CN103948722B - Chinese medicine composition of preventing and treating heat stress of the animals and preparation method thereof - Google Patents
Chinese medicine composition of preventing and treating heat stress of the animals and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of Chinese medicine composition preventing and treating heat stress of the animals and preparation method thereof, belong to the treatment by Chinese herbs field of heat stress of the animals.First the present invention discloses a kind of Chinese medicine composition preventing and treating heat stress of the animals, including following components: Herba Hedyotidis Diffusae, Herba Moslae, Borneolum Syntheticum, Pericarpium Citri Reticulatae, Fructus Crataegi and Radix Glycyrrhizae.The invention also discloses a kind of method preparing Chinese medicine composition, including: extract Herba Moslae and the volatile oil in Pericarpium Citri Reticulatae and prepare into volatile oil soluble powder；Herba Hedyotidis Diffusae, Fructus Crataegi and Radix Glycyrrhizae and medicinal residues are carried out water carry prepare water promote extract powder；Volatile oil soluble powder, water are promoted extract powder, Borneolum Syntheticum and soluble carrier mix homogeneously.Chinese medicine composition of the present invention has clearing away summer-heat, removing dampness is amusing, effect helped digestion of regulating the flow of vital energy.Animal experiment confirms, has significant curative effect for heat stress, can effectively alleviate sow body temperature and raise, reduce Respiration Rate, slow down hot panting, improve sow daily ingestion amount, reduces piglet mortality rate and improve piglet adding weight.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition, particularly relate to a kind of Chinese medicine composition preventing and treating heat stress of the animals and preparation method thereof, belong to the treatment by Chinese herbs field of heat stress of the animals.
Background technology
Summer high temperature, high humidity weather easily bring out the heat stress of animal.Pig sweat gland is undeveloped, when there is heat stress, transfers peripheral blood circulation, the breathing of strengthening lung with the excretory function of kidney to dispel the heat.Heat radiation is obstructed, and affects blood and blood vessel physiology, easily causes the damage of histoorgan, jeopardize health, carrys out massive losses to industrial belt of raising pigs.
2010 version " People's Republic of China's veterinary drug allusion quotation " two disclose the prescription that veterinary drug clearing away summer-heat dissipates, this prescription is to be mixed after pulverizing by Herba Moslae, Semen Lablab Album, Radix Ophiopogonis, Herba Menthae, Caulis Akebiae, Fructus Gleditsiae Abnormalis, Herba Pogostemonis, Herba Artemisiae Scopariae, Flos Chrysanthemi, Rhizoma Acori Graminei, Flos Lonicerae, Poria, Radix Glycyrrhizae, domestic animal is had effect of clearing summer heat, but prescription taste of Chinese medicine is more, effect same medicine multi-flavor superposition, by only passing through discriminating item its quality uncontrollable in the examination criteria clinic in pharmacopeia.Meanwhile, the dosage form in former prescription is powder, and the consumption of powder is big, and effective ingredient can not well be absorbed, and wants to get a desired effect, it is necessary to strengthen drug dose.But Summer High-Temperature Times, animal is in heat stress state, food ration degradation, and powder adds interpolation in feedstuff, and it is less that drug waste seriously causes medicine to be taken in, and should not play effect.
Chinese medicine extract and preparation thereof can keep traditional administration features of herbal medicine, can extract and retain again the effective ingredient of prescription Chinese medicine to greatest extent, play the Comprehensive Treatment effect of effective ingredient.Extract being made soluble powder, can increase the stability of medicine, convenient transport, simultaneously when animal ingestion amount reduces, drinking-water adds, and can reduce drug waste, give full play to Chinese Herbs.
Summary of the invention
An object of the present invention is to provide the Chinese medicine composition of a kind of effective preventing and treating heat stress of the animals, more to overcome the Chinese medicine composition of existing preventing and treating heat stress of the animals to there are flavour of a drug, it is impossible to control its quality, the defects such as drug dose consumption is big.
The two of the purpose of the present invention are to provide a kind of method preparing above-mentioned Chinese medicine composition.
The above-mentioned purpose of the present invention is achieved through the following technical solutions:
A kind of Chinese medicine composition preventing and treating heat stress of the animals, including following component: Herba Hedyotidis Diffusae, Herba Moslae, Borneolum Syntheticum, Pericarpium Citri Reticulatae, Fructus Crataegi, Radix Glycyrrhizae.
Preferably, the weight portion of each component is: Herba Hedyotidis Diffusae 60-100 part, Herba Moslae 30-60 part, Borneolum Syntheticum 4-6 part, Pericarpium Citri Reticulatae 25-50 part, Fructus Crataegi 40-80 part, Radix Glycyrrhizae 30-60 part.
Most preferred, the weight portion of each component is: Herba Hedyotidis Diffusae 67 parts, Herba Moslae 35 parts, Borneolum Syntheticum 4.2 parts, Pericarpium Citri Reticulatae 35 parts, Fructus Crataegi 45 parts, 35 parts of Radix Glycyrrhizae.
Herba Hedyotidis Diffusae: for the whole herb with root of Maguireothamnus speciosus Herba Hedyotidis Diffusae.Property bitter but sweet flavor, cold.Enter the heart, liver, spleen three warp.Function cures mainly: heat clearing away, dampness removing, removing toxic substances.
Herba Moslae: for the wounded in the battle herb of labiate elsholtzia splendens.Nature and flavor are pungent, tepor.Enter lung, stomach warp.Function cures mainly: diaphoresis expelling summer-heat, row water wet dissipation, during stomach warming is adjusted.
Borneolum Syntheticum: for the processed goods of Spore density Borneolum Syntheticum resin, or it is processed into product for what Camphora, Lignum Pini Nodi wet goods chemically synthesized.Nature and flavor are worked hard, cold.Enter the heart, lung meridian.Function cures mainly: logical all keys, dissipates stagnated fire, removing nebula improving eyesight, reducing swelling and alleviating pain.
Pericarpium Citri tangerinae: different name Pericarpium Citri Reticulatae, for the peel of the multiple Fructus Citri tangerinae classes such as rutaceae tangerine produced in fujian province or Zhu's Fructus Citri tangerinae.Nature and flavor are worked hard, temperature.Enter spleen, lung meridian.Function cures mainly: regulate the flow of vital energy, in tune, and dampness, reduce phlegm.
Fructus Crataegi: for the fruit of rosaceous plant Fructus Crataegi or Crataegi cuneatae.Nature and flavor acid is sweet, tepor.Enter spleen, stomach, Liver Channel.Function cures mainly: removing food stagnancy is eaten, dissipating blood stasis blood, expelling tenia.
Radix Glycyrrhizae: for root and the root stock of glycyrrhizic legume.Nature and flavor are sweet, flat.Enter spleen, stomach, lung meridian.Function cures mainly: with middle emergency, lung moistening, removing toxic substances, coordinating the actions of various ingredients in a prescription.
When drug composition adds the crude drug of following weight portion in the present invention, there is better wind-dispelling heat-dissipating, ward off effect of dirty damp eliminating: Radix Ophiopogonis 25-40 part and Herba Menthae 30-45 part；Preferably, the weight portion of each crude drug is: Radix Ophiopogonis 30 parts and Herba Menthae 25 parts.
Radix Ophiopogonis: different name Radix Ophiopogonis, for the tuber of liliaceous plant dwarf lilyturf.Property sweet and slightly bitter taste, cold.Enter lung, stomach, heart channel.Function cures mainly: nourishing YIN and moistening the lung, clear away heart-fire relieving restlessness, reinforcing stomach reg fluid.
