CN104054511B - A kind of glossy ganoderma breeding method - Google Patents
A kind of glossy ganoderma breeding method Download PDFInfo
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- CN104054511B CN104054511B CN201410295555.3A CN201410295555A CN104054511B CN 104054511 B CN104054511 B CN 104054511B CN 201410295555 A CN201410295555 A CN 201410295555A CN 104054511 B CN104054511 B CN 104054511B
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Abstract
The invention discloses a kind of glossy ganoderma breeding method, comprise the steps: that medium is prepared--medium pack and sterilizing--the former bacterium of cultivation glossy ganoderma--cultivation cultivation of glossy ganoderma bacterium--inoculation of bacterium rod--cultural hypha-go out sesame management-timely collecting.It is fast that glossy ganoderma cultural method provided by the invention sends out bacterium speed, and cultivation period is short, and cost is low, and the glossy ganoderma a cultivated type is attractive in appearance; In addition, this glossy ganoderma cultural method also avoid the waste to tree resources, takes full advantage of the recycling to crop shell, crop core, stalk and glossy ganoderma slag.
Description
Technical field
The present invention relates to a kind of glossy ganoderma breeding method.
Background technology
Glossy ganoderma is a kind of famous and precious medicinal fungi, through chemical analysis, all containing polysaccharide, several amino acids, organic germanium, steroid class and triterpenes in the real and mycelium of glossy ganoderma, pharmacological research shows, glossy ganoderma has protecting the liver, detoxifies, cardiac stimulant, calm the nerves, pain relieving and control the functions such as tinnitus, beneficial lung qi, sharp joint.
Existing ganoderma lucidum cultivation method mainly contains soil covering culture and substituting stuff cultivation pattern, what the substituting stuff cultivation pattern of current main-stream adopted is diameter is 18cm, length is the cultivation bag of 38cm, and take two or one to inoculate out the mode of sesame, this kind of substituting stuff cultivation pattern not only sends out bacterium speed slowly, also easily causes glossy ganoderma shape distortion irregular.
Summary of the invention
The technical problem to be solved in the present invention is, send out bacterium speed slowly and easily cause the above-mentioned defects such as glossy ganoderma shape distortion is irregular for substituting stuff cultivation pattern in prior art, provide a kind of bacterium speed is fast, cultivation period is short, cost is low and the glossy ganoderma breeding method that the glossy ganoderma a cultivated type is attractive in appearance.
The technical solution adopted for the present invention to solve the technical problems is as follows:
A kind of glossy ganoderma breeding method, comprises the steps:
(1) medium preparation: medium solid portion adopts the raw material of following percentage by weight to obtain: corncob 55-75wt%, soybean stalk 5-20wt%, glossy ganoderma slag 10-25wt%, soya bean soya-bean cake 1-5wt%, sucrose 1-3wt% and land plaster 1-3wt%, and in medium solid portion, add water mix acquisition medium;
(2) medium pack and sterilizing: the medium prepared by step (1) is loaded edible mushroom bag immediately, and the edible mushroom bag after charging is put into atmospheric sterilizing stove and carried out sterilizing;
(3) cultivate the former bacterium of glossy ganoderma: be inoculated in the edible mushroom bag obtained by step (2) by female for glossy ganoderma bacterium, be then placed in aseptic space by postvaccinal edible mushroom bag, make the female bacterium of glossy ganoderma at 20-30
ocarry out growth and breeding at the temperature of C, cultivate burmannaline camphorata mycelium in 20-30 days to edible mushroom bag, namely obtain the former bacterium of glossy ganoderma;
(4) cultivate cultivation of glossy ganoderma bacterium: be inoculated into by the former bacterium of glossy ganoderma obtained by step (3) in the edible mushroom bag obtained by step (2), then the edible mushroom bag of the former bacterium of inoculation glossy ganoderma is placed in aseptic space, makes the former bacterium of glossy ganoderma at 20-30
ocarry out growth and breeding at the temperature of C, cultivate burmannaline camphorata mycelium in 20-30 days to edible mushroom bag, namely obtain cultivation of glossy ganoderma bacterium;
(5) bacterium rod inoculation: adopt Open-Architecture Controller mode, in the inoculation canopy that disinfection is crossed, bacterium rod is beaten inoculation hole successively, then in inoculation hole, inoculates the cultivation of glossy ganoderma bacterium obtained by step (4);
(6) cultural hypha: culturing room will be put into by the postvaccinal bacterium rod of step (5), and the temperature in culturing room is controlled as 24-28
oc, humid control is 50-85%, cultivates and obtains Ganoderma lucidum mycelium in 30-35 days; (7) sesame management is gone out: the Ganoderma lucidum mycelium plantation that will be obtained by step (6), in booth, keeps canopy temperature to be 20-28 in the process of growth of Ganoderma lucidum mycelium
oc, and be 60-95% by the humid control in booth;
(8) timely collecting.
