CN103992961B - One strain improves Radix Salviae Miltiorrhizae yield and the paecilomyces fungus of total phenolics content - Google Patents
One strain improves Radix Salviae Miltiorrhizae yield and the paecilomyces fungus of total phenolics content Download PDFInfo
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- CN103992961B CN103992961B CN201310050788.2A CN201310050788A CN103992961B CN 103992961 B CN103992961 B CN 103992961B CN 201310050788 A CN201310050788 A CN 201310050788A CN 103992961 B CN103992961 B CN 103992961B
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Abstract
One strain improves Radix Salviae Miltiorrhizae yield and the fungus of liposoluble ingredient content, it be deposit number be the Paecilomyces varioti (Paecilomyces sp.) of CGMCC No.6627, strain name: 11,736 34, be called for short 34#.Bestow 34# bacterium material 0.5 1.5g of solid culture during planted rooted salvia respectively at every strain Radix Salviae Miltiorrhizae seedling root, through with Radix Salviae Miltiorrhizae seedling symbiotic cultivation 4 June, the yield of Radix Salviae Miltiorrhizae and Radix Salviae Miltiorrhizae total phenolic acids and the content of salvianolic acid B when can improve harvest time.This technical method is simple, pollution-free, for the artificial growth of Radix Salviae Miltiorrhizae, can be effectively improved yield and the quality of medical material, have a good application prospect.
Description
Technical field
The invention belongs to biological technical field, relate to a strain and improve Radix Salviae Miltiorrhizae yield and the fungus of active constituent content.
Background technology
Medicinal Radix Salviae Miltiorrhizae is the dry root and rhizome of labiate Radix Salviae Miltiorrhizae (Salvia miltiorrhiza Bge.), begins to carry
In Shennong's Herbal, occupy top grade, have invigorate blood circulation, removing heat from blood, the effect of the relieving restlessness that clears away heart-fire, be widely used in controlling of coronary heart disease clinically
Treat.The base therapy medicine of red sage formulation Ye Shi China cardiovascular and cerebrovascular disease, because of determined curative effect, it is big that Radix Salviae Miltiorrhizae has become China's consumption,
The Chinese medicine that sales volume is many.
Salvianolic acid constituents is the water soluble ingredient of Radix Salviae Miltiorrhizae, has protection to multiple organs such as the heart, brain, liver, kidney, stomaches and makees
With.Salvianolic acid B is main component therein and active component, at Cardioprotective, atherosclerosis, anti-hepatic fibrosis, brain
The aspects such as protection have significant pharmacologically active, are widely used in clinic.
Endogenetic fungus refers to that those are lived within plant tissue the whole of its life cycle or a certain period, does not has plant tissue
There is the fungus causing obvious Disease symptoms.Research finds, has endogenetic fungus in the histoorgan of nearly all health plant
Exist.Endogenetic fungus and plant symbiosis are possible not only to improve plant and fit the environment stresses such as high temperature, arid, salinization, pathogen
Should be able to power, it is also possible to improve the content of active components in medicinal plant, thus improve medical material quanlity.These effects of endogenetic fungus are right
The exploitation of resources of medicinal plant and protection, and the accumulation etc. to active components in medicinal plant has great significance.
The present invention utilizes biotechnology, separates, filters out the Nei Shengzhen that can improve Radix Salviae Miltiorrhizae total phenolic acids content from Radix Salviae Miltiorrhizae
Bacterium；Bacterial strain is used in the cultivation of Radix Salviae Miltiorrhizae, it is achieved improve Radix Salviae Miltiorrhizae yield harvest time, and improve Radix Salviae Miltiorrhizae total phenolic acids and salvianolic acid B
The purpose of content.This invention suffers from weight to the exploitation of Radix Salviae Miltiorrhizae resource with the application production etc. of liposoluble ingredient in protection, Radix Salviae Miltiorrhizae
The meaning wanted.This technical method is simple to operate, and environmental protection is with a wide range of applications, and there is not yet utilization the most both at home and abroad
Fungus and Radix Salviae Miltiorrhizae symbiotic cultivation, improve red rooted salvia yield and the research report of liposoluble ingredient content.
Summary of the invention
It is an object of the invention to, the artificial culture for Radix Salviae Miltiorrhizae provides a strain can improve Radix Salviae Miltiorrhizae yield and liposoluble ingredient contains
The bacterial strain of amount.Strain name is 11736-34, hereinafter referred to as 34#, is identified as Paecilomyces varioti (Paecilomyces sp.).This bacterium
Strain JIUYUE in 2012 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on the 26th, depositary institution address:
Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, preserving number CGMCC No.6627.Bacterial strain 34# is the endogenetic fungus of a strain Radix Salviae Miltiorrhizae,
Through with the Radix Salviae Miltiorrhizae seedling symbiotic cultivation 4-6 month after, compared with the Radix Salviae Miltiorrhizae not connecing bacterium under identical planting environment, can improve red rooted salvia produce
Amount and liposoluble ingredient content.