Herba Menthae: for herb or the leaf of labiate Herba Menthae or family Herba Menthae.Nature and flavor are pungent, cool.Enter lung, Liver Channel.Function cures mainly: dispelling wind, heat radiation, wards off dirty, removing toxic substances.
Heat stress belongs to heatstroke category in the traditional Chinese medical science, and summer, the gas ratio of heat-damp in summer was more serious, and pathogenic summer-heat belongs to heat and belongs to fire, very easily disturbs the mind, and consumes impairment of QI Tianjin.The taste of damp puzzlement simultaneously, affect animal appetite, and then affect the physiological function of animal and produce performance.Therefore want relieving the exterior syndrome by diaphoresis from its Therapeutic Principle, rush down lower heat extraction, clearing away heat-damp and promoting diuresis, regulating the flow of vital energy helps digestion.Prescription of the present invention is by the classical prescription dissipated in conjunction with clearing away summer-heat and above theory, and according to heat stress feature, based on heat clearing away of driving away summer heat, addition removing dampness is amusing, the composition helped digestion of regulating the flow of vital energy.
Prescription of the present invention is made up of Herba Hedyotidis Diffusae, Herba Moslae, Radix Ophiopogonis, Herba Menthae, Borneolum Syntheticum, Pericarpium Citri Reticulatae, Fructus Crataegi and Radix Glycyrrhizae.Herba Hedyotidis Diffusae in side: heat-clearing and toxic substances removing, medicinal powder for relieving pain knot, promoting urination to remove dampness, for monarch drug；Herba Moslae energy diaphoresis expelling summer-heat, and middle dampness removing, promoting qi circulation, resolving food retention, can be effectively improved the card of the heat-damp in summer that heat stress causes, Herba Menthae dispelling wind and heat pathogens, and the clear profit head, is ministerial drug altogether；Borneolum Syntheticum: refreshment of having one's ideas straightened out, heat clearing away dissipates poison, and Radix Ophiopogonis: clearing away heart-fire and moistening the lung, reinforcing stomach reg fluid, Pericarpium Citri Reticulatae: regulating qi-flowing for strengthening spleen, drying dampness to eliminate phlegm, Fructus Crataegi: promoting digestion and removing stagnation, circulation of qi promoting dissipating blood stasis, for adjuvant drug；Radix Glycyrrhizae: spleen reinforcing stomach function regulating, coordinating the actions of various ingredients in a prescription.All medicines share, and play clearing away summer-heat altogether, removing dampness is amusing, effect helped digestion of regulating the flow of vital energy, to alleviate the high temperature harmful effect to pig.
Each taste crude drug used by the present invention can be commercially available at pharmacy.
Adopt the customary preparation methods of Chinese medicine preparation, it is possible to Chinese medicine composition of the present invention is prepared into any preparation being suitable to animal oral administration, for instance can be powder, oral liquid, powder etc.；It is preferably and prepares into soluble powder, can be used for spice and drinking water administration in clinic, to reduce its Clinical practice amount, easy to use, improve palatability.
The invention provides a kind of method preparing described Chinese medicine composition, comprise the following steps:
(1) Herba Moslae and Pericarpium Citri Reticulatae pulverizing steam distillation being extracted volatile oil, volatile oil is standby；Extracting solution filters, and filtrate and medicinal residues are standby respectively；
(2) extract the medicinal residues after volatile oil and together carry out water with step (1) and carry after Herba Hedyotidis Diffusae, Fructus Crataegi and Radix Glycyrrhizae being pulverized, Aqueous extracts filters, filtrate reduced in volume to relative density is the extractum of 1.05-1.35, adding ethanol makes alcohol content reach 60%, stands, and filters, filtrate reduced in volume, reclaim ethanol, obtain water extracting liquid, standby；
(3) in the volatile oil extracted, add the antitackiness agent accounting for volatile oil gross mass 0.5-1.5%, add amount of solid and account for the filler of volatile oil gross mass 75-250%, stirring, high pressure homogenize, spray drying, obtain volatile oil soluble powder；
(4) in water extracting liquid, addition accounts for water extracting liquid gross mass 10-30% filler and accounts for the antitackiness agent of water extracting liquid gross mass 0.2-1%, and spray drying obtains water and promotes extract powder；
(5) volatile oil soluble powder, water are promoted extract powder, finely divided ice and soluble carrier mix homogeneously, to obtain final product.
Present invention also offers a kind of method preparing Chinese medicine composition described in claim 1, comprise the following steps:
(1) Herba Moslae, Herba Menthae and Pericarpium Citri Reticulatae pulverizing steam distillation being extracted volatile oil, volatile oil is standby；Extracting solution filters, and filtrate and medicinal residues are standby respectively；
(2) extract the medicinal residues after volatile oil and together carry out water with step (1) and carry after Herba Hedyotidis Diffusae, Radix Ophiopogonis, Fructus Crataegi and Radix Glycyrrhizae being pulverized, Aqueous extracts filters, filtrate reduced in volume to relative density is the extractum of 1.05-1.35, adding ethanol makes alcohol content reach 60%, stands, and filters, filtrate reduced in volume, reclaim ethanol, obtain water extracting liquid, standby；
(3) in the volatile oil extracted, add the antitackiness agent accounting for volatile oil gross mass 0.5-1.5%, add amount of solid and account for the filler of volatile oil gross mass 75-250%, stirring, high pressure homogenize, spray drying, obtain volatile oil soluble powder；
(4) in water extracting liquid, addition accounts for water extracting liquid gross mass 10-30% filler and accounts for the antitackiness agent of water extracting liquid gross mass 0.2-1%, and spray drying obtains water and promotes extract powder；
(5) volatile oil soluble powder, water are promoted extract powder, finely divided ice and soluble carrier mix homogeneously, to obtain final product.
All containing volatile oil in Herba Moslae, Herba Menthae, Pericarpium Citri Reticulatae, be not only volatile oil, their water soluble ingredient also should be the main portions of its performance curative effect.In order to extract to greatest extent and retain the effective ingredient of each medicine in prescription, playing the Comprehensive Treatment effect of effective ingredient, it is ensured that clinical efficacy, after Herba Moslae, Herba Pogostemonis, Herba Menthae, Pericarpium Citri Reticulatae are extracted volatile oil, medicinal residues together add and carry out water and carry.
The present invention adopts steam distillation to extract the volatile oil composition in Herba Moslae, Herba Menthae, Pericarpium Citri Reticulatae.In order to improve volatile oil is drawn effect, shortening and carry the oil time, medical material adds 10 times amount water soaking 1～2 hour so that it is start distillation extraction after being thoroughly impregnated with.The volatile oil is drawn time is too short, extracts not exclusively；Overlong time, energy consumption is big, man-hour length, production cost high, in order to find out optimum extraction time, present aspect has carried out screening test, volatile oil is collected with volatile oil determination apparatus, and within the time such as 3 hours, 4 hours, 5 hours, 6 hours, 7 hours, 8 hours, read volatile oil volume, carry out three repeated trials, obtain the meansigma methods of volatile oil is drawn amount；Screening test result shows: extraction time is bigger on volatile oil is drawn impact, extracting the critical point 6 hours being to extract, at 6 hours these time points, in each medical material, volatile oil extracted completely substantially, even if being further added by extraction time, the yield of volatile oil is substantially free of growth.Therefore, the present invention when extracting the volatile oil composition in Herba Moslae, Herba Menthae, Pericarpium Citri Reticulatae with steam distillation；In medical material, preferably add 10 times of quality water soakings after 2 hours, distillation extraction 6 hours, to greatest extent the volatile oil component in medical material can not only be extracted, and can effectively reduce production cost, improve efficiency.
