CN105309246A - Root inoculation method for tobacco ingrowth biocontrol bacteria and tobacco disease and pest control method - Google Patents
Root inoculation method for tobacco ingrowth biocontrol bacteria and tobacco disease and pest control method Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protection of plants
Landscapes
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
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- Life Sciences & Earth Sciences (AREA)
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Abstract
The invention discloses a root inoculation method for tobacco ingrowth biocontrol bacteria and a tobacco disease and pest control method. The root inoculation method comprises the steps that in the process of transplanting tobacco seedlings, roots of the tobacco seedlings are dipped into a tobacco ingrowth biocontrol bacterial solution to make the tobacco ingrowth biocontrol bacteria colonized into a tobacco plant through broken root wounds in the roots. According to the root inoculation method, the tobacco ingrowth biocontrol bacteria are made to enter the root system of tobaccos through the broken root wounds in the tobacco seedling broken roots which are inevitably caused during transplantation of the tobacco seedlings and then are colonized into the tobacco plant; the success rate of inoculation is high, the survival rate of the ingrowth biocontrol bacteria colonized into the plant is high, and then the effect of remarkably preventing and controlling diseases and pests is achieved; since extra mechanical wounds are not caused to the tobacco plant, operation is simple, time and labor are saved, and the method is suitable for large-scale application; inoculation to the broken root wounds can be conducted at the same time as transplantation of the tobacco seedlings, so other pathogenic bacteria are prevented from entering the tobacco plant through the broken root wounds, infection of diseases and pests is prevented, and the method has application value in a wide range in practical production.
Description
Technical field
The invention belongs to tobacco diseases Prevention Technique field, be specifically related to a root inoculation method growing tobacco interior raw biocontrol microorganisms, also relate to a kind of prevention and controls of tobacco diseases simultaneously.
Background technology
Endophyte of plant referred in the certain phase of its history of life or whole stages, moved in the various tissue of health plant and the space between cells of organ or intracellular microorganism, comprised plant endogenesis epiphyte, endogenetic bacteria and endogeny rayungus.Endophyte of plant is almost present in all plants studied, and distribution is wide, and kind is many.Endophyte produces rich and varied secondary metabolite, has multiple biologically active, has important application potential in agricultural and pharmaceutical sector.Endophyte and its host plant common evolutionary, live in plant corpus, plant be endophyte provide one stable and have the environment of protectiveness and sufficient source of nutrition, these characteristics make it likely be studied as potential bio-control factors and utilize.
The endophyte with biological control effect is called interior raw biocontrol microorganisms.Carry out the principle of biological control with endogenetic bacteria, some antibiotic mainly utilizing endogenetic bacteria to produce in plant corpus are to the antagonism of pathogen, and some endogenetic bacteria can produce sterilization protein, also can play the effect killing pathogen; Some endogenetic bacteria can produce chitinase, can cracking fungal pathogen bacterium cell wall thus play Biocontrol Effect.In addition, endogenetic bacteria grows the ecological site being actually in plant corpus and occupying in plant corpus surely, due to the restriction of site and living space, prevents the invasion of pathogen and surely grows.Endogenetic bacteria can also form the Antagonistic Relationship of nutrient competition with pathogen, and pathogen is withered away because can not get normal nutrition supply.
Tobacco is leaf with economic crops, special requirement is had to the outward appearance of tobacco and inherent quality, residue of pesticide, pathogen pesticide resistance and the impact on cigarette health limit the extensive use of agricultural chemicals, therefore be necessary the Optimal Control approach inquiring into tobacco diseases, find new prophylactico-therapeutic measures to overcome agricultural chemical residue in tobacco and pathogen resistance problems.Tobacco endophyte kind is more, is valuable microbial resources storehouse, in Biological Control of Tobacco Diseases, the exploitation of tobacco growth promoting bacteria agent and reduction tobacco harmful substance etc., have huge application potential.Inhibiting endophyte can be had to pathogen by artificial screening, obtain the interior raw biocontrol microorganisms with diseases prevention activity.By Inoculation Method, the endophyte with diseases prevention activity is inoculated in tobacco plant, plays the prophylaxis effect of endophyte.