To achieve these goals, the present invention takes following technology:
(1) solid culture of 34#: culture medium composition by weight calculating is: Testa Tritici: 10%-17%, wood flour: 15%-
17%, KH2PO4: 0.2%-0.3%, MgSO4: 0.1%-0.2%, CaSO4: 0.07%-0.10%, glucose: 1.40%-
2.20%, corn cob: 2.00%-4.00%, the broken granule of Semen Maydis: 3.00%-5.00%, remaining composition is water.Weigh various groups
Point, subpackage after mixing, 122 DEG C of sterilizing 140min, cool standby.It is taken in PDA culture medium the 34# bacterium sheet cultivated, is placed on solid
The middle part of body culture medium, 25 DEG C of darkroom cultivations can use in bottle the 2/3 of culture medium to 34# bacterium length.
(2) vaccine symbiotic cultivation: during spring cultivation Radix Salviae Miltiorrhizae, the Radix Salviae Miltiorrhizae Seedling of 20-30 days after taking-up bud, every strain Radix Salviae Miltiorrhizae seedling
Root bestows the 34# bacterium material 0.5-1.5g of solid culture respectively, plants in Nei Huo great field, greenhouse after covering with soil, and conventional method is entered
Row Cultivate administration.
(3) bacterial strain 34# through with the Radix Salviae Miltiorrhizae seedling symbiotic cultivation 4-6 month after, do not connect the Radix Salviae Miltiorrhizae phase of bacterium under identical planting environment
Ratio, Radix Salviae Miltiorrhizae dry weight, and the content of total phenolics and salvianolic acid B all have raising in various degree.
Detailed description of the invention
Embodiment 1
The solid culture of 34#: Testa Tritici: 100g, wood flour: 150g, KH2PO4: 2g, MgSO4: 1g, CaSO4: 0.7g, Fructus Vitis viniferae
Sugar: 14g, corn cob: 20g, the broken granule of Semen Maydis: 30g, add water mix homogeneously, is sub-packed in bottle, 122 DEG C of sterilizing 140min, cools
Standby.Being taken in PDA culture medium the 34# bacterium sheet cultivated, be placed on the middle part of solid medium, 25 DEG C of darkrooms are cultivated to 34# bacterium
Length can use in bottle the 2/3 of culture medium.
Vaccine symbiotic cultivation: by the method for greenhouse pot culture, when spring cultivation Radix Salviae Miltiorrhizae, the Radix Salviae Miltiorrhizae of 20-30 days after taking-up bud
Seedling, bestows the 34# bacterium material 0.5g of solid culture respectively at every strain Radix Salviae Miltiorrhizae seedling root, and the strain of every basin 1, with potted plant in temperature after soil covering
In room, conventional method carries out Cultivate administration, gathers in the crops after 6 months.
Weigh dry weight, survey total phenolics and the content of salvianolic acid B.With the Radix Salviae Miltiorrhizae of 34# bacterium symbiotic cultivation, every strain root dry weight is average
For 17g, root total phenolics content average out to 6.8%, content of danshinolic acid B average out to 2.8%.Not connecing the Radix Salviae Miltiorrhizae of bacterium, every strain root is done
Galassing is 10g, root total phenolics content average out to 3.6%, content of danshinolic acid B average out to 2.5%.
Embodiment 2
The solid culture of 34#: Testa Tritici: 170g, wood flour: 170g, KH2PO4: 3g, MgSO4: 2g, CaSO4: 1g, glucose:
22g, corn cob: 40g, the broken granule of Semen Maydis: 50g, add water mix homogeneously, is sub-packed in bottle, 122 DEG C of sterilizing 140min, cools standby
With.Being taken in PDA culture medium bacterium sheet 34# after purification, be placed on the middle part of solid medium, 25 DEG C of darkrooms are cultivated to 34# bacterium
Length can use in bottle the 2/3 of culture medium.
Vaccine symbiotic cultivation: by the method for field cultivation, during spring cultivation Radix Salviae Miltiorrhizae, the Radix Salviae Miltiorrhizae kind of 20-30 days after taking-up bud
Sub-Seedling, bestows the 34# bacterium material 1.0g of solid culture respectively at every strain Radix Salviae Miltiorrhizae seedling root, plants in field after covering with soil, conventional
Method carries out field planting management, gathers in the crops after 4 months.
