CN103548654B - Method for breeding virus-free micro-potatoes by integrated net house - Google Patents
Method for breeding virus-free micro-potatoes by integrated net house Download PDFInfo
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a method for breeding virus-free micro-potatoes by an integrated net house and belongs to the technical field of potato breeding. The invention adopts the technical scheme that the method comprises the following process steps of: 1, selecting a breeding facility; 2, carrying out seedling exercising, transplantation and fixed planting; 3, preparing nutrient solution and applying a fertilizer; 4, managing the temperature of the net house; 5, carrying out seedling pressure, vermiculite culture and tip shearing; 6, carrying out ozone air sterilization and nutrient solution disinfection; 7, carrying out carbon dioxide fertilization; 8, harvesting the micro-potatoes. According to the invention, seedling exercising, field planting and the subsequent growing process in the micro-potato production are guaranteed not to be infected by viruses which use poison insects as vectors; the ventilation condition of a substrate on a culture bed is improved; quality of seed potatoes of the micro-potatoes is improved; tuberization is uniform; meanwhile, pollution to the environment is avoided; under the condition of the net house, the virus-free micro-potatoes are cultured by utilizing the soilless substrate and micro tubers with weights of 5 to 15g are bred. The virus-free seed micro-potatoes provide an important mode which solves the problem of seed nature degeneration caused by virus infection and is used for the production of virus-free seed potatoes.
Description
Technical field
The present invention relates to a kind of integrated net canopy potato detoxicating micro potato mating system, belong to potato raising technology field.
Background technology
At present, potato detoxicating micro potato is bred two kinds of conventional methods, and one is that group training seedling completes micro potato knot potato time of infertility in bottle, whole process is all indoor the completing of group training, and the micro potato that this mode is born is generally in 0.5～2g left and right, individual little, skin is thin, is difficult for storage.Another kind method is utilization group training seedling, produce taking vermiculite as the indoor ground connection cultivation of matrix canopy bed, moisture supersaturation in ground connection cultivation bed Chang Yinwei matrix and affect the breathing of root system, cause root system to rot, affect plant strain growth, in production process, need repeatedly to come with agricultural chemicals the generation of disease and pest control, ventilation solves the deficiency of carbonic acid gas, plant, in lacking raw material, is manufactured nutrient few within a certain period of time, and micro potato is differed in size.When micro potato is gathered, do not kill seedling, micro potato physiological age is increased, increased the probability of Virus entry.
Summary of the invention
The object of this invention is to provide a kind of integrated net canopy potato detoxicating micro potato mating system, guarantee the virus infection that in hardening, field planting and subsequent the process of growth in micro potato production, tape kissing bug is not amboceptor, improve the aeration condition of the upper matrix of cultivation bed, micro potato potato seed quality improves, knot potato is even, avoid the pollution of environment simultaneously, solve the problems referred to above that background technology exists.
Technical scheme of the present invention is: a kind of integrated net canopy potato detoxicating micro potato mating system, comprises following processing step: 1. breed facility and select; 2. hardening, transplanting and field planting; 3. nutrient solution prescription and fertilising; 4. net canopy temperature treatment; 5. press seedling, train vermiculite and cut point; 6. ozone-air sterilization and nutrient solution sterilization; 7. carbon dioxide fertilization; 8. micro potato is gathered.