At present, it is fill with root inoculation method that the method for conventional artificial infection endophyte mainly contains following three kinds: one, with endophyte suspension pouring plant rhizosphere, endophyte can be made to enter in plant roots and stems; Two is seed soaking inoculation methods, prior to seeding the seed of plant is soaked certain hour in containing endophyte suspension, growing the endophyte be labeled can be detected in plant corpus; Three is smear or spray blade inoculation method, is smeared by endophyte liquid or is sprayed to plant leaf blade, also can by endophyte inoculation in various plants body.Said method is all the method for carrying out outside inoculation under the prerequisite not damaging plant, and success ratio of inoculation is low, and it is low that interior raw biocontrol microorganisms determining in plant body grows survival rate, and protection effect is poor, is not suitable for extensive use.
Summary of the invention
The object of this invention is to provide a root inoculation method growing tobacco interior raw biocontrol microorganisms, success ratio of inoculation is high, and interior raw biocontrol microorganisms determining in plant body grows that survival rate is high, and protection effect is good, is applicable to large-scale application.
Second object of the present invention is to provide a kind of prevention and controls of tobacco diseases.
In order to realize above object, the technical solution adopted in the present invention is:
The one root inoculation method growing tobacco interior raw biocontrol microorganisms, when being included in cigarette transplantation of seedlings, carries out dipping in root in raw biocontrol microorganisms bacterium liquid in tobacco by the root of cigarette seedling, to make in tobacco raw biocontrol microorganisms surely be grown in tobacco plant by root wound of being completely cured.
Cigarette transplantation of seedlings to complete the cigarette transplantation of seedlings of nursery to large Tanaka, and need cigarette seedling to shift out from original nursery or seedling-cultivating tray, this process inevitably causes cigarette seedling to be completely cured.Of the present invention inoculation method, after cigarette seedling is shifted out from original nursery or seedling-cultivating tray, the root of cigarette seedling is carried out dipping in root in raw biocontrol microorganisms bacterium liquid in tobacco, to make in tobacco raw biocontrol microorganisms surely be grown in tobacco plant by root wound of being completely cured, then by cigarette transplantation of seedlings to land for growing field crops.In this process, in tobacco, raw biocontrol microorganisms is entered by tobacco root and surely grows in tobacco plant, thus reaches the effect of control tobacco diseases.
The described time of dipping in root is 2 ~ 5s.
In tobacco of the present invention raw biocontrol microorganisms root inoculation method in, in the tobacco be suitable for raw biocontrol microorganisms be not limited to above-mentioned listed by, be all available for biocontrol microorganisms raw in the tobacco of biological tobacco control in prior art; Preferably, the preferred bacillus category biocontrol microorganisms of raw biocontrol microorganisms in described tobacco.
In described tobacco, raw biocontrol microorganisms is Endophytic Bacteria of Tobacco.Preferably, in contained in raw biocontrol microorganisms bacterium liquid in described tobacco tobacco, raw biocontrol microorganisms is bacillus amyloliquefaciens or tobacco lysine bacillus.Described bacillus amyloliquefaciens is preferably the double anti-root-knot nematode of patent document CN102925393B record and the tobacco endophyte-bacillus amyloliquefaciens BacillusamyloliquefaciensZY-9-13 bacterial strain of Phytophthora nicotianae; Described tobacco lysine bacillus is preferably tobacco endophyte bacterial strain-tobacco lysine bacillus (Lysinibacillustobaccocum) the Kpa bacterial strain of the anti-tobacco bacterial wilt that patent document CN102851245B records.In corresponding tobacco, the preparation method of raw biocontrol microorganisms bacterium liquid can refer to the record of patent document CN102925393B, CN102851245B.
In described tobacco in raw biocontrol microorganisms bacterium liquid, in tobacco, the viable count of raw biocontrol microorganisms is not less than 1 × 10
9cFU/mL.
The root inoculation method of raw biocontrol microorganisms in tobacco of the present invention, after cigarette seedling is shifted out from original nursery or seedling-cultivating tray, the root of cigarette seedling is carried out dipping in root in raw biocontrol microorganisms bacterium liquid in tobacco, raw biocontrol microorganisms is made in tobacco surely to be grown in tobacco plant by root wound of being completely cured, then by cigarette transplantation of seedlings to land for growing field crops; The method utilizes cigarette transplantation of seedlings inevitably to cause cigarette seedling to be completely cured, and raw biocontrol microorganisms enters tobacco root by root wound of being completely cured in tobacco, thus enters and surely grow in tobacco plant; Success ratio of inoculation is high, and it is high that interior raw biocontrol microorganisms determining in plant body grows survival rate, thus reach the effect of significant controlling disease; Do not need to cause extra Mechanical wound to tobacco plant, simple to operate, time saving and energy saving, be applicable to large-scale application; Wound inoculation of being completely cured can be carried out while cigarette transplantation of seedlings, avoiding other pathogens enters in tobacco plant by wound of being completely cured, thus avoid the dip-dye of wound by miscellaneous bacterias such as virus, bacteriums of being completely cured, prevent the infection of disease, be with a wide range of applications in actual production.