Survey total phenolics and the content of salvianolic acid B.With the Radix Salviae Miltiorrhizae of 34# bacterium symbiotic cultivation, root total phenolics content average out to
12.4%, content of danshinolic acid B average out to 5.8%.Do not connect the Radix Salviae Miltiorrhizae of bacterium, root total phenolics content average out to 10.5%, salvianolic acid B
Content average out to 2.7%.
Embodiment 3
The solid culture of 34#: Testa Tritici: 135g, wood flour: 160g, KH2PO4: 2.5g, MgSO4: 1.5g, CaSO4: 0.85g,
Glucose: 18g, corn cob: 30g, the broken granule of Semen Maydis: 40g, add water mix homogeneously, is sub-packed in bottle, 122 DEG C of sterilizing 140min,
Cool standby.Being taken in PDA culture medium the 34# bacterium sheet cultivated, be placed on the middle part of solid medium, 25 DEG C of darkrooms are cultivated extremely
34# bacterium length can use in bottle the 2/3 of culture medium.
Vaccine symbiotic cultivation: by the method for field cultivation, during spring cultivation Radix Salviae Miltiorrhizae, the Radix Salviae Miltiorrhizae kind of 20-30 days after taking-up bud
Sub-Seedling, bestows the 34# bacterium material 1.5g of solid culture respectively at every strain Radix Salviae Miltiorrhizae seedling root, plants in field after covering with soil, conventional
Method carries out field planting management, gathers in the crops after 6 months.
Weigh dry weight, survey total phenolics and the content of salvianolic acid B.With the Radix Salviae Miltiorrhizae of 34# bacterium symbiotic cultivation, every strain root dry weight is average
For 20g, root total phenolics content average out to 8.7%, content of danshinolic acid B average out to 4.2%.Not connecing the Radix Salviae Miltiorrhizae of bacterium, every strain root is done
Galassing is 15g, root total phenolics content average out to 4.3%, content of danshinolic acid B average out to 2.4%.
Claims (3)
1. improve Radix Salviae Miltiorrhizae yield and a fungal strain of liposoluble ingredient content, it be deposit number be the plan of CGMCC No.6627
Penicillium sp (Paecilomyces sp.), strain name: 11736-34, is called for short 34#；This bacterial strain can use when planted rooted salvia.
2. fungus as claimed in claim 1, the nutrient media components of its solid culture by weight: Testa Tritici: 10%-17%,
Wood flour: 15%-17%, KH2PO4: 0.2%-0.3%, MgSO4: 0.1%-0.2%, CaSO4: 0.07%-0.10%, Fructus Vitis viniferae
Sugar: 1.40%-2.20%, corn cob: 2.00%-4.00%, the broken granule of Semen Maydis: 3.00%-5.00%, remaining composition is water.
3. fungus as claimed in claim 1, its using method is: during planted rooted salvia, the Radix Salviae Miltiorrhizae Seedling of 20-30 days after taking-up bud,
Bestow the 34# bacterium material 0.5-1.5g of solid culture at every strain Radix Salviae Miltiorrhizae seedling root respectively, cover with soil, plant in greenhouse or land for growing field crops
In, periodically execute water, conventional method carries out Cultivate administration, gathers in the crops after 4-6 month.
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| CN112369435B (en) * | 2020-12-07 | 2021-10-08 | 中南林业科技大学 | Salvia miltiorrhiza growth regulating bacteria suspension and preparation method and application thereof |
| CN115197851B (en) * | 2022-05-30 | 2023-07-21 | 浙江理工大学绍兴生物医药研究院有限公司 | Penicillium bifidum and application thereof in regulating and controlling synthesis of tanshinone components in root of salvia miltiorrhiza |
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| CN102428871A (en) * | 2011-09-24 | 2012-05-02 | 西北农林科技大学 | Method for improving yield of salvianolic acid B in savia miltiorrhiza suspension culture cells by inducing |
| CN102676392A (en) * | 2011-04-08 | 2012-09-19 | 中国人民解放军第二军医大学 | Endophytic fungus in salvia miltiorrhiza bunge and application thereof |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102676392A (en) * | 2011-04-08 | 2012-09-19 | 中国人民解放军第二军医大学 | Endophytic fungus in salvia miltiorrhiza bunge and application thereof |
| CN102428871A (en) * | 2011-09-24 | 2012-05-02 | 西北农林科技大学 | Method for improving yield of salvianolic acid B in savia miltiorrhiza suspension culture cells by inducing |
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