CN104053772A - 乙内酰脲酶的突变体 - Google Patents

乙内酰脲酶的突变体 Download PDF

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Publication number
CN104053772A
CN104053772A CN201280067105.8A CN201280067105A CN104053772A CN 104053772 A CN104053772 A CN 104053772A CN 201280067105 A CN201280067105 A CN 201280067105A CN 104053772 A CN104053772 A CN 104053772A
Authority
CN
China
Prior art keywords
seq
amino acid
hydantoinase
hydantoin
acid sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201280067105.8A
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English (en)
Chinese (zh)
Inventor
S·奥斯瓦尔德
H·舒斯特
J·罗斯
A·卡劳
U·施瓦内贝格
R·马丁内斯
H·蒙德哈达
U·霍尔特
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Evonik Industries AG
Original Assignee
Evonik Industries AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evonik Industries AG filed Critical Evonik Industries AG
Priority to CN201811207287.XA priority Critical patent/CN109468304B/zh
Publication of CN104053772A publication Critical patent/CN104053772A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • C12P13/227—Tryptophan
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
    • C12P41/006—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by reactions involving C-N bonds, e.g. nitriles, amides, hydantoins, carbamates, lactames, transamination reactions, or keto group formation from racemic mixtures
    • C12P41/009—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by reactions involving C-N bonds, e.g. nitriles, amides, hydantoins, carbamates, lactames, transamination reactions, or keto group formation from racemic mixtures by reactions involving hydantoins or carbamoylamino compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/02—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amides (3.5.2)
    • C12Y305/02002—Dihydropyrimidinase (3.5.2.2), i.e. hydantoinase

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201280067105.8A 2011-11-16 2012-11-16 乙内酰脲酶的突变体 Pending CN104053772A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201811207287.XA CN109468304B (zh) 2011-11-16 2012-11-16 乙内酰脲酶的突变体

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP11189395.4 2011-11-16
EP11189395 2011-11-16
PCT/EP2012/072891 WO2013072486A1 (en) 2011-11-16 2012-11-16 Mutants of hydantoinase

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN201811207287.XA Division CN109468304B (zh) 2011-11-16 2012-11-16 乙内酰脲酶的突变体

Publications (1)

Publication Number Publication Date
CN104053772A true CN104053772A (zh) 2014-09-17

Family

ID=47189946

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201280067105.8A Pending CN104053772A (zh) 2011-11-16 2012-11-16 乙内酰脲酶的突变体
CN201811207287.XA Expired - Fee Related CN109468304B (zh) 2011-11-16 2012-11-16 乙内酰脲酶的突变体

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN201811207287.XA Expired - Fee Related CN109468304B (zh) 2011-11-16 2012-11-16 乙内酰脲酶的突变体

Country Status (12)

Country Link
US (2) US9957536B2 (enExample)
EP (1) EP2780451B1 (enExample)
JP (1) JP6279478B2 (enExample)
KR (1) KR102027201B1 (enExample)
CN (2) CN104053772A (enExample)
BR (1) BR112014011664A2 (enExample)
CA (1) CA2856127C (enExample)
DK (1) DK2780451T3 (enExample)
ES (1) ES2652387T3 (enExample)
IN (1) IN2014KN00866A (enExample)
PL (1) PL2780451T3 (enExample)
WO (1) WO2013072486A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112175929A (zh) * 2020-10-10 2021-01-05 山西大学 一种重组海因消旋酶工程菌及其构建方法和用途

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4105335A1 (en) 2021-06-16 2022-12-21 Evonik Operations GmbH Enzymatic method for the production of l-glufosinate p-alkyl esters
CN114686465B (zh) * 2021-11-21 2024-03-22 宁波酶赛生物工程有限公司 一种水解酶和一种(r)-(-)-3-(氨甲酰甲基)-5-甲基己酸的合成方法
WO2023174511A1 (en) 2022-03-14 2023-09-21 Evonik Operations Gmbh Enzymatic method for the production of l-glufosinate p-esters
WO2023222227A1 (en) 2022-05-19 2023-11-23 Evonik Operations Gmbh Enzymatic method for producing l-glufosinate
WO2023222226A1 (en) 2022-05-19 2023-11-23 Evonik Operations Gmbh Enzymatic method for producing l-glufosinate
WO2023232225A1 (en) 2022-05-31 2023-12-07 Evonik Operations Gmbh Enzymatic method for the diastereoselective production of l-glufosinate p-esters