A prevention and controls for tobacco diseases, comprises the following steps:
1) after tobacco seedling leaf shearing, biocontrol microorganisms bacterium liquid raw in tobacco is sprayed or smears to tobacco seedling leaf shearing wound, raw biocontrol microorganisms in tobacco is grown in tobacco plant surely by leaf-cutting wound;
2) when cigarette transplantation of seedlings, the root of cigarette seedling is carried out dipping in root in raw biocontrol microorganisms bacterium liquid in tobacco, to make in tobacco raw biocontrol microorganisms surely be grown in tobacco plant by root wound of being completely cured.
Tobacco seedling leaf shearing is that tobacco seedling is suitably wiped out blade before transplant planting, is link necessary in modern tobacco plantation.The effect of leaf-cutting is mainly: cultivate more flourishing root system; Obtain more sturdy stem; Make uniform growth between seedling individuality; Control early blossoming; Seedling is made more to adapt to field planting etc.Utilize the correlation principle of cigarette seedling overground part and underground part, produced by leaf-cutting regulation and control cigarette seedling, avoid the formation of high pin seedling, increase stem thick, promote root system development, improve resistance.
In the prevention and controls of tobacco diseases of the present invention, cigarette transplantation of seedlings is after being shifted out from original nursery or seedling-cultivating tray by cigarette seedling, the root of cigarette seedling is carried out dipping in root in raw biocontrol microorganisms bacterium liquid in tobacco, raw biocontrol microorganisms is made in tobacco surely to be grown in tobacco plant by root wound of being completely cured, then by cigarette transplantation of seedlings to land for growing field crops.In this process, in tobacco, raw biocontrol microorganisms is entered by tobacco root and surely grows in tobacco plant, thus reaches the effect of control tobacco diseases.
Step 1) in, after tobacco seedling leaf shearing in 2h, biocontrol microorganisms bacterium liquid raw in tobacco is sprayed or smears to tobacco seedling leaf shearing wound.In said method, preferably immediately biocontrol microorganisms bacterium liquid raw in tobacco sprayed or smears to tobacco leaf-cutting wound after tobacco leaf-cutting, avoiding leaf-cutting wound by the dip-dye of the miscellaneous bacterias such as virus, bacterium; As interval time is long, other pathogens enter in tobacco plant by leaf-cutting wound, also manually will sterilize to wound, not only waste time and energy during inoculation, and agricultural chemicals sterilization can cause potential safety hazard to tobacco, environment and the mankind, residue of pesticide bring harmful effect can to cigarette health.
Tobacco seedling leaf shearing more than once, last tobacco seedling leaf shearing before cigarette transplantation of seedlings 4 ~ 6 days.The operation of tobacco leaf-cutting can adopt the conventional leaf-cutting technology in this area to carry out according to cigarette seedling growing way appearance; When tobacco seedling growth to five leaves wholeheartedly, stem height at least 5cm, completely when envelope dish start first time leaf-cutting; Within 5 ~ 7 days afterwards, cut second time, the rest may be inferred, and last leaf-cutting should before transplanting 4 ~ 6 days; Weather, fine day morning, and must carry out after dew is dry.
Step 2) in, described in dip in root time be 2 ~ 5s.
In described tobacco, raw biocontrol microorganisms is Endophytic Bacteria of Tobacco.Preferably, in contained in raw biocontrol microorganisms bacterium liquid in described tobacco tobacco, raw biocontrol microorganisms is bacillus amyloliquefaciens or tobacco lysine bacillus.
In described tobacco in raw biocontrol microorganisms bacterium liquid, in tobacco, the viable count of raw biocontrol microorganisms is not less than 1 × 10
9cFU/mL.
