CN104758556A - Compound preparation for resisting heat stress of animals and preparation method thereof - Google Patents
Compound preparation for resisting heat stress of animals and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a compound preparation for resisting heat stress of animals and a preparation method thereof, belonging to the field of feed additives. The compound preparation is composed of the following traditional Chinese medicinal raw materials: anemarrhena, eucommia leaf, hawthorn fruit, dried orange peel, gotu kola, vine of multiflower knotweed, licorice, vitamin and the like. The compound preparation is prepared by separately crushing the traditional Chinese medicinal raw materials, adding ethanol or water for extraction and successively carrying out separation, concentration and drying. The formula of the compound preparation is reasonable; modern extraction techniques are employed for extraction of the traditional Chinese medicinal raw materials, so effective components in the traditional Chinese medicinal raw materials can be extracted to a greatest extent; and the compound preparation the curative effects of clearing of heat and toxic materials, antibiosis, antiphlogosis, tonifying, calming, tranquillizing, antioxidation and anti-stress.
Description
Technical field
Compound preparation of a kind of anti-heat stress of the animals and preparation method thereof, belongs to additive agent for feeding field.
Background technology
Livestock and poultry is a kind of Homoiotherm, in certain comfort zone temperature range, can heat energy be produced by the metabolism of self and stablize body temperature, and discharge heat to carry out thermoregulation under the state that can raise in ambient temperature, if but ambient temperature exceedes the higher limit of this comfort zone, namely occurs heat stress.
Heat stress directly affects animal and is to reduce feed intake and nutritive digestibility and utilization rate, causes growth performance to decline; Indirectly affect, be to inhibit immunne response, increase mortality rate, a series of economic losses brought thus.
But just simply add vitamin about the method improving heat stress at present, but effect is not very desirable, the present invention adopts Chinese medicine to mix with vitamin, substantially increases the effect being used alone vitamin.
Summary of the invention
The object of the invention is to for the problems referred to above, provide a kind of can at the compound preparation of summer high temperature anti-heat stress of the animals in season.
Technical scheme of the present invention is: a kind of compound preparation of anti-heat stress of the animals, and it is made up of the raw material of following weight proportioning: 10-20 part Rhizoma Anemarrhenae, 20-40 part Folium Eucommiae, 10-30 part Fructus Crataegi, 10-20 part Pericarpium Citri Reticulatae, 10-20 part Herba Centellae, 10-30 part Caulis Polygoni Multiflori, 10-20 part Radix Glycyrrhizae and a certain amount of vitamin form.
A compound preparation for anti-heat stress of the animals, more preferred formula is:
12-18 part Rhizoma Anemarrhenae, 24-36 part Folium Eucommiae, 12-28 part Fructus Crataegi, 12-18 part Pericarpium Citri Reticulatae, 12-18 part Herba Centellae, 18-28 part Caulis Polygoni Multiflori, 12-18 part Radix Glycyrrhizae and a certain amount of vitamin composition.
The highly preferred pharmaceutical formulation of the present invention is:
15 parts of Rhizoma Anemarrhenaes, 20 parts of Folium Eucommiaes, 15 portions of Fructus Crataegis, 15 parts of Pericarpium Citri Reticulataes, 15 parts of Herba Centellaes, 20 parts of Caulis Polygoni Multiflori, 15 portions of Radix Glycyrrhizaes and a certain amount of vitamin compositions.