In the step (1) of glossy ganoderma breeding method of the present invention, medium solid portion adopts corncob 55-75wt%, soybean stalk 5-20wt%, glossy ganoderma slag 10-25wt%, soya bean soya-bean cake 1-5wt%, sucrose 1-3wt% and land plaster 1-3wt% proportioning form, and wherein, glossy ganoderma slag refers to that glossy ganoderma extracts rear remaining residue; Existing corncob, soybean stalk and glossy ganoderma slag are all abandoned as refuse or are incinerated, not only cause the waste to the energy, but also can to environment, and using the substrate as medium of corncob, soybean stalk and glossy ganoderma slag in technical scheme of the present invention, namely instead of in prior art the way adopting wood chip as medium substrate, avoid the waste to tree resources, take full advantage of crop shell, crop core, stalk and glossy ganoderma slag, it also avoid environment.
In addition, in above-mentioned medium solid portion, soya bean soya-bean cake is as nitrogenous source, sucrose is as carbon source, and land plaster, as mineral salt, ensure that in above-mentioned medium and is rich in the micro-factor, trophic structure is reasonable, namely reduces the cost of medium, has enriched again substrate trophic structure.
In the step (5) of technical scheme of the present invention, adopt Open-Architecture Controller mode, in the inoculation canopy that disinfection is crossed, bacterium rod is beaten inoculation hole successively, namely instead of the mode of two punching on bacterium rod in prior art, such hole knockout increases quantity bacterium rod being inoculated hole, is of value to improving sending out bacterium speed, thus also contributes to shortening cultivation period and reducing time cost.
In the step (6) of technical scheme of the present invention, postvaccinal bacterium rod is put into culturing room, and the temperature in culturing room is controlled as 24-28
oc, humid control is 50-85%, provides good growth and breeding environment, if temperature is lower than 24 like this to the control of temperature and humidity to cultivation of glossy ganoderma bacterium
oc, or higher than 28
oc, or humidity is lower than 50% or higher than 85%, is all unfavorable for the growth and breeding of cultivation of glossy ganoderma bacterium, therefore under such temperature and humidity condition, only need cultivate and can gather in the crops Ganoderma lucidum mycelium in 30-35 days, shorten the cultivation period of glossy ganoderma to a certain extent.
In the step (7) of technical scheme of the present invention, by Ganoderma lucidum mycelium plantation in booth, and the temperature in booth is controlled as 20-28
oc, humid control is 60-95%, provides good growth and breeding environment like this to the control of temperature and humidity to Ganoderma lucidum mycelium, same as above, if the temperature in booth is lower than 20
oc, or higher than 28
oc, or humidity is lower than 60% or higher than 95%, is all unfavorable for the growth and breeding of Ganoderma lucidum mycelium, under such temperature and humidity condition, only need cultivate glossy ganoderma of can gathering for about 30 days, further shorten the cultivation period of glossy ganoderma.
Improve as to the one of technical scheme of the present invention, in step (1), the humid control of medium is controlled at 5.0-6.0 at 50-60%, pH.
Improve as to the one of technical scheme of the present invention, in step (2), the edible mushroom bag after charging is put into atmospheric sterilizing stove and 95
oc-100
osterilizing 20-24 hour is maintained during C.When in atmospheric sterilizing stove, temperature reaches 95
oc-100
oduring C, maintain sterilizing 20-24 hour, if sterilization time is less than 20 hours, causes sterilizing not thorough possibly, if sterilization time was more than 20 hours, then can cause the waste of time and resource.
