CN104138526B - A kind of root of three-nerved spicebush iron sheet ginseng granules agent - Google Patents
A kind of root of three-nerved spicebush iron sheet ginseng granules agent Download PDFInfo
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Abstract
The invention discloses a kind of root of three-nerved spicebush iron sheet ginseng granules agent, its prescription is composed of the following components:The mass parts of dendrobium candidum 10 40, the mass parts of the root of three-nerved spicebush 10 40, the mass parts of ginseng 10 40, in addition, the 1 10 mass parts Radixs Astragali, 1 10 mass parts Chinese yams, any one or a few in the 1 10 mass parts tubers of dwarf lilyturf can also be included, raw material is extracted, concentration, granule is made after granulation.The Chinese medicine and health food of the present invention is developed based on Traditional Chinese health Health preserving theory; scientific formulation is reasonable; preparation technology is simple; safety and stability is effective; there is assistant protection function with strengthen immunity and to chemical damage, it is adaptable to immunocompromised person and there is chemical damage danger person to take.
Description
Technical field
The present invention relates to traditional Chinese medicine health care product technical field, more particularly to one kind is in the multi-flavors such as the root of three-nerved spicebush, dendrobium candidum, ginseng
What medicinal material was constituted has strengthen immunity and has the Chinese herbal granules of assistant protection function to chemical damage.
Background technology
Hepatic injury is the common pathologic process of liver, thus a series of caused liver diseases such as liver fibrosis, hepatic sclerosis
And liver tumour etc. seriously endangers and arrives human health.Hepatic injury mainly includes chemical damage and immunological liver injury.Chemically
Hepatic injury, as the term suggests it is as the hepatic injury caused by chemically Hepatoxic substance.These chemical substances are included in alcohol, environment
Chemical toxicant and some drugses.They can cause cell membrane and enzyme to destroy, so as to cause hepatic dysfunction, cause liver
Apoptosis or necrosis, further cause the obstacle of whole liver function.With being showing improvement or progress day by day for society, living standards of the people are carried
Because bad eating habit (such as excessively taking in high-fat food and excessive drinking) is led in the high, change of dietary structure, crowd
Cause dyslipidemia person to increase year by year, cause liver to be damaged in various degree.Exploitation has and had to chemical damage as soon as possible
The health food of auxiliary protection function is necessary.
Found according to more than 50 national medical experts' researchs of the World Health Organization, the treatment best to various diseases or pre-
It is anti-.The inspection of current various hospitals can only be diagnosed to the disease produced, often again can be because finding once confirmation is ill
Feel simply helpless too late.Any high-tech is all difficult to make patient return to former state of falling ill.Therefore, it is preferable to doctor be not
Allow the doctor that patient falls ill.As long as any disease prevention and maintenance are first, the probability of morbidity will be reduced, it might even be possible to completion
Solve and avoid.It is the most important means of prevention disease to strengthen immunity of organisms.Traditional medicine emphasizes the effect of " healthy tendency " very much, recognizes
The growth and decline of good and evil struggle are both depended on for the generation of disease, development and conversion, so as to propose the base therapy of " strengthening vital QI to eliminate pathogenic factors "
Thinking, that is, emphasize, from entirety, to strengthen the immunity of body to resist with disease.Modern study and clinical practice are found, are permitted
It is many to play the role of to strengthen body's immunity with the help class natural plants strengthened the body resistance to consolidate the constitution, invigorating Qi and tonifying kidney is acted on.
The root of three-nerved spicebush is canella the root of three-nerved spicebush Lindera aggregata (Sims) Kosterm. root tuber.The root of three-nerved spicebush is pungent, property
Temperature.Return lung, spleen, kidney, bladder warp.The effect of with promoting qi circulation and relieving pain, warming kidney for dispelling cold, it is known that chemical composition is mainly volatilization in the root of three-nerved spicebush
Oil, morphinane alkaloid and furan sesquiterpene and its lactone three major types.The root of three-nerved spicebush has relatively broad pharmacological activity, including anti-
Bacterium, antivirus action;Protect liver shield stomach effect, suppresses digestive tract ulcer, and enhancing stomach motion rhythm and pace of moving things effect substantially Anti-ethanol can be lured
The cellular damage of hair, with cytoprotection.
Dendrobium candidum is orchid dendrobium candidum Dendrobium officinale Kimura et Migo drying
Stem, sweet, cold nature returns stomach, kidney channel.With reinforcing stomach reg fluid, the function of nourishing Yin and clearing heat.Modern medicine and pharmacology, which is studied, to be shown, iron sheet
The stem of noble dendrobium mainly contains polysaccharide, amino acid and trace element, the luxuriant and rich with fragrance chemical composition such as class and a small amount of alkaloid, be immunized with enhancing,
The pharmacological actions such as antifatigue, antitumor, hypoglycemic, protect liver shield stomach.
Ginseng is Araliaceae ginseng Panax ginseng C.A.Mey. drying root and rhizome, sweet, slight bitter, property
It is flat, returns spleen, lung, the heart channel of Hang-Shaoyin.With reinforcing vital energy, veins takes off admittedly reinforces the spleen to benefit the lung, and promotes the production of body fluid, the function of calming the nerves.Modern medicine and pharmacology is studied
Show, ginseng mainly contains a variety of ginsenosides, carbohydrate, volatile oil, amino acid, trace element and organic acid, multivitamin, courage
The composition such as alkali and enzyme.With antifatigue, strengthen immunity, antitumor, the hypoglycemic and anti-ageing effect of waiting for a long time.
The Radix Astragali is legume astragalus mongolicus Astragalus membranaceus (Fisch.) Bge var or film pod is yellow
The root of stilbene.Main product is in the Inner Mongol, Shanxi, the Heilungkiang of China and other places.The Radix Astragali is sweet, slightly warm in nature, Return liver, spleen, lung, kidney channel.Have
The effect of invigorating qi for consolidating superficies, arrest sweating admittedly take off, torr sore myogenic, inducing diuresis for removing edema.Modern study shows that the Radix Astragali has enhancing immunity of organism work(
Energy, liver protection, diuresis, anti-aging, resisting stress, decompression and wide antibacterial action.
Chinese yam is Dioscoreaceae plant Chinese yam Dioscorea opposite Thumb. dry rhizome, sweet, mild-natured, is returned
Spleen, lung, kidney channel.With tonifying spleen nourishing the stomach, engender liquid and benefic lung, the puckery smart function of kidney tonifying.Modern medicine and pharmacology research shows that Chinese yam contains many
Sugar, protein, dopamine, hydrochloric acid Chinese yam alkali, batatasins, vitamin, calcium, magnesium, copper and abundant iodine and other trace elements, with
And the chemical composition such as allantoin, saponin(e, choline.With hypoglycemic, strengthen immunity, anti-aging, reducing blood lipid, nourishing the stomach protect liver work(
Energy.
The tuber of dwarf lilyturf is liliaceous plant tuber of dwarf lilyturf Ophiopogon japonicus (L) dried root.Sweet, slight bitter is slightly cold.
The thoughts of returning home, lung, stomach.With nourishing Yin and promoting production of body fluid, the function of moistening lung clears away heart-fire.The tuber of dwarf lilyturf contains a variety of steroid saponins and flavone compound.
