CN104115673B - A kind of breeding method viewing and admiring Ganoderma - Google Patents
A kind of breeding method viewing and admiring Ganoderma Download PDFInfo
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- CN104115673B CN104115673B CN201410388265.3A CN201410388265A CN104115673B CN 104115673 B CN104115673 B CN 104115673B CN 201410388265 A CN201410388265 A CN 201410388265A CN 104115673 B CN104115673 B CN 104115673B
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Abstract
The present invention relates to a kind of breeding method viewing and admiring Ganoderma, it is characterized in that using choosing to set → chop at a tree → technological process of intercept → bag → sterilizing → inoculation → bacterium → urge flower bud → educate sesame → moulding, with pure broad leaf tree basswood as material, select and preferably view and admire lucidum variety, make Liquid Strain of Ganoderma Lucidum, use the inoculation of liquid-spawn inoculation method.Bacterium, Ganoderma sporophore growth and development stage is sent out Ganoderma, the temperature of comprehensive regulation environment, humidity, illumination and gas concentration lwevel, in sesame flower bud idiophase, sesame handle elongating stage and Zhi Gai idiophase, utilize the phototaxis of Ganoderma, reproducibility and the biological characteristics of the sensitivity to carbon dioxide, take Ganoderma live body grafting, light source induction, the key technology of sesame lid regeneration respectively, carry out live body Ganoderma design, cultivate that implied meaning is lucky, the live body Ganoderma of great ornamental value.The Ganoderma natural, ecological of cultivation, wonderful workmanship excelling nature, showing artistic form and the cultural connotation of Ganoderma uniqueness, the Ganoderma of cultivation both can become the noble gift of presenting friends, it is possible to as art collection at home, have broad application prospects, also will produce good economic benefit and social benefit.
Description
Technical field
The invention belongs to fungus field, be specifically related to a kind of breeding method viewing and admiring Ganoderma.
Background technology
It is subordinate to Basidiomycotina, Hymenomycetes, Aphyllophorales, Ganodermataceae, Ganoderma Ganoderma.It is commonly called as Ganoderma lucidum seu Japonicum, Ganoderma, Herba mesonae chinensis, Ganoderma, 10000 years bacterium.The Chinese nation is the country recognizing, study, apply Ganoderma the most the earliest, and create show unique characteristics Chinese Ganoderma culture, the image of Ganoderma also become have good luck, auspicious auspicious symbol of blessing.The magical effect such as " strengthening the body resistance " that Shennong's Herbal, Compendium of Material Medica etc. are narrated, " long eclipse duration is raw " is the most widely known, be handed down from age to age.Exploitation implied meaning auspicious sign, show taste, the live body of Fresh and elegant views and admires Ganoderma, is that market potential is huge, development prospect is wide.
In prior art, using the Ganoderma that conventional method is cultivated, live body Ganoderma trophophase is short, it is not easy to preserving, and sesame handle is the shortest, branch is few, and level is poor, does not have sight.For solving the problems referred to above, it is proposed that the present invention.
Summary of the invention
It is an object of the invention to provide a kind of live body of cultivating and view and admire Ganoderma method, the live body Ganoderma of cultivation is simple and unsophisticated pure and fresh, and scene is attractive in appearance, great ornamental value, is suitable to household, office space etc. indoor as decoration, and the most also there is reserve value pole.
For achieving the above object, the present invention adopts the following technical scheme that
A kind of breeding method viewing and admiring Ganoderma, it is characterized in that using choosing to set → chop at a tree → technological process of intercept → bag → sterilizing → inoculation → bacterium → urge flower bud → educate sesame → moulding, with pure broad leaf tree basswood as material, select and preferably view and admire lucidum variety, make liquid spawn, use liquid-spawn inoculation, bacterium and Ganoderma growth stage is sent out Ganoderma, the temperature of comprehensive regulation environment, humidity, illumination and gas concentration lwevel, in the sesame flower bud idiophase, sesame handle elongating stage, the sesame lid idiophase, utilize the phototaxis of Ganoderma, reproducibility and the biological characteristics of the sensitivity to carbon dioxide, take Ganoderma live body grafting respectively, light source is induced, the key technology of sesame lid regeneration, carry out live body Ganoderma cultivation and design, cultivate implied meaning lucky, the live body Ganoderma of great ornamental value.
It is an object of the invention to provide a kind of method that Ganoderma is viewed and admired in cultivation, the Ganoderma natural, ecological of cultivation, wonderful workmanship excelling nature, show again design and the cultural connotation of Ganoderma uniqueness, the live body Ganoderma cultivated both can become the noble gift of presenting friends, also can be as artistic decoration at home, the most great reserve value simultaneously.
