CN104758556B - A kind of compound preparation and preparation method thereof of anti-Heat stress - Google Patents
A kind of compound preparation and preparation method thereof of anti-Heat stress Download PDFInfo
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Abstract
The present invention relates to a kind of compound preparations and preparation method thereof of anti-Heat stress, belong to additive agent for feeding field.The present invention is made of raw materials such as rhizoma anemarrhenae, folium cortex eucommiae, hawthorn, dried orange peel, centella, the vine of multiflower knotweed, Radix Glycyrrhizae, vitamins, and Chinese herb crushes addition ethyl alcohol respectively or water extraction is detached, concentrated, drying is prepared.Inventive formulation is reasonable, and the extracting method of modernization is utilized in Chinese medicine extraction process, makes effectively becoming in raw material that can extract to the greatest extent.The present invention has the effect of clearing heat and detoxicating, antibacterial anti-inflammatory help, calmness, tranquilizing the mind, anti-oxidant, resisting stress.
Description
Technical field
A kind of compound preparation and preparation method thereof of anti-Heat stress, belongs to additive agent for feeding field.
Background technology
Livestock and poultry is a kind of homeothermal animal, in certain comfort zone temperature range, can pass through the new old generation of itself
It thanks to generate thermal energy and stablize body temperature, and heat can be discharged in the state that ambient temperature increases to carry out body heat regulation,
If but environment temperature is more than the upper limit value of this comfort zone, that is, heat stress occurs.
Heat stress directly influences animal to be to reduce feed intake and nutritive digestibility and utilization rate, causes to grow
Performance declines;It indirectly influences then to be to inhibit immune response, increases the death rate, a series of economic losses thus brought.
But at present about improving the method for heat stress only simple addition vitamin, but effect be not it is highly desirable,
The present invention is mixed using Chinese medicine with vitamin, and the effect that vitamin is used alone is substantially increased.
Invention content
It is an object of the invention in view of the above-mentioned problems, provide it is a kind of can be in the anti-Heat stress of summer high temperature season
Compound preparation.
The technical scheme is that:A kind of compound preparation of anti-Heat stress, it is the original matched by following weight
Material is made:10-20 parts of rhizoma anemarrhenaes, 20-40 parts of folium cortex eucommiaes, 10-30 portions of hawthorn, 10-20 parts of dried orange peels, 10-20 parts of centellas, 10-30
Part vine of multiflower knotweed, 10-20 portions of Radix Glycyrrhizaes and a certain amount of vitamin composition.
A kind of compound preparation of anti-Heat stress, more preferably formula are:
12-18 parts of rhizoma anemarrhenaes, 24-36 parts of folium cortex eucommiaes, 12-28 portions of hawthorn, 12-18 parts of dried orange peels, 12-18 parts of centellas, 18-28
Part vine of multiflower knotweed, 12-18 portions of Radix Glycyrrhizaes and a certain amount of vitamin composition.
The highly preferred pharmaceutical formulation of the present invention is:
15 parts of rhizoma anemarrhenaes, 20 parts of folium cortex eucommiaes, 15 portions of hawthorn, 15 parts of dried orange peels, 15 parts of centellas, 20 parts of vines of multiflower knotweed, 15 portions of Radix Glycyrrhizaes
It is formed with a certain amount of vitamin.
A kind of preparation method of the compound preparation of anti-Heat stress provided by the present invention, includes the following steps:
Rhizoma anemarrhenae, folium cortex eucommiae, hawthorn, dried orange peel, centella, the vine of multiflower knotweed, Radix Glycyrrhizae are distinguished selected impurity elimination by a, are weighed, are cleaned, dividing
It does not crush, coarse powder is made;
Rhizoma anemarrhenae coarse powder is that 60%-80% ethanol waters extract 1-3 times, each 0.3-1h with volume ratio by b, each feed liquid
Than 1:8-1:10, merging filtrate, recycling ethyl alcohol obtains extract to the greatest extent;
Cortex Eucommiae leaf coarse powder is that 50%-70% ethanol waters extract 2-4 times, each 2-3h with volume ratio by c, each feed liquid
Than being 1:10-1:12, it filters, merging filtrate, filtrate recycling ethanol obtains extract to the greatest extent;
Haw pulp coarse powder and centella coarse powder are 70%-90% ethanol waters extraction 2-4 times with volume ratio by d, often
Secondary 1-3h, each solid-liquid ratio are 1:40-1:60, it filters, merging filtrate, filtrate recycling ethanol obtains extract to the greatest extent;
Dried orange peel coarse powder is that 60%-80% ethanol waters extract 1-3 times, each 2-4h with volume ratio by e, each solid-liquid ratio
It is 1:9-1:11, it filters, merging filtrate, filtrate recycling ethanol obtains extract to the greatest extent;
Vine of multiflower knotweed coarse powder is 60%-80% ethanol waters extraction 2-4 times, each 0.5-1.5h with volume ratio by f, every time
Solid-liquid ratio is 1:10-1:20, it filters, merging filtrate, filtrate recycling ethanol obtains extract to the greatest extent;
Reflux 1-3 times, each 2-3h is extracted with water in Radix Glycyrrhizae coarse powder by g, and each solid-liquid ratio is 1:10-1:12, it filters, merges
Extracting solution isolates and purifies precipitation, centrifuges, obtains extract;
The extract that h prepares b, c, d, e, f, g step process is added auxiliary material appropriate, is made according to a certain amount of proportioning
Pulvis, granule, oral solution.