Each processing step concrete operations are as follows:
1. breeding facility selects
The facility of producing toxicity micro potato is arch-shaped steel frame construction plastic tunnel, is called for short net canopy, and fly net is set, and hangs polychrome dielectric fluke plate, and ozone for preventing and controlling machine and Rui Te A type carbonic acid gas growing aid are set;
2. hardening, transplanting and field planting
Hardening: hardening is the environment after tissue-culture container seedling adapts to transplant and carries out autotrophy, the process of progressively taking exercise and adapting to, is the key link comparatively in potato detoxicating tissue culture technology; Detect and be accredited as virus-free bottle seedling through enzyme linked immunological virus, through expansion numerous switching 20～25d seedling age in blake bottle, high 5～8cm, the bottle seedling of 5～6 blades, moves on in net canopy, is placed on furrow bottle spacing 5cm; Field planting is opened sealed membrane in first 3～5 days, and tender seedling is contacted with the external world gradually, improves adaptive capacity;
Transplant: first make the unsettled liftoff cultivation bed with ground isolation, in net canopy, build apart from 3 blocks of common bricks under the 20cm(asbestos shingle of ground with brick), two blocks of asbestos shingles of wide 140cm(are wide) cultivation bed, at the bottom of making bed of asbestos shingle, bedside edge is used brick limit, bed height 15cm; In bed, fill the thick vermiculite matrix through high-temperature roasting of 7-9cm, do not execute any base fertilizer, after leveling, plant before seedling, with 800 times of liquid of thiophanate methyl, ten thousand times of liquid of 70% sharp victory WS (Diacloden) 3-4, ground sterilization in net canopy; Adopt unsettled liftoff cultivation bed, cultivation seedling initial stage temperature is higher 2～3 DEG C than ground connection cultivation bed tempertaure; Higher seedling bed temperature during this is conducive to slow seedling, favourable to the survival rate of transplanted seedling; The bed body of unsettled liftoff cultivation bed and ground isolation, unnecessary moisture oozes out from bottom, and cultivation bed aeration condition is good, is conducive to the growth of root; Pathogen that canopy carries by pin and trouser legs when inside and outside infects marginal rice shoot can also to avoid manager to go out to network;
Transplant planting: when the stable field planting during by 4～6 DEG C of ground temperature in net canopy, first from blake bottle, detoxic seedling is taken out before transplanting, wash away root medium with clear water; Adopt spacing in the rows × line-spacing=5cm × 7.5cm, i.e. 250 strains/m
2, single cave individual plant, presss from both sides the root of medium and seedling with tweezers when cuttage, finger presses detoxic seedling base portion, and seedling waters after being combined with matrix and closely planting seedling; The outer covered with sunshade net of net canopy, appropriateness is sheltered from heat or light and is delayed Miao Yizhou, prevents that direct sunlight shines seedling, after transplanted seedling grows new root after one week, progressively removes sunshade net; Soil humidity is controlled at 70%～80% left and right; After cultivation seedling, after 7～10 days 80% seedling rootings, start spray nutritious liquor;
3. nutrient solution prescription and fertilising
After field planting 7～21 days, adopt formula mg/kg: nitrate of lime 100, potassium nitrate 1034, magnesium sulfate 490, potassium dihydrogen phosphate 348, ammonium sulfate 170, calcium chloride 150, EDTA-Na(EDTA-sodium) 37.25, ferrous sulfate 27.85, copper sulphate 100; Filled with and execute one time of nutrition liquid every 5 days.
After the present embodiment field planting 3 weeks, impose fertilizer type and concentration and 667m
2fertilizer amount (pouring)
After field planting, in 21～75 days, impose every 10 days blade faces 600 times of tubers (0-36-24+TE+FV) " promise Cui Feng " the potassium dihydrogen phosphate special-purpose foliage fertilizers that Zhejiang Ai Pu development in agricultural science and technology Co., Ltd produces;
4. net canopy temperature treatment
In net canopy, detoxic seedling is transplanted latter one week interior air temperature control at 25～30 DEG C, exceedes 30 DEG C of coolings of will leaking informaton; Seedling Stage is that before the 6th leaf or the 8th leaf launch, temperature is controlled between 20～25 DEG C, is beneficial to potato nutritional organ and builds; Potato tubers forms phase temperature and is controlled between 15～20 DEG C, and at expanding stage, potato needs higher day temperature, 18～20 DEG C, 12～14 DEG C of lower nocturnal temperatures; Alternating temperature management is key factor during micro potato is produced, and when temperature is high, strengthen ventilating management, adopts if desired forced ventilation measure, guarantees that temperature is in optimum scope;
5. press seedling, train vermiculite and cut point