For achieving the above object, the present invention adopts the following technical scheme that
Choosing is used to set → chop at a tree → technological process of intercept → bag → sterilizing → inoculation → bacterium → urge flower bud → educate sesame → moulding, with pure broad leaf tree basswood as material, select and preferably view and admire lucidum variety, use liquid-spawn inoculation, bacterium and Ganoderma growth stage is sent out Ganoderma, the temperature of comprehensive regulation environment, humidity, illumination and gas concentration lwevel, in the sesame flower bud idiophase, sesame handle elongating stage, the sesame lid idiophase, utilize the phototaxis of Ganoderma, reproducibility and the biological characteristics of the sensitivity to carbon dioxide, take Ganoderma live body grafting respectively, light source is induced, the key technology of sesame lid regeneration, carry out live body and view and admire cultivation and the design of Ganoderma.
It specifically comprises the following steps that
Choosing tree: select sweet oak tree, evergreen chinquapin, maple, Qinggang oak deciduous species；
Chop at a tree: before inoculation, 20d chops at a tree, be dried 20d under natural conditions to water content 35%～40%；
Intercept: basswood is blocked the short basswood of a length of 15cm～25cm；
Bag: according to the size of basswood diameter, select the polyethylene plastic bag pack in the flat footpath of different size；
Sterilizing: use normal-pressure sterilization method sterilizing；
Inoculation: select Ganoderma lucidum (Leyss. Ex Fr.) Karst. and Ganoderma kind, makes liquid spawn, takes liquid-spawn inoculation method to inoculate；
Send out bacterium: pocket moving into after inoculation culturing room and cultivates, culturing room keeps dark, and temperature controls at 25 DEG C～28 DEG C, relative air humidity is maintained at 60%～70%, ventilates every day 2 times～3 times, each 30min～40min, after mycelia covers with bacterium outer surface of wood layer, loosen the jag of bag mouth in order to ventilative；
Urge flower bud: when bacterium wood surface starts to be transferred to by white faint yellow and when there is the former base of white warty, being moved into by bacterium wood and educate sesame room, now, by the bag mouth warp of bacterium wood, bag mouth is away from bacterium wood section about 2cm.Educate sesame room temperature and control at 30 DEG C～32 DEG C, relative air humidity 90%～95%, intensity of illumination 2000Lux-2500Lux, ventilate every day 2 times～3 times, each 20min～30min, promote former base to develop into Zhi Lei；
Educating sesame: carry out dredging flower bud before Zhi Lei is divided into sesame handle, stay 1～3 sesame flower buds in one end of each bacterium wood, what basis was viewed and admired simultaneously needs to carry out live body Ganoderma graft modeling.During Zhi Lei protrudes upward formation sesame handle, adjustment intensity of illumination is 500Lux-1000Lux, reduce and ventilate, ventilate every day 1 time, each 10min～20min, improves and educates sesame room gas concentration lwevel, educate sesame room temperature and control at 30 ° of C～32 ° of C, relative air humidity controls 90%～95%, suppression sesame lid differentiation, promotes handle length length and length thick.When sesame handle length to 7 cm～12 cm, keep educating sesame room temperature at 30 ° of C～32 ° of C, relative air humidity 90%～95%, intensity of illumination increases to 2000Lux～2500 Lux, and keep educating sesame room uniform light, it is ensured that sesame lid grows the most in the horizontal direction, ventilates every day 2 times～3 times, 20min～30min, promotes sesame lid to break up every time.Live body Ganoderma grafting, light source induction, the key technology of sesame lid regeneration is taked to carry out live body Ganoderma design respectively in sesame flower bud idiophase, sesame handle elongating stage, sesame lid idiophase.When Zhi Gai edge white ring complete yellowing ring and sesame lid no longer increase, Ganoderma growth and maturity, the plastic bag being enclosed within outside bacterium wood can be sloughed, keep bacterium wood surface to be dried, for viewing and admiring and collecting.
The innovation point of the present invention:
A. using pure basswood is culture materials, draws materials abundant.Without special facilities and equipment, investment can greatly can, easy and simple to handle, easily implement, can be with large-scale promotion application；
B. using liquid-spawn inoculation, growth cycle is short, and success ratio of inoculation is high, work efficiency is high；
C. utilizing bacterium wood to delay the feature that controlled release is supported, the Ganoderma of cultivation is live body, the most older 1 batch～2 batches of Ganodermas, Second Year, within the 3rd year, takes the same technical management can be with continued growth Ganoderma on basswood, and it is long that the live body Ganoderma trophophase of cultivation is available for the time of viewing and admiring；
D. making full use of the biological characteristics that Ganderma lucidum is unique, take the combination of modern biotechnology and traditional handicraft, the ganoderma lucidum product unity of form and spirit, the attitude that cultivate are multifarious, great ornamental value and reserve value.
