CN103992961A - Paecilomyces sp. fungi improving salvia miltiorrhiza output and total phenolic-acid content - Google Patents
Paecilomyces sp. fungi improving salvia miltiorrhiza output and total phenolic-acid content Download PDFInfo
- Publication number
- CN103992961A CN103992961A CN201310050788.2A CN201310050788A CN103992961A CN 103992961 A CN103992961 A CN 103992961A CN 201310050788 A CN201310050788 A CN 201310050788A CN 103992961 A CN103992961 A CN 103992961A
- Authority
- CN
- China
- Prior art keywords
- red sage
- salvia miltiorrhiza
- sage root
- strain
- fungi
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
A paecilomyces sp. fungi improving salvia miltiorrhiza output and total phenolic-acid content belongs to cladosporium sp. and has the preservation number of CGMCC No. 6627, and the name of the strain is 11736-34, and is 34# for short. When salvia miltiorrhiza is cultivated, 0.5-1.5 g of 34# fungal material is applied to the root of each salvia miltiorrhiza seedling, the fungal material and the salvia miltiorrhiza seedling are subjected to symbiosis cultivation for 4-6 months, and the 34# fungal material helps to improve the output of salvia miltiorrhiza at harvest time and the content of salvia miltiorrhiza total phenolic acids and salvianolic acid B. The technological method is simple and practicable and free of pollution, is applicable to artificial cultivation of salvia miltiorrhiza, is capable of effectively improving the output and the quality of the medicinal material, and has good application prospect.
Description
Technical field
The invention belongs to biological technical field, relate to the fungi of a strain raising red sage root output and active constituent content.
Background technology
The medicinal red sage root is the dry root and rhizome of the labiate red sage root (Salvia mil tiorrhiza Bge.), Shennong's Herbal begins to be loaded in, occupy top grade, have invigorate blood circulation, effect of cool blood, the relieving restlessness that clears away heart-fire, be widely used in clinically the treatment of coronary heart disease.The base therapy medicine of red sage formulation Ye Shi China cardiovascular and cerebrovascular disease, because of determined curative effect, it is large that the red sage root has become China's consumption, the Chinese medicine that sales volume is many.
Salvianolic acid constituents is the water soluble component of the red sage root, and multiple organs such as the heart, brain, liver, kidney, stomach are had to provide protection.Salvianolic acid B is main component and activeconstituents wherein, has significant pharmacologically active at aspects such as Cardioprotective, atherosclerosis, anti-hepatic fibrosis, brain protections, is widely used in clinical.
Endogenetic fungus refers to live within plant tissue those whole or a certain periods in its life history, plant tissue is not caused to the fungi of obvious disease symptom.Research is found, is had the existence of endogenetic fungus in the histoorgan of nearly all health plant.Endogenetic fungus and plant symbiosis not only can improve the adaptive faculty of plant to environment stresses such as high temperature, arid, salinization, pathogenic bacterias, can also improve the content of active components in medicinal plant, thereby improve medical material quanlity.These of endogenetic fungus act on exploitation and the protection to resources of medicinal plant, and accumulation to active components in medicinal plant etc. has great significance.
The present invention utilizes biotechnology, separates, filters out the endogenetic fungus that can improve Radix Salviae Miltiorrhizae total phenolic acids content from the red sage root; Cultivation by bacterial strain for the red sage root, realizes and improves red sage root output harvesting time, and improves the object of Radix Salviae Miltiorrhizae total phenolic acids and content of danshinolic acid B.In the exploitation of this invention to red sage root resource and protection, the red sage root, application production of liposoluble ingredient etc. all has great significance.This technological method is simple to operate, and environmental protection is with a wide range of applications, and there is not yet both at home and abroad at present and utilizes fungi and red sage root symbiotic cultivation, improves the research report of red rooted salvia output and liposoluble ingredient content.
Summary of the invention
The object of the invention is to, can improve the bacterial strain of red sage root output and liposoluble ingredient content for the artificial culture of the red sage root provides a strain.Strain name is 11736-34, hereinafter to be referred as 34#, through being accredited as Paecilomyces varioti (Paecilomyces sp.).This bacterial strain on September 26th, 2012 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, depositary institution address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, preserving number CGMCC No.6627.Bacterial strain 34# is the endogenetic fungus of a strain red sage root, through with the red sage root seedling symbiotic cultivation 4-6 month after, compare with the red sage root that does not connect bacterium under identical planting environment, can improve red rooted salvia output and liposoluble ingredient content.
