CN104285636B - A kind of abiogenous method of manual-induced wheat scab - Google Patents

A kind of abiogenous method of manual-induced wheat scab Download PDF

Info

Publication number
CN104285636B
CN104285636B CN201410478253.XA CN201410478253A CN104285636B CN 104285636 B CN104285636 B CN 104285636B CN 201410478253 A CN201410478253 A CN 201410478253A CN 104285636 B CN104285636 B CN 104285636B
Authority
CN
China
Prior art keywords
wheat
ditch
furrow
irrigation
manual
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201410478253.XA
Other languages
Chinese (zh)
Other versions
CN104285636A (en
Inventor
邓云
谢冬容
许卿
周仕全
饶溶晖
邱慧明
黄继平
马妙明
陈柳凤
胡忠全
刘梦莹
肖翔
胡蕾
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
FUJIAN NANPING AGRICULTURAL SCIENCE INSTITUTE
Original Assignee
FUJIAN NANPING AGRICULTURAL SCIENCE INSTITUTE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by FUJIAN NANPING AGRICULTURAL SCIENCE INSTITUTE filed Critical FUJIAN NANPING AGRICULTURAL SCIENCE INSTITUTE
Priority to CN201410478253.XA priority Critical patent/CN104285636B/en
Publication of CN104285636A publication Critical patent/CN104285636A/en
Application granted granted Critical
Publication of CN104285636B publication Critical patent/CN104285636B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention discloses a kind of manual-induced abiogenous method of wheat scab, it includes site preparation and plays furrow, broadcasts sowing the step such as broken straw, flood irrigation, cover film in spite of illness.The present invention does not need artificial infection time-consuming like that using manual-induced wheat natural occurrence, unrestricted for examination wheat quantity, is conducive to the screening and identification of big mass breeding material, and overcoming artificial inoculation can only identification of species（Strain）Anti gibberellic disease extended capability, the shortcoming of the anti-infection ability for being unfavorable for identifying wheat, while the process that manual control natural occurrence also infects again closer to the circulation of the upper pathogen of production, qualification result is more accurate.