Prescription foundation of the present invention
Theory of traditional Chinese medical science thinks that high temperature, which causes a disease, generates heat symptom-complex, i.e. heat stress syndrome.With Chinese and western medical science theory to heat stress
Syndrome analysis, it is believed that heat stress intensity constantly enhances, then digestion can be caused to die of exhaustion and died.Therefore it is answered from its control principle
" treating heat with cold drug ", " treatment of deficiency-syndrome by reinforcement ".This prescription has clearing heat and detoxicating, antibacterial anti-inflammatory help and tranquilizing and allaying excitement and other effects, and alleviation is answered
Swash reaction, adjust human body immune function, improves production performance.Rhizoma anemarrhenae clearing heat-fire, nourishing Yin and moistening dryness, clearing away the stomach-heat, nourishing stomach-YIN.Cortex Eucommiae
Leaf tonifying liver kidney yin, strengthening the bones and muscles, vine of multiflower knotweed nourishing blood and tranquilization.Papillary is used for the deficiency of liver-yin and kidney-yin, weakness of the spleen and the stomach, lassitude hypodynamia, palpitaition
Shortness of breath.Hawthorn digesting de-swelling, promoting the circulation of qiization are become silted up, and are used for fullness and oppression of chest and abdomen, do not feel like eating, dried orange peel qi-regulating, in tune, eliminating dampness, and stagnation resolvation, equally
Fullness and oppression of chest and abdomen, do not feel like eating can be treated, Papillary improves feed intake.Centella clearing heat and promoting diuresis, removing toxicity for detumescence.For damp and hot
Jaundice, heatstroke diarrhea, urolithiasis blood strangury, carbuncle sore tumefacting virus.Radix Glycyrrhizae enriching spleen-QI, nourishing heart, clearing heat and detoxicating, licoflavone have anti-inflammatory, solution
Convulsion, antiacid effect.Vitamin has anti-oxidation function, can effectively prevent heat stress.
The present invention is on the basis of a large amount of practical applications, according to traditional traditional Chinese medicine principles of formulating prescriptions, in conjunction with present prevalence
Disease learns principle, and the prescription of the animal resisting stress of screening has fine curative effect.
Specific implementation mode
Embodiment 1
10 parts of rhizoma anemarrhenaes, 20 parts of folium cortex eucommiaes, 10 portions of hawthorn, 10 parts of dried orange peels, 10 parts of centellas, 10 parts of vines of multiflower knotweed, 10 portions of Radix Glycyrrhizaes
It is formed with a certain amount of vitamin.
The preparation method of the compound preparation of anti-Heat stress provided by the present invention, includes the following steps:
Rhizoma anemarrhenae, folium cortex eucommiae, hawthorn, dried orange peel, centella, the vine of multiflower knotweed, Radix Glycyrrhizae are distinguished selected impurity elimination by a, are weighed, are cleaned, dividing
It does not crush, coarse powder is made;
B extracts 60% (v/v) ethanol water of rhizoma anemarrhenae coarse powder 1 time, each 0.3h, each solid-liquid ratio 1:8.Merge filter
Liquid, recycling ethyl alcohol obtain extract to the greatest extent;
C extracts 50% (v/v) ethanol water of Cortex Eucommiae leaf coarse powder 2 times, each 2h, and each solid-liquid ratio is 1:10.It crosses
Filter, merging filtrate, filtrate recycling ethanol obtain extract to the greatest extent;
D extracts haw pulp coarse powder and 70% (v/v) ethanol water of centella coarse powder 2 times, each 1h, per defective material
Liquor ratio is 1:40.Filtering, merging filtrate, filtrate recycling ethanol obtain extract to the greatest extent;
E extracts 60% (v/v) ethanol water of dried orange peel coarse powder 1 time, each 2h, and each solid-liquid ratio is 1:9.Filtering,
Merging filtrate, filtrate recycling ethanol obtain extract to the greatest extent;
F extracts 60% (v/v) ethanol water of vine of multiflower knotweed coarse powder 2 times, each 0.5h, and each solid-liquid ratio is 1:10.