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000058449A1 (en) * 1999-03-29 2000-10-05 California Institute Of Technology Hydantoinase variants with improved properties and their use for the production of amino acids
CN1458276A (zh) * 2002-05-13 2003-11-26 中国人民解放军军事医学科学院生物工程研究所 一种乙内酰脲酶系及其编码基因与生产菌

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5391189A (en) * 1976-12-30 1978-08-10 Kanegafuchi Chem Ind Co Ltd Preparation of d-n-carbamoyl-alpha-amino acids
JPS6225990A (ja) * 1986-07-19 1987-02-03 Kanegafuchi Chem Ind Co Ltd Ｄ−α−アミノ酸類の製造方法
DE4316928C2 (de) 1993-05-19 1995-03-16 Degussa Neue Mikroorganismen, deren Verwendung und Verfahren zur Herstellung von L-alpha-Aminosäuren
DE10114999A1 (de) 2001-03-26 2002-10-10 Degussa D-Carbamoylase aus Arthrobacter crystallopoietes DSM 20117
DE10155928A1 (de) 2001-11-15 2003-06-12 Degussa recA-negativer und rhaB-negativer Mikroorganismus
DE10251184A1 (de) * 2002-11-04 2004-05-13 Degussa Ag Mutanten zur Herstellung von D-Aminosäuren
JP2007189949A (ja) * 2006-01-19 2007-08-02 Kaneka Corp 新規ヒダントイナーゼ及び光学活性アミノ酸誘導体の製造方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000058449A1 (en) * 1999-03-29 2000-10-05 California Institute Of Technology Hydantoinase variants with improved properties and their use for the production of amino acids
CN1458276A (zh) * 2002-05-13 2003-11-26 中国人民解放军军事医学科学院生物工程研究所 一种乙内酰脲酶系及其编码基因与生产菌

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CHEON YH ET AL.: "Manipulation of the active site loops of D-hydantoinase,a(beta/alpha)8-barrel protein,for modulation of the substrate specificity.", 《BIOCHEMISTRY》 *
YOUNG-HOON CHEON ET AL.: "Manipulation of the active site loops of D-hydantoinase,a(β/α)8-barrel protein,for modulation of the substrate specificity", 《BIOCHEMISTRY》 *
ZHEN XU ET AL.: "Crystal structure of D-hydantoinase from Burkholderia pickettii at a resolution of 2.7 angstroms:insights into the molecular basis of enzyme thermostability", 《JOURNAL OF BACTERIOLOGY》 *
王婕等: "乙内酰脲酶及其在氨基酸手性合成中的应用", 《生物技术通讯》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112175929A (zh) * 2020-10-10 2021-01-05 山西大学 一种重组海因消旋酶工程菌及其构建方法和用途

Also Published As

Publication number Publication date
US9957536B2 (en) 2018-05-01
CN109468304B (zh) 2022-04-19
CA2856127A1 (en) 2013-05-23
US20150056667A1 (en) 2015-02-26
JP2014533497A (ja) 2014-12-15
CA2856127C (en) 2021-04-06
ES2652387T3 (es) 2018-02-01
CN109468304A (zh) 2019-03-15
JP6279478B2 (ja) 2018-02-14
EP2780451A1 (en) 2014-09-24
WO2013072486A1 (en) 2013-05-23
US10822627B2 (en) 2020-11-03
KR20140092835A (ko) 2014-07-24
DK2780451T3 (en) 2018-01-02
BR112014011664A2 (pt) 2017-05-09
KR102027201B1 (ko) 2019-10-01
US20180237814A1 (en) 2018-08-23
IN2014KN00866A (enExample) 2015-10-02
PL2780451T3 (pl) 2018-03-30
EP2780451B1 (en) 2017-09-20

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C06 Publication
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SE01 Entry into force of request for substantive examination
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TA01 Transfer of patent application right

Effective date of registration: 20160411

Address after: Dossenheim Gutenberg Street No. 2 69221

Applicant after: EVONIK INDUSTRIES CHEMICAL CO. LTD.

Address before: essen

Applicant before: Evonik Industries AG

RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20140917