CN103890167A - 自多潜能干细胞有效诱导定形内胚层 - Google Patents

自多潜能干细胞有效诱导定形内胚层 Download PDF

Info

Publication number
CN103890167A
CN103890167A CN201280040881.9A CN201280040881A CN103890167A CN 103890167 A CN103890167 A CN 103890167A CN 201280040881 A CN201280040881 A CN 201280040881A CN 103890167 A CN103890167 A CN 103890167A
Authority
CN
China
Prior art keywords
cell
chir
concentration
stem cell
activin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201280040881.9A
Other languages
English (en)
Chinese (zh)
Inventor
N.富纳
K.赫斯
J.伊克伯格
H.塞姆布
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Celatis Co ltd
Novo Nordisk AS
Original Assignee
Celatis Co ltd
Novo Nordisk AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Celatis Co ltd, Novo Nordisk AS filed Critical Celatis Co ltd
Priority to CN201810066827.0A priority Critical patent/CN108220224A/zh
Publication of CN103890167A publication Critical patent/CN103890167A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C12N5/0678—Stem cells; Progenitor cells; Precursor cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Transplantation (AREA)
  • Diabetes (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Hematology (AREA)
  • Endocrinology (AREA)
  • Emergency Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Obesity (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
CN201280040881.9A 2011-06-21 2012-06-21 自多潜能干细胞有效诱导定形内胚层 Pending CN103890167A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810066827.0A CN108220224A (zh) 2011-06-21 2012-06-21 自多潜能干细胞有效诱导定形内胚层

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP11170713.9 2011-06-21
EP11170713 2011-06-21
US201161501351P 2011-06-27 2011-06-27
US61/501351 2011-06-27
PCT/EP2012/062013 WO2012175633A1 (en) 2011-06-21 2012-06-21 Efficient induction of definitive endoderm from pluripotent stem cells

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN201810066827.0A Division CN108220224A (zh) 2011-06-21 2012-06-21 自多潜能干细胞有效诱导定形内胚层

Publications (1)

Publication Number Publication Date
CN103890167A true CN103890167A (zh) 2014-06-25

Family

ID=47422055

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201280040881.9A Pending CN103890167A (zh) 2011-06-21 2012-06-21 自多潜能干细胞有效诱导定形内胚层
CN201810066827.0A Pending CN108220224A (zh) 2011-06-21 2012-06-21 自多潜能干细胞有效诱导定形内胚层

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN201810066827.0A Pending CN108220224A (zh) 2011-06-21 2012-06-21 自多潜能干细胞有效诱导定形内胚层

Country Status (6)

Country Link
US (3) US20140234963A1 (enExample)
EP (1) EP2723852B1 (enExample)
JP (1) JP6312591B2 (enExample)
CN (2) CN103890167A (enExample)
ES (1) ES2902650T3 (enExample)
WO (1) WO2012175633A1 (enExample)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2902650T3 (es) 2011-06-21 2022-03-29 Novo Nordisk As Inducción eficiente de endodermo definitivo a partir de células madre pluripotentes
JP6470687B2 (ja) 2012-09-03 2019-02-13 ノヴォ ノルディスク アー／エス 小分子を用いた多能性幹細胞からの膵臓内胚葉の作製
GB201317869D0 (en) * 2013-10-09 2013-11-20 Cambridge Entpr Ltd In vitro production of foregut stem cells
ES2967942T3 (es) 2016-02-24 2024-05-06 Novo Nordisk As Generación de células beta funcionales a partir de progenitoras endocrinas derivadas de células madre pluripotentes humanas
JP2020532992A (ja) * 2017-09-07 2020-11-19 ステモニックス インコーポレイティド 胚体内胚葉へのヒト幹細胞の効率的な分化
CN111108190B (zh) 2017-09-11 2024-06-21 诺和诺德股份有限公司 在体外从干细胞衍生的nkx6.1和c-肽共表达细胞的富集
WO2019217875A1 (en) 2018-05-11 2019-11-14 Cornell University Method of differentiation of human pluripotent stem cells to monohormonal cells
CA3110932A1 (en) 2018-08-30 2020-03-05 Novo Nordisk A/S Generation of functional beta cells from human pluripotent stem cell-derived endocrine progenitors
JP7755487B2 (ja) 2019-04-08 2025-10-16 ノヴォ ノルディスク アー／エス 幹細胞由来の胚体内胚葉からの膵臓内胚葉の生成
EP4061929A1 (en) 2019-11-22 2022-09-28 Novo Nordisk A/S Spin-aggregated neural microspheres and the application thereof
US20230313145A1 (en) 2020-08-28 2023-10-05 Novo Nordisk A/S Method for screening in vitro population of stem cell derived beta like cells and novel markers thereof
WO2024008810A1 (en) 2022-07-06 2024-01-11 Novo Nordisk A/S Differentiation of stem cells to pancreatic endocrine cells
WO2024151541A1 (en) 2023-01-09 2024-07-18 Sana Biotechnology, Inc. Type-1 diabetes autoimmune mouse
WO2024243236A2 (en) 2023-05-22 2024-11-28 Sana Biotechnology, Inc. Methods of delivery of islet cells and related methods
WO2025093467A1 (en) 2023-10-31 2025-05-08 Novo Nordisk A/S Methods for preventing unwanted cells arising during differentiation of human pluripotent stem cells
EP4491712A1 (en) 2024-03-20 2025-01-15 Novo Nordisk A/S Method for detecting contaminant cells in a stem cell derived cell population

