CN106234033A - Husky pestle mushroom artificial acclimation method - Google Patents
Husky pestle mushroom artificial acclimation method Download PDFInfo
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- CN106234033A CN106234033A CN201610731022.4A CN201610731022A CN106234033A CN 106234033 A CN106234033 A CN 106234033A CN 201610731022 A CN201610731022 A CN 201610731022A CN 106234033 A CN106234033 A CN 106234033A
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- pestle
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- husky
- spore
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Abstract
The invention discloses a kind of husky pestle mushroom artificial acclimation method, comprise the steps: 1) cultivate strain: gather wild husky pestle mushroom, and select individual excellent sporophore to carry out spore separation, obtain spore and the husky wild trophosome of pestle mushroom, select excellent spore standby；Carry out separate tissue between each position to the husky wild trophosome of pestle mushroom, and carry out organizing detoxification, purification, rejuvenation, obtain virus-free pure mycelium；2) cultivate original seed: a. select rule just, be of moderate size and the homophyletic that selects in multiple step 1) that germination is strong or the excellent spore of its strain of the same race；B. promote to merge between spore with biological preparation EDTA fusogen；C. " acclimatizing culture medium " and " mould leaching culture medium " is utilized to tame it to the adaptability of native country raw material and disease resistance；D. repeat step a to step c, filter out degrees of fusion closely, mycelial growth power is strong, resistance and sporophore do very well bacterial strain；3) cultigen is cultivated: formulate the Cultural plan of husky pestle mushroom.
Description
Technical field
The invention belongs to the technical field of cultivation of mushroom, be specifically related to a kind of husky pestle mushroom artificial acclimation method.
Background technology
Ordos City boolean make pottery last of the twelve Earthly Branches desert area Folium et Cacumen Artemisiae Halodendri under, grow a kind of wild mushroom, local villager is referred to as " husky
Wooden club ", annual spring and autumn, locality peasants and herdsmen search for food in a large number, and a person sponging on an aristocrat a long way is the most in an endless stream.Now is due to the market demand
Rapidly increasing, indiscriminate mining and serious waste are the most serious so that this precious and rare Resources of The Wild Edible Fungi frequency domain becomes extinct.
Summary of the invention
In view of this, it is an object of the invention to provide a kind of husky pestle mushroom artificial acclimation method, tame to realize
Technique effect.
For reaching above-mentioned purpose, the present invention provides following technical scheme:
A kind of husky pestle mushroom artificial acclimation method, comprises the steps:
1) strain is cultivated: gather wild husky pestle mushroom, and select individual excellent sporophore to carry out spore separation, obtain spore and sand
The wild trophosome of pestle mushroom, selects excellent spore standby；Separate tissue is carried out between each position to the husky wild trophosome of pestle mushroom,
And carry out organizing detoxification, purification, rejuvenation, obtain virus-free pure mycelium；
2) original seed is cultivated:
A. select rule just, be of moderate size and the homophyletic that selects in multiple described step 1) that germination is strong or the institute of its strain of the same race
State excellent spore；
B. promote to merge between spore with biological preparation EDTA fusogen；
C. " acclimatizing culture medium " and " mould leaching culture medium " is utilized to tame it to the adaptability of native country raw material and disease resistance；
D. repeat step a to step c, filter out degrees of fusion closely, mycelial growth power is strong, resistance and sporophore do very well
Bacterial strain；After spore is combined closely with mycelia formation plasmodesmata, spore mycelia variability is utilized to merge；
3) cultigen is cultivated: control the temperature of multiple raising chamber, humidity, moisture, ventilation, illuminance and nutritional condition respectively, right
Than the bacterium product growth conditions in each raising chamber, formulate the Cultural plan of husky pestle mushroom.
Further, the preparation method of described acclimatizing culture medium is as follows: according to Folium et Cacumen Artemisiae Halodendri powder 20-50 part；Corn stalk powder: 10-40
Part；Dry cattle manure: 30 parts；Dry sheep stool: the husky pestle mushroom planting material of weight ratio preparation of 10 parts, takes 300g sand pestle mushroom planting material and adds
2000ml water boil filtered after 50 minutes, then took 1000ml filtrate and join agar 20g, glucose 15g, starch 36g.