Modern pharmacological research shows that the tuber of dwarf lilyturf has the effects such as strengthen immunity, enhancing hypoxia-bearing capability, anti-arrhythmia.
The root of three-nerved spicebush, ginseng heat, dendrobium candidum are cold in nature, and three's compatibility is coordinated, reinforced vital energy as main ingredient, negative and positive, beneficial stomach shield
Liver.The complete square tonifying Qi effect after the Radix Astragali that adds more preferably, was adapted to deficiency of vital energy person and taken as ministerial drug the Radix Astragali, Chinese yam, the tuber of dwarf lilyturf;Add Chinese yam
Complete square tonifying spleen effect is notable afterwards, and suitable person with Spleen Deficiency is taken;Add complete square enriching yin effect after the tuber of dwarf lilyturf to strengthen, be adapted to deficiency of Yin person and take.
We both meet traditional Chinese medical theory according to the pharmacodynamic profile and the scope of application, reasonable combination of six kinds of Chinese medicine, have again modern pharmacology and
Clinical research foundation, does not have also, main cause is the inadequate system of formulating, simultaneously using them as the health food of combination at present
Also exist on preparation and production technology many difficult.
The content of the invention
In view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to a kind of root of three-nerved spicebush iron sheet ginseng granules agent is provided, the present invention
Root of three-nerved spicebush iron sheet ginseng granules can strengthen immunity, have assistant protection function to chemical damage.
The purpose of the present invention is achieved through the following technical solutions:A kind of root of three-nerved spicebush iron sheet ginseng granules agent, its prescription
It is composed of the following components:Dendrobium candidum 10-40 mass parts, root of three-nerved spicebush 10-40 mass parts, ginseng 10-40 mass parts.In addition, may be used also
With any one or a few including the 1-10 mass parts Radix Astragali, 1-10 mass parts Chinese yam, in the 1-10 mass parts tubers of dwarf lilyturf.
Animal Efficacy experiments are carried out with the root of three-nerved spicebush iron sheet ginseng granules agent of the present invention:
Strengthen immunity functional experiment sets basic, normal, high three dosage groups, be respectively equivalent to the actual intake of people 5,10,
30 times, mouse stomach, while setting blank control group (distilled water).The given the test agent of continuous 30 days orally administration various doses.It is real
Result is tested to show:Compared with control group, the enhancing of test sample group mice spleen lymphocytes proliferation ability, difference has conspicuousness, and (q is examined
Test, P < 0.05);Compared with control group, the rise of test sample group mouse spleen index, difference has conspicuousness, and (q is examined, P <
0.05);Compared with control group, the phagocytic index and phagocytic rate of test sample group Turnover of Mouse Peritoneal Macrophages phagocytosis chicken red blood cell are equal
Rise, difference has conspicuousness (q is examined, P < 0.05).Illustrate that the present invention has strengthen immunity function.
There is assistant protection function experiment to use carbon tetrachloride hepatic injury modelling to chemical damage, if basic, normal, high by three
Individual dosage group, be respectively equivalent to the actual intake of people 5,10,30 times, while setting blank control group (distilled water) and model comparison
Group.The given the test agent of continuous 30 days orally administrations mouse various dose.Test result indicates that:On the premise of model is set up, with
Model control group compares, and test sample group mouse AST, ALT are substantially reduced, and difference has conspicuousness (q is examined, P < 0.05);With
Model control group compares, and test sample group mouse liver cell degree of necrosis mitigates, and difference has conspicuousness, and (q is examined, P <
0.05)。
Toxicological safety experiment is carried out with the root of three-nerved spicebush iron sheet ginseng granules agent of the present invention:
Rat acute toxicity test is as follows:Using maximum tolerated dose method, by sample with 20.0g/kg body weight doses
10 each to male and female rat (180-220g) oral gavage contaminations.Weigh sample and be made into 0.5g/mL, it is secondary by 20mL/kg body weight
Gavage is given, and is spaced 4h.After contamination, general status, poisoning symptom and the death condition of rat are observed, two weeks time limits are observed.Examination
Test result:Sample is all higher than 20.0g/kg body weight to the maximum tolerated dose of the acute oral of male and female rat.
The experiment of chmice acute Oral toxicity is as follows:Using maximum tolerated dose method, by sample with 20.0g/kg body weight doses
10 each to male and female mouse (18-22g) oral gavage contaminations.Weigh sample and be made into 0.5g/mL, filled by 20mL/kg body weight is secondary
Stomach is given, and is spaced 4h.After contamination, general status, poisoning symptom and the death condition of mouse are observed, two weeks time limits are observed.Experiment
As a result:Sample is all higher than 20.0g/kg body weight to the maximum tolerated dose of the acute oral of male and female mouse.
Mouse marrow cell micro nuclear test is as follows:By sample respectively with tri- dosage of 2.5g/kg, 5.0g/kg, 10.0g/kg
Group, separately sets negative control (distilled water) and positive controls (endoxan 60mg/kg body weight), every group of 10 mouse (25-
30g, male and female half and half), give within 24 hours sample using interval, three dosage components also known as take sample to be made into 0.125,0.25,
0.50g/mL, by 20mL/kg body weight, respectively to each group animal gavage.Once a day, continuous 2 days.Last is put to death to 6h after sample
Animal, takes its bone marrow of sternum film-making, and methanol is fixed, Giemsa dyeing.Sample Bone Marrow cell micronucleus test result is feminine gender.
Sperm malformation test is as follows:If three dosage groups of sample 2.5g/kg, 5.0g/kg, 10.0g/kg body weight, separately set the moon
Property control (distilled water) and positive controls (mitomycin C 2.0mg/kg body weight), every group of 10 male mices (25-30g).Three
Individual dosage component also known as takes sample to be made into 0.125,0.25,0.50g/mL, by 20mL/kg body weight, respectively to each group animal gavage.
Once a day, continuous 5 days.The 35th day is given after sample in first, takes two side epididymis to shred, takes filtrate film-making.Microscopy is observed, per mouse meter
The lopsided number of 1000 sperms of number.Sample sperm malformation test result is negative.
Salmonella reversion test is as follows:From histidine auxotroph salmonella typhimurium TA97、TA98、TA100、TA102, adopt
Flat board incorporation methods are used, proof load is 40 μ g/ wares, 200 μ g/ wares, 1000 μ g/ wares, 5000 μ g/ wares.Directly count on culture medium
Returning for each bacterial strain becomes clump count.Detection is repeated once.Sample Salmonella reversion test result is feminine gender.
Feeding trial is as follows within 30 days:If basic, normal, high three dosage groups, are respectively equivalent to 25,50, the 100 of people's recommended amounts
Times, oral gavage gives rat, continuous 30 days.Blank control group separately is set, distilled water is given, free diet is continuous to feed 30 days.
Continuous Observation 30 days, records weekly body weight and food-intake, and calculates food utilization, and experiment end of term taking blood from jugular vein carries out blood
Learn and check, broken end takes blood to carry out blood bio-chemistry checking, internal organs gross examination of skeletal muscle is carried out to every rat, while taking liver,kidney,spleen, testis
(ovary) weighs and calculates dirty body ratio, and takes liver,kidney,spleen, stomach, intestines, testis (ovary) to carry out histopathologic examination.As a result:
Experimental animal growing state is good, hematological examination, biochemical analysis, main dirty body when histological indications and control group
Compare, equal no significant difference.