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009027654A1 (en) * 2007-08-24 2009-03-05 University Court Of The University Of Edinburgh Regionalised endoderm cells and uses thereof
US20090298178A1 (en) * 2008-06-03 2009-12-03 D Amour Kevin Allen Growth factors for production of definitive endoderm
WO2009154606A1 (en) * 2008-06-03 2009-12-23 Cythera, Inc. Growth factors for production of definitive endoderm
CN101684454A (zh) * 2008-09-25 2010-03-31 中国科学院上海生命科学研究院 一种定型内胚层细胞的制备和分离方法

Family Cites Families (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5523226A (en) * 1993-05-14 1996-06-04 Biotechnology Research And Development Corp. Transgenic swine compositions and methods
JP4533534B2 (ja) 1998-06-19 2010-09-01 ノバルティス バクシンズ アンド ダイアグノスティックス，インコーポレーテッド グリコーゲンシンターゼキナーゼ３のインヒビター
EP1393066A4 (en) 2001-05-15 2006-01-25 Rappaport Family Inst For Res INSULIN-PRODUCING CELLS DERIVED FROM HUMAN EMBRYONIC STEM CELLS
GB2399823B (en) 2001-12-07 2006-02-15 Geron Corp Islet cells from primate pluripotent stem cells
GB2398795A (en) 2001-12-28 2004-09-01 Cellartis Ab A method for the establishment of a pluripotent human blastocyst-derived stem cell line
US7625753B2 (en) 2003-12-23 2009-12-01 Cythera, Inc. Expansion of definitive endoderm cells
KR101221302B1 (ko) 2003-12-23 2013-01-11 비아싸이트, 인크. 완전 내배엽
ES2751095T3 (es) 2004-04-27 2020-03-30 Viacyte Inc Endodermo que expresa pdx1
WO2007042225A2 (en) 2005-10-07 2007-04-19 Cellartis Ab A method for obtaining a xeno-free hbs cell line
CA2644468C (en) 2006-03-02 2022-02-01 Cythera, Inc. Endocrine precursor cells, pancreatic hormone-expressing cells and methods of production
GB0615327D0 (en) 2006-03-30 2006-09-13 Univ Edinburgh Culture medium containing kinase inhibitors and uses thereof
EP2021462B1 (en) 2006-04-28 2019-01-09 Lifescan, Inc. Differentiation of human embryonic stem cells
US8741643B2 (en) * 2006-04-28 2014-06-03 Lifescan, Inc. Differentiation of pluripotent stem cells to definitive endoderm lineage
CA2654196A1 (en) 2006-06-02 2007-12-13 University Of Georgia Research Foundation, Inc. Pancreatic and liver endoderm cells and tissue by differentiation of definitive endoderm cells obtained from human embryonic stems
AU2008211103B2 (en) * 2007-01-30 2014-05-08 University Of Georgia Research Foundation, Inc. Early mesoderm cells, a stable population of mesendoderm cells that has utility for generation of endoderm and mesoderm lineages and multipotent migratory cells (MMC)
JP5646990B2 (ja) 2007-04-23 2014-12-24 ストワーズ インスティテュート フォー メディカル リサーチ 幹細胞自己複製のための方法及び組成物
US9080145B2 (en) * 2007-07-01 2015-07-14 Lifescan Corporation Single pluripotent stem cell culture
BRPI0814425A2 (pt) 2007-07-18 2014-10-21 Lifescan Inc Diferenciação de células-tronco embrionárias humanas
SG183067A1 (en) 2007-07-20 2012-08-30 Cellartis Ab A novel population of hepatocytes derived via definitive endoderm (de-hep) from human blastocysts derived stem cells
JP6087043B2 (ja) 2007-07-31 2017-03-01 ライフスキャン・インコーポレイテッドＬｉｆｅｓｃａｎ，Ｉｎｃ． ヒトフィーダー細胞を用いた多能性幹細胞の分化
CA3114827C (en) 2007-07-31 2023-09-05 Lifescan, Inc. Differentiation of human embryonic stem cells to pancreatic endocrine
US7939322B2 (en) 2008-04-24 2011-05-10 Centocor Ortho Biotech Inc. Cells expressing pluripotency markers and expressing markers characteristic of the definitive endoderm