To achieve these goals, the present invention takes following technology:
(1) solid culture of 34#: substratum composition by weight calculating is: wheat bran: 10%-17%, wood chip: 15%-17%, KH
2pO
4: 0.2%-0.3%, MgSO
4: 0.1%-0.2%, CaSO
4: 0.07%-0.10%, glucose: 1.40%-2.20%, corn cob: 2.00%-4.00%, the broken particle of corn: 3.00%-5.00%, all the other compositions are water.Take various components, mix rear packing, 122 DEG C of sterilizing 140min, cool for subsequent use.Be taken at the 34# bacterium sheet of cultivating on PDA substratum, be placed on the middle part of solid medium, 25 DEG C of darkrooms are cultured to 34# bacterium and grow to 2/3 place of substratum in bottle and can use.
(2) vaccine symbiotic cultivation: when the spring cultivation red sage root, the red sage root seedling of 20-30 days after taking-up bud, bestow respectively the 34# bacterium material 0.5-1.5g of solid culture at every strain red sage root seedling root, after covering, plant in Nei Huo great field, greenhouse with soil, ordinary method is carried out Cultivate administration.
(3) bacterial strain 34# through with the red sage root seedling symbiotic cultivation 4-6 month after, compare red sage root dry weight, and the content of total phenolic acid and salvianolic acid B all has raising in various degree with the red sage root that does not connect bacterium under identical planting environment.
Embodiment
Embodiment 1
The solid culture of 34#: wheat bran: 100g, wood chip: 150g, KH
2pO
4: 2g, MgSO
4: 1g, CaSO
4: 0.7g, glucose: 14g, corn cob: 20g, the broken particle of corn: 30g, adds water and mixes, and is sub-packed in bottle, and 122 DEG C of sterilizing 140min, cool for subsequent use.Be taken at the 34# bacterium sheet of cultivating on PDA substratum, be placed on the middle part of solid medium, 25 DEG C of darkrooms are cultured to 34# bacterium and grow to 2/3 place of substratum in bottle and can use.
Vaccine symbiotic cultivation: by the method for greenhouse pot culture, in the time of the spring cultivation red sage root, the red sage root seedling of 20-30 days after taking-up bud, bestow respectively the 34# bacterium material 0.5g of solid culture at every strain red sage root seedling root, every basin 1 strain, potted plant in greenhouse after covering with soil, ordinary method is carried out Cultivate administration, results after 6 months.
Weigh dry weight, survey the content of total phenolic acid and salvianolic acid B.With the red sage root of 34# bacterium symbiotic cultivation, every strain root dry weight average out to 17g, the total phenolic content average out to 6.8% of root, content of danshinolic acid B average out to 2.8%.Do not connect the red sage root of bacterium, every strain root dry weight average out to 10g, the total phenolic content average out to 3.6% of root, content of danshinolic acid B average out to 2.5%.
Embodiment 2
The solid culture of 34#: wheat bran: 170g, wood chip: 170g, KH
2pO
4: 3g, MgSO
4: 2g, CaSO
4: 1g, glucose: 22g, corn cob: 40g, the broken particle of corn: 50g, adds water and mixes, and is sub-packed in bottle, and 122 DEG C of sterilizing 140min, cool for subsequent use.Be taken at the bacterium sheet 34# after purifying on PDA substratum, be placed on the middle part of solid medium, 25 DEG C of darkrooms are cultured to 34# bacterium and grow to 2/3 place of substratum in bottle and can use.
Vaccine symbiotic cultivation: by the method for field cultivation, when the spring cultivation red sage root, the salvia seeds seedling of 20-30 days after taking-up bud, bestow respectively the 34# bacterium material 1.0g of solid culture at every strain red sage root seedling root, after covering with soil, plant in field, ordinary method is carried out field planting management, results after 4 months.
Survey the content of total phenolic acid and salvianolic acid B.With the red sage root of 34# bacterium symbiotic cultivation, the total phenolic content average out to 12.4% of root, content of danshinolic acid B average out to 5.8%.Do not connect the red sage root of bacterium, the total phenolic content average out to 10.5% of root, content of danshinolic acid B average out to 2.7%.
Embodiment 3
The solid culture of 34#: wheat bran: 135g, wood chip: 160g, KH
2pO
4: 2.5g, MgSO
4: 1.5g, CaSO
4: 0.85g, glucose: 18g, corn cob: 30g, the broken particle of corn: 40g, adds water and mixes, and is sub-packed in bottle, and 122 DEG C of sterilizing 140min, cool for subsequent use.Be taken at the 34# bacterium sheet of cultivating on PDA substratum, be placed on the middle part of solid medium, 25 DEG C of darkrooms are cultured to 34# bacterium and grow to 2/3 place of substratum in bottle and can use.