In the time that rice shoot grows to 10 leaves, press seedling,, at 2～3 leaf places, top of seedling seedling is overwhelmed on medium with forefinger, cover matrix 2cm above, bed surface exposes 2～3 leaves; In the time that reaching 7 leaf, 80% plant leaf carries out training vermiculite the 1st time, training vermiculite 3～4 times in vegetative period, and thickness reaches 5～6cm, each training vermiculite is only trained two stipes of base portion, when field planting 7 weeks, cut off apical growing point, making axillary bud sprouting is side shoot, and seedling growing height is controlled at 25～30cm; Press seedling, train vermiculite and cut point and can increase the generation of stolon, reduce plant height, the apical dominance of plant is suppressed, the Nutrition flows stem tuber that blade is manufactured, and then improve the knot potato rate of micro potato, increase micro potato stem tuber weight;
6. ozone-air sterilization and nutrient solution sterilization
Ozone is a kind of very strong oxidizing gas that has, and can sterilize to air sterilization; Ozone for preventing and controlling machine is made up of ozone main frame, air inlet pipe, anemostat, controller three parts, and wherein ozone for preventing and controlling machine is made up of ozone generation body, air pump etc.; Ozone for preventing and controlling machine hangs on net canopy central authorities skeleton, the exhaust outlet of two anemostat one end and ozone main frame joins, carry out electrion by the air in suction machine and make air ozonisation, the air of ozonisation is exported by air pump in machine again, and spreads out equably along the plastics trunnion that is laid on net canopy space; Ozone gas can be prevented and treated the generation of aeroborne disease, better to control efficiency such as potato morning, late blights; After ozone is water-soluble, will become a kind of strong oxidizer, can produce a kind of active ion, be connected with nutrient solution supply system, active ion has killing action to the pathogen in cultivation matrix; The ozone that ozone for preventing and controlling machine produces also contains nitrogen oxide simultaneously, and nitrogen oxide can be used as the required nitrogenous fertilizer of potato virus-free plantlet and uses; Service time: after the slow seedling of field planting, bring into use, the morning 8:00～9:00, afternoon 17:00～18:00, programmable timer timing setting, controls the duty of ozone main frame, micro potato is gathered and is stopped the last week;
7. carbon dioxide fertilization
After the slow seedling of detoxic seedling field planting, blade grows to and approaches into age when size, and field planting, after 3 weeks, starts to enrich CO 2 gas fertilizer; Raw material is carbonic hydroammonium, and generation gas apparatus used is auspicious special A type carbonic acid gas growing aid; When every morning 8:00～11:00, before ventilation, stop using;
8. micro potato is gathered
When plant lower blade reaches 1/3rd flavescence, can gather, be controlled at 75-80 days results vegetative period, guarantee definitely young potato seed physiological age; Before gathering, Du Pont can kill to such an extent that seedling is killed in 3,000 (active ingredient: 46.1% Kocide SD, formulation: water dispersion granule) 1500 times of sprayings; Gather respectively by micro potato grade size, seed coat is fresh and tender, prevents broken skin phenomenon; Micro potato water content while gathering is large, is placed on airing 7～10d in ventilating and cooling place after gathering; Pack seed packet into, post label, put cryopreservation under 2～4 DEG C of conditions in storage.
Described net canopy arranges fly net, selects the high-quality fly net in 60 order apertures to hide shed, is fixed on skeleton, obturages and can not leave gap, extends to ground from lower air port; Double-deck fly net (buffered gate) is established in import and export, and fly net will be strained; It is tight that fly net is wanted, ground connection place's compacting, and seam crossing is sewed up; Around solarium, within the scope of 10m, can not there is other may become potato disease insect pest source of infection and maybe may become aphid host's plant.
Described polychrome dielectric fluke plate, is made up of high voltage source, substrate, dielectric coat, yellow, blue wear-resistant protective layer; The discrepancy place of opening the door inner side at fly net head-on hangs a fluke plate, utilizes adult love look, adopts colour table to add dielectric adsorption technology the adult of insect is attracted to and on plate, cements and kill; In blink in administrative staff pass in and out facility, the infection insect entering with people runs into the special tone of colour table to stimulate, and can be killed by dielectric fluke plate towards colour table motion by the light of nature; Every 20m in fly net
2put a fluke plate, fluke plate is suspended on to rice shoot top, bottom exceeds plant l0cm～15cm, traps and kills the adult that slips through the net, and fluke plate face is clean with wet cloth erasing in time.