The prevention and controls of tobacco diseases of the present invention, original tobacco planting must leaf-cutting and cigarette transplantation of seedlings unavoidably cause cigarette seedling to be completely cured basis on, after tobacco leaf-cutting, sprayed or smear to tobacco leaf-cutting wound by biocontrol microorganisms bacterium liquid raw in tobacco, in tobacco, raw biocontrol microorganisms is grown in tobacco plant surely easily via leaf-cutting wound; During cigarette transplantation of seedlings, after cigarette seedling is shifted out from original nursery or seedling-cultivating tray, the root of cigarette seedling is carried out dipping in root in raw biocontrol microorganisms bacterium liquid in tobacco, to make in tobacco raw biocontrol microorganisms surely be grown in tobacco plant by root wound of being completely cured, then by cigarette transplantation of seedlings to land for growing field crops.This prevention and controls is worked along both lines, and the success ratio of inoculation of interior raw biocontrol microorganisms is high, and it is high that interior raw biocontrol microorganisms determining in plant body grows survival rate, thus reach the effect of significant controlling disease; Do not need to cause extra Mechanical wound to tobacco plant, simple to operate, time saving and energy saving, be applicable to large-scale application; The inoculation of leaf-cutting wound can be carried out while leaf-cutting, while cigarette transplantation of seedlings, carry out wound inoculation of being completely cured, avoiding other pathogens enters in tobacco plant by leaf-cutting wound and wound of being completely cured, thus avoid the dip-dye by miscellaneous bacterias such as virus, bacteriums of leaf-cutting wound and wound of being completely cured, prevent the infection of disease, be with a wide range of applications in actual production; Tobacco plant after this prevention and controls process grows vigorous large Tanaka, and the incidence of disease is low, and the quality of tobacco of production is high; The potential safety hazard that the use that simultaneously also avoid chemical pesticide brings to tobacco, environment and human body, has good economic benefit and environmental benefit.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated.
Embodiment 1
The root inoculation method of raw biocontrol microorganisms in the tobacco of the present embodiment, when cigarette transplantation of seedlings, after cigarette seedling is shifted out from original nursery or seedling-cultivating tray, the root of cigarette seedling is carried out dipping in root in raw biocontrol microorganisms bacterium liquid in tobacco, raw biocontrol microorganisms is made in tobacco surely to be grown in tobacco plant by root wound of being completely cured, then by cigarette transplantation of seedlings to land for growing field crops.
In the tobacco that in described tobacco, raw biocontrol microorganisms bacterium liquid contains, raw biocontrol microorganisms is tobacco endophyte bacterial strain-tobacco lysine bacillus (Lysinibacillustobaccocum) the Kpa bacterial strain of the anti-tobacco bacterial wilt that patent document CN102851245B records.
The preparation method of raw biocontrol microorganisms bacterium liquid in described tobacco: according to microbial inoculant method, (every 1000ml medium is containing maltose 10g bacterial strain Kpa (tobacco lysine bacillus Lysinibacillustobaccocum) to be inoculated into liquid nutrient medium, beef extract 1g, MgSO
47H
2o0.5g, KH
2pO
40.5g, Na
2hPO
40.65g, KCl0.5g, pH7.0-7.2) in; Be 32 DEG C in temperature, mixing speed is under 200rpm condition, and fermentation time 72 hours, to obtain final product.
In described tobacco in raw biocontrol microorganisms bacterium liquid, the viable count of tobacco lysine bacillus Kpa bacterial strain is 1.5 × 10
9cFU/mL; The time of dipping in root is 3s.
After tobacco seedling leaching root 15 days, adopt the endophyte (tobacco lysine bacillus Kpa bacterial strain) of microorganism conventional separation methods to seedling to be separated, calculating K pa bacterial strain determines at tobacco root the amount of growing.Tobacco seedling is transplanted latter 60 days, and statistics Kpa bacterial strain fermentation liquor is to the preventive effect of tobacco bacterial wilt.Contrast is streptomycin sulphate.
Result shows, tobacco seedling leaching root (Kpa bacterial strain fermentation liquor) is after 15 days, and having surely to grow in the middle part of tobacco rhizosphere soil, the tip of a root and root has Kpa bacterial strain (see table 1).After seedling field-transplanting 60 days, Kpa bacterial strain fermentation liquor was 68% to the preventive effect of tobacco bacterial wilt, a little more than streptomycin sulphate (table 1).
The rhizosphere colonization situation of table 1.Kpa bacterial strain and to tobacco bacterial wilt preventive effect
(after data, different lowercase alphabet is shown in 0.05 level difference significantly, and different capitalization represents remarkable at 0.01 level difference; "-" represents and does not detect biocontrol microorganisms.)
Embodiment 2
The root inoculation method of raw biocontrol microorganisms in the tobacco of the present embodiment, when cigarette transplantation of seedlings, after cigarette seedling is shifted out from original nursery or seedling-cultivating tray, the root of cigarette seedling is carried out dipping in root in raw biocontrol microorganisms bacterium liquid in tobacco, raw biocontrol microorganisms is made in tobacco surely to be grown in tobacco plant by root wound of being completely cured, then by cigarette transplantation of seedlings to land for growing field crops.
