CN103992952A - Cladosporium sp. fungi improving salvia miltiorrhiza output and total phenolic-acid content - Google Patents
Cladosporium sp. fungi improving salvia miltiorrhiza output and total phenolic-acid content Download PDFInfo
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- CN103992952A CN103992952A CN201310050802.9A CN201310050802A CN103992952A CN 103992952 A CN103992952 A CN 103992952A CN 201310050802 A CN201310050802 A CN 201310050802A CN 103992952 A CN103992952 A CN 103992952A
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- salvia miltiorrhiza
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Abstract
A cladosporium sp. fungi improving salvia miltiorrhiza output and total phenolic-acid content belongs to cladosporium sp. and has the preservation number of CGMCC No. 6608, and the name of the strain is 11673-58, and is 58# for short. When salvia miltiorrhiza is cultivated, 0.5-1.5 g of 58# fungal material is applied to the root of each salvia miltiorrhiza seedling, the fungal material and the salvia miltiorrhiza seedling are subjected to symbiosis cultivation for 4-6 months, and the 58# fungal material helps to improve the output of salvia miltiorrhiza at harvest time and the content of salvia miltiorrhiza total phenolic acids and salvianolic acid B. The technological method is simple and practicable and free of pollution, is applicable to artificial cultivation of salvia miltiorrhiza, is capable of effectively improving the output and the quality of the medicinal material, and has good application prospect.
Description
Technical field
The invention belongs to biological technical field, relate to the fungi of a strain raising red sage root output and active constituent content.
Background technology
The medicinal red sage root is the dry root and rhizome of the labiate red sage root (Salvia miltiorrhiza Bge.), Shennong's Herbal begins to be loaded in, occupy top grade, have invigorate blood circulation, effect of cool blood, the relieving restlessness that clears away heart-fire, be widely used in clinically the treatment of coronary heart disease.The base therapy medicine of red sage formulation Ye Shi China cardiovascular and cerebrovascular disease, because of determined curative effect, it is large that the red sage root has become China's consumption, the Chinese medicine that sales volume is many.
Salvianolic acid constituents is the water soluble component of the red sage root, and multiple organs such as the heart, brain, liver, kidney, stomach are had to provide protection.Salvianolic acid B is main component and activeconstituents wherein, has significant pharmacologically active at aspects such as Cardioprotective, atherosclerosis, anti-hepatic fibrosis, brain protections, is widely used in clinical.
Endogenetic fungus refers to live within plant tissue those whole or a certain periods in its life history, plant tissue is not caused to the fungi of obvious disease symptom.Research is found, is had the existence of endogenetic fungus in the histoorgan of nearly all health plant.Endogenetic fungus and plant symbiosis not only can improve the adaptive faculty of plant to environment stresses such as high temperature, arid, salinization, pathogenic bacterias, can also improve the content of active components in medicinal plant, thereby improve medical material quanlity.These of endogenetic fungus act on exploitation and the protection to resources of medicinal plant, and accumulation to active components in medicinal plant etc. has great significance.
The present invention utilizes biotechnology, separates, filters out the endogenetic fungus that can improve Radix Salviae Miltiorrhizae total phenolic acids content from the red sage root; Cultivation by bacterial strain for the red sage root, realizes and improves red sage root output harvesting time, improves the object of Radix Salviae Miltiorrhizae total phenolic acids and content of danshinolic acid B.In the exploitation of this invention to red sage root resource and protection, the red sage root, application production of liposoluble ingredient etc. all has great significance.This technological method is simple to operate, and environmental protection is with a wide range of applications, and there is not yet both at home and abroad at present and utilizes fungi and red sage root symbiotic cultivation, improves the research report of red rooted salvia output and liposoluble ingredient content.
Summary of the invention
The object of the invention is to, can improve the bacterial strain of red sage root output and liposoluble ingredient content for the artificial culture of the red sage root provides a strain.Strain name is 11673-58, hereinafter to be referred as 58#, through being accredited as a spore (Cladosporium sp.).This bacterial strain on September 26th, 2012 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, depositary institution address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, preserving number CGMCC No.6608.Bacterial strain 58# is the endogenetic fungus of a strain red sage root, through with the red sage root seedling symbiotic cultivation 4-6 month after, compare with the red sage root that does not connect bacterium under identical planting environment, can improve red rooted salvia output and liposoluble ingredient content.