The technology of the present invention feature:
1. fly net and import and export are set establishes double-deck fly net (buffered gate) and can intercept infection insect and enter in canopy, guaranteeing the virus infection that in hardening, field planting and subsequent the process of growth in micro potato production, tape kissing bug is not amboceptor, is the key that ensures detoxification efficiency.
2. hanging polychrome dielectric fluke plate can avoid passing in and out as administrative staff the insect entering with people in the blink in facility.
3. unsettled liftoff cultivation bed allows unnecessary moisture ooze out from bottom, has improved the aeration condition of the upper matrix of cultivation bed, is conducive to the absorption of root to nutrient, is convenient to control rice shoot growing way without base fertilizer, and knot potato is even.
4. according to the biological property of potato, in net canopy, carry out alternating temperature management, be conducive to the formation of micro potato and expand.
5. ozone sterilization, avoided micro potato produce in use bactericide control germ occur time, the pollution of agricultural chemicals to environment.
6. the use of CO 2 gas fertilizer, has overcome the inside and outside gas exchangeability of net canopy poor, leaf photosynthesis insufficient raw material, make the indoor detoxification rice shoot of canopy under temp. and humidity adapt circumstance, photosynthesis is vigorous, and micro potato is neat, stem tuber heavily increases, and micro potato potato seed quality improves.
Within 7.75～80 days, kill the seedling micro potato of gathering, guarantee the physiological age that micro potato is definitely young, vitality is vigorous.
Good effect of the present invention: guarantee the virus infection that in hardening, field planting and subsequent the process of growth in micro potato production, tape kissing bug is not amboceptor, improve the aeration condition of the upper matrix of cultivation bed, micro potato potato seed quality improves, and knot potato is even, avoids the pollution of environment simultaneously; The present invention, under net canopy condition, utilizes soilless substyate culture, breeds the small stem tuber of 5～15 grams.Potato virus-free micro-seed be solve because of virus infections cause kind of sexual involution for production virus-free seed potato important way.
Embodiment
The present invention will be further described by the following examples.
In embodiment, each processing step concrete operations are as follows:
1. breeding facility selects
The facility of producing toxicity micro potato is arch-shaped steel frame construction plastic tunnel, is called for short net canopy, and fly net is set, and hangs polychrome dielectric fluke plate, and ozone for preventing and controlling machine and Rui Te A type carbonic acid gas growing aid are set;
2. hardening, transplanting and field planting
Hardening: detect and be accredited as virus-free bottle seedling through enzyme linked immunological virus, through expansion numerous switching 20～25d seedling age in blake bottle, high 5～8cm, the bottle seedling of 5～6 blades, moves on in net canopy, is placed on furrow bottle spacing 5cm; Field planting is opened sealed membrane in first 3～5 days, and tender seedling is contacted with the external world gradually, improves adaptive capacity;
Transplant: first make and the unsettled liftoff cultivation bed of ground isolation, in net canopy, build apart from ground 20cm with brick, the cultivation bed of wide 140cm, at the bottom of making bed of asbestos shingle, bedside edge is used brick limit, bed height 15cm; In bed, fill the thick vermiculite matrix through high-temperature roasting of 7-9cm, do not execute any base fertilizer, after leveling, plant before seedling, with 800 times of liquid of thiophanate methyl, ten thousand times of liquid of 70% sharp victory WS Diacloden 3-4, ground sterilization in net canopy; Adopt unsettled liftoff cultivation bed, cultivation seedling initial stage temperature is higher 2～3 DEG C than ground connection cultivation bed tempertaure;
Transplant planting: when the stable field planting during by 4～6 DEG C of ground temperature in net canopy, first from blake bottle, detoxic seedling is taken out before transplanting, wash away root medium with clear water; Adopt spacing in the rows × line-spacing=5cm × 7.5cm, i.e. 250 strains/m