US8623648B2 (en) 2008-04-24 2014-01-07 Janssen Biotech, Inc. Treatment of pluripotent cells
EP2297298A4 (en) 2008-05-09 2011-10-05 Vistagen Therapeutics Inc PANCREATIC ENDOCRINE PROGENITOR CELLS FROM PLURIPOTENT STEM CELLS
MX2011000123A (es) 2008-06-30 2011-02-25 Centocor Ortho Biotech Inc Diferenciacion de las celulas madre pluripotentes.
AU2009267137A1 (en) 2008-06-30 2010-01-07 Centocor Ortho Biotech Inc. Differentiation of pluripotent stem cells
US20100028307A1 (en) 2008-07-31 2010-02-04 O'neil John J Pluripotent stem cell differentiation
US8278105B2 (en) * 2008-09-09 2012-10-02 University Of Southern California Induction, propagation and isolation of liver progenitor cells
MX2011004563A (es) 2008-10-31 2011-06-01 Centocor Ortho Biotech Inc Diferenciacion de celulas madre embrionarias humanas al linaje endocrino pancreatico.
MX2011004565A (es) 2008-10-31 2011-07-28 Centocor Ortho Biotech Inc Diferenciacion de celulas madre embrionarias humanas al linaje endocrino pancreatico.
EP3363444B1 (en) 2008-11-14 2022-09-14 ViaCyte, Inc. Encapsulation of pancreatic cells derived from human pluripotent stem cells
US8507274B2 (en) 2009-02-06 2013-08-13 President And Fellows Of Harvard College Compositions and methods for promoting the generation of definitive endoderm
WO2010124142A2 (en) 2009-04-22 2010-10-28 Cythera, Inc. Cell compositions derived from dedifferentiated reprogrammed cells
KR20120047866A (ko) 2009-05-27 2012-05-14 더 솔크 인스티튜트 포 바이올로지칼 스터디즈 유전자 교정된 무질환 유도 만능 줄기 세포의 생성
EP2456862A4 (en) 2009-07-20 2013-02-27 Janssen Biotech Inc DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS
KR101785626B1 (ko) * 2009-07-20 2017-10-16 얀센 바이오테크 인코포레이티드 인간 배아 줄기 세포의 분화
BR112012017761A2 (pt) 2009-12-23 2015-09-15 Centocor Ortho Biotech Inc diferenciação das células-tronco embrionárias humanas
PH12012501254A1 (en) 2009-12-23 2012-11-05 Janssen Biotech Inc Differentiation of human embryonic stem cells
WO2011081222A1 (ja) 2009-12-29 2011-07-07 武田薬品工業株式会社 膵ホルモン産生細胞の製造法
US9969981B2 (en) 2010-03-01 2018-05-15 Janssen Biotech, Inc. Methods for purifying cells derived from pluripotent stem cells
US9719068B2 (en) 2010-05-06 2017-08-01 Children's Hospital Medical Center Methods and systems for converting precursor cells into intestinal tissues through directed differentiation
RU2663339C1 (ru) 2010-05-12 2018-08-03 Янссен Байотек, Инк. Дифференцирование эмбриональных стволовых клеток человека
US9085757B2 (en) 2010-06-17 2015-07-21 Regents Of The University Of Minnesota Production of insulin producing cells
KR20190027969A (ko) 2010-08-12 2019-03-15 얀센 바이오테크 인코포레이티드 췌장 내분비 전구 세포를 이용한 당뇨병의 치료
KR101836850B1 (ko) 2010-08-31 2018-03-09 얀센 바이오테크 인코포레이티드 인간 배아 줄기 세포의 분화
CA2809300A1 (en) 2010-08-31 2012-03-08 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
US9062292B2 (en) 2010-09-13 2015-06-23 Enzo Life Sciences Inc. Mutant T7 polymerases
WO2012070014A2 (en) 2010-11-26 2012-05-31 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Identification of novel cell surface markers for pancreatic progenitor cells and definite endodermal cells
CN103354835B (zh) 2010-12-08 2016-03-02 韦尔赛特公司 抑制人多能干细胞生长的试剂和方法
JP5922147B2 (ja) 2010-12-15 2016-05-24 カディマステム リミテッド 多能性幹細胞に由来するインスリン産生細胞
ES2902650T3 (es) 2011-06-21 2022-03-29 Novo Nordisk As Inducción eficiente de endodermo definitivo a partir de células madre pluripotentes