Description

A kind of abiogenous method of manual-induced wheat scab
Technical field
The present invention relates to wheat breeding for disease resistance field, more particularly to a kind of abiogenous side of manual-induced wheat scab Method.
Background technology
Wheat scab (Gibberella zeae (Schw.) Perch.) is distributed widely in China's warm moist or half Humid area, is mainly in Upper Yangtze River, middle and lower reaches and the Yellow River and Huai He River south section, and the harm to Wheat Production is very big.Sick wheat not only can Severe Reduction is caused, can more deteriorate grain quality.Deoxynivalenol (DON) strong toxicity that head blight is produced, eats After can cause the acute poisoning symptom such as dizziness, fever, nausea,vomiting,diarrhea, influence immunocompetence and reduce fecundity etc., Harm human and livestock health., once detection toxin DON, i.e., can not eat, DON appetite is more than 2mg/kg just for developed country's regulation in the world Feed can not be made.
The fine wheat varieties of seed selection anti gibberellic disease, are most effective and environmentally friendly to prevent and treat wheat scab method.Seed selection resists The wheat breed of head blight must can just be screened in the case where head blight occurs.In 1954-2013, head blight is big Popular 8 years, moderate is popular 15 years, is within remaining 27 years disease-free or hypopathia year, and popular frequency is 46%, with obvious intermittence.Cause How this controls every harvest wheat head blight fully, stably to fall ill in wheat scab disease resistance Breeding Process, it is ensured that in morbidity Under conditions of carry out pressure selection, be screen wheat-resistance to scab kind key issue.At present, the artificial side for inducing head blight Method is mainly and coordinates artificial moisturizing to complete by artificial inoculation.Artificial inoculation is by indoor culture pathogen and by people's work order Flower drop post is completed, and because workload is big, its cultivation that cannot realize big colony's wheat scab material cannot also simulate nature hair The circulation infection processs of disease.
The content of the invention
Induced so as to realize wheat scab by realizing big colony's wheat scab it is an object of the invention to provide one kind The method of big colony's identification.
Realizing the technical scheme of the object of the invention is：A kind of abiogenous method of manual-induced wheat scab, its bag Include following steps：
1）Site preparation plays furrow：The good field of selection irrigation condition does furrow, is offered around furrow ditch vertical direction and field and furrow The size in the irrigation and drainage ditch of ditch connection, furrow ditch and gutter has ponding to realize irrigation ditch, and furrow ditch is moistened but without ponding；
2）Broadcast sowing broken straw in spite of illness：The straw with gibberellic hypha that the wheat heading initial stage crushes is sown on the wheat head, is allowed Pathogen sprouts and infects naturally；
3）Flood irrigation：Ear the initial stage in wheat, cooperation is while broadcast sowing broken straw in spite of illness, flood irrigation field, from heading stage to whole Individual period of disease field humidity ensures that there is ponding in irrigation and drainage ditch, and furrow ditch is moistened but without ponding；
4）Cover film：5~7 days after wheat flowering to flowering, plastic film shed is built, it is ensured that humidity exists in greenhouse 80%~100%, after film is covered 5~7 days, remove film；The covering of film can not only improve temperature of shed, accelerate water in field ditch Divide and issue additional and prevent diffusion, reach the requirement for keeping humidity in canopy, while build hot and humid environmental benefits being given birth in pathogen Long and circulation is infected again, it is ensured that wheat identification of species is fully fallen ill.
For superior technique effect, technical solution of the present invention can also do following improvement：
1st, the step 1）Length≤the 20m of middle furrow, width is 1~1.2m, and depth is 0.2~0.3m.Furrow are oversize, unfavorable It is too short to be unfavorable for land utilization ratio in draining；1~1.2 meter of width can sow 30~50 seeds, convenient sowing and after Phase incidence survey, width is too wide to be unfavorable for investigation, and width is too narrow, and the plant lazy weight planted is for pilot survey.
2nd, the step 1）Middle furrow ditch depth and width are 20~30cm, are offered around furrow ditch vertical direction and field The irrigation and drainage ditch connected with furrow ditch, row irrigation ditch width and depth are 40~50cm, it is ensured that draining is convenient, keep furrow ditch Ensure the moistening of field air while moistening, the water in furrow ditch can be discharged during rainy season, other times can be used for storing Water, has ponding both to ensure field surface moisture in irrigation and drainage ditch, also do not result in wheat root and flooded.
3rd, the step 1）The depth in middle irrigation and drainage ditch is at least compared with furrow ditch depth 20cm, it is ensured that during rainy season can quickly by Water in furrow ditch is discharged.
4th, the step 4）Middle greenhouse length is 30~40m, width is 6~12m, is highly 2.5~3.5m.Greenhouse specification The excessive stability for being unfavorable for ensureing greenhouse structure, specification is too small to cause the waste of material and labour cost.
5th, bird prevention net is set after removing film, it is ensured that wheat identification of species is not by bird damage.
The beneficial effects of the invention are as follows not needing artificial infection to take a lot of work like that expense using manual-induced wheat natural occurrence When, it is unrestricted for examination wheat quantity, be conducive to the screening and identification of big mass breeding material, instant invention overcomes artificial inoculation Can only identification of species（Strain）Anti gibberellic disease extended capability, be unfavorable for identify wheat anti-infection ability shortcoming, while people It is the process that control natural occurrence also infects again closer to the circulation of the upper pathogen of production, qualification result is more accurate.
Specific embodiment
Technical solution of the present invention is described further with reference to embodiments.
Embodiment 1：A kind of abiogenous method of manual-induced wheat scab, it is comprised the following steps：
1）Site preparation plays furrow：The good field of selection irrigation condition does furrow, and the length of furrow is 20m, and width is 1m, and depth is 0.2m；Furrow ditch depth and width are 20cm, and the irrigation and drainage ditch connected with furrow ditch is offered around furrow ditch vertical direction and field, Row irrigation ditch width and depth are 50cm；The depth in irrigation and drainage ditch is at least compared with furrow ditch depth 20cm；
5）Broadcast sowing broken straw in spite of illness：The straw with gibberellic hypha that the wheat heading initial stage crushes is sown on the wheat head, is allowed Pathogen sprouts and infects naturally；
6）Flood irrigation：Ear the initial stage in wheat, cooperation is while broadcast sowing broken straw in spite of illness, flood irrigation field, from heading stage to whole Individual period of disease field humidity ensures that there is ponding in irrigation and drainage ditch, and furrow ditch is moistened but without ponding；
7）Cover film：5~7 days after wheat flowering to flowering, plastic film shed is built, it is ensured that humidity exists in greenhouse 80%~100%；Greenhouse length is 40m, width is 12m, is highly 3.5m；
5）Bird prevention net is set：After film is covered 5~7 days, film is removed, bird prevention net is set, it is ensured that wheat identification of species is not by bird Class is destroyed.
Embodiment 2：
Using method same as Example 1, difference is that the length of furrow is 18m, and width is 1.2m, and depth is 0.3m； Furrow ditch depth and width are 25cm, and row irrigation ditch width and depth are 45cm；Greenhouse length is 37m, width is 10m, high It is 3m to spend.
Embodiment 3：
Using method same as Example 1, difference is that the length of furrow is 16m, and width is 1.1m, and depth is 0.2m； Furrow ditch depth and width are 25cm, and row irrigation ditch width and depth are 43cm.Greenhouse length is 35m, width is 8m, height It is 2.8m.
Embodiment 4：
Using method same as Example 1, difference is that the length of furrow is 15m, and width is 1.2m, and depth is 0.3m； Furrow ditch depth and width are 30cm, and row irrigation ditch width and depth are 40cm；Greenhouse length is 30m, width is 6m, height It is 2.5m.
Identified, wheat scab induction rate reaches 100% in above-mentioned 4 embodiments.
Embodiments of the invention are the foregoing is only, the scope of the claims of the invention is not thereby limited, it is every to utilize this hair Equivalent structure or equivalent flow conversion that bright description is made, or directly or indirectly it is used in other related technology necks Domain, is included within the scope of the present invention.