Detailed description of the invention:
A kind of breeding method viewing and admiring Ganoderma, is characterized in that using choosing to set → chop at a tree → technological process of intercept → bag → sterilizing → inoculation → bacterium → urge flower bud → educate sesame → moulding；With pure broad leaf tree basswood as material, select the lucidum variety preferably viewed and admired, make Liquid Strain of Ganoderma Lucidum, use the inoculation of liquid-spawn inoculation method；Bacterium and Ganoderma sporophore growth and development stage, the temperature of comprehensive regulation environment, humidity, illumination and gas concentration lwevel is sent out Ganoderma；In sesame flower bud idiophase, sesame handle elongating stage and Zhi Gai idiophase, utilize the phototaxis of Ganoderma, reproducibility and the biological characteristics of the sensitivity to carbon dioxide, take Ganoderma live body grafting, light source induction, the key technology of sesame lid regeneration respectively, carry out cultivation and the design of live body Ganoderma, cultivate that implied meaning is lucky, the live body Ganoderma of great ornamental value.
Select sweet oak tree, evergreen chinquapin, maple, Qinggang oak deciduous species.Before inoculation, 20d-25d chops at a tree, and is dried about 20d to water content 35%～40% under natural conditions.Suitable broad leaf tree basswood is blocked the short basswood of a length of 15cm～25cm.According to the size of basswood diameter, select the polyethylene plastic bag pack in the flat footpath of different size.Use conventional atmospheric sterilizing methods.Color and form is selected to have Ganoderma lucidum (Leyss. Ex Fr.) Karst. and the Ganoderma kind of enjoying value.Make Liquid Strain of Ganoderma Lucidum, take liquid-spawn inoculation method to inoculate.Pocket moving into after inoculation culturing room cultivate, culturing room keeps dark, and temperature controls at 25 DEG C～28 DEG C, relative air humidity is maintained at 60%～70%, ventilates every day 2 times～3 times, each 30min～40min, after mycelia covers with bacterium outer surface of wood layer, loosen the jag of bag mouth in order to ventilative.When bacterium wood surface starts to be transferred to by white faint yellow and when there is the former base of white warty, bacterium wood is moved into and educates sesame room, bag mouth warp bacterium wood, bag mouth, away from bacterium wood section about 2cm, is educated sesame room temperature and is controlled at 30 DEG C～32 DEG C, relative air humidity 90%～95%, intensity of illumination 2000Lux-2500Lux, ventilate every day 2 times～3 times, each 20min～30min, promote former base to develop into Zhi Lei.Carry out dredging flower bud before Zhi Lei is divided into sesame handle, stay 1～3 sesame flower buds in one end of each bacterium wood.During Zhi Lei protrudes upward formation sesame handle, adjusting intensity of illumination is 500 Lux
-1000Lux, reduces and ventilates, ventilate every day 1 time～2 times, 10min～20min every time, improves and educates sesame room gas concentration lwevel, educate sesame room temperature and control at 30 ° of C～32 ° of C, relative air humidity controls 90%～95%, suppression sesame lid differentiation, promotes handle length length and length thick.When sesame handle length to 7 cm～12 cm, keep educating sesame room temperature at 30 ° of C～32 ° of C, relative air humidity 90%～95%, intensity of illumination increases to 2000Lux～3000 Lux, preferably 2000-2500Lux, and keeps educating sesame room uniform light, ensure that sesame lid grows the most in the horizontal direction, ventilate every day 2 times～3 times, each 20min～30min, promote sesame lid to break up.Take the key technologies such as Ganoderma live body grafting, light source induction, sesame lid regeneration respectively in sesame flower bud idiophase, sesame handle elongating stage, sesame lid idiophase, carry out the design such as live body Ganoderma grafting, sesame handle bending stretching, extension, sesame lid regeneration.When Zhi Gai edge white ring complete yellowing ring and sesame lid no longer increase, Ganoderma growth and maturity, the plastic bag being enclosed within outside bacterium wood can be sloughed, keep bacterium wood surface to be dried, for viewing and admiring and collecting.
The design technical measures of live body Ganoderma:
Live body Ganoderma graft modeling: according to moulding needs, on sesame flower bud to be grafted, first cut an otch or cut next section, reselection stalwartness and the most undifferentiated scion of sesame lid, the sharp cutter sterilized with ethanol gets off along base portion truncation, in rapidly scion is inserted the otch of sesame flower bud to be grafted or be pasted onto on the tangent plane of sesame flower bud to be grafted, keep the seamless stickup of grafting point as far as possible, accelerate the mycelia healing of otch.After grafting completes, being moved into by grafting bacterium wood and educate sesame room, educate sesame room temperature and control at 26 ° of C～28 ° of C, relative air humidity controls 90%～95%, ventilates every day 2 times～3 times, each 20min～30min.Forbidding within 2d～3d after grafting spraying water on Xiang Zhiti, after 2d～3d, at grafting, mycelia has recovered growth, and scion and grafting sesame flower bud continue to be divided into sesame lid, the Ganoderma moulding come in every shape.