Further, the preparation method of described mould leaching culture medium is: the mould material of the husky pestle mushroom infected by band green mold bacterium claims 1kg also
Add the water of 2kg, take the leachate of 1kg, make leachate mass content and be respectively the culture medium of 5%, 10%, 15%, and by this cultivation
Base is tamed by mutagenicity.
Further, in described step 3), the Cultural plan of described husky pestle mushroom is: temperature: 22-25 DEG C；Humidity: 85%-95%；
Moisture: 55%；Illuminance, sporophore growth needs 60-120LX.
Further, in described step 3), the cultivation nutrient condition of described husky pestle mushroom is: Folium et Cacumen Artemisiae Halodendri 20 parts, corn stalk silk 40 parts,
Cattle manure 30 parts, dry sheep stool 10 parts and the mixing after fermentation 16 days of 2 parts of Gypsum Fibrosum, turning twice during fermentation, and to regulate PH be 7.5 trainings
Support.
The beneficial effects of the present invention is:
The husky pestle mushroom artificial acclimation method of the present invention, by understanding and analyzing husky pestle mushroom ecological environment, life habit, soil, gas
The requirement etc. waited, the special biological characteristics foundation making artificial domesticating cultivation, and gather wild sporophore, by laboratory
Separate spore and single mycelium, pure culture secondary mycelium, tame through ' adaptability culture medium ', it is achieved tame technology
Effect, the not only specification local farmers plantation to wild mushroom, reduce Planting risk, add income, and save
The strain of this wild resource and establish the planting system of wild mushroom resource, thus it is famous and precious and rare wild to protect this
Bacterium resource.
Detailed description of the invention
Below in conjunction with specific embodiment, the invention will be further described, so that those skilled in the art can be more preferable
Understand the present invention and can be practiced, but illustrated embodiment is not as a limitation of the invention.
A kind of husky pestle mushroom artificial acclimation method of the present embodiment, comprises the steps:
1) strain is cultivated: gather wild husky pestle mushroom, and select individual excellent sporophore to carry out spore separation, obtain spore and sand
The wild trophosome of pestle mushroom, selects excellent spore standby；Separate tissue is carried out between each position to the husky wild trophosome of pestle mushroom,
And carry out organizing detoxification, purification, rejuvenation, obtain virus-free pure mycelium；
2) original seed is cultivated:
A. select rule just, be of moderate size and the homophyletic that selects in multiple described step 1) that germination is strong or the institute of its strain of the same race
State excellent spore；
B. promote to merge between spore with biological preparation EDTA fusogen；
C. " acclimatizing culture medium " and " mould leaching culture medium " is utilized to tame it to the adaptability of native country raw material and disease resistance；
The preparation method of described acclimatizing culture medium is as follows: according to Folium et Cacumen Artemisiae Halodendri powder 20-50 part；Corn stalk powder: 10-40 part；Dry cattle manure: 30
Part；Dry sheep stool: the husky pestle mushroom planting material of weight ratio preparation of 10 parts, takes 300g sand pestle mushroom planting material and adds 2000ml water boil again
Filter after 50 minutes, then take 1000ml filtrate and join agar 20g, glucose 15g, starch 36g, the husky pestle mushroom planting material of the present embodiment
Proportioning as follows: 20 parts of Folium et Cacumen Artemisiae Halodendri powder；Corn stalk powder: 40 parts；Dry cattle manure: 30 parts；Dry sheep stool: 10 parts；Certainly, husky pestle mushroom planting material
Proportioning can also be: 50 parts of Folium et Cacumen Artemisiae Halodendri powder；Corn stalk powder: 10 parts；Dry cattle manure: 30 parts；Dry sheep stool: 10 parts；Or 30 parts of Folium et Cacumen Artemisiae Halodendri powder；
Corn stalk powder: 30 parts；Dry cattle manure: 30 parts；Dry sheep stool: 10 parts, all can meet use requirement, and its principle is suitable, is not repeated.
