CN104040350B - 用于检测多特异性结合物的游离结合搭档的方法 - Google Patents

用于检测多特异性结合物的游离结合搭档的方法 Download PDF

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Publication number
CN104040350B
CN104040350B CN201280063032.5A CN201280063032A CN104040350B CN 104040350 B CN104040350 B CN 104040350B CN 201280063032 A CN201280063032 A CN 201280063032A CN 104040350 B CN104040350 B CN 104040350B
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antibody
antigen
specificity
sample
idiotype
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CN104040350A (zh
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K-G·施图本拉赫
U·韦塞尔斯
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F Hoffmann La Roche AG
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
CN201280063032.5A 2011-12-19 2012-12-18 用于检测多特异性结合物的游离结合搭档的方法 Active CN104040350B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP11194350.2 2011-12-19
EP11194350 2011-12-19
PCT/EP2012/075991 WO2013092611A2 (en) 2011-12-19 2012-12-18 Method for the detection of free binding partner of a multispecific binder

Publications (2)

Publication Number Publication Date
CN104040350A CN104040350A (zh) 2014-09-10
CN104040350B true CN104040350B (zh) 2016-05-18

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Country Link
US (1) US9671394B2 (enExample)
EP (1) EP2795335B1 (enExample)
JP (1) JP6203747B2 (enExample)
KR (1) KR20140099277A (enExample)
CN (1) CN104040350B (enExample)
BR (1) BR112014011535A2 (enExample)
CA (1) CA2853917A1 (enExample)
MX (1) MX349003B (enExample)
RU (1) RU2633488C2 (enExample)
WO (1) WO2013092611A2 (enExample)

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US8956859B1 (en) 2010-08-13 2015-02-17 Aviex Technologies Llc Compositions and methods for determining successful immunization by one or more vaccines
BR112014017630A8 (pt) 2012-02-01 2017-07-11 Hoffmann La Roche Métodos para a determinação de presença e/ou da quantidade de um antígeno
JP6226976B2 (ja) 2012-07-13 2017-11-08 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 多重特異性結合物を検出するための方法
EP3066469B1 (en) * 2013-11-05 2017-10-11 Roche Diagnostics GmbH A method for determining the total amount and/or concentration of an analyte in the presence of a binding molecule as well as kits, compositions and uses relating thereto
WO2015143277A1 (en) * 2014-03-20 2015-09-24 Wellstat Diagnostics, Llc Antibodies and methods for the detection of cell death
ES2761427T3 (es) 2014-12-18 2020-05-19 Hoffmann La Roche Procedimientos para reducir interferencias
JP2020530554A (ja) * 2017-07-20 2020-10-22 シートムエックス セラピューティクス，インコーポレイテッド 活性化抗体の特性を定性的および／または定量的に分析する方法およびその使用
CN109358192B (zh) * 2018-02-08 2020-05-12 中国科学院上海药物研究所 一种去除抗药抗体检测样品中游离药物的装置和方法、该装置的制备方法及应用
EP3805757A4 (en) 2018-06-04 2022-03-23 Chugai Seiyaku Kabushiki Kaisha Method for detecting complex
SG11202110911RA (en) * 2019-05-13 2021-10-28 Regeneron Pharma Improved competitive ligand binding assays
EP3969907B1 (en) * 2019-05-13 2025-09-24 F. Hoffmann-La Roche AG Interference-suppressed pharmacokinetic immunoassay
CN117805397A (zh) * 2024-02-29 2024-04-02 军科正源（北京）药物研究有限责任公司 检测游离vegf的方法

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Also Published As

Publication number Publication date
EP2795335B1 (en) 2017-09-06
MX2014007095A (es) 2014-07-22
US20150072359A1 (en) 2015-03-12
JP6203747B2 (ja) 2017-09-27
KR20140099277A (ko) 2014-08-11
HK1201920A1 (zh) 2015-09-11
CN104040350A (zh) 2014-09-10
WO2013092611A3 (en) 2013-10-17
WO2013092611A2 (en) 2013-06-27
BR112014011535A2 (pt) 2017-05-09
MX349003B (es) 2017-07-06
EP2795335A2 (en) 2014-10-29
JP2015502547A (ja) 2015-01-22
RU2633488C2 (ru) 2017-10-12
CA2853917A1 (en) 2013-06-27
US9671394B2 (en) 2017-06-06
RU2014128510A (ru) 2016-02-10

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