The volatilization oil mass that the present invention extracts from Herba Moslae, Herba Menthae, Pericarpium Citri Reticulatae is higher, and volatile oil is one of important activity component in present aspect prescription, if directly volatile oil being added in preparation, volatile poorly water-soluble and not easily granulating, cause that preparation is unstable and not easily takes.Volatile oil is carried out emulsifying by the present invention and inclusion processes, and makes volatile oil powdered, in order to make solid preparation, it is possible to decrease its volatility, increases water solublity, thus improving stability and the bioavailability of preparation.The present invention is found by experiment that, the kind of emulsifying agent and consumption have the impact of highly significant for emulsifying effectiveness.The present invention is found by primary screening test, compares the emulsifying agent of other kind, and composite for Tween-80 and the Span-80 compound emulsifying agent together obtained has the emulsifying effectiveness of the best；In order to screen consumption and Tween-80 and the Span-80 (Span-80 of suitable emulsifying agent further；Sorbitol anhydride oleate；S-80 emulsifying agent) proportion compatibility, the present invention carrys out the proportion compatibility of optimized choice emulsifier and Tween-80 and Span-80 by observing oil-water separation phenomenon, when Tween-80:Span-80 (mass ratio) is 2:1, affiliated total amount has the emulsifying effectiveness of the best when being volatile oil total amount 20%: obtained emulsion outward appearance is homogeneous milky, centrifugal test finds, this emulsion is highly stable, it is absent from lamination, but the emulsifying agent of other proportioning all exists comparatively serious lamination.
By adjusting suitable emulsifier and ratio, select suitable filler, after obtaining the volatile oil emulsion of stable homogeneous, mechanism again through spray dryer, emulsion is dispersed into the very thin microgranule (increasing moisture disengagement area, accelerate dry run) as mist and hot air, in moment, most of moisture is removed, make volatile oil emulsion dry, obtain water solublity volatilization oil meal.
Principle active component Radix Ophiopogonis soap Radix Ophiopogonis former times, polysaccharide, flavone etc., Herba Hedyotidis Diffusae contains Anthraquinones, flavonoid, organic acid, polysaccharide etc., Fructus Crataegi contains flavone and polysaccharide, Radix Glycyrrhizae mainly contains glycyrrhizic acid, flavonoid, polysaccharide composition, therefore Radix Ophiopogonis, Herba Hedyotidis Diffusae, Fructus Crataegi, Radix Glycyrrhizae is extracted for solvent with water.
Described antitackiness agent include but not limited in Pulvis Talci, micropowder silica gel, magnesium stearate or Magnesiumaluminumsilicate any one or multiple；
Described filler includes but not limited to one or more mixing in soluble starch, maltodextrin, lactose, mannitol, glucose, sorbitol or sucrose etc.；
Described soluble carrier includes but not limited to soluble starch, maltodextrin, lactose, mannitol, glucose, sorbitol or sucrose.
Described filler includes but not limited to one or more in arabic gum, corn syrup, maltodextrin, chitosan etc.；
Results of animal finds, Chinese medicine composition of the present invention can significantly improve the body temperature under nursing sow heat stress state and respiratory frequency, alleviates sow body temperature and raises, reduces Respiration Rate, slow down hot panting, be effectively improved its physiological status；Sow feed intake can be obviously improved, improve sow daily ingestion amount, reduce piglet mortality rate, improve piglet average daily gain amount.In addition, after the Chinese medicine composition of the present invention is prepared into soluble powder, effect for improving piglet daily gain to be substantially better than powder group and the curative effect of veterinary drug clearing away summer-heat scattered (veterinary drug allusion quotation prescription), it was shown that adopts the soluble powder prepared by preparation method of present aspect can effectively improve therapeutic effect.
The usage of Chinese medicine composition of the present invention and consumption: soluble powder, 12-20g/ head pig, spice or add water is drunk.
Detailed description of the invention
The present invention is further described below in conjunction with specific embodiment; these embodiments are only exemplary; what those skilled in the art should understand that is; the details of technical solution of the present invention and form can be modified or replace lower without departing from the spirit and scope of the present invention, but these amendments and replacement each fall within protection scope of the present invention.
The preparation of embodiment 1 soluble powder
Each crude drug (unit: kg) is weighed: Herba Hedyotidis Diffusae 60, Herba Moslae 30, Borneolum Syntheticum 4, Pericarpium Citri Reticulatae 25, Fructus Crataegi 40, Radix Glycyrrhizae 30 by following weight.
Adjuvant: Tween-80, Span-80, aluminosilicate magnesium, maltodextrin, arabic gum, soluble starch, Pulvis Talci, wherein: Tween-80, Span-80 are emulsifying agent；Aluminosilicate magnesium, Pulvis Talci are antitackiness agent；Maltodextrin, arabic gum, soluble starch are filler and carrier.
Preparation method:
(1) volatile oil is drawn
Herba Moslae, Pericarpium Citri Reticulatae coarse crushing being become 20 order sizes, puts in 3 tons of multi-function extractors, add water 10 times amount, soaks 2h, extracts 6h, collects volatile oil 1.6kg, and extracting solution filters, and filtrate and medicinal residues are standby respectively.
(2) extraction process by water
Herba Hedyotidis Diffusae, Fructus Crataegi and Radix Glycyrrhizae coarse crushing are become 20 order sizes, extract the medicinal residues after volatile oil together with Herba Moslae, Pericarpium Citri Reticulatae together to put into and 3 tons of multi-function extractors carry out water carry, extract 3 times, first time adds the filtrate after volatile oil is drawn and purified water totally 10 times amount, extracting 2h, second and third time is separately added into 10,8 times amount purified water and respectively extracts 2h, 1h.Filtrate squeezes into 300 mesh screen through pump, merging filtrate, it is evaporated to the extractum that relative density is 1.05-1.35 (50 DEG C), adds ethanol and make alcohol content reach 60%, stand, 300 orders filter, filtrate concentrates through vacuum-concentrcted device, evaporating temperature: 50-75 DEG C, reclaims ethanol, being concentrated into every 1ml medicinal liquid and be equivalent to 1g medical material, concentrated solution is standby.
(3) preparation of volatile oil soluble powder
Volatile oil 1.6kg step (1) extracted adds Tween-80213.3g, Span-80106.7g, aluminosilicate magnesium 15g, after mix homogeneously, limit is quickly stirred limit and is added 20% arabic gum 6.4L, 30% soluble starch 8L, in high speed homogenizer after 16000rpm high shear agitation 30min, emulsifying 30min in colloid mill (emulsifying fineness >=2um) again, obtain relatively stable homogeneous emulsion, finally carry out spray drying, drying process with atomizing condition is: feeding temperature 50～60 DEG C, feed rate 150ml/min, inlet temperature 185～195 DEG C, leaving air temp 65～70 DEG C.
(4) the water extracted immersing paste spray drying
The water extracting liquid (280L) of concentration adds 6kg soluble starch, 1.4kg Pulvis Talci (300 order), fully dissolves and stir evenly, by spray dryer, water extracting liquid being spray dried to dry extract.Drying process with atomizing condition is: feeding temperature 50～60 DEG C, feed rate 150ml/min, inlet temperature 185～195 DEG C, leaving air temp 65～70 DEG C.
(5) powder body mixing
Volatile oil soluble powder 5.5kg, water promote extract powder 55kg, Borneolum Syntheticum 4.2kg, maltodextrin 5.3kg, altogether 70kg.First by after Borneolum Syntheticum and a small amount of soluble starch premix, then all powder is fully mixed together, to obtain final product.