Improve as to the one of technical scheme of the present invention, in above-mentioned steps (5), the distance between upper adjacent two the inoculation holes of bacterium rod is 10-12cm.Distance between upper for bacterium rod adjacent two inoculation holes is designed to 10-12cm, increasing on the basis in inoculation hole as far as possible, fully effectively ensure that the growth and breeding space of the cultivation of glossy ganoderma bacterium in each inoculation hole, avoid the cultivation of glossy ganoderma bacterium in adjacent two inoculation holes to interfere with each other, can ensure that a glossy ganoderma type of final results is attractive in appearance to a certain extent.
Improve as to the one of technical scheme of the present invention, the hole depth in inoculation hole is 2-3cm, and the diameter in inoculation hole is 1-1.5cm.The inoculation hole depth in hole and the restriction of diameter, ensure that in each inoculation hole many some cultivation of glossy ganoderma bacterium of inoculation of trying one's best, thus ensure that inoculation survival rate, also improve inoculation speed.
Improve as to the one of technical scheme of the present invention, in step (5), adopt lime to carry out disinfection sterilization to inoculation canopy.Adopting lime to carry out disinfection sterilization to inoculation canopy, production cost be saved when ensureing good sterilization effect.
In addition, in technical scheme of the present invention, to be allly not specifically noted, all by adopting the conventional means in this area to realize the technical program.
Therefore, it is fast that glossy ganoderma cultural method provided by the invention sends out bacterium speed, and cultivation period is short, and cost is low, and the glossy ganoderma a cultivated type is attractive in appearance; In addition, this glossy ganoderma cultural method also avoid the waste to tree resources, takes full advantage of the recycling to crop shell, crop core, stalk and glossy ganoderma slag.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearly understand, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
Embodiment one:
A kind of glossy ganoderma breeding method, comprises the steps:
(1) medium preparation: medium solid portion adopts the raw material of following percentage by weight to obtain: corncob 55wt%, soybean stalk 20wt%, glossy ganoderma slag 20wt%, soya bean soya-bean cake 3wt%, sucrose 1wt% and land plaster 1wt%, and in this medium solid portion, add water mix acquisition medium, now, the humid control of this medium is controlled to be 6.0 at 50%, pH;
(2) medium pack and sterilizing: the medium prepared by step (1) is loaded edible mushroom bag immediately, and the edible mushroom bag after charging is put into atmospheric sterilizing stove and 95
osterilizing 24 hours are maintained during C;
(3) cultivate the former bacterium of glossy ganoderma: be inoculated in the edible mushroom bag obtained by step (2) by female for glossy ganoderma bacterium, be then placed in aseptic space by postvaccinal edible mushroom bag, make the female bacterium of glossy ganoderma 20
ocarry out growth and breeding at the temperature of C, cultivate burmannaline camphorata mycelium in 28 days to edible mushroom bag, namely obtain the former bacterium of glossy ganoderma;
(4) cultivate cultivation of glossy ganoderma bacterium: be inoculated into by the former bacterium of glossy ganoderma obtained by step (3) in the edible mushroom bag obtained by step (2), then the edible mushroom bag of the former bacterium of inoculation glossy ganoderma is placed in aseptic space, makes the former bacterium of glossy ganoderma 20
ocarry out growth and breeding at the temperature of C, cultivate burmannaline camphorata mycelium in 30 days to edible mushroom bag, namely obtain cultivation of glossy ganoderma bacterium;
(5) bacterium rod inoculation: adopt Open-Architecture Controller mode, in the inoculation canopy adopting lime disinfection sterilized, bacterium rod is beaten inoculation hole successively, the distance between adjacent two inoculation holes is 10cm, and the hole depth in inoculation hole is 2cm, the diameter control in inoculation hole is 1cm; Then in inoculation hole, the cultivation of glossy ganoderma bacterium obtained by step (4) is inoculated;
(6) cultural hypha: culturing room will be put into by the postvaccinal bacterium rod of step (5), and the temperature in this culturing room is controlled to be 24
oc, humid control is 85%, cultivates and obtains Ganoderma lucidum mycelium in 31 days; (7) sesame management is gone out: the Ganoderma lucidum mycelium plantation that will be obtained by step (6), in booth, keeps canopy temperature to be 20 in the process of growth of Ganoderma lucidum mycelium
oc, and be 60% by the humid control in this booth;
(8) timely collecting.
