于抗衰老化妆品的龙舌兰发酵物及其制备方法和应用 |
CN106701812A (zh) * | 2016-12-27 | 2017-05-24 | 中国医学科学院医学生物学研究所 | 一种i‑iv型串联登革病毒edⅲ重组蛋白的构建方法及其应用 |
CN107177557A (zh) * | 2017-05-04 | 2017-09-19 | 菲鹏生物股份有限公司 | 可分泌抗h‑fabp单克隆抗体的杂交瘤细胞、单克隆抗体及其制备方法和应用 |
WO2020043066A1 (zh) * | 2018-08-28 | 2020-03-05 | 东莞市朋志生物科技有限公司 | Ns1蛋白的结合蛋白 |
CN110007095A (zh) * | 2019-05-06 | 2019-07-12 | 江苏硕世生物科技股份有限公司 | 一种登革病毒ns1抗原检测试纸条、试剂盒及其制备方法 |
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