The preparation of embodiment 2 soluble powder
Each crude drug (unit: kg) is weighed: Herba Hedyotidis Diffusae 100, Herba Moslae 60, Borneolum Syntheticum 6, Pericarpium Citri Reticulatae 50, Fructus Crataegi 80, Radix Glycyrrhizae 60 by following weight.
Adjuvant: Tween-80, Span-80, aluminosilicate magnesium, maltodextrin, arabic gum, soluble starch, Pulvis Talci, wherein: Tween-80, Span-80 are emulsifying agent；Aluminosilicate magnesium, Pulvis Talci are antitackiness agent；Maltodextrin, arabic gum, soluble starch are filler and carrier.
Preparation method:
(1) volatile oil is drawn
Herba Moslae, Pericarpium Citri Reticulatae coarse crushing being become 20 order sizes, puts in 3 tons of multi-function extractors, add water 10 times amount, soaks 2h, extracts 6h, collects volatile oil 1.6kg, and extracting solution filters, and filtrate and medicinal residues are standby respectively.
(2) extraction process by water
Herba Hedyotidis Diffusae, Fructus Crataegi and Radix Glycyrrhizae coarse crushing are become 20 order sizes, extract the medicinal residues after volatile oil together with Herba Moslae, Pericarpium Citri Reticulatae together to put into and 3 tons of multi-function extractors carry out water carry, extract 3 times, first time adds the filtrate after volatile oil is drawn and purified water totally 10 times amount, extracting 2h, second and third time is separately added into 10,8 times amount purified water and respectively extracts 2h, 1h.Filtrate squeezes into 300 mesh screen through pump, merging filtrate, it is evaporated to the extractum that relative density is 1.05-1.35 (50 DEG C), adds ethanol and make alcohol content reach 60%, stand, 300 orders filter, filtrate concentrates through vacuum-concentrcted device, evaporating temperature: 50-75 DEG C, reclaims ethanol, being concentrated into every 1ml medicinal liquid and be equivalent to 1g medical material, concentrated solution is standby.
(3) preparation of volatile oil soluble powder
Volatile oil 1.6kg step (1) extracted adds Tween-80213.3g, Span-80106.7g, aluminosilicate magnesium 15g, after mix homogeneously, limit is quickly stirred limit and is added 20% arabic gum 6.4L, 30% soluble starch 8L, in high speed homogenizer after 16000rpm high shear agitation 30min, emulsifying 30min in JM50C colloid mill (emulsifying fineness >=2um) again, obtain relatively stable homogeneous emulsion, finally carry out spray drying, drying process with atomizing condition is: feeding temperature 50～60 DEG C, feed rate 150ml/min, inlet temperature 185～195 DEG C, leaving air temp 65～70 DEG C.
(4) the water extracted immersing paste spray drying
The water extracting liquid (280L) of concentration adds 6kg soluble starch, 1.4kg Pulvis Talci (300 order), fully dissolves and stir evenly, by spray dryer, water extracting liquid being spray dried to dry extract.Drying process with atomizing condition is: feeding temperature 50～60 DEG C, feed rate 150ml/min, inlet temperature 185～195 DEG C, leaving air temp 65～70 DEG C.
(5) powder body mixing
Volatile oil soluble powder 5.5kg, water promote extract powder 55kg, Borneolum Syntheticum 4.2kg, maltodextrin 5.3kg, altogether 70kg.First by after Borneolum Syntheticum and a small amount of soluble starch premix, then all powder is fully mixed together, to obtain final product.
The preparation of embodiment 3 soluble powder
Each crude drug (unit: kg) is weighed: Herba Hedyotidis Diffusae 67, Herba Moslae 35, Borneolum Syntheticum 4.2, Pericarpium Citri Reticulatae 35, Fructus Crataegi 45, Radix Glycyrrhizae 35 by following weight；
Adjuvant: Tween-80, Span-80, aluminosilicate magnesium, maltodextrin, arabic gum, soluble starch, Pulvis Talci, wherein: Tween-80, Span-80 are emulsifying agent；Aluminosilicate magnesium, Pulvis Talci are antitackiness agent；Maltodextrin, arabic gum, soluble starch are filler and carrier.
Preparation method:
(1) volatile oil is drawn
Herba Moslae, Pericarpium Citri Reticulatae coarse crushing being become 20 order sizes, puts in 3 tons of multi-function extractors, add water 10 times amount, soaks 2h, extracts 6h, collects volatile oil 1.6kg, and extracting solution filters, and filtrate and medicinal residues are standby respectively.
(2) extraction process by water
Herba Hedyotidis Diffusae, Fructus Crataegi and Radix Glycyrrhizae coarse crushing are become 20 order sizes, extract the medicinal residues after volatile oil together with Herba Moslae, Pericarpium Citri Reticulatae together to put into and 3 tons of multi-function extractors carry out water carry, extract 3 times, first time adds the filtrate after volatile oil is drawn and purified water totally 10 times amount, extracting 2h, second and third time is separately added into 10,8 times amount purified water and respectively extracts 2h, 1h.Filtrate squeezes into 300 mesh screen through pump, merging filtrate, it is evaporated to the extractum that relative density is 1.05-1.35 (50 DEG C), adds ethanol and make alcohol content reach 60%, stand, 300 orders filter, filtrate concentrates through vacuum-concentrcted device, evaporating temperature: 50-75 DEG C, reclaims ethanol, being concentrated into every 1ml medicinal liquid and be equivalent to 1g medical material, concentrated solution is standby.
(3) preparation of volatile oil soluble powder
Volatile oil 1.6kg step (1) extracted adds Tween-80213.3g, Span-80106.7g, aluminosilicate magnesium 15g, after mix homogeneously, limit is quickly stirred limit and is added 20% arabic gum 6.4L, 30% soluble starch 8L, in high speed homogenizer after 16000rpm high shear agitation 30min, emulsifying 30min in JM50C colloid mill (emulsifying fineness >=2um) again, obtain relatively stable homogeneous emulsion, finally carry out spray drying, drying process with atomizing condition is: feeding temperature 50～60 DEG C, feed rate 150ml/min, inlet temperature 185～195 DEG C, leaving air temp 65～70 DEG C.
(4) the water extracted immersing paste spray drying
The water extracting liquid (280L) of concentration adds 6kg soluble starch, 1.4kg Pulvis Talci (300 order), fully dissolves and stir evenly, by spray dryer, water extracting liquid being spray dried to dry extract.Drying process with atomizing condition is: feeding temperature 50～60 DEG C, feed rate 150ml/min, inlet temperature 185～195 DEG C, leaving air temp 65～70 DEG C.
(5) powder body mixing
Volatile oil soluble powder 5.5kg, water promote extract powder 55kg, Borneolum Syntheticum 4.2kg, maltodextrin 5.3kg, altogether 70kg.First by after Borneolum Syntheticum and a small amount of soluble starch premix, then all powder is fully mixed together, to obtain final product.
The preparation of embodiment 4 soluble powder
Each crude drug (unit: kg) is weighed: Herba Hedyotidis Diffusae 60, Herba Moslae 30, Borneolum Syntheticum 4, Pericarpium Citri Reticulatae 25, Fructus Crataegi 40, Radix Glycyrrhizae 30, Radix Ophiopogonis 25, Herba Menthae 30 by following weight.
Adjuvant: Tween-80, Span-80, aluminosilicate magnesium, maltodextrin, arabic gum, soluble starch, Pulvis Talci, wherein: Tween-80, Span-80 are emulsifying agent；Aluminosilicate magnesium, Pulvis Talci are antitackiness agent；Maltodextrin, arabic gum, soluble starch are filler and carrier.