The preparation method of the compound preparation of a kind of anti-heat stress of the animals provided by the present invention, comprises the following steps:
A, by the Rhizoma Anemarrhenae, Folium Eucommiae, Fructus Crataegi, Pericarpium Citri Reticulatae, Herba Centellae, Caulis Polygoni Multiflori, Radix Glycyrrhizae selected roguing respectively, weighs, cleans, pulverizes respectively, makes coarse powder;
B is by Rhizoma Anemarrhenae coarse powder 60-80 (v/v) ethanol extraction 1-3 time, and 0.3-1h/ time, each solid-liquid ratio 8-10 doubly.Merging filtrate, reclaims ethanol to the greatest extent, obtains extract;
C is by Folium Eucommiae coarse powder 50-70 (v/v) ethanol extraction 2-4 time, and 2-3h/ time, each solid-liquid ratio is 10-12 times amount.Filter, merging filtrate, filtrate recycling ethanol is to most extract;
D is by haw pulp coarse powder and Herba Centellae coarse powder 70-90 (v/v) ethanol extraction 2-4h/ time, and 1-3h/ time, each solid-liquid ratio is 40-60 times amount.Filter, merging filtrate, filtrate recycling ethanol, to the greatest extent, obtains extract;
E by Pericarpium Citri Reticulatae coarse powder 60-80 (v/v) ethanol extraction 1-3 time, each 2-4h, each 9-11 times amount.Filter, merging filtrate, filtrate recycling ethanol, to the greatest extent, obtains extract;
F by Caulis Polygoni Multiflori coarse powder 60-80 (v/v) ethanol extraction 2-4 time, each 0.5-1.5h, each 10-20 times amount.Filter, merging filtrate, filtrate recycling ethanol, to the greatest extent, obtains extract;
G, by the water extraction backflow 1-3h of Radix Glycyrrhizae coarse powder by 10-12 times amount, extracts 2-3h/ time at every turn.Filter merge extractive liquid, separation and purification precipitation, centrifugalize, obtain extract;
The extract that b, c, d, e, f, g step process is prepared by h, according to a certain amount of proportioning, adds suitable adjuvant, makes powder, granule, oral liquid.
Prescription foundation of the present invention
Theory of Chinese medical science is thought, high temperature causes a disease and produces heat symptom-complex, i.e. heat stress syndrome.Use Chinese and western medical science theoretical to heat stress syndrome analysis, think that heat stress intensity constantly strengthens, then digestion can be caused to die of exhaustion and die.Therefore should " treating heat syndrome with cold-natured drugs ", " treatment of deficiency-syndrome by reinforcement " from its control principle.This prescription has the effects such as heat-clearing and toxic substances removing, anti-inflammation tonification and tranquillizing and allaying excitement, alleviates stress, regulates human body immune function, improves fertility performance.Rhizoma Anemarrhenae clearing away heat-fire, nourshing Yin and drynsessmoistening prescription, clearing away stomach-heat, nourishing stomach-YIN.Folium Eucommiae tonifying liver kidney yin, bone and muscle strengthening, Caulis Polygoni Multiflori nourishing blood to tranquillize the mind.Papillary is used for the hepatic and renal YIN deficiency, weakness of the spleen and stomach, fatigue and weakness, shortness of breath and palpitation.Hawthorn digesting de-swelling, circulation of qi promotingization are become silted up, and for distension and fullness of the chest and abdomen, anorexia, Pericarpium Citri Reticulatae is regulated the flow of vital energy, and in tune, dampness, blood stasis dispelling, can treat distension and fullness of the chest and abdomen, anorexia equally, and Papillary improves feed intake.Herba Centellae clearing away heat-damp and promoting diuresis, removing toxic substances and promoting subsidence of swelling.For jaundice due to damp-heat, heatstroke is suffered from diarrhoea, stranguria caused by urinary stone stranguria with blood, carbuncle sore tumefacting virus.Radix Glycyrrhizae enriching spleen-QI, nourish heart, heat-clearing and toxic substances removing, licoflavone have antiinflammatory, spasmolytic, antiacid effect.Vitamin has anti-oxidation function, effectively can prevent heat stress.
The present invention is on the basis of a large amount of practical application, and according to traditional Chinese medicine principles of formulating prescriptions, in conjunction with present epidemiology principle, the prescription of the animal anti-stress of screening, has good curative effect.
Detailed description of the invention
Embodiment 1
10 parts of Rhizoma Anemarrhenaes, 20 parts of Folium Eucommiaes, 10 portions of Fructus Crataegis, 10 parts of Pericarpium Citri Reticulataes, 10 parts of Herba Centellaes, 10 parts of Caulis Polygoni Multiflori, 10 portions of Radix Glycyrrhizaes and a certain amount of vitamin compositions.