The preparation method of described mould leaching culture medium is: the mould material of the husky pestle mushroom infected by band green mold bacterium claims 1kg and adds 2kg's
Water, takes the leachate of 1kg, makes leachate mass content and is respectively the culture medium of 5%, 10%, 15%, and this culture medium is passed through
Mutagenicity is tamed；
D. repeat step a to step c, filter out degrees of fusion closely, mycelial growth power is strong, resistance and sporophore do very well
Bacterial strain；After spore is combined closely with mycelia formation plasmodesmata, spore mycelia variability is utilized to merge；
3) cultigen is cultivated: control the temperature of multiple raising chamber, humidity, moisture, ventilation, illuminance and nutritional condition respectively, right
Than the bacterium product growth conditions in each raising chamber, formulate the Cultural plan of husky pestle mushroom.The cultivation nutrient of the husky pestle mushroom of the present embodiment
Condition is: Folium et Cacumen Artemisiae Halodendri 20 parts, corn stalk silk 40 parts, cattle manure 30 parts, dry sheep stool 10 parts and 2 parts of Gypsum Fibrosum mix after fermentation 16 days, fermentation
Period turning twice, and to regulate PH be 7.5 cultivations.Temperature in raising chamber: 22-25 DEG C；Humidity: 85%-95%；Moisture: 55%；
Ventilate: bigger；Illuminance, sporophore growth needs 60-120LX, concrete, and the temperature in the raising chamber of the present embodiment is: 23
℃；Humidity: 90%；Moisture: 55%；Ventilate: bigger；Illuminance, sporophore growth needs 90LX.Certainly, the environment in raising chamber
Condition can also be: temperature is: 22 DEG C；Humidity: 95%；Moisture: 55%；Ventilate: bigger；Illuminance, sporophore growth needs
60LX；Or temperature is: 25 DEG C；Humidity: 85%；Moisture: 55%；Ventilate: bigger；Illuminance, sporophore growth needs 120LX.
The husky pestle mushroom artificial acclimation method of the present embodiment, by understanding and analyzing husky pestle mushroom ecological environment, life habit, soil
Earth, the requirement etc. of weather, the special biological characteristics foundation making artificial domesticating cultivation, and gather wild sporophore, by
Laboratory separation spore and single mycelium, pure culture secondary mycelium, tame through ' adaptability culture medium ', it is achieved artificial culture
Technique effect, the not only specification local farmers plantation to wild mushroom, reduce Planting risk, add income, and
Save the strain of this wild resource and establish the planting system of wild mushroom resource, thus it is famous and precious and rare to protect this
Wild mushroom resource.
Embodiment described above is only the preferred embodiment lifted by absolutely proving the present invention, the protection model of the present invention
Enclose and be not limited to this.The equivalent that those skilled in the art are made on the basis of the present invention substitutes or conversion, all in the present invention
Protection domain within.Protection scope of the present invention is as the criterion with claims.
Claims (5)
1. a husky pestle mushroom artificial acclimation method, it is characterised in that: comprise the steps:
1) strain is cultivated: gather wild husky pestle mushroom, and select individual excellent sporophore to carry out spore separation, obtain spore and sand
The wild trophosome of pestle mushroom, selects excellent spore standby；Separate tissue is carried out between each position to the husky wild trophosome of pestle mushroom,
And carry out organizing detoxification, purification, rejuvenation, obtain virus-free pure mycelium；
2) original seed is cultivated:
A. select rule just, be of moderate size and the homophyletic that selects in multiple described step 1) that germination is strong or the institute of its strain of the same race
State excellent spore；
B. promote to merge between spore with biological preparation EDTA fusogen；
C. " acclimatizing culture medium " and " mould leaching culture medium " is utilized to tame it to the adaptability of native country raw material and disease resistance；
D. repeat step a to step c, filter out degrees of fusion closely, mycelial growth power is strong, resistance and sporophore do very well
Bacterial strain；After spore is combined closely with mycelia formation plasmodesmata, spore mycelia variability is utilized to merge；
3) cultigen is cultivated: control the temperature of multiple raising chamber, humidity, moisture, ventilation, illuminance and nutritional condition respectively, right
Than the bacterium product growth conditions in each raising chamber, formulate the Cultural plan of husky pestle mushroom.