Embodiment two:
A kind of glossy ganoderma breeding method, comprises the steps:
(1) medium preparation: medium solid portion adopts the raw material of following percentage by weight to obtain: corncob 60wt%, soybean stalk 15wt%, glossy ganoderma slag 18wt%, soya bean soya-bean cake 3wt%, sucrose 2wt% and land plaster 2wt%, and in this medium solid portion, add water mix acquisition medium, now, the humid control of this medium is controlled 5.7 at 54%, pH;
(2) medium pack and sterilizing: the medium prepared by step (1) is loaded edible mushroom bag immediately, and the edible mushroom bag after charging is put into atmospheric sterilizing stove and 96
osterilizing 23 hours are maintained during C;
(3) cultivate the former bacterium of glossy ganoderma: be inoculated in the edible mushroom bag obtained by step (2) by female for glossy ganoderma bacterium, be then placed in aseptic space by postvaccinal edible mushroom bag, make the female bacterium of glossy ganoderma 25
ocarry out growth and breeding at the temperature of C, cultivate burmannaline camphorata mycelium in 28 days to edible mushroom bag, namely obtain the former bacterium of glossy ganoderma;
(4) cultivate cultivation of glossy ganoderma bacterium: be inoculated into by the former bacterium of glossy ganoderma obtained by step (3) in the edible mushroom bag obtained by step (2), then the edible mushroom bag of the former bacterium of inoculation glossy ganoderma is placed in aseptic space, makes the former bacterium of glossy ganoderma 25
ocarry out growth and breeding at the temperature of C, cultivate burmannaline camphorata mycelium in 20 days to edible mushroom bag, namely obtain cultivation of glossy ganoderma bacterium;
(5) bacterium rod inoculation: adopt Open-Architecture Controller mode, in the inoculation canopy adopting lime disinfection sterilized, bacterium rod is beaten inoculation hole successively, the distance between adjacent two inoculation holes is 11cm, and the hole depth in inoculation hole is 2.5cm, the diameter control in inoculation hole is 1.2cm; Then in inoculation hole, the cultivation of glossy ganoderma bacterium obtained by step (4) is inoculated;
(6) cultural hypha: culturing room will be put into by the postvaccinal bacterium rod of step (5), and the temperature in this culturing room is controlled to be 25
oc, humid control is 70%, cultivates and obtains Ganoderma lucidum mycelium in 30 days; (7) sesame management is gone out: the Ganoderma lucidum mycelium plantation that will be obtained by step (6), in booth, keeps canopy temperature to be 22 in the process of growth of Ganoderma lucidum mycelium
oc, and be 70% by the humid control in this booth;
(8) timely collecting.