Claims (6)

1. a kind of abiogenous method of manual-induced wheat scab, it is comprised the following steps：
1) site preparation plays furrow：The good field of selection irrigation condition does furrow, is offered around furrow ditch vertical direction and field and connected with furrow ditch Logical irrigation and drainage ditch, furrow ditch and irrigation and drainage ditch size have ponding to realize irrigation and drainage ditch, and furrow ditch is moistened but without ponding；
2) broken straw in spite of illness is broadcasted sowing：The straw with gibberellic hypha of wheat heading initial stage crushing is sown on the wheat head, allows cause of disease Bacterium sprouts and infects naturally；
3) flood irrigation：Ear the initial stage in wheat, while broken straw in spite of illness is broadcasted sowing in cooperation, flood irrigation field, from heading stage to whole hair Stadium field humidity ensures that there is ponding in irrigation and drainage ditch, and furrow ditch is moistened but without ponding；
4) cover film：5~7 days after wheat flowering to flowering, build plastic film shed, it is ensured that in greenhouse humidity 80%~ 100%, after film is covered 5~7 days, remove film.
2. the abiogenous method of manual-induced wheat scab according to claim 1, it is characterised in that：The step 1) length≤20m of furrow in, width is 1~1.2m, and depth is 0.2~0.3m.
3. the abiogenous method of manual-induced wheat scab according to claim 1, it is characterised in that：It is described：Institute State step 1) in furrow ditch depth and width be 20~30cm, row irrigation ditch width and depth are 40~50cm.
4. the abiogenous method of manual-induced wheat scab according to claim 1, it is characterised in that：The step 1) depth in irrigation and drainage ditch is at least compared with furrow ditch depth 20cm in.
5. the abiogenous method of manual-induced wheat scab according to claim 1, it is characterised in that：The step 4) in greenhouse length be 30~40m, width be 6~12m, be highly 2.5~3.5m.
6. the abiogenous method of manual-induced wheat scab according to claim 1, it is characterised in that：Set after removing film Put bird prevention net.
CN201410478253.XA 2014-09-19 2014-09-19 A kind of abiogenous method of manual-induced wheat scab Active CN104285636B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201410478253.XA CN104285636B (en) 2014-09-19 2014-09-19 A kind of abiogenous method of manual-induced wheat scab

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410478253.XA CN104285636B (en) 2014-09-19 2014-09-19 A kind of abiogenous method of manual-induced wheat scab

Publications (2)