2, single cave individual plant, presss from both sides the root of medium and seedling with tweezers when cuttage, finger presses detoxic seedling base portion, and seedling waters after being combined with matrix and closely planting seedling; The outer covered with sunshade net of net canopy, the slow Miao Yizhou that shelters from heat or light, prevents that direct sunlight shines seedling, after transplanted seedling grows new root after one week, progressively removes sunshade net; Soil humidity is controlled at 70%～80% left and right; After cultivation seedling, after 7～10 days 80% seedling rootings, start spray nutritious liquor;
3. nutrient solution prescription and fertilising
After field planting 7～21 days, adopt formula mg/kg: nitrate of lime 100, potassium nitrate 1034, magnesium sulfate 490, potassium dihydrogen phosphate 348, ammonium sulfate 170, calcium chloride 150, EDTA-Na 37.25, ferrous sulfate 27.85, copper sulphate 100; Filled with and execute one time of nutrition liquid every 5 days;
After field planting 3 weeks, impose fertilizer type and concentration and 667m
2fertilizer amount (pouring)
After field planting, in 21～75 days, impose foliage fertilizer one time every 10 days blade faces;
4. net canopy temperature treatment
In net canopy, detoxic seedling is transplanted latter one week interior air temperature control at 25～30 DEG C, exceedes 30 DEG C of coolings of leaking informaton; Seedling Stage is that before the 6th leaf or the 8th leaf launch, temperature is controlled between 20～25 DEG C, and potato tubers forms phase temperature and is controlled between 15～20 DEG C, and at expanding stage, potato needs higher day temperature, 18～20 DEG C, 12～14 DEG C of lower nocturnal temperatures;
5. press seedling, train vermiculite and cut point
In the time that rice shoot grows to 10 leaves, press seedling,, at 2～3 leaf places, top of seedling seedling is overwhelmed on medium with forefinger, cover matrix 2cm above, bed surface exposes 2～3 leaves; In the time that reaching 7 leaf, 80% plant leaf carries out training vermiculite the 1st time, training vermiculite 3～4 times in vegetative period, and thickness reaches 5～6cm, each training vermiculite is only trained two stipes of base portion, when field planting 7 weeks, cut off apical growing point, making axillary bud sprouting is side shoot, and seedling growing height is controlled at 25～30cm;
6. ozone-air sterilization and nutrient solution sterilization
Ozone for preventing and controlling machine hangs on net canopy central authorities skeleton, the exhaust outlet of two anemostat one end and ozone main frame joins, carry out electrion by the air in suction machine and make air ozonisation, the air of ozonisation is exported by air pump in machine again, and spreads out equably along the plastics trunnion that is laid on net canopy space; Service time: after the slow seedling of field planting, bring into use, the morning 8:00～9:00, afternoon 17:00～18:00, micro potato is gathered and is stopped the last week;
7. carbon dioxide fertilization
After the slow seedling of detoxic seedling field planting, blade grows to and approaches into age when size, and field planting, after 3 weeks, starts to enrich CO 2 gas fertilizer; Raw material is carbonic hydroammonium, and generation gas apparatus used is auspicious special A type carbonic acid gas growing aid; When every morning 8:00～11:00, before ventilation, stop using;
8. micro potato is gathered
When plant lower blade reaches 1/3rd flavescence, can gather, be controlled at 75-80 days results vegetative period, after gathering, be placed on ventilating and cooling place airing 7～10d; Pack seed packet into, post label, put cryopreservation under 2～4 DEG C of conditions in storage.
Described net canopy arranges fly net, selects the high-quality fly net in 60 order apertures to hide shed, is fixed on skeleton, obturages and can not leave gap, extends to ground from lower air port; The buffered gate that double-deck fly net forms is established in import and export.
Described polychrome dielectric fluke plate, is made up of high voltage source, substrate, dielectric coat, yellow, blue wear-resistant protective layer; The discrepancy place of opening the door inner side at fly net head-on hangs a polychrome dielectric fluke plate, utilizes adult love look, adopts colour table to add dielectric adsorption technology the adult of insect is attracted to and on plate, cements and kill; Every 20m in fly net
2put a polychrome dielectric fluke plate, polychrome dielectric fluke plate is suspended on to rice shoot top, bottom exceeds plant l0cm～15cm, traps and kills the adult that slips through the net, and polychrome dielectric fluke plate face is clean with wet cloth erasing in time.