Vaccine symbiotic cultivation: by the method for field cultivation, when the spring cultivation red sage root, the salvia seeds seedling of 20-30 days after taking-up bud, bestow respectively the 34# bacterium material 1.5g of solid culture at every strain red sage root seedling root, after covering with soil, plant in field, ordinary method is carried out field planting management, results after 6 months.
Weigh dry weight, survey the content of total phenolic acid and salvianolic acid B.With the red sage root of 34# bacterium symbiotic cultivation, every strain root dry weight average out to 20g, the total phenolic content average out to 8.7% of root, content of danshinolic acid B average out to 4.2%.Do not connect the red sage root of bacterium, every strain root dry weight average out to 15g, the total phenolic content average out to 4.3% of root, content of danshinolic acid B average out to 2.4%.
Claims (3)
1. a fungal strain that improves red sage root output and liposoluble ingredient content, it is that deposit number is the Paecilomyces varioti (Paecilomyces sp.) of CGMCC No.6627, strain name: 11736-34 is called for short 34#; This bacterial strain can use in the time of planted rooted salvia.
2. fungi as claimed in claim 1, the nutrient media components of its solid culture is by weight: wheat bran: 10%-17%, wood chip: 15%-17%, KH
2pO
4: 0.2%-0.3%, MgSO
4: 0.1%-0.2%, CaSO
4: 0.07%-0.10%, glucose: 1.40%-2.20%, corn cob: 2.00%-4.00%, the broken particle of corn: 3.00%-5.00%, all the other compositions are water.
3. fungi as claimed in claim 1, its using method is: when planted rooted salvia, the red sage root seedling of 20-30 days after taking-up bud, bestow respectively the 34# bacterium material 0.5-1.5g of solid culture at every strain red sage root seedling root, with soil covering, plant in Nei Huo great field, greenhouse, regularly execute water, ordinary method is carried out Cultivate administration, results after 4-6 month.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310050788.2A CN103992961B (en) | 2013-02-16 | 2013-02-16 | One strain improves Radix Salviae Miltiorrhizae yield and the paecilomyces fungus of total phenolics content |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310050788.2A CN103992961B (en) | 2013-02-16 | 2013-02-16 | One strain improves Radix Salviae Miltiorrhizae yield and the paecilomyces fungus of total phenolics content |
Publications (2)
Publication Number | Publication Date |
---|---|
CN103992961A true CN103992961A (en) | 2014-08-20 |
CN103992961B CN103992961B (en) | 2016-12-28 |
Family
ID=51307298
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201310050788.2A Expired - Fee Related CN103992961B (en) | 2013-02-16 | 2013-02-16 | One strain improves Radix Salviae Miltiorrhizae yield and the paecilomyces fungus of total phenolics content |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN103992961B (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112369435A (en) * | 2020-12-07 | 2021-02-19 | 中南林业科技大学 | Salvia miltiorrhiza growth regulating bacteria suspension and preparation method and application thereof |
CN115197851A (en) * | 2022-05-30 | 2022-10-18 | 浙江理工大学绍兴生物医药研究院有限公司 | Penicillium cremorifolium and application thereof in regulating synthesis of tanshinone components at roots of salvia miltiorrhiza |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102428871A (en) * | 2011-09-24 | 2012-05-02 | 西北农林科技大学 | Method for improving yield of salvianolic acid B in savia miltiorrhiza suspension culture cells by inducing |
CN102676392A (en) * | 2011-04-08 | 2012-09-19 | 中国人民解放军第二军医大学 | Endophytic fungus in salvia miltiorrhiza bunge and application thereof |
-
2013
- 2013-02-16 CN CN201310050788.2A patent/CN103992961B/en not_active Expired - Fee Related
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102676392A (en) * | 2011-04-08 | 2012-09-19 | 中国人民解放军第二军医大学 | Endophytic fungus in salvia miltiorrhiza bunge and application thereof |
CN102428871A (en) * | 2011-09-24 | 2012-05-02 | 西北农林科技大学 | Method for improving yield of salvianolic acid B in savia miltiorrhiza suspension culture cells by inducing |
Non-Patent Citations (5)
Title |
---|