Filtering, merging filtrate, filtrate recycling ethanol obtain extract to the greatest extent;
The water that g measures Radix Glycyrrhizae coarse powder with 10 times extracts reflux 1h, extracts 2h every time, and filtering merges extracting solution, isolates and purifies
Precipitation centrifuges, and obtains extract;
Auxiliary material appropriate is added in the extract that h prepares b, c, d, e, f, g step process, and pulvis is made, granule, takes orally
Liquid.
Embodiment 2
15 parts of rhizoma anemarrhenaes, 30 parts of folium cortex eucommiaes, 20 portions of hawthorn, 15 parts of dried orange peels, 15 parts of centellas, 20 parts of vines of multiflower knotweed, 15 portions of Radix Glycyrrhizaes
It is formed with a certain amount of vitamin.
The preparation method of the compound preparation of anti-Heat stress provided by the present invention, includes the following steps:
Rhizoma anemarrhenae, folium cortex eucommiae, hawthorn, dried orange peel, centella, the vine of multiflower knotweed, Radix Glycyrrhizae are distinguished selected impurity elimination by a, are weighed, are cleaned, dividing
It does not crush, coarse powder is made;
B extracts 70% (v/v) ethanol water of rhizoma anemarrhenae coarse powder 2 times, each 0.5h, each solid-liquid ratio 1:9, merge filter
Liquid, recycling ethyl alcohol obtain extract to the greatest extent;
C extracts 60% (v/v) ethanol water of Cortex Eucommiae leaf coarse powder 3 times, each 2.5h, and each solid-liquid ratio is 1:11,
Filtering, merging filtrate, filtrate recycling ethanol obtain extract to the greatest extent;
D extracts haw pulp coarse powder and 80% (v/v) ethanol water of centella coarse powder 3 times, each 2h, per defective material
Liquor ratio is 1:50, it filters, merging filtrate, filtrate recycling ethanol obtains extract to the greatest extent;
E extracts 70% (v/v) ethanol water of dried orange peel coarse powder 2 times, each 3h, and each solid-liquid ratio is 1:10, filtering,
Merging filtrate, filtrate recycling ethanol obtain extract to the greatest extent;
F extracts 70% (v/v) ethanol water of vine of multiflower knotweed coarse powder 3 times, each 1h, and each solid-liquid ratio is 1:15, mistake
Filter, merging filtrate, filtrate recycling ethanol obtain extract to the greatest extent;
The water that g measures Radix Glycyrrhizae coarse powder with 11 times extracts reflux 2h, extracts 2.5h every time, and filtering merges extracting solution, and separation is pure
Change precipitation, centrifuge, obtains extract;
Auxiliary material appropriate is added in the extract that h prepares b, c, d, e, f, g step process, and pulvis is made, granule, takes orally
Liquid.
Embodiment 3
20 parts of rhizoma anemarrhenaes, 40 parts of folium cortex eucommiaes, 30 portions of hawthorn, 20 parts of dried orange peels, 20 parts of centellas, 30 parts of vines of multiflower knotweed, 20 portions of Radix Glycyrrhizaes
It is formed with a certain amount of vitamin.
The preparation method of the compound preparation of anti-Heat stress provided by the present invention, includes the following steps:
Rhizoma anemarrhenae, folium cortex eucommiae, hawthorn, dried orange peel, centella, the vine of multiflower knotweed, Radix Glycyrrhizae are distinguished selected impurity elimination by a, are weighed, are cleaned, dividing
It does not crush, coarse powder is made;
B extracts 80% (v/v) ethanol water of rhizoma anemarrhenae coarse powder 3 times, each 1h, each solid-liquid ratio 1:10.Merge filter
Liquid, recycling ethyl alcohol obtain extract to the greatest extent;
C extracts 70% (v/v) ethanol water of Cortex Eucommiae leaf coarse powder 4 times, each 3h, and each solid-liquid ratio is 1:12.It crosses
Filter, merging filtrate, filtrate recycling ethanol to most extract;
D extracts haw pulp coarse powder and 90% (v/v) ethanol water of centella coarse powder 4 times, each 3h, per defective material
Liquor ratio is 1:60.Filtering, merging filtrate, filtrate recycling ethanol obtain extract to the greatest extent;
E extracts 80% (v/v) ethanol water of dried orange peel coarse powder 3 times, each 4h, and each solid-liquid ratio is 1:11.Filtering,
Merging filtrate, filtrate recycling ethanol obtain extract to the greatest extent;
F extracts 80% (v/v) ethanol water of vine of multiflower knotweed coarse powder 4 times, each 1.5h, and each solid-liquid ratio is 1:20.