Claims (3)
1. an integrated net canopy potato detoxicating micro potato mating system, is characterized in that comprising following processing step: comprise following processing step: 1. breed facility and select; 2. hardening, transplanting and field planting; 3. nutrient solution prescription and fertilising; 4. net canopy temperature treatment; 5. press seedling, train vermiculite and cut point; 6. ozone-air sterilization and nutrient solution sterilization; 7. carbon dioxide fertilization; 8. micro potato is gathered;
Each processing step concrete operations are as follows:
1. breeding facility selects
The facility of producing toxicity micro potato is arch-shaped steel frame construction plastic tunnel, is called for short net canopy, and fly net is set, and hangs polychrome dielectric fluke plate, and ozone for preventing and controlling machine and Rui Te A type carbonic acid gas growing aid are set;
2. hardening, transplanting and field planting
Hardening: detect and be accredited as virus-free bottle seedling through enzyme linked immunological virus, switching is expanded numerous in blake bottle, reaches 20～25d seedling age, and high 5～8cm, when 5～6 leaves, moves on in net canopy, is placed on furrow, waters foot permeable, bottle spacing 5cm; Field planting is opened sealed membrane in first 3～5 days, and tender seedling is contacted with the external world gradually, improves adaptive capacity;
Transplant: first make and the unsettled liftoff cultivation bed of ground isolation, in net canopy, build apart from ground 20cm with brick, the cultivation bed of wide 140cm, at the bottom of making bed of asbestos shingle, bedside edge is used brick limit, a thickness 15cm; In bed, fill the thick vermiculite matrix through high-temperature roasting of 7-9cm, do not execute any base fertilizer, after leveling, plant before seedling, with 800 times of liquid of thiophanate methyl, ten thousand times of liquid of 70% sharp victory WS Diacloden 3-4, ground sterilization in net canopy; Cultivation bed adopts unsettled liftoff cultivation bed, and cultivation seedling initial stage temperature is higher 2～3 DEG C than ground connection cultivation bed tempertaure;
Transplant planting: field planting in the time that ground temperature reaches 4～6 DEG C in net canopy, before transplanting, first from blake bottle, detoxic seedling is taken out, wash away root medium with clear water; Adopt spacing in the rows × line-spacing=5cm × 7.5cm, i.e. 250 strains/m
2, single cave individual plant, presss from both sides the root of medium and seedling with tweezers when cuttage, finger presses detoxic seedling base portion, and seedling is combined with matrix closely, after cultivation seedling, waters; The outer covered with sunshade net of net canopy, the slow Miao Yizhou that shelters from heat or light, prevents that direct sunlight shines seedling, after transplanted seedling grows new root after one week, progressively removes sunshade net; Soil humidity is controlled at 70%～80% left and right; After cultivation seedling, after 7～10 days 80% seedling rootings, start spray nutritious liquor;
3. nutrient solution prescription and fertilising
After field planting 7～21 days, adopt formula mg/kg: nitrate of lime 100, potassium nitrate 1034, magnesium sulfate 490, potassium dihydrogen phosphate 348, ammonium sulfate 170, calcium chloride 150, EDTA-Na 37.25, ferrous sulfate 27.85, copper sulphate 100; Filled with and execute one time of nutrition liquid every 5 days;
After field planting, in 21～75 days, impose foliage fertilizer one time every 10 days blade faces;
4. net canopy temperature treatment
In net canopy, detoxic seedling is transplanted latter one week interior air temperature control at 25～30 DEG C, exceedes 30 DEG C of coolings of leaking informaton; Seedling Stage is that before the 6th leaf or the 8th leaf launch, temperature is controlled between 20～25 DEG C, and potato tubers forms phase temperature and is controlled between 15～20 DEG C, and at expanding stage, potato needs higher day temperature, 18～20 DEG C, 12～14 DEG C of lower nocturnal temperatures;
5. press seedling, train vermiculite and cut point
In the time that rice shoot grows to 10 leaves, press seedling,, at 2～3 leaf places, top of seedling seedling is overwhelmed on medium with forefinger, cover matrix 2cm above, bed surface exposes 2～3 leaves; In the time that reaching 7 leaf, 80% plant leaf carries out training vermiculite the 1st time, training vermiculite 3～4 times in vegetative period, and thickness reaches 5～6cm, each training vermiculite is only trained two stipes of base portion, when field planting 7 weeks, cut off apical growing point, making axillary bud sprouting is side shoot, and seedling growing height is controlled at 25～30cm;
6. ozone-air sterilization and nutrient solution sterilization
Ozone for preventing and controlling machine hangs on net canopy central authorities skeleton, the exhaust outlet of two anemostat one end and ozone main frame joins, carry out electrion by the air in suction machine and make air ozonisation, the air of ozonisation is exported by air pump in machine again, and spreads out equably along the plastics trunnion that is laid on net canopy space; Service time: after the slow seedling of field planting, bring into use, the morning 8:00～9:00, afternoon 17:00～18:00, micro potato is gathered and is stopped the last week;
7. carbon dioxide fertilization
After the slow seedling of detoxic seedling field planting, blade grows to and approaches into age when size, and field planting, after 3 weeks, starts to enrich CO 2 gas fertilizer; Raw material is carbonic hydroammonium, and generation gas apparatus used is auspicious special A type carbonic acid gas growing aid; When every morning 8:00～11:00, before ventilation, stop using;
8. micro potato is gathered
When plant lower blade reaches 1/3rd flavescence, can gather, be controlled at 75-80 days results vegetative period, after gathering, be placed on ventilating and cooling place airing 7～10d; Pack seed packet into, post label, put cryopreservation under 2～4 DEG C of conditions in storage.