Publication Number Publication Date
CN104285636A CN104285636A (en) 2015-01-21
CN104285636B true CN104285636B (en) 2017-07-04

Family

ID=52305809

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201410478253.XA Active CN104285636B (en) 2014-09-19 2014-09-19 A kind of abiogenous method of manual-induced wheat scab

Country Status (1)

Country Link
CN (1) CN104285636B (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104969789B (en) * 2015-07-03 2017-05-03 湖北省农业科学院粮食作物研究所 Field humidifying device for inducing wheat scab and use method thereof
CN112514745B (en) * 2020-11-24 2023-08-04 安徽省农业科学院烟草研究所 High-throughput screening method suitable for corn ear rot resistance germplasm

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR0009629A (en) * 2000-12-08 2002-08-20 Embrapa - Empresa Brasileira De Pesquisa Agropecua Biocontrol of plant diseases caused by fusarium species with new isolates of bacillus megaterium and pantoea agglomerans
CN100506016C (en) * 2007-04-02 2009-07-01 冯成玉 Application of wheat gibberellic disease by wet weather forecast
CN101690451B (en) * 2009-09-18 2011-04-20 安徽省农业科学院烟草研究所 Ralstonia solanacearum disease resistance high density identification method in field
CN102599014A (en) * 2012-03-14 2012-07-25 浙江省农业科学院 Method for artificially inducing field wheat scab

Also Published As

Publication number Publication date
CN104285636A (en) 2015-01-21

Similar Documents

Publication Publication Date Title
CN102726342B (en) Ecological guinea pig breeding technique
CN103270918B (en) Rice and shrimp joint culturing ecological breeding system and method
CN103314785B (en) A kind of method of ramie straw cultivation Stropharia rugoso-annulata
CN104365465B (en) The interplanting device and its cultural method of Herba Dendrobii and Herba Anoectochili roxburghii
CN102696555B (en) Semi-wild manual Cordyceps-cultivating method
CN103493686B (en) Method for cultivating tricholoma lobayense heim through mulberry twig stems and cassava stems
CN105918200B (en) Crab and rice symbiotic breeding pond and comprehensive temperature adjusting method thereof
CN103539540B (en) Method for culturing tricholoma lobayense by using mulberry branches
CN105028077A (en) Novel three-dimensional planting method adopted in rice field
CN103918537A (en) Processing method for Dendrobium officinale culture substrate
CN103477965A (en) Field cultivation seedbed for dendrobium officinale kimura et migo
CN105165405A (en) Northeast forest land stropharia rugoso-annulata cultivation method
CN104081990A (en) Method for stereoscopic planting of vegetables in greenhouse
CN111066575A (en) Guangxi red globe mushroom field ecological cultivation method
CN102792920B (en) Earthworm culturing system for large-scale biological treatment on sludge
CN109907004B (en) Grub field breeding method based on edible fungus matrix
CN105660190B (en) Complementary symbiotic three-dimensional cultivation and breeding method for lucid ganoderma and wood frogs
CN104285636B (en) A kind of abiogenous method of manual-induced wheat scab
CN106922545A (en) A kind of cultivating system of Dabie Mountain black goat
CN107027714A (en) A kind of edible earthworm indoor culture method
CN106258475A (en) A kind of stropharia rugoso-annulata plantation method being suitable to cold ground
CN105191668A (en) Poria cocos cultivation technology
Nimachow et al. A sustainable mountain paddy fish farming of the Apatani tribes of Arunachal Pradesh, India
CN108002901A (en) Stropharia rugoso-annulata plantation base-material and preparation method thereof
CN111296366B (en) Industrial earthworm cultivation method in open air environment

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
CB03 Change of inventor or designer information
CB03 Change of inventor or designer information

Inventor after: Deng Yun

Inventor after: Hu Zhongquan

Inventor after: Liu Mengying

Inventor after: Xiao Xiang

Inventor after: Hu Lei

Inventor after: Xie Dongrong

Inventor after: Xu Qing

Inventor after: Zhou Shiquan

Inventor after: Rao Ronghui

Inventor after: Qiu Huiming

Inventor after: Huang Jiping

Inventor after: Ma Miaoming

Inventor after: Chen Liufeng

Inventor before: Deng Yun

Inventor before: Huang Jiping

Inventor before: Xu Qing

Inventor before: Qiu Huiming

Inventor before: Ma Miaoming

Inventor before: Hu Zhongquan

GR01 Patent grant
GR01 Patent grant
EE01 Entry into force of recordation of patent licensing contract
EE01 Entry into force of recordation of patent licensing contract

Application publication date: 20150121

Assignee: Fujian Nanping Youran Agricultural Comprehensive Development Co.,Ltd.