Above-mentioned acute toxicity test, micronucleus test, sperm malformation test, Salmonella reversion test, 30 days feeding trial results are shown
This product safety non-toxic.
Stability test is as follows:By three batches of samples by commercially available back through under 37~40 DEG C of temperature and 75% relative humidities
Place 3 months, the three batches of samples are carried out respectively 0 month, January, 2 months, March all events detections (including organoleptic indicator, Thick many candies,
It is total saposins, moisture, ash content, granularity, melting, lead, arsenic, mercury, BHC, DDT, net content minus deviation, total plate count, big
Intestinal flora, mould, saccharomycete, salmonella, Shigella, golden yellow Portugal coccus, hemolytic) etc., testing result is accorded with
Close the regulation of this product quality standard (company standard).
The beneficial effects of the invention are as follows:The present invention is aided with malt with dendrobium candidum, the root of three-nerved spicebush, ginseng, the Radix Astragali, Chinese yam, the tuber of dwarf lilyturf
The health food that people are willing to accept is made in dextrin, xylitol, and reasonable recipe both meets traditional Chinese medical theory, there is modern pharmacology again
And clinical research foundation;Root of three-nerved spicebush iron sheet ginseng granules agent prepared by the present invention has strengthen immunity, had to chemical damage
Assistant protection function, it is adaptable to immunocompromised person and there is chemical damage danger person to take, with well wide market
Prospect.
Embodiment
Root of three-nerved spicebush iron sheet ginseng granules agent of the present invention, its prescription is composed of the following components:Dendrobium candidum 10-40 mass parts, crow
Medicine 10-40 mass parts, ginseng 10-40 mass parts.In addition, it can include the 1-10 mass parts Radix Astragali, 1-10 mass parts Chinese yam, 1-
Any one or a few in the 10 mass parts tubers of dwarf lilyturf.
The root of three-nerved spicebush iron sheet ginseng granules agent is prepared by following technique:
(1) dendrobium candidum is extracted:Dendrobium candidum medicinal material is weighed by formula, in input multi-function extractor, adds 30 times of amounts
Water, is heated to boiling, is incubated 3 hours, filter pump goes out decoction;Second is carried out to extract, plus 30 times of amount water are extracted, boiling insulation 3
Hour, filter pump goes out decoction;Third time extraction is carried out, plus 30 times of amount water are extracted, boiling insulation 3 hours, filter pump goes out decoction, closes
And No. three extract solutions enter basin.
(2) other medicinal material extracts:The root of three-nerved spicebush, ginseng, the Radix Astragali, Chinese yam, the tuber of dwarf lilyturf are weighed by formula, put into multi-function extractor
In, 10 times of amount water are added, boiling is heated to, is incubated 2 hours, filter pump goes out decoction;Second is carried out to extract, plus 10 times of amount water extractions
Take, boiling insulation 2 hours, filter pump goes out decoction;Merge extract solution twice and enter basin.
(3) concentrate:Extract solution carries out being concentrated under reduced pressure into relative density 1.05 or so (60 DEG C of measurements).
(4) boiling granulating:Using maltodextrin and xylitol as auxiliary material, medicinal extract is sprayed into, boiling granulating obtains root of three-nerved spicebush iron sheet people
Ginseng grains.
(5) pack:Packed, labelled by defined specification.
The following detailed description of the specific embodiment of the present invention
Embodiment 1
Weigh in dendrobium candidum 3500g input multi-function extractors, add 30 times of amount water, be heated to boiling, insulation 3 is small
When, filter pump goes out decoction;Second is carried out to extract, plus 30 times of amount water are extracted, boiling insulation 3 hours, filter pump goes out decoction;Carry out
Third time is extracted, plus 30 times of amount water are extracted, boiling insulation 3 hours, and filter pump goes out decoction, merges No. three extract solutions and enters basin.Claim
Take root of three-nerved spicebush 3500g, ginseng 3000g to put into multi-function extractor, add 10 times of amount water, be heated to boiling, be incubated 2 hours, mistake
Filter pump goes out decoction;Second is carried out to extract, plus 10 times of amount water are extracted, boiling insulation 2 hours, filter pump goes out decoction;Merge twice
Extract solution enters basin.Extract solution carries out being concentrated under reduced pressure into relative density 1.05 or so (60 DEG C of measurements).With maltodextrin and xylose
Alcohol is auxiliary material, sprays into medicinal extract, and boiling granulating obtains root of three-nerved spicebush iron sheet ginseng granules.
Immunologic function result of the test:
Table 1:NK cytoactives, Splenic vein hemodynamics experimental result ()
Group (g/kg) | NK cytoactives (%) | Pouring turns OD differences |
Control group | 60.1±2.8 | 0.031±0.012 |
0.67 | 62.2±3.0 | 0.052±0.011* |
1.33 | 62.3±3.1 | 0.055±0.008* |
4.0 | 63.5±2.7 | 0.060±0.009* |
Note:* compared with control group, there is significant difference
From table 1, the NK cytoactives and control group phase of the mouse of each dosage group of root of three-nerved spicebush iron sheet ginseng granules of the present invention
Than difference that there are no significant;ConA induction mouse spleen lymphocyte transformation experiment in, the OD differences of each dosage group of sample with it is right
Compared according to group, there is significant difference (p<0.05).
Table 2:DTH, spleen index and thymus index experimental result ()
Group (g/kg) | Thymus index (%) | Spleen index (%) | Swelling (auris dextra-left ear) (mg) |
Control group | 0.22±0.03 | 0.42±0.07 | 9.0±1.1 |
0.67 | 0.29±0.03 | 0.43±0.05 | 10.3±1.0 |
1.33 | 0.28±0.04 | 0.51±0.05 | 10.8±2.3 |
4.0 | 0.30±0.02 | 0.66±0.06* | 10.2±2.0 |
Note:* compared with control group, there is significant difference
From table 2, the mouse spleen index of root of three-nerved spicebush iron sheet ginseng granules high dose group of the present invention has aobvious compared with control group
Write sex differernce (p<0.05).
The phagocytosis chicken red blood cell experiment of the macrophage of table 3
Group (g/kg) | Phagocytic index | Phagocytic rate (%) |
Control group | 3.45±0.68 | 20.25±3.90 |
0.67 | 4.01±0.32 | 23.00±3.51 |
1.33 | 4.71±0.89* | 31.02±3.37* |
4.0 | 5.02±0.60* | 33.57±3.28* |
Note:* compared with control group, there is significant difference;
From table 3, the middle and high dosage group phagocytic percentage of root of three-nerved spicebush iron sheet ginseng granules of the present invention and phagocytic index are with compareing
Group is compared, and has significant difference (p<0.05).
According to result judgement, this product has strengthen immunity function.
There is assistant protection function result of the test to chemical damage:
Influence of the table 4 to mice serum ALT, AST
Group (g/kg) | ALT(U/L) | AST(U/L) |
Blank control | 40±12 | 101±41 |
Model comparison | 4351±1212 | 6824±1560 |
0.67 | 4152±1524 | 5428±1695 |
1.33 | 2784±1387* | 4425±1527* |
4.0 | 2587±1392* | 4267±1499* |
Note:* compared with model control group, there is significant difference;
From table 4, compared with blank control group, model group mouse ALT, AST are significantly raised, compared with model group, in,
ALT, AST are substantially reduced in high dose group murine liver tissue, there is significant difference (p<0.05).