2. the integrated net canopy of one according to claim 1 potato detoxicating micro potato mating system, it is characterized in that described net canopy arranges fly net, select the high-quality fly net in 60 order apertures to hide shed, be fixed on skeleton, obturage and can not leave gap, extend to ground from lower air port; The buffered gate that double-deck fly net forms is established in import and export.
3. the integrated net canopy of one according to claim 2 potato detoxicating micro potato mating system, is characterized in that described polychrome dielectric fluke plate, is made up of high voltage source, substrate, dielectric coat, yellow, blue wear-resistant protective layer; The discrepancy place of opening the door inner side at fly net head-on hangs a polychrome dielectric fluke plate, utilizes adult love look, adopts colour table to add dielectric adsorption technology the adult of insect is attracted to and on plate, cements and kill; Every 20m in fly net
2put a polychrome dielectric fluke plate, polychrome dielectric fluke plate is suspended on to rice shoot top, bottom exceeds plant l0cm～15cm, traps and kills the adult that slips through the net.
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| CN105210600A (en) * | 2015-07-08 | 2016-01-06 | 甘肃凯凯农业科技发展股份有限公司 | Virus-free basic potato seed hot-house culture method |
| CN105432180A (en) * | 2015-12-08 | 2016-03-30 | 安徽省农业科学院园艺研究所 | Ozone sterilization technology of seed potatoes |
| CN107027625B (en) * | 2017-03-29 | 2019-05-31 | 广西钦州胜腾商务有限公司 | A kind of method for tissue culture of Lonicera standishii |
| CN107646362A (en) * | 2017-09-27 | 2018-02-02 | 界首市五季果源家庭农场 | A kind of method of tribute chrysanthemum lateral bud children stem micro cuttage |
| CN109744113A (en) * | 2019-01-30 | 2019-05-14 | 云南洪邦薯业发展有限公司 | A kind of implantation methods of micro potato |
| CN109997669A (en) * | 2019-03-28 | 2019-07-12 | 金华市农业科学研究院 | A method of cultivating sweet potato micro potato seedling |
| CN111448962A (en) * | 2020-05-06 | 2020-07-28 | 云南泵龙马铃薯种植有限公司 | Breeding method of virus-free miniature potatoes |
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Effective date of registration: 20210510 Address after: 027099 gongbaolage Sumu baorihaote Gacha, Taipusi Banner, Xilin Gol League, Inner Mongolia Autonomous Region Patentee after: Inner Mongolia gerid Potato Seed Industry Group Co., Ltd Address before: 063000 Tangshan Vocational and technical college, Tangshan City, Hebei Province Patentee before: Liu Quanguo Patentee before: Chang Shengwang Patentee before: Li Donghu Patentee before: Zhen Xiaoming Patentee before: Yang Jun Patentee before: Gao Yan Patentee before: Gao Junqiang Patentee before: Li Zhiqiang Patentee before: Wang Xin Patentee before: Chen Hao |