Preparation method:
(1) volatile oil is drawn
Herba Moslae, Herba Pogostemonis, Herba Menthae, Pericarpium Citri Reticulatae coarse crushing being become 20 order sizes, puts in 3 tons of multi-function extractors, add water 10 times amount, soaks 2h, extracts 6h, collects volatile oil 1.6kg, and extracting solution filters, and filtrate and medicinal residues are standby respectively.
(2) extraction process by water
Radix Ophiopogonis, Herba Hedyotidis Diffusae, Fructus Crataegi and Radix Glycyrrhizae coarse crushing are become 20 order sizes, extract the medicinal residues after volatile oil together with Herba Moslae, Herba Pogostemonis, Herba Menthae, Pericarpium Citri Reticulatae together to put into and 3 tons of multi-function extractors carry out water carry, extract 3 times, first time adds the filtrate after volatile oil is drawn and purified water totally 10 times amount, extracting 2h, second and third time is separately added into 10,8 times amount purified water and respectively extracts 2h, 1h.Filtrate squeezes into 300 mesh screen through pump, merging filtrate, it is evaporated to the extractum that relative density is 1.05-1.35 (50 DEG C), adds ethanol and make alcohol content reach 60%, stand, 300 orders filter, filtrate concentrates through vacuum-concentrcted device, evaporating temperature: 50-75 DEG C, reclaims ethanol, being concentrated into every 1ml medicinal liquid and be equivalent to 1g medical material, concentrated solution is standby.
(3) preparation of volatile oil soluble powder
Volatile oil 1.6kg step (1) extracted adds Tween-80213.3g, Span-80106.7g, aluminosilicate magnesium 15g, after mix homogeneously, limit is quickly stirred limit and is added 20% arabic gum 6.4L, 30% soluble starch 8L, in high speed homogenizer after 16000rpm high shear agitation 30min, emulsifying 30min in JM50C colloid mill (emulsifying fineness >=2um) again, obtain relatively stable homogeneous emulsion, finally carry out spray drying, drying process with atomizing condition is: feeding temperature 50～60 DEG C, feed rate 150ml/min, inlet temperature 185～195 DEG C, leaving air temp 65～70 DEG C.
(4) the water extracted immersing paste spray drying
The water extracting liquid (280L) of concentration adds 6kg soluble starch, 1.4kg Pulvis Talci (300 order), fully dissolves and stir evenly, by spray dryer, water extracting liquid being spray dried to dry extract.Drying process with atomizing condition is: feeding temperature 50～60 DEG C, feed rate 150ml/min, inlet temperature 185～195 DEG C, leaving air temp 65～70 DEG C.
(5) powder body mixing
Volatile oil soluble powder 5.5kg, water promote extract powder 55kg, Borneolum Syntheticum 4.2kg, maltodextrin 5.3kg, altogether 70kg.First by after Borneolum Syntheticum and a small amount of soluble starch premix, then all powder is fully mixed together, to obtain final product.
The preparation of embodiment 5 soluble powder
Each crude drug (unit: kg) is weighed: Herba Hedyotidis Diffusae 100, Herba Moslae 60, Borneolum Syntheticum 6, Pericarpium Citri Reticulatae 50, Fructus Crataegi 80, Radix Glycyrrhizae 60, Radix Ophiopogonis 40, Herba Menthae 45 by following weight；
Adjuvant: Tween-80, Span-80, magnesium stearate, maltodextrin, arabic gum, soluble starch, Pulvis Talci, wherein: Tween-80, Span-80 are emulsifying agent, magnesium stearate, Pulvis Talci are antitackiness agent, maltodextrin, arabic gum, soluble starch is filler and carrier.
Preparation method:
(1) volatile oil is drawn
Herba Moslae, Herba Pogostemonis, Pericarpium Citri Reticulatae coarse crushing are become 20 order sizes, puts in 200L multi-function extractor, amount of water 10 times amount, soak 2h, extract 6h, collect volatile oil 95.2g, extracting solution filters, and filtrate and medicinal residues are standby respectively.
(2) extraction process by water
Radix Ophiopogonis, Herba Hedyotidis Diffusae, Fructus Crataegi, Radix Glycyrrhizae coarse crushing are become 20 order sizes, extract medicinal residues after volatile oil together with Herba Moslae, Herba Menthae, Pericarpium Citri Reticulatae together to put into and 200L multi-function extractor carries out water carry, extract 3 times, first time adds the filtrate after volatile oil is drawn and purified water totally 12 times amount, extracting 2h, second and third time is separately added into 10,8 times amount purified water and respectively extracts 1.5 hours.Filtrate squeezes into 300 mesh screen through pump, merging filtrate, it is evaporated to the extractum that relative density is 1.05-1.35 (50 DEG C), adds ethanol and make alcohol content reach 60%, stand, 300 orders filter, filtrate concentrates through vacuum-concentrcted device, evaporating temperature: 50-75 DEG C, reclaims ethanol, being concentrated into every 1ml medicinal liquid and be equivalent to 1g medical material, concentrated solution is standby.
(3) preparation of volatile oil soluble powder
The volatile oil 95.2g that step (1) is extracted, Tween-8012.7g, Span-806.5g, magnesium stearate 1g, after mix homogeneously, limit is quickly stirred limit and is added 20% arabic gum 400ml, 30% maltodextrin 500ml, in high speed homogenizer after 16000rpm high shear agitation 30min, emulsifying 30min in JM50C colloid mill (emulsifying fineness >=2um) again, obtain relatively stable homogeneous emulsion, finally carry out spray drying, drying process with atomizing condition: drying process with atomizing condition is: feeding temperature 50～60 DEG C, feed rate 150ml/min, inlet temperature 185～195 DEG C, leaving air temp 65～70 DEG C.
(4) the water extracted immersing paste spray drying
The water extracting liquid (16L) of concentration adds 400g soluble starch, 80g Pulvis Talci (300 order), fills dissolving and stir evenly, by spray dryer, water extracting liquid being spray dried to dry extract.Drying process with atomizing condition is: feeding temperature 50～60 DEG C, feed rate 150ml/min, inlet temperature 185～195 DEG C, leaving air temp 65～70 DEG C.
(5) powder body mixing
Volatile oil soluble powder 0.34kg, water promote extract powder 3.35kg, Borneolum Syntheticum 0.2kg, soluble starch 0.11kg, altogether after 4kg, Borneolum Syntheticum and a small amount of soluble starch premix, are more fully mixed together by all powder, to obtain final product.
The preparation of embodiment 6 soluble powder
Each crude drug (unit: kg) is weighed: Herba Hedyotidis Diffusae 67, Herba Moslae 35, Borneolum Syntheticum 4.2, Pericarpium Citri Reticulatae 35, Fructus Crataegi 45, Radix Glycyrrhizae 35, Radix Ophiopogonis 30, Herba Menthae 35 by following weight；
Adjuvant: Tween-80, Span-80, aluminosilicate magnesium, maltodextrin, arabic gum, soluble starch, Pulvis Talci, wherein: Tween-80, Span-80 are emulsifying agent；Aluminosilicate magnesium, Pulvis Talci are antitackiness agent；Maltodextrin, arabic gum, soluble starch are filler and carrier.
Preparation method:
(1) volatile oil is drawn
Herba Moslae, Herba Pogostemonis Pericarpium Citri Reticulatae coarse crushing being become 20 order sizes, puts in 3 tons of multi-function extractors, add water 10 times amount, soaks 2h, extracts 6h, collects volatile oil 1.6kg, and extracting solution filters, and filtrate and medicinal residues are standby respectively.