Filtering, merging filtrate, filtrate recycling ethanol obtain extract to the greatest extent;
G flows back the 12 times of water the measured extractions of Radix Glycyrrhizae coarse powder 3 times, carries 3h every time.Filtering merges extracting solution, isolates and purifies
Precipitation centrifuges, and obtains extract.
Auxiliary material appropriate is added in the extract that h prepares b, c, d, e, f, g step process, and pulvis is made, granule, takes orally
Liquid.
Test example
Influence of 1 present invention to chicken heat shock
1.1 test method
The blue brown laying hen in 180 seas, stochastic averagina is taken to be divided into 6 groups, every group 30:Respectively high temperature group and room temperature group, high temperature
Group is divided into as the high temperature embodiment of the present invention 1,2,3 groups of oral solution, VC groups and high temperature control group, and experiment grouping being shown in Table 1;Room temperature
Group at 22 DEG C, under 26 DEG C of -30 DEG C of environment raise by high temperature group.The whole feeding of experiment 10 days.It is grouped situation and is shown in Table 1
The grouping and its disposition of 1 chicken of table
1.2 test result
(1) production performance is influenced
The embodiment of the present invention 1,2,3 groups compared with high temperature control group, laying rate has been respectively increased 18.23%, 18.78%,
18.46%.VC groups laying rate, egg size have increase trend compared with control group (not medication group), but difference is not notable.VC groups are being produced
There is certain anti-heat stress effect in terms of egg rate, egg size, but be not as with obvious effects as the present invention.The first shape of the embodiment of the present invention 1,2,3
Gland functional activity is all very high, especially the thyroid function activity highest of embodiment 2.During entire experiment, the present invention is implemented
2 groups of feedstuff-meat ratios of example are minimum, and no laying hen is dead, shows that the production performance of the group is best.
(2) to the influence of small intestinal villous height
Villi height (V), Crypt depth (C) and the height of naps/Crypt depth (V/C) of small intestine are to weigh small intestinal digestion
The important indicator of absorption function, V/C can then reflect that the functional status of small intestine, ratio rising show that mucous membrane improves, function enhancing, than
Value decline then shows that mucous membrane is impaired, function reduction.Small intestinal villous height, has reacted the ability of intestinal absorption digestion, and high temperature can damage
Hinder enteron aisle, inhibits the absorption of poultry nutrition substance.Compared with the control group, the embodiment of the present invention 1,2,3 groups and VC groups improve
The height of naps of small intestine, and 2 groups of the embodiment of the present invention is with obvious effects.
Influence of 2 present invention to pig heat stress
2.1 test method
2.1.1 the selection and processing of experimental animal
36 weight of selection castrate public affairs close to the consistent healthy two-way cross of (about 25kg or so), kind (long × big)
Pig, hereditary basis similar principle close by weight are randomly divided into six groups, and every group of 6 pigs mark ear tag.Experimental period is 15
It, high temperature group takes lasting day to become high temperature during experiment (close to natural megathermal climate).Daily temperature becomes at 30-38 DEG C
Change, relative humidity 50%, alternating temperature program is shown in Table 3, and thermophilic group cabin temperature is 25 DEG C, relative humidity 50%.It is grouped situation
It is shown in Table 2
The grouping and its disposition of 2 grower pigs of table
Table 3 continues the Circadian variation for day becoming high temperature
2.1.2 daily ration and feeding management are tested
The daily timing feeding wet-mixing material of experiment pig, the morning 8:30-9:30, afternoon 16:30-17:It is freely eaten in 30, remaining
Time free water.Other management conventionally carry out.
2.1.3 sampling and measurement
Performance test:
(1) average daily gain (ADG):It weighs in experiment the 0th day, the 15th day morning, calculates average daily gain.
Daily ingestion amount (ADFI):Appetite is counted day by day by head.
(2) feedstuff-meat ratio:Daily ingestion amount/daily gain
2.2 result
2.2.1 the whole story weight and production performance of pig
Influence of the 4 varying environment temperature of table to the production performance of pig
As a result known to:Compared with high temperature control group, the embodiment of the present invention 1,2,3 groups compared with high temperature control group, average day
Weightening, average daily gain increase, and feedstuff-meat ratio reduces.Show that the present invention can significantly reduce the heat stress of pig, improves pig
Production performance, the effect of especially 2 prescription compatibility of embodiment is best.