Embodiment three:
A kind of glossy ganoderma breeding method, comprises the steps:
(1) medium preparation: medium solid portion adopts the raw material of following percentage by weight to obtain: corncob 63wt%, soybean stalk 5wt%, glossy ganoderma slag 25wt%, soya bean soya-bean cake 1wt%, sucrose 3wt% and land plaster 3wt%, and in this medium solid portion, add water mix acquisition medium, now, the humid control of this medium is controlled 5.5 at 58%, pH;
(2) medium pack and sterilizing: the medium prepared by step (1) is loaded edible mushroom bag immediately, and the edible mushroom bag after charging is put into atmospheric sterilizing stove and 98
osterilizing 22 hours are maintained during C;
(3) cultivate the former bacterium of glossy ganoderma: be inoculated in the edible mushroom bag obtained by step (2) by female for glossy ganoderma bacterium, be then placed in aseptic space by postvaccinal edible mushroom bag, make the female bacterium of glossy ganoderma 28
ocarry out growth and breeding at the temperature of C, cultivate burmannaline camphorata mycelium in 20 days to edible mushroom bag, namely obtain the former bacterium of glossy ganoderma;
(4) cultivate cultivation of glossy ganoderma bacterium: be inoculated into by the former bacterium of glossy ganoderma obtained by step (3) in the edible mushroom bag obtained by step (2), then the edible mushroom bag of the former bacterium of inoculation glossy ganoderma is placed in aseptic space, makes the former bacterium of glossy ganoderma 28
ocarry out growth and breeding at the temperature of C, cultivate burmannaline camphorata mycelium in 25 days to edible mushroom bag, namely obtain cultivation of glossy ganoderma bacterium;
(5) bacterium rod inoculation: adopt Open-Architecture Controller mode, in the inoculation canopy adopting lime disinfection sterilized, bacterium rod is beaten inoculation hole successively, the distance between adjacent two inoculation holes is 12cm, and the hole depth in inoculation hole is 3cm, the diameter control in inoculation hole is 1.5cm; Then in inoculation hole, the cultivation of glossy ganoderma bacterium obtained by step (4) is inoculated;
(6) cultural hypha: culturing room will be put into by the postvaccinal bacterium rod of step (5), and the temperature in this culturing room is controlled to be 26
oc, humid control is 60%, cultivates and obtains Ganoderma lucidum mycelium in 33 days; (7) sesame management is gone out: the Ganoderma lucidum mycelium plantation that will be obtained by step (6), in booth, keeps canopy temperature to be 25 in the process of growth of Ganoderma lucidum mycelium
oc, and be 80% by the humid control in this booth;
(8) timely collecting.
Embodiment four: a kind of glossy ganoderma breeding method, comprises the steps:
(1) medium preparation: medium solid portion adopts the raw material of following percentage by weight to obtain: corncob 75wt%, soybean stalk 5wt%, glossy ganoderma slag 10wt%, soya bean soya-bean cake 5wt%, sucrose 3wt% and land plaster 2wt%, and in this medium solid portion, add water mix acquisition medium, now, the humid control of this medium is controlled 5.0 at 60%, pH;
(2) medium pack and sterilizing: the medium prepared by step (1) is loaded edible mushroom bag immediately, and the edible mushroom bag after charging is put into atmospheric sterilizing stove and 100
osterilizing 20 hours are maintained during C;
(3) cultivate the former bacterium of glossy ganoderma: be inoculated in the edible mushroom bag obtained by step (2) by female for glossy ganoderma bacterium, be then placed in aseptic space by postvaccinal edible mushroom bag, make the female bacterium of glossy ganoderma 30
ocarry out growth and breeding at the temperature of C, cultivate burmannaline camphorata mycelium in 30 days to edible mushroom bag, namely obtain the former bacterium of glossy ganoderma;
(4) cultivate cultivation of glossy ganoderma bacterium: be inoculated into by the former bacterium of glossy ganoderma obtained by step (3) in the edible mushroom bag obtained by step (2), then the edible mushroom bag of the former bacterium of inoculation glossy ganoderma is placed in aseptic space, makes the former bacterium of glossy ganoderma 30
ocarry out growth and breeding at the temperature of C, cultivate burmannaline camphorata mycelium in 27 days to edible mushroom bag, namely obtain cultivation of glossy ganoderma bacterium;
(5) bacterium rod inoculation: adopt Open-Architecture Controller mode, in the inoculation canopy adopting lime disinfection sterilized, bacterium rod is beaten inoculation hole successively, the distance between adjacent two inoculation holes is 11cm, and the hole depth in inoculation hole is 2.8cm, the diameter control in inoculation hole is 1.3cm; Then in inoculation hole, the cultivation of glossy ganoderma bacterium obtained by step (4) is inoculated;
(6) cultural hypha: culturing room will be put into by the postvaccinal bacterium rod of step (5), and the temperature in this culturing room is controlled to be 28
oc, humid control is 50%, cultivates and obtains Ganoderma lucidum mycelium in 35 days; (7) sesame management is gone out: the Ganoderma lucidum mycelium plantation that will be obtained by step (6), in booth, keeps canopy temperature to be 28 in the process of growth of Ganoderma lucidum mycelium
oc, and be 95% by the humid control in this booth;
(8) timely collecting.