(2) extraction process by water
Radix Ophiopogonis, Herba Hedyotidis Diffusae, Fructus Crataegi and Radix Glycyrrhizae coarse crushing are become 20 order sizes, extract the medicinal residues after volatile oil together with Herba Moslae, Herba Menthae, Pericarpium Citri Reticulatae together to put into and 3 tons of multi-function extractors carry out water carry, extract 3 times, first time adds the filtrate after volatile oil is drawn and purified water totally 10 times amount, extracting 2h, second and third time is separately added into 10,8 times amount purified water and respectively extracts 2h, 1h.Filtrate squeezes into 300 mesh screen through pump, merging filtrate, it is evaporated to the extractum that relative density is 1.05-1.35 (50 DEG C), adds ethanol and make alcohol content reach 60%, stand, 300 orders filter, filtrate concentrates through vacuum-concentrcted device, evaporating temperature: 50-75 DEG C, reclaims ethanol, being concentrated into every 1ml medicinal liquid and be equivalent to 1g medical material, concentrated solution is standby.
(3) preparation of volatile oil soluble powder
Volatile oil 1.6kg step (1) extracted adds Tween-80213.3g, Span-80106.7g, aluminosilicate magnesium 15g, after mix homogeneously, limit is quickly stirred limit and is added 20% arabic gum 6.4L, 30% soluble starch 8L, in high speed homogenizer after 16000rpm high shear agitation 30min, emulsifying 30min in JM50C colloid mill (emulsifying fineness >=2um) again, obtain relatively stable homogeneous emulsion, finally carry out spray drying, drying process with atomizing condition is: feeding temperature 50～60 DEG C, feed rate 150ml/min, inlet temperature 185～195 DEG C, leaving air temp 65～70 DEG C.
(4) the water extracted immersing paste spray drying
The water extracting liquid (280L) of concentration adds 6kg soluble starch, 1.4kg Pulvis Talci (300 order), fully dissolves and stir evenly, by spray dryer, water extracting liquid being spray dried to dry extract.Drying process with atomizing condition is: feeding temperature 50～60 DEG C, feed rate 150ml/min, inlet temperature 185～195 DEG C, leaving air temp 65～70 DEG C.
(5) powder body mixing
Volatile oil soluble powder 5.5kg, water promote extract powder 55kg, Borneolum Syntheticum 4.2kg, maltodextrin 5.3kg, altogether 70kg.First by after Borneolum Syntheticum and a small amount of soluble starch premix, then all powder is fully mixed together, to obtain final product.
The optimization experiment of experimental example 1 soluble powder preparation method
Each crude drug (unit: kg) is weighed: Herba Moslae 35, Herba Menthae 35, Pericarpium Citri Reticulatae 35 by following weight；
Adjuvant: Tween-80, Span-80 are emulsifying agent.
1, the determination of volatile oil is drawn time
All containing volatile oil in Herba Moslae, Herba Menthae, Pericarpium Citri Reticulatae, the present invention adopts steam distillation to extract the volatile oil composition in each medical material.In order to improve volatile oil is drawn effect, shortening and carry the oil time, medical material adds 8～10 times of quality water, soaks 1～2h so that it is start distillation extraction after being thoroughly impregnated with.The volatile oil is drawn time is too short, extracts not exclusively；Overlong time, energy consumption is big, man-hour length, production cost high.For this, the volatile oil is drawn time has been tested, has collected volatile oil with volatile oil determination apparatus, and within the time such as 3 hours, 4 hours, 5 hours, 6 hours, 7 hours, 8 hours, read volatile oil volume, carrying out three repeated trials, obtain the meansigma methods of volatile oil is drawn amount, result is in Table 1.
The volatile oil is drawn amount of table 1 different extraction time
Above result of the test shows, extraction time is bigger on the impact of volatile oil is drawn amount；Extract 6h substantially to extract completely.Hereafter, even if being further added by extraction time, the increase of the yield of volatile oil can be ignored substantially.Therefore, present invention determine that the optimum extraction time of volatile oil is 6h, volatile oil yield is 1.45%.2, the selection of emulsifier proportion and consumption
The present invention is found by Preliminary screening screening test, and the emulsifying of volatile oil adopts Tween-80 and Span-80 composite emulsifier, and the emulsifying effectiveness of the emulsifying agent that its emulsifying effectiveness is substantially better than other kind has.Optimum consumption and the optimum proportioning ratio of the two of composite emulsifier is screened in this experiment further by observing oil-water separation phenomenon.
Take the volatile oil extracted from Herba Moslae, Herba Menthae and Pericarpium Citri Reticulatae a small amount of, it is separately added into Tween-80 and the Span-80 (emulsifier proportion and consumption are in Table 2) of a certain amount of different proportion, after stirring, being slowly added to the water of 3 times of quality, limit edged stirs, it is dividedly in some parts, after water injection continuing stirring 15min afterwards, observe the change of emulsion, the person of stablizing is centrifuged test again, 3000r/min, centrifugal 5min.Observing delamination, result of the test is in Table 2.
The experimental result that emulsifying effectiveness is affected by the consumption of the different emulsifiers added during table 2 volatile oil emulsifying and ratio
From table 2, when Tween-80 and Span-80 (mass ratio) be 2:1, affiliated quality for accounting for volatile oil total amount 20% time, obtained emulsion is the most stable, also not stratified after centrifugal, has the emulsifying effectiveness of the best compared with other emulsifying agent.
The effect test of test example 1 Chinese medicine composition preventing and treating nursing sow heat stress of the present invention
1, for reagent thing
For reagent thing (1): clearing away summer-heat dissipates (veterinary drug allusion quotation prescription): Herba Moslae 30g, Semen Lablab Album 30g, Radix Ophiopogonis 25g, Herba Menthae 30g, Caulis Akebiae 25g, Fructus Gleditsiae Abnormalis 20g, Herba Pogostemonis 30g, Herba Artemisiae Scopariae 25g, Flos Chrysanthemi 30g, Rhizoma Acori Graminei 25g, Flos Lonicerae 60g, Poria 25g, Radix Glycyrrhizae 15g；By above-mentioned each component mix homogeneously, pulverize, cross 40 mesh sieves, obtain powder.
For reagent thing (2): Herba Hedyotidis Diffusae 67g, Herba Moslae 35g, Borneolum Syntheticum 4.2g, Pericarpium Citri Reticulatae 35g, Fructus Crataegi 45g, Radix Glycyrrhizae 35g, Radix Ophiopogonis 30g, Herba Menthae 35g；By above-mentioned each component mix homogeneously, pulverize, cross 40 mesh sieves, obtain powder；
For reagent thing (3): Herba Hedyotidis Diffusae 67g, Herba Moslae 35g, Borneolum Syntheticum 4.2g, Pericarpium Citri Reticulatae 35g, Fructus Crataegi 45g, Radix Glycyrrhizae 35g；By above-mentioned each component mix homogeneously, pulverize, cross 40 mesh sieves, obtain powder；
For reagent thing (4): the soluble powder of embodiment 6 preparation；
For reagent thing (5): the soluble powder of embodiment 3 preparation.
2, test method
2.1 experimental animals and packet
Test and carry out on pig farm, ten thousand, Caidian District, Hubei Province on July 20th, 2013 to August 20, select that 2～3 tires, kind be consistent, expected date of confinement on July about 27 female Landrace 30.30 sows are randomly divided into 6 process, each process 5 repetition, 1 sow of each repetition.6 process groups are randomly divided into matched group, test A, B, C, D and E group, test A group is with for reagent thing (1), test B group is with for reagent thing (2), test C group is with for reagent thing (3), test D group is with for reagent thing (4), and test E group is with for reagent thing (5)；
2.2 trial
In experimental period, delivery room temperature is maintained at 30～35 DEG C.Test period is: 21d after 7d extremely childbirth before sow expected date of confinement, feedstuff is that pig farm buys pregnancy, nursing sow and weaning pigs by oneself, and antenatal 3d limit is raised, and just before giving birth feeds 2 times the day before yesterday, suckling about 6d free choice feeding.Medicine adding to mix homogeneously or drinking-water respectively in mixed feed shown in table 3 use, special messenger raise, environmental condition is basically identical, and timing is quantity-unlimiting feeds (do not waste with feedstuff and be as the criterion), freely drinks water.Routinely program carry out feeding, the cleaning of keep healthy immunity and environment.