Claims (5)
1. a kind of compound preparation of anti-Heat stress, it is characterised in that be prepared from the following raw materials in parts by weight:10-20 parts are known
Mother, 20-40 parts of folium cortex eucommiaes, 10-30 portions of hawthorn, 10-20 parts of dried orange peels, 10-20 parts of centellas, the 10-30 parts of vines of multiflower knotweed, 10-20 parts
Radix Glycyrrhizae and a certain amount of vitamin.
2. a kind of compound preparation of anti-Heat stress as described in claim 1, it is characterised in that by following parts by weight
Raw material is made:12-18 parts of rhizoma anemarrhenaes, 24-36 parts of folium cortex eucommiaes, 12-28 portions of hawthorn, 12-18 parts of dried orange peels, 12-18 parts of centellas, 18-
28 parts of vines of multiflower knotweed, 12-18 portions of Radix Glycyrrhizaes and a certain amount of vitamin.
3. a kind of compound preparation of anti-Heat stress as described in claim 1, it is characterised in that by more preferably weight
The raw material of number is made:15 parts of rhizoma anemarrhenaes, 20 parts of folium cortex eucommiaes, 15 portions of hawthorn, 15 parts of dried orange peels, 15 parts of centellas, 20 parts of vines of multiflower knotweed, 15
Part Radix Glycyrrhizae and a certain amount of vitamin.
4. compound preparation according to claim 1, it is characterised in that selected vitamin be vitamin C, vitamin E,
One or more of vitamin B1, vitamin B2, vitamin B6 are composed.
5. a kind of compound preparation of anti-Heat stress described in claim 3, it is characterised in that be made by the following method:
Rhizoma anemarrhenae, folium cortex eucommiae, hawthorn, dried orange peel, centella, the vine of multiflower knotweed, Radix Glycyrrhizae are distinguished selected impurity elimination by a, are weighed, are cleaned, difference powder
It is broken, coarse powder is made;
Rhizoma anemarrhenae coarse powder is that 60%-80% ethanol waters extract 1-3 times, each 0.3-1h with volume ratio by b, each solid-liquid ratio 1:
8-1:10, merging filtrate, recycling ethyl alcohol obtains extract to the greatest extent;
Cortex Eucommiae leaf coarse powder is that 50%-70% ethanol waters extract 2-4 times, each 2-3h with volume ratio by c, and each solid-liquid ratio is
1:10-1:12, it filters, merging filtrate, filtrate recycling ethanol obtains extract to the greatest extent;
Haw pulp coarse powder and centella coarse powder are that 70%-90% ethanol waters extract 2-4 times with volume ratio by d, each 1-
3h, each solid-liquid ratio are 1:40-1:60, it filters, merging filtrate, filtrate recycling ethanol obtains extract to the greatest extent;
Dried orange peel coarse powder is that 60%-80% ethanol waters extract 1-3 times, each 2-4h with volume ratio by e, and each solid-liquid ratio is 1:
9-1:11, it filters, merging filtrate, filtrate recycling ethanol obtains extract to the greatest extent;
Vine of multiflower knotweed coarse powder is that 60%-80% ethanol waters extract 2-4 times, each 0.5-1.5h with volume ratio by f, each feed liquid
Than being 1:10-1:20, it filters, merging filtrate, filtrate recycling ethanol obtains extract to the greatest extent;
Reflux 1-3 times, each 2-3h is extracted with water in Radix Glycyrrhizae coarse powder by g, and each solid-liquid ratio is 1:10-1:12, filtering merges extraction
Liquid isolates and purifies precipitation, centrifuges, obtains extract;
The extract that h prepares b, c, d, e, f, g step process is added suitable vitamin and mixes, and auxiliary material appropriate is added, is made
Pulvis, granule, oral solution.
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CN105124162A (en) * | 2015-07-31 | 2015-12-09 | 郑州赛科药业科技有限公司 | Heat stress resistant compound preparation and preparation method thereof |
CN106309879A (en) * | 2016-10-20 | 2017-01-11 | 中悦民安(北京)科技发展有限公司 | Drug combination capable of resisting animal stress and preparation method thereof |
CN107373077A (en) * | 2017-08-24 | 2017-11-24 | 浙江群大饲料科技股份有限公司 | A kind of additive premix for preventing and treating broiler chicken heat stress and preparation method thereof |
CN111544559A (en) * | 2020-05-25 | 2020-08-18 | 河南省农业科学院畜牧兽医研究所 | Traditional Chinese medicine preparation for preventing and treating sheep transport stress syndrome and preparation method thereof |
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