In the tobacco that in described tobacco, raw biocontrol microorganisms bacterium liquid contains, raw biocontrol microorganisms is the double anti-root-knot nematode of patent document CN102925393B record and the tobacco endophyte-bacillus amyloliquefaciens BacillusamyloliquefaciensZY-9-13 bacterial strain of Phytophthora nicotianae.
The preparation method of raw biocontrol microorganisms bacterium liquid in described tobacco: according to microbial inoculant method, bacterial strain ZY-9-13 is inoculated into KMB medium (glucose 30.0g, peptone 20.0g, MgSO
47H
2o1.5g, KH
2pO
41.5g, distilled water 1000ml, pH value 7.0), inoculum concentration is 3%, cultivation temperature 36 DEG C, incubation time 42h.With this CMC model ZY-9-13 bacterial strain, during 42h, cell concentration is 1.3 × 10
9cfu/ml.
In described tobacco in raw biocontrol microorganisms bacterium liquid, the viable count of bacillus amyloliquefaciens ZY-9-13 bacterial strain is 1.2 × 10
9cFU/mL; The time of dipping in root is 3s.
After tobacco seedling leaching root 15 days, adopt the endophyte (bacillus amyloliquefaciens ZY-9-13 bacterial strain) of microorganism conventional separation methods to seedling to be separated, calculate ZY-9-13 bacterial strain and determine at tobacco root the amount of growing.Tobacco seedling is transplanted latter 60 days, and statistics ZY-9-13 bacterial strain fermentation liquor is to the preventive effect of black shank.Contrast is metalaxyl (58%).
Result shows, tobacco seedling leaching root (ZY-9-13 bacterial strain fermentation liquor) is after 15 days, and tobacco rhizosphere is native, the tip of a root has surely to grow ZY-9-13 bacterial strain (see table 2).After seedling field-transplanting 60 days, ZY-9-13 bacterial strain fermentation liquor was 65% to the preventive effect of black shank, with chemical pesticide metalaxyl quite (table 2).
The rhizosphere colonization situation of table 2.ZY-9-13 bacterial strain and to black shank preventive effect
(after data, different lowercase alphabet is shown in 0.05 level difference significantly, and different capitalization represents remarkable at 0.01 level difference; "-" represents and does not detect biocontrol microorganisms.)
Embodiment 3
The prevention and controls of the tobacco diseases of the present embodiment, comprises the following steps:
1) leaf-cutting is carried out to cigarette seedling, at every turn after tobacco seedling leaf shearing, biocontrol microorganisms bacterium liquid raw in tobacco is sprayed or smears to tobacco seedling leaf shearing wound, raw biocontrol microorganisms in tobacco is grown in tobacco plant surely by leaf-cutting wound;
When tobacco seedling growth to five leaves wholeheartedly, stem height at least 5cm time start first time leaf-cutting; Within 5 ~ 7 days afterwards, cut second time, the rest may be inferred; Leaf-cutting, in fine day morning, is carried out after dew is dry;
2) tobacco seedling leaf shearing carried out cigarette transplantation of seedlings after 5 days the last time, after cigarette seedling being shifted out from original nursery or seedling-cultivating tray during transplanting, the root of cigarette seedling is carried out dipping in root in raw biocontrol microorganisms bacterium liquid in tobacco, raw biocontrol microorganisms is made in tobacco surely to be grown in tobacco plant by root wound of being completely cured, then by cigarette transplantation of seedlings to land for growing field crops.
In the tobacco that in described tobacco, raw biocontrol microorganisms bacterium liquid contains, raw biocontrol microorganisms is tobacco endophyte bacterial strain-tobacco lysine bacillus (Lysinibacillustobaccocum) the Kpa bacterial strain of the anti-tobacco bacterial wilt that patent document CN102851245B records.
In described tobacco, the preparation method of raw biocontrol microorganisms bacterium liquid is with embodiment 1.
In described tobacco in raw biocontrol microorganisms bacterium liquid, the viable count of tobacco lysine bacillus Kpa bacterial strain is 1.5 × 10
9cFU/mL; The time of dipping in root is 3s.