Live body Ganoderma sesame shank shape moulding: during Zhi Lei protrudes upward formation sesame handle, stretch bacterium bag bag mouth, and on bacterium bag, instead overlap a lighttight paper web, paper web is opened the aperture of a diameter 2cm, the sesame handle intensity of illumination giving to intend induction by aperture is 500Lux～the irradiation of 1000 Lux light sources, educate sesame room temperature and control at 30 ° of C～32 ° of C, relative air humidity controls 90%～95%, induction sesame handle bends strongly to light source direction, then sleeve is removed, promote sesame lid to continue differentiation, form the Ganoderma moulding that sesame shank shape is different.
Sesame covers regeneration live body Ganoderma moulding: in sesame lid atomization, the bacterium bag of growth children sesame is stretched bag mouth, one lighttight paper web of anti-set on bag mouth, the bacterium bag mouth stretched and paper web are opened the aperture of an a diameter of 2cm, give intensity of illumination 2000Lux～the Zhi Gai edge of 3000Lux illumination direct projection plan moulding children sesame, educate sesame room temperature and control at 30 ° of C～32 ° of C, relative air humidity 90%～95%, ventilate every day 2 times～3 times, 20min～30min every time, after 2d～3d, new former base is differentiated at the Zhi Gaichu of printing opacity, former base develops into Zhi Lei, Zhi Lei stretches out into sesame handle, then sleeve is removed, sesame lid is promoted to continue differentiation, sesame covers regeneration live body Ganoderma.
Live body Ganoderma two-sided sesame lid moulding: break up the later stage at sesame lid, Zhi Gai edge is also put in the Ganoderma opposite direction of differentiation, give Zhi Gai back side 2000Lux～3000 Lux, preferably 2000Lux～the illumination of 2500 Lux, educate sesame room temperature and control at 30 ° of C～32 ° of C, relative air humidity controls 90%～95%, ventilates every day 2 times～3 times, each 20min～30min.After 3d～5d, breaking up the former base made new advances at the Zhi Gai back side of illumination, former base constantly breaks up, until growing up to the Ganoderma of two-sided sesame lid.
Embodiment 1
Select sweet oak tree as cultivating material.Before inoculation, 20d chops at a tree, and is dried about 22d to water content about 40% under natural conditions.Sweet oak tree basswood is blocked the short basswood of a length of 15cm.According to the size of basswood diameter, select the polyethylene plastic bag pack in the flat footpath of different size.Use normal-pressure sterilization method sterilizing.Select Ganoderma lucidum (Leyss. Ex Fr.) Karst. kind.Make Liquid Strain of Ganoderma Lucidum, take liquid-spawn inoculation method to inoculate.After inoculation, bacterium bag being moved into culturing room to cultivate, culturing room keeps dark, and temperature controls at 26 DEG C, and relative air humidity keeps 70%, ventilates every day 2 times, each 30min, and after mycelia covers with bacterium outer surface of wood layer, the jag loosening bag mouth is breathed freely.When bacterium wood surface starts to be transferred to by white faint yellow and when there is the former base of white warty, being moved into by bacterium wood and educate sesame room, by the bag mouth warp of bacterium wood, bag mouth is away from bacterium wood section about 2cm.Educate sesame room temperature and control at 32 DEG C, relative air humidity 90%, intensity of illumination 2000Lux, ventilate every day 2 times, each 30min.Carry out dredging flower bud before Zhi Lei is divided into sesame handle, stay 1 sesame flower bud in one end of each bacterium wood.During Zhi Lei protrudes upward formation sesame handle, adjusting intensity of illumination is 500 Lux, ventilates every day 1 time, and each 10min improves and educates sesame room gas concentration lwevel, educates sesame room temperature and control at 30 ° of C, and relative air humidity controls 90%.When sesame handle length is to 7 cm, keeps educating sesame room temperature and increase to 2000Lux, and keep educating sesame room uniform light in 30 ° of C, relative air humidity 90%, intensity of illumination, ventilate every day 2 times, each 20min.Graft modeling: according to moulding needs, on sesame flower bud to be grafted, first cut an otch or cut next section, reselection stalwartness and the most undifferentiated scion of sesame lid, the sharp cutter sterilized with ethanol gets off along base portion truncation, in rapidly scion is inserted the otch of sesame flower bud to be grafted or be pasted onto on the tangent plane of sesame flower bud to be grafted, keep the seamless stickup of grafting point as far as possible, accelerate the mycelia healing of otch.After grafting completes, being moved into by grafting bacterium wood and educate sesame room, educate sesame room temperature and control at 26 ° of C, relative air humidity controls 90%, ventilates every day 2 times, each 20min.Forbidding within 2d～3d after grafting spraying water on Xiang Zhiti, after 2d～3d, at grafting, mycelia has recovered growth, and scion and grafting sesame flower bud continue to be divided into sesame lid, the Ganoderma moulding come in every shape.When Zhi Gai edge white ring complete yellowing ring and sesame lid no longer increase, Ganoderma growth and maturity, the plastic bag being enclosed within outside bacterium wood can be sloughed, keep bacterium wood surface to be dried, for viewing and admiring and collecting.