冀玉良 等: "商洛丹参内生真菌的种群多样性研究", 《安徽农业科学》, vol. 39, no. 15, 31 December 2011 (2011-12-31), pages 8913 - 8915 * |
唐坤 等: "一株促进丹参生长和提高丹酚酸含量的活性内生真菌", 《菌物学报》, vol. 33, no. 3, 15 May 2014 (2014-05-15), pages 594 - 600 * |
戴国君: "丹参内生真菌群落结构研究", 《中国优秀硕士学位论文全文数据库·农业科技辑》, 15 December 2011 (2011-12-15) * |
李艳玲 等: "泰山产丹参内生真菌的分离鉴定和多样性分析", 《时珍国医国药》, vol. 23, no. 1, 31 December 2012 (2012-12-31), pages 114 - 117 * |
沈双: "诱导子对丹参毛状根生长和丹参酮含量的影响", 《中国优秀硕士学位论文全文数据库·农业科技辑》, 15 May 2012 (2012-05-15) * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112369435A (en) * | 2020-12-07 | 2021-02-19 | 中南林业科技大学 | Salvia miltiorrhiza growth regulating bacteria suspension and preparation method and application thereof |
CN112369435B (en) * | 2020-12-07 | 2021-10-08 | 中南林业科技大学 | Salvia miltiorrhiza growth regulating bacteria suspension and preparation method and application thereof |
CN115197851A (en) * | 2022-05-30 | 2022-10-18 | 浙江理工大学绍兴生物医药研究院有限公司 | Penicillium cremorifolium and application thereof in regulating synthesis of tanshinone components at roots of salvia miltiorrhiza |
CN115197851B (en) * | 2022-05-30 | 2023-07-21 | 浙江理工大学绍兴生物医药研究院有限公司 | Penicillium bifidum and application thereof in regulating and controlling synthesis of tanshinone components in root of salvia miltiorrhiza |
Also Published As
Publication number | Publication date |
---|---|
CN103992961B (en) | 2016-12-28 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN105000994B (en) | A kind of fertilizer containing oligosaccharide and preparation method thereof | |
CN102491853A (en) | High-efficiency hardening-resisting continuous-cropping-resisting soil-modifying biological agent | |
CN101803620A (en) | Plant active glycoside peptide fungicide and preparation method thereof | |
CN104130068B (en) | A kind of composite multi-functional biological foliage fertilizer | |
CN101781569A (en) | Soil improvement and anti continuous cropping multifunctional biological agent | |
CN103224433A (en) | Soil fertility improver produced by utilizing waste bacterial residues | |
CN105198583A (en) | Nutrient medium for soilless culture | |
CN103288529A (en) | Mushroom culture material and preparation method thereof | |
CN106007824B (en) | Composite bacterial fertilizer and preparation method and application thereof | |
CN101781565B (en) | Multifunctional biological agent containing Trichoderma pseudokiningii | |
CN106946603A (en) | A kind of oil tree peony fertilizer special for organic and preparation method thereof | |
Badar et al. | Use of Trichoderma hamatum alone and in combination with rhizobial isolates as bio-fertilizer for improving the growth and strength of sunflower | |
CN103992952B (en) | One strain improves Radix Salviae Miltiorrhizae yield and the Cladosporium fungus of total phenolics content | |
CN102219613A (en) | Green multifunctional compound ecological organic liquid fertilizer and processing method thereof | |
CN103288559A (en) | Biological composite bacterial fertilizer special for salvia miltiorrhiza | |
CN102786352B (en) | Multifunctional organic liquid fertilizer and processing method thereof | |
CN103992961B (en) | One strain improves Radix Salviae Miltiorrhizae yield and the paecilomyces fungus of total phenolics content | |
ChAnDA et al. | The potential use of arbuscular mycorrhiza in the cultivation of medicinal plants in Barak Valley, Assam: A Review | |
CN103992951B (en) | One strain improves Radix Salviae Miltiorrhizae yield and the alternaria nees fungus of total phenolics content | |
CN109438107A (en) | A kind of dragon fruit bio-organic fertilizer special material and preparation method thereof | |
CN102250784B (en) | Angelica root rhizosphere efficient phosphate-solubilizing bacterium and microbial inoculum prepared from same and application thereof | |
CN104876731A (en) | Potting media additive and preparation method | |
CN104761378A (en) | Bacterial manure synergist and use method thereof | |
CN101263813B (en) | Application of oroxylin A as biological pesticide in preventing and controlling corps diseases | |
CN115380925B (en) | Composition for preventing and controlling root rot of szechuan radix codonopsis and application thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20161228 Termination date: 20220216 |