Assignor: FUJIAN NANPING AGRICULTURAL SCIENCE INSTITUTE

Contract record no.: X2023350000107

Denomination of invention: A method of artificially inducing the natural occurrence of wheat scab

Granted publication date: 20170704

License type: Common License

Record date: 20230323

Application publication date: 20150121

Assignee: Mount Wuyi Daoxiang Ecological Agricultural Technology Development Co.,Ltd.

Assignor: FUJIAN NANPING AGRICULTURAL SCIENCE INSTITUTE

Contract record no.: X2023350000108

Denomination of invention: A method of artificially inducing the natural occurrence of wheat scab

Granted publication date: 20170704

License type: Common License

Record date: 20230323

Application publication date: 20150121

Assignee: Fujian Wotian Jiahe Agricultural Development Co.,Ltd.

Assignor: FUJIAN NANPING AGRICULTURAL SCIENCE INSTITUTE

Contract record no.: X2023350000104

Denomination of invention: A method of artificially inducing the natural occurrence of wheat scab

Granted publication date: 20170704

License type: Common License

Record date: 20230323

Application publication date: 20150121

Assignee: Nanping Jianyang Hetianxia Agricultural Development Co.,Ltd.

Assignor: FUJIAN NANPING AGRICULTURAL SCIENCE INSTITUTE

Contract record no.: X2023350000105

Denomination of invention: A method of artificially inducing the natural occurrence of wheat scab

Granted publication date: 20170704

License type: Common License

Record date: 20230323

Application publication date: 20150121

Assignee: Mount Wuyi Kelixing Seed Industry Co.,Ltd.

Assignor: FUJIAN NANPING AGRICULTURAL SCIENCE INSTITUTE

Contract record no.: X2023350000106

Denomination of invention: A method of artificially inducing the natural occurrence of wheat scab

Granted publication date: 20170704

License type: Common License

Record date: 20230323

EE01 Entry into force of recordation of patent licensing contract
EE01 Entry into force of recordation of patent licensing contract

Application publication date: 20150121

Assignee: Fujian Nanping Julong Ecological Development Co.,Ltd.

Assignor: FUJIAN NANPING AGRICULTURAL SCIENCE INSTITUTE

Contract record no.: X2023350000143

Denomination of invention: A method of artificially inducing the natural occurrence of wheat scab

Granted publication date: 20170704

License type: Common License

Record date: 20230328

Application publication date: 20150121

Assignee: WUYISHAN BANMU FANGTANG ECOLOGICAL AGRICULTURE CO.,LTD.

Assignor: FUJIAN NANPING AGRICULTURAL SCIENCE INSTITUTE

Contract record no.: X2023350000142

Denomination of invention: A method of artificially inducing the natural occurrence of wheat scab

Granted publication date: 20170704

License type: Common License

Record date: 20230328

EE01 Entry into force of recordation of patent licensing contract
EE01 Entry into force of recordation of patent licensing contract

Application publication date: 20150121

Assignee: Nanping Jianyang Dongshanlong Ecological Agriculture Development Co.,Ltd.

Assignor: FUJIAN NANPING AGRICULTURAL SCIENCE INSTITUTE

Contract record no.: X2023350000288

Denomination of invention: A method of artificially inducing Spontaneous generation occurrence of wheat scab

Granted publication date: 20170704

License type: Common License

Record date: 20230619

Application publication date: 20150121

Assignee: Nanping Jianyang Xiaofu Seed Co.,Ltd.

Assignor: FUJIAN NANPING AGRICULTURAL SCIENCE INSTITUTE

Contract record no.: X2023350000289

Denomination of invention: A method of artificially inducing Spontaneous generation occurrence of wheat scab

Granted publication date: 20170704

License type: Common License

Record date: 20230619