Table 3 medicine addition and adding method
2.3 testing indexs
Environmental index: measure pig house temperature every day three times, early 8:00, noon 2:00, evening 6:00.2:00 reads psychrometer at noon, records humidity.Physical signs: at the body temperature of 2:00 mensuration sow at noon every day, mensuration Respiration Rate.Calculate sow daily ingestion amount, piglet daily gain, mortality rate etc..
2.4 data statistics processings
Test data Excel2003 arranges, and adopts the one factor analysis of variance program ANOVA of SPSS13.0 to carry out variance analysis, and result represents with average ± standard deviation.
3, result of the test
3.1 experimental period pig house gas epidemic disaster and physical signs
During whole test, pig house mean temperature reaches 32.08 DEG C, and average every daily maximum temperature reaches 36.54 DEG C, and relative humidity is normal is 56.1%, but have about more than half natural law humidity higher than 70%, it was shown that during test, brood sow has been in Heat-temperature stress state.During test every day sow Mean rectal temperature with respiratory frequency in Table 4.
Sow Mean rectal temperature and respiratory frequency every day during table 4 test
From table 4, sow body temperature is created certain impact by heat stress, reach the body temperature upper limit；The rectal temperature of medication A, C group pig body slightly decreases (p>0.05), the rectal temperature of all the other medication group pig bodies is all remarkably decreased (p<0.05), D group is substantially less than matched group and ABC group (p<0.05), showing can alleviate after using Chinese medicine the trend of body temperature rising, D group effect is particularly evident.Respiratory frequency then substantially declines (p < 0.05) than matched group, and after each medication group all has substantially reduction (p < 0.05) to show medication compared with matched group, sow Respiration Rate reduces, and hot panting is slowed down to some extent.Wherein, compare other medication group, the rectal temperature of medication D group pig body and respiratory frequency and decline the most notable.
The impact on sow feed intake of 3.2 Chinese medicine compositions
Sow feed intake is affected result in Table 5 by Chinese medicine composition.
The impact on sow feed intake of table 5 Chinese medicine composition
Note: different letter person represents significant difference (p < 0.05, Means ± SD) with column data mark.
From table 5, suckling 6d sow daily ingestion amount of giving a birth: D, E group is significantly higher than matched group (p < 0.05), and D group is significantly higher than B, C group (p < 0.05)；Suckling 7～14d sow daily ingestion amount: each test group is all significantly increased (p < 0.05) compared with matched group, and D, E group is significantly higher than A group；Suckling 14～21d sow daily ingestion amount: suckling 14～21d sow daily ingestion amount: each test group is all significantly increased (p < 0.05) compared with matched group, D group is apparently higher than A, C group (p < 0.05)；Age of sucking average sow daily ingestion amount: each test group is all significantly increased (p<0.05) compared with matched group, and D group is significantly higher than A, B, C group (p<0.05), without significant difference (p>0.05) between all the other each group.
The impact on weight gain of piglets of 3.3 Chinese medicine compositions
Weight gain of piglets is affected result in Table 6 by Chinese medicine composition.
Weight gain of piglets is affected result by table 6 Chinese medicine composition
Note: different letter person represents significant difference (p < 0.05, Means ± SD) with column data mark.
From table 6, matched group newborn piglet mortality rate is higher, reaches 5.36%；Mortality rate respectively 1.82%, 0,1.79%, 0,1.69% is respectively organized in medication.The original body mass of each group newborn piglet is without significant difference (p > 0.05)；During off-test, each group body weight is significantly higher than matched group (p < 0.05), and C group is significantly higher than A, C, E group (p < 0.05)；The 21d piglet average daily gain of each group is significantly higher than matched group (p < 0.05), and the average daily gain of D group to be significantly higher than A, C, E group (p < 0.05).
Claims (26)
1. the Chinese medicine composition preventing and treating heat stress of the animals, it is characterised in that be made up of the crude drug of following weight portion: Herba Hedyotidis Diffusae 60-100 part, Herba Moslae 30-60 part, Borneolum Syntheticum 4-6 part, Pericarpium Citri Reticulatae 25-50 part, Fructus Crataegi 40-80 part and Radix Glycyrrhizae 30-60 part.
2. Chinese medicine composition described in claim 1, it is characterised in that the weight portion of each crude drug is: Herba Hedyotidis Diffusae 67 parts, Herba Moslae 35 parts, Borneolum Syntheticum 4.2 parts, Pericarpium Citri Reticulatae 35 parts, Fructus Crataegi 45 parts, 35 parts of Radix Glycyrrhizae.
3. the Chinese medicine composition preventing and treating heat stress of the animals, it is characterized in that, be made up of the crude drug of following weight portion: Herba Hedyotidis Diffusae 60-100 part, Herba Moslae 30-60 part, Borneolum Syntheticum 4-6 part, Pericarpium Citri Reticulatae 25-50 part, Fructus Crataegi 40-80 part, Radix Glycyrrhizae 30-60 part, Radix Ophiopogonis 25-40 part and Herba Menthae 30-45 part.
4. Chinese medicine composition described in claim 3, it is characterised in that the weight portion of each crude drug is: Herba Hedyotidis Diffusae 67 parts, Herba Moslae 35 parts, Borneolum Syntheticum 4.2 parts, Pericarpium Citri Reticulatae 35 parts, Fructus Crataegi 45 parts, 35 parts of Radix Glycyrrhizae, Radix Ophiopogonis 30 parts, Herba Menthae 35 parts.
5. according to claim 1, Chinese medicine composition described in 2,3 or 4, it is characterised in that: described Chinese medicine composition is soluble powder.
6. the method preparing Chinese medicine composition described in claim 1 or 2, comprises the following steps:
(1) Herba Moslae and Pericarpium Citri Reticulatae pulverizing steam distillation being extracted volatile oil, volatile oil is standby；Extracting solution filters, and filtrate and medicinal residues are standby respectively；
(2) extract the medicinal residues after volatile oil and together carry out water with step (1) and carry after Herba Hedyotidis Diffusae, Fructus Crataegi and Radix Glycyrrhizae being pulverized, Aqueous extracts filters, filtrate reduced in volume is to extractum, add ethanol, stand, filter, filtrate reduced in volume, reclaim ethanol, obtain water extracting liquid, standby；
(3) in the volatile oil extracted, emulsifying agent is added, antitackiness agent, filler, stirring, high pressure homogenize, dry, obtain volatile oil soluble powder；
(4) adding filler and antitackiness agent in step (2) water extracting liquid, the dry water that obtains promotes extract powder；
(5) volatile oil soluble powder, water are promoted extract powder, finely divided ice and soluble carrier mix homogeneously, to obtain final product.
