CN103990179B - 一种制备异种脱细胞基质的方法及其产品 - Google Patents
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Abstract
Description
项目 | MES(mol/L) | 每克脱细胞组织对应DCC(g) | 每克脱细胞组织对应NHS(g) | 交联时间 | 交联效果 |
实施例1 | 0.06 | 1.8 | 0.415 | 4 | 交联前后质量损失15% |
对比例4 | 0.06 | 1.8 | - | 4 | 很差 |
对比例5 | 0.06 | 0.6 | 0.415 | 4 | 交联前后质量损失70% |
对比例6 | 0.06 | 1.0 | 0.415 | 4 | 交联前后质量损失41% |
对比例7 | 0.06 | 5.5 | 0.415 | 4 | 交联前后质量损失12% |
对比例8 | 0.06 | 7.0 | 0.415 | 4 | 交联前后质量损失11% |
对比例9 | 0.06 | 1.8 | 0.310 | 4 | 交联不完全 |
对比例10 | 0.06 | 1.8 | 0.200 | 4 | 交联不完全 |
对比例11 | 0.06 | 1.8 | 1.250 | 4 | 干扰交联反应 |
对比例12 | 0.06 | 1.8 | 1.800 | 4 | 干扰交联反应 |
对比例13 | - | 1.8 | 0.415 | 4 | 很差 |
指标 | 实施例1 | 对比例1 | 对比例2 | 对比例3 |
后处理时间 | 2天 | 5天 | 7天 | 5天 |
交联时间 | 4小时 | 4小时 | 24小时 | 4小时 |
孔径大小 | 150μM | 70μM | 140μM | 30μM |
性状 | 疏松 | 较疏松 | 疏松 | 致密 |
脱细胞效果 | 完全 | 完全 | 完全 | 不完全 |
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CN104491927B (zh) * | 2014-11-19 | 2016-10-05 | 中山大学 | 一种脱细胞舌基质材料及其制备方法 |
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CN111359015A (zh) * | 2020-03-13 | 2020-07-03 | 青岛海洋生物医药研究院 | 一种降低辐照灭菌对脱细胞组织破坏作用的方法 |
CN112294849B (zh) * | 2020-11-04 | 2024-05-03 | 陕西中鸿科瑞再生医学研究院有限公司 | 用于生发的脱细胞基质-细胞复合颗粒及制备方法 |
CN113827785B (zh) * | 2021-10-15 | 2022-11-18 | 博纳格科技(天津)有限公司 | 一种可吸收生物膜的制备方法 |
CN115970059A (zh) * | 2022-07-21 | 2023-04-18 | 上海瑄元生物科技有限公司 | 含有类弹性蛋白多肽和脱细胞真皮基质的组合物及其用途 |
CN115990290A (zh) * | 2023-03-23 | 2023-04-21 | 北赛泓升(北京)生物科技有限公司 | 鸡冠油组织脱细胞基质材料及其制备方法 |
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CN100415309C (zh) * | 2003-07-03 | 2008-09-03 | 中国人民解放军第三○四医院 | 选择性去细胞猪皮在制备人体皮肤代替物中的应用及其制备方法 |
CN1522766A (zh) * | 2003-09-05 | 2004-08-25 | 东南大学 | 脱细胞真皮基质的制备方法 |
KR101107022B1 (ko) * | 2009-09-11 | 2012-01-25 | 한림대학교 산학협력단 | 동결보존 무세포 진피 기질의 제조 방법 및 그로부터 제조된 동결보존 무세포 진피 기질 |
CN103054628A (zh) * | 2013-01-30 | 2013-04-24 | 朱家源 | 一种快速组织工程皮肤构建修复皮肤缺损的方法 |
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