CN104311691A - 一种苦瓜多糖的提取方法 - Google Patents
一种苦瓜多糖的提取方法 Download PDFInfo
- Publication number
- CN104311691A CN104311691A CN201410628576.2A CN201410628576A CN104311691A CN 104311691 A CN104311691 A CN 104311691A CN 201410628576 A CN201410628576 A CN 201410628576A CN 104311691 A CN104311691 A CN 104311691A
- Authority
- CN
- China
- Prior art keywords
- balsam pear
- enzymolysis
- add
- bitter
- bitter melon
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 244000302512 Momordica charantia Species 0.000 title claims abstract description 90
- 235000009811 Momordica charantia Nutrition 0.000 title claims abstract description 90
- 150000004676 glycans Chemical class 0.000 title claims abstract description 82
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 82
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 82
- 238000000605 extraction Methods 0.000 title abstract description 13
- 238000000034 method Methods 0.000 claims abstract description 38
- 102000004190 Enzymes Human genes 0.000 claims abstract description 26
- 108090000790 Enzymes Proteins 0.000 claims abstract description 26
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 25
- 108090000145 Bacillolysin Proteins 0.000 claims abstract description 17
- 102000035092 Neutral proteases Human genes 0.000 claims abstract description 17
- 108091005507 Neutral proteases Proteins 0.000 claims abstract description 17
- 238000001556 precipitation Methods 0.000 claims abstract description 17
- 239000000843 powder Substances 0.000 claims abstract description 16
- 235000009812 Momordica cochinchinensis Nutrition 0.000 claims description 49
- 235000018365 Momordica dioica Nutrition 0.000 claims description 49
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 238000001914 filtration Methods 0.000 claims description 14
- 238000010298 pulverizing process Methods 0.000 claims description 14
- 239000006228 supernatant Substances 0.000 claims description 14
- 239000000284 extract Substances 0.000 claims description 13
- 230000007062 hydrolysis Effects 0.000 claims description 10
- 238000006460 hydrolysis reaction Methods 0.000 claims description 10
- 238000000874 microwave-assisted extraction Methods 0.000 claims description 9
- 239000012141 concentrate Substances 0.000 claims description 7
- 230000006837 decompression Effects 0.000 claims description 7
- 238000001035 drying Methods 0.000 claims description 7
- 239000000706 filtrate Substances 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 7
- 238000002156 mixing Methods 0.000 claims description 7
- 239000002994 raw material Substances 0.000 claims description 7
- 239000002893 slag Substances 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 7
- 229940088598 enzyme Drugs 0.000 abstract description 23
- 235000019441 ethanol Nutrition 0.000 abstract 2
- 108090000526 Papain Proteins 0.000 abstract 1
- 239000004365 Protease Substances 0.000 abstract 1
- 229920002678 cellulose Polymers 0.000 abstract 1
- 235000010980 cellulose Nutrition 0.000 abstract 1
- 238000004140 cleaning Methods 0.000 abstract 1
- 238000009833 condensation Methods 0.000 abstract 1
- 230000005494 condensation Effects 0.000 abstract 1
- 238000004108 freeze drying Methods 0.000 abstract 1
- 229940055729 papain Drugs 0.000 abstract 1
- 235000019834 papain Nutrition 0.000 abstract 1
- 238000007796 conventional method Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000001117 sulphuric acid Substances 0.000 description 4
- 238000000975 co-precipitation Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000002137 ultrasound extraction Methods 0.000 description 2
- 238000003809 water extraction Methods 0.000 description 2
- 208000020446 Cardiac disease Diseases 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 235000009815 Momordica Nutrition 0.000 description 1