To achieve these goals, the present invention takes following technology to solve:
(1) solid culture of 58#: substratum composition by weight calculating is: wheat bran: 10%-17%, wood chip: 15%-17%, KH
2pO
4: 0.2%-0.3%, MgSO
4: 0.1%-0.2%, CaSO
4: 0.07%-0.10%, glucose: 1.40%-2.20%, corn cob: 2.00%-4.00%, the broken particle of corn: 3.00%-5.00%, all the other compositions are water.Take various components, mix rear packing, 122 DEG C of sterilizing 140min, cool for subsequent use.Be taken at the 58# bacterium sheet of cultivating on PDA substratum, be placed on the middle part of solid medium, 25 DEG C of darkrooms are cultured to 58# bacterium and grow to 2/3 place of substratum in bottle and can use.
(2) vaccine symbiotic cultivation: when the spring cultivation red sage root, the red sage root seedling of 20-30 days after taking-up bud, bestow respectively the 58# bacterium material 0.5-1.5g of solid culture at every strain red sage root seedling root, after covering, plant in Nei Huo great field, greenhouse with soil, ordinary method is carried out Cultivate administration.
(3) bacterial strain 58# through with the red sage root seedling symbiotic cultivation 4-6 month after, compare red sage root dry weight, and the content of total phenolic acid and salvianolic acid B all has raising in various degree with the red sage root that does not connect bacterium under identical planting environment.
Embodiment
Embodiment 1
The solid culture of 58#: wheat bran: 100g, wood chip: 150g, KH
2pO
4: 2g, MgSO
4: 1g, CaSO
4: 0.7g, glucose: 14g, corn cob: 20g, the broken particle of corn: 30g, adds water and mixes, and is sub-packed in bottle, and 122 DEG C of sterilizing 140min, cool for subsequent use.Be taken at the 58# bacterium sheet of cultivating on PDA substratum, be placed on the middle part of solid medium, 25 DEG C of darkrooms are cultured to 58# bacterium and grow to 2/3 place of substratum in bottle and can use.
Vaccine symbiotic cultivation: by the method for greenhouse pot culture, in the time of the spring cultivation red sage root, the red sage root seedling of 20-30 days after taking-up bud, bestow respectively the 58# bacterium material 0.5g of solid culture at every strain red sage root seedling root, every basin 1 strain, potted plant in greenhouse after covering with soil, ordinary method is carried out Cultivate administration, results after 6 months.
Weigh dry weight, survey the content of total phenolic acid and salvianolic acid B.With the red sage root of 58# bacterium symbiotic cultivation, every strain root dry weight average out to 20g, the total phenolic content average out to 7.2% of root, content of danshinolic acid B average out to 2.9%.Do not connect the red sage root of bacterium, every strain root dry weight average out to 10g, the total phenolic content average out to 3.6% of root, content of danshinolic acid B average out to 2.5%.
Embodiment 2
The solid culture of 58#: wheat bran: 170g, wood chip: 170g, KH
2pO
4: 3g, MgSO
4: 2g, CaSO
4: 1g, glucose: 22g, corn cob: 40g, the broken particle of corn: 50g, adds water and mixes, and is sub-packed in bottle, and 122 DEG C of sterilizing 140min, cool for subsequent use.Be taken at the bacterium sheet 58# after purifying on PDA substratum, be placed on the middle part of solid medium, 25 DEG C of darkrooms are cultured to 58# bacterium and grow to 2/3 place of substratum in bottle and can use.
Vaccine symbiotic cultivation: by the method for field cultivation, when the spring cultivation red sage root, the red sage root seedling of 20-30 days after taking-up bud, at the 58# bacterium material 1.0g that bestows respectively solid culture at every strain red sage root seedling root, after covering with soil, plant in field, ordinary method is carried out field planting management, results after 4 months.
Survey the content of total phenolic acid and salvianolic acid B.With the red sage root of 58# bacterium symbiotic cultivation, the total phenolic content average out to 11.4% of root, content of danshinolic acid B average out to 2.8%.Do not connect the red sage root of bacterium, the total phenolic content average out to 10.5% of root, content of danshinolic acid B average out to 2.7%.
Embodiment 3
The solid culture of 58#: wheat bran: 135g, wood chip: 160g, KH
2pO
4: 2.5g, MgSO
4: 1.5g, CaSO
4: 0.85g, glucose: 18g, corn cob: 30g, the broken particle of corn: 40g, adds water and mixes, and is sub-packed in bottle, and 122 DEG C of sterilizing 140min, cool for subsequent use.Be taken at the 58# bacterium sheet of cultivating on PDA substratum, be placed on the middle part of solid medium, 25 DEG C of darkrooms are cultured to 58# bacterium and grow to 2/3 place of substratum in bottle and can use.