The preparation method of the compound preparation of anti-heat stress of the animals provided by the present invention, comprises the following steps:
A, by the Rhizoma Anemarrhenae, Folium Eucommiae, Fructus Crataegi, Pericarpium Citri Reticulatae, Herba Centellae, Caulis Polygoni Multiflori, Radix Glycyrrhizae selected roguing respectively, weighs, cleans, pulverizes respectively, makes coarse powder;
B by Rhizoma Anemarrhenae coarse powder 60 (v/v) ethanol extraction 1 time, 0.3h/ time, each solid-liquid ratio 8 times.Merging filtrate, reclaims ethanol to the greatest extent, obtains extract;
C is by Folium Eucommiae coarse powder 50 (v/v) ethanol extraction 2 times, and 2h/ time, each solid-liquid ratio is 10 times amount.Filter, merging filtrate, filtrate recycling ethanol is to using up to obtain extract;
D is by haw pulp coarse powder and Herba Centellae coarse powder 70 (v/v) ethanol extraction 2h/ time, and 1h/ time, each solid-liquid ratio is 40 times amount.Filter, merging filtrate, filtrate recycling ethanol, to the greatest extent, obtains extract;
E by Pericarpium Citri Reticulatae coarse powder 60 (v/v) ethanol extraction 1 time, each 2h, each 9 times amount.Filter, merging filtrate, filtrate recycling ethanol, to the greatest extent, obtains extract;
F by Caulis Polygoni Multiflori coarse powder 60 (v/v) ethanol extraction 2 times, each 0.5h, each 10 times amount.Filter, merging filtrate, filtrate recycling ethanol, to the greatest extent, obtains extract;
G, by the water extraction backflow 1h of Radix Glycyrrhizae coarse powder by 10 times amount, extracts 2h/ time at every turn.Filter merge extractive liquid, separation and purification precipitation, centrifugalize, obtain extract;
The extract that b, c, d, e, f, g step process is prepared by h, adds suitable adjuvant, makes powder, granule, oral liquid.
Embodiment 2
15 parts of Rhizoma Anemarrhenaes, 30 parts of Folium Eucommiaes, 20 portions of Fructus Crataegis, 15 parts of Pericarpium Citri Reticulataes, 15 parts of Herba Centellaes, 20 parts of Caulis Polygoni Multiflori, 15 portions of Radix Glycyrrhizaes and a certain amount of vitamin compositions.
The preparation method of the compound preparation of anti-heat stress of the animals provided by the present invention, comprises the following steps:
A, by the Rhizoma Anemarrhenae, Folium Eucommiae, Fructus Crataegi, Pericarpium Citri Reticulatae, Herba Centellae, Caulis Polygoni Multiflori, Radix Glycyrrhizae selected roguing respectively, weighs, cleans, pulverizes respectively, makes coarse powder;
B by Rhizoma Anemarrhenae coarse powder 70 (v/v) ethanol extraction 2 times, 0.5h/ time, each solid-liquid ratio 9 times amount.Merging filtrate, reclaims ethanol to the greatest extent, obtains extract;
C is by Folium Eucommiae coarse powder 60 (v/v) ethanol extraction 3 times, and 2.5h/ time, each solid-liquid ratio is 11 times amount.Filter, merging filtrate, filtrate recycling ethanol is to most extract;
D is by haw pulp coarse powder and Herba Centellae coarse powder 80 (v/v) ethanol extraction 3h/ time, and 2h/ time, each solid-liquid ratio is 50 times amount.Filter, merging filtrate, filtrate recycling ethanol, to the greatest extent, obtains extract;
E by Pericarpium Citri Reticulatae coarse powder 70 (v/v) ethanol extraction 2 times, each 3h, each 10 times amount.Filter, merging filtrate, filtrate recycling ethanol, to the greatest extent, obtains extract;
F by Caulis Polygoni Multiflori coarse powder 70 (v/v) ethanol extraction 3 times, each 1h, each 15 times amount.Filter, merging filtrate, filtrate recycling ethanol, to the greatest extent, obtains extract;
G, by the water extraction backflow 2h of Radix Glycyrrhizae coarse powder by 11 times amount, extracts 2.5h/ time at every turn.Filter merge extractive liquid, separation and purification precipitation, centrifugalize, obtain extract;
The extract that b, c, d, e, f, g step process is prepared by h, adds suitable adjuvant, makes powder, granule, oral liquid.