Husky pestle mushroom artificial acclimation method the most according to claim 1, it is characterised in that: the preparation side of described acclimatizing culture medium
Method is as follows: according to Folium et Cacumen Artemisiae Halodendri powder 20-50 part；Corn stalk powder: 10-40 part；Dry cattle manure: 30 parts；Dry sheep stool: the weight ratio of 10 parts
The husky pestle mushroom planting material of preparation, takes 300g sand pestle mushroom planting material and adds 2000ml water boil again and filters after 50 minutes, then takes 1000ml and filter
Liquid joins agar 20g, glucose 15g, starch 36g.
Husky pestle mushroom artificial acclimation method the most according to claim 1, it is characterised in that: the preparation side of described mould leaching culture medium
Method is: the mould material of the husky pestle mushroom infected by band green mold bacterium claims 1kg and adds the water of 2kg, takes the leachate of 1kg, makes leachate matter
Amount content is respectively the culture medium of 5%, 10%, 15%, and this culture medium is tamed by mutagenicity.
Husky pestle mushroom artificial acclimation method the most according to claim 1, it is characterised in that: in described step 3), described husky pestle
The Cultural plan of mushroom is: temperature: 22-25 DEG C；Humidity: 85%-95%；Moisture: 55%；Illuminance, sporophore growth needs 60-
120LX。
Husky pestle mushroom artificial acclimation method the most according to claim 1, it is characterised in that: in described step 3), described husky pestle
The cultivation nutrient condition of mushroom is: send out after Folium et Cacumen Artemisiae Halodendri 20 parts, corn stalk silk 40 parts, cattle manure 30 parts, dry sheep stool 10 parts and the mixing of 2 parts of Gypsum Fibrosum
Ferment 16 days, turning twice during fermentation, and to regulate PH be 7.5 cultivations.
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| CN201610731022.4A CN106234033A (en) | 2016-08-26 | 2016-08-26 | Husky pestle mushroom artificial acclimation method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107988134A (en) * | 2018-01-19 | 2018-05-04 | 中国农业大学 | A kind of strain domestication method for improving bacillus production gemma rate |
| CN110651665A (en) * | 2019-10-25 | 2020-01-07 | 葫芦岛农函大玄宇食用菌野驯繁育有限公司 | Lucid ganoderma culture medium, preparation method and method for culturing lucid ganoderma by using culture medium |
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| CN101637100A (en) * | 2009-09-01 | 2010-02-03 | 南阳天冠种业有限公司 | Artificial acclimation method of agrocybe prauox fayod |
| CN104694618A (en) * | 2015-03-13 | 2015-06-10 | 河南省农业科学院植物保护研究所 | Detection method for disease resistance of cotton verticillium wilt |
| CN105410035A (en) * | 2015-12-08 | 2016-03-23 | 济南舜昊生物科技有限公司 | Compounded preparation containing flusilazole |
| CN105494088A (en) * | 2015-12-21 | 2016-04-20 | 镇江盛弘景观植物有限公司 | Disease-resistant variety breeding method of wild ganoderma lucidum |
-
2016
- 2016-08-26 CN CN201610731022.4A patent/CN106234033A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1966668A (en) * | 2005-11-15 | 2007-05-23 | 叶兆增 | Method for solving the problem of bacteria strain aging |
| CN101637100A (en) * | 2009-09-01 | 2010-02-03 | 南阳天冠种业有限公司 | Artificial acclimation method of agrocybe prauox fayod |
| CN104694618A (en) * | 2015-03-13 | 2015-06-10 | 河南省农业科学院植物保护研究所 | Detection method for disease resistance of cotton verticillium wilt |
| CN105410035A (en) * | 2015-12-08 | 2016-03-23 | 济南舜昊生物科技有限公司 | Compounded preparation containing flusilazole |
| CN105494088A (en) * | 2015-12-21 | 2016-04-20 | 镇江盛弘景观植物有限公司 | Disease-resistant variety breeding method of wild ganoderma lucidum |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107988134A (en) * | 2018-01-19 | 2018-05-04 | 中国农业大学 | A kind of strain domestication method for improving bacillus production gemma rate |
| CN110651665A (en) * | 2019-10-25 | 2020-01-07 | 葫芦岛农函大玄宇食用菌野驯繁育有限公司 | Lucid ganoderma culture medium, preparation method and method for culturing lucid ganoderma by using culture medium |
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Application publication date: 20161221 |