Should be understood that, for those of ordinary skills, can be improved according to the above description or convert, and all these improve and convert the protection domain that all should belong to claims of the present invention.
Claims (4)
1. a glossy ganoderma breeding method, is characterized in that, comprises the steps:
(1) medium preparation: described medium solid portion adopts the raw material of following percentage by weight to obtain: corncob 55-75wt%, soybean stalk 5-20wt%, glossy ganoderma slag 10-25wt%, soya bean soya-bean cake 1-5wt%, sucrose 1-3wt% and land plaster 1-3wt%, and in described medium solid portion, add water mix acquisition medium;
(2) medium pack and sterilizing: the medium prepared by described step (1) is loaded edible mushroom bag immediately, and the edible mushroom bag after charging is put into atmospheric sterilizing stove and carried out sterilizing;
(3) cultivate the former bacterium of glossy ganoderma: be inoculated in the edible mushroom bag obtained by described step (2) by female for glossy ganoderma bacterium, be then placed in aseptic space by postvaccinal edible mushroom bag, make the female bacterium of described glossy ganoderma at 20-30
ocarry out growth and breeding at the temperature of C, cultivate burmannaline camphorata mycelium in 20-30 days to edible mushroom bag, namely obtain the former bacterium of glossy ganoderma;
(4) cultivate cultivation of glossy ganoderma bacterium: be inoculated into by the former bacterium of glossy ganoderma obtained by described step (3) in the edible mushroom bag obtained by described step (2), then the edible mushroom bag of the former bacterium of inoculation glossy ganoderma is placed in aseptic space, makes the former bacterium of described glossy ganoderma at 20-30
ocarry out growth and breeding at the temperature of C, cultivate burmannaline camphorata mycelium in 20-30 days to edible mushroom bag, namely obtain cultivation of glossy ganoderma bacterium;
(5) bacterium rod inoculation: adopt Open-Architecture Controller mode, in the inoculation canopy that disinfection is crossed, bacterium rod is beaten inoculation hole successively, in described inoculation hole, then inoculates the cultivation of glossy ganoderma bacterium obtained by described step (4);
(6) cultural hypha: culturing room will be put into by the postvaccinal bacterium rod of described step (5), and the temperature in described culturing room is controlled as 24-28
oc, humid control is 50-85%, cultivates and obtains Ganoderma lucidum mycelium in 30-35 days; (7) sesame management is gone out: the Ganoderma lucidum mycelium plantation that will be obtained by described step (6), in booth, keeps described canopy temperature to be 20-28 in the process of growth of described Ganoderma lucidum mycelium
oc, and be 60-95% by the humid control in described booth;
(8) timely collecting;
In described step (2), the edible mushroom bag after charging is put into atmospheric sterilizing stove and 96
oc-98
osterilizing 22-23 hour is maintained during C;
In above-mentioned steps (5), the distance between upper adjacent two the inoculation holes of described bacterium rod is 10-12cm.
2. glossy ganoderma breeding method according to claim 1, is characterized in that, in described step (1), is controlled at 5.0-6.0 by the humid control of described medium at 50-60%, pH.
3. glossy ganoderma breeding method according to claim 1, is characterized in that, the hole depth in described inoculation hole is 2-3cm, and the diameter in described inoculation hole is 1-1.5cm.
4. glossy ganoderma breeding method according to claim 1, is characterized in that, in described step (5), adopts lime to carry out disinfection sterilization to described inoculation canopy.
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Cited By (1)
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| CN107295900A (en) * | 2017-08-15 | 2017-10-27 | 贵州天圣绿野生物科技有限公司 | A kind of breeding method of ganoderma lucidum |
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| CN101273693A (en) * | 2008-04-09 | 2008-10-01 | 河源市天仙湖农业发展有限公司 | Method for cultivating Ganoderma Sinense |
| CN101695258A (en) * | 2009-11-13 | 2010-04-21 | 福建岁昌生态农业开发有限公司 | Process for culturing lucid ganoderma |
| CN103125273B (en) * | 2013-03-14 | 2014-02-12 | 安徽省康美来大别山生物科技有限公司 | Ganoderma lucidum cultivation method |
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