Embodiment 3
20 parts of Rhizoma Anemarrhenaes, 40 parts of Folium Eucommiaes, 30 portions of Fructus Crataegis, 20 parts of Pericarpium Citri Reticulataes, 20 parts of Herba Centellaes, 30 parts of Caulis Polygoni Multiflori, 20 portions of Radix Glycyrrhizaes and a certain amount of vitamin compositions.
The preparation method of the compound preparation of anti-heat stress of the animals provided by the present invention, comprises the following steps:
A, by the Rhizoma Anemarrhenae, Folium Eucommiae, Fructus Crataegi, Pericarpium Citri Reticulatae, Herba Centellae, Caulis Polygoni Multiflori, Radix Glycyrrhizae selected roguing respectively, weighs, cleans, pulverizes respectively, makes coarse powder;
B by Rhizoma Anemarrhenae coarse powder 80 (v/v) ethanol extraction 3 times, 1h/ time, each solid-liquid ratio 10 times.Merging filtrate, reclaims ethanol to the greatest extent, obtains extract;
C is by Folium Eucommiae coarse powder 70 (v/v) ethanol extraction 4 times, and 3h/ time, each solid-liquid ratio is 12 times amount.Filter, merging filtrate, filtrate recycling ethanol is to most extract;
D is by haw pulp coarse powder and Herba Centellae coarse powder 90 (v/v) ethanol extraction 4h/ time, and 3h/ time, each solid-liquid ratio is 60 times amount.Filter, merging filtrate, filtrate recycling ethanol, to the greatest extent, obtains extract;
E by Pericarpium Citri Reticulatae coarse powder 80 (v/v) ethanol extraction 3 times, each 4h, each 11 times amount.Filter, merging filtrate, filtrate recycling ethanol, to the greatest extent, obtains extract;
F by Caulis Polygoni Multiflori coarse powder 80 (v/v) ethanol extraction 4 times, each 1.5h, each 20 times amount.Filter, merging filtrate, filtrate recycling ethanol, to the greatest extent, obtains extract;
G, by the water extraction backflow 3h of Radix Glycyrrhizae coarse powder by 12 times amount, carries 3h at every turn.Filter merge extractive liquid, separation and purification precipitation, centrifugalize, obtain extract.
The extract that b, c, d, e, f, g step process is prepared by h, adds suitable adjuvant, makes powder, granule, oral liquid.
Test example
1 the present invention is on the impact of chicken heat shock
1.1 test method
Get 180 blue brown laying hens in sea, stochastic averagina is divided into 6 groups, often organizes 30: be respectively high temperature group and room temperature group, high temperature group is divided into again the oral liquid of the high temperature embodiment of the present invention 1,2,3 groups, VC group and high temperature matched group, and test grouping is in table 1; Room temperature group is at 22 DEG C, and high temperature group is raised under 26 DEG C of-30 DEG C of environment.Test whole process is fed 10 days.Its grouping situation is in table 1
The grouping of table 1 chicken and disposal thereof
1.2 result of the test
(1) fertility performance is affected
The embodiment of the present invention 1,2,3 groups is compared with high temperature matched group, and laying rate improves 18.23%, 18.78%, 18.46% respectively.VC group laying rate, egg size have increase trend compared with matched group (not medication group), but difference is not remarkable.VC group has certain anti-heat stress effect in laying rate, egg size, but not as successful of the present invention.The thyroid function activity of the embodiment of the present invention 1,2,3 is all very high, and especially the thyroid function activity of embodiment 2 is the highest.In whole process of the test, the embodiment of the present invention 2 groups of feedstuff-meat ratios are minimum, dead without laying hen, show that the fertility performance of this group is best.