Vaccine symbiotic cultivation: by the method for field cultivation, when the spring cultivation red sage root, the salvia seeds seedling of 20-30 days after taking-up bud, bestow respectively the 58# bacterium material 1.5g of solid culture at every strain red sage root seedling root, after covering with soil, plant in field, ordinary method is carried out field planting management, results after 6 months.
Weigh dry weight, survey the content of total phenolic acid and salvianolic acid B.With the red sage root of 58# bacterium symbiotic cultivation, every strain root dry weight average out to 26g, the total phenolic content average out to 6.3% of root, content of danshinolic acid B average out to 2.3%.Do not connect the red sage root of bacterium, every strain root dry weight average out to 15g, the total phenolic content average out to 4.3% of root, content of danshinolic acid B average out to 2.4%.
Claims (3)
1. improve a fungal strain of red sage root output and liposoluble ingredient content, it is that deposit number is the branch spore (Cladosporium sp.) of CGMCC No.6608, and strain name: 11673-58, is called for short 58#; This bacterial strain can use in the time of planted rooted salvia.
2. fungi as claimed in claim 1, the nutrient media components of its solid culture is by weight: wheat bran: 10%-17%, wood chip: 15%-17%, KH
2pO
4: 0.2%-0.3%, MgSO
4: 0.1%-0.2%, CaSO
4: 0.07%-0.10%, glucose: 1.40%-2.20%, corn cob: 2.00%-4.00%, the broken particle of corn: 3.00%-5.00%, all the other compositions are water.
3. fungi as claimed in claim 1, its using method is: when planted rooted salvia, the red sage root seedling of 20-30 days after taking-up bud, bestow respectively the 58# bacterium material 0.5-1.5g of solid culture at every strain red sage root seedling root, with soil covering, plant in Nei Huo great field, greenhouse, regularly execute water, ordinary method is carried out Cultivate administration, results after 4-6 month.
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| CN201310050802.9A CN103992952B (en) | 2013-02-16 | 2013-02-16 | One strain improves Radix Salviae Miltiorrhizae yield and the Cladosporium fungus of total phenolics content |
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| CN201310050802.9A CN103992952B (en) | 2013-02-16 | 2013-02-16 | One strain improves Radix Salviae Miltiorrhizae yield and the Cladosporium fungus of total phenolics content |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110438011A (en) * | 2019-07-30 | 2019-11-12 | 浙江理工大学 | Superfine branch spore is mould and its promotes the purposes of Radix Salviae Miltiorrhizae root system effective component synthesis |
| CN112273171A (en) * | 2016-04-28 | 2021-01-29 | 首尔伟傲世有限公司 | Growth and bioactive substance promoting system for medicinal crops |
| CN112369435A (en) * | 2020-12-07 | 2021-02-19 | 中南林业科技大学 | Salvia miltiorrhiza growth regulating bacteria suspension and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102428871A (en) * | 2011-09-24 | 2012-05-02 | 西北农林科技大学 | Method for improving yield of salvianolic acid B in savia miltiorrhiza suspension culture cells by inducing |
| CN102676392A (en) * | 2011-04-08 | 2012-09-19 | 中国人民解放军第二军医大学 | Endophytic fungus in salvia miltiorrhiza bunge and application thereof |
-
2013
- 2013-02-16 CN CN201310050802.9A patent/CN103992952B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102676392A (en) * | 2011-04-08 | 2012-09-19 | 中国人民解放军第二军医大学 | Endophytic fungus in salvia miltiorrhiza bunge and application thereof |
| CN102428871A (en) * | 2011-09-24 | 2012-05-02 | 西北农林科技大学 | Method for improving yield of salvianolic acid B in savia miltiorrhiza suspension culture cells by inducing |
Non-Patent Citations (3)
| Title |
|---|
| 冀玉良 等: "商洛丹参内生真菌的种群多样性研究", 《安徽农业科学》 * |
| 李艳玲 等: "泰山产丹参内生真菌的分离鉴定和多样性分析", 《时珍国医国药》 * |
| 沈双: "诱导子对丹参毛状根生长和丹参酮含量的影响", 《中国优秀硕士学位论文全文数据库•农业科技辑》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112273171A (en) * | 2016-04-28 | 2021-01-29 | 首尔伟傲世有限公司 | Growth and bioactive substance promoting system for medicinal crops |
| CN110438011A (en) * | 2019-07-30 | 2019-11-12 | 浙江理工大学 | Superfine branch spore is mould and its promotes the purposes of Radix Salviae Miltiorrhizae root system effective component synthesis |
| CN112369435A (en) * | 2020-12-07 | 2021-02-19 | 中南林业科技大学 | Salvia miltiorrhiza growth regulating bacteria suspension and preparation method and application thereof |
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| CN103992952B (en) | 2016-12-28 |
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