7. the method preparing Chinese medicine composition described in claim 3 or 4, comprises the following steps:
(1) Herba Moslae, Herba Menthae and Pericarpium Citri Reticulatae pulverizing steam distillation being extracted volatile oil, volatile oil is standby；Extracting solution filters, and filtrate and medicinal residues are standby respectively；
(2) extract the medicinal residues after volatile oil and together carry out water with step (1) and carry after Herba Hedyotidis Diffusae, Radix Ophiopogonis, Fructus Crataegi and Radix Glycyrrhizae being pulverized, Aqueous extracts filters, filtrate reduced in volume is to extractum, add ethanol, stand, filter, filtrate reduced in volume, reclaim ethanol, obtain water extracting liquid, standby；
(3) in the volatile oil extracted, add emulsifying agent, antitackiness agent and filler, stirring, high pressure homogenize, dry and obtain volatile oil soluble powder；
(4) adding filler and antitackiness agent in step (2) water extracting liquid, the dry water that obtains promotes extract powder；
(5) volatile oil soluble powder, water are promoted extract powder, finely divided ice and soluble carrier mix homogeneously, to obtain final product.
8. in accordance with the method for claim 6, it is characterised in that: step extracts volatile oil with steam distillation in (1)；In medical material, wherein add 10 times of quality water soakings after 1～2 hour, distillation extraction 3-8 hour；In step (2), filtrate reduced in volume to relative density is the extractum of 1.05-1.35, adds ethanol and makes alcohol content reach 60%.
9. in accordance with the method for claim 8, it is characterised in that: in step (1), in medical material, add 10 times of quality water soakings after 2 hours, distillation extraction 6 hours.
10. in accordance with the method for claim 7, it is characterised in that: step extracts volatile oil with steam distillation in (1)；In medical material, wherein add 10 times of quality water soakings after 1～2 hour, distillation extraction 3-8 hour；In step (2), filtrate reduced in volume to relative density is the extractum of 1.05-1.35, adds ethanol and makes alcohol content reach 60%.
11. in accordance with the method for claim 10, it is characterised in that: in step (1), in medical material, add 10 times of quality water soakings after 2 hours, distillation extraction 6 hours.
12. in accordance with the method for claim 6, it is characterised in that: step (3) adds the emulsifying agent accounting for volatile oil gross mass 10-25% in the volatile oil that step (1) is extracted.
13. in accordance with the method for claim 12, it is characterised in that: step (3) adds the emulsifying agent accounting for volatile oil gross mass 20% in the volatile oil that step (1) is extracted.
14. in accordance with the method for claim 7, it is characterised in that: step (3) adds the emulsifying agent accounting for volatile oil gross mass 10-25% in the volatile oil that step (1) is extracted.
15. in accordance with the method for claim 14, it is characterised in that: step (3) adds the emulsifying agent accounting for volatile oil gross mass 20% in the volatile oil that step (1) is extracted.
16. in accordance with the method for claim 6, it is characterised in that: the emulsifying agent described in step (3) is made up of Tween-80 and Span-80；Counting in mass ratio, the ratio of Tween-80 and Span-80 is 1-6:1.
17. in accordance with the method for claim 16, it is characterised in that: described emulsifying agent, to count in mass ratio, the ratio of Tween-80 and Span-80 is 1-4:1.
18. in accordance with the method for claim 17, it is characterised in that: described emulsifying agent, to count in mass ratio, the ratio of Tween-80 and Span-80 is 2:1.
19. in accordance with the method for claim 7, it is characterised in that: the emulsifying agent described in step (3) is made up of Tween-80 and Span-80；Counting in mass ratio, the ratio of Tween-80 and Span-80 is 1-6:1.
20. in accordance with the method for claim 19, it is characterised in that: described emulsifying agent, to count in mass ratio, the ratio of Tween-80 and Span-80 is 1-4:1.
21. in accordance with the method for claim 20, it is characterised in that: described emulsifying agent, to count in mass ratio, the ratio of Tween-80 and Span-80 is 2:1.
22. in accordance with the method for claim 6, it is characterized in that: step (3) adds the antitackiness agent accounting for volatile oil gross mass 0.5-1.5% in the volatile oil extracted, add amount of solid and account for the filler of volatile oil gross mass 75-250%, stirring, high pressure homogenize, spray drying, obtains volatile oil soluble powder；
Adding in water extracting liquid in step (4) and account for water extracting liquid gross mass 10-30% filler and account for the antitackiness agent of water extracting liquid gross mass 0.2-1%, spray drying obtains water and promotes extract powder；
Described antitackiness agent is magnesium stearate or Pulvis Talci；Described antitackiness agent include but not limited in Pulvis Talci, micropowder silica gel, magnesium stearate or Magnesiumaluminumsilicate any one or multiple；
Described filler includes but not limited to one or more mixing in soluble starch, maltodextrin, lactose, mannitol, glucose, sorbitol or sucrose；
Described soluble carrier includes but not limited to one or more mixing in soluble starch, maltodextrin, lactose, mannitol, glucose, sorbitol or sucrose；
Described filler includes but not limited to one or more in arabic gum, corn syrup, maltodextrin or chitosan.
23. in accordance with the method for claim 7, it is characterized in that: step (3) adds the antitackiness agent accounting for volatile oil gross mass 0.5-1.5% in the volatile oil extracted, add amount of solid and account for the filler of volatile oil gross mass 75-250%, stirring, high pressure homogenize, spray drying, obtains volatile oil soluble powder；
Adding in water extracting liquid in step (4) and account for water extracting liquid gross mass 10-30% filler and account for the antitackiness agent of water extracting liquid gross mass 0.2-1%, spray drying obtains water and promotes extract powder；
Described antitackiness agent is magnesium stearate or Pulvis Talci；Described antitackiness agent include but not limited in Pulvis Talci, micropowder silica gel, magnesium stearate or Magnesiumaluminumsilicate any one or multiple；
Described filler includes but not limited to one or more mixing in soluble starch, maltodextrin, lactose, mannitol, glucose, sorbitol or sucrose；
Described soluble carrier includes but not limited to one or more mixing in soluble starch, maltodextrin, lactose, mannitol, glucose, sorbitol or sucrose；
Described filler includes but not limited to one or more in arabic gum, corn syrup, maltodextrin or chitosan.
24. claim 1, the purposes in the medicine of preparation prevention or treatment heat stress of the animals of the Chinese medicine composition described in 2,3 or 4.
25. the purposes described in claim 24, it is characterised in that: described animal is domestic animal.
26. the purposes described in claim 25, it is characterised in that: described domestic animal is pig.
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| CN105560187A (en) * | 2014-11-11 | 2016-05-11 | 天津嘉创生物科技有限公司 | Preparation method of amoxicillin soluble powder |
| CN104758556B (en) * | 2014-12-10 | 2018-09-04 | 北京康华远景科技股份有限公司 | A kind of compound preparation and preparation method thereof of anti-Heat stress |
| CN105166418A (en) * | 2015-10-13 | 2015-12-23 | 临沂大学 | Anti-heat stress feed additives for rabbits and preparation method thereof |
| CN106668597A (en) * | 2016-12-27 | 2017-05-17 | 广州华农大实验兽药有限公司 | Anti-stress compound traditional Chinese veterinary medicine oral solution for livestock and poultry and preparation method and application thereof |
| CN113521180A (en) * | 2021-09-06 | 2021-10-22 | 武汉华扬动物药业有限责任公司 | Anti-heat stress traditional Chinese medicine oral liquid for pigs and preparation method thereof |
| CN114982875A (en) * | 2021-10-22 | 2022-09-02 | 东北农业大学 | Traditional Chinese medicine feed additive for relieving heat stress of laying hens and preparation method |
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| CN101979048A (en) * | 2010-10-25 | 2011-02-23 | 湖南敬和堂制药有限公司 | Medicinal composition of lonicera confusa and preparation method thereof |
| CN102885964A (en) * | 2011-07-20 | 2013-01-23 | 天津瑞贝特科技发展有限公司 | Traditional Chinese medicine composition with effect on clearing summer-heat and preparation method thereof |
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