Example 2
Select evergreen chinquapin seeds for cultivating material.Before inoculation, 22d chops at a tree, and is dried 20d to water content 36% under natural conditions.Basswood is blocked the short basswood of a length of 18cm.According to the size of basswood diameter, select polyethylene plastic bag pack.Use normal-pressure sterilization method sterilizing.Select Ganoderma lucidum (Leyss. Ex Fr.) Karst. kind.Make liquid spawn, take liquid-spawn inoculation method to inoculate.Pocket moving into after inoculation culturing room cultivate, culturing room keeps dark, and temperature controls at 27 DEG C, and relative air humidity is maintained at 68%, ventilates every day 2 times, each 25min.After mycelia covers with bacterium outer surface of wood layer, loosen the jag of bag mouth.When bacterium wood surface starts to be transferred to by white faint yellow and when there is the former base of white warty, bacterium wood is moved into and educates sesame room.The bag mouth warp of bacterium wood, bag mouth, away from bacterium wood section about 2cm, is educated sesame room temperature and is controlled at 30 DEG C, relative air humidity 92%, intensity of illumination 2200Lux.Ventilate every day 2 times, each 25min.Carry out dredging flower bud before Zhi Lei is divided into sesame handle, stay 2 sesame flower buds in one end of each bacterium wood.During Zhi Lei protrudes upward formation sesame handle, adjusting intensity of illumination is 600 Lux
, reduce and ventilate, ventilate every day 1 time, each 15min, improve and educate sesame room gas concentration lwevel, educate sesame room temperature and control at 30 ° of C, relative air humidity controls 92%.When sesame handle length is to 10 cm, keeps educating sesame room temperature and increase to 2500Lux in 30 ° of C, relative air humidity 92%, intensity of illumination, and keep educating sesame room uniform light, it is ensured that sesame lid grows the most in the horizontal direction, ventilates every day 2 times, each 25min.Live body Ganoderma sesame shank shape moulding: during Zhi Lei protrudes upward formation sesame handle, stretch bacterium bag bag mouth, and on bacterium bag, instead overlap a lighttight paper web, paper web is opened the aperture of a diameter 2cm, the sesame handle intensity of illumination giving to intend induction by aperture is that 800Lux light source irradiates, educate sesame room temperature and control at 30 ° of C, relative air humidity controls 90%, induction sesame handle bends strongly to light source direction, then sleeve is removed, promote sesame lid to continue differentiation, form the Ganoderma moulding that sesame shank shape is different.When Zhi Gai edge white ring complete yellowing ring and sesame lid no longer increase, Ganoderma growth and maturity, the plastic bag being enclosed within outside bacterium wood can be sloughed, keep bacterium wood surface to be dried, for viewing and admiring and collecting.
Example 3
Select maple as cultivating material.Before inoculation, 25d chops at a tree, and is dried 22d to water content 38% under natural conditions.Maple basswood is blocked the short basswood of a length of 20cm.According to the size of basswood diameter, select the polyethylene plastic bag pack in the flat footpath of different size.Use normal-pressure sterilization method sterilizing.Select Ganoderma kind.Make Liquid Strain of Ganoderma Lucidum, inoculate according to liquid-spawn inoculation method.Pocket moving into after inoculation culturing room cultivate, culturing room keeps dark, and temperature controls at 26 DEG C, and relative air humidity is maintained at 65%, ventilates every day 3 times, each 35min.After mycelia covers with bacterium outer surface of wood layer, the jag loosening bag mouth is breathed freely.When bacterium wood surface starts to be transferred to by white faint yellow and when there is the former base of white warty, bacterium wood is moved into and educates sesame room.The bag mouth warp of bacterium wood, bag mouth is away from bacterium wood section about 2cm.Educating sesame room temperature and control at 32 DEG C, relative air humidity controls 92%, intensity of illumination 2400Lux, ventilates every day 3 times, each 30min.Carry out dredging flower bud before Zhi Lei is divided into sesame handle, stay 2 sesame flower buds in one end of each bacterium wood.During Zhi Lei protrudes upward formation sesame handle, adjusting intensity of illumination is 800 Lux
, reduce and ventilate, ventilate every day 2 times, each 15min, improve and educate sesame room gas concentration lwevel, educate sesame room temperature and control at 32 ° of C, relative air humidity controls 92%.When sesame handle length is to 10cm, keep educating sesame room temperature at 32 ° of C, relative air humidity 92%, intensity of illumination increases to 2500 Lux, and keeps educating sesame room uniform light, it is ensured that sesame lid grows the most in the horizontal direction, ventilate every day 3 times, each 30min, promote sesame lid to break up.Sesame covers regeneration live body Ganoderma moulding: in sesame lid atomization, the bacterium bag of growth children sesame is stretched bag mouth, one lighttight paper web of anti-set on bag mouth, the bacterium bag mouth stretched and paper web are opened the aperture of an a diameter of 2cm, give intensity of illumination 2000Lux～the Zhi Gai edge of 3000Lux illumination direct projection plan moulding children sesame, educate sesame room temperature and control at 30 ° of C～32 ° of C, relative air humidity 90%～95%, after 2d～3d, new former base is differentiated at the Zhi Gaichu of printing opacity, former base develops into Zhi Lei, Zhi Lei stretches out into sesame handle, then sleeve is removed, sesame lid is promoted to continue differentiation, sesame covers regeneration live body Ganoderma.When Zhi Gai edge white ring complete yellowing ring and sesame lid no longer increase, Ganoderma growth and maturity, the plastic bag being enclosed within outside bacterium wood can be sloughed, keep bacterium wood surface to be dried, for viewing and admiring and collecting.