Embodiment 4
The prevention and controls of the tobacco diseases of the present embodiment, comprises the following steps:
1) leaf-cutting is carried out to cigarette seedling, at every turn after tobacco seedling leaf shearing, biocontrol microorganisms bacterium liquid raw in tobacco is sprayed or smears to tobacco seedling leaf shearing wound, raw biocontrol microorganisms in tobacco is grown in tobacco plant surely by leaf-cutting wound;
When tobacco seedling growth to five leaves wholeheartedly, stem height at least 5cm time start first time leaf-cutting; Within 5 ~ 7 days afterwards, cut second time, the rest may be inferred; Leaf-cutting, in fine day morning, is carried out after dew is dry;
2) tobacco seedling leaf shearing carried out cigarette transplantation of seedlings after 6 days the last time, after cigarette seedling being shifted out from original nursery or seedling-cultivating tray during transplanting, the root of cigarette seedling is carried out dipping in root in raw biocontrol microorganisms bacterium liquid in tobacco, raw biocontrol microorganisms is made in tobacco surely to be grown in tobacco plant by root wound of being completely cured, then by cigarette transplantation of seedlings to land for growing field crops.
In the tobacco that in described tobacco, raw biocontrol microorganisms bacterium liquid contains, raw biocontrol microorganisms is the double anti-root-knot nematode of patent document CN102925393B record and the tobacco endophyte-bacillus amyloliquefaciens BacillusamyloliquefaciensZY-9-13 bacterial strain of Phytophthora nicotianae.
In described tobacco, the preparation method of raw biocontrol microorganisms bacterium liquid is with embodiment 2.
In described tobacco in raw biocontrol microorganisms bacterium liquid, the viable count of bacillus amyloliquefaciens ZY-9-13 bacterial strain is 1.2 × 10
9cFU/mL; The time of dipping in root is 3s.
Claims (10)
1. a root inoculation method growing tobacco interior raw biocontrol microorganisms, is characterized in that: when being included in cigarette transplantation of seedlings, is carried out dipping in root by the root of cigarette seedling in tobacco in raw biocontrol microorganisms bacterium liquid, to make in tobacco raw biocontrol microorganisms surely be grown in tobacco plant by root wound of being completely cured.
2. the root inoculation method of raw biocontrol microorganisms in tobacco according to claim 1, is characterized in that: described in dip in root time be 2 ~ 5s.
3. the root inoculation method of the interior raw biocontrol microorganisms of tobacco according to claim 1 and 2, is characterized in that: in described tobacco, raw biocontrol microorganisms is Endophytic Bacteria of Tobacco.
4. the root inoculation method of the interior raw biocontrol microorganisms of tobacco according to claim 1 and 2, is characterized in that: in described tobacco in raw biocontrol microorganisms bacterium liquid, in tobacco, the viable count of life biocontrol microorganisms is not less than 1 × 10
9cFU/mL.
5. a prevention and controls for tobacco diseases, is characterized in that: comprise the following steps:
1) after tobacco seedling leaf shearing, biocontrol microorganisms bacterium liquid raw in tobacco is sprayed or smears to tobacco seedling leaf shearing wound, raw biocontrol microorganisms in tobacco is grown in tobacco plant surely by leaf-cutting wound;
2) when cigarette transplantation of seedlings, the root of cigarette seedling is carried out dipping in root in raw biocontrol microorganisms bacterium liquid in tobacco, to make in tobacco raw biocontrol microorganisms surely be grown in tobacco plant by root wound of being completely cured.
6. the prevention and controls of tobacco diseases according to claim 5, is characterized in that: step 1) in, after tobacco seedling leaf shearing in 2h, biocontrol microorganisms bacterium liquid raw in tobacco is sprayed or smears to tobacco seedling leaf shearing wound.
7. the prevention and controls of tobacco diseases according to claim 5, is characterized in that: tobacco seedling leaf shearing more than once, last tobacco seedling leaf shearing before cigarette transplantation of seedlings 4 ~ 6 days.
8. the prevention and controls of tobacco diseases according to claim 5, is characterized in that: step 2) in, described in dip in root time be 2 ~ 5s.
9. the prevention and controls of the tobacco diseases according to claim 5,6 or 8, is characterized in that: in described tobacco, raw biocontrol microorganisms is Endophytic Bacteria of Tobacco.
10. the prevention and controls of the tobacco diseases according to claim 5,6 or 8, is characterized in that: in described tobacco in raw biocontrol microorganisms bacterium liquid, in tobacco, the viable count of raw biocontrol microorganisms is not less than 1 × 10
9cFU/mL.
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Application publication date: 20160210 |