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009027654A1 (en) * 2007-08-24 2009-03-05 University Court Of The University Of Edinburgh Regionalised endoderm cells and uses thereof
US20090298178A1 (en) * 2008-06-03 2009-12-03 D Amour Kevin Allen Growth factors for production of definitive endoderm
WO2009154606A1 (en) * 2008-06-03 2009-12-23 Cythera, Inc. Growth factors for production of definitive endoderm
CN101684454A (zh) * 2008-09-25 2010-03-31 中国科学院上海生命科学研究院 一种定型内胚层细胞的制备和分离方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
孙彦洵 等: "利用胚胎干细胞分化的拟胚体研究小鼠早期胚胎发育过程", 《生物技术通讯》 *

Also Published As

Publication number Publication date
US10487313B2 (en) 2019-11-26
ES2902650T3 (es) 2022-03-29
CN108220224A (zh) 2018-06-29
US20180002668A1 (en) 2018-01-04
EP2723852B1 (en) 2021-11-17
JP6312591B2 (ja) 2018-04-18
US20140234963A1 (en) 2014-08-21
EP2723852A1 (en) 2014-04-30
JP2014519832A (ja) 2014-08-21
US20200190477A1 (en) 2020-06-18
WO2012175633A1 (en) 2012-12-27

Similar Documents

Publication Publication Date Title
CN103890167A (zh) 自多潜能干细胞有效诱导定形内胚层
CN102083965B (zh) 多潜能细胞
JP6966583B2 (ja) 多能性幹細胞の膵臓内胚葉細胞（ＰＥＣ）および内分泌細胞へのｉｎ ｖｉｔｒｏ分化
RU2650813C2 (ru) Использование лигандов эпинефрина для дифференцирования клеток панкреатической эндодермы
RU2668814C2 (ru) Способы продуцирования клеток дефинитивной энтодермы и панкреатической энтодермы
CN105358680B (zh) 用于悬浮培养内胚层祖细胞的方法和组合物
RU2668798C2 (ru) Способы in vitro пошаговой дифференцировки полюрипотентных клеток
Iacovitti et al. A protocol for the differentiation of human embryonic stem cells into dopaminergic neurons using only chemically defined human additives: studies in vitro and in vivo
CN105793414B (zh) 前肠干细胞的活体外生产
US8278105B2 (en) Induction, propagation and isolation of liver progenitor cells
CN102105580B (zh) 多潜能细胞的处理
JP6449829B2 (ja) ヒト胚性幹細胞の分化
KR102403165B1 (ko) 만능성 줄기 세포의 장의 중장 내배엽 세포로의 분화
Meng et al. The differentiation and isolation of mouse embryonic stem cells toward hepatocytes using galactose-carrying substrata
CN113151147A (zh) 功能性肝实质细胞及其制备方法
WO2024047188A1 (en) Extraembryonic mesoderm cells

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C53 Correction of patent of invention or patent application
CB02 Change of applicant information

Address after: Denmark bagsvaerd

Applicant after: Novo Nordisk AS

Applicant after: CELLECTIS S.A.

Address before: Denmark bagsvaerd

Applicant before: Novo Nordisk AS

Applicant before: Cellartis AB

COR Change of bibliographic data

Free format text: CORRECT: ADDRESS; FROM:

Free format text: CORRECT: APPLICANT; FROM: CELLARTIS AB TO: TAKARA BIOTECHNOLOGY EUROPE AG

RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20140625