- 241000218984 Momordica Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 235000002597 Solanum melongena Nutrition 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- WQZGKKKJIJFFOK-PQMKYFCFSA-N alpha-D-mannose Chemical compound OC[C@H]1O[C@H](O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-PQMKYFCFSA-N 0.000 description 1
- SRBFZHDQGSBBOR-QMKXCQHVSA-N alpha-L-arabinopyranose Chemical compound O[C@H]1CO[C@@H](O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-QMKXCQHVSA-N 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 229940088623 biologically active substance Drugs 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 239000012043 crude product Substances 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000004438 eyesight Effects 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 208000019622 heart disease Diseases 0.000 description 1
- 201000001421 hyperglycemia Diseases 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 230000003914 insulin secretion Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000452 restraining effect Effects 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 229940124549 vasodilator Drugs 0.000 description 1
- 239000003071 vasodilator agent Substances 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
Description
Claims (5)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410628576.2A CN104311691B (zh) | 2014-11-11 | 2014-11-11 | 一种苦瓜多糖的提取方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410628576.2A CN104311691B (zh) | 2014-11-11 | 2014-11-11 | 一种苦瓜多糖的提取方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN104311691A true CN104311691A (zh) | 2015-01-28 |
CN104311691B CN104311691B (zh) | 2016-12-14 |
Family
ID=52367020
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201410628576.2A Active CN104311691B (zh) | 2014-11-11 | 2014-11-11 | 一种苦瓜多糖的提取方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN104311691B (zh) |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105820262A (zh) * | 2016-03-31 | 2016-08-03 | 唐云龙 | 一种苦瓜多糖的提取方法 |
CN106832027A (zh) * | 2016-12-30 | 2017-06-13 | 浙江海洋大学 | 秋葵多糖的提取方法 |
CN107674904A (zh) * | 2017-11-02 | 2018-02-09 | 林峰 | 苦瓜活性肽、组合物及制备方法 |
CN108586560A (zh) * | 2018-03-23 | 2018-09-28 | 汕头大学 | 一种具有降糖活性的苦瓜皂苷和多糖双水相一步提取方法 |
CN109280091A (zh) * | 2018-11-27 | 2019-01-29 | 苏州卫生职业技术学院 | 一种苦瓜多糖的生物酶提取工艺 |
CN111227224A (zh) * | 2018-11-10 | 2020-06-05 | 江西乐迪医疗科技有限公司 | 一种有助于保护糖尿病肾病患者肾功能的医学配方食品 |
CN112704232A (zh) * | 2020-12-30 | 2021-04-27 | 杭州娃哈哈科技有限公司 | 一种可用于自由基清除的苦瓜籽提取物及制备方法和应用 |
CN113817077A (zh) * | 2021-10-08 | 2021-12-21 | 广西壮族自治区水产科学研究院 | 一种长茎葡萄蕨藻多糖的提取方法 |
CN115594775A (zh) * | 2022-11-07 | 2023-01-13 | 福建医颜科生物科技有限公司(Cn) | 一种银耳多糖的制备方法及应用 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20050245737A1 (en) * | 2004-04-22 | 2005-11-03 | Cummings Richard D | Apparatus and method for mixed-bed lectin chromatography |
CN101147537A (zh) * | 2007-10-26 | 2008-03-26 | 华南理工大学 | 一种从苦瓜中高效提取苦瓜多糖的方法 |
JP4261071B2 (ja) * | 1999-04-15 | 2009-04-30 | タカラバイオ株式会社 | 治療剤 |
CN102746412A (zh) * | 2012-06-26 | 2012-10-24 | 安徽亿源生物工程有限公司 | 一种苦瓜多糖的提取方法 |
CN102936295A (zh) * | 2012-12-11 | 2013-02-20 | 青岛海洋新材料科技有限公司 | 一种苦瓜多糖的提取方法 |
-
2014
- 2014-11-11 CN CN201410628576.2A patent/CN104311691B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP4261071B2 (ja) * | 1999-04-15 | 2009-04-30 | タカラバイオ株式会社 | 治療剤 |
US20050245737A1 (en) * | 2004-04-22 | 2005-11-03 | Cummings Richard D | Apparatus and method for mixed-bed lectin chromatography |
CN101147537A (zh) * | 2007-10-26 | 2008-03-26 | 华南理工大学 | 一种从苦瓜中高效提取苦瓜多糖的方法 |
CN102746412A (zh) * | 2012-06-26 | 2012-10-24 | 安徽亿源生物工程有限公司 | 一种苦瓜多糖的提取方法 |
CN102936295A (zh) * | 2012-12-11 | 2013-02-20 | 青岛海洋新材料科技有限公司 | 一种苦瓜多糖的提取方法 |
Non-Patent Citations (2)
Title |
---|
周东坡等: "《生物制品学(第1版)》", 31 May 2007, 化学工业出版社 * |
张恒弼等: "《中药现代研究与开发(第1版)》", 31 January 2010, 中国科学技术出版社 * |
Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105820262A (zh) * | 2016-03-31 | 2016-08-03 | 唐云龙 | 一种苦瓜多糖的提取方法 |
CN106832027A (zh) * | 2016-12-30 | 2017-06-13 | 浙江海洋大学 | 秋葵多糖的提取方法 |