Example 4
Select Qinggang robur kind as cultivating material.Before inoculation, 25d chops at a tree, and is dried 25d to water content 40% under natural conditions.Qinggang oak basswood is blocked the short basswood of a length of 25cm.According to the size of basswood diameter, select the polyethylene plastic bag pack in the flat footpath of different size.Use normal-pressure sterilization method sterilizing.Select Ganoderma lucidum (Leyss. Ex Fr.) Karst. kind.Make Liquid Strain of Ganoderma Lucidum, inoculate according to liquid-spawn inoculation method.Pocket moving into after inoculation culturing room cultivate, culturing room keeps dark, and temperature controls at 28 DEG C, and relative air humidity is maintained at 70%, ventilates every day 3 times, each 30min, and after mycelia covers with bacterium outer surface of wood layer, the jag loosening bag mouth is breathed freely.When bacterium wood surface starts to be transferred to by white faint yellow and when there is the former base of white warty, bacterium wood is moved into and educates sesame room.The bag mouth warp of bacterium wood, bag mouth is away from bacterium wood section about 2cm.Educating sesame room temperature and control at 30 DEG C, relative air humidity controls 95%, and intensity of illumination controls at 2500Lux, ventilates every day 3 times, and each 25min promotes former base to develop into Zhi Lei.Carry out dredging flower bud before Zhi Lei is divided into sesame handle, stay 3 sesame flower buds in one end of each bacterium wood.During Zhi Lei protrudes upward formation sesame handle, adjustment intensity of illumination is 1000Lux, ventilates every day 1 time, each 20min, educates sesame room temperature and controls at 28 ° of C, and relative air humidity controls 90%.When sesame handle length is to 12 cm, keep educating sesame room temperature at 32 ° of C, relative air humidity 90%, intensity of illumination increases to 2800 Lux, and keeps educating sesame room uniform light, it is ensured that sesame lid grows the most in the horizontal direction, ventilate every day 3 times, each 20min, promote sesame lid to break up.Live body Ganoderma two-sided sesame lid moulding: break up the later stage at sesame lid, also puts Zhi Gai edge, gives the illumination of the Zhi Gai back side 3000 Lux in the Ganoderma opposite direction of differentiation, educate sesame room temperature and control at 30 ° of C, relative air humidity controls 90%, ventilates every day 2 times, each 30min.After 3d～5d, breaking up the former base made new advances at the Zhi Gai back side of illumination, former base constantly breaks up, until growing up to the Ganoderma of two-sided sesame lid.When Zhi Gai edge white ring complete yellowing ring and sesame lid no longer increase, Ganoderma growth and maturity, the plastic bag being enclosed within outside bacterium wood can be sloughed, keep bacterium wood surface to be dried, for viewing and admiring and collecting.