Influence of the table 5 to murine liver tissue Histopathologic changes
Note:* compared with model control group, there is significant difference;
Compared with model control group, middle high dose group mouse liver cell degree of necrosis mitigates, the statistically significant (p of difference<
0.05)。
According to result judgement, this product has has assistant protection function to chemical damage.
Acute toxicity test, micronucleus test, sperm malformation test, Salmonella reversion test, 30 days feeding trial results are feminine gender,
Show this product safety non-toxic.
Stability test result:Product indices are stable, meet quality standard.
Embodiment 2
Weigh in dendrobium candidum 3000g input multi-function extractors, add 30 times of amount water, be heated to boiling, insulation 3 is small
When, filter pump goes out decoction;Second is carried out to extract, plus 30 times of amount water are extracted, boiling insulation 3 hours, filter pump goes out decoction;Carry out
Third time is extracted, plus 30 times of amount water are extracted, boiling insulation 3 hours, and filter pump goes out decoction, merges No. three extract solutions and enters basin.Claim
Take in root of three-nerved spicebush 3000g, ginseng 3000g, Radix Astragali 1000g input multi-function extractors, add 10 times of amount water, be heated to boiling, protect
Temperature 2 hours, filter pump goes out decoction;Second is carried out to extract, plus 10 times of amount water are extracted, boiling insulation 2 hours, filter pump drug
Liquid;Merge extract solution twice and enter basin.Extract solution carries out being concentrated under reduced pressure into relative density 1.05 or so (60 DEG C of measurements).With malt
Dextrin and xylitol are auxiliary material, spray into medicinal extract, and boiling granulating obtains root of three-nerved spicebush iron sheet ginseng granules.
Immunologic function result of the test:
Table 6:NK cytoactives, Splenic vein hemodynamics experimental result ()
Group (g/kg) | NK cytoactives (%) | Pouring turns OD differences |
Control group | 61.0±3.0 | 0.030±0.012 |
0.67 | 61.9±3.1 | 0.050±0.009* |
1.33 | 62.1±2.9 | 0.053±0.011* |
4.0 | 62.5±2.8 | 0.061±0.008* |
Note:* compared with control group, there is significant difference
From table 6, the NK cytoactives and control group phase of the mouse of each dosage group of root of three-nerved spicebush iron sheet ginseng granules of the present invention
Than difference that there are no significant;ConA induction mouse spleen lymphocyte transformation experiment in, the OD differences of each dosage group of sample with it is right
Compared according to group, there is significant difference (p<0.05).
Table 7:DTH, spleen index and thymus index experimental result ()
Group (g/kg) | Thymus index (%) | Spleen index (%) | Swelling (auris dextra-left ear) (mg) |
Control group | 0.24±0.04 | 0.45±0.06 | 9.5±1.0 |
0.67 | 0.25±0.04 | 0.44±0.05 | 10.8±1.0 |
1.33 | 0.27±0.03 | 0.53±0.07 | 10.5±2.0 |
4.0 | 0.28±0.05 | 0.69±0.04* | 10.9±2.2 |
Note:* compared with control group, there is significant difference
From table 7, the mouse spleen index of root of three-nerved spicebush iron sheet ginseng granules high dose group of the present invention has aobvious compared with control group
Write sex differernce (p<0.05).
The phagocytosis chicken red blood cell experiment of the macrophage of table 8
Group (g/kg) | Phagocytic index | Phagocytic rate (%) |
Control group | 3.68±0.60 | 21.18±3.13 |
0.67 | 4.12±0.45 | 22.45±3.27 |
1.33 | 4.93±0.71* | 31.85±3.29* |
4.0 | 5.19±0.68* | 32.64±3.54* |
Note:* compared with control group, there is significant difference;
From table 8, the middle and high dosage group phagocytic percentage of root of three-nerved spicebush iron sheet ginseng granules of the present invention and phagocytic index are with compareing
Group is compared, and has significant difference (p<0.05).
According to result judgement, this product has strengthen immunity function.
There is assistant protection function result of the test to chemical damage:
Influence of the table 9 to mice serum ALT, AST
Group (g/kg) | ALT(U/L) | AST(U/L) |
Blank control | 68±10 | 152±36 |
Model comparison | 4401±1482 | 6621±1675 |
0.67 | 4025±1508 | 5827±1577 |
1.33 | 2867±1292* | 4338±1583* |
4.0 | 2689±1361* | 4173±1361* |
Note:* compared with model control group, there is significant difference;
From table 9, compared with blank control group, model group mouse ALT, AST are significantly raised, compared with model group, in,
ALT, AST are substantially reduced in high dose group murine liver tissue, there is significant difference (p<0.05).
Influence of the table 10 to murine liver tissue Histopathologic changes
Note:* compared with model control group, there is significant difference;
Compared with model control group, middle high dose group mouse liver cell degree of necrosis mitigates, the statistically significant (p of difference<
0.05)。
According to result judgement, this product has has assistant protection function to chemical damage.
Acute toxicity test, micronucleus test, sperm malformation test, Salmonella reversion test, 30 days feeding trial results are feminine gender,
Show this product safety non-toxic.
Stability test result:Product indices are stable, meet quality standard.
Embodiment 3
Weigh in dendrobium candidum 3000g input multi-function extractors, add 30 times of amount water, be heated to boiling, insulation 3 is small
When, filter pump goes out decoction;Second is carried out to extract, plus 30 times of amount water are extracted, boiling insulation 3 hours, filter pump goes out decoction;Carry out
Third time is extracted, plus 30 times of amount water are extracted, boiling insulation 3 hours, and filter pump goes out decoction, merges No. three extract solutions and enters basin.Claim
Take in root of three-nerved spicebush 2500g, ginseng 2500g, Radix Astragali 1000g, Chinese yam 1000g input multi-function extractors, add 10 times of amount water, heating
To seething with excitement, 2 hours are incubated, filter pump goes out decoction;Second is carried out to extract, plus 10 times of amount water are extracted, boiling insulation 2 hours, mistake
Filter pump goes out decoction;Merge extract solution twice and enter basin.Extract solution carries out being concentrated under reduced pressure into (the 60 DEG C of surveys of relative density 1.05 or so
Amount).Using maltodextrin and xylitol as auxiliary material, medicinal extract is sprayed into, boiling granulating obtains root of three-nerved spicebush iron sheet ginseng granules.
Immunologic function result of the test:
Table 11:NK cytoactives, Splenic vein hemodynamics experimental result ()
Group (g/kg) | NK cytoactives (%) | Pouring turns OD differences |
Control group | 61.0±2.9 | 0.029±0.015 |
0.67 | 62.8±2.8 | 0.051±0.010* |
1.33 | 62.7±3.0 | 0.058±0.012* |
4.0 | 63.0±2.7 | 0.062±0.010* |
Note:* compared with control group, there is significant difference
From table 11, the NK cytoactives and control group phase of the mouse of each dosage group of root of three-nerved spicebush iron sheet ginseng granules of the present invention
Than difference that there are no significant;ConA induction mouse spleen lymphocyte transformation experiment in, the OD differences of each dosage group of sample with it is right
Compared according to group, there is significant difference (p<0.05).