(2) on the impact of small intestinal villous height
The villi height (V) of small intestinal, Crypt depth (C) and height of naps/Crypt depth (V/C) are the important indicators weighing small intestinal digestive and absorptive functions, V/C then can reflect the functional status of small intestinal, ratio rises and shows that mucosa improves, function strengthens, ratio declines and then shows that mucosa is impaired, function reduction.Small intestinal villous height, reacted the ability of intestinal absorption digestion, high temperature can damage intestinal, suppresses the absorption of poultry nutrition material.Compared with matched group, the embodiment of the present invention 1,2,3 groups and VC group all improve the height of naps of small intestinal, and the embodiment of the present invention 2 groups of successfuls.
2 the present invention are on the impact of pig heat stress
2.1 test method
2.1.1 the selection of experimental animal and process
Select 36 body weight close to the galt of (about about 25kg), healthy two-way cross that kind is consistent (long × large), close by body weight, the principle that hereditary basis is similar, is divided into six groups at random, often organizes 6 pigs, labelling ear tag.Experimental period is 15 days, and duration of test high temperature group is taked to uprise temperature (megathermal climate of access expansion) lasting day.The temperature of every day is 30-38 DEG C of change, and relative humidity is 50%, and its alternating temperature program is in table 3, and thermophilic group cabin temperature is 25 DEG C, and relative humidity is 50%.Its grouping situation is in table 2
The grouping of table 2 grower pigs and disposal thereof
Table 3 continues the Circadian variation uprising temperature day
2.1.2 daily ration and feeding and management is tested
Experiment pig timing feeding every day wet-mixing material, the morning 8:30-9:30, free choice feeding in afternoon 16:30-17:30, all the other times freely drink water.Other management are conventionally carried out.
2.1.3 sampling and mensuration
Performance test:
(1) average daily gain (ADG): weigh test the 0th day, the 15th day morning, calculates average daily gain.
Daily ingestion amount (ADFI): add up appetite day by day by head.
(2) feedstuff-meat ratio: daily ingestion amount/daily gain
2.2 result
2.2.1 the body weight and fertility performance at the whole story of pig
Table 4 varying environment temperature is on the impact of the fertility performance of pig
Result is known: compared with high temperature matched group, and the embodiment of the present invention 1,2,3 groups is compared with high temperature matched group, and average daily gain, average daily ingestion amount all raise, and feedstuff-meat ratio reduces.Show, the present invention can reduce the heat stress of pig significantly, and improve the fertility performance of pig, especially the effect of embodiment 2 prescription compatibility is best.
Claims (5)
1. the compound preparation of an anti-heat stress of the animals, by the Rhizoma Anemarrhenae (Anemarrhena asphodeloides Bge.), Folium Eucommiae (Eucommiaulmoides Oliv.), Fructus Crataegi (Crataegus pinnatifida Bge.var.major N.E.Br., Crataegus pinnatifidaBge.), Pericarpium Citri Reticulatae (Citrus reticulataBlanco), Herba Centellae (Centellaasiatica (L.) Urb.), Caulis Polygoni Multiflori (Polygonummultiflorum Thunb.), Radix Glycyrrhizae (Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat, Glycyrrhizagla-bra L) composition, it is characterized in that being made up of the component of following parts by weight: 10-20 part Rhizoma Anemarrhenae, 20-40 part Folium Eucommiae, 10-30 part Fructus Crataegi, 10-20 part Pericarpium Citri Reticulatae, 10-20 part Herba Centellae, 10-30 part Caulis Polygoni Multiflori, 10-20 part Radix Glycyrrhizae and a certain amount of vitamin composition.
2., by the compound preparation of a kind of anti-heat stress of the animals according to claim 1, it is characterized in that being made up of the component of following parts by weight: 12-18 part Rhizoma Anemarrhenae, 24-36 part Folium Eucommiae, 12-28 part Fructus Crataegi, 12-18 part Pericarpium Citri Reticulatae, 12-18 part Herba Centellae, 18-28 part Caulis Polygoni Multiflori, 12-18 part Radix Glycyrrhizae and a certain amount of vitamin form.