Claims (5)
1. view and admire a breeding method for Ganoderma, it is characterized in that using choosing tree → a chop at a tree → intercept → bag → sterilizing → inoculation → bacterium → urge flower bud → educate sesame
The technological process of → moulding；With pure broad leaf tree basswood as material, select the lucidum variety preferably viewed and admired, make Liquid Strain of Ganoderma Lucidum, use liquid bacteria
Plant inoculation method inoculation；Bacterium and Ganoderma sporophore growth and development stage, the temperature of comprehensive regulation environment, humidity, illumination and titanium dioxide is sent out Ganoderma
Concentration of carbon；In sesame flower bud idiophase, sesame handle elongating stage and Zhi Gai idiophase, utilize the phototaxis of Ganoderma, reproducibility and the sensitivity to carbon dioxide
Biological characteristics, takes Ganoderma live body grafting, light source induction, the key technology of sesame lid regeneration respectively, and the cultivation and the art that carry out live body Ganoderma are made
Type, cultivates that implied meaning is lucky, the live body Ganoderma of great ornamental value, wherein: choosing tree: select deciduous species sweet oak tree, evergreen chinquapin, maple, Qinggang
Oak is as cultivating material；
Chop at a tree: before inoculation, about 20d chops at a tree, be dried about 20d under natural conditions, to water content 35%～40%；
Intercept: basswood is blocked the short basswood of a length of 15cm～25cm；
Bag: according to the size of basswood diameter, select the polyethylene plastic bag pack in the flat footpath of different size；
Sterilizing: use normal-pressure sterilization method sterilizing；
Inoculation: select Ganoderma lucidum (Leyss. Ex Fr.) Karst. and Ganoderma kind as cultivating kind, make Liquid Strain of Ganoderma Lucidum, inoculate according to liquid-spawn inoculation method；
Sending out bacterium: pocket moves into after inoculation culturing room and cultivates, the nurturing period, culturing room keeps dark, and temperature controls at 25 DEG C～28 DEG C, and air is relative
Humidity is maintained at 60%～70%, ventilates every day 2 times～3 times, and each 30min～40min, after mycelia covers with bacterium outer surface of wood layer, loosens bag mouth
Jag is in order to ventilative；
Urge flower bud: when bacterium wood two end surfaces start to be transferred to by white faint yellow and when there is the former base of white warty, the immigration of bacterium wood is educated sesame room, now, by bacterium
The bag mouth warp of wood, bag mouth, away from bacterium wood section about 2cm, educates sesame room temperature and controls at 30 DEG C～32 DEG C, relative air humidity control 90%～
95%, intensity of illumination controls at 2000Lux-2500Lux, ventilates every day 2 times～3 times, and each 20min～30min promotes former base to develop into
Zhi Lei；
Educate sesame: carry out dredging flower bud before Zhi Lei is divided into sesame handle, stay 1～3 healthy and strong sesame flower bud in one end of each bacterium wood, protrude upward formation at Zhi Lei
During sesame handle, educate sesame room intensity of illumination and be adjusted to 500Lux-1000Lux, reduce and ventilate, ventilate every day 1 time, each 10min～20min,
Improving and educate sesame room gas concentration lwevel, educate sesame room temperature and control at 30 DEG C～32 DEG C, relative air humidity controls 90%～95%, suppression
Sesame lid breaks up, and promotes handle length length and long thick, when sesame handle length is to 7cm～12cm, keeps educating sesame room temperature at 30 DEG C～32 DEG C, air
Relative humidity 90%～95%, intensity of illumination increases to 2000Lux～2500Lux, and keeps educating sesame room uniform light, it is ensured that sesame lid uniformly exists
Horizontal direction grows, and ventilates every day 2 times～3 times, and each 20min～30min promotes sesame lid to break up；
Live body Ganoderma moulding: in sesame flower bud idiophase, sesame handle elongating stage, sesame lid idiophase, take Ganoderma live body grafting, light source induction, sesame lid respectively again
Raw key technology, carries out cultivation and the design of live body Ganoderma；
When Zhi Gai edge white ring complete yellowing ring and sesame lid no longer increase, Ganoderma growth and maturity, the plastics being enclosed within outside bacterium wood can be sloughed
Bag, keeps bacterium wood surface to be dried, for viewing and admiring and collecting.
The breeding method viewing and admiring Ganoderma the most according to claim 1, carries out live body Ganoderma sesame shank shape moulding: protrude upward at Zhi Lei and form sesame
During handle, educating sesame room temperature and control at 30 DEG C～32 DEG C, relative air humidity controls 90%～95%, stretches bacterium bag bag mouth, and
On bacterium bag, one lighttight paper web of anti-set, opens the aperture of a diameter 2cm on paper web, gives to intend the sesame handle illumination of induction by aperture
Intensity is that 500Lux～1000Lux light source irradiates, and induction sesame handle bends strongly to light source direction, forms the sesame shank shape of different modeling.
The breeding method viewing and admiring Ganoderma the most according to claim 1, carries out live body Ganoderma graft modeling: according to moulding needs, first to be grafted
Cutting an otch on Zhi Lei or cut next section, reselection is healthy and strong and the most undifferentiated scion of sesame lid, with the sterilized sharp cutter of ethanol along base portion
Truncation is got off, and in scion is inserted rapidly the otch of sesame flower bud or be pasted onto on the tangent plane of sesame flower bud, keeps the seamless stickup of grafting point as far as possible, accelerates to cut
The mycelia healing of mouth, after grafting completes, moves into grafting bacterium wood and educates sesame room, educate sesame room temperature and control at 26 DEG C～28 DEG C, and air is the wettest
Degree controls 90%～95%, ventilates every day 2 times～3 times, and each 20min～30min forbids on Xiang Zhiti within 2d～3d after grafting
Water spray, after 2d～3d, inserts at the seam mycelia restoration ecosystem of grafting, Zhi Lei and fringe and continues to be divided into sesame handle and Zhi Gai, live body Ganoderma grafting
Success.