Table 12:DTH, spleen index and thymus index experimental result ()
Group (g/kg) | Thymus index (%) | Spleen index (%) | Swelling (auris dextra-left ear) (mg) |
Control group | 0.27±0.04 | 0.47±0.06 | 9.8±1.0 |
0.67 | 0.29±0.03 | 0.49±0.05 | 10.3±1.5 |
1.33 | 0.30±0.05 | 0.58±0.07 | 10.7±2.0 |
4.0 | 0.32±0.03 | 0.70±0.08* | 10.6±1.8 |
Note:* compared with control group, there is significant difference
From table 12, the mouse spleen index of root of three-nerved spicebush iron sheet ginseng granules high dose group of the present invention has compared with control group
Significant difference (p<0.05).
The phagocytosis chicken red blood cell experiment of the macrophage of table 13
Group (g/kg) | Phagocytic index | Phagocytic rate (%) |
Control group | 3.34±0.68 | 20.09±3.51 |
0.67 | 3.89±0.54 | 22.58±3.75 |
1.33 | 4.68±0.79* | 31.87±3.81* |
4.0 | 4.99±0.68* | 32.58±3.69* |
Note:* compared with control group, there is significant difference;
From table 13, the middle and high dosage group phagocytic percentage of root of three-nerved spicebush iron sheet ginseng granules of the present invention and phagocytic index with it is right
Compared according to group, there is significant difference (p<0.05).
According to result judgement, this product has strengthen immunity function.
There is assistant protection function result of the test to chemical damage:
Influence of the table 14 to mice serum ALT, AST
Group (g/kg) | ALT(U/L) | AST(U/L) |
Blank control | 31±14 | 137±38 |
Model comparison | 4725±1712 | 6716±1428 |
0.67 | 4084±1524 | 5407±1645 |
1.33 | 2684±1481* | 4381±1428* |
4.0 | 2571±1518* | 4173±1405* |
Note:* compared with model control group, there is significant difference;
From table 14, compared with blank control group, model group mouse ALT, AST are significantly raised, compared with model group,
ALT, AST are substantially reduced in middle and high dosage group murine liver tissue, there is significant difference (p<0.05).
Influence of the table 15 to murine liver tissue Histopathologic changes
Note:* compared with model control group, there is significant difference;
Compared with model control group, middle high dose group mouse liver cell degree of necrosis mitigates, the statistically significant (p of difference<
0.05)。
According to result judgement, this product has has assistant protection function to chemical damage.
Acute toxicity test, micronucleus test, sperm malformation test, Salmonella reversion test, 30 days feeding trial results are feminine gender,
Show this product safety non-toxic.
Stability test result:Product indices are stable, meet quality standard.
Embodiment 4
Weigh in dendrobium candidum 3000g input multi-function extractors, add 30 times of amount water, be heated to boiling, insulation 3 is small
When, filter pump goes out decoction;Second is carried out to extract, plus 30 times of amount water are extracted, boiling insulation 3 hours, filter pump goes out decoction;Carry out
Third time is extracted, plus 30 times of amount water are extracted, boiling insulation 3 hours, and filter pump goes out decoction, merges No. three extract solutions and enters basin.Claim
Take in root of three-nerved spicebush 2500g, ginseng 2500g, Radix Astragali 1000g, tuber of dwarf lilyturf 1000g input multi-function extractors, add 10 times of amount water, heating
To seething with excitement, 2 hours are incubated, filter pump goes out decoction;Second is carried out to extract, plus 10 times of amount water are extracted, boiling insulation 2 hours, mistake
Filter pump goes out decoction;Merge extract solution twice and enter basin.Extract solution carries out being concentrated under reduced pressure into (the 60 DEG C of surveys of relative density 1.05 or so
Amount).Using maltodextrin and xylitol as auxiliary material, medicinal extract is sprayed into, boiling granulating obtains root of three-nerved spicebush iron sheet ginseng granules.
Immunologic function result of the test:
Table 16:NK cytoactives, Splenic vein hemodynamics experimental result ()
Group (g/kg) | NK cytoactives (%) | Pouring turns OD differences |
Control group | 62.8±2.8 | 0.035±0.011 |
0.67 | 63.9±3.4 | 0.058±0.012* |
1.33 | 65.6±3.5 | 0.060±0.010* |
4.0 | 67.1±3.7 | 0.063±0.013* |
Note:* compared with control group, there is significant difference
From table 16, the NK cytoactives and control group phase of the mouse of each dosage group of root of three-nerved spicebush iron sheet ginseng granules of the present invention
Than difference that there are no significant;ConA induction mouse spleen lymphocyte transformation experiment in, the OD differences of each dosage group of sample with it is right
Compared according to group, there is significant difference (p<0.05).
Table 17:DTH, spleen index and thymus index experimental result ()
Group (g/kg) | Thymus index (%) | Spleen index (%) | Swelling (auris dextra-left ear) (mg) |
Control group | 0.26±0.04 | 0.40±0.05 | 9.2±1.5 |
0.67 | 0.28±0.05 | 0.41±0.06 | 10.5±1.3 |
1.33 | 0.29±0.03 | 0.50±0.07 | 10.4±2.0 |
4.0 | 0.30±0.03 | 0.67±0.04* | 10.0±1.8 |
Note:* compared with control group, there is significant difference
From table 17, the mouse spleen index of root of three-nerved spicebush iron sheet ginseng granules high dose group of the present invention has compared with control group
Significant difference (p<0.05).
The phagocytosis chicken red blood cell experiment of the macrophage of table 18
Group (g/kg) | Phagocytic index | Phagocytic rate (%) |
Control group | 3.32±0.68 | 22.51±3.68 |
0.67 | 4.02±0.51 | 23.99±3.19 |
1.33 | 4.91±0.39* | 34.18±3.52* |
4.0 | 5.28±0.61* | 35.76±3.48* |
Note:* compared with control group, there is significant difference;
From table 18, the middle and high dosage group phagocytic percentage of root of three-nerved spicebush iron sheet ginseng granules of the present invention and phagocytic index with it is right
Compared according to group, there is significant difference (p<0.05).
According to result judgement, this product has strengthen immunity function.
There is assistant protection function result of the test to chemical damage:
Influence of the table 19 to mice serum ALT, AST
Group (g/kg) | ALT(U/L) | AST(U/L) |
Blank control | 59±13 | 160±59 |
Model comparison | 4185±1381 | 6648±1498 |
0.67 | 4052±1450 | 5239±1523 |
1.33 | 2892±1518* | 4182±1497* |
4.0 | 2642±1319* | 4118±1426* |
Note:* compared with model control group, there is significant difference;
From table 19, compared with blank control group, model group mouse ALT, AST are significantly raised, compared with model group,
ALT, AST are substantially reduced in middle and high dosage group murine liver tissue, there is significant difference (p<0.05).
Influence of the table 20 to murine liver tissue Histopathologic changes
Note:* compared with model control group, there is significant difference;
Compared with model control group, middle high dose group mouse liver cell degree of necrosis mitigates, the statistically significant (p of difference<
0.05)。
According to result judgement, this product has has assistant protection function to chemical damage.
Acute toxicity test, micronucleus test, sperm malformation test, Salmonella reversion test, 30 days feeding trial results are feminine gender,
Show this product safety non-toxic.
Stability test result:Product indices are stable, meet quality standard.