CN107674904A (zh) * | 2017-11-02 | 2018-02-09 | 林峰 | 苦瓜活性肽、组合物及制备方法 |
CN108586560A (zh) * | 2018-03-23 | 2018-09-28 | 汕头大学 | 一种具有降糖活性的苦瓜皂苷和多糖双水相一步提取方法 |
CN111227224A (zh) * | 2018-11-10 | 2020-06-05 | 江西乐迪医疗科技有限公司 | 一种有助于保护糖尿病肾病患者肾功能的医学配方食品 |
CN109280091A (zh) * | 2018-11-27 | 2019-01-29 | 苏州卫生职业技术学院 | 一种苦瓜多糖的生物酶提取工艺 |
CN112704232A (zh) * | 2020-12-30 | 2021-04-27 | 杭州娃哈哈科技有限公司 | 一种可用于自由基清除的苦瓜籽提取物及制备方法和应用 |
CN113817077A (zh) * | 2021-10-08 | 2021-12-21 | 广西壮族自治区水产科学研究院 | 一种长茎葡萄蕨藻多糖的提取方法 |
CN115594775A (zh) * | 2022-11-07 | 2023-01-13 | 福建医颜科生物科技有限公司(Cn) | 一种银耳多糖的制备方法及应用 |
CN115594775B (zh) * | 2022-11-07 | 2023-08-08 | 福建医颜科生物科技有限公司 | 一种银耳多糖的制备方法及应用 |
Also Published As
Publication number | Publication date |
---|---|
CN104311691B (zh) | 2016-12-14 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN104311691B (zh) | 一种苦瓜多糖的提取方法 | |
CN101486651B (zh) | 一种杜仲叶中提取绿原酸的方法及其应用 | |
CN102321189B (zh) | 一种木耳多糖的综合提取工艺 | |
CN103555556B (zh) | 以玉米芯和玉米穗为原料发酵制备人参醋的方法 | |
CN108003251B (zh) | 一种菠萝皮渣多糖的提取方法 | |
CN101838302B (zh) | 一种油茶皂苷的提取方法 | |
CN110684128B (zh) | 一种黄精多糖的提取精制方法 | |
CN102199225B (zh) | 一种复合酶法制备茶树菇水解液的方法 | |
CN106036341A (zh) | 一种提高诺丽果粉中有效成分的方法 | |
CN102382205A (zh) | 一种从香蕉皮中提取果胶的方法 | |
CN104311689A (zh) | 一种黄芪多糖的提取方法 | |
CN101955548B (zh) | 酶法辅助提取蒺藜多糖的方法 | |
CN104231107A (zh) | 一种海带粗多糖的提取方法 | |
CN101974096B (zh) | 一种提取裙带菜孢子叶多糖的方法 | |
CN108277248A (zh) | 一种超声波提取沙棘活性多肽的方法 | |
CN106282266A (zh) | 一种利用柚苷酶制备浒苔寡糖的方法 | |
CN102134285A (zh) | 一种残次坛紫菜多糖的制备方法 | |
CN106832021A (zh) | 一种肉桂渣粗多糖的制备方法 | |
CN104292355B (zh) | 一种桔梗多糖的提取方法 | |
CN102191295A (zh) | 一种从杏鲍菇废菌渣中提取多糖的方法 | |
CN108998491A (zh) | 一种提取牡丹籽粕中蛋白质的方法 | |
CN105012778A (zh) | 一种铁皮石斛薏苡仁胶囊及其制备方法 | |
CN102146143A (zh) | 人参、西洋参提取渣制备果胶及纤维素的方法和应用 | |
CN108753872B (zh) | 一种莲子多糖高收率的提取方法 | |
CN103382230B (zh) | 从西瓜皮提取果胶的方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C41 | Transfer of patent application or patent right or utility model | ||
CB03 | Change of inventor or designer information |
Inventor after: Tan Mingcong Inventor before: Chi Zonglei Inventor before: Zang Weigong Inventor before: Ding Zhenyang |
|
COR | Change of bibliographic data | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20161012 Address after: 401320 Chongqing city Banan District Yudong textile Three Village No. 1 Applicant after: CHONGQING LUDI MACHINERY FACTORY Address before: Tianhe District Tong East Road Guangzhou city Guangdong province 510665 B-101 No. 5, room B-118 Applicant before: GUANGDONG GAOHANG INTELLECTUAL PROPERTY OPERATION Co.,Ltd. Effective date of registration: 20161012 Address after: Tianhe District Tong East Road Guangzhou city Guangdong province 510665 B-101 No. 5, room B-118 Applicant after: GUANGDONG GAOHANG INTELLECTUAL PROPERTY OPERATION Co.,Ltd. Address before: Watson Road, Licheng District 250100 in Shandong city of Ji'nan province No. 389 building 401. Applicant before: JINAN CAVIN BIOTECHNOLOGY Co.,Ltd. |
|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20190321 Address after: 518116 4-5 floors, Building C, No. 9 Dolly Industrial Plant, Jinlong Road, Baolong Street, Longgang District, Shenzhen City, Guangdong Province Patentee after: SHENZHEN VIGOR HEALTH-CARE PRODUCTS Co.,Ltd. Address before: No. 1, No. three village, fish cave textile, Chongqing, Banan District, Chongqing Patentee before: CHONGQING LUDI MACHINERY FACTORY |
|
TR01 | Transfer of patent right | ||
CP01 | Change in the name or title of a patent holder |
Address after: 518116 4-5 floors, Building C, No. 9 Dolly Industrial Plant, Jinlong Road, Baolong Street, Longgang District, Shenzhen City, Guangdong Province Patentee after: Huazhen Biotechnology (Shenzhen) Co.,Ltd. Address before: 518116 4-5 floors, Building C, No. 9 Dolly Industrial Plant, Jinlong Road, Baolong Street, Longgang District, Shenzhen City, Guangdong Province Patentee before: SHENZHEN VIGOR HEALTH-CARE PRODUCTS Co.,Ltd. |
|
CP01 | Change in the name or title of a patent holder |