The breeding method viewing and admiring Ganoderma the most according to claim 1, carries out sesame and covers regeneration live body Ganoderma moulding: in sesame lid atomization,
The bacterium bag of growth children sesame stretches bag mouth, and on bag mouth, one lighttight paper web of anti-set, opens an a diameter of 2cm on the bacterium bag mouth stretched and paper web
Aperture, give intensity of illumination 2000Lux～3000Lux illumination direct projection and intend the Zhi Gai edge of moulding children sesame, educate sesame room temperature and control
30 DEG C～32 DEG C, relative air humidity controls 90%～95%, ventilates every day 2 times～3 times, each 20min～30min, 2d～3d
Afterwards, breaking up the former base made new advances in the Zhi Gai edge of printing opacity, former base develops into Zhi Lei, Zhi Lei and stretches out into sesame handle, then removes sleeve,
Promoting sesame lid to continue differentiation, sesame covers regeneration live body Ganoderma.
The breeding method viewing and admiring Ganoderma the most according to claim 1, carries out the two-sided sesame lid moulding of live body Ganoderma: break up the later stage at sesame lid, sesame lid
Edge is also put in the Ganoderma opposite direction of differentiation, gives the illumination of the Zhi Gai back side 2000Lux～2500Lux, educate sesame room temperature control at 30 DEG C～
32 DEG C, relative air humidity controls 90%～95%, ventilates every day 2 times～3 times, each 20min～30min；After 3d～5d,
The Zhi Gai back side of illumination breaks up the former base made new advances, and former base constantly breaks up along sesame lid, until growing up to the Ganoderma of two-sided sesame lid.
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| CN104521557A (en) * | 2014-12-16 | 2015-04-22 | 融水苗族自治县怀宝镇人民政府 | Cultivating method for coralliform lucid ganoderma |
| CN104584861B (en) * | 2015-01-20 | 2016-08-10 | 张振 | The cultivation of a kind of Ganoderma wild mushroom and mould grafting activate shaping methods |
| CN105123270A (en) * | 2015-08-31 | 2015-12-09 | 佛山市高明区更合镇鹏鹄食用菌专业合作社 | Basswood ganoderma lucidum planting method |
| CN105601379A (en) * | 2015-12-23 | 2016-05-25 | 安徽省霍山县轩芝缘生物科技有限公司 | Grafting method of ganoderma lucidum bonsai |
| CN106069206A (en) * | 2016-08-10 | 2016-11-09 | 金祝生 | Indoor log glossy ganoderma pseudo-wild cultivating method |
| CN106538236A (en) * | 2016-10-12 | 2017-03-29 | 重庆市中药研究院 | In the method that Ganoderma prepares health food as raw material |
| CN107079709A (en) * | 2017-03-28 | 2017-08-22 | 云南顶兴农业科技发展有限公司 | A kind of ganoderma lucidum breeding method for viewing and admiring |
| CN108293594A (en) * | 2017-10-20 | 2018-07-20 | 翔天农业开发集团股份有限公司 | A kind of moulding cultural method of ganoderma lucidum landscape type |
| CN110089340A (en) * | 2018-01-31 | 2019-08-06 | 蒋凡 | A kind of method of castanopsis fissa underwood planting ganoderma lucidum |
| CN108990705A (en) * | 2018-08-21 | 2018-12-14 | 巫溪县凤芝农业有限公司 | The method for cultivating cultivating ganoderma based on symbiosis |
| CN108990706A (en) * | 2018-10-23 | 2018-12-14 | 天方健（中国）药业有限公司 | A kind of cultural method of red sesame |
| CN110352793A (en) * | 2019-08-28 | 2019-10-22 | 黑龙江坤健农业股份有限公司 | A kind of ganoderma lucidum rotary planting technology |
| CN112931032A (en) * | 2019-12-11 | 2021-06-11 | 韩淑娜 | Cultivation method of living lucid ganoderma bonsai |
| CN115152525B (en) * | 2021-04-01 | 2023-08-01 | 河源市神农宝灵芝生产经营专业合作社 | Method for cultivating ganoderma lucidum by utilizing illumination |
| CN115176641B (en) * | 2021-04-01 | 2023-08-01 | 河源市神农宝灵芝生产经营专业合作社 | Method for cultivating ganoderma atrum by utilizing illumination |
| CN113615478A (en) * | 2021-08-30 | 2021-11-09 | 武汉生物工程学院 | Preparation method of palm-shaped ganoderma lucidum bonsai |
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| CN1687394A (en) * | 2005-03-31 | 2005-10-26 | 赖潮泰 | Method for planting Ganoderma lucidum |
| CN102067787B (en) * | 2010-11-12 | 2012-03-28 | 安徽黄山云乐灵芝有限公司 | Die and method for directionally culturing glossy ganoderma potted landscapes of various forms |
| CN103026897A (en) * | 2012-12-11 | 2013-04-10 | 昭平县农业局 | Lucid ganoderma bonsai grafting method |
| CN103650829B (en) * | 2013-08-14 | 2015-02-18 | 欧庭武 | Cultivation method of multi-layer purple lucid ganoderma bonsai |
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