3. by the compound preparation of a kind of anti-heat stress of the animals according to claim 1, the raw material of its more preferred parts by weight is made: 15 parts of Rhizoma Anemarrhenaes, 20 parts of Folium Eucommiaes, 15 portions of Fructus Crataegis, 15 parts of Pericarpium Citri Reticulataes, 15 parts of Herba Centellaes, 20 parts of Caulis Polygoni Multiflori, 15 portions of Radix Glycyrrhizaes and a certain amount of vitamin compositions.
4. the compound preparation according to claims 1, is characterized in that selected vitamin is that one or more combination in VC, VE, VB1, VB2, VB6 forms.
5. the compound preparation of a kind of anti-heat stress of the animals as claimed in claim 3, is characterized in that being obtained by following method:
A, by the Rhizoma Anemarrhenae, Folium Eucommiae, Fructus Crataegi, Pericarpium Citri Reticulatae, Herba Centellae, Caulis Polygoni Multiflori, Radix Glycyrrhizae selected roguing respectively, weighs, cleans, pulverizes respectively, makes coarse powder;
B by Rhizoma Anemarrhenae coarse powder 60-80 (v/v) ethanol extraction 1-3 time, 0.3-1h/ time, each solid-liquid ratio 8-10 times amount.Merging filtrate, reclaims ethanol to the greatest extent, obtains extract;
C is by Folium Eucommiae coarse powder 50-70 (v/v) ethanol extraction 2-4 time, and 2-3h/ time, each solid-liquid ratio is 10-12 times amount.Filter, merging filtrate, filtrate recycling ethanol is to most extract;
D is by haw pulp coarse powder and Herba Centellae coarse powder 70-90 (v/v) ethanol extraction 2-4h/ time, and 1-3h/ time, each solid-liquid ratio is 40-60 times amount.Filter, merging filtrate, filtrate recycling ethanol, to the greatest extent, obtains extract;
E by Pericarpium Citri Reticulatae coarse powder 60-80 (v/v) ethanol extraction 1-3 time, each 2-4h, each 9-11 times amount.Filter, merging filtrate, filtrate recycling ethanol, to the greatest extent, obtains extract;
F by Caulis Polygoni Multiflori coarse powder 60-80 (v/v) ethanol extraction 2-4 time, each 0.5-1.5h, each 10-20 times amount.Filter, merging filtrate, filtrate recycling ethanol, to the greatest extent, obtains extract;
G, by the water extraction backflow 1-3h of Radix Glycyrrhizae coarse powder by 10-12 times amount, extracts 2-3h/ time at every turn.Filter merge extractive liquid, separation and purification precipitation, centrifugalize, obtain extract;
Extract prepared by b, c, d, e, f, g step process is added appropriate vitamin mixing by h, adds suitable adjuvant, makes powder, granule, oral liquid.
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| CN105124162A (en) * | 2015-07-31 | 2015-12-09 | 郑州赛科药业科技有限公司 | Heat stress resistant compound preparation and preparation method thereof |
| CN106309879A (en) * | 2016-10-20 | 2017-01-11 | 中悦民安（北京）科技发展有限公司 | Drug combination capable of resisting animal stress and preparation method thereof |
| CN107373077A (en) * | 2017-08-24 | 2017-11-24 | 浙江群大饲料科技股份有限公司 | A kind of additive premix for preventing and treating broiler chicken heat stress and preparation method thereof |
| CN111544559A (en) * | 2020-05-25 | 2020-08-18 | 河南省农业科学院畜牧兽医研究所 | Traditional Chinese medicine preparation for preventing and treating sheep transport stress syndrome and preparation method thereof |
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| CN105124162A (en) * | 2015-07-31 | 2015-12-09 | 郑州赛科药业科技有限公司 | Heat stress resistant compound preparation and preparation method thereof |
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| CN107373077A (en) * | 2017-08-24 | 2017-11-24 | 浙江群大饲料科技股份有限公司 | A kind of additive premix for preventing and treating broiler chicken heat stress and preparation method thereof |
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| CN104758556B (en) | 2018-09-04 |
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