Embodiment 5
Weigh in dendrobium candidum 3000g input multi-function extractors, add 30 times of amount water, be heated to boiling, insulation 3 is small
When, filter pump goes out decoction;Second is carried out to extract, plus 30 times of amount water are extracted, boiling insulation 3 hours, filter pump goes out decoction;Carry out
Third time is extracted, plus 30 times of amount water are extracted, boiling insulation 3 hours, and filter pump goes out decoction, merges No. three extract solutions and enters basin.Claim
Take in root of three-nerved spicebush 3000g, ginseng 3500g, Chinese yam 500g input multi-function extractors, add 10 times of amount water, be heated to boiling, insulation
2 hours, filter pump went out decoction;Second is carried out to extract, plus 10 times of amount water are extracted, boiling insulation 2 hours, filter pump goes out decoction;
Merge extract solution twice and enter basin.Extract solution carries out being concentrated under reduced pressure into relative density 1.05 or so (60 DEG C of measurements).Pasted with malt
Essence and xylitol are auxiliary material, spray into medicinal extract, and boiling granulating obtains root of three-nerved spicebush iron sheet ginseng granules.
Immunologic function result of the test:
Table 21:NK cytoactives, Splenic vein hemodynamics experimental result ()
Group (g/kg) | NK cytoactives (%) | Pouring turns OD differences |
Control group | 58.3±3.0 | 0.030±0.011 |
0.67 | 60.4±3.1 | 0.051±0.008* |
1.33 | 64.1±3.5 | 0.054±0.010* |
4.0 | 62.7±2.9 | 0.062±0.012* |
Note:* compared with control group, there is significant difference
From table 21, the NK cytoactives and control group phase of the mouse of each dosage group of root of three-nerved spicebush iron sheet ginseng granules of the present invention
Than difference that there are no significant;ConA induction mouse spleen lymphocyte transformation experiment in, the OD differences of each dosage group of sample with it is right
Compared according to group, there is significant difference (p<0.05).
Table 22:DTH, spleen index and thymus index experimental result ()
Group (g/kg) | Thymus index (%) | Spleen index (%) | Swelling (auris dextra-left ear) (mg) |
Control group | 0.23±0.04 | 0.40±0.06 | 8.9±1.2 |
0.67 | 0.27±0.02 | 0.44±0.07 | 10.0±1.6 |
1.33 | 0.29±0.03 | 0.52±0.08 | 9.2±2.0 |
4.0 | 0.29±0.04 | 0.68±0.05* | 9.7±1.8 |
Note:* compared with control group, there is significant difference
From table 22, the mouse spleen index of root of three-nerved spicebush iron sheet ginseng granules high dose group of the present invention has compared with control group
Significant difference (p<0.05).
The phagocytosis chicken red blood cell experiment of the macrophage of table 23
Group (g/kg) | Phagocytic index | Phagocytic rate (%) |
Control group | 3.67±0.75 | 21.82±4.21 |
0.67 | 4.19±0.51 | 23.49±4.79 |
1.33 | 4.90±0.68* | 31.85±3.92* |
4.0 | 5.31±0.56* | 33.37±3.88* |
Note:* compared with control group, there is significant difference;
From table 23, the middle and high dosage group phagocytic percentage of root of three-nerved spicebush iron sheet ginseng granules of the present invention and phagocytic index with it is right
Compared according to group, there is significant difference (p<0.05).
According to result judgement, this product has strengthen immunity function.
There is assistant protection function result of the test to chemical damage:
Influence of the table 24 to mice serum ALT, AST
Group (g/kg) | ALT(U/L) | AST(U/L) |
Blank control | 67±20 | 123±36 |
Model comparison | 4581±1212 | 6201±1619 |
0.67 | 4094±1821 | 5528±1482 |
1.33 | 2628±1480* | 4392±1602* |
4.0 | 2607±1574* | 4122±1515* |
Note:* compared with model control group, there is significant difference;
From table 24, compared with blank control group, model group mouse ALT, AST are significantly raised, compared with model group,
ALT, AST are substantially reduced in middle and high dosage group murine liver tissue, there is significant difference (p<0.05).
Influence of the table 25 to murine liver tissue Histopathologic changes
Note:* compared with model control group, there is significant difference;
Compared with model control group, middle high dose group mouse liver cell degree of necrosis mitigates, the statistically significant (p of difference<
0.05)。
According to result judgement, this product has has assistant protection function to chemical damage.
Acute toxicity test, micronucleus test, sperm malformation test, Salmonella reversion test, 30 days feeding trial results are feminine gender,
Show this product safety non-toxic.
Stability test result:Product indices are stable, meet quality standard.
Embodiment 6
Weigh in dendrobium candidum 3500g input multi-function extractors, add 30 times of amount water, be heated to boiling, insulation 3 is small
When, filter pump goes out decoction;Second is carried out to extract, plus 30 times of amount water are extracted, boiling insulation 3 hours, filter pump goes out decoction;Carry out
Third time is extracted, plus 30 times of amount water are extracted, boiling insulation 3 hours, and filter pump goes out decoction, merges No. three extract solutions and enters basin.Claim
Take in root of three-nerved spicebush 2500g, ginseng 2500g, Chinese yam 1000g, tuber of dwarf lilyturf 500g input multi-function extractors, add 10 times of amount water, heating
To seething with excitement, 2 hours are incubated, filter pump goes out decoction;Second is carried out to extract, plus 10 times of amount water are extracted, boiling insulation 2 hours, mistake
Filter pump goes out decoction;Merge extract solution twice and enter basin.Extract solution carries out being concentrated under reduced pressure into (the 60 DEG C of surveys of relative density 1.05 or so
Amount).Using maltodextrin and xylitol as auxiliary material, medicinal extract is sprayed into, boiling granulating obtains root of three-nerved spicebush iron sheet ginseng granules.
Immunologic function result of the test:
Table 26:NK cytoactives, Splenic vein hemodynamics experimental result ()
Group (g/kg) | NK cytoactives (%) | Pouring turns OD differences |
Control group | 65.1±3.0 | 0.038±0.010 |
0.67 | 64.2±3.5 | 0.055±0.012* |
1.33 | 61.8±3.7 | 0.057±0.011* |
4.0 | 63.4±3.8 | 0.066±0.015* |
Note:* compared with control group, there is significant difference
From table 26, the NK cytoactives and control group phase of the mouse of each dosage group of root of three-nerved spicebush iron sheet ginseng granules of the present invention
Than difference that there are no significant;ConA induction mouse spleen lymphocyte transformation experiment in, the OD differences of each dosage group of sample with it is right
Compared according to group, there is significant difference (p<0.05).
Table 27:DTH, spleen index and thymus index experimental result ()
Group (g/kg) | Thymus index (%) | Spleen index (%) | Swelling (auris dextra-left ear) (mg) |
Control group | 0.27±0.05 | 0.48±0.06 | 9.9±1.1 |
0.67 | 0.28±0.04 | 0.44±0.07 | 10.0±1.5 |
1.33 | 0.29±0.03 | 0.50±0.03 | 10.9±1.8 |
4.0 | 0.31±0.05 | 0.68±0.05* | 10.1±1.9 |
Note:* compared with control group, there is significant difference
From table 27, the mouse spleen index of root of three-nerved spicebush iron sheet ginseng granules high dose group of the present invention has compared with control group
Significant difference (p<0.05).
The phagocytosis chicken red blood cell experiment of the macrophage of table 28
Group (g/kg) | Phagocytic index | Phagocytic rate (%) |
Control group | 3.75±0.54 | 20.05±4.21 |
0.67 | 4.08±0.68 | 22.82±3.94 |
1.33 | 4.83±0.81* | 32.84±4.12* |
4.0 | 5.19±0.75* | 34.23±3.73* |
Note:* compared with control group, there is significant difference;
From table 28, the middle and high dosage group phagocytic percentage of root of three-nerved spicebush iron sheet ginseng granules of the present invention and phagocytic index with it is right
Compared according to group, there is significant difference (p<0.05).
According to result judgement, this product has strengthen immunity function.
There is assistant protection function result of the test to chemical damage:
Influence of the table 29 to mice serum ALT, AST
Group (g/kg) | ALT(U/L) | AST(U/L) |
Blank control | 71±11 | 101±41 |
Model comparison | 4485±1519 | 6728±1609 |
0.67 | 4354±1627 | 5347±1751 |
1.33 | 2697±1457* | 4313±1625* |
4.0 | 2602±1503* | 4201±1423* |
Note:* compared with model control group, there is significant difference;
From table 29, compared with blank control group, model group mouse ALT, AST are significantly raised, compared with model group,
ALT, AST are substantially reduced in middle and high dosage group murine liver tissue, there is significant difference (p<0.05).
Influence of the table 30 to murine liver tissue Histopathologic changes
Note:* compared with model control group, there is significant difference;
Compared with model control group, middle high dose group mouse liver cell degree of necrosis mitigates, the statistically significant (p of difference<
0.05)。
According to result judgement, this product has has assistant protection function to chemical damage.
Acute toxicity test, micronucleus test, sperm malformation test, Salmonella reversion test, 30 days feeding trial results are feminine gender,
Show this product safety non-toxic.
Stability test result:Product indices are stable, meet quality standard.
Embodiment 7
Weigh in dendrobium candidum 2000g input multi-function extractors, add 30 times of amount water, be heated to boiling, insulation 3 is small
When, filter pump goes out decoction;Second is carried out to extract, plus 30 times of amount water are extracted, boiling insulation 3 hours, filter pump goes out decoction;Carry out
Third time is extracted, plus 30 times of amount water are extracted, boiling insulation 3 hours, and filter pump goes out decoction, merges No. three extract solutions and enters basin.Claim
Take in root of three-nerved spicebush 2500g, ginseng 2500g, Radix Astragali 1000g, Chinese yam 1000g, tuber of dwarf lilyturf 1000g input multi-function extractors, add 10
Amount water, is heated to boiling again, is incubated 2 hours, filter pump goes out decoction;Second is carried out to extract, plus 10 times of amount water are extracted, boiling is protected
Temperature 2 hours, filter pump goes out decoction;Merge extract solution twice and enter basin.Extract solution carries out being concentrated under reduced pressure into the left side of relative density 1.05
Right (60 DEG C of measurements).Using maltodextrin and xylitol as auxiliary material, medicinal extract is sprayed into, boiling granulating obtains root of three-nerved spicebush iron sheet ginseng granules.
Immunologic function result of the test:
Table 31:NK cytoactives, Splenic vein hemodynamics experimental result ()
Group (g/kg) | NK cytoactives (%) | Pouring turns OD differences |
Control group | 61.5±3.2 | 0.035±0.010 |
0.67 | 63.7±3.1 | 0.054±0.013* |
1.33 | 62.0±3.0 | 0.058±0.011* |
4.0 | 64.2±3.4 | 0.062±0.010* |
Note:* compared with control group, there is significant difference
From table 31, the NK cytoactives and control group phase of the mouse of each dosage group of root of three-nerved spicebush iron sheet ginseng granules of the present invention
Than difference that there are no significant;ConA induction mouse spleen lymphocyte transformation experiment in, the OD differences of each dosage group of sample with it is right
Compared according to group, there is significant difference (p<0.05).
Table 32:DTH, spleen index and thymus index experimental result ()
Group (g/kg) | Thymus index (%) | Spleen index (%) | Swelling (auris dextra-left ear) (mg) |
Control group | 0.23±0.04 | 0.40±0.06 | 9.8±1.7 |
0.67 | 0.25±0.02 | 0.42±0.08 | 10.2±2.4 |
1.33 | 0.27±0.03 | 0.50±0.07 | 10.7±2.1 |
4.0 | 0.30±0.03 | 0.67±0.07* | 10.5±2.2 |
Note:* compared with control group, there is significant difference
From table 32, the mouse spleen index of root of three-nerved spicebush iron sheet ginseng granules high dose group of the present invention has compared with control group
Significant difference (p<0.05).
The phagocytosis chicken red blood cell experiment of the macrophage of table 33
Group (g/kg) | Phagocytic index | Phagocytic rate (%) |
Control group | 3.59±0.59 | 22.48±4.02 |
0.67 | 4.18±0.76 | 24.02±3.25 |
1.33 | 4.83±0.61* | 32.55±3.74* |
4.0 | 5.22±0.54* | 34.78±3.69* |
Note:* compared with control group, there is significant difference;
From table 33, the middle and high dosage group phagocytic percentage of root of three-nerved spicebush iron sheet ginseng granules of the present invention and phagocytic index with it is right
Compared according to group, there is significant difference (p<0.05).
According to result judgement, this product has strengthen immunity function.
There is assistant protection function result of the test to chemical damage:
Influence of the table 34 to mice serum ALT, AST
Group (g/kg) | ALT(U/L) | AST(U/L) |
Blank control | 74±19 | 139±40 |
Model comparison | 4621±1532 | 6716±1631 |
0.67 | 4243±1697 | 5638±1456 |
1.33 | 2814±1429* | 4935±1391* |
4.0 | 2628±1605* | 4365±1532* |
Note:* compared with model control group, there is significant difference;
From table 34, compared with blank control group, model group mouse ALT, AST are significantly raised, compared with model group,
ALT, AST are substantially reduced in middle and high dosage group murine liver tissue, there is significant difference (p<0.05).
Influence of the table 35 to murine liver tissue Histopathologic changes
Note:* compared with model control group, there is significant difference;
Compared with model control group, middle high dose group mouse liver cell degree of necrosis mitigates, the statistically significant (p of difference<
0.05)。
According to result judgement, this product has has assistant protection function to chemical damage.
Acute toxicity test, micronucleus test, sperm malformation test, Salmonella reversion test, 30 days feeding trial results are feminine gender,
Show this product safety non-toxic.
Stability test result:Product indices are stable, meet quality standard.
Embodiment 8
Weigh in dendrobium candidum 4000g input multi-function extractors, add 30 times of amount water, be heated to boiling, insulation 3 is small
When, filter pump goes out decoction;Second is carried out to extract, plus 30 times of amount water are extracted, boiling insulation 3 hours, filter pump goes out decoction;Carry out
Third time is extracted, plus 30 times of amount water are extracted, boiling insulation 3 hours, and filter pump goes out decoction, merges No. three extract solutions and enters basin.Claim
Take in root of three-nerved spicebush 2000g, ginseng 3000g, tuber of dwarf lilyturf 1000g input multi-function extractors, add 10 times of amount water, be heated to boiling, protect
Temperature 2 hours, filter pump goes out decoction;Second is carried out to extract, plus 10 times of amount water are extracted, boiling insulation 2 hours, filter pump drug
Liquid;Merge extract solution twice and enter basin.Extract solution carries out being concentrated under reduced pressure into relative density 1.05 or so (60 DEG C of measurements).With malt
Dextrin and xylitol are auxiliary material, spray into medicinal extract, and boiling granulating obtains root of three-nerved spicebush iron sheet ginseng granules.
Immunologic function result of the test:
Table 36:NK cytoactives, Splenic vein hemodynamics experimental result ()
Group (g/kg) | NK cytoactives (%) | Pouring turns OD differences |
Control group | 63.6±3.6 | 0.033±0.015 |
0.67 | 64.3±3.3 | 0.057±0.012* |
1.33 | 62.3±3.5 | 0.059±0.011* |
4.0 | 65.1±3.2 | 0.060±0.010* |
Note:* compared with control group, there is significant difference
From table 36, the NK cytoactives and control group phase of the mouse of each dosage group of root of three-nerved spicebush iron sheet ginseng granules of the present invention
Than difference that there are no significant;ConA induction mouse spleen lymphocyte transformation experiment in, the OD differences of each dosage group of sample with it is right
Compared according to group, there is significant difference (p<0.05).
Table 37:DTH, spleen index and thymus index experimental result ()
Group (g/kg) | Thymus index (%) | Spleen index (%) | Swelling (auris dextra-left ear) (mg) |
Control group | 0.22±0.03 | 0.45±0.08 | 9.9±1.8 |
0.67 | 0.24±0.04 | 0.43±0.06 | 10.3±1.4 |
1.33 | 0.27±0.05 | 0.52±0.06 | 10.7±1.8 |
4.0 | 0.31±0.04 | 0.72±0.07* | 10.0±1.9 |
Note:* compared with control group, there is significant difference
From table 37, the mouse spleen index of root of three-nerved spicebush iron sheet ginseng granules high dose group of the present invention has compared with control group
Significant difference (p<0.05).
The phagocytosis chicken red blood cell experiment of the macrophage of table 38
Group (g/kg) | Phagocytic index | Phagocytic rate (%) |
Control group | 3.68±0.56 | 21.42±4.12 |
0.67 | 4.22±0.43 | 23.38±3.81 |
1.33 | 4.61±0.71* | 33.48±3.94* |
4.0 | 5.31±0.64* | 34.14±3.75* |
Note:* compared with control group, there is significant difference;
From table 38, the middle and high dosage group phagocytic percentage of root of three-nerved spicebush iron sheet ginseng granules of the present invention and phagocytic index with it is right
Compared according to group, there is significant difference (p<0.05).
According to result judgement, this product has strengthen immunity function.
There is assistant protection function result of the test to chemical damage:
Influence of the table 39 to mice serum ALT, AST
Group (g/kg) | ALT(U/L) | AST(U/L) |
Blank control | 73±21 | 138±53 |
Model comparison | 4462±1361 | 6735±1439 |
0.67 | 4036±1436 | 5368±1395 |
1.33 | 2834±1532* | 4395±1627* |
4.0 | 2692±1543* | 4168±1386* |
Note:* compared with model control group, there is significant difference;
From table 39, compared with blank control group, model group mouse ALT, AST are significantly raised, compared with model group,
ALT, AST are substantially reduced in middle and high dosage group murine liver tissue, there is significant difference (p<0.05).
Influence of the table 40 to murine liver tissue Histopathologic changes
Note:* compared with model control group, there is significant difference;
Compared with model control group, middle high dose group mouse liver cell degree of necrosis mitigates, the statistically significant (p of difference<
0.05)。
According to result judgement, this product has has assistant protection function to chemical damage.
Acute toxicity test, micronucleus test, sperm malformation test, Salmonella reversion test, 30 days feeding trial results are feminine gender,
Show this product safety non-toxic.
Stability test result:Product indices are stable, meet quality standard.
The root of three-nerved spicebush, ginseng heat, dendrobium candidum are cold in nature, and three's compatibility is coordinated, reinforced vital energy as main ingredient, negative and positive, beneficial stomach shield
Liver.The complete square tonifying Qi effect after the Radix Astragali that adds more preferably, was adapted to deficiency of vital energy person and taken as ministerial drug the Radix Astragali, Chinese yam, the tuber of dwarf lilyturf;Add Chinese yam
Complete square tonifying spleen effect is notable afterwards, and suitable person with Spleen Deficiency is taken;Add complete square enriching yin effect after the tuber of dwarf lilyturf to strengthen, be adapted to deficiency of Yin person and take.
Claims (8)
1. a kind of root of three-nerved spicebush iron sheet ginseng granules agent, it is characterised in that composed of the following components:Dendrobium candidum 10-40 parts by weight, crow
Medicine 10-40 parts by weight, ginseng 10-40 parts by weight.
2. a kind of root of three-nerved spicebush iron sheet ginseng granules agent according to claim 1, it is characterised in that also including Radix Astragali 1-10 weight
Part.
3. a kind of root of three-nerved spicebush iron sheet ginseng granules agent according to claim 2, it is characterised in that also including Chinese yam 1-10 weight
Part.
4. a kind of root of three-nerved spicebush iron sheet ginseng granules agent according to claim 2, it is characterised in that also including tuber of dwarf lilyturf 1-10 weight
Part.
5. a kind of root of three-nerved spicebush iron sheet ginseng granules agent according to claim 1, it is characterised in that also including Chinese yam 1-10 weight
Part.
6. a kind of root of three-nerved spicebush iron sheet ginseng granules agent according to claim 5, it is characterised in that also including tuber of dwarf lilyturf 1-10 weight
Part.
7. a kind of root of three-nerved spicebush iron sheet ginseng granules agent according to claim 6, it is characterised in that also including Radix Astragali 1-10 weight
Part.
8. a kind of root of three-nerved spicebush iron sheet ginseng granules agent according to claim 1, it is characterised in that also including tuber of dwarf lilyturf 1-10 weight
Part.
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CN107595937B (en) * | 2017-11-03 | 2021-02-23 | 浙江中医药大学 | Combined spicebush root composition with inflammatory bowel disease antagonism and application thereof |
CN108142768A (en) * | 2017-11-17 | 2018-06-12 | 浙江科技学院 | A kind of solid particle and its production method based on combined spicebush leaf |
CN108419977A (en) * | 2018-03-12 | 2018-08-21 | 黑龙江军门现代农业发展有限公司 | A kind of Silybum Marianum Gaertn Seed Oil solid beverage of relieving alcoholism and protecting liver and preparation method thereof |
CN108645802B (en) * | 2018-05-16 | 2020-01-03 | 安徽农业大学 | Detection method for content of total alkaloids in dendrobium officinale |
CN112021566A (en) * | 2020-08-24 | 2020-12-04 | 浙江红石梁集团天台山乌药有限公司 | Dendrobium officinale, American ginseng and radix ophiopogonis granules |
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Denomination of invention: Black medicine Tiepi ginseng granule Effective date of registration: 20210713 Granted publication date: 20171031 Pledgee: Zhejiang Rural Commercial Bank branch Limited by Share Ltd. yin'an Tiantai Pledgor: ZHEJIANG HONGSHILIANG GROUP TIANTAISHAN SPICEBUSH ROOT Co.,Ltd